Nos3

Summary

Gene Symbol: Nos3
Description: nitric oxide synthase 3, endothelial cell
Alias: 2310065A03Rik, Nos-3, eNOS, ecNOS, nitric oxide synthase, endothelial, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, endothelial nitric oxide synthase
Species: mouse

Top Publications

  1. doi The adverse cardiopulmonary phenotype of caveolin-1 deficient mice is mediated by a dysfunctional endothelium
    Carsten Wunderlich
    University of Technology Dresden, Department of Medicine and Cardiology, Medical Clinic, Fetscherstr 76, Dresden 01307, Germany
    J Mol Cell Cardiol 44:938-47. 2008
  2. pmc Follistatin-like 1, a secreted muscle protein, promotes endothelial cell function and revascularization in ischemic tissue through a nitric-oxide synthase-dependent mechanism
    Noriyuki Ouchi
    Molecular Cardiology Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 283:32802-11. 2008
  3. doi Overexpression of eNOS prevents the development of renovascular hypertension in mice
    Agata L Gava
    Laboratory of Transgenes and Cardiovascular Control, Physiological Sciences Graduate Program, Health Sciences Center, Federal University of Espirito Santo, Avenida Marechal Campos 1468, Vitoria, ES 29043 900, Brazil
    Can J Physiol Pharmacol 86:458-64. 2008
  4. pmc Liver receptor homolog 1 is essential for ovulation
    Rajesha Duggavathi
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 67404 Illkirch, France
    Genes Dev 22:1871-6. 2008
  5. doi Nitric oxide synthases are crucially involved in the development of the severe cardiomyopathy of caveolin-1 knockout mice
    Carsten Wunderlich
    University of Technology Dresden, Department of Medicine and Cardiology, Medical Clinic, Fetscherstr 76, Dresden 01307, Germany
    Biochem Biophys Res Commun 377:769-74. 2008
  6. ncbi Direct interaction of endothelial nitric-oxide synthase and caveolin-1 inhibits synthase activity
    H Ju
    Vascular Biology Center, Department of Pediatrics, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 272:18522-5. 1997
  7. pmc Disrupted cardiac development but normal hematopoiesis in mice deficient in the second CXCL12/SDF-1 receptor, CXCR7
    Frederic Sierro
    Department of Immunology and Inflammation, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst NSW 2010, Australia
    Proc Natl Acad Sci U S A 104:14759-64. 2007
  8. pmc Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1 alpha
    Katherine A Gallagher
    Department of Surgery, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104, USA
    J Clin Invest 117:1249-59. 2007
  9. ncbi Insulin resistance, hyperlipidemia, and hypertension in mice lacking endothelial nitric oxide synthase
    H Duplain
    Department of Internal Medicine, Division of Hypertension and Vascular Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Circulation 104:342-5. 2001
  10. pmc A noninhibitory mutant of the caveolin-1 scaffolding domain enhances eNOS-derived NO synthesis and vasodilation in mice
    Pascal Bernatchez
    Providence Heart and Lung Institute, St Paul s Hospital, James Hogg Research Centre, 1081 Burrard St, Room 166, Vancouver BC Canada, V6Z 1Y6
    J Clin Invest 121:3747-55. 2011

Research Grants

Detail Information

Publications202 found, 100 shown here

  1. doi The adverse cardiopulmonary phenotype of caveolin-1 deficient mice is mediated by a dysfunctional endothelium
    Carsten Wunderlich
    University of Technology Dresden, Department of Medicine and Cardiology, Medical Clinic, Fetscherstr 76, Dresden 01307, Germany
    J Mol Cell Cardiol 44:938-47. 2008
    ..A plethora of studies support a role of cav-1 as a negative regulator of endothelial nitric oxide synthase (eNOS). Accordingly, constitutive eNOS hyperactivation was observed in cav-1(-/-)...
  2. pmc Follistatin-like 1, a secreted muscle protein, promotes endothelial cell function and revascularization in ischemic tissue through a nitric-oxide synthase-dependent mechanism
    Noriyuki Ouchi
    Molecular Cardiology Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 283:32802-11. 2008
    ..density in the ischemic hind limbs of wild-type mice, and this was associated with an increase in endothelial nitric oxide synthase (eNOS) phosphorylation at residue Ser-1179...
  3. doi Overexpression of eNOS prevents the development of renovascular hypertension in mice
    Agata L Gava
    Laboratory of Transgenes and Cardiovascular Control, Physiological Sciences Graduate Program, Health Sciences Center, Federal University of Espirito Santo, Avenida Marechal Campos 1468, Vitoria, ES 29043 900, Brazil
    Can J Physiol Pharmacol 86:458-64. 2008
    ..In the present study we investigated the effects of endothelial nitric oxide synthase (eNOS) overexpression on renovascular hypertension...
  4. pmc Liver receptor homolog 1 is essential for ovulation
    Rajesha Duggavathi
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 67404 Illkirch, France
    Genes Dev 22:1871-6. 2008
    ..These results demonstrate that Lrh1 is a regulator of multiple mechanisms essential for maturation of ovarian follicles and for ovulation. Lrh1 is therefore a key modulator of female fertility and a potential target for contraception...
  5. doi Nitric oxide synthases are crucially involved in the development of the severe cardiomyopathy of caveolin-1 knockout mice
    Carsten Wunderlich
    University of Technology Dresden, Department of Medicine and Cardiology, Medical Clinic, Fetscherstr 76, Dresden 01307, Germany
    Biochem Biophys Res Commun 377:769-74. 2008
    ..Mounting evidence indicates that cav-1 acts as a negative regulator of endothelial nitric oxide synthase resulting in a constitutive hyperactivation of the nitric oxide (NO)-pathway in cav-1 knockout mice (..
  6. ncbi Direct interaction of endothelial nitric-oxide synthase and caveolin-1 inhibits synthase activity
    H Ju
    Vascular Biology Center, Department of Pediatrics, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 272:18522-5. 1997
    Endothelial nitric-oxide synthase (eNOS) and caveolin-1 are associated within endothelial plasmalemmal caveolae...
  7. pmc Disrupted cardiac development but normal hematopoiesis in mice deficient in the second CXCL12/SDF-1 receptor, CXCR7
    Frederic Sierro
    Department of Immunology and Inflammation, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst NSW 2010, Australia
    Proc Natl Acad Sci U S A 104:14759-64. 2007
    ..Our results reveal a specialized role for CXCR7 in endothelial biology and valve development and highlight the distinct developmental role of evolutionary conserved chemokine receptors such as CXCR7 and CXCR4...
  8. pmc Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1 alpha
    Katherine A Gallagher
    Department of Surgery, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104, USA
    J Clin Invest 117:1249-59. 2007
    ..Since mobilization of BM EPCs occurs via eNOS activation, we hypothesized that eNOS activation is impaired in diabetes, which results in reduced EPC mobilization...
  9. ncbi Insulin resistance, hyperlipidemia, and hypertension in mice lacking endothelial nitric oxide synthase
    H Duplain
    Department of Internal Medicine, Division of Hypertension and Vascular Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Circulation 104:342-5. 2001
    Insulin resistance and arterial hypertension are related, but the underlying mechanism is unknown. Endothelial nitric oxide synthase (eNOS) is expressed in skeletal muscle, where it may govern metabolic processes, and in the vascular ..
  10. pmc A noninhibitory mutant of the caveolin-1 scaffolding domain enhances eNOS-derived NO synthesis and vasodilation in mice
    Pascal Bernatchez
    Providence Heart and Lung Institute, St Paul s Hospital, James Hogg Research Centre, 1081 Burrard St, Room 166, Vancouver BC Canada, V6Z 1Y6
    J Clin Invest 121:3747-55. 2011
    Aberrant regulation of eNOS and associated NO release are directly linked with various vascular diseases. Caveolin-1 (Cav-1), the main coat protein of caveolae, is highly expressed in endothelial cells...
  11. doi Diabetic eNOS knockout mice develop distinct macro- and microvascular complications
    Sumathy Mohan
    Department of Pathology, The University of Texas Health Science Center, San Antonio, TX 78229, USA
    Lab Invest 88:515-28. 2008
    Functional consequences of impaired endothelial nitric oxide synthase (eNOS) activity causing organ-specific abnormalities on a diabetic setting are not completely understood...
  12. pmc Endothelial nitric oxide synthase deficiency produces accelerated nephropathy in diabetic mice
    Hui John Zhao
    Department of Medicine, Division of Nephrology and Hypertension, Vanderbilt University Medical Center, C 3121 MCN, Nashville, TN 37232, USA
    J Am Soc Nephrol 17:2664-9. 2006
    Functionally significant polymorphisms in endothelial nitric oxide synthase (eNOS) and reduced vascular eNOS activity have been associated with increased human diabetic nephropathy (DN), but the pathogenic role of eNOS deficiency in the ..
  13. ncbi Hyperglycaemia-induced superoxide production decreases eNOS expression via AP-1 activation in aortic endothelial cells
    S Srinivasan
    Division of Endocrinology and Metabolism, Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Diabetologia 47:1727-34. 2004
    ..The aim of this study was to investigate the regulation of endothelial nitric oxide synthase (eNOS) activity by acute and chronic elevated glucose.
  14. ncbi Deficient platelet-derived nitric oxide and enhanced hemostasis in mice lacking the NOSIII gene
    J E Freedman
    Department of Pharmacology, Georgetown University Medical Center, Washington, DC 20007, USA
    Circ Res 84:1416-21. 1999
    b>Endothelial nitric oxide synthase (eNOS) has been identified in human platelets...
  15. pmc Trans-arachidonic acids generated during nitrative stress induce a thrombospondin-1-dependent microvascular degeneration
    Elsa Kermorvant-Duchemin
    Department of Pediatrics, Ophthalmology and Pharmacology, Research Center, Hopital Ste Justine, 3175 Côte de Ste Catherine, Montreal, Quebec, H3T1C5, Canada
    Nat Med 11:1339-45. 2005
    ....
  16. ncbi Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal
    Cleber E Teixeira
    Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Sao Paulo, Brazil
    J Pharmacol Exp Ther 322:1093-102. 2007
    ..with nitric oxide (NO) in the corpus cavernosum (CC) from wild-type (WT), endothelial nitric-oxide synthase (eNOS)(-/-), and neuronal (n)NOS(-/-) mice...
  17. pmc Endothelial injury due to eNOS deficiency accelerates the progression of chronic renal disease in the mouse
    Takahiro Nakayama
    Division of Nephrology, Univ of Colorado Denver, P O Box C281, Aurora, CO 80045, USA
    Am J Physiol Renal Physiol 296:F317-27. 2009
    The vascular endothelium expresses endothelial nitric oxide synthase (eNOS) that generates nitric oxide (NO) to help maintain vascular integrity due to its anti-inflammatory, antiproliferative, and antithrombogenic effects...
  18. pmc Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: roles of endothelial nitric oxide synthase and endothelial progenitor cells
    Jinglian Yan
    Department of Surgery, University of Massachusetts Medical School, Worcester, Mass 01655, USA
    J Vasc Surg 50:1412-22. 2009
    ..We tested the hypothesis that type 2 diabetic mice have a greater reduction in endothelial nitric oxide synthase (eNOS) expression, a greater increase in oxidative stress, and reduced arteriogenesis and ..
  19. pmc Platelet aggregation responses are critically regulated in vivo by endogenous nitric oxide but not by endothelial nitric oxide synthase
    C Tymvios
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, London, UK
    Br J Pharmacol 158:1735-42. 2009
    ..In this paper we have investigated the roles of endogenous NO and NOS-3 in regulating platelet function in vivo and determined the functional contribution made by platelet-derived NO...
  20. pmc Caveolin-1 deficiency dampens Toll-like receptor 4 signaling through eNOS activation
    Muhammad K Mirza
    Department of Pharmacology, University of Illinois College of Medicine, Chicago, IL 60612, USA
    Am J Pathol 176:2344-51. 2010
    ..Here, using double mutant mice with genetic deletions of Cav1 and NOS3, we show that chronic endothelial nitric oxide synthase (eNOS) activation secondary to loss of Cav1 serves a crucial immunomodulatory function through ..
  21. ncbi Overexpression of arginase in the aged mouse penis impairs erectile function and decreases eNOS activity: influence of in vivo gene therapy of anti-arginase
    Trinity J Bivalacqua
    Brady Urological Institute, Johns Hopkins Hospital, Baltimore, MD, USA
    Am J Physiol Heart Circ Physiol 292:H1340-51. 2007
    ..In addition, endothelial NOS (NOS3) protein abundance was greater in aged versus young penile endothelial cells, whereas NOS activity and ..
  22. pmc Caveolin-1-eNOS signaling promotes p190RhoGAP-A nitration and endothelial permeability
    M Rizwan Siddiqui
    Department of Pharmacology, University of Illinois College of Medicine, Chicago, IL 60612, USA
    J Cell Biol 193:841-50. 2011
    ..cells isolated from Cav-1(-/-) mice, we show that Cav-1 deficiency induced the activation of endothelial nitric oxide synthase (eNOS) and the generation of nitric oxide (NO) and peroxynitrite...
  23. pmc Functional interplay between endothelial nitric oxide synthase and membrane type 1 matrix metalloproteinase in migrating endothelial cells
    Laura Genís
    Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain
    Blood 110:2916-23. 2007
    ..Endothelial NO synthase (eNOS) colocalizes with MT1-MMP at motility-associated structures in migratory human endothelial cells (ECs); moreover, ..
  24. ncbi Investigation of vascular responses in endothelial nitric oxide synthase/cyclooxygenase-1 double-knockout mice: key role for endothelium-derived hyperpolarizing factor in the regulation of blood pressure in vivo
    Ramona S Scotland
    Wolfson Institute for Biomedical Research, University College London, London, United Kingdom
    Circulation 111:796-803. 2005
    ..relaxation, we have generated mice in which the enzymes required for endothelial NO and PGI2 production, endothelial NO synthase (eNOS) and cyclooxygenase-1 (COX-1), respectively, have been disrupted (eNOS-/- and COX-1-/- mice).
  25. ncbi Lack of endothelial nitric oxide synthase promotes endothelin-induced hypertension: lessons from endothelin-1 transgenic/endothelial nitric oxide synthase knockout mice
    Thomas Quaschning
    Department of Nephrology, University Hospital of Freiburg, Freiburg, Germany
    J Am Soc Nephrol 18:730-40. 2007
    ..of the endothelial counterpart of ET-1, nitric oxide (NO), which is produced by the endothelial NO synthase (eNOS)...
  26. pmc Lack of endothelial nitric oxide synthase aggravates murine accelerated anti-glomerular basement membrane glomerulonephritis
    P Heeringa
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7525, USA
    Am J Pathol 156:879-88. 2000
    Nitric oxide (NO) radicals generated by endothelial nitric oxide synthase (eNOS) are involved in the regulation of vascular tone...
  27. ncbi NFkappaB and AP-1 differentially contribute to the induction of Mn-SOD and eNOS during the development of oxidant tolerance
    Tao Rui
    Vascular Cell Biology Laboratory, Centre for Critical Illness Research, Lawson Health Research Institute and Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada
    FASEB J 19:1908-10. 2005
    ..Although NF-kappaB and AP-1 (nuclear signaling) and Mn-SOD and eNOS (effector enzymes) have been implicated in the development oxidant tolerance, the precise relationship between the ..
  28. ncbi Mitochondrial arginase II constrains endothelial NOS-3 activity
    Hyun Kyo Lim
    Department of Anesthesiology, Johns Hopkins Hospital, The Johns Hopkins Medical Institutions, 600 N Wolfe St, Baltimore, MD 21287, USA
    Am J Physiol Heart Circ Physiol 293:H3317-24. 2007
    ..These data suggest that arginase II is predominantly confined to the mitochondria and that this mitochondrial arginase II regulates NO production, vascular endothelial function, and vascular stiffness by modulating NOS-3 activity...
  29. ncbi Over-expression of angiotensin II type 2 receptor (agtr2) reduces atherogenesis and modulates LOX-1, endothelial nitric oxide synthase and heme-oxygenase-1 expression
    Changping Hu
    Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences and VA Medical Center, Little Rock, AR 72205, United States
    Atherosclerosis 199:288-94. 2008
    ..Expression of endothelial nitric oxide synthase (eNOS) and heme-oxygenase-1 (HO-1) was decreased and that of the lectin-like oxidized-LDL receptor (..
  30. pmc Caveolin-1 ablation reduces the adverse cardiovascular effects of N-omega-nitro-L-arginine methyl ester and angiotensin II
    Luminita H Pojoga
    Brigham and Women s Hospital Harvard Medical School, Department of Endocrinology, Diabetes, and Hypertension, 221 Longwood Avenue, Boston, Massachusetts 02115, USA
    Endocrinology 151:1236-46. 2010
    ..cav-1) is the main component of caveolae; cav-1 modulates enzymes and receptors, such as the endothelial nitric oxide synthase and the angiotensin II (AngII) type 1 receptor...
  31. pmc Platelet-endothelial cell adhesion molecule-1 regulates endothelial NO synthase activity and localization through signal transducers and activators of transcription 3-dependent NOSTRIN expression
    Margaret E McCormick
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Arterioscler Thromb Vasc Biol 31:643-9. 2011
    NO produced by the endothelial NO synthase (eNOS) is an important regulator of cardiovascular physiological and pathological features. eNOS is activated by numerous stimuli, and its activity is tightly regulated...
  32. doi Molecular mechanisms of activation of endothelial nitric oxide synthase mediated by transient receptor potential vanilloid type 1
    Li Chieh Ching
    Department of Physiology, National Yang Ming University, Taipei, Taiwan
    Cardiovasc Res 91:492-501. 2011
    ..underlying the role of transient receptor potential vanilloid type 1 (TRPV1), a Ca(2+)-permeable non-selective cation channel, in the activation of endothelial nitric oxide (NO) synthase (eNOS) in endothelial cells (ECs) and mice.
  33. doi Activation of transient receptor potential vanilloid 1 by dietary capsaicin delays the onset of stroke in stroke-prone spontaneously hypertensive rats
    Xingsen Xu
    Department of Hypertension and Endocrinology, Daping Hospital, Third Military Medical University, Chongqing 400042, China
    Stroke 42:3245-51. 2011
    Previous studies show that endothelial nitric oxide synthase (eNOS) plays a prominent role in maintaining cerebral blood flow and preventing stroke. Capsaicin in hot pepper can increase the phosphorylation of eNOS in endothelial cells...
  34. ncbi [1,25(OH)(2)D(3) influences endothelial cell proliferation, apoptosis and endothelial nitric oxide synthase expression of aorta in apolipoprotein E-deficient mice]
    Wei Xiang
    Department of Pediatrics, Hainan Provincial People s Hospital, Haikou, China
    Zhonghua Er Ke Za Zhi 49:829-33. 2011
    To study possible influences of 1,25(OH)(2)D(3) on endothelial cell proliferation, apoptosis and endothelial nitric oxide synthase (eNOS) expression of aorta in apolipoprotein E-deficient (apoE(-/-)) mice and to explore the relationship ..
  35. pmc Uncoupling of eNOS causes superoxide anion production and impairs NO signaling in the cerebral microvessels of hph-1 mice
    Anantha Vijay R Santhanam
    Departments of Anesthesiology and Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic College of Medicine, Rochester, MN, USA
    J Neurochem 122:1211-8. 2012
    ..to test the hypothesis that the loss of tetrahydrobiopterin (BH(4)) in cerebral microvessels causes endothelial nitric oxide synthase (eNOS) uncoupling, resulting in increased superoxide anion production and inhibition of endothelial ..
  36. pmc Endothelin-1 inhibits thick ascending limb transport via Akt-stimulated nitric oxide production
    Marcela Herrera
    Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Michigan 48202, USA
    J Biol Chem 284:1454-60. 2009
    ..NO production by activating phosphatidylinositol 3-kinase (PI3K), stimulating Akt activity, and phosphorylating NOS3 at Ser1177. This enhances NO production and inhibits sodium transport...
  37. pmc Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids
    Florian P Limbourg
    Cardiovascular Division, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 110:1729-38. 2002
    ..20 mg/kg intraperitoneally), given within 2 hours of transient cerebral ischemia, acutely increased endothelial nitric oxide synthase (eNOS) activity, augmented regional cerebral blood flow (CBF) by 40% to 50%, and reduced cerebral ..
  38. ncbi Attenuation of angiotensin II signaling recouples eNOS and inhibits nonendothelial NOX activity in diabetic mice
    Jeong Ho Oak
    Division of Molecular Medicine, Department of Anesthesiology, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, 650 Charles E Young Dr South, Suite BH550 CHS, Los Angeles, CA 90095, USA
    Diabetes 56:118-26. 2007
    ..We recently reported that in aortic endothelial cells, Ang II induces endothelial nitric oxide synthase (eNOS) uncoupling to produce superoxide (O(2)*(-)) rather than nitric oxide (NO*), upon loss of the ..
  39. pmc Reduced NO-cGMP signaling contributes to vascular inflammation and insulin resistance induced by high-fat feeding
    Norma O Rizzo
    Department of Medicine, University of Washington, Seattle, WA 98109, USA
    Arterioscler Thromb Vasc Biol 30:758-65. 2010
    ..We sought to determine whether reduced NO-cGMP signaling contributes to the deleterious effects of DIO on the vasculature and, if so, whether these effects can be blocked by increased vascular NO-cGMP signaling...
  40. ncbi Leukocyte-endothelial cell interactions in nitric oxide synthase-deficient mice
    D J Lefer
    Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130, USA
    Am J Physiol 276:H1943-50. 1999
    ..mesentery of wild-type, inducible NOS (iNOS)-deficient (-/-), neuronal NOS (nNOS) -/-, and endothelial cell NOS (ecNOS) -/- mice to further our understanding of NO and leukocyte function...
  41. pmc Cutting edge: apoptosis-regulating signal kinase 1 is required for reactive oxygen species-mediated activation of IFN regulatory factor 3 by lipopolysaccharide
    Edward Chiang
    Division of Biological Sciences and University of California San Diego Cancer Center, University of California San Diego, La Jolla, CA 92093, USA
    J Immunol 176:5720-4. 2006
    ..ASK1 kinase activity proved essential for IRF3-mediated ISG induction by LPS. Thus, our results presented here suggest a novel role for ROS and ASK1 in the innate immune response as signaling intermediates in the IRF3 activation pathway...
  42. pmc Protective effects of PARP-1 knockout on dyslipidemia-induced autonomic and vascular dysfunction in ApoE mice: effects on eNOS and oxidative stress
    Chetan P Hans
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America
    PLoS ONE 4:e7430. 2009
    ..of PARP-1 gene deletion against dyslipidemia-induced endothelial dysfunction was associated with preservation of eNOS activity...
  43. pmc Elevated blood pressures in mice lacking endothelial nitric oxide synthase
    E G Shesely
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill 27599, USA
    Proc Natl Acad Sci U S A 93:13176-81. 1996
    Nitric oxide produced in endothelial cells affects vascular tone. To investigate the role of endothelial nitric oxide synthase (eNOS) in blood pressure regulation, we have generated mice heterozygous (+/-) or homozygous (-/-) for ..
  44. ncbi Long-term potentiation is reduced in mice that are doubly mutant in endothelial and neuronal nitric oxide synthase
    H Son
    Howard Hughes Medical Institute and Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA
    Cell 87:1015-23. 1996
    ..LTP was also normal in mice with a targeted mutation in endothelial NOS (eNOS-), but LTP in stratum radiatum of CA1 was significantly reduced in doubly mutant mice (nNOS-/eNOS-)...
  45. ncbi NO synthase-II is transiently expressed in embryonic mouse olfactory receptor neurons
    S Arnhold
    Department of Anatomy, Universitat zu Koln, Germany
    Neurosci Lett 229:165-8. 1997
    ..This transient expression of NOS-II implies a role for the differentiation of early olfactory receptor neurons and synaptic plasticity...
  46. pmc Nitric oxide synthases: which, where, how, and why?
    T Michel
    Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 100:2146-52. 1997
  47. pmc Cellular and molecular mechanisms of endothelial cell dysfunction
    D G Harrison
    Emory University, Department of Internal Medicine and the Atlanta Veterans Administration Medical Center, Atlanta, Georgia 30322, USA
    J Clin Invest 100:2153-7. 1997
  48. ncbi Responses of carotid artery in mice deficient in expression of the gene for endothelial NO synthase
    F M Faraci
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
    Am J Physiol 274:H564-70. 1998
    ..are impaired and responses to NO are enhanced in carotid artery from mice made deficient in endothelial nitric oxide synthase (eNOS) by gene targeting (eNOS-deficient mice)...
  49. pmc Contribution of nitric oxide synthases 1, 2, and 3 to airway hyperresponsiveness and inflammation in a murine model of asthma
    G T De Sanctis
    Pulmonary and Critical Care Divisions, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Exp Med 189:1621-30. 1999
    ..OVA/OVA endothelial (NOS3)-deficient mice were significantly more responsive to methacholine challenge compared with similarly treated NOS1 ..
  50. ncbi Protein expression, vascular reactivity and soluble guanylate cyclase activity in mice lacking the endothelial cell nitric oxide synthase: contributions of NOS isoforms to blood pressure and heart rate control
    G Kojda
    Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA
    Cardiovasc Res 42:206-13. 1999
    Both disruption of the endothelial nitric oxide synthase (eNOS) gene and pharmacological inhibition of the NOS produce modest hypertension...
  51. ncbi Vasodilator mechanisms in the coronary circulation of endothelial nitric oxide synthase-deficient mice
    K G Lamping
    Departments of Internal Medicine and Pharmacology, The Cardiovascular Center, University of Iowa, and Veterans Affairs Medical Center, Iowa City, Iowa 52246, USA
    Am J Physiol Heart Circ Physiol 279:H1906-12. 2000
    ..to endothelium-dependent vasodilators are absent in the aortas from mice deficient in expression of endothelial nitric oxide synthase (eNOS -/- mice), whereas responses in the cerebral microcirculation are preserved...
  52. ncbi Different vasculoprotective roles of NO synthase isoforms in vascular lesion formation in mice
    K Yogo
    Department of Cardiovascular Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Arterioscler Thromb Vasc Biol 20:E96-E100. 2000
    ..Permanent ligation of the left common carotid artery was performed in control, endothelial NOS (eNOS) knockout (eNOS-KO), and inducible NOS (iNOS) knockout (iNOS-KO) mice...
  53. pmc Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in mice
    T Matoba
    Department of Cardiovascular Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    J Clin Invest 106:1521-30. 2000
    ..In this study, we examined our hypothesis that hydrogen peroxide (H(2)O(2)) derived from endothelial NO synthase (eNOS) is an EDHF...
  54. ncbi Intercellular adhesion molecule-1 (ICAM-1) regulates endothelial cell motility through a nitric oxide-dependent pathway
    Christopher G Kevil
    Department of Pathology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    J Biol Chem 279:19230-8. 2004
    ....
  55. ncbi Dysfunction of endothelial nitric oxide synthase and atherosclerosis
    Seinosuke Kawashima
    Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho Chuo ku Kobe, 650 0017, Japan
    Arterioscler Thromb Vasc Biol 24:998-1005. 2004
    ..which represents the reduced bioavailability of nitric oxide (NO) produced from endothelial NO synthase (eNOS)...
  56. ncbi Nitric oxide synthase 1 is partly compensating for nitric oxide synthase 3 deficiency in nitric oxide synthase 3 knock-out mice and is elevated in murine and human cirrhosis
    Erwin Biecker
    Department of Clinical Pharmacology, University of Berne, Murtenstrasse, Switzerland
    Liver Int 24:345-53. 2004
    The role of endothelial nitric oxide synthase 3 (NOS-3) in the hyperdynamic circulation associated with cirrhosis is established but not that of the neuronal (NOS-1) isoform...
  57. ncbi Gab1, SHP2, and protein kinase A are crucial for the activation of the endothelial NO synthase by fluid shear stress
    Madhulika Dixit
    Vascular Signalling Group, Institut für Kardiovaskuläre Physiologie, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Circ Res 97:1236-44. 2005
    ..the activation of the phosphatidylinositol 3-kinase and the phosphorylation of the endothelial NO synthase (eNOS)...
  58. ncbi Cardiomyocyte-specific overexpression of nitric oxide synthase 3 prevents myocardial dysfunction in murine models of septic shock
    Fumito Ichinose
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA
    Circ Res 100:130-9. 2007
    ..has been implicated in the pathogenesis of septic cardiovascular dysfunction, the role of myocardial NO synthase 3 (NOS3) remains incompletely defined...
  59. ncbi Differences in inflammatory pain in nNOS-, iNOS- and eNOS-deficient mice
    Michael Karl Boettger
    Department of Neurology, Julius Maximilians University, Josef Schneider Str 11, 97080, Wurzburg, Germany
    Eur J Pain 11:810-8. 2007
    ..complete Freund's adjuvant (CFA) in wild-type (WT) mice and in mice lacking either inducible (iNOS), endothelial (eNOS) or neuronal NOS (nNOS)...
  60. ncbi Central insulin regulates heart rate and arterial blood flow: an endothelial nitric oxide synthase-dependent mechanism altered during diabetes
    Cendrine Cabou
    INSERM U858, Institute of Molecular Medicine, IFR31, CHU Rangueil, BP 84225, 31432 Toulouse Cedex 4, France
    Diabetes 56:2872-7. 2007
    ..b>Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) represents a molecular link between metabolic and cardiovascular ..
  61. doi Spontaneous myocardial infarction in mice lacking all nitric oxide synthase isoforms
    Sei Nakata
    Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Circulation 117:2211-23. 2008
    ..of the nonspecificity of the NOS inhibitors and the compensatory interactions among NOS isoforms (nNOS, iNOS, and eNOS), the ultimate roles of endogenous NO derived from the entire NOS system are still poorly understood...
  62. doi Endothelial nitric oxide deficiency reduces MMP-13-mediated cleavage of ICAM-1 in vascular endothelium: a role in atherosclerosis
    Carlos Tarin
    Fundación Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Arterioscler Thromb Vasc Biol 29:27-32. 2009
    Lack of endothelial nitric oxide synthase worsens atherosclerosis at least by increasing monocyte adhesion to endothelial cells. The purpose of this study was to elucidate the molecular mechanism elicited by NO.
  63. pmc Netrin-1 prevents ischemia/reperfusion-induced myocardial infarction via a DCC/ERK1/2/eNOS s1177/NO/DCC feed-forward mechanism
    Jun Zhang
    Division of Molecular Medicine, Cardiovascular Research Laboratories, Department of Anesthesiology, David Geffen School of Medicine at University of California Los Angeles, 650 Charles E Young Drive, Los Angeles, CA 90095, USA
    J Mol Cell Cardiol 48:1060-70. 2010
    ..In additional experiments we found netrin-1 increased ERK1/2 and eNOS(s1177) phosphorylation, and DCC protein expression, which was diminished by I/R...
  64. ncbi Hypertension in mice lacking the gene for endothelial nitric oxide synthase
    P L Huang
    Cardiovascular Research Center, Medical Services, Massachusetts General Hospital, Boston 02129, USA
    Nature 377:239-42. 1995
    ..To study the role of endothelial NOS (eNOS) in vascular function, we disrupted the gene encoding eNOS in mice...
  65. ncbi Pulmonary vasoconstriction and hypertension in mice with targeted disruption of the endothelial nitric oxide synthase (NOS 3) gene
    W Steudel
    Department of Anaesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Circ Res 81:34-41. 1997
    NO, synthesized in endothelial cells by endothelial NO synthase (NOS 3), is believed to be an important endogenous pulmonary vasodilator substance that contributes to the normal low pulmonary vascular resistance...
  66. pmc Direct evidence for the importance of endothelium-derived nitric oxide in vascular remodeling
    R D Rudic
    Department of Pharmacology, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut 06536, USA
    J Clin Invest 101:731-6. 1998
    ..In response to external carotid artery ligation, mice with targeted disruption of the endothelial nitric oxide synthase gene (eNOS) did not remodel their ipsilateral common carotid arteries whereas wild-type mice did...
  67. ncbi Coronary hemodynamics in endothelial NO synthase knockout mice
    A Godecke
    Institut für Herz und Kreislaufphysiologie, Heinrich Heine Universitat, Dusseldorf, Germany
    Circ Res 82:186-94. 1998
    For the specific analysis of endothelial NO synthase (eNOS) function in the coronary vasculature, we generated a mouse homozygous for a defective eNOS gene (eNOS-/-)...
  68. pmc Interaction of genetic deficiency of endothelial nitric oxide, gender, and pregnancy in vascular response to injury in mice
    M Moroi
    Cardiovascular Research Center and Cardiac Unit, Massachusetts General Hospital East, Charlestown, MA 02129, USA
    J Clin Invest 101:1225-32. 1998
    ..mouse model of neointimal growth; (b) evaluated the effect of disruption of a single gene, endothelial nitric oxide synthase, believed to be central to intimal growth, and (c) examined the modifying effects of gender and ..
  69. pmc Sustained pulmonary hypertension and right ventricular hypertrophy after chronic hypoxia in mice with congenital deficiency of nitric oxide synthase 3
    W Steudel
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Clin Invest 101:2468-77. 1998
    ..We investigated the effects of congenital deficiency of endothelial NO synthase (NOS3) on the pulmonary vascular responses to breathing 11% oxygen for 3-6 wk...
  70. ncbi Relative contributions of endothelial, inducible, and neuronal NOS to tone in the murine pulmonary circulation
    K A Fagan
    Cardiovascular Pulmonary Research Laboratory, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Am J Physiol 277:L472-8. 1999
    ..three isoforms of nitric oxide synthase (NOS), neuronal (nNOS, NOS I), inducible (iNOS, NOS II), and endothelial (eNOS, NOS III), are expressed in the lung...
  71. ncbi Nitric oxide synthase expression and role during cardiomyogenesis
    W Bloch
    Institute of Anatomy I, University of Cologne, Germany
    Cardiovasc Res 43:675-84. 1999
    ..The aim of the present study was the investigation of the expression of NOS during cardiomyogenesis and its functional role...
  72. ncbi Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance
    R R Shankar
    Department of Pediatrics, Indiana University School of Medicine, Indianapolis, USA
    Diabetes 49:684-7. 2000
    ..We now report on insulin sensitivity in mice with targeted disruptions in endothelial NOS (eNOS) and neuronal NOS (nNOS) genes compared with their wild-type (WT) counterparts...
  73. pmc beta(3)-adrenoceptor deficiency blocks nitric oxide-dependent inhibition of myocardial contractility
    P Varghese
    Department of Medicine, Cardiology Division, and Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    J Clin Invest 106:697-703. 2000
    ..increased in beta(3)(-/-) mice, and similar hyper-responsiveness was seen in mice lacking endothelial NO synthase (NOS3). NOS inhibition augmented isoproterenol-stimulated inotropy in wild-type (WT), but not in beta(3)(-/-) mice...
  74. ncbi Endothelial-derived nitric oxide and angiotensinogen: blood pressure and metabolism during mouse pregnancy
    L A Hefler
    Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Regul Integr Comp Physiol 280:R174-82. 2001
    ..These included mice with a null mutation in the endothelial NOS (eNOS) gene (Nos3(-/-)), four copies of the angiotensinogen gene (Agt(2/2)), and mutations in both genes [..
  75. ncbi EDHF mediates flow-induced dilation in skeletal muscle arterioles of female eNOS-KO mice
    A Huang
    Department of Physiology, New York Medical College, Valhalla, New York 10595, USA
    Am J Physiol Heart Circ Physiol 280:H2462-9. 2001
    Vasodilation to increases in flow was studied in isolated gracilis muscle arterioles of female endothelial nitric oxide synthase (eNOS)-knockout (KO) and female wild-type (WT) mice...
  76. ncbi Regulation of murine airway responsiveness by endothelial nitric oxide synthase
    M Feletou
    Institut de Recherches Servier, 92150 Suresnes, France
    Am J Physiol Lung Cell Mol Physiol 281:L258-67. 2001
    ..not the endogenous release of NO modulates bronchial tone in wild-type mice and in mice with targeted deletion of eNOS [eNOS(-/-)]...
  77. ncbi Accelerated atherosclerosis, aortic aneurysm formation, and ischemic heart disease in apolipoprotein E/endothelial nitric oxide synthase double-knockout mice
    P J Kuhlencordt
    Cardiovascular Research Center, Cardiology Division, Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA
    Circulation 104:448-54. 2001
    To test whether deficiency in endothelial nitric oxide synthase (eNOS) affects atherosclerosis development, we compared lesion formation in apolipoprotein E (apoE)/eNOS-double knockout (DKO) and apoE-knockout (KO) control animals.
  78. ncbi Hypertension does not account for the accelerated atherosclerosis and development of aneurysms in male apolipoprotein e/endothelial nitric oxide synthase double knockout mice
    J Chen
    Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Circulation 104:2391-4. 2001
    Apolipoprotein E (apoE)/endothelial nitric oxide synthase (eNOS) double knockout (DKO) mice demonstrate accelerated atherosclerosis and develop abdominal aortic aneurysms and aortic dissection, suggesting a role for eNOS in suppressing ..
  79. ncbi Neuronal NOS-dependent dilation to flow in coronary arteries of male eNOS-KO mice
    An Huang
    Department of Physiology, New York Medical College, Valhalla, New York 10595, USA
    Am J Physiol Heart Circ Physiol 282:H429-36. 2002
    ..dilation was examined in isolated coronary arteries of endothelial nitric oxide (NO) synthase knockout mice (eNOS-KO) and wild-type (WT) mice...
  80. ncbi Decreased arteriolar density in endothelial nitric oxide synthase knockout mice is due to hypertension, not to the constitutive defect in endothelial nitric oxide synthase enzyme
    Nathalie Kubis
    INSERM Unit 541, Hopital Lariboisiere, Paris, France
    J Hypertens 20:273-80. 2002
    Hypertension in endothelial nitric oxide synthase knockout (eNOS-/-) mice is believed to be partly due to altered vasodilatation. However, nitric oxide (NO) is also known to play an important part in angiogenesis.
  81. pmc Oxidation of the zinc-thiolate complex and uncoupling of endothelial nitric oxide synthase by peroxynitrite
    Ming Hui Zou
    Vascular Biology Unit, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    J Clin Invest 109:817-26. 2002
    ..present here the first evidence that peroxynitrite (ONOO(-)) releases zinc from the zinc-thiolate cluster of endothelial NOS (eNOS) and presumably forms disulfide bonds between the monomers...
  82. ncbi Differential response to myocardial reperfusion injury in eNOS-deficient mice
    Brent R Sharp
    Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Am J Physiol Heart Circ Physiol 282:H2422-6. 2002
    Two strains of endothelial nitric oxide synthase (eNOS)-deficient (-/-) mice have been developed that respond differently to myocardial ischemia-reperfusion (MI/R). We evaluated both strains of eNOS(-/-) mice in an in vivo model of MI/R...
  83. ncbi Role of microvascular rarefaction in the increased arterial pressure in mice lacking for the endothelial nitric oxide synthase gene (eNOS3pt-/- )
    Nathalie Kubis
    Unité INSERM 541 Hôpital Lariboisière, Paris, France
    J Hypertens 20:1581-7. 2002
    Mechanisms involved in hypertension in homozygous mice for the defective endothelial nitric oxide synthase gene (eNOS-/-) have not been fully elucidated...
  84. ncbi Development of heart failure and congenital septal defects in mice lacking endothelial nitric oxide synthase
    Qingping Feng
    Department of Medicine, London Health Sciences Centre, London, Ontario, Canada
    Circulation 106:873-9. 2002
    Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays an important role in the regulation of cell growth, apoptosis, and tissue perfusion...
  85. ncbi Cardiovascular and renal control in NOS-deficient mouse models
    Pablo A Ortiz
    Division of Hypertension and Vascular Research, Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan 48202, USA
    Am J Physiol Regul Integr Comp Physiol 284:R628-38. 2003
    ..Endogenous NO is produced by three different NO synthase (NOS) isoforms: endothelial NOS (eNOS), inducible NOS (iNOS), and neuronal NOS (nNOS)...
  86. ncbi Mitochondrial biogenesis in mammals: the role of endogenous nitric oxide
    Enzo Nisoli
    Department of Preclinical Sciences, Center for Study and Research on Obesity, Luigi Sacco Hospital, University of Milan, Milan 20157, Italy
    Science 299:896-9. 2003
    ..mitochondrial biogenesis induced by exposure to cold was markedly reduced in brown adipose tissue of endothelial nitric oxide synthase null-mutant (eNOS-/-) mice, which had a reduced metabolic rate and accelerated weight gain as ..
  87. ncbi Macrophage endothelial nitric-oxide synthase autoregulates cellular activation and pro-inflammatory protein expression
    Linda Connelly
    Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 278:26480-7. 2003
    ..However, macrophages have also been shown to express a constitutive NOS isoform, the function of which remains obscure...
  88. ncbi Chaperone-dependent regulation of endothelial nitric-oxide synthase intracellular trafficking by the co-chaperone/ubiquitin ligase CHIP
    Jihong Jiang
    Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, North Carolina 27599 7075, USA
    J Biol Chem 278:49332-41. 2003
    Endothelial nitric-oxide synthase (eNOS), the enzyme responsible for production of endothelial NO, is under tight and complex regulation...
  89. ncbi Defective lung vascular development and fatal respiratory distress in endothelial NO synthase-deficient mice: a model of alveolar capillary dysplasia?
    Robin N N Han
    Terrence Donnelly Heart Center, Division of Cardiology, St Michael s Hospital and the Department of Medicine, University of Toronto, Toronto, Canada
    Circ Res 94:1115-23. 2004
    ..Although endothelial NO synthase (eNOS)-deficient mice are viable, minor congenital cardiac abnormalities have been reported and homozygous offspring ..
  90. ncbi Role of endothelial nitric oxide synthase in endothelial activation: insights from eNOS knockout endothelial cells
    Peter J Kuhlencordt
    Cardiovascular Research Center, Massachusetts General Hospital East, 149 East 13th St, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 286:C1195-202. 2004
    The objective of this study was to determine whether absence of endothelial nitric oxide synthase (eNOS) affects the expression of cell surface adhesion molecules in endothelial cells...
  91. ncbi Nitric oxide, a key signaling molecule in the murine early embryonic heart
    Daniela Malan
    Department of Animal and Human Biology, INFM University of Turin, Italy
    FASEB J 18:1108-10. 2004
    ..NOS) isoform(s), we analyzed muscarinic signaling in embryonic ventricular cardiomyocytes of NOS2 (-/-) and NOS3 (-/-) mice...
  92. ncbi Partial gene deletion of endothelial nitric oxide synthase predisposes to exaggerated high-fat diet-induced insulin resistance and arterial hypertension
    Stephane Cook
    Department of Internal Medicine and the Botnar Center for Clinical Research, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Diabetes 53:2067-72. 2004
    ..and metabolic homeostasis, as evidenced by insulin resistance and arterial hypertension in endothelial NO synthase (eNOS) null mice...
  93. ncbi Differential roles of neuronal and endothelial nitric oxide synthases during carrageenan-induced inflammatory hyperalgesia
    F Tao
    Department of Anesthesiology, Johns Hopkins University School of Medicine, 600 North Wolfe Street Blalock 1415, Baltimore, MD 21287, USA
    Neuroscience 128:421-30. 2004
    ..Furthermore, intrathecal administration of the endothelial NOS (eNOS) inhibitor L-N5-(1-iminoethyl)-ornithinein nNOS knockout mice inhibited the thermal hyperalgesia in ..
  94. pmc Endothelial nitric oxide synthase activation is critical for vascular leakage during acute inflammation in vivo
    Mariarosaria Bucci
    Dipartimento di Farmacologia Sperimentale, Via Domenico Montesano 49, Universita di Napoli Federico II, 80131 Naples, Italy
    Proc Natl Acad Sci U S A 102:904-8. 2005
    ..Here, we examine acute inflammation in congenic endothelial NO synthase-deficient (eNOS-/-) mice. Intraplantar injection of carrageenan induces a biphasic inflammatory response...
  95. ncbi Resistance to endotoxic shock in endothelial nitric-oxide synthase (eNOS) knock-out mice: a pro-inflammatory role for eNOS-derived no in vivo
    Linda Connelly
    Wolfson Institute for Biomedical Research, University College London, Cruciform Building, Gower Street, London WC1E 6AE, UK
    J Biol Chem 280:10040-6. 2005
    ..We have shown previously that NO from the constitutive endothelial NOS (eNOS) is necessary to obtain maximal iNOS expression and activity following exposure of murine macrophages ..
  96. ncbi Local atrial natriuretic peptide signaling prevents hypertensive cardiac hypertrophy in endothelial nitric-oxide synthase-deficient mice
    Alexander Bubikat
    Department of Pharmacology and Toxicology, Universitatsklinikum Munster, D 48129 Munster, Germany
    J Biol Chem 280:21594-9. 2005
    ..inactivation of either the guanylyl cyclase-A receptor for ANP (GC-A-/-) or endothelial nitric-oxide synthase (eNOS-/-)...
  97. pmc Endothelial nitric oxide synthase is critical for ischemic remodeling, mural cell recruitment, and blood flow reserve
    Jun Yu
    Department of Pharmacology and Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536, USA
    Proc Natl Acad Sci U S A 102:10999-1004. 2005
    The genetic loss of endothelial-derived nitric oxide synthase (eNOS) in mice impairs vascular endothelial growth factor (VEGF) and ischemia-initiated blood flow recovery resulting in critical limb ischemia...
  98. pmc Atheroprotective effects of neuronal nitric oxide synthase in apolipoprotein e knockout mice
    Peter J Kuhlencordt
    Medizinische Klinik, University Wuerzburg, Germany
    Arterioscler Thromb Vasc Biol 26:1539-44. 2006
    ..To test whether neuronal NOS (nNOS) deficiency affects atherosclerosis, we studied apoE/nNOSalpha double knockout (DKO) and apolipoprotein E (apoE) knockout (KO) control mice...
  99. ncbi Cardiovascular function in mice during normal pregnancy and in the absence of endothelial NO synthase
    Shathiyah Kulandavelu
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Hypertension 47:1175-82. 2006
    ..C57Bl/6J (B6) mice show similar changes and whether these changes are altered in mice lacking the gene for endothelial NOS (eNOS). The CO of B6 mice increased 28% by day 9...
  100. ncbi eNOS gene therapy exacerbates hepatic ischemia-reperfusion injury in diabetes: a role for eNOS uncoupling
    John W Elrod
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Circ Res 99:78-85. 2006
    Previous studies indicate that endothelial nitric oxide synthase (eNOS) function is impaired in diabetes as a result of increased vascular generation of reactive oxygen species. We hypothesized that eNOS gene therapy would augment NO...
  101. ncbi Genetic overexpression of eNOS attenuates hepatic ischemia-reperfusion injury
    Mark R Duranski
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Am J Physiol Heart Circ Physiol 291:H2980-6. 2006
    Previous studies have shown that endothelial nitric oxide (NO) synthase (eNOS)-derived NO is an important signaling molecule in ischemia-reperfusion (I-R) injury...

Research Grants39

  1. Genetics of Pulmonary Hypertension in Mice
    Karen Fagan; Fiscal Year: 2006
    ..abstract_text> ..
  2. Effects of BMPRII Mutations in Pulmonary Hypertension
    Karen Fagan; Fiscal Year: 2006
    ..We will have also tested the role of four pulmonary cell types, smooth muscle, endothelium, airway epithelium and macrophages in the link between BMPR2 and pulmonary hypertension. ..
  3. 2006 Grover Conference on the Pulmonary Circulation - Rho Family GTPases
    Karen Fagan; Fiscal Year: 2006
    ..The ranch is an intimate setting that encourages interaction between scientists and clinicians. We are requesting funding to support travel and lodging for invited speakers. [unreadable] [unreadable] [unreadable] [unreadable]..
  4. Posttranslational Regulation of eNOS by Estrogen in the Vagina and Clitoris
    Biljana Musicki; Fiscal Year: 2007
    ..Vascular effects of estrogen are attributed to its regulation of endothelial nitric oxide synthase (eNOS), yet the physiological role of nitric oxide (NO) and the molecular mechanisms of eNOS ..
  5. VEGF and Nitric Oxide in Lung Vascular Development
    Vivek Balasubramaniam; Fiscal Year: 2007
    ..In this proposal, he will use a transgeneic mouse model that is deficient in endothelial nitric oxide synthase that develops pulmonary hypoplasia with exposure to mild neonatal hypoxia...
  6. NITRIC OXIDE REGULATORY SYSTEM IN THE PENIS
    Arthur Burnett; Fiscal Year: 2002
    ....
  7. 2008 AUA/SBUR Summer Research Conference
    Arthur Burnett; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  8. In Utero Programming of Adult Vascular Function
    Monica Longo; Fiscal Year: 2010
    ..As endothelial nitric oxide synthase (NOS3) is important in determining vascular function in adults, and deficiency in its function leads ..
  9. Salt Absorption by the THAL: Role of NKCC2 trafficking
    Pablo A Ortiz; Fiscal Year: 2010
    ..Data from this proposal will increase our understanding of how salt absorption by the THAL is regulated in normal and salt-sensitive animals, and will identify new targets for the development of diuretics. ..
  10. eNOS-Protein Interaction in the Penis
    Biljana Musicki; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Endothelial nitric oxide synthase (eNOS) is an important mediator of penile erection...
  11. PO2 MODULATION OF NO AND ET1 IN PULMONARY HYPERTENSION
    Karen Fagan; Fiscal Year: 2002
    ..Our laboratory is interested in the role of endogenously derived nitric oxide (NO) from eNOS (endothelial derived nitric oxide synthase), endothelin-1 (ET-1), and the effect of hypoxia on the development of ..
  12. Role of KLF2 in erythropoiesis and globin expression
    Joyce A Lloyd; Fiscal Year: 2010
    ..Our long-term goal is to use KLF2 in strategies to increase embryonic/fetal beta-globin, to treat patients with beta-hemoglobinopathies. ..
  13. Caveolin-1 and NO Regulate PMN-mediated Increases in Vascular Permeability
    Richard D Minshall; Fiscal Year: 2011
    ..in endothelial permeability and lung injury, and (ii) PMN activation of Src kinase in endothelial cells regulates eNOS-mediated NO production via PI3-kinase and Akt signaling and that the NO derived from PMN-endothelial interaction ..
  14. Ethanol Prevents Microvascular Dysfunction
    Ronald J Korthuis; Fiscal Year: 2010
    ..Our overall hypothesis is that ethanol-induced, AMPK-dependent eNOS activation serves as an important initiating factor to trigger a cascade of signaling events that ultimately act to ..
  15. Venular leukocyte adhesion, impaired arteriolar vasoreactivity, and intestinal IR
    Ronald J Korthuis; Fiscal Year: 2010
    ..Given the importance of the endothelium in regulating vascular tone, these fundamentally important findings have enormous implications for our understanding of blood flow dysregulation in conditions characterized by I/R. ..
  16. EXTRACELLULAR SUPEROXIDE DISMUTASE AND ATHEROSCLEROSIS
    David Harrison; Fiscal Year: 2001
    ....
  17. Endothelial Function in the Cerebral Circulation
    Frank Faraci; Fiscal Year: 2009
    ..These studies should provide new insight into mechanisms of cerebral vascular protection during hypertension. ..
  18. Gender Influence in Mice with Myocardial Infarction
    Xiao Ping Yang; Fiscal Year: 2009
    ....
  19. Nitric Oxide/cGMP/Endotoxin-Induced Myocardial Dysfunct
    Fumito Ichinose; Fiscal Year: 2008
    ....
  20. 2007 Vascular Cell Biology Gordon Research Conference
    Bradford Berk; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. FLOW SHEAR INDUCED NF-KB MEDIATED REGULATION
    Sumathy Mohan; Fiscal Year: 2003
    ..Understanding of NF- kappaB signaling mechanisms in VCAM-1 regulation can also be applied to other inflammatory conditions. ..
  22. PRECONDITIONING: PMN ADHESION AND MICROVASCULAR INJURY
    RONALD KORTHUIS; Fiscal Year: 2003
    ..To address this issue, we propose to determine whether: (1) NO derived from endothelial NOS is produced during the period of preconditioning ischemia and triggers the protective actions of delayed IPC; ..
  23. Zeiss Dynamic Laser TIRF
    Richard Minshall; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  24. Study of Intrinsic Bladder Activity by Optical Imaging
    Anthony Kanai; Fiscal Year: 2007
    ..To study the neural-chemical control of spontaneous detrusor activity; and III.) To determine the origin of detrusor overactivity in partially obstructed bladders. ..
  25. REGULATION OF HUMAN GAMMA AND BETA GLOBIN GENES
    Joyce Lloyd; Fiscal Year: 2003
    ..These results will predict the proteins involved in early beta-globin gene suppression. ..
  26. RENAL MEDULLARY NITRIC OXIDE AND BLOOD PRESSURE
    David Mattson; Fiscal Year: 2001
    ....
  27. Pathogenic mechanisms that initiate DMD
    Robert Grange; Fiscal Year: 2006
    ..These studies will provide valuable insight for the development of therapeutic interventions to prevent the onset of DMD. ..
  28. MORPHOLOGY AND FUNCTION OF BRAIN VESSELS
    GARY BAUMBACH; Fiscal Year: 2006
    ..These studies should provide important new insight into the mechanisms of vascular remodeling. [unreadable] [unreadable] [unreadable]..
  29. Endothelial Deformation and Coronary Arteriolar Function
    Dong Sun; Fiscal Year: 2006
    ....
  30. HYPERGLYCEMIA INHIBITION OF GLOMERULAR eNOS
    Shaoyou Chu; Fiscal Year: 2004
    ..This proposal will test a hypothesis that eNOS (endothelial nitric oxide synthase) of glomerular endothelia is a direct target of hyperglycemia...
  31. CARDIOVASCULAR RESEARCH TRAINING
    Bradford Berk; Fiscal Year: 2004
    ..The University of Rochester is a research-intensive institution with a long tradition and commitment to training investigators which should facilitate the success of this program in cardiovascular research. ..
  32. Angiogenic Agents and Alveolar Injury
    Vineet Bhandari; Fiscal Year: 2007
    ..Determine the differences and the degree to which the response to hyperoxia-induced acute lung injury differs in adult, newborn and fetal mice with regard to VEGF, Angiopoietin 1 and Angiopoietin 2. ..
  33. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  34. Effects of Nitric Oxide on Stem Cell Differentiation
    Zhenguo Liu; Fiscal Year: 2008
    In a pilot study we showed that endothelial nitric oxide synthase (eNOS) was dynamically expressed during the course of stem cell differentiation into endothelial cells in vitro...
  35. Nitric Oxide Regulation of Compensatory Lung Growth
    VICTOR LAUBACH; Fiscal Year: 2005
    ..To test this hypothesis, knockout mice deficient in endothelial NO synthase (eNOS), inducible NOS (iNOS), and both eNOS/iNOS will be used to study NO-mediated mechanisms of post-pneumonectomy CLG...
  36. MOLECULAR CONTROL OF CELL MIGRATION IN THE SPINAL CORD
    JOSEPH YIP; Fiscal Year: 2004
    ..Results will provide important insights on the molecular and cellular basis of Reelin function, and may reveal significant information about the mechanisms responsible for devastating diseases caused by abnormal migration. ..
  37. GENETICS OF NEUROPEPTIDE Y FUNCTION
    RICHARD PALMITER; Fiscal Year: 2001
    ..These genetic experiments should help rationalize the current disparate results obtained by permanent NPY gene silencing and by acute intervention of NPY signaling. ..