Ldb1

Summary

Gene Symbol: Ldb1
Description: LIM domain binding 1
Alias: CLIM2, NLI, LIM domain-binding protein 1, CLIM-2, LDB-1, LIM domain-binding factor CLIM2, carboxyl-terminal LIM domain-binding protein 2, mLdb1, nuclear LIM interactor
Species: mouse

Top Publications

  1. ncbi Interactions of the LIM-domain-binding factor Ldb1 with LIM homeodomain proteins
    A D Agulnick
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 384:270-2. 1996
  2. ncbi A family of LIM domain-associated cofactors confer transcriptional synergism between LIM and Otx homeodomain proteins
    I Bach
    Howard Hughes Medical Institute, Eukaryotic Regulatory Biology Program, University of California, San Diego, School of Medicine, La Jolla 92093 0648, USA
    Genes Dev 11:1370-80. 1997
  3. ncbi The expression and stage-specific localization of protein kinase C isotypes during mouse preimplantation development
    C M Pauken
    Molecular and Cellular Biology Program, Department of Biology, Arizona State University, Tempe, Arizona, 85287 1501, USA
    Dev Biol 223:411-21. 2000
  4. pmc LIM-homeodomain proteins Lhx1 and Lhx5, and their cofactor Ldb1, control Purkinje cell differentiation in the developing cerebellum
    Yangu Zhao
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2790, USA
    Proc Natl Acad Sci U S A 104:13182-6. 2007
  5. doi The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences
    Minyoung Hwang
    Graduate School of Medicine, Korea University, Seoul, Korea
    Stem Cells 26:1490-5. 2008
  6. pmc Co-factors of LIM domains (Clims/Ldb/Nli) regulate corneal homeostasis and maintenance of hair follicle stem cells
    Xiaoman Xu
    Department of Medicine, Division of Endocrinology, Sprague Hall, Room 206, University of California, Irvine, Irvine, CA 92697 4030, USA
    Dev Biol 312:484-500. 2007
  7. ncbi The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes
    Hani Suleiman
    University of Regensburg, Institute for Molecular and Cellular Anatomy, Universitätsstr 31, 93053 Regensburg, and Division of Nephrology and Immunology, University Hospital, RWTH, Aachen, Germany
    Dev Biol 304:701-12. 2007
  8. pmc Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes
    Mugdha Bhati
    School of Molecular and Microbial Biosciences, The University of Sydney, New South Wales, Australia
    EMBO J 27:2018-29. 2008
  9. pmc SCL and associated proteins distinguish active from repressive GATA transcription factor complexes
    Tamara Tripic
    Division of Hematology, The Children s Hospital of Philadelphia, PA 19104, USA
    Blood 113:2191-201. 2009
  10. pmc LMO4 controls the balance between excitatory and inhibitory spinal V2 interneurons
    Kaumudi Joshi
    Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Neuron 61:839-51. 2009

Research Grants

  1. Genome-wide location analysis of neuronal genes
    Michael Rosenfeld; Fiscal Year: 2007

Scientific Experts

  • Michael Rosenfeld
  • Juhee Jeong
  • Yangu Zhao
  • Ginat Narkis
  • Sang Hyun Song
  • Andreas Teufel
  • A D Agulnick
  • I Bach
  • Mi Ryoung Song
  • Marten P Smidt
  • Heiner Westphal
  • Liqi Li
  • Frank Grosveld
  • Ann Dean
  • Marat Gorivodsky
  • Mugdha Bhati
  • Paul E Love
  • Athina Mylona
  • Chad S Hunter
  • Eric Soler
  • Wulan Deng
  • Jacqueline M Matthews
  • Joel P Mackay
  • Zhixiong Xu
  • Soojin Kim
  • Jamie R Galloway
  • Noah Spies
  • Feng Zhang
  • Luca Caputo
  • Evgeny Makarev
  • Sally H Cross
  • Asma Almaidhan
  • Ivan Krivega
  • Stephen J Brandt
  • Patrick Rodriguez
  • Mirna Marinić
  • Wilfred van Ijcken
  • Boris Lenhard
  • Supat Thongjuea
  • Charlotte Andrieu-Soler
  • Mahua Mukhopadhyay
  • Christel Kockx
  • Siavoush Dastmalchi
  • Keji Zhao
  • Anandi Bhattacharya
  • Raja Jothi
  • Dongho Geum
  • Tsadok Cohen
  • Emma Juuri
  • Ralph Stadhouders
  • Kairong Cui
  • Joanna S T Asprer
  • Jan Y Lee
  • Gerd A Blobel
  • Snezana Kojic
  • Soo Kyung Lee
  • Christopher Lee
  • Bora Lee
  • Chris J Storbeck
  • Tamara Tripic
  • Kaumudi Joshi
  • Ipsita Dey-Guha
  • Kenneth A Ohgi
  • Chijen Lin
  • Ernie de Boer
  • Mira T Kassouf
  • Ying Cai
  • Yong Chao Ma
  • Josette Renee Landry
  • Minyoung Hwang
  • Xiaoman Xu
  • Hani Suleiman
  • Paresh Vyas
  • Yen Ha Tran
  • Natalia Meier
  • John Strouboulis
  • Jeroen Krijgsveld
  • Noriyuki Nishioka
  • Kenneth J Woodside
  • Woon Ryoung Kim
  • Ja Myong Lee
  • Sarada Bulchand
  • Qing Zhang
  • L W Jurata
  • Laura Buttitta
  • Yuliang Tan
  • Cheryl L Smith
  • Arend Sidow
  • Jesse M Rodriguez
  • Serafim Batzoglou

Detail Information

Publications71

  1. ncbi Interactions of the LIM-domain-binding factor Ldb1 with LIM homeodomain proteins
    A D Agulnick
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 384:270-2. 1996
    ..How LIM domains exert these regulatory effects is not known. We have now isolated a new LIM-domain-binding factor, Ldb1, on the basis of its ability to interact with the LIM-HD protein Lhx1 (Lim1)...
  2. ncbi A family of LIM domain-associated cofactors confer transcriptional synergism between LIM and Otx homeodomain proteins
    I Bach
    Howard Hughes Medical Institute, Eukaryotic Regulatory Biology Program, University of California, San Diego, School of Medicine, La Jolla 92093 0648, USA
    Genes Dev 11:1370-80. 1997
    ..Our results link LIM homeodomain proteins and members of the Otx class of transcription factors in gene activation events during embryogenesis via the actions of specific cofactors...
  3. ncbi The expression and stage-specific localization of protein kinase C isotypes during mouse preimplantation development
    C M Pauken
    Molecular and Cellular Biology Program, Department of Biology, Arizona State University, Tempe, Arizona, 85287 1501, USA
    Dev Biol 223:411-21. 2000
    ..Two isotypes have a stage-dependent nuclear localization. In addition, within each blastomere PKC isotypes occupy different subcellular locations in a stage-dependent fashion...
  4. pmc LIM-homeodomain proteins Lhx1 and Lhx5, and their cofactor Ldb1, control Purkinje cell differentiation in the developing cerebellum
    Yangu Zhao
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2790, USA
    Proc Natl Acad Sci U S A 104:13182-6. 2007
    ..In addition, targeted inactivation of Ldb1, which encodes a cofactor for all LIM-homeodomain proteins, resulted in a similar phenotype...
  5. doi The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences
    Minyoung Hwang
    Graduate School of Medicine, Korea University, Seoul, Korea
    Stem Cells 26:1490-5. 2008
    LIM-domain binding protein 1 (Ldb1) is a multiadaptor protein that mediates the action of transcription factors, including LIM-homeodomain proteins...
  6. pmc Co-factors of LIM domains (Clims/Ldb/Nli) regulate corneal homeostasis and maintenance of hair follicle stem cells
    Xiaoman Xu
    Department of Medicine, Division of Endocrinology, Sprague Hall, Room 206, University of California, Irvine, Irvine, CA 92697 4030, USA
    Dev Biol 312:484-500. 2007
    ..In addition, Clim2 interacts with Lhx2 in vivo, suggesting that Clim2 is an essential co-factor for the LIM homeodomain factor Lhx2, ..
  7. ncbi The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes
    Hani Suleiman
    University of Regensburg, Institute for Molecular and Cellular Anatomy, Universitätsstr 31, 93053 Regensburg, and Division of Nephrology and Immunology, University Hospital, RWTH, Aachen, Germany
    Dev Biol 304:701-12. 2007
    ..Promising candidates for modifier proteins are the proteins interacting with LMX1B, such as LDB1 and E47...
  8. pmc Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes
    Mugdha Bhati
    School of Molecular and Microbial Biosciences, The University of Sydney, New South Wales, Australia
    EMBO J 27:2018-29. 2008
    ..In the ventral spinal cord, the binary LIM homeobox protein 3 (Lhx3)/LIM domain-binding protein 1 (Ldb1) complex specifies the formation of V2 interneurons...
  9. pmc SCL and associated proteins distinguish active from repressive GATA transcription factor complexes
    Tamara Tripic
    Division of Hematology, The Children s Hospital of Philadelphia, PA 19104, USA
    Blood 113:2191-201. 2009
    ..a conditional erythroid rescue system as well as through the use of tiling arrays we detected the SCL/TAL1, LMO2, Ldb1, E2A complex at all positively acting GATA-1-bound elements examined...
  10. pmc LMO4 controls the balance between excitatory and inhibitory spinal V2 interneurons
    Kaumudi Joshi
    Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Neuron 61:839-51. 2009
    ..We further demonstrate that LMO4 nucleates the assembly of a novel LIM-complex containing SCL, Gata2, and NLI. This complex activates specific enhancers in V2b-genes consisting of binding sites for SCL and Gata2, thereby ..
  11. pmc Role of ldb1 in adult intestinal homeostasis
    Ipsita Dey-Guha
    Laboratory of Mammalian Genes and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, HHS, Bethesda, MD 20892, USA
    Int J Biol Sci 5:686-94. 2009
    b>Ldb1 is an essential co-regulator of transcription in embryonic development. It acts in conjunction with nuclear LIM-homeodomain and LIM-only proteins to control key events of organogenesis as precursor cells enter lineage specification...
  12. pmc Multiple functions of Ldb1 required for beta-globin activation during erythroid differentiation
    Sang Hyun Song
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 116:2356-64. 2010
    b>Ldb1 and erythroid partners SCL, GATA-1, and LMO2 form a complex that is required to establish spatial proximity between the β-globin locus control region and gene and for transcription activation during erythroid differentiation...
  13. doi Liver-specific Ldb1 deletion results in enhanced liver cancer development
    Andreas Teufel
    Department of Medicine I, Johannes Gutenberg University, Mainz, Germany
    J Hepatol 53:1078-84. 2010
    ..We have previously demonstrated Ldb1 to be of high biological and developmental relevance, as a targeted deletion of the Ldb1 gene in mice results in an ..
  14. pmc Identification of a TAL1 target gene reveals a positive role for the LIM domain-binding protein Ldb1 in erythroid gene expression and differentiation
    Zhixiong Xu
    Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    Mol Cell Biol 23:7585-99. 2003
    ..partner E47, zinc finger transcription factor GATA-1, LIM domain protein LMO2, and LIM domain-binding protein Ldb1 transactivates the protein 4.2 (P4.2) gene through two E box GATA elements in its proximal promoter...
  15. pmc Nuclear adaptor Ldb1 regulates a transcriptional program essential for the maintenance of hematopoietic stem cells
    Liqi Li
    Section on Cellular and Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Nat Immunol 12:129-36. 2011
    The nuclear adaptor Ldb1 functions as a core component of multiprotein transcription complexes that regulate differentiation in diverse cell types...
  16. pmc LMO4 functions as a co-activator of neurogenin 2 in the developing cortex
    Joanna S T Asprer
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Development 138:2823-32. 2011
    ..LMO4 and its binding partner nuclear LIM interactor (NLI/LDB1/CLIM2) interact with NGN2 simultaneously, forming a multi-protein transcription complex...
  17. pmc Isl1 and Ldb co-regulators of transcription are essential early determinants of mouse limb development
    Ginat Narkis
    Laboratory of Mammalian Genes and Development, Program in Genomics of Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Dyn 241:787-91. 2012
    ..However, details regarding the control of limb initiation and early stages of outgrowth remain to be defined. This report is focused on genetic events that pave the way for the establishment of a hindlimb bud...
  18. ncbi CNS expression pattern of Lmx1b and coexpression with ptx genes suggest functional cooperativity in the development of forebrain motor control systems
    Ceriel H J Asbreuk
    Rudolf Magnus Institute of Neuroscience, Department of Pharmacology and Anatomy, University Medical Center, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Mol Cell Neurosci 21:410-20. 2002
    ..In addition, the mutual cofactor Ldb1 is expressed in these regions. The expression of these gene sets is maintained in the adult brain...
  19. pmc Functional analysis of the nuclear LIM domain interactor NLI
    L W Jurata
    Biomedical Sciences Graduate Program, University of California at San Diego, La Jolla 92093 0650, USA
    Mol Cell Biol 17:5688-98. 1997
    ..A recently identified, widely expressed protein, NLI, binds with high affinity to the LIM domains of LIM homeodomain and LMO proteins in vitro and in vivo...
  20. pmc The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation
    J E Visvader
    Division of Hematology Oncology, Children s Hospital and the Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:13707-12. 1997
    ..We, and others, have identified a widely expressed protein, Ldb1, whose C-terminal 76-residues are sufficient to mediate interaction with LMO2...
  21. pmc Nuclear LIM interactor, a rhombotin and LIM homeodomain interacting protein, is expressed early in neuronal development
    L W Jurata
    Biomedical Sciences Graduate Program, University of California at San Diego, La Jolla 92093 0650, USA
    Proc Natl Acad Sci U S A 93:11693-8. 1996
    ..We have isolated a novel protein, nuclear LIM interactor (NLI), that specifically associates with a single LIM domain in all nuclear LIM proteins tested...
  22. ncbi Conserved regulation of mesenchymal gene expression by Fgf-8 in face and limb development
    A S Tucker
    Department of Craniofacial Development, GKT Dental Institute, Kings College, Guy s Hospital, London SE1 9RT, UK
    Development 126:221-8. 1999
    Clim-2 (NLI, Lbd1) is one of two related mouse proteins that interact with Lim-domain homeoproteins...
  23. pmc Mouse deformed epidermal autoregulatory factor 1 recruits a LIM domain factor, LMO-4, and CLIM coregulators
    T M Sugihara
    Division of Endocrinology and Metabolism, Department and School of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 95:15418-23. 1998
    Nuclear LIM domains interact with a family of coregulators referred to as Clim/Ldb/Nli. Although one family member, Clim-2/Ldb-1/Nli, is highly expressed in epidermal keratinocytes, no nuclear LIM domain factor is known to be expressed ..
  24. ncbi Functional ablation of the mouse Ldb1 gene results in severe patterning defects during gastrulation
    Mahua Mukhopadhyay
    Department of Anatomy, University of Wisconsin Madison Medical School, Madison, WI 53706, USA
    Development 130:495-505. 2003
    The LIM domain-binding protein 1 (Ldb1) is found in multi-protein complexes containing various combinations of LIM-homeodomain, LIM-only, bHLH, GATA and Otx transcription factors. These proteins exert key functions during embryogenesis...
  25. pmc Constraint and divergence of global gene expression in the mammalian embryo
    Noah Spies
    Department of Pathology, Stanford University School of Medicine, Stanford, United States
    elife 4:e05538. 2015
    ..The widespread effect of maternal and embryonic genotype in conjunction with the purifying selection we uncovered suggests that embryogenesis is an important and understudied reservoir of phenotypic variation. ..
  26. pmc Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program
    Feng Zhang
    Howard Hughes Medical Institute and Department of Medicine, School of Medicine
    Proc Natl Acad Sci U S A 112:1380-5. 2015
    ..report the required actions of LIM domain-binding protein 1 (LDB1)/cofactor of LIM homeodomain protein 2/nuclear LIM interactor, interacting with the enhancer-binding protein achaete-scute complex homolog 1, to mediate looping to ..
  27. pmc Role of LDB1 in the transition from chromatin looping to transcription activation
    Ivan Krivega
    Laboratory of Cellular and Developmental Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 28:1278-90. 2014
    ..In erythroid cells, lim domain binding 1 (LDB1) protein is recruited to the β-globin locus via LMO2 and is required for looping of the β-globin locus control ..
  28. doi The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors
    Luca Caputo
    Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany
    Cell Stem Cell 17:287-99. 2015
    ..Here we show that Ldb1 is a central regulator of genome organization in cardiac progenitor cells, which is crucial for cardiac lineage ..
  29. pmc SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic β-Cell Target Genes
    Jamie R Galloway
    Department of Medicine J R G, M B, Y L, R U, C S H, Division of Endocrinology, Diabetes and Metabolism, and Comprehensive Diabetes Center, and Department of Surgery J A M, University of Alabama at Birmingham, Birmingham, Alabama 35294
    Mol Endocrinol 29:1774-86. 2015
    ..development, maturation, and function, which largely requires interaction with the LIM domain-binding protein 1 (Ldb1) coregulator...
  30. pmc A dominant-negative mutation of mouse Lmx1b causes glaucoma and is semi-lethal via LDB1-mediated dimerization [corrected]
    Sally H Cross
    MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Genet 10:e1004359. 2014
    ..Co-immunoprecipitation experiments show that both wild-type and Icst LMX1B are found in complexes with LIM domain binding protein 1 (LDB1), resulting in lower levels of functional LMX1B in Icst heterozygotes than null heterozygotes...
  31. pmc Islet1 and its co-factor Ldb1 are expressed in quiescent cells of mouse intestinal epithelium
    Evgeny Makarev
    Section on Mammalian Molecular Genetics, Laboratory of Mammalian Genes and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, United States of America
    PLoS ONE 9:e95256. 2014
    ..Isl1 (Islet1), Lhx and Ldb1 genes play a crucial role in organ patterning, cell fate determination and cell differentiation in both embryonic ..
  32. pmc Neural crest-specific deletion of Ldb1 leads to cleft secondary palate with impaired palatal shelf elevation
    Asma Almaidhan
    Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 10010 New York, NY, USA
    BMC Dev Biol 14:3. 2014
    b>LIM domain binding protein 1 (LDB1) is a transcriptional co-factor, which interacts with multiple transcription factors and other proteins containing LIM domains...
  33. pmc Ldb1 is essential for development of Nkx2.1 lineage derived GABAergic and cholinergic neurons in the telencephalon
    Yangu Zhao
    Program on Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA Electronic address
    Dev Biol 385:94-106. 2014
    ..Here we investigate the role of Ldb1, a nuclear protein that binds directly to all LIM-HD factors, in the development of these ventral telencephalon ..
  34. pmc Regulation of motor neuron specification by phosphorylation of neurogenin 2
    Yong Chao Ma
    F M Kirby Neurobiology Center, Children s Hospital, and Departments of Neurology and Neurobiology, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    Neuron 58:65-77. 2008
    ....
  35. pmc Ldb1 Is Essential for the Development of Isthmic Organizer and Midbrain Dopaminergic Neurons
    Soojin Kim
    1 Department of Biomedical Sciences, Korea University Medical School, Seoul, South Korea
    Stem Cells Dev 25:986-94. 2016
    LIM domain-binding protein 1 (Ldb1) is a nuclear cofactor that interacts with LIM homeodomain proteins to form multiprotein complexes that are important for transcription regulation...
  36. pmc Ldb1-nucleated transcription complexes function as primary mediators of global erythroid gene activation
    Liqi Li
    Section on Cellular and Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 121:4575-85. 2013
    ..Gata1 and Tal1 can assemble within higher-order protein complexes (Ldb1 complexes) that include the adapter molecules Lmo2 and Ldb1...
  37. doi An integrated holo-enhancer unit defines tissue and gene specificity of the Fgf8 regulatory landscape
    Mirna Marinić
    Developmental Biology Unit, EMBL, Meyerhofstrasse 1, Heidelberg 69117, Germany
    Dev Cell 24:530-42. 2013
    ..We discuss the implications of such regulatory systems regarding the evolution of gene expression and the impact of human genomic structural variations...
  38. doi Genome-wide analysis shows that Ldb1 controls essential hematopoietic genes/pathways in mouse early development and reveals novel players in hematopoiesis
    Athina Mylona
    Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands
    Blood 121:2902-13. 2013
    ..molecular and functional studies in blast colony-forming cells to gain insight into the function of the essential Ldb1 factor in early primitive hematopoietic development...
  39. pmc Upstream distal regulatory elements contact the Lmo2 promoter in mouse erythroid cells
    Anandi Bhattacharya
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 7:e52880. 2012
    ..Furthermore, these chromatin loops are supported by the cohesin complex recruited to both CTCF and transcription factor bound regions...
  40. pmc Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions
    Mugdha Bhati
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 7:e40719. 2012
    ..The recently published structures of Lhx3 in complex with each of two key protein partners, Isl1 and Ldb1, provide an opportunity to understand the effect of mutations and posttranslational modifications on key protein-..
  41. pmc Sox2+ stem cells contribute to all epithelial lineages of the tooth via Sfrp5+ progenitors
    Emma Juuri
    Developmental Biology Program, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland
    Dev Cell 23:317-28. 2012
    ..The identification of Sox2 as a marker for the dental epithelial stem cells will facilitate further studies on their lineage segregation and differentiation during tooth renewal...
  42. pmc Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development
    Ralph Stadhouders
    Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 31:986-99. 2012
    ..We propose a model for Myb activation by distal enhancers dynamically bound by KLF1 and the GATA1/TAL1/LDB1 complex, which primarily function as a transcription elongation element through chromatin looping.
  43. pmc A requirement for Lim domain binding protein 1 in erythropoiesis
    Liqi Li
    Section on Cellular and Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 207:2543-50. 2010
    During erythrocyte development, the nuclear cofactor Lim domain binding protein 1 (Ldb1) functions as a core subunit of multiprotein DNA binding complexes that include the transcription factors Scl and Gata-1 and the Lim-only adapter Lmo2...
  44. doi Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis
    Mira T Kassouf
    Department of Haematology, Medical Research Council, Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
    Blood 112:1056-67. 2008
    ....
  45. pmc Islet α-, β-, and δ-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor
    Chad S Hunter
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Diabetes 62:875-86. 2013
    b>Ldb1 and Ldb2 are coregulators that mediate Lin11-Isl1-Mec3 (LIM)-homeodomain (HD) and LIM-only transcription factor-driven gene regulation...
  46. pmc Solution structure of a tethered Lmo2(LIM2) /Ldb1(LID) complex
    Siavoush Dastmalchi
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales 2006, Australia
    Protein Sci 21:1768-74. 2012
    ..Lmo2 interacts with LIM domain binding protein 1 (Ldb1) through the tandem LIM domains of Lmo2 and the LIM interaction domain (LID) of Ldb1...
  47. pmc Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor
    Wulan Deng
    Division of Hematology, The Children s Hospital of Philadelphia, PA 19104, USA
    Cell 149:1233-44. 2012
    ..and β-globin promoter form a chromatin loop that requires transcription factor GATA1 and the associated molecule Ldb1. We employed artificial zinc fingers (ZF) to tether Ldb1 to the β-globin promoter in GATA1 null erythroblasts, in ..
  48. pmc Ldb1 regulates carbonic anhydrase 1 during erythroid differentiation
    Sang Hyun Song
    Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
    Biochim Biophys Acta 1819:885-91. 2012
    ..Here, we show that Car1 mRNA decreases significantly when erythroid differentiation is induced in MEL cells. The Ldb1 protein complex including GATA1/SCL/LMO2 binds to the Car1 promoter in uninduced cells and reduced enrichment of ..
  49. pmc GATA-1 forms distinct activating and repressive complexes in erythroid cells
    Patrick Rodriguez
    Department of Cell Biology, Erasmus University Medical Center, Rotterdam, The Netherlands
    EMBO J 24:2354-66. 2005
    ..Based on these findings, we suggest a model for the different roles of GATA-1 in erythroid differentiation...
  50. pmc Single-stranded DNA-binding proteins regulate the abundance and function of the LIM-homeodomain transcription factor LHX2 in pituitary cells
    Ying Cai
    Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 373:303-8. 2008
    ..LIM-homeodomain (LIM-HD) transcription factors in embryogenesis by interaction with LIM domain-binding protein-1 (LDB1)...
  51. ncbi Interactions between LIM domains and the LIM domain-binding protein Ldb1
    J J Breen
    Laboratory of Molecular Genetics, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:4712-7. 1998
    ..The recently described protein Ldb1 (also known as NLI; LIM domain-binding protein) binds LIM domains in vitro and synergizes with the LIM-homeodomain ..
  52. ncbi Genomic structure and chromosomal localization of the mouse LIM domain-binding protein 1 gene, Ldb1
    T Yamashita
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2790, USA
    Genomics 48:87-92. 1998
    ..Here we report on the structural organization and chromosomal localization of the mouse Ldb1 gene. It contains at least 10 exons and spans approximately 4 kb of genomic DNA...
  53. ncbi Cloning and chromosomal localization of two novel human genes encoding LIM-domain binding factors CLIM1 and CLIM2/LDB1/NLI
    E V Semina
    Department of Pediatrics, The University of Iowa, 200 Hawkins Drive, W229 1 GH, Iowa City, Iowa 52242 1083, USA
    Mamm Genome 9:921-4. 1998
  54. ncbi A brain region-specific gene product Lhx6.1 interacts with Ldb1 through tandem LIM-domains
    N Kimura
    Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 101 0062, Japan
    J Biochem 126:180-7. 1999
    ..activity of several LHX proteins is postulated to be regulated by interaction with an LIM-domain binding protein, Ldb1. We have now identified a novel LHX molecule, termed Lhx6...
  55. ncbi RLIM inhibits functional activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex
    I Bach
    Howard Hughes Medical Institute, Eukaryotic Regulatory Biology Program, University of California, San Diego, School of Medicine, La Jolla 92093 0648, USA
    Nat Genet 22:394-9. 1999
    ..The inhibitory actions of the LIM domain can often be overcome by the LIM co-regulator known as CLIM2, LDB1 and NLI (referred to hereafter as CLIM2; refs 2-4)...
  56. ncbi LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem
    S Bulchand
    Department of Biological Sciences, Tata Institute of Fundamental Research, 400005, Mumbai, India
    Mech Dev 100:165-75. 2001
    ..The defect in the Lhx2-/- telencephalon appears to be at this step...
  57. ncbi A double-deletion mutation in the Pitx3 gene causes arrested lens development in aphakia mice
    D K Rieger
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts, 02115, USA
    Genomics 72:61-72. 2001
    ..We conclude that the ak mutation consists of two deletions, the larger of which removes part of Pitx3, indicating a crucial role of this gene in early lens development...
  58. ncbi Ubiquitination-dependent cofactor exchange on LIM homeodomain transcription factors
    Heather P Ostendorff
    Zentrum für Molekulare Neurobiologie Hamburg ZMNH, Universitat Hamburg, Martinistrasse 85, 20251 Hamburg, Germany
    Nature 416:99-103. 2002
    ..with RLIM (RING finger LIM domain-binding protein) and with CLIM (cofactor of LIM-HD proteins; also known as NLI, Ldb and Chip) cofactors...
  59. ncbi Identification of a Wnt/Dvl/beta-Catenin --> Pitx2 pathway mediating cell-type-specific proliferation during development
    Chrissa Kioussi
    Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, CMM West, Room 345, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cell 111:673-85. 2002
    ....
  60. doi Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver
    Josette Renee Landry
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
    Blood 111:3005-14. 2008
    ..Together, our data provide a key component of the transcriptional network of early hematopoiesis by identifying downstream targets of Scl that can explain key aspects of the early Scl(-/-) phenotype...
  61. ncbi Microarray analysis of somitogenesis reveals novel targets of different WNT signaling pathways in the somitic mesoderm
    Laura Buttitta
    Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210, USA
    Dev Biol 258:91-104. 2003
    ....
  62. ncbi Novel binding partners of Ldb1 are required for haematopoietic development
    Natalia Meier
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 2040, 3000 CA, Rotterdam, The Netherlands
    Development 133:4913-23. 2006
    b>Ldb1, a ubiquitously expressed LIM domain binding protein, is essential in a number of tissues during development. It interacts with Gata1, Tal1, E2A and Lmo2 to form a transcription factor complex regulating late erythroid genes...
  63. ncbi Spliced isoforms of LIM-domain-binding protein (CLIM/NLI/Ldb) lacking the LIM-interaction domain
    Yen Ha Tran
    Department of Biological Sciences, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501
    J Biochem 140:105-19. 2006
    ..Here, we demonstrate that splice isoforms lacking the LID are also present in the Ldb1 genes of mammals, chick, and Xenopus, as well as in fish paralog Ldb4...
  64. pmc Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity
    Mi Ryoung Song
    Bioimaging Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea
    Development 136:2923-32. 2009
    LIM transcription factors bind to nuclear LIM interactor (Ldb/NLI/Clim) in specific ratios to form higher-order complexes that regulate gene expression...
  65. pmc The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration
    Chris J Storbeck
    Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
    Mol Biol Cell 20:4174-82. 2009
    ..SLK, required for cell migration, interacts with the LIM domain binding transcriptional cofactor proteins Ldb1/CLIM2 and Ldb2/CLIM1/NLI...
  66. ncbi Ssdp1 regulates head morphogenesis of mouse embryos by activating the Lim1-Ldb1 complex
    Noriyuki Nishioka
    Laboratory for Embryonic Induction, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Development 132:2535-46. 2005
    ..of single-stranded DNA-binding protein 1 (Ssdp1), which encodes a co-factor of LIM domain interacting protein 1 (Ldb1), in the mouse mutant headshrinker (hsk) disrupts anterior head development by partially mimicking Lim1 mutants...
  67. ncbi Expression of Dlx and Lhx family homeobox genes in fetal thymus and thymocytes
    Kenneth J Woodside
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Building 6B, Room 2B 210, 9000 Rockville Pike, Bethesda, MD 20892 2780, USA
    Gene Expr Patterns 4:315-20. 2004
    ....
  68. doi A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein
    Snezana Kojic
    Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, Serbia
    Arch Biochem Biophys 502:60-7. 2010
    ..It is probable that Ankrd1/CARP has a role in the propagation of signals initiated by myogenic regulatory factors (MRFs) during myogenesis...
  69. ncbi Ru2 and Ru encode mouse orthologs of the genes mutated in human Hermansky-Pudlak syndrome types 5 and 6
    Qing Zhang
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Nat Genet 33:145-53. 2003
    ..Both genes are previously unknown and are found only in higher eukaryotes, and together represent a new class of genes that have evolved in higher organisms to govern the synthesis of highly specialized lysosome-related organelles...
  70. ncbi Early developmental failure of substantia nigra dopamine neurons in mice lacking the homeodomain gene Pitx3
    Marten P Smidt
    Rudolf Magnus Institute of Neuroscience, Department of Pharmacology and Anatomy, University Medical Center, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Development 131:1145-55. 2004
    ..However, an overall lower activity was observed. The results suggest that Pitx3 is specifically required for the formation of the SNc subfield at the onset of dopaminergic neuron differentiation...
  71. ncbi Dynamic spatiotemporal expression of LIM genes and cofactors in the embryonic and postnatal cerebral cortex
    Sarada Bulchand
    Tata Institute of Fundamental Research, Mumbai, India
    Dev Dyn 226:460-9. 2003
    ..These results suggest multiple and distinct roles for LIM-HD, Lmo, and Clim genes in cortical development, and also support a LIM-HD-independent role for some Lmo and Clim members...

Research Grants1

  1. Genome-wide location analysis of neuronal genes
    Michael Rosenfeld; Fiscal Year: 2007
    ..We believe that with validation of these approaches, technologies developed here will be of widespread utility in neuroscience, particularly in studies of development and neurodegenerative disease. ..