Nanog

Summary

Gene Symbol: Nanog
Description: Nanog homeobox
Alias: 2410002E02Rik, ENK, ecat4, homeobox protein NANOG, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox transcription factor Nanog
Species: mouse

Top Publications

  1. pmc Global mapping of DNA methylation in mouse promoters reveals epigenetic reprogramming of pluripotency genes
    Cassandra R Farthing
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, United Kingdom
    PLoS Genet 4:e1000116. 2008
  2. pmc Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions
    Thorold W Theunissen
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Curr Biol 21:65-71. 2011
  3. ncbi Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
  4. pmc Nanog is the gateway to the pluripotent ground state
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK
    Cell 138:722-37. 2009
  5. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
  6. ncbi The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Nat Genet 38:431-40. 2006
  7. ncbi Nanog expression in mouse germ cell development
    Shinpei Yamaguchi
    Laboratory of Stem Cell Engineering, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo ku, Japan
    Gene Expr Patterns 5:639-46. 2005
  8. pmc Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal
    Kathy K Niakan
    Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 24:312-26. 2010
  9. ncbi Dissecting self-renewal in stem cells with RNA interference
    Natalia Ivanova
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nature 442:533-8. 2006
  10. ncbi Pluripotential competence of cells associated with Nanog activity
    Shin Ya Hatano
    Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Mech Dev 122:67-79. 2005

Scientific Experts

Detail Information

Publications132 found, 100 shown here

  1. pmc Global mapping of DNA methylation in mouse promoters reveals epigenetic reprogramming of pluripotency genes
    Cassandra R Farthing
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, United Kingdom
    PLoS Genet 4:e1000116. 2008
    ..Comparisons between pluripotent tissues and pMEFs reveal that a number of pluripotency related genes, including Nanog, Lefty1 and Tdgf1, as well as the nucleosome remodeller Smarcd1, are hypomethylated in stem cells and ..
  2. pmc Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions
    Thorold W Theunissen
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Curr Biol 21:65-71. 2011
    ..Here we show that 2i/LIF treatment in clonal lines of pre-iPS cells results in the activation of endogenous Nanog and rapid downregulation of retroviral Oct4 expression...
  3. ncbi Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
    b>Nanog is a divergent homeodomain protein found in mammalian pluripotent cells and developing germ cells...
  4. pmc Nanog is the gateway to the pluripotent ground state
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK
    Cell 138:722-37. 2009
    ..Here we present evidence that the homeodomain protein Nanog mediates acquisition of both embryonic and induced pluripotency...
  5. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
    ..We found that one of them, encoding the homeoprotein Nanog, was capable of maintaining ES cell self-renewal independently of LIF/Stat3...
  6. ncbi The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Nat Genet 38:431-40. 2006
    Oct4 and Nanog are transcription factors required to maintain the pluripotency and self-renewal of embryonic stem (ES) cells...
  7. ncbi Nanog expression in mouse germ cell development
    Shinpei Yamaguchi
    Laboratory of Stem Cell Engineering, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo ku, Japan
    Gene Expr Patterns 5:639-46. 2005
    b>Nanog is a newly identified transcriptional factor bearing a homeodomain and expressed in pluripotential cells of preimplantation and early postimplantation embryos, and embryonic stem (ES) and embryonic germ (EG) cells...
  8. pmc Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal
    Kathy K Niakan
    Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 24:312-26. 2010
    ..cell types and maintain expression of pluripotency-associated transcription factors, including Oct4, Nanog, and Sox2...
  9. ncbi Dissecting self-renewal in stem cells with RNA interference
    Natalia Ivanova
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nature 442:533-8. 2006
    ..Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells...
  10. ncbi Pluripotential competence of cells associated with Nanog activity
    Shin Ya Hatano
    Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Mech Dev 122:67-79. 2005
    b>Nanog is a novel pluripotential cell-specific gene that plays a crucial role in maintaining the undifferentiated state of early postimplantation embryos and embryonic stem (ES) cells...
  11. ncbi Transcriptional regulation of nanog by OCT4 and SOX2
    David J Rodda
    Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    J Biol Chem 280:24731-7. 2005
    b>Nanog, Sox2, and Oct4 are transcription factors all essential to maintaining the pluripotent embryonic stem cell phenotype...
  12. ncbi Regulation of the pluripotency marker Rex-1 by Nanog and Sox2
    Wenjing Shi
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 281:23319-25. 2006
    ..However, the mechanism by which Rex-1 is regulated in pluripotent cells remains unresolved. Here we report that Nanog, an Nk-2 homeodomain protein known for its role in maintaining stem cell pluripotency, is a transcription ..
  13. pmc The Grb2/Mek pathway represses Nanog in murine embryonic stem cells
    Takashi Hamazaki
    Department of Pathology, University of Florida College of Medicine, P O Box 100275, Gainesville, FL 32610, USA
    Mol Cell Biol 26:7539-49. 2006
    The homeobox gene Nanog is a key intrinsic determinant of self renewal in embryonic stem (ES) cells, and its repression leads ES cells to selectively differentiate into primitive endoderm...
  14. pmc Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst
    Berenika Plusa
    Developmental Biology Program, Sloan Kettering Institute, New York, USA
    Development 135:3081-91. 2008
    ..Subsequently, a gradual progression towards a mutually exclusive expression of PrE- and EPI-specific markers occurs. Finally, cell sorting is achieved by a variety of cell behaviours and by selective apoptosis...
  15. pmc Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression
    Peilin Zhang
    Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 285:9180-9. 2010
    ..In addition, Klf4 directly binds to the promoter region of Nanog and regulates its expression...
  16. ncbi Expression of Nanog gene promotes NIH3T3 cell proliferation
    Jingyu Zhang
    Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
    Biochem Biophys Res Commun 338:1098-102. 2005
    ..However, there are numbers of limitations for in vitro cell proliferation. Nanog is an important self-renewal determinant in embryonic stem cells...
  17. ncbi Beta-catenin signaling contributes to stemness and regulates early differentiation in murine embryonic stem cells
    Roman Anton
    Institute for Biochemistry and Molecular Biology, Ulm University, D 89069 Ulm, Germany
    FEBS Lett 581:5247-54. 2007
    ..Oct-3/4, nanog and Wnt11 were able to repress TCF/beta-catenin transcriptional activity...
  18. doi The pluripotency rheostat Nanog functions as a dimer
    Nicholas P Mullin
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, U K
    Biochem J 411:227-31. 2008
    The defining activity of the homeodomain protein Nanog is the ability to confer cytokine-independent self-renewal upon ES (embryonic stem) cells in which it is overexpressed...
  19. pmc Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog
    Keigo Machida
    Southern California Research Center for Alcoholic, Liver, and Pancreatic Diseases and Cirrhosis, and Department of Molecular Microbiology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Proc Natl Acad Sci U S A 106:1548-53. 2009
    ..We also identify Nanog, the stem/progenitor cell marker, as a novel downstream gene up-regulated by TLR4 activation and the presence of ..
  20. doi The pluripotency regulator Zic3 is a direct activator of the Nanog promoter in ESCs
    Linda Shushan Lim
    Stem Cell and Developmental Biology Group, Genome Institute of Singapore, Singapore
    Stem Cells 28:1961-9. 2010
    ..ChIP results and in vitro DNA binding assays revealed that Zic3 binds with high affinity and specificity on the Nanog promoter...
  21. pmc NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis
    Erin E Kohler
    Department of Pharmacology, University of Illinois, Chicago, IL 60612, USA
    Blood 117:1761-9. 2011
    b>NANOG is a master transcription factor associated with the maintenance of stem cell pluripotency. Here, we demonstrate that transcription factor NANOG is expressed in cultured endothelial cells (ECs) and in a subset of tumor cell lines...
  22. ncbi Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst
    Dan Strumpf
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Ontario, Canada
    Development 132:2093-102. 2005
    ..to ICM lineage is attributed to the function of the two transcription factors, Oct4 (encoded by Pou5f1) and Nanog. However, a positive regulator of TE cell fate has not been described...
  23. ncbi Nanog and transcriptional networks in embryonic stem cell pluripotency
    Guangjin Pan
    The Wisconsin National Primate Research Center, University of Wisconsin Madison, 1220 Capitol Court, Madison, WI 53715 1299, USA
    Cell Res 17:42-9. 2007
    ..A unique homeobox transcription factor, Nanog, is one of the key downstream effectors of these signals...
  24. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..We applied expression cloning in mouse ES cells to isolate a self-renewal determinant. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells...
  25. ncbi Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human
    Adam H Hart
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    Dev Dyn 230:187-98. 2004
    The murine Nanog gene, a member of the homeobox family of DNA binding transcription factors, has been shown recently to maintain pluripotency of embryonic stem cells...
  26. ncbi The stem cell pluripotency factor NANOG activates transcription with two unusually potent subdomains at its C terminus
    Guangjin Pan
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, Institutes of Biomedicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, 100084 Beijing, China
    J Biol Chem 280:1401-7. 2005
    ..b>NANOG, an NK-2 type homeodomain gene, has been proposed to play a key role in maintaining stem cell pluripotency ..
  27. ncbi p53 induces differentiation of mouse embryonic stem cells by suppressing Nanog expression
    Tongxiang Lin
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0322, USA
    Nat Cell Biol 7:165-71. 2005
    ..p53 binds to the promoter of Nanog, a gene required for ESC self-renewal, and suppresses Nanog expression after DNA damage...
  28. ncbi Nanog retrotransposed genes with functionally conserved open reading frames
    Morag Robertson
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JQ, Scotland
    Mamm Genome 17:732-43. 2006
    The Nanog gene plays a key role in the pluripotency of early embryonic cells in vitro and in vivo. In this article retrotransposed copies of Nanog, termed NanogPc and NanogPd, are identified on mouse Chromosomes 4 and 7, respectively...
  29. ncbi A heterogeneous expression pattern for Nanog in embryonic stem cells
    Amar M Singh
    Department of Pathology, University of Florida College of Medicine, Box 100275, Gainesville, Florida 32610, USA
    Stem Cells 25:2534-42. 2007
    b>Nanog is a critical homeodomain factor responsible for maintaining embryonic stem (ES) cell self-renewal and pluripotency. Of interest, Nanog expression is not homogeneous in the conventional culture of murine ES cells...
  30. ncbi Sox17 plays a substantial role in late-stage differentiation of the extraembryonic endoderm in vitro
    Masafumi Shimoda
    Department of Surgery, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Cell Sci 120:3859-69. 2007
    ..This event was inhibited by the forced expression of Nanog, a negative regulator of differentiation of ESCs into the ExE...
  31. ncbi Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos
    Noriyuki Nishioka
    Laboratory for Embryonic Induction, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe, Hyogo 650 0047, Japan
    Mech Dev 125:270-83. 2008
    ..Instead, the ICM specific transcription factors, Oct3/4 and Nanog, were expressed in all the blastomeres...
  32. doi A core Klf circuitry regulates self-renewal of embryonic stem cells
    Jianming Jiang
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, 02 01, Genome Building, Singapore 138672
    Nat Cell Biol 10:353-60. 2008
    ..immunoprecipitation coupled to microarray assay reveals that these Klf proteins share many common targets of Nanog, suggesting a close functional relationship between these factors...
  33. doi MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation
    Yvonne Tay
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Agency for Science Technology and Research, 08 01, Genome, 60 Biopolis Street, Singapore 138672, Singapore
    Nature 455:1124-8. 2008
    ..Here we focus on the mouse Nanog, Oct4 (also known as Pou5f1) and Sox2 genes and demonstrate the existence of many naturally occurring miRNA ..
  34. pmc Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells
    Tibor Kalmar
    Department of Genetics, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 7:e1000149. 2009
    ..stem (ES) cells is associated with the activity of a network of transcription factors with Sox2, Oct4, and Nanog at the core...
  35. pmc Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 136:3215-22. 2009
    ..Throughout the ICM, the epiblast-specific marker Nanog is expressed, and in XX embryos epigenetic silencing of the paternal X chromosome is erased...
  36. doi Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells
    Shinpei Yamaguchi
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Development 136:4011-20. 2009
    The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation...
  37. pmc Global chromatin architecture reflects pluripotency and lineage commitment in the early mouse embryo
    Kashif Ahmed
    Genetics and Genome Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada
    PLoS ONE 5:e10531. 2010
    ..Together, these studies show that an open chromatin architecture is established in the embryonic lineages during development and is sufficient to distinguish pluripotent cells from tissue-restricted progenitor cells...
  38. pmc LRH-1 and Nanog regulate Dax1 transcription in mouse embryonic stem cells
    Victoria R Kelly
    Program in Cellular and Molecular Biology, United States
    Mol Cell Endocrinol 332:116-24. 2011
    ..Predicated on recent work indicating that Nanog binds to the Dax1 intron, we have used chromatin immunoprecipitation experiments (ChIP) to define an intronic site ..
  39. doi Control of ground-state pluripotency by allelic regulation of Nanog
    Yusuke Miyanari
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM U964, Universite de Strasbourg, F 67404 Illkirch, Cité Universitaire de Strasbourg, France
    Nature 483:470-3. 2012
    ..involves both the resetting of epigenetic marks and the activation of pluripotent-cell-specific genes such as Nanog and Oct4 (also known as Pou5f1)...
  40. doi Correlations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cells
    Silvia Muñoz Descalzo
    Department of Genetics, University of Cambridge, Cambridge, United Kingdom
    Stem Cells 30:2683-91. 2012
    ..pluripotent state is traditionally associated with large absolute levels of certain transcription factors such as Nanog and Oct4...
  41. pmc NANOG-dependent function of TET1 and TET2 in establishment of pluripotency
    Yael Costa
    Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nature 495:370-4. 2013
    ..thought to reside in a core circuitry of master transcription factors including the homeodomain-containing protein NANOG, which has an essential role in establishing ground state pluripotency during somatic cell reprogramming...
  42. pmc A genetic and developmental pathway from STAT3 to the OCT4-NANOG circuit is essential for maintenance of ICM lineages in vivo
    Dang Vinh Do
    Cancer Science Institute of Singapore, Singapore 117599, Singapore
    Genes Dev 27:1378-90. 2013
    Although it is known that OCT4-NANOG are required for maintenance of pluripotent cells in vitro, the upstream signals that regulate this circuit during early development in vivo have not been identified...
  43. doi FGF4 is a limiting factor controlling the proportions of primitive endoderm and epiblast in the ICM of the mouse blastocyst
    Dayana Krawchuk
    Goodman Cancer Research Centre, Department of Human Genetics, McGill University, 1160 Pine Avenue West, Rm 419, Canada
    Dev Biol 384:65-71. 2013
    ..We conclude that the amount of FGF4 is limited and regulates PE/EPI proportions in the mouse embryo...
  44. pmc Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst
    Gloryn Chia Le Bin
    Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 141:1001-10. 2014
    ..maternal and zygotic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog expression...
  45. pmc GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst
    Nadine Schrode
    Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Dev Cell 29:454-67. 2014
    ..differentiate into the pluripotent epiblast or the primitive endoderm (PrE), marked by the transcription factors NANOG and GATA6, respectively...
  46. doi Stochastic NANOG fluctuations allow mouse embryonic stem cells to explore pluripotency
    Elsa Abranches
    Instituto de Medicina Molecular and Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa, Avenida Prof Egas Moniz, Lisboa 1649 028, Portugal Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Avenida Brasilia Doca de Pedrouços, Lisboa 1400 038, Portugal
    Development 141:2770-9. 2014
    Heterogeneous expression of the transcription factor NANOG has been linked to the existence of various functional states in pluripotent stem cells...
  47. ncbi Eleven daughters of NANOG
    H Anne F Booth
    Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK
    Genomics 84:229-38. 2004
    b>Nanog is a recently discovered ANTP class homeobox gene. Mouse Nanog is expressed in the inner cell mass and in embryonic stem cells and has roles in self-renewal and maintenance of pluripotency...
  48. ncbi Isolation and characterization of the murine Nanog gene promoter
    Da Yong Wu
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Cell Res 15:317-24. 2005
    b>Nanog protein is expressed in the interior cells of compacted morulae and maintained till epiblasts but downregulated by implantation stage...
  49. ncbi Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog
    Shelley R Hough
    Research and Development Division, Gene Regulation Group, Invitrogen Corporation, Carlsbad, California 92008, USA
    Stem Cells 24:1467-75. 2006
    ..After transfection of embryonic stem cells with OCT4- or Nanog-targeted RNAi compounds, levels of OCT4 or Nanog transcript and protein were reduced accordingly...
  50. ncbi Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
    ..5 is already heterogeneous. The EPI- and PE-specific transcription factors, Nanog and Gata6, were expressed in the ICM in a random "salt and pepper" pattern, as early as E3...
  51. ncbi Nanog promotes transfer of pluripotency after cell fusion
    Jose Silva
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, EH9 3JQ, UK
    Nature 441:997-1001. 2006
    ..One candidate is the variant homeodomain protein Nanog, which has the capacity to entrain undifferentiated ES cell propagation...
  52. pmc Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells
    Atsushi Suzuki
    Gene Expression Laboratory, Stem Cell Research Center, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 103:10294-9. 2006
    ..BMP) and leukemia inhibitory factor signaling pathways and the expression of the transcription factors Oct4 and Nanog. However, the network of interactions among extrinsic and intrinsic determinants of ES cell pluripotency is ..
  53. ncbi Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells
    Qiang Wu
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore
    J Biol Chem 281:24090-4. 2006
    Embryonic stem (ES) cells are pluripotent cells with self-renewing property. Nanog is a homeobox transcription factor required to maintain ES cells in a non-differentiated state...
  54. pmc Repression of Nanog gene transcription by Tcf3 limits embryonic stem cell self-renewal
    Laura Pereira
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 900 S Ashland Ave, Molecular Biology Research Building, Room 1268, Mail Code 669, Chicago, IL 60607, USA
    Mol Cell Biol 26:7479-91. 2006
    ..and differentiation, ESC must carefully control the levels of several transcription factors, including Nanog, Sox2, and Oct4...
  55. pmc Murine inner cell mass-derived lineages depend on Sall4 function
    Ulrich Elling
    Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:16319-24. 2006
    ..These data establish Sall4 as an essential transcription factor required for the early development of inner cell mass-derived cell lineages...
  56. ncbi Regulation of Nanog expression by phosphoinositide 3-kinase-dependent signaling in murine embryonic stem cells
    Mike P Storm
    Department of Pharmacy and Pharmacology and Centre for Regenerative Medicine, The University of Bath, Bath BA2 7AY, United Kingdom
    J Biol Chem 282:6265-73. 2007
    ..pharmacological or genetic tools results in decreased expression of RNA for the homeodomain transcription factor Nanog and decreased Nanog protein levels...
  57. ncbi Epigenetic regulation of Nanog gene in embryonic stem and trophoblast stem cells
    Naoko Hattori
    Laboratory of Cellular Biochemistry, Department of Animal Resource Sciences Veterinary Medical Sciences, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Genes Cells 12:387-96. 2007
    The Nanog and Oct-4 genes are essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos...
  58. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells...
  59. doi Nanog maintains pluripotency of mouse embryonic stem cells by inhibiting NFkappaB and cooperating with Stat3
    Josema Torres
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 10:194-201. 2008
    ..The transcription factor Nanog is essential in maintaining pluripotency but the mechanisms involved are poorly understood...
  60. doi Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution
    Nimet Maherali
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, MA 02114, USA
    Cell Stem Cell 1:55-70. 2007
    ....
  61. pmc Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells
    Tobias Brambrink
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell Stem Cell 2:151-9. 2008
    ..Expression of Nanog and the endogenous Oct4 gene, marking fully reprogrammed cells, was only observed late in the process...
  62. pmc ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a
    Xiaolin Gao
    Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Richard Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 105:6656-61. 2008
    ..Our results suggest that BAF250a is a key component of the gene regulatory machinery in ES cells controlling self-renewal, differentiation, and cell lineage decisions...
  63. doi Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells
    Jiancong Liang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Cell Biol 10:731-9. 2008
    b>Nanog and Oct4 are essential transcription factors that regulate self-renewal and pluripotency of ES cells. However, the mechanisms by which Nanog and Oct4 modulate ES cell fate remain unknown...
  64. doi Zfp143 regulates Nanog through modulation of Oct4 binding
    Xi Chen
    Genome Institute of Singapore, Singapore
    Stem Cells 26:2759-67. 2008
    ..Chromatin immunoprecipitation and electrophoretic mobility shift assays show the direct binding of Zfp143 to the Nanog proximal promoter...
  65. doi Molecular coupling of Xist regulation and pluripotency
    Pablo Navarro
    Institut Pasteur, Unité de Génétique Moléculaire Murine, CNRS, URA2578, F 75015, Paris, France
    Science 321:1693-5. 2008
    ..Here we report that key factors supporting pluripotency-Nanog, Oct3/4, and Sox2-bind within Xist intron 1 in undifferentiated embryonic stem (ES) cells...
  66. doi Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells
    Xiaofei Zhang
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    J Biol Chem 283:35825-33. 2008
    ..program of embryonic stem (ES) cells is orchestrated by a core of transcription factors that has OCT4, SOX2, and NANOG as master regulators...
  67. pmc The C-terminal pentapeptide of Nanog tryptophan repeat domain interacts with Nac1 and regulates stem cell proliferation but not pluripotency
    Tianhua Ma
    Laboratory of Stem Cell Biology, Department of Biological Sciences and Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 284:16071-81. 2009
    Overexpression of Nanog in mouse embryonic stem (ES) cells has been shown to abrogate the requirement of leukemia inhibitory factor for self-renewal in culture. Little is known about the molecular mechanism of Nanog function...
  68. pmc NANOG regulates glioma stem cells and is essential in vivo acting in a cross-functional network with GLI1 and p53
    Marie Zbinden
    Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland
    EMBO J 29:2659-74. 2010
    A cohort of genes associated with embryonic stem (ES) cell behaviour, including NANOG, are expressed in a number of human cancers...
  69. pmc Hedgehog controls neural stem cells through p53-independent regulation of Nanog
    Agnese Po
    Department of Experimental Medicine, Sapienza University, Rome, Italy
    EMBO J 29:2646-58. 2010
    ..The transcription factor Nanog controls stemness acting as a key determinant of both embryonic SC self-renewal and differentiated somatic cells ..
  70. doi The origin and identity of embryonic stem cells
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 138:3-8. 2011
    ..Based on recent findings, we propose here that ES cells can be derived directly from early epiblast cells and that ES cells might arise via two different routes that are dictated by their culture conditions...
  71. pmc The primitive endoderm lineage of the mouse blastocyst: sequential transcription factor activation and regulation of differentiation by Sox17
    Jérôme Artus
    Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Dev Biol 350:393-404. 2011
    ..However, analysis of implantation-delayed blastocysts, revealed a role for SOX17 in the maintenance of PrE epithelial integrity, with the absence of SOX17 leading to premature delamination and migration of parietal endoderm...
  72. pmc Licensing of gametogenesis, dependent on RNA binding protein DAZL, as a gateway to sexual differentiation of fetal germ cells
    Mark E Gill
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 108:7443-8. 2011
    ..In C57BL/6 mice, Dazl is required for licensing. Licensing serves as a gateway from the embryonic processes shared between the sexes--germ cell specification and migration--to the sex-specific pathways of oogenesis and spermatogenesis...
  73. pmc Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells
    Miguel Fidalgo
    Department of Developmental and Regenerative Biology, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York 10029, USA
    Stem Cells 29:1705-16. 2011
    ..properties that are controlled by key components of a core regulatory network including Oct4, Sox2, and Nanog. Understanding molecular underpinnings of these properties requires identification and characterization of ..
  74. doi Primitive endoderm differentiates via a three-step mechanism involving Nanog and RTK signaling
    Stephen Frankenberg
    GReD INSERM U931 CNRS UMR6247 Clermont University, 28 Place Dunant, 63001 Clermont Ferrand, France
    Dev Cell 21:1005-13. 2011
    ..epiblast and the primitive endoderm (PrE)--whose precursors are identifiable by reciprocal expression of Nanog and Gata6, respectively. PrE formation depends on Nanog by a non-cell-autonomous mechanism...
  75. pmc Maternal Cdx2 is dispensable for mouse development
    Stephanie Blij
    Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Development 139:3969-72. 2012
    ..Here, we genetically ablated maternal Cdx2 using a Cre/lox strategy, and we definitively establish that maternal Cdx2 is not essential for mouse development...
  76. pmc OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells
    Pablo Navarro
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland
    EMBO J 31:4547-62. 2012
    b>NANOG, OCT4 and SOX2 form the core network of transcription factors supporting embryonic stem (ES) cell self-renewal...
  77. pmc FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse
    Minjung Kang
    Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Development 140:267-79. 2013
    ..Fgf4 heterozygous blastocysts exhibited increased numbers of NANOG-positive EPI cells and reduced numbers of GATA6-positive PrE cells, suggesting that FGF signaling is tightly ..
  78. pmc Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm
    Irène Aksoy
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore
    EMBO J 32:938-53. 2013
    ..This work provides insights in understanding cell fate transcriptional regulation by highlighting the direct link between the DNA sequence of an enhancer and a developmental outcome...
  79. pmc Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming
    Effie Apostolou
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Cell Stem Cell 12:699-712. 2013
    ..Here, we identified a genome-wide, pluripotency-specific interaction network around the Nanog promoter by adapting circular chromosome conformation capture sequencing...
  80. pmc Oct4 cell-autonomously promotes primitive endoderm development in the mouse blastocyst
    Tristan Frum
    Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Dev Cell 25:610-22. 2013
    ..Finally, we show that Oct4 is required for the expression of multiple EPI and PE genes as well as multiple metabolic pathways essential for the continued growth of the preimplantation embryo. ..
  81. pmc Temporal reduction of LATS kinases in the early preimplantation embryo prevents ICM lineage differentiation
    Chanchao Lorthongpanich
    Mammalian Development Laboratory, Institute of Medical Biology, Singapore
    Genes Dev 27:1441-6. 2013
    ..We therefore conclude that precisely timed YAP localization in early morulae is essential to prevent trophectoderm marker expression in, and lineage specification of, the ICM. ..
  82. pmc Establishment of totipotency does not depend on Oct4A
    Guangming Wu
    Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse 20, 48149 Munster, Germany
    Nat Cell Biol 15:1089-97. 2013
    ..After complete inactivation of both maternal and zygotic Oct4A expression, the embryos still formed Oct4-GFP- and Nanog-expressing inner cell masses, albeit non-pluripotent, indicating that Oct4A is not a determinant for the ..
  83. ncbi Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst
    François Gerbe
    INSERM, UMR384, Faculte de Medecine, 28 place H Dunant BP38, Clermont Ferrand 63001, Universite d Auvergne, Clermont Fd, France
    Dev Biol 313:594-602. 2008
    ..Furthermore, the subcellular location of Lrp2, Disabled-2 (Dab2) and Collagen-IV shows that the epithelial structure is acquired in individual cells through successive steps...
  84. pmc Requirement of Nanog dimerization for stem cell self-renewal and pluripotency
    Jianlong Wang
    Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, and Howard Hughes Medical Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:6326-31. 2008
    ..by transcription factors that form a highly interconnected protein interaction network surrounding the homeobox protein Nanog. Enforced expression of Nanog in mouse ES (mES) cells promotes self-renewal and alleviates their ..
  85. pmc RBM19 is essential for preimplantation development in the mouse
    Jian Zhang
    Division of Gastroenterology, Department of Pediatrics, Medical College of Wisconsin and Children s Research Institute, Milwaukee, WI 53226, USA
    BMC Dev Biol 8:115. 2008
    ..Here we analyzed the role of RBM19 during mouse embryonic development by generating mice containing a mutation in the Rbm19 locus via gene-trap insertion...
  86. ncbi A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal
    Guangjin Pan
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing, China
    FASEB J 20:1730-2. 2006
    ..that ES cells maintain their pluripotent state by expressing a battery of transcription factors including Oct4 and Nanog. However, little is known about how ES cells maintain the expression of these pluripotent factors in ES cells...
  87. pmc Alternative splicing produces Nanog protein variants with different capacities for self-renewal and pluripotency in embryonic stem cells
    Satyabrata Das
    Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 286:42690-703. 2011
    ..The homeodomain transcription factor Nanog acts collaboratively with core factors Oct4 and Sox2 to govern the maintenance of pluripotency...
  88. pmc Sin3a is essential for the genome integrity and viability of pluripotent cells
    Patrick McDonel
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Dev Biol 363:62-73. 2012
    ..These results indicate that Sin3a protects the genomic integrity of pluripotent embryonic cells and governs their unusual cell cycle...
  89. pmc Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis
    Hirotaka Tao
    Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology CDB, Chuo Ku, Kobe, Japan
    Dev Biol 364:138-48. 2012
    ..The cell polarity phenotype was efficiently rescued by nuclear but not cytoplasmic Pk2, demonstrating the nuclear localization of Pk2 is critical for its function...
  90. pmc SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2
    Yongyan Wu
    College of Life Sciences, Northwest A and F University, Yangling, Shaanxi, China
    PLoS ONE 7:e39606. 2012
    b>Nanog is a pivotal transcription factor in embryonic stem (ES) cells and is essential for maintaining the pluripotency and self-renewal of ES cells...
  91. ncbi Determinants of trophoblast lineage and cell subtype specification in the mouse placenta
    David G Simmons
    Department of Biochemistry and Molecular Biology, University of Calgary, HSC Room 2279, 3330 Hospital Drive N W, Calgary, AB, Canada T2N 4N1
    Dev Biol 284:12-24. 2005
    ..Here, we review recent insights into the molecular pathways regulating trophoblast lineage segregation, stem cell maintenance, and subtype differentiation...
  92. ncbi NANOG changes in mouse kidneys with age
    Quan Jian Yan
    Department of Nephrology, Kidney Center and Key Lab of PLA, Chinese General Hospital of PLA, Beijing, PR China
    Rejuvenation Res 8:248-53. 2005
    b>NANOG is essential for mouse and human embryonic stem cell (ESC) pluripotency and selfrenewal. It is also expressed in several adult murine tissues as shown by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis...
  93. ncbi Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    ..Here we show that selection for Nanog expression results in germline-competent iPS cells with increased ES-cell-like gene expression and DNA methylation ..
  94. ncbi BMP signalling inhibits premature neural differentiation in the mouse embryo
    Aida Di-Gregorio
    Molecular Embryology Group, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 ONN, UK
    Development 134:3359-69. 2007
    ..Together, our results demonstrate that inhibition of BMP signalling has a central role during neural induction in mammals and suggest that FGFs do not act as neural inducers in the post-implantation mouse embryo...
  95. pmc Detailed characterization of the mouse embryonic stem cell transcriptome reveals novel genes and intergenic splicing associated with pluripotency
    Galih Kunarso
    Computational and Mathematical Biology, Genome Institute of Singapore, 60 Biopolis Street 02 01, Singapore 138672, Singapore
    BMC Genomics 9:155. 2008
    ..Transcriptional regulators including Oct4 (Oct3/4 index), Sox2 and Nanog are fundamental for maintaining the undifferentiated state...
  96. doi Hepatoblast-like cells enriched from mouse embryonic stem cells in medium without glucose, pyruvate, arginine, and tyrosine
    Minoru Tomizawa
    Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba City, Chiba, 260 8670, Japan
    Cell Tissue Res 333:17-27. 2008
    ..5 days of gestation) and expression of C/EBPbeta at a similar level to that of fetal liver. These data support our conclusion that HSM allows the selection of hepatoblast-like cells...
  97. doi Heterogeneity of embryonic and adult stem cells
    Thomas Graf
    Center for Genomic Regulation, ICREA and Pompeu Fabra University, 08003 Barcelona, Spain
    Cell Stem Cell 3:480-3. 2008
    ..These cell states show a bias in their differentiation potential and correlate with specific patterns of transcription factor expression and chromatin modifications...
  98. doi Role for Med12 in regulation of Nanog and Nanog target genes
    Antonin V Tutter
    Epigenetics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts 02139, USA
    J Biol Chem 284:3709-18. 2009
    b>Nanog, Oct4, and Sox2 form the core of a transcription factor network that maintains embryonic stem cells in the pluripotent state in both humans and mice...
  99. pmc Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4
    Costas A Lyssiotis
    Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:8912-7. 2009
    ..A series of small-molecule scaffolds were identified that activate Nanog expression in mouse fibroblasts transduced with a subset of reprogramming factors lacking Klf4...
  100. doi Protein chip analysis of pluripotency-associated proteins in NIH3T3 fibroblast
    Brijesh S Ajjappala
    Department of Biomedical Sciences, CHA Stem Cell Institute, CHA University, CHA General Hospital, Seoul 135 081, Korea
    Proteomics 9:3968-78. 2009
    ..Amongst these regulatory transcription factors in embryonic stem cells, Oct4 and Nanog are master factors that also have unique characteristic ability of cell-specific pluripotency and self-renewal...
  101. pmc Impairment of developmental stem cell-mediated striatal neurogenesis and pluripotency genes in a knock-in model of Huntington's disease
    Aldrin E Molero
    Institute for Brain Disorders and Neural Regeneration, and Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 106:21900-5. 2009
    ..Further, our analysis revealed that in addition to Sox2, the core PP factor, Nanog is expressed within the striatal generative and mantle regions, and in Hdh-Q111 embryos the fraction of Nanog-..