Genomes and Genes
Gene Symbol: Ocln
Alias: AI503564, Ocl, occludin
Publications109 found, 100 shown here
- Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivoAmanda M Marchiando
Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
J Cell Biol 189:111-26. 2010..We have used in vivo imaging and transgenic mice expressing fluorescent-tagged occludin and ZO-1 fusion proteins to link occludin endocytosis to TNF-induced tight junction regulation...
- Use of surface plasmon resonance for real-time analysis of the interaction of ZO-1 and occludinA Schmidt
Institute of Molecular Pharmacology, Robert Rossle Strasse 10, 13125 Berlin, Germany
Biochem Biophys Res Commun 288:1194-9. 2001..spectroscopy was applied to study in real time, the interaction between the tight junction proteins ZO-1 and occludin. To imitate the morphology of tight junctions, a cytosolic tail of mouse occludin was immobilised at the sensor ..
- Perinatal lethality and endothelial cell abnormalities in several vessel compartments of fibulin-1-deficient miceG Kostka
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Mol Cell Biol 21:7025-34. 2001..The data indicate a strong but restricted abnormality in some endothelial compartments which, together with some kidney and lung defects, may be responsible for early death...
- Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1Olga V Volpert
Department of Urology and RH Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611, USA
Cancer Cell 2:473-83. 2002..This decrease was completely reversed by a TSP-1 neutralizing antibody. We conclude that TSP-1 is a major target for Id1 effects on angiogenesis...
- The cell adhesion molecule nectin-1 is critical for normal enamel formation in miceMartin J Barron
Faculty of Life Sciences, University of Manchester, Manchester, UK
Hum Mol Genet 17:3509-20. 2008..Importantly, our results show that integrity of the SI-ameloblast interface is essential for normal enamel mineralization...
- Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologuesY Ando-Akatsuka
Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
J Cell Biol 133:43-7. 1996b>Occludin has been identified from chick liver as a novel integral membrane protein localizing at tight junctions (Furuse, M., T. Hirase, M. Itoh, A. Nagafuchi, S. Yonemura, Sa. Tsukita, and Sh. Tsukita. 1993. J. Cell Biol. 123:1777-1788)...
- Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctionsM Saitou
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
J Cell Biol 141:397-408. 1998b>Occludin is the only known integral membrane protein of tight junctions (TJs), and is now believed to be directly involved in the barrier and fence functions of TJs...
- Subcellular distribution of tight junction-associated proteins (occludin, ZO-1, ZO-2) in rodent skinK Morita
Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
J Invest Dermatol 110:862-6. 1998b>Occludin is an integral membrane protein that is concentrated at tight junctions (zonulae occludentes) in simple epithelial cells...
- A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblastsM Furuse
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
J Cell Biol 143:391-401. 1998Three integral membrane proteins, clau- din-1, -2, and occludin, are known to be components of tight junction (TJ) strands. To examine their ability to form TJ strands, their cDNAs were introduced into mouse L fibroblasts lacking TJs...
- Complex phenotype of mice lacking occludin, a component of tight junction strandsM Saitou
Department of Cell Biology, Kyoto University Faculty of Medicine, Sakyo ku, Kyoto 606 8501, Japan
Mol Biol Cell 11:4131-42. 2000b>Occludin is an integral membrane protein with four transmembrane domains that is exclusively localized at tight junction (TJ) strands. Here, we describe the generation and analysis of mice carrying a null mutation in the occludin gene...
- The tight junction-specific protein occludin is a functional target of the E3 ubiquitin-protein ligase itchAndreas Traweger
Institute of Molecular Biology, Austrian Academy of Sciences, 5020 Salzburg, Austria
J Biol Chem 277:10201-8. 2002..Recent evidence confirms the notion that occludin, a four-pass integral plasma-membrane protein, is a functional component of the paracellular barrier...
- Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by SnailJunichi Ikenouchi
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Cell Sci 116:1959-67. 2003..Claudins and occludin are integral membrane proteins localized at tight junctions, which are responsible for establishing and ..
- Rab13 mediates the continuous endocytic recycling of occludin to the cell surfaceShinya Morimoto
Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770 8503, Japan
J Biol Chem 280:2220-8. 2005..b>Occludin and claudins are integral membrane proteins comprising TJ strands and are thought to be responsible for ..
- Epithelial transport and barrier function in occludin-deficient miceJ D Schulzke
Department of Gastroenterology, Campus Benjamin Franklin, Charité Universitary Medicine Berlin, Germany
Biochim Biophys Acta 1669:34-42. 2005This study aimed at functional characterization of the tight junction protein occludin using the occludin-deficient mouse model.
- Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cellsJunichi Ikenouchi
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Cell Biol 171:939-45. 2005..These findings indicate the critical function of tricellulin for formation of the epithelial barrier...
- Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cellsTatsuya Katsuno
Laboratory of Biological Science, Graduate School of Frontier Biosciences, and Graduate School of Medicine, Osaka University, Japan
Mol Biol Cell 19:2465-75. 2008..In conclusion, ZO-1 may be functionally important for cell remodeling and tissue organization in both the embryonic and extraembryonic regions, thus playing an essential role in embryonic development...
- Caveolae-mediated internalization of occludin and claudin-5 during CCL2-induced tight junction remodeling in brain endothelial cellsSvetlana M Stamatovic
Departments of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
J Biol Chem 284:19053-66. 2009..It investigates the potential role of selective internalization of TJ transmembrane proteins (occludin and claudin-5) in increased permeability of the brain endothelial barrier in vitro...
- CD44 regulates tight-junction assembly and barrier functionNina Kirschner
Department of Dermatology and Venerology, University Hospital Hamburg Eppendorf, Hamburg, Germany
J Invest Dermatol 131:932-43. 2011..Taken together, the results reveal an important function for CD44 in the assembly and function of TJs, suggesting their involvement in the skin barrier phenotype of CD44 KO mice...
- MEK kinase 1 regulates c-Jun phosphorylation in the control of corneal morphogenesisLin Zhang
Center for Environmental Genetics and Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH 45267 0056, USA
Mol Vis 9:584-93. 2003..To study the in vivo role of MEK kinase 1 (MEKK1) in corneal development...
- The tight junction protein occludin and the adherens junction protein alpha-catenin share a common interaction mechanism with ZO-1Sebastian L Müller
Forschungsinstitut fur Molekulare Pharmakologie, 13125 Berlin, Charite Campus Mitte, 10117 Berlin, Germany, and Charité Campus Benjamin Franklin, 12203 Berlin, Germany
J Biol Chem 280:3747-56. 2005..of interaction between junction organizing zona occludence protein 1 (ZO-1) and the tight junction protein occludin or the adherens junction protein alpha-catenin are unknown...
- Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctionsRaphaël Etournay
INSERM UMRS 587, Unité de Génétique des Déficits Sensoriels, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
J Cell Sci 120:2838-50. 2007....
- Down-regulation of survival signaling through MAPK and Akt in occludin-deficient mouse hepatocytes in vitroMasaki Murata
Department of Pathology, Sapporo Medical University School of Medicine, S1 W17 Sapporo 060 8556, Japan
Exp Cell Res 310:140-51. 2005..b>Occludin was the first integral membrane protein to be discovered, but it is not indispensable for the formation of TJ ..
- Potential role of MCP-1 in endothelial cell tight junction 'opening': signaling via Rho and Rho kinaseSvetlana M Stamatovic
Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA
J Cell Sci 116:4615-28. 2003..of actin cytoskeleton (stress fiber formation) and redistribution of tight junction proteins, ZO-1, ZO-2 occludin and claudin-5, from the Triton X-100-soluble to the Triton X-100-insoluble fractions...
- Differential expression and subcellular localization of claudin-7, -8, -12, -13, and -15 along the mouse intestineHiroki Fujita
Dept of Pathology, Sapporo Medical University School of Medicine, S1, W17, Sapporo 060 8556, Japan
J Histochem Cytochem 54:933-44. 2006..This manuscript contains online supplemental material available at http://www.jhc.org. Please visit this article online to view these materials...
- The Grainyhead-like epithelial transactivator Get-1/Grhl3 regulates epidermal terminal differentiation and interacts functionally with LMO4Zhengquan Yu
Departments of Medicine and Biological Chemistry, University of California, Irvine, CA 92697 4030, USA
Dev Biol 299:122-36. 2006..These findings indicate that the Get-1 and LMO4 genes interact functionally to regulate epidermal terminal differentiation...
- Distinct subdomain organization and molecular composition of a tight junction with adherens junction featuresFabio D Nunes
Laboratory of Cellular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 119:4819-27. 2006....
- Dicer is required for Sertoli cell function and survivalGwang Jin Kim
Institute of Human Genetics, University of Freiburg, Germany
Int J Dev Biol 54:867-75. 2010..Taken together, the results of this study show that Dicer is required for Sertoli cell function and survival and for spermatogenesis in mice...
- Differential effects of testosterone and TGF-β3 on endocytic vesicle-mediated protein trafficking events at the blood-testis barrierLinlin Su
Center for Biomedical Research, Population Council, New York, NY 10065, USA
Exp Cell Res 316:2945-60. 2010..Testosterone and TGF-β3 also induced the association between internalized occludin (a BTB integral membrane protein) and clathrin, as well as early endosome antigen-1 (EEA-1)...
- Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrierRana Al-Sadi
Department of Internal Medicine, University of New Mexico, Albuquerque, NM 87131 0001, USA
Am J Physiol Gastrointest Liver Physiol 300:G1054-64. 2011..The expression of occludin is markedly decreased in intestinal permeability disorders, including in Crohn's disease, ulcerative colitis, and ..
- Dynamic migration of γδ intraepithelial lymphocytes requires occludinKaren L Edelblum
Department of Pathology, University of Chicago, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 109:7097-102. 2012..As a result, each γδ IEL contacts multiple epithelial cells. Occludin is concentrated at sites of γδ IEL/epithelial interaction, where it forms a ring surrounding the γδ IEL...
- Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisitionG Youssef
Livingstone Skin Research Centre for Children, UCL Institute of Child Health, London WC1N 1EH, UK
Dev Biol 380:274-85. 2013..This was accompanied by a change of Claudin-1 localisation to the cell surface and interaction between Occludin and Claudin-1 which are thought to be required for tight junction formation...
- Phenotypical analysis of atypical PKCs in vivo function display a compensatory system at mouse embryonic day 7.5Sebastian Seidl
The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
PLoS ONE 8:e62756. 2013..Thus, much effort has been made during the last decades to characterize the functions of aPKCs using gene targeting approaches and depletion studies. However, little is known about the specific roles of each isoform in mouse development...
- beta-Catenin protects the epidermis from mechanical stressesSamriddha Ray
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
J Cell Biol 202:45-52. 2013..These data demonstrate that a complete understanding of the functions of cell adhesion proteins must take into account their roles in response to mechanical stresses. ..
- Crumbs3 is essential for proper epithelial development and viabilityEileen L Whiteman
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA
Mol Cell Biol 34:43-56. 2014..Taken together, our data indicate that Crumbs3 is crucial for epithelial morphogenesis and plays a role in linking the apical membrane to the underlying ezrin-containing cytoskeleton. ..
- Flightless I over-expression impairs skin barrier development, function and recovery following skin blisteringZlatko Kopecki
Centre for Regenerative Medicine, Mawson Institute, University of South Australia, Adelaide, Australia Women s and Children s Health Research Institute, Adelaide, Australia
J Pathol 232:541-52. 2014..These results therefore demonstrate an important role for Flii in the development and regulation of the epidermal barrier, which may contribute to the impaired healing and skin fragility of EB patients...
- Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: implications in intestinal permeability and susceptibility to colitisOh oka Kyoko
Department of Immunology, University of Yamanashi Faculty of Medicine, Yamanashi, Japan
PLoS ONE 9:e98016. 2014..Because intestinal permeability depends on tight junction (TJ) that regulates the epithelial paracellular pathway, this study investigated whether the circadian clock regulates the expression levels of TJ proteins in the intestine...
- Hypoxia-inducible factor/MAZ-dependent induction of caveolin-1 regulates colon permeability through suppression of occludin, leading to hypoxia-induced inflammationLiwei Xie
Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan, USA
Mol Cell Biol 34:3013-23. 2014..Hypoxic induction of CAV1 in the colon was essential for intestinal barrier integrity by regulating occludin expression...
- Keratin 76 is required for tight junction function and maintenance of the skin barrierTia DiTommaso
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
PLoS Genet 10:e1004706. 2014....
- Redox Regulation of Cell Contacts by Tricellulin and Occludin: Redox-Sensitive Cysteine Sites in Tricellulin Regulate Both Tri- and Bicellular Junctions in Tissue Barriers as Shown in Hypoxia and IschemiaJimmi Cording
1 Leibniz Institut für Molekulare Pharmakologie, Berlin, Germany
Antioxid Redox Signal 23:1035-49. 2015..TJ-associated marvel proteins (TAMPs; tricellulin, occludin, marvelD3) are thought to be relevant to regulation...
- Elucidating pathways of Toxoplasma gondii invasion in the gastrointestinal tract: involvement of the tight junction protein occludinCaroline M Weight
Gut Health and Food Safety Institute Strategic Programme, Norwich Research Park, Norwich, NR4 7UA, UK Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7UA, UK
Microbes Infect 17:698-709. 2015..During invasion, T. gondii co-localized with occludin. Reducing the levels of endogenous cellular occludin with specific small interfering RNAs significantly reduced ..
- Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal NeovascularizationXuwen Liu
Department of Ophthalmology and Visual Sciences, University of Michigan, Kellogg Eye Center, Ann Arbor, Michigan
Am J Pathol 186:2486-99. 2016b>Occludin is a transmembrane tight junction protein that contributes to diverse cellular functions, including control of barrier properties, cell migration, and proliferation...
- Milk secretion and ejection are impaired in the mammary gland of mice harboring a Cx43 mutant while expression and localization of tight and adherens junction proteins remain unchangedIsabelle Plante
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada
Biol Reprod 82:837-47. 2010..localization of Cx26 (Gjb2), Cx30 (Gjb6), Cx32 (Gjb1), E-cadherin, P-cadherin, beta-catenin, claudin-1, and occludin were all unaffected by the decreased levels of Cx43 at parturition, suggesting no cross-talk to other connexins ..
- Brain penetration of the OAB drug trospium chloride is not increased in aged miceJasmin Kranz
Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, Frankfurter Str 107, 35392, Giessen, Germany
World J Urol 31:219-24. 2013....
- The Hedgehog pathway promotes blood-brain barrier integrity and CNS immune quiescenceJorge Ivan Alvarez
Neuroimmunology Unit, Center of Excellence in Neuromics, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada
Science 334:1727-31. 2011..Overall, the Hh pathway provides a barrier-promoting effect and an endogenous anti-inflammatory balance to CNS-directed immune attacks, as occurs in multiple sclerosis...
- Two novel EGFP insertion alleles reveal unique aspects of Pax2 function in embryonic and adult kidneysAbdul Soofi
Dept of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
Dev Biol 365:241-50. 2012..These experiments provide new insight into the role of Pax2 protein levels in determining correct renal architecture and cell fate. These new Pax2 alleles are valuable genetic reagents for in vivo studies of urogenital development...
- Plasticity of button-like junctions in the endothelium of airway lymphatics in development and inflammationLi Chin Yao
Department of Anatomy, Cardiovascular Research Institute, and the Comprehensive Cancer Center, University of California, San Francisco, CA 94143, USA
Am J Pathol 180:2561-75. 2012..These findings demonstrate the plasticity of intercellular junctions in lymphatics during development and inflammation and show that button formation can be promoted by glucocorticoid receptor signaling in lymphatic endothelial cells...
- Reduced expression of epidermal growth factor receptor, E-cadherin, and occludin in the skin of flaky tail mice is due to filaggrin and loricrin deficienciesKozo Nakai
Department of Dermatology, Faculty of Medicine, Kagawa University, Japan
Am J Pathol 181:969-77. 2012..Herein, we report the decreased expression of epidermal growth factor receptor (EGFR), E-cadherin, occludin, and SIRT1 in the skin of Flg(ft) mice, compared with those in C57BL/6J mice...
- The Wilms tumor gene, Wt1, maintains testicular cord integrity by regulating the expression of Col4a1 and Col4a2Su Ren Chen
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Biol Reprod 88:56. 2013..Compared with control testes, the expression of CAMs (such as E-cadherin, N-cadherin, claudin11, occludin, beta-catenin, and ZO-1) was not obviously altered in Wt1(-/flox); Amh-Cre testes...
- Cell-junctional and cytoskeletal organization in mouse blastocysts lacking E-cadherinM Ohsugi
Max Planck Institut fur Immunbiologie, Freiburg, Germany
Dev Biol 185:261-71. 1997..against alpha-, beta-, or gamma-(plakoglobin)-catenin and junctional and cytoskeletal proteins including ZO-1 and occludin (tight junctions), desmoglein1 (desmosomes), connexin43 (gap junctions), and EndoA (cytokeratin intermediate ..
- Tight junction proteinsS Citi
Department of Molecular Biology, University of Geneva, Geneva, Switzerland
Biochim Biophys Acta 1448:1-11. 1998
- Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha cateninM Itoh
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
J Biol Chem 274:5981-6. 1999..Consistently, occludin as well as alpha catenin directly bound to N-ZO-2 as well as the NH2-terminal dlg-like portion of ZO-1 (N-ZO-1) ..
- Cellular immunolocalization of occludin during embryonic and postnatal development of the mouse testis and epididymisD G Cyr
Centre de Recherche en Santé Humaine, Institut National de la Recherche Scientifique Institut Armand Frappier, Universite du Quebec, Pointe Claire, Canada
Endocrinology 140:3815-25. 1999..In the present study, occludin, a constituent integral membrane protein of tight junctions, was localized by immunofluorescent confocal ..
- Evolutionary divergence of the mouse and human Lgn1/SMA repeat structuresJ D Growney
Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Genomics 64:62-81. 2000..Several, but not all, of these Naip loci are contained within the Lgn1 critical interval. We present a model for the origins of the mouse and human repetitive arrays from a common ancestral haplotype...
- Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junctionB Sheth
Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton, UK
Mech Dev 97:93-104. 2000..However, following assembly of the TJ transmembrane protein, occludin, from the early 32-cell stage when blastocoel formation begins, ZO-1alpha- and other TJ proteins (ZO-1alpha+ ..
- The murine gene, Traube, is essential for the growth of preimplantation embryosT Thomas
Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Am Fassberg 11, Goettingen, 37077, Germany
Dev Biol 227:324-42. 2000..This paucity of ribosomes together with the localization of TRB to the nucleoli, the site of ribosome synthesis, suggests that TRB is involved in the synthesis of ribosomes...
- Lack of pericytes leads to endothelial hyperplasia and abnormal vascular morphogenesisM Hellstrom
Department of Medical Biochemistry, Goteborg University, SE 405 30 Goteborg, Sweden
J Cell Biol 153:543-53. 2001....
- Protein kinase C regulates the phosphorylation and cellular localization of occludinA Y Andreeva
Forschungsinstitut fur Molekulare Pharmakologie, 13125 Berlin Buch and Charité, Humboldt Universitat Berlin, 13092 Berlin, Germany
J Biol Chem 276:38480-6. 2001b>Occludin is an integral membrane phosphoprotein specifically associated with tight junctions, contributing to the structure and function of this intercellular seal...
- Vascular abnormalities in mice lacking the endothelial gap junction proteins connexin37 and connexin40Alexander M Simon
Department of Physiology, University of Arizona, Tucson, 85724, USA
Dev Biol 251:206-20. 2002..These results provide evidence of a critical role for endothelial gap junction-mediated communication in the development and/or functional maintenance of segments of the mouse vasculature...
- Dynamic assembly of tight junction-associated proteins ZO-1, ZO-2, ZO-3 and occludin during mouse tooth developmentF J Unda
Departamento de Biologia Celular y Ciencias Morfologicas, Facultad de Medicina y Odontologia, Universidad del Pais Vasco Euskal Herriko Unibertsitatea, Leioa, Vizcaya, Spain
Histol Histopathol 18:27-38. 2003..we have studied the distribution pattern of the tight junction-associated proteins ZO-1, ZO-2, ZO-3 and occludin in the developing mouse tooth as a model...
- Role of connexin37 and connexin40 in vascular developmentAlexander M Simon
Department of Physiology, University of Arizona, Tucson, Arizona, USA
Cell Commun Adhes 10:379-85. 2003..In addition, Cx37 and Cx40 appear to be mutually dependent on each other for normal expression in vascular endothelium...
- Expression patterns of claudin family of tight junction membrane proteins in developing mouse submandibular glandAtsushi Hashizume
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
Dev Dyn 231:425-31. 2004..These results will provide a valuable resource for further investigation of tubulogenesis and physiological regulation of claudin-based tight junctions...
- E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctionsJudith A Tunggal
Center for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
EMBO J 24:1146-56. 2005..Surprisingly, our results indicate that E-cadherin is specifically required for tight junction, but not desmosome, formation and this appears to involve signalling rather than cell contact formation...
- Developmental changes in the expression of tight junction protein claudins in murine metanephroi and embryonic kidneysHiroshi Ohta
Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
J Vet Med Sci 68:149-55. 2006..chain reaction for the claudins examined, including claudins 1-4, 8, 10, 11, and 16, and the TJ proteins occludin and ZO-1...
- The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasisElisabeth Raschperger
Ludwig Institute for Cancer Research, Nobels v 3, SE 171 77 Stockholm, Sweden
Exp Cell Res 312:1566-80. 2006..the body cavities in adult mice, where it specifically co-localizes with the tight junction components ZO-1 and occludin. Notably, CAR could not be detected in endothelial cells of the vasculature, including brain capillaries...
- Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cellsMito Kanatsu-Shinohara
Department of Molecular Genetics, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan tshinoha2virus kyoto u ac jp
Proc Natl Acad Sci U S A 103:8018-23. 2006..Using this strategy, the efficiency of homologous recombination for the occludin gene locus was 1.7% using a nonisogenic DNA construct...
- Involvement of MAPKs in endostatin-mediated regulation of blood-retinal barrier functionMatthew Campbell
UCD School of Biomolecular Biomedical Sciences, Conway Institute, University College Dublin, Dublin, Ireland
Curr Eye Res 31:1033-45. 2006..Moreover, it was hypothesized that endostatin-induced occludin upregulation regulated VEGF165-mediated increases in endothelial cell permeability and involved activation of the ..
- Occludin-mediated premature senescence is a fail-safe mechanism against tumorigenesis in breast carcinoma cellsMakoto Osanai
Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan
Cancer Sci 98:1027-34. 2007We have previously demonstrated that epigenetic silencing of occludin, a tight junction-associated membrane protein, results in the acquisition of apoptotic resistance to various apoptogenic stimuli, causally contributing to the enhanced ..
- Coxsackievirus and adenovirus receptor is up-regulated in migratory germ cells during passage of the blood-testis barrierMomina Mirza
Ludwig Institute for Cancer Research, Stockholm Branch, Karolinska Institutet, Box 240, SE 17177, Stockholm, Sweden
Endocrinology 148:5459-69. 2007..Finally, an intermediate compartment occupied by CAR-positive, migrating germ cells and flanked by two occludin-containing junctions is identified...
- Production of knockout mice by gene targeting in multipotent germline stem cellsMasanori Takehashi
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
Dev Biol 312:344-52. 2007..Spermatozoa that were deficient in the occludin gene could be recovered from animals that were chimeric with mGS cells that underwent homologous recombination...
- Phosphorylation of claudin-5 and occludin by rho kinase in brain endothelial cellsMasaru Yamamoto
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
Am J Pathol 172:521-33. 2008..Posttranslational modifications of essential endothelial TJ proteins, occludin and claudin-5, contribute and possibly disrupt BBB integrity...
- ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degenerationZhihui Zhong
Center for Neurodegenerative and Vascular Brain Disorders and Department of Neurosurgery, University of Rochester Medical Center, Kornberg Medical Research Bldg, 601 Elmwood Avenue, Box 670, Rochester, New York 14642, USA
Nat Neurosci 11:420-2. 2008..disrupted the blood-spinal cord barrier in mice by reducing the levels of the tight junction proteins ZO-1, occludin and claudin-5 between endothelial cells...
- Cell-surface biotinylation to study endocytosis and recycling of occludinNoriyuki Nishimura
Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan
Methods Mol Biol 440:89-96. 2008..b>Occludin and claudins are distinct transmembrane proteins localized to the TJs...
- Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8Francisco Barrionuevo
Institute of Human Genetics and Anthropology, University of Freiburg, Breisacherstr 33, D 79106 Freiburg, Germany
Dev Biol 327:301-12. 2009..This study shows that testis cord differentiation is independent of Sox9, and that concerted Sox9 and Sox8 function in post E14.0 Sertoli cells is essential for the maintenance of testicular function...
- Wnt/beta-catenin signaling is required for CNS, but not non-CNS, angiogenesisRichard Daneman
Stanford University School of Medicine, Department of Developmental Biology, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 106:641-6. 2009..Taken together these experiments reveal an essential role for Wnt/beta-catenin signaling in driving CNS-specific angiogenesis and provide molecular evidence that angiogenesis and BBB formation are in part linked...
- Myc regulates embryonic vascular permeability and remodelingEniko Kokai
Institute of Physiological Chemistry, Ulm University, Ulm, Germany
Circ Res 104:1151-9. 2009..Thus, the net outcome of an excess of vascular endothelial growth factor-A and angiopoietin-2 in the face of an elevated cellular turnover appears to be a defect in vascular integrity...
- CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarityLarissa Lozovatsky
Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA
Mol Biol Cell 20:4489-99. 2009..Thus, CASK expression and the appropriate localization of DLG1 are not essential for either epithelial polarity or intestinal homeostasis in vivo...
- Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failureFeng Chen
Department of Transplantation, Mayo Clinic, Jacksonville, FL 32224, USA
Hepatology 50:1914-23. 2009..Endogenous overexpression of MMP-9 in brain ECs resulted in significant degradation of the TJ proteins occludin and claudin-5...
- Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and RaptorPreethi Vijayaraj
Abteilung für Zellbiochemie, Institut für Biochemie und Molekularbiologie and 2 Bonner Forum Biomedizin, Universitat Bonn, 53115 Bonn, Germany
J Cell Biol 187:175-84. 2009..Our findings demonstrate a novel keratin function upstream of mTOR signaling via GLUT localization and have implications for pathomechanisms and therapy approaches for keratin disorders and the analysis of other gene families...
- An essential role for RPE-derived soluble VEGF in the maintenance of the choriocapillarisMagali Saint-Geniez
Department of Ophthalmology, Schepens Eye Research Institute, Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 106:18751-6. 2009..These changes are reminiscent of geographic atrophy (GA) and point to a role for RPE-derived VEGF in the maintenance of the choriocapillaris...
- Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signatureHarold Kim
Sunnybrook Research Institute University of Toronto 2075 Bayview Avenue Toronto, Ontario M4N 3M5, Canada
BMC Dev Biol 10:72. 2010..While the in vitro data suggest that gene reprogramming occurs upon Prox1 expression, it is not clear if this is a direct result of Prox1 in vascular endothelial cells in vivo...
- Differential expression patterns of the tight junction-associated proteins occludin and claudins in secretory and mature ameloblasts in mouse incisorMasaki Hata
Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama, 700 8525, Japan
Med Mol Morphol 43:102-6. 2010..primarily as a barrier against paracellular transport between epithelial cells and are composed mainly of occludin (OLD) and claudins (CLDs). The CLD family consists of 24 members that show tissue- or cell-specific expression...
- Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyriaMary C O'Driscoll
University of Manchester, Manchester Academic Health Science Centre, Central Manchester Foundation Trust University Hospitals, UK
Am J Hum Genet 87:354-64. 2010..The OCLN gene encodes occludin, an integral component of tight junctions...
- Pericytes are required for blood-brain barrier integrity during embryogenesisRichard Daneman
UCSF Department of Anatomy, 513 Parnassus Avenue, HSW1301, San Francisco, California 94143 0452, USA
Nature 468:562-6. 2010..These data indicate that pericyte-endothelial cell interactions are critical to regulate the BBB during development, and disruption of these interactions may lead to BBB dysfunction and neuroinflammation during CNS injury and disease...
- Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in miceKen Kobayashi
Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjyuku ku, Tokyo, 160 8582, Japan
Cell Tissue Res 342:307-16. 2010....
- Abnormal epithelial cell polarity and ectopic epidermal growth factor receptor (EGFR) expression induced in Emx2 KO embryonic gonadsMasatomo Kusaka
Division for Sex Differentiation, Center for Transgenic Animals and Plants, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki 444 8787, Japan
Endocrinology 151:5893-904. 2010..Our results strongly suggest that Emx2 is required for regulation of tight junction assembly and allowing migration of the gonadal epithelia to the mesenchyme, which are possibly mediated by suppression of Egfr expression...
- The transcription factor grainyhead-like 2 regulates the molecular composition of the epithelial apical junctional complexMax Werth
Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13125 Berlin, Germany
Development 137:3835-45. 2010..Together, our data provide evidence that Grhl2 acts as a target gene-associated transcriptional activator of apical junctional complex components and, thereby, crucially participates in epithelial differentiation...
- Claudin-2 forms homodimers and is a component of a high molecular weight protein complexChristina M Van Itallie
Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
J Biol Chem 286:3442-50. 2011..identified a relatively discrete high molecular weight complex containing at least claudin-1, claudin-2, and occludin; the difference in the protein complex sizes between cultured cells and tissues may reflect differences in tight ..
- Expression of coxsackievirus and adenovirus receptor isoforms in developing mouse bladder uroepitheliumMyung Chan Gye
Department of Life Science, Hanyang University College of Natural Sciences, Seoul Korea
Urology 77:1009.e9-1009.e18. 2011..In the present study, we examined the developmental changes in the expression of coxsackievirus and adenovirus receptor (CAR) isoforms in mouse bladder uroepithelium...
- Impact of intra- and interspecies variation of occludin on its function as coreceptor for authentic hepatitis C virus particlesSandra Ciesek
Medizinische Hochschule Hannover, Klinik fur Gastroenterologie, Hepatologie und Endokrinologie, Carl Neuberg Str 1, 30625 Hannover, Germany
J Virol 85:7613-21. 2011..The tight junction component occludin (OCLN) is essential for HCV entry into host cells, and the differences between human and murine OCLN are thought ..
- EMILIN1-α4/α9 integrin interaction inhibits dermal fibroblast and keratinocyte proliferationCarla Danussi
Division of Experimental Oncology 2, Centro di Riferimento Oncologico, Istituto di Ricovero e Cura a Carattere Scientifico, National Cancer Institute, 33081 Aviano, Italy
J Cell Biol 195:131-45. 2011..In addition, EMILIN1 is identified as a novel ligand for keratinocyte α9β1 integrin, suggesting prospective roles for this receptor-ligand pair in skin homeostasis...
- Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrierSeiji Takashima
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
Cell Stem Cell 9:463-75. 2011..Short hairpin RNA (shRNA) -mediated suppression of claudin3, which was downregulated by Rac inhibition, reduced the SSC homing efficiency. Thus, Rac1 is a critical regulator of SSC homing and proliferation...
- Schwann cell-derived Desert hedgehog controls the development of peripheral nerve sheathsE Parmantier
Department of Anatomy and Developmental Biology, University College London, United Kingdom
Neuron 23:713-24. 1999..They also show that Schwann cell-derived signals can act as important regulators of nerve development...
- Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cellsK Morita
Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
J Cell Biol 147:185-94. 1999..e., the extracellular face-associated TJs. These findings indicated that claudin-5/TMVCF is an endothelial cell-specific component of TJ strands...
- Post-translational control of occludin membrane assembly in mouse trophectoderm: a mechanism to regulate timing of tight junction biogenesis and blastocyst formationB Sheth
Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
Development 127:831-40. 2000..mechanisms regulating the timing of blastocyst formation, we have examined the dynamics of expression of occludin, an integral membrane protein of the TJ...
- High-resolution genetic and physical map of the Lgn1 interval in C57BL/6J implicates Naip2 or Naip5 in Legionella pneumophila pathogenesisJ D Growney
Harvard Medical School Department of Genetics, Boston, Massachusetts 02115, USA
Genome Res 10:1158-71. 2000..Our genetic map suggests the presence of two hotspots of recombination within the Naip array, indicating that the 3' portion of Naip may be involved in the genomic instability at this locus...
- Molecular architecture of tight junctions of periderm differs from that of the maculae occludentes of epidermisKazumasa Morita
Department of Dermatology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
J Invest Dermatol 118:1073-9. 2002b>Occludin and claudins are tetraspan-transmembrane proteins in tight junctions. Maculae occludentes, which are less-developed tight junctions, occur in the granular cell layer of the epidermis...
- Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing mouseKyoji Takekuni
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
J Biol Chem 278:5497-500. 2003..These results indicate that nectin-1 and -3 are involved in the localization of PAR-3 at AJs in the neuroepithelial cells of the embryonic telencephalon...
- Identification of PKCzetaII: an endogenous inhibitor of cell polarityScott J Parkinson
Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, UK
EMBO J 23:77-88. 2004..The data demonstrate a regulatory role for PKCzetaII in the maintenance of cell transformation and the development of cell polarity...
- Role of occludin, a tight junction protein, in blastocoel formation, and in the paracellular permeability and differentiation of trophectoderm in preimplantation mouse embryosJinmee Kim
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133 791, Korea
Mol Cells 17:248-54. 2004..We examined the effect of occludin antibody on blastocoel formation, blastocyst permeability, and expression of H19 and Oct-4, markers of TE ..
- Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrierHeping Xu
Department of Ophthalmology, School of Medicine, University of Aberdeen, Aberdeen, Scotland, United Kingdom
Invest Ophthalmol Vis Sci 46:2487-94. 2005..The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial...
- Abnormal development of urogenital organs in Dlgh1-deficient miceAkiko Iizuka-Kogo
Department of Anatomy I, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
Development 134:1799-807. 2007..Our findings might contribute to a better understanding of such abnormalities...
- REGULATION OF HUMAN GAMMA AND BETA GLOBIN GENESJoyce Lloyd; Fiscal Year: 2003..These results will predict the proteins involved in early beta-globin gene suppression. ..
- Role of KLF2 in erythropoiesis and globin expressionJoyce A Lloyd; Fiscal Year: 2010..Our long-term goal is to use KLF2 in strategies to increase embryonic/fetal beta-globin, to treat patients with beta-hemoglobinopathies. ..
- Gap Junction Mediated Signaling and Vascular ResponsesAlexander M Simon; Fiscal Year: 2010..Finally, these studies could also lead to new approaches to control vasculogenesis and angiogenesis in other clinically important settings, such as anti-angiogenesis treatments designed to fight cancer. ..