Pkm

Summary

Gene Symbol: Pkm
Description: pyruvate kinase, muscle
Alias: AA414905, AL024370, AL024424, Pk-2, Pk-3, Pk3, Pkm2, pyruvate kinase PKM, pyruvate kinase 3, pyruvate kinase isozymes M1/M2, pyruvate kinase muscle isozyme
Species: mouse

Top Publications

  1. pmc PPARγ contributes to PKM2 and HK2 expression in fatty liver
    Ganna Panasyuk
    INSERM, U845, Paris 75015, France
    Nat Commun 3:672. 2012
  2. doi The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth
    Heather R Christofk
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 452:230-3. 2008
  3. doi Pyruvate kinase M2 is a phosphotyrosine-binding protein
    Heather R Christofk
    Department of Systems Biology
    Nature 452:181-6. 2008
  4. pmc Germline loss of PKM2 promotes metabolic distress and hepatocellular carcinoma
    Talya L Dayton
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 30:1020-33. 2016
  5. doi PTB deficiency causes the loss of adherens junctions in the dorsal telencephalon and leads to lethal hydrocephalus
    Takayuki Shibasaki
    Laboratory of Developmental Genetics, Center for Experimental Medicine and Systems Biology, University of Tokyo, Tokyo, Japan
    Cereb Cortex 23:1824-35. 2013
  6. pmc Transcriptomic analysis of PNN- and ESRP1-regulated alternative pre-mRNA splicing in human corneal epithelial cells
    Jeong Hoon Joo
    Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    Invest Ophthalmol Vis Sci 54:697-707. 2013
  7. pmc Reexpression of pyruvate kinase M2 in type 1 myofibers correlates with altered glucose metabolism in myotonic dystrophy
    Zhihua Gao
    Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 110:13570-5. 2013
  8. pmc STAT6 promotes bi-directional modulation of PKM2 in liver and adipose inflammatory cells in rosiglitazone-treated mice
    Tatjana Sajic
    Department of Cellular Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1 rue Michel Servet, Geneva 4, Switzerland
    Sci Rep 3:2350. 2013
  9. pmc Direct measurements of oscillatory glycolysis in pancreatic islet β-cells using novel fluorescence resonance energy transfer (FRET) biosensors for pyruvate kinase M2 activity
    Matthew J Merrins
    From the Department of Pharmacology and Brehm Center for Diabetes Research, University of Michigan Medical School, Ann Arbor, Michigan 48105
    J Biol Chem 288:33312-22. 2013
  10. doi Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B
    Xiaoyun Wu
    State Key Laboratory of Virology, the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China
    FEBS Lett 582:2155-60. 2008

Research Grants

Scientific Experts

  • Steven L McKnight
  • Keiichi Ishihara
  • Saima Zafar
  • Scott J Parkinson
  • Jeong Hoon Joo
  • Matthew G Vander Heiden
  • William J Israelsen
  • Lewis C Cantley
  • Talya L Dayton
  • Aaron M Hosios
  • Heather R Christofk
  • Clary B Clish
  • Jiansheng Xie
  • Suijun Wang
  • Meredith L Rees
  • Eva M Palsson-McDermott
  • Sophia Y Lunt
  • Dan Y Gui
  • Qiyun Tang
  • Tyler Jacks
  • Shawn M Davidson
  • Gary Bellinger
  • Ashish Kumar
  • Ken J Lindsay
  • Brian P Fiske
  • Ying Hua Wang
  • Mohd Askandar Iqbal
  • Lei Lv
  • Tatjana Sajic
  • Zhihua Gao
  • Matthew J Merrins
  • Takayuki Shibasaki
  • Ganna Panasyuk
  • Qian Sun
  • Jianxin Xie
  • Cynthia V Clower
  • Taro Hitosugi
  • Gilles A Spoden
  • John M Asara
  • Jungwoon Lee
  • Xiaoyun Wu
  • Akemi Hoshino
  • Linda S Kaltenbach
  • Michelle Krisfalusi
  • Jiyoon Ryu
  • Vasilena Gocheva
  • Xun Hu
  • Arjun Bhutkar
  • Roderick T Bronson
  • Jindan Yu
  • Chunyan Dai
  • Kathryn M Miller
  • Yuuki Asai
  • Alba Luengo
  • Yuanteng Li
  • Martin Ogrodzinski
  • Hua Man
  • Anne M Curtis
  • Jian an Bai
  • Vinayak Muralidhar
  • Sarah Maria Fendt
  • Joseph Keane
  • Jian Kang Jiang
  • Hongxia Liu
  • Yongwei Qin
  • Stefan Christen
  • Runzhou Ni
  • Craig Thomas
  • Susan R Quinn
  • Daniel P Hollern
  • Gautam Goel
  • Raquel Domingo-Fernández
  • Ramnik J Xavier
  • Eran R Andrechek
  • Vivian Hecht
  • Yun Zhang
  • Gregory Stephanopoulos
  • Anh T Dinh
  • MATTHEW VANDER HEIDEN
  • Paula N Marín Acevedo
  • Clary Clish
  • Quanzhong Li
  • Kali Xu
  • Dale J Hamilton
  • Yong Su
  • Jain Kang Jiang
  • Weiwei Xia
  • Qianqian Ji
  • Yanfang Wang
  • Heinrich Taegtmeyer

Detail Information

Publications51

  1. pmc PPARγ contributes to PKM2 and HK2 expression in fatty liver
    Ganna Panasyuk
    INSERM, U845, Paris 75015, France
    Nat Commun 3:672. 2012
    ....
  2. doi The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth
    Heather R Christofk
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 452:230-3. 2008
    ..These results demonstrate that M2 expression is necessary for aerobic glycolysis and that this metabolic phenotype provides a selective growth advantage for tumour cells in vivo...
  3. doi Pyruvate kinase M2 is a phosphotyrosine-binding protein
    Heather R Christofk
    Department of Systems Biology
    Nature 452:181-6. 2008
    ..have made the observation that an enzyme involved in glycolysis, the human M2 (fetal) isoform of pyruvate kinase (PKM2), binds directly and selectively to tyrosine-phosphorylated peptides...
  4. pmc Germline loss of PKM2 promotes metabolic distress and hepatocellular carcinoma
    Talya L Dayton
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 30:1020-33. 2016
    Alternative splicing of the Pkm gene product generates the PKM1 and PKM2 isoforms of pyruvate kinase (PK), and PKM2 expression is closely linked to embryogenesis, tissue regeneration, and cancer...
  5. doi PTB deficiency causes the loss of adherens junctions in the dorsal telencephalon and leads to lethal hydrocephalus
    Takayuki Shibasaki
    Laboratory of Developmental Genetics, Center for Experimental Medicine and Systems Biology, University of Tokyo, Tokyo, Japan
    Cereb Cortex 23:1824-35. 2013
    ..5. These findings demonstrated that PTB depletion in the dorsal telencephalon is causally involved in the development of HC and that PTB is important for the maintenance of AJs in the NSCs of the dorsal telencephalon. ..
  6. pmc Transcriptomic analysis of PNN- and ESRP1-regulated alternative pre-mRNA splicing in human corneal epithelial cells
    Jeong Hoon Joo
    Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    Invest Ophthalmol Vis Sci 54:697-707. 2013
    ..We investigated the impact of PININ (PNN) and epithelial splicing regulatory protein 1 (ESRP1) on alternative pre-mRNA splicing in the corneal epithelial context...
  7. pmc Reexpression of pyruvate kinase M2 in type 1 myofibers correlates with altered glucose metabolism in myotonic dystrophy
    Zhihua Gao
    Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 110:13570-5. 2013
    ..Here we report that the embryonic M2 isoform of pyruvate kinase (PKM2), a key enzyme contributing to the Warburg effect in cancer, is significantly induced in DM1 tissue and mouse ..
  8. pmc STAT6 promotes bi-directional modulation of PKM2 in liver and adipose inflammatory cells in rosiglitazone-treated mice
    Tatjana Sajic
    Department of Cellular Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1 rue Michel Servet, Geneva 4, Switzerland
    Sci Rep 3:2350. 2013
    ..we compared liver proteomes of RSG-treated wild type and STAT6-deficient mice and we identified pyruvate kinase M2 (PKM2), a glycolysis and proliferation-regulating enzyme that displayed STAT6-dependent expression...
  9. pmc Direct measurements of oscillatory glycolysis in pancreatic islet β-cells using novel fluorescence resonance energy transfer (FRET) biosensors for pyruvate kinase M2 activity
    Matthew J Merrins
    From the Department of Pharmacology and Brehm Center for Diabetes Research, University of Michigan Medical School, Ann Arbor, Michigan 48105
    J Biol Chem 288:33312-22. 2013
    ..in mitochondrial redox potential stimulated by 10 mm glucose are in phase with glycolytic efflux through PKM2, measured simultaneously from neighboring islet β-cells expressing PKAR...
  10. doi Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B
    Xiaoyun Wu
    State Key Laboratory of Virology, the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China
    FEBS Lett 582:2155-60. 2008
    ..Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells...
  11. pmc PKM2 isoform-specific deletion reveals a differential requirement for pyruvate kinase in tumor cells
    William J Israelsen
    Koch Institute for Integrative Cancer Research at Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 155:397-409. 2013
    The pyruvate kinase M2 isoform (PKM2) is expressed in cancer and plays a role in regulating anabolic metabolism...
  12. pmc Mitogenic and oncogenic stimulation of K433 acetylation promotes PKM2 protein kinase activity and nuclear localization
    Lei Lv
    Key Laboratory of Molecular Medicine, Ministry of Education, and Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, People s Republic of China Molecular and Cell Biology Lab, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, People s Republic of China School of Life Sciences, Fudan University 200032, People s Republic of China
    Mol Cell 52:340-52. 2013
    Alternative splicing of the PKM2 gene produces two isoforms, M1 and M2, which are preferentially expressed in adult and embryonic tissues, respectively...
  13. doi Anchorless 23-230 PrPC interactomics for elucidation of PrPC protective role
    Saima Zafar
    Department of Neurology, University Medical Center Göttingen UMG, Robert Koch Str 40, 37075, Gottingen, Germany
    Mol Neurobiol 49:1385-99. 2014
    ..Out of the 23 proteins, one novel protein, pyruvate kinase isozymes M1/M2 (PKM2), exhibited a potential interaction with the anchorless Δ23-230 form of PrPC...
  14. pmc Missense mutations in pyruvate kinase M2 promote cancer metabolism, oxidative endurance, anchorage independence, and tumor growth in a dominant negative manner
    Mohd Askandar Iqbal
    From the National Centre of Applied Human Genetics, School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067 India
    J Biol Chem 289:8098-105. 2014
    ..Cells stably expressing exogenous wild-type or mutant PKM2 (K422R or H391Y) or co-expressing both wild type and mutant (PKM2-K422R or PKM2-H391Y) were assessed for cancer ..
  15. doi Please keep me 2uned to PKM2
    Steven L McKnight
    Department of Biochemistry, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9152, USA Electronic address
    Mol Cell 53:683-4. 2014
    In this issue of Molecular Cell, Keller et al. (2014) found that binding of the metabolite SAICAR to PKM2 induces the protein kinase activity of an enzyme normally designed to terminate the glycolytic pathway.
  16. doi Pyruvate kinase M2 regulates apoptosis of intestinal epithelial cells in Crohn's disease
    Qiyun Tang
    Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, China
    Dig Dis Sci 60:393-404. 2015
    Pyruvate kinase M2 (PKM2), a key glycolytic enzyme, is involved in multiple cellular processes including apoptosis...
  17. doi Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development
    Ashish Kumar
    Biochemistry and Molecular Biology Laboratory, Brain Research Centre, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India
    Int J Dev Neurosci 38:138-46. 2014
    ....
  18. pmc Cell-state-specific metabolic dependency in hematopoiesis and leukemogenesis
    Ying Hua Wang
    Center for Regenerative Medicine and Cancer Center, Massachusetts General Hospital, Boston, MA 02114, USA Harvard Stem Cell Institute, Cambridge, MA 02114, USA Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 158:1309-23. 2014
    ..We find that a deficiency in the M2 pyruvate kinase isoform (PKM2) reduces the levels of metabolic intermediates important for biosynthesis and impairs progenitor function without ..
  19. doi Comparative proteomic profiling reveals aberrant cell proliferation in the brain of embryonic Ts1Cje, a mouse model of Down syndrome
    K Ishihara
    Department of Pathological Biochemistry, Kyoto Pharmaceutical University, 5 Misasagi Nakauchi cho, Yamashina ku, Kyoto 607 8414, Japan Laboratory for Neurogenetics, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan Electronic address
    Neuroscience 281:1-15. 2014
    ....
  20. pmc Pyruvate kinase and aspartate-glutamate carrier distributions reveal key metabolic links between neurons and glia in retina
    Ken J Lindsay
    Departments of Biochemistry
    Proc Natl Acad Sci U S A 111:15579-84. 2014
    ..Müller glia compensate for their unique metabolic adaptations by using lactate and aspartate from neurons as surrogates for their missing PK and AGC1. ..
  21. pmc Pyruvate kinase isoform expression alters nucleotide synthesis to impact cell proliferation
    Sophia Y Lunt
    Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA Department of Physiology, Michigan State University, East Lansing, MI 48824, USA
    Mol Cell 57:95-107. 2015
    ..The influence of pyruvate kinase isoforms on tumor cells has been extensively studied, but whether PKM2 is required for normal cell proliferation is unknown...
  22. pmc Pyruvate kinase M2 regulates Hif-1α activity and IL-1β induction and is a critical determinant of the warburg effect in LPS-activated macrophages
    Eva M Palsson-McDermott
    School of Biochemistry and Immunology, Trinity Biomedical Science Institute, Trinity College Dublin, Dublin 2, Ireland
    Cell Metab 21:65-80. 2015
    ..We here show that LPS induces expression of the key metabolic regulator Pyruvate Kinase M2 (PKM2)...
  23. pmc A PKM2 signature in the failing heart
    Meredith L Rees
    Department of Internal Medicine, Division of Cardiology, The University of Texas Medical School at Houston, 6431 Fannin Street, MSB 1 220, Houston, TX 77030, USA
    Biochem Biophys Res Commun 459:430-6. 2015
    ..Additionally, we observed a shift in the enzyme pyruvate kinase from the adult M1 (PKM1) isoform to the fetal M2 (PKM2) isoform, a hallmark of the Warburg Effect...
  24. pmc Lack of Evidence for PKM2 Protein Kinase Activity
    Aaron M Hosios
    Koch Institute for Integrative Cancer Research at Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 59:850-7. 2015
    The role of pyruvate kinase M2 (PKM2) in cell proliferation is controversial...
  25. pmc Pyruvate kinase, muscle isoform 2 promotes proliferation and insulin secretion of pancreatic β-cells via activating Wnt/CTNNB1 signaling
    Suijun Wang
    Department of Endocrinology and Metabolism, Henan Provincial People s Hospital, Zhengzhou University Zhengzhou 450003, P R China
    Int J Clin Exp Pathol 8:14441-8. 2015
    ..Pyruvate kinase, muscle isoform 2 (PKM2) is a potential modulator of insulin secretion in β-cells...
  26. pmc Evidence That Does Not Support Pyruvate Kinase M2 (PKM2)-catalyzed Reaction as a Rate-limiting Step in Cancer Cell Glycolysis
    Jiansheng Xie
    From the Cancer Institute a Key Laboratory for Cancer Prevention and Intervention, China National Ministry of Education, The Second Affiliated Hospital, 310009 Hangzhou and the Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016 Hangzhou, China
    J Biol Chem 291:8987-99. 2016
    It has been recognized that the rate-limiting function of pyruvate kinase M2 (PKM2) in glycolysis plays an important role in distributing glycolytic intermediates for anabolic and catabolic purposes in cancer cells...
  27. pmc Identification of PKCzetaII: an endogenous inhibitor of cell polarity
    Scott J Parkinson
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, UK
    EMBO J 23:77-88. 2004
    ..The data demonstrate a regulatory role for PKCzetaII in the maintenance of cell transformation and the development of cell polarity...
  28. pmc Annotation and analysis of 10,000 expressed sequence tags from developing mouse eye and adult retina
    Jindan Yu
    Ophthalmology and Visual Science, University of Michigan, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Genome Biol 4:R65. 2003
    ..We have generated and analyzed 10,000 expressed sequence tags (ESTs) from three mouse eye tissue cDNA libraries: embryonic day 15.5 (M15E) eye, postnatal day 2 (M2PN) eye and adult retina (MRA)...
  29. pmc The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism
    Cynthia V Clower
    Division of Signal Transduction, Department of Systems Biology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:1894-9. 2010
    ..These findings extend the links between alternative splicing and cancer, and begin to define some of the factors responsible for the switch to aerobic glycolysis...
  30. ncbi Characterization of genes which exhibit reduced expression during the retinoic acid-induced differentiation of F9 teratocarcinoma cells: involvement of cyclin D3 in RA-mediated growth arrest
    T N Faria
    Department of Pharmacology, Cornell University Medical College, New York, NY 10021, USA
    Mol Cell Endocrinol 143:155-66. 1998
    ....
  31. ncbi The genes coding for pyruvate kinase (M2) and mannosephosphate isomerase are linked in man and mouse
    P A Lalley
    Cytogenet Cell Genet 22:581-4. 1978
  32. ncbi The Pk-3 gene determines both the heart, M1, and the kidney, M2, pyruvate kinase isozymes in the mouse; and a simple electrophoretic method for separating phosphoglucomutase-3
    J Peters
    Biochem Genet 22:1047-63. 1984
    ..We also report a simple method for separating phosphoglucomutase-3 (PGM-3) by electrophoresis on cellulose acetate plates...
  33. ncbi Genetic mapping in mammals: chromosome map of domestic cat
    S J O'Brien
    Science 216:257-65. 1982
    ..Many of these same linkage groups have not been conserved in the murine genome. The genetic and evolutionary implications of comparative mapping analysis among mammalian species are discussed...
  34. ncbi A variation in mouse kidney pyruvate kinase activity determined by a mutant gene on chromosome 9
    F M Johnson
    Genet Res 37:123-31. 1981
  35. ncbi Polymorphism of kidney pyruvate kinase in the mouse is determined by a gene, Pk-3, on chromosome 9
    J Peters
    Biochem Genet 19:757-69. 1981
    ..In addition, our results indicate that the muscle (M1) and kidney (M2) pyruvate kinase isozymes share at least one genetic determinant and may in fact be determined by the same structural gene...
  36. ncbi Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation
    S Izumi
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biochim Biophys Acta 1267:135-8. 1995
    ..Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages...
  37. ncbi Cloning and characterization of genes expressed during gametogenesis of female and male mice
    D M López-Alañón
    Centro de Investigaciones Biologicas, Velazquez, Madrid, Spain
    J Reprod Fertil 103:323-9. 1995
    ..Additional specific, gametic transcripts were also detected...
  38. ncbi Identification of genes showing altered expression in preimplantation and early postimplantation parthenogenetic embryos
    M Mann
    Department of Zoology, University of Toronto, Ontario, Canada
    Dev Genet 17:223-32. 1995
    ....
  39. ncbi Induction by glucose of genes coding for glycolytic enzymes in a pancreatic beta-cell line (INS-1)
    E Roche
    Molecular Nutrition Unit, Department of Nutrition, University of Montreal, H3C 3J7 Montreal, Quebec, Canada
    J Biol Chem 272:3091-8. 1997
    ....
  40. ncbi Gene expression of mouse M1 and M2 pyruvate kinase isoenzymes correlates with differential poly[A] tract extension of their mRNAs during the development of spermatogenesis
    O de Luis
    Department of Cell and Developmental Biology, Centro de Investigaciones Biologicas C S I C, Madrid, Spain
    Biochim Biophys Acta 1396:294-305. 1998
    ..These results suggest that changes in the length of the poly[A] tail of transcripts are associated with differential expression of both regulated isoenzymes during testicular development...
  41. pmc Mammalian target of rapamycin up-regulation of pyruvate kinase isoenzyme type M2 is critical for aerobic glycolysis and tumor growth
    Qian Sun
    State Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences and School of Basic Medicine, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100005, China
    Proc Natl Acad Sci U S A 108:4129-34. 2011
    ..For a largely unknown regulatory mechanism, a rate-limiting glycolytic enzyme pyruvate kinase M2 (PKM2) isoform is exclusively expressed in embryonic, proliferating, and tumor cells, and plays an essential role in ..
  42. ncbi Insulin stimulates expression of the pyruvate kinase M gene in 3T3-L1 adipocytes
    Yuuki Asai
    Department of Applied Molecular Biosciences, Nagoya University Graduate School of Bioagricultural Sciences, Chikusa ku, Nagoya 464 8601, Japan
    Biosci Biotechnol Biochem 67:1272-7. 2003
    M2-type pyruvate kinase (M2-PK) mRNA is produced from the PKM gene by an alternative RNA splicing in adipocytes. We found that insulin increased the level of M2-PK mRNA in 3T3-L1 adipocytes in both time- and dose-dependent manners...
  43. ncbi Proteomic identification of the TRAF6 regulation of vacuolar ATPase for osteoclast function
    Jiyoon Ryu
    Department of Cell and Developmental Biology, Dental Research Institute, College of Dentistry, Seoul National University, Seoul, Korea
    Proteomics 5:4152-60. 2005
    ..Our study identified V-ATPase as a TRAF6-binding protein using a proteomics strategy and proved a direct link between these two important molecules for osteoclast function...
  44. ncbi Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa
    Michelle Krisfalusi
    Laboratory for Reproductive Biology, University of North Carolina School of Medicine, NC 27599, USA
    Biol Reprod 75:270-8. 2006
    ..These data support a role for the fibrous sheath as a scaffold for anchoring multiple glycolytic enzymes along the length of the flagellum to provide a localized source of ATP that is essential for sperm motility...
  45. ncbi Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase
    Akemi Hoshino
    Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 282:17706-11. 2007
    ....
  46. doi Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription
    Jungwoon Lee
    Department of Life Science, Sogang University, Seoul 121 742, Republic of Korea
    Int J Biochem Cell Biol 40:1043-54. 2008
    ..MS (mass spectrometry) and isolated a novel Oct-4-interacting protein, pyruvate kinase type M2 (PKM2 or M2-PK)...
  47. doi Pyruvate kinase isoenzyme M2 is a glycolytic sensor differentially regulating cell proliferation, cell size and apoptotic cell death dependent on glucose supply
    Gilles A Spoden
    Department of Cell Metabolism and Differentiation, Institute for Biomedical Aging Research of the Austrian Academy of Sciences, Rennweg 10, 6020 Innsbruck, Austria
    Exp Cell Res 315:2765-74. 2009
    The glycolytic key regulator pyruvate kinase M2 (M2-PK or PKM2) can switch between a highly active tetrameric and an inactive dimeric form. The transition between the two conformations regulates the glycolytic flux in tumor cells...
  48. pmc Tyrosine phosphorylation inhibits PKM2 to promote the Warburg effect and tumor growth
    Taro Hitosugi
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Sci Signal 2:ra73. 2009
    ..We found that oncogenic forms of fibroblast growth factor receptor type 1 inhibit the pyruvate kinase M2 (PKM2) isoform by direct phosphorylation of PKM2 tyrosine residue 105 (Y(105))...
  49. ncbi Conservation of autosomal gene synteny groups in mouse and man
    P A Lalley
    Nature 274:160-3. 1978
  50. pmc Evidence for an alternative glycolytic pathway in rapidly proliferating cells
    Matthew G Vander Heiden
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 329:1492-9. 2010
    ..The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucose by aerobic glycolysis and contributes to anabolic metabolism...
  51. pmc Huntingtin interacting proteins are genetic modifiers of neurodegeneration
    Linda S Kaltenbach
    Prolexys Pharmaceuticals, Salt Lake City, Utah, United States of America
    PLoS Genet 3:e82. 2007
    ..These studies demonstrate that high-throughput screening for protein interactions combined with genetic validation in a model organism is a powerful approach for identifying novel candidate modifiers of polyglutamine toxicity...

Research Grants2

  1. MOLECULAR CONTROL OF MAMMALIAN ENERGY HOMEOSTASIS
    STEVEN MCKNIGHT; Fiscal Year: 2001
    ....
  2. GENETICS & BIOCHEMISTRY OF NEURONAL PAS DOMAIN PROTEINS
    STEVEN MCKNIGHT; Fiscal Year: 2008
    ..abstract_text> ..