Dgat1

Summary

Gene Symbol: Dgat1
Description: diacylglycerol O-acyltransferase 1
Alias: ARAT, C75990, D15Ertd23e, Dgat, diacylglycerol O-acyltransferase 1, acyl-CoA retinol O-fatty-acyltransferase, diglyceride acyltransferase
Species: mouse

Top Publications

  1. pmc Effects of DGAT1 deficiency on energy and glucose metabolism are independent of adiponectin
    Ryan S Streeper
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Am J Physiol Endocrinol Metab 291:E388-94. 2006
  2. pmc Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
    Chi Liang Eric Yen
    Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53706, USA
    J Lipid Res 49:2283-301. 2008
  3. ncbi Role of adipocyte-derived factors in enhancing insulin signaling in skeletal muscle and white adipose tissue of mice lacking Acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    James A Haley Veterans Hospital, ACOS 151, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USA
    Diabetes 53:1445-51. 2004
  4. ncbi Increased very low density lipoprotein secretion and gonadal fat mass in mice overexpressing liver DGAT1
    Tomomi Yamazaki
    Division of Clinical Nutrition, National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    J Biol Chem 280:21506-14. 2005
  5. ncbi The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94158, USA
    J Lipid Res 46:1502-11. 2005
  6. ncbi Whole-body insulin resistance in the absence of obesity in FVB mice with overexpression of Dgat1 in adipose tissue
    Nancy Chen
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 West 168th St, PH 10 305J, New York, New York 10032, USA
    Diabetes 54:3379-86. 2005
  7. ncbi Short-term overexpression of DGAT1 or DGAT2 increases hepatic triglyceride but not VLDL triglyceride or apoB production
    John S Millar
    Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, USA
    J Lipid Res 47:2297-305. 2006
  8. pmc Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance
    Li Liu
    Department of Medicine, Columbia University, New York, New York 10032, USA
    J Clin Invest 117:1679-89. 2007
  9. doi Knockdown of acyl-CoA:diacylglycerol acyltransferase 2 with antisense oligonucleotide reduces VLDL TG and ApoB secretion in mice
    Ying Liu
    Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA 19104 6160, USA
    Biochim Biophys Acta 1781:97-104. 2008
  10. pmc The molecular basis of retinoid absorption: a genetic dissection
    Nuttaporn Wongsiriroj
    Institute of Human Nutrition and Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 283:13510-9. 2008

Research Grants

Scientific Experts

  • Alan M Birch
  • Kimberly K Buhman
  • Qin Liu
  • Yi Hao Yu
  • Carrie L Welch
  • J S Millar
  • I J Waterman
  • Gouri Ranganathan
  • Jingsong Cao
  • Steven J Y Wang
  • Dong Cheng
  • Suneil K Koliwad
  • Chi Liang Eric Yen
  • DENIS J GLENN
  • Charles A Harris
  • Robert V Farese
  • Li Liu
  • Hubert C Chen
  • Ira J Goldberg
  • Ryan S Streeper
  • Ping Zhou
  • Scot J Stone
  • William S Blaner
  • Nuttaporn Wongsiriroj
  • Mira Rosenblat
  • Andrew V Turnbull
  • Gene P Ables
  • Michael Aviram
  • Henry N Ginsberg
  • Xiaojing Shi
  • Claudio J Villanueva
  • Vinay Sachdev
  • Megan M Robblee
  • Maidina Tuohetahuntila
  • Yiying Zhang
  • Xiaoqing Mo
  • Joanna J Kaylor
  • Sylvaine Cases
  • Zoi Drosatos-Tampakaki
  • S Cases
  • Joseph L Dixon
  • Gerald Hoefler
  • Nancy Chen
  • Sascha Obrowsky
  • Dagmar Kratky
  • Feifei Yang
  • Hua V Lin
  • Aki Uchida
  • Kryscilla Jian Zhang Yang
  • Thomas O Eichmann
  • Linda K Buckett
  • Li Shin Huang
  • Ji Xin Cheng
  • Shuiqing Yu
  • Roseann Piantedosi
  • Prakash G Chandak
  • Haja R Wurie
  • Malin C Levin
  • Pamela J McFie
  • Kalyani G Bharadwaj
  • Zala Prevorsek
  • Edna Meilin
  • Bonggi Lee
  • Michelle Y S Shih
  • Mayumi Okawa
  • Steven J Smith
  • Sanjay Bhanot
  • Zuleika Ladha
  • Rodrigo M P Siloto
  • Kanji Yamaguchi
  • Sabina Paglialunga
  • Ying Liu
  • Simon Horvat
  • Takashi Hirata
  • Tomomi Yamazaki
  • Randall J Weselake
  • Andre Laroche
  • Jeffrey T Billheimer
  • R V Farese
  • S J Smith
  • Ioannis M Stylianou
  • Heather M Myers
  • Dagmar Kolb
  • Andreas Zimmer
  • Martijn R Molenaar
  • Meera K Shenoy
  • Jos F Brouwers
  • Martin Houweling
  • M Mahmood Hussain
  • J Bernd Helms

Detail Information

Publications74

  1. pmc Effects of DGAT1 deficiency on energy and glucose metabolism are independent of adiponectin
    Ryan S Streeper
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Am J Physiol Endocrinol Metab 291:E388-94. 2006
    Mice lacking acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), an enzyme that catalyzes the terminal step in triacylglycerol synthesis, have enhanced insulin sensitivity and are protected from obesity, a result of increased energy ..
  2. pmc Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
    Chi Liang Eric Yen
    Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53706, USA
    J Lipid Res 49:2283-301. 2008
    ..The genes encoding two DGAT enzymes, DGAT1 and DGAT2, were identified in the past decade, and the use of molecular tools, including mice deficient in either ..
  3. ncbi Role of adipocyte-derived factors in enhancing insulin signaling in skeletal muscle and white adipose tissue of mice lacking Acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    James A Haley Veterans Hospital, ACOS 151, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USA
    Diabetes 53:1445-51. 2004
    Mice that lack acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in mammalian triglyceride synthesis, have decreased adiposity and increased insulin sensitivity...
  4. ncbi Increased very low density lipoprotein secretion and gonadal fat mass in mice overexpressing liver DGAT1
    Tomomi Yamazaki
    Division of Clinical Nutrition, National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    J Biol Chem 280:21506-14. 2005
    ..The genes for two DGAT enzymes, DGAT1 and DGAT2, have been identified...
  5. ncbi The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94158, USA
    J Lipid Res 46:1502-11. 2005
    ..The two known DGATs, DGAT1 and DGAT2, are encoded by unrelated genes...
  6. ncbi Whole-body insulin resistance in the absence of obesity in FVB mice with overexpression of Dgat1 in adipose tissue
    Nancy Chen
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 West 168th St, PH 10 305J, New York, New York 10032, USA
    Diabetes 54:3379-86. 2005
    ..of triglyceride synthesis in adipose tissue by transgenic overexpression of the diacylglycerol aclytransferase-1 (Dgat1) gene causes obesity and/or alters insulin sensitivity...
  7. ncbi Short-term overexpression of DGAT1 or DGAT2 increases hepatic triglyceride but not VLDL triglyceride or apoB production
    John S Millar
    Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, USA
    J Lipid Res 47:2297-305. 2006
    ..We used adenoviral vectors containing either murine acyl-coenzyme A:diacylglycerol transferase 1 (DGAT1) or DGAT2 cDNA to determine the effect of a short-term increase in hepatic triglyceride synthesis on VLDL ..
  8. pmc Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance
    Li Liu
    Department of Medicine, Columbia University, New York, New York 10032, USA
    J Clin Invest 117:1679-89. 2007
    ..Transgenic overexpression of DGAT1 in mouse skeletal muscle replicated these findings and protected mice against high-fat diet-induced insulin ..
  9. doi Knockdown of acyl-CoA:diacylglycerol acyltransferase 2 with antisense oligonucleotide reduces VLDL TG and ApoB secretion in mice
    Ying Liu
    Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA 19104 6160, USA
    Biochim Biophys Acta 1781:97-104. 2008
    ..Similar results were obtained when DGAT1 KO mice were treated with the DGAT2 ASO...
  10. pmc The molecular basis of retinoid absorption: a genetic dissection
    Nuttaporn Wongsiriroj
    Institute of Human Nutrition and Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 283:13510-9. 2008
    ..Our present studies, employing a number of mutant mouse models, identify diacylglycerol acyltransferase 1 (DGAT1) as an important intestinal ARAT in vivo...
  11. ncbi Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat
    S J Smith
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California, USA
    Nat Genet 25:87-90. 2000
    ..Triglyceride synthesis has been assumed to occur primarily through acyl CoA:diacylglycerol transferase (Dgat), a microsomal enzyme that catalyses the final and only committed step in the glycerol phosphate pathway...
  12. pmc Retinol Esterification by DGAT1 Is Essential for Retinoid Homeostasis in Murine Skin
    Michelle Y S Shih
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    J Biol Chem 284:4292-9. 2009
    ..Here we show that the neutral lipid synthesis enzyme acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) functions as the major acyl-CoA:retinol acyltransferase (ARAT) in murine skin...
  13. pmc Specific role for acyl CoA:Diacylglycerol acyltransferase 1 (Dgat1) in hepatic steatosis due to exogenous fatty acids
    Claudio J Villanueva
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94158, USA
    Hepatology 50:434-42. 2009
    ..To investigate the role of acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), a key enzyme of TG synthesis, in fatty liver development, we studied mice with global and liver-specific ..
  14. pmc Paradoxical coupling of triglyceride synthesis and fatty acid oxidation in skeletal muscle overexpressing DGAT1
    Li Liu
    Department of Medicine, Preventive Medicine and Nutrition, Columbia University, New York, New York, USA
    Diabetes 58:2516-24. 2009
    Transgenic expression of diacylglycerol acyltransferase-1 (DGAT1) in skeletal muscle leads to protection against fat-induced insulin resistance despite accumulation of intramuscular triglyceride, a phenomenon similar to what is known as ..
  15. doi Role of MGAT2 and DGAT1 in the release of gut peptides after triglyceride ingestion
    Mayumi Okawa
    Tsukuba Research Institute, Banyu Pharmaceutical Company, Ltd, Tsukuba, Ibaraki 300 2611, Japan
    Biochem Biophys Res Commun 390:377-81. 2009
    ..STC-1 and GLUTag, GLP-1-producing intestinal endocrine L-cell lines, displayed a significant level of DGAT1 activity but not MGAT activity...
  16. pmc DGAT1-dependent triacylglycerol storage by macrophages protects mice from diet-induced insulin resistance and inflammation
    Suneil K Koliwad
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    J Clin Invest 120:756-67. 2010
    ..and to induce expression of the triacylglycerol (TG) synthesis enzyme acyl CoA: diacylglycerol acyltransferase 1 (DGAT1) in WAT and in adipocytes...
  17. pmc Intestine-specific expression of acyl CoA:diacylglycerol acyltransferase 1 reverses resistance to diet-induced hepatic steatosis and obesity in Dgat1-/- mice
    Bonggi Lee
    Department of Foods and Nutrition, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    J Lipid Res 51:1770-80. 2010
    Mice deficient in acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in triacylglycerol (TG) biosynthesis, are resistant to high-fat (HF) diet-induced hepatic steatosis and obesity...
  18. pmc Lack of acyl-CoA:diacylglycerol acyltransferase 1 reduces intestinal cholesterol absorption and attenuates atherosclerosis in apolipoprotein E knockout mice
    Prakash G Chandak
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, Harrachgasse 21, Graz, Austria
    Biochim Biophys Acta 1811:1011-20. 2011
    ..Lack of whole body DGAT1 is associated with reduced lipid-induced inflammation...
  19. pmc Targeting Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) with small molecule inhibitors for the treatment of metabolic diseases
    Jingsong Cao
    Biocorrection Research Unit, Pfizer, Cambridge, Massachusetts 02140, USA
    J Biol Chem 286:41838-51. 2011
    Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) is one of two known DGAT enzymes that catalyze the final step in triglyceride synthesis...
  20. pmc Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content
    Andreas Winter
    Lehrstuhl für Tierzucht der Technischen Universität München, 85350 Freising Weihenstephan, Germany
    Proc Natl Acad Sci U S A 99:9300-5. 2002
    b>DGAT1 encodes diacylglycerol O-acyltransferase (EC ), a microsomal enzyme that catalyzes the final step of triglyceride synthesis...
  21. ncbi Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members
    S Cases
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California 94143, USA
    J Biol Chem 276:38870-6. 2001
    ..DGAT2 is a member of a gene family that has no homology with DGAT1 and includes several mouse and human homologues that are candidates for additional DGAT genes...
  22. ncbi DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis
    Kimberly K Buhman
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Biol Chem 277:25474-9. 2002
    ..In this study, we analyzed intestinal triacylglycerol absorption and chylomicron synthesis and secretion in DGAT1-deficient (Dgat1(-/-)) mice...
  23. pmc Leptin modulates the effects of acyl CoA:diacylglycerol acyltransferase deficiency on murine fur and sebaceous glands
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Clin Invest 109:175-81. 2002
    ..Mice lacking DGAT1 (Dgat(-/-)) demonstrate significant changes in lipid metabolism in several tissues, including the skin...
  24. pmc Obesity resistance and enhanced glucose metabolism in mice transplanted with white adipose tissue lacking acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Clin Invest 111:1715-22. 2003
    ..Mice lacking acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in mammalian triglyceride synthesis, are protected against diet-induced obesity and glucose ..
  25. ncbi Lipopenia and skin barrier abnormalities in DGAT2-deficient mice
    Scot J Stone
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 1900, USA
    J Biol Chem 279:11767-76. 2004
    ..Gene knockout studies in mice have revealed that DGAT1 contributes to triglyceride synthesis in tissues and plays an important role in regulating energy metabolism but ..
  26. ncbi Posttranscriptional control of the expression and function of diacylglycerol acyltransferase-1 in mouse adipocytes
    Yi Hao Yu
    Department of Medicine, Institute of Human Nutrition, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 277:50876-84. 2002
    Acyl-CoA:diacylglycerol acyltransferase-1 (DGAT1) catalyzes the final step of triglyceride synthesis in mammalian cells. Data obtained from DGAT1-knockout mice have indicated that this enzyme plays an important role in energy homeostasis...
  27. ncbi Deficiency of acyl coenzyme a:diacylglycerol acyltransferase 1 increases leptin sensitivity in murine obesity models
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, University of California San Francisco, San Francisco, CA 94143, USA
    Endocrinology 143:2893-8. 2002
    Acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1) is one of two known enzymes that catalyze the final step in mammalian triglyceride synthesis...
  28. ncbi Analysis of energy expenditure at different ambient temperatures in mice lacking DGAT1
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94143, USA
    Am J Physiol Endocrinol Metab 284:E213-8. 2003
    Mice lacking acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in triglyceride synthesis, have increased energy expenditure and therefore are resistant to obesity...
  29. pmc Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis
    S Cases
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 95:13018-23. 1998
    ..Acyl CoA:diacylglycerol acyltransferase (DGAT, EC 2.3.1...
  30. pmc Increased insulin and leptin sensitivity in mice lacking acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, PO Box 419100, San Francisco, California 94141 9100, USA
    J Clin Invest 109:1049-55. 2002
    Acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1) is one of two known DGAT enzymes that catalyze the final step in mammalian triglyceride synthesis...
  31. pmc Development of the mammary gland requires DGAT1 expression in stromal and epithelial tissues
    Sylvaine Cases
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 1900, USA
    Development 131:3047-55. 2004
    ..We show that mice lacking the triglyceride synthesis enzyme acyl CoA:diacylglycerol transferase 1 (DGAT1) have impaired mammary gland development, characterized by decreased epithelial proliferation and alveolar ..
  32. doi Acyl-CoA:diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology
    Qin Liu
    Agricultural Lipid Biotechnology Program, Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6H 2P5
    Prog Lipid Res 51:350-77. 2012
    ..DGAT activity resides mainly in two distinct membrane bound polypeptides, known as DGAT1 and DGAT2 which have been identified in numerous organisms...
  33. pmc Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism
    Vinay Sachdev
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria
    Biochim Biophys Acta 1861:1132-41. 2016
    Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) is a key enzyme in triacylglycerol (TG) biosynthesis. Here we show that genetic deficiency and pharmacological inhibition of DGAT1 in mice alters cholesterol metabolism...
  34. pmc Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1
    Feifei Yang
    Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, P R China
    Lipids Health Dis 12:63. 2013
    The gene encoding diacylglycerol acyltransferase (DGAT1) is a functional and positional candidate gene for milk and intramuscular fat content...
  35. doi Congenic and bioinformatics analyses resolved a major-effect Fob3b QTL on mouse Chr 15 into two closely linked loci
    Zala Prevorsek
    Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230, Domzale, Slovenia
    Mamm Genome 21:172-85. 2010
    ..A high-resolution map along with prioritization of Fob3b1 candidates by bioinformatics represents an important step forward to final identification of the Chr 15 obesity QTL...
  36. pmc Intestinal acyl-CoA:diacylglycerol acyltransferase 2 overexpression enhances postprandial triglyceridemic response and exacerbates high fat diet-induced hepatic triacylglycerol storage
    Aki Uchida
    Purdue University, West Lafayette, IN, USA
    Biochim Biophys Acta 1831:1377-85. 2013
    ..mice with intestine-specific overexpression of DGAT2 and compared them with intestine-specific overexpression of DGAT1 and wild-type (WT) mice...
  37. pmc Loss of β-carotene 15,15'-oxygenase in developing mouse tissues alters esterification of retinol, cholesterol and diacylglycerols
    Joseph L Dixon
    Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA Rutgers Center for Lipid Research, Rutgers University, New Brunswick, NJ 08901, USA
    Biochim Biophys Acta 1841:34-43. 2014
    ....
  38. pmc Genetic dissection of retinoid esterification and accumulation in the liver and adipose tissue
    Nuttaporn Wongsiriroj
    Institute of Human Nutrition, Columbia University, New York, NY 10032
    J Lipid Res 55:104-14. 2014
    ..Our data establish that acyl-CoA:retinol acyltransferase (ARAT) activity is not involved in RE synthesis in the liver, even when mice are nutritionally stressed by feeding a 25-..
  39. pmc Palmitic acid and DGAT1 deficiency enhance osteoclastogenesis, while oleic acid-induced triglyceride formation prevents it
    Zoi Drosatos-Tampakaki
    Division of Periodontics, Columbia University College of Dental Medicine, New York, NY, USA
    J Bone Miner Res 29:1183-95. 2014
    ..Adenovirus-mediated expression of diacylglycerol acyl transferase 1 (DGAT1), a gene involved in triglyceride synthesis, also inhibits PA-induced osteoclastogenesis, suggesting a protective ..
  40. pmc F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression
    Xiaoqing Mo
    Department of Endocrinology, First Affiliated Hospital, Sun Yat Sen University, No 58 Zhongshan 2nd road, Guangzhou 510080, PR China
    PLoS ONE 10:e0117156. 2015
    ..In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice...
  41. pmc ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells
    Maidina Tuohetahuntila
    Departments of Biochemistry and Cell Biology Faculty of Veterinary Medicine and Institute of Biomembranes, Utrecht University, 3584 CM Utrecht, The Netherlands
    J Lipid Res 57:1162-74. 2016
    ..Compared with mouse HSCs, rat HSCs have a higher turnover of new TAGs, and Atglistatin and the DGAT1 inhibitor, T863, were more effective...
  42. pmc Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium
    Joanna J Kaylor
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 10:e0125921. 2015
    ..Here we show that diacylglycerol O-acyltransferase-1 (DGAT1) is expressed in multiple cells of the retina including RPE and Müller glial cells...
  43. pmc Saturated Fatty Acids Engage an IRE1α-Dependent Pathway to Activate the NLRP3 Inflammasome in Myeloid Cells
    Megan M Robblee
    Diabetes Center, University of California San Francisco, San Francisco, CA 94143, USA Biomedical Sciences Graduate Program, University of California San Francisco, San Francisco, CA 94143, USA
    Cell Rep 14:2611-23. 2016
    ..Thus, IRE1α is an unrecognized intracellular PC sensor critical to the process by which SFAs stimulate macrophages to secrete IL-1β, a driver of diet-induced tissue inflammation. ..
  44. pmc Studies on the substrate and stereo/regioselectivity of adipose triglyceride lipase, hormone-sensitive lipase, and diacylglycerol-O-acyltransferases
    Thomas O Eichmann
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    J Biol Chem 287:41446-57. 2012
    ..Because ATGL preferentially generates sn-1,3 and sn-2,3, it suggests that TAG-derived DAG cannot directly enter phospholipid synthesis or activate protein kinase C without prior isomerization...
  45. pmc Intestinal DGAT1 deficiency reduces postprandial triglyceride and retinyl ester excursions by inhibiting chylomicron secretion and delaying gastric emptying
    Gene P Ables
    Department of Medicine, Columbia University, New York, NY, USA
    J Lipid Res 53:2364-79. 2012
    ..We show that acute administration of a DGAT1 inhibitor (DGAT1i) by oral gavage or genetic deletion of intestinal Dgat1 (intestine-Dgat1(-/-)) markedly reduced ..
  46. pmc Deficiency of the lipid synthesis enzyme, DGAT1, extends longevity in mice
    Ryan S Streeper
    Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA
    Aging (Albany NY) 4:13-27. 2012
    ..We show here that deficiency of the triglyceride synthesis enzyme acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), which promotes leanness, also extends longevity without limiting food intake...
  47. pmc Hepatitis C virus core protein decreases lipid droplet turnover: a mechanism for core-induced steatosis
    Charles Harris
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California 94158, USA
    J Biol Chem 286:42615-25. 2011
    ..lipid droplets (LDs) in infected liver cells through a process dependent on host diacylglycerol acyltransferase 1 (DGAT1), an enzyme that synthesizes triglycerides in the endoplasmic reticulum...
  48. pmc Diacylglycerol acyltransferase-1 (DGAT1) inhibition perturbs postprandial gut hormone release
    Hua V Lin
    Merck Research Laboratories, Rahway, New Jersey, USA
    PLoS ONE 8:e54480. 2013
    Diacylglycerol acyltransferase-1 (DGAT1) is a potential therapeutic target for treatment of obesity and related metabolic diseases. However, the degree of DGAT1 inhibition required for metabolic benefits is unclear...
  49. ncbi Mammalian acyl-CoA:cholesterol acyltransferases
    K F Buhman
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 9100, USA
    Biochim Biophys Acta 1529:142-54. 2000
    ..These insights may have relevance for the development of ACAT inhibitors for treating hypercholesterolemia or atherosclerosis in humans...
  50. pmc DGAT1 expression increases heart triglyceride content but ameliorates lipotoxicity
    Li Liu
    Department of Medicine, Division of Preventive Medicine and Metabolism, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 284:36312-23. 2009
    ..greater TG stores and a marked induction of the TG-synthesizing enzyme diacylglycerol (DAG) acyltransferase 1 (DGAT1)...
  51. pmc Evidence that diacylglycerol acyltransferase 1 (DGAT1) has dual membrane topology in the endoplasmic reticulum of HepG2 cells
    Haja R Wurie
    Metabolic and Vascular Health, Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Biol Chem 286:36238-47. 2011
    ..A. (1997) Biochem. J. 323, 17-21). Recent topographical studies suggested that the oligomeric enzyme DGAT1 is exclusively lumen facing (latent) in the ER membrane...
  52. pmc DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes
    Charles A Harris
    Gladstone Institute for Cardiovascular Disease, Department of Medicine, University of California, San Francisco, CA 94158, USA
    J Lipid Res 52:657-67. 2011
    The total contribution of the acyl CoA:diacylglycerol acyltransferase (DGAT) enzymes, DGAT1 and DGAT2, to mammalian triacylglycerol (TG) synthesis has not been determined...
  53. pmc A murine model of isolated cardiac steatosis leads to cardiomyopathy
    DENIS J GLENN
    Department of Medicine and Diabetes Center, University of California at San Francisco, San Francisco, CA 94143 0540, USA
    Hypertension 57:216-22. 2011
    ..We hypothesized that forced expression of DGAT1 in the cardiac myocyte would result in increased lipid accumulation and heart dysfunction...
  54. doi An N-terminal fragment of mouse DGAT1 binds different acyl-CoAs with varying affinity
    Rodrigo M P Siloto
    Department of Agricultural, Food and Nutritional Science, 4 10 Agriculture Forestry Centre, University of Alberta, Edmonton, Alta, Canada T6G 2P5
    Biochem Biophys Res Commun 373:350-4. 2008
    ..assays indicated that MmDGAT1(1-95)His(6) interacted with long chain fatty acyl-CoAs similar to observations on DGAT1 from oilseed rape (Brassica napus)...
  55. ncbi Novel QTLs for HDL levels identified in mice by controlling for Apoa2 allelic effects: confirmation of a chromosome 6 locus in a congenic strain
    Carrie L Welch
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    Physiol Genomics 17:48-59. 2004
    ..003 and P = 0.0001 for HDL-C and non-HDL-C levels, respectively). These data are consistent with polygenic inheritance of HDL-C levels in the mouse model and provide candidate loci for HDL-C and non-HDL-C level determination in humans...
  56. ncbi Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts
    Carolina Bagnato
    Instituto de Investigaciones Bioquímicas de La Plata INIBIOLP, CONICET UNLP, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, calles 60 y 120, 1900 La Plata, Argentina
    J Biol Chem 278:52203-11. 2003
    ..To test this hypothesis, we stably overexpressed the DGAT1 mouse gene in human lung SV40-transformed fibroblasts (DGAT cells), which contains high levels of DAG...
  57. ncbi Genetic complexity of an obesity QTL ( Fob3) revealed by detailed genetic mapping
    Ioannis M Stylianou
    Roslin Institute Edinburgh, Roslin, EH25 9PS, Scotland, UK
    Mamm Genome 15:472-81. 2004
    ....
  58. ncbi Concerted elevation of acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin
    Rupalie L Meegalla
    Department of Central Nervous System Diseases, Bristol Myers Squibb Company, Experimental Station, E400 5269, Rt 141 and Henry Clay Road, Wilmington, DE 19880, USA
    Biochem Biophys Res Commun 298:317-23. 2002
    ..In the current study, we report that glucose preferentially enhances DGAT1 mRNA expression, whereas insulin specifically increases the level of DGAT2 mRNA...
  59. pmc The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment
    Gouri Ranganathan
    Central Arkansas Veterans Healthcare System, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Lipid Res 47:2444-50. 2006
    ..To study the relationship of DGAT1, FAS, and LPL with insulin, we examined adipose mRNA expression of these genes in subjects with a wide range of ..
  60. ncbi Activation of diacylglycerol O-acyltransferase 1 gene results in increased tumor necrosis factor-alpha gene expression in 3T3-L1 adipocytes
    Takashi Hirata
    Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260 8675, Japan
    FEBS Lett 580:5117-21. 2006
    ..The expression of TNF-alpha, DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25 mM glucose...
  61. pmc Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet
    Steven J Y Wang
    Clore Laboratory, University of Buckingham, Buckingham MK18 1EG, UK
    Lipids Health Dis 6:2. 2007
    Mice that lack acyl CoA:diacylglycerol acyltransferase (Dgat1-/- mice) are reported to have a reduced body fat content and improved glucose tolerance and insulin sensitivity...
  62. ncbi Diacylglycerol acyltranferase 1 anti-sense oligonucleotides reduce hepatic fibrosis in mice with nonalcoholic steatohepatitis
    Kanji Yamaguchi
    Division of Gastroenterology, Duke University Medical Center, Durham, NC, USA
    Hepatology 47:625-35. 2008
    ..reports, LRAT(-/-) mice had reduced hepatic RE but neither excessive HSC activation nor liver fibrosis, and DGAT1(-/-) mice had increased liver levels of RE and retinol. We sought to clarify the role of DGAT1 in liver fibrosis...
  63. ncbi Acylation-stimulating protein deficiency and altered adipose tissue in alternative complement pathway knockout mice
    Sabina Paglialunga
    Department of Biochemistry, McGill University, Montreal
    Am J Physiol Endocrinol Metab 294:E521-9. 2008
    ..was increased by 85% in obese ob/ob leptin-deficient mice and decreased in lean diacylglycerol acyltransferase 1 (DGAT1) KO mice (-54%) and C/EBPalpha(beta/beta) transgenic mice (-70%) compared with WT...
  64. pmc Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 1900, USA
    Proc Natl Acad Sci U S A 99:8512-7. 2002
    ..The identification of the MGAT1 gene should greatly facilitate research on the identification of the intestinal MGAT gene and on the function of MGAT enzymes in mammalian glycerolipid metabolism...
  65. doi Pomegranate juice protects macrophages from triglyceride accumulation: inhibitory effect on DGAT1 activity and on triglyceride biosynthesis
    Mira Rosenblat
    The Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences, Rambam Medical Center, Haifa, Israel
    Ann Nutr Metab 58:1-9. 2011
    ..To analyze the effects of pomegranate juice (PJ) and punicalagin on macrophage triglyceride metabolism...
  66. ncbi Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice
    I J Waterman
    Cell Biochemistry, Hannah Research Institute, Ayr, Scotland, UK
    Int J Obes Relat Metab Disord 26:742-3. 2002
    ..Overt and latent hepatic DGAT activities for 16-week-old lean Ob/? mice were 0.42+/-0.14 and 1.57+/-0.04 micromol/mg/min, respectively...
  67. pmc Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption
    Dong Cheng
    Department of Metabolic Diseases, Bristol Myers Squibb Company, Princeton, New Jersey 08543 5400, USA
    J Biol Chem 283:29802-11. 2008
    Acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1) is one of the four intestinal membrane bound acyltransferases implicated in dietary fat absorption...
  68. pmc The endoplasmic reticulum enzyme DGAT2 is found in mitochondria-associated membranes and has a mitochondrial targeting signal that promotes its association with mitochondria
    Scot J Stone
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    J Biol Chem 284:5352-61. 2009
    ..Thus, DGAT2, an ER-resident transmembrane domain-containing enzyme, is also found in mitochondria-associated membranes, where its N terminus may promote its association with mitochondria...
  69. pmc Paraoxonase 2 attenuates macrophage triglyceride accumulation via inhibition of diacylglycerol acyltransferase 1
    Mira Rosenblat
    Lipid Research Laboratory, Technion Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, Rambam Medical Center, Haifa, Israel
    J Lipid Res 50:870-9. 2009
    ..but not cholesterol) content, biosynthesis rate, and microsomal acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) activity (not mRNA and protein) in PON2-deficient versus control MPM were all significantly increased by 4.6-, 3...
  70. ncbi Characterization of cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica napus and sucrose-mediated induction of enzyme biosynthesis
    Cory L Nykiforuk
    Department of Chemistry and Biochemistry, University of Lethbridge, 4401 University Drive, T1K 3M4, Lethbridge, AB, Canada
    Biochim Biophys Acta 1580:95-109. 2002
    cDNAs encoding acyl-CoA:diacylglycerol acyltransferase (DGAT, EC 2.3.1.20), designated BnDGAT1 and BnDGAT2, were obtained from a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv Jet Neuf)...
  71. ncbi Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition
    Bernard Grisart
    Department of Genetics, Faculty of Veterinary Medicine, University of Liège B43, 4000 Liege, Belgium
    Genome Res 12:222-31. 2002
    ..marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a ..
  72. doi Paraoxonase 2 (PON2) decreases high glucose-induced macrophage triglycerides (TG) accumulation, via inhibition of NADPH-oxidase and DGAT1 activity: studies in PON2-deficient mice
    Edna Meilin
    The Lipid Research Laboratory, Technion, The Rappaport Family Institute for Research in the Medical Science, Rambam Medical Center, Haifa, Israel
    Atherosclerosis 208:390-5. 2010
    ..The present study investigates the role of paraoxonase 2 (PON2) in the attenuation of macrophage triglycerides (TG) biosynthesis, and oxidative stress, under diabetic conditions...
  73. pmc Topological orientation of acyl-CoA:diacylglycerol acyltransferase-1 (DGAT1) and identification of a putative active site histidine and the role of the n terminus in dimer/tetramer formation
    Pamela J McFie
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    J Biol Chem 285:37377-87. 2010
    ..Two DGAT enzymes have been identified (DGAT1 and DGAT2) with unique roles in lipid metabolism...
  74. pmc DGAT1 deficiency decreases PPAR expression and does not lead to lipotoxicity in cardiac and skeletal muscle
    Li Liu
    Division of Preventive Medicine and Nutrition, Columbia University, New York, NY, USA
    J Lipid Res 52:732-44. 2011
    Diacylglycerol (DAG) acyl transferase 1 (Dgat1) knockout ((-/-)) mice are resistant to high-fat-induced obesity and insulin resistance, but the reasons are unclear...

Research Grants2

  1. DGAT Overexpression In Mouse Adipose Tissue and Muscle
    Yi Hao Yu; Fiscal Year: 2006
    ..Two transgenic mouse models with tissue-specific overexpression of diacylglycerol acyl transferase (DGAT) will be studied. DGAT is a key enzyme in triglyceride (TG) synthesis...
  2. The Role of DGAT1 In Muscle Lipotoxicity
    Yi Hao Yu; Fiscal Year: 2006
    ..the development of steatosis, we propose to develop two transgenic mice with tissue-specific overexpression of the Dgat1 (diacylglycerol acyltransferase-1, or DGAT1) gene...