Cryaa

Summary

Gene Symbol: Cryaa
Description: crystallin, alpha A
Alias: Acry-1, Crya-1, Crya1, DAcry-1, lop18, alpha-crystallin A chain, crystallin, alpha 1, lens opacity 18
Species: mouse

Top Publications

  1. ncbi Mapping of the Pim-1 oncogene in mouse t-haplotypes and its use to define the relative map positions of the tcl loci t0(t6) and tw12 and the marker tf (tufted)
    B Ark
    Department of Zoology, University of Texas, Austin 78712 1064
    Genomics 10:385-9. 1991
  2. ncbi Ly-39: a new lymphocyte alloantigen defined with a monoclonal antibody
    E Park
    Immunology Branch, National Cancer Institute, Bethesda, MD 20892
    Immunogenetics 28:64-6. 1988
  3. pmc Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia
    A B Chepelinsky
    Proc Natl Acad Sci U S A 82:2334-8. 1985
  4. ncbi Differential expression of alpha A- and alpha B-crystallin during murine ocular development
    M L Robinson
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 37:2276-84. 1996
  5. pmc Altered chaperone-like activity of alpha-crystallins promotes cataractogenesis
    Catherine Cheng
    School of Optometry and Vision Science Program, University of California, Berkeley, California 94720, USA
    J Biol Chem 285:41187-93. 2010
  6. pmc A forkhead gene, FoxE3, is essential for lens epithelial proliferation and closure of the lens vesicle
    A Blixt
    Department of Molecular Biology, Goteborg University, The Lundberg Laboratory, Medicinaregatan 9C, Box 462, S 405 30 Goteborg, Sweden
    Genes Dev 14:245-54. 2000
  7. ncbi Characterization of a new, dominant V124E mutation in the mouse alphaA-crystallin-encoding gene
    J Graw
    National Research Center for Environment and Health GSF, Institute of Mammalian Genetics, Neuherberg, Germany
    Invest Ophthalmol Vis Sci 42:2909-15. 2001
  8. pmc HSF4 is required for normal cell growth and differentiation during mouse lens development
    Mitsuaki Fujimoto
    Department of Biochemistry and Molecular Biology, Yamaguchi University School of Medicine, Minami Kogushi 1 1 1, Ube 755 8505, Japan
    EMBO J 23:4297-306. 2004
  9. pmc A transgenic mouse model for human autosomal dominant cataract
    Cheng Da Hsu
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri, USA
    Invest Ophthalmol Vis Sci 47:2036-44. 2006
  10. doi Elevated retina-specific expression of the small heat shock protein, alphaA-crystallin, is associated with photoreceptor protection in experimental uveitis
    Narsing A Rao
    Doheny Eye Institute, Keck School of Mewdicine, University of Southern California, Los Angeles, CA 90033, USA
    Invest Ophthalmol Vis Sci 49:1161-71. 2008

Research Grants

Scientific Experts

  • N A Rao
  • Haotian Zhao
  • Usha Andley
  • J Graw
  • Priyanka Arya
  • C Slingsby
  • A Cvekl
  • Olga Medina-Martinez
  • Louise Wolf
  • David Maerker
  • A Menko
  • Viktor Morozov
  • Ram Kannan
  • Muriel Davisson
  • Mark A Erhart
  • B Chang
  • Daniel L Boyle
  • Jing Hua Xi
  • Ying Yang
  • Ohad Shaham
  • Fang Bai
  • Catherine Cheng
  • Qingling Huang
  • Chun hong Xia
  • Jennifer Yaung
  • Qing Xie
  • Ruth Ashery-Padan
  • Ying Liu
  • Michael L Robinson
  • Wei Lin Wang
  • Ramya Rajagopal
  • Jie Huang
  • David C Beebe
  • Joseph Horwitz
  • Xiaohua Gong
  • Ryuichi Yamada
  • Cheng Da Hsu
  • Eric F Wawrousek
  • Rooban B Nahomi
  • Dinesh Upadhya
  • W F Hu
  • Christian Carbe
  • Ryan C Widau
  • Ram H Nagaraj
  • Benlian Wang
  • Claudia M Garcia
  • Doreswamy Kenchegowda
  • Pamela L Wenzel
  • Mandy K Losiewicz
  • Shengwei Ren
  • Ondrej Machon
  • Matthew A Reilly
  • Mahesha H Gangadhariah
  • Shuying He
  • Linlin Ding
  • Bin Zhang
  • Takashi Takeuchi
  • Kelly A Barton
  • Xin Zhang
  • April N Smith
  • Richard A Lang
  • Kveta Cveklova
  • Bharesh K Chauhan
  • Yan Zhang
  • Eric C Swindell
  • Yu V Rumyantseva
  • David R Hinton
  • Lixing W Reneker
  • J Mark Petrash
  • Christine Spee
  • Akira Nakai
  • Asa Blixt
  • Ossama B Kashlan
  • Yi Pan
  • Aki Yoshimoto
  • Mitsuaki Fujimoto
  • Yoko Mizutani-Koseki
  • Naoki Takahashi
  • Haruhiko Koseki
  • Puwat Charukamnoetkanok
  • S H Pilder
  • Mariko Hirano
  • Daryle J DePianto
  • Neena B Haider
  • Guy Goudreau
  • Jana Löster
  • Seung Whan Kim
  • Aileen Sandilands
  • Karen Gueta
  • Pazit Oren-Giladi

Detail Information

Publications111 found, 100 shown here

  1. ncbi Mapping of the Pim-1 oncogene in mouse t-haplotypes and its use to define the relative map positions of the tcl loci t0(t6) and tw12 and the marker tf (tufted)
    B Ark
    Department of Zoology, University of Texas, Austin 78712 1064
    Genomics 10:385-9. 1991
    ..The mapping of two allelic t-lethals, t0 and t6 with respect to tw12 and tf has also been a problem.(ABSTRACT TRUNCATED AT 250 WORDS)..
  2. ncbi Ly-39: a new lymphocyte alloantigen defined with a monoclonal antibody
    E Park
    Immunology Branch, National Cancer Institute, Bethesda, MD 20892
    Immunogenetics 28:64-6. 1988
  3. pmc Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia
    A B Chepelinsky
    Proc Natl Acad Sci U S A 82:2334-8. 1985
    ..These experiments also suggest that explanted epithelia from other tissues may be useful for studying the expression of foreign genes...
  4. ncbi Differential expression of alpha A- and alpha B-crystallin during murine ocular development
    M L Robinson
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 37:2276-84. 1996
    ..To compare the temporal and spatial expression patterns of alpha A- and alpha B-crystallin mRNA during ocular development...
  5. pmc Altered chaperone-like activity of alpha-crystallins promotes cataractogenesis
    Catherine Cheng
    School of Optometry and Vision Science Program, University of California, Berkeley, California 94720, USA
    J Biol Chem 285:41187-93. 2010
    ..Crystallins with different chaperone-like activities are likely required in the lens cortex and nucleus for maintaining transparency...
  6. pmc A forkhead gene, FoxE3, is essential for lens epithelial proliferation and closure of the lens vesicle
    A Blixt
    Department of Molecular Biology, Goteborg University, The Lundberg Laboratory, Medicinaregatan 9C, Box 462, S 405 30 Goteborg, Sweden
    Genes Dev 14:245-54. 2000
    ..This implies that FoxE3 is essential for closure of the lens vesicle and is a factor that promotes survival and proliferation, while preventing differentiation, in the lens epithelium...
  7. ncbi Characterization of a new, dominant V124E mutation in the mouse alphaA-crystallin-encoding gene
    J Graw
    National Research Center for Environment and Health GSF, Institute of Mammalian Genetics, Neuherberg, Germany
    Invest Ophthalmol Vis Sci 42:2909-15. 2001
    ..The purpose of the study was morphologic description, mapping of the mutant gene, and characterization of the underlying molecular lesion in a particular mutant, Aey7...
  8. pmc HSF4 is required for normal cell growth and differentiation during mouse lens development
    Mitsuaki Fujimoto
    Department of Biochemistry and Molecular Biology, Yamaguchi University School of Medicine, Minami Kogushi 1 1 1, Ube 755 8505, Japan
    EMBO J 23:4297-306. 2004
    ....
  9. pmc A transgenic mouse model for human autosomal dominant cataract
    Cheng Da Hsu
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri, USA
    Invest Ophthalmol Vis Sci 47:2036-44. 2006
    ..To characterize lenses from transgenic mice designed to express mutant and wild-type alphaA-crystallin subunits...
  10. doi Elevated retina-specific expression of the small heat shock protein, alphaA-crystallin, is associated with photoreceptor protection in experimental uveitis
    Narsing A Rao
    Doheny Eye Institute, Keck School of Mewdicine, University of Southern California, Los Angeles, CA 90033, USA
    Invest Ophthalmol Vis Sci 49:1161-71. 2008
    ..However, no apoptosis has been detected during this phase. In this study, alphaA-crystallin upregulation in the retina and its antiapoptotic protective role were evaluated in early EAU...
  11. pmc Mechanism of cataract formation in alphaA-crystallin Y118D mutation
    Qingling Huang
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California 90095, USA
    Invest Ophthalmol Vis Sci 50:2919-26. 2009
    ..The aim of this study was to elucidate the molecular mechanisms that lead to a dominant nuclear cataract in a mouse harboring the Y118D mutation in the alphaA-crystallin gene...
  12. pmc The orchestration of mammalian tissue morphogenesis through a series of coherent feed-forward loops
    Qing Xie
    Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 286:43259-71. 2011
    ....
  13. ncbi αA- and αB-crystallins interact with caspase-3 and Bax to guard mouse lens development
    W F Hu
    Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
    Curr Mol Med 12:177-87. 2012
    ..Together, our results further confirm that αA and αB regulate caspase-3 and Bax in vitro and in vivo to regulate lens differentiation...
  14. ncbi p53-dependent apoptosis produced by Rb-deficiency in the developing mouse lens
    S D Morgenbesser
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461
    Nature 371:72-4. 1994
    ..This developmental system provides a framework for understanding the consequences of the frequent mutation of both RB and p53 in human cancer...
  15. pmc Targeted disruption of the mouse alpha A-crystallin gene induces cataract and cytoplasmic inclusion bodies containing the small heat shock protein alpha B-crystallin
    J P Brady
    National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
    Proc Natl Acad Sci U S A 94:884-9. 1997
    ..These studies suggest that alpha A is essential for maintaining lens transparency, possibly by ensuring that alpha B or proteins closely associated with this small heat shock protein remain soluble...
  16. ncbi The molecular chaperone alphaA-crystallin enhances lens epithelial cell growth and resistance to UVA stress
    U P Andley
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:31252-61. 1998
    ..These results indicate that alphaA has important cellular functions in the lens over and above its well characterized role in refraction...
  17. ncbi Prox1 function is crucial for mouse lens-fibre elongation
    J T Wigle
    Department of Genetics, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nat Genet 21:318-22. 1999
    ..Our data provide evidence that the progression of terminal fibre differentiation and elongation is dependent on Prox1 activity during lens development...
  18. ncbi Regulation of lens fiber cell differentiation by transcription factor c-Maf
    S Kawauchi
    Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
    J Biol Chem 274:19254-60. 1999
    ..The expression of crystallin genes was severely impaired in the c-maf-null mutant mouse lens. These results demonstrate that c-Maf is an indispensable regulator of lens differentiation during murine development...
  19. ncbi Regulation of mouse lens fiber cell development and differentiation by the Maf gene
    B Z Ring
    Department of Pediatrics, Howard Hughes Medical Institute, Stanford, California 94305 5428, USA
    Development 127:307-17. 2000
    ..Our results indicate that Maf directly activates many if not all of the (beta)-crystallin genes, and suggest a model for coordinating cell cycle withdrawal with terminal differentiation...
  20. ncbi Microphthalmia due to p53-mediated apoptosis of anterior lens epithelial cells in mice lacking the CREB-2 transcription factor
    T Hettmann
    The Laboratory of Cardiovascular Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Dev Biol 222:110-23. 2000
    ..Taken together, these results identify CREB-2 as an important regulator of mammalian lens development...
  21. pmc Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye
    R Ashery-Padan
    Max Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Goettingen D 37077, Germany
    Genes Dev 14:2701-11. 2000
    ....
  22. ncbi Regulation of GSH in alphaA-expressing human lens epithelial cell lines and in alphaA knockout mouse lenses
    R Kannan
    Division of Gastrointestinal and Liver Diseases, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
    Invest Ophthalmol Vis Sci 42:409-16. 2001
    ..To study the mechanism of regulation of GSH in HLE-B3 cells expressing alphaA-crystallin (alphaA) and in alphaA knockout mouse lenses...
  23. pmc Altered aggregation properties of mutant gamma-crystallins cause inherited cataract
    Aileen Sandilands
    Department of Biochemistry, Medical Science Institutes, University of Dundee, Dundee DD1 5EH, Scotland, UK
    EMBO J 21:6005-14. 2002
    ..The mutant gamma-crystallins initially disrupt nuclear function, but then this progresses to a full cataract phenotype...
  24. pmc Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice: implication for posterior lenticonus with cataract
    Seung Whan Kim
    Department of Life Science, Pohang University of Science and Technology, Pohang 790 784, Korea
    Mol Cell Biol 22:8409-14. 2002
    ..Our results provide a novel insight into the molecular and histopathological mechanism of posterior lenticonus with cataract and attest to the importance of ASC-2 as a pivotal transcriptional coactivator of nuclear receptors in vivo...
  25. ncbi Reduced survival of lens epithelial cells in the alphaA-crystallin-knockout mouse
    Jing Hua Xi
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Sci 116:1073-85. 2003
    ..These data suggest that alphaA expression in vivo protects against cell death during mitosis in the lens epithelium, and the smaller size of the alphaA(-/-) lens may be due to a decrease in the net production of epithelial cells...
  26. ncbi Cell-autonomous involvement of Mab21l1 is essential for lens placode development
    Ryuichi Yamada
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Development 130:1759-70. 2003
    ..We conclude that Mab21l1 expression dependent on PAX6 is essential for lens placode growth and for formation of the lens vesicle; lack of Mab21l1 expression causes reduced expression of Foxe3 in a cell-autonomous manner...
  27. pmc Tissue-specific regulation of the mouse alphaA-crystallin gene in lens via recruitment of Pax6 and c-Maf to its promoter
    Ying Yang
    The Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Mol Biol 351:453-69. 2005
    ..In addition, the data suggest a molecular model of temporal and spatial regulation of alphaB, alphaA and gamma-crystallin genes in mouse embryonic lens by using variants of the Pax6/Maf regulatory module...
  28. ncbi Regulation of ocular lens development by Smad-interacting protein 1 involving Foxe3 activation
    Aki Yoshimoto
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Development 132:4437-48. 2005
    ..26 kb promoter, and are separate from lens-specific regulation. This is the first demonstration of the significance of Smad interaction in modulating Sip1 activity...
  29. ncbi Caspase-dependent secondary lens fiber cell disintegration in alphaA-/alphaB-crystallin double-knockout mice
    Viktor Morozov
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Building 7, 7 Memorial Drive, MSC 0704, Bethesda, MD 20892, USA
    Development 133:813-21. 2006
    ....
  30. pmc Regulation of alphaA-crystallin via Pax6, c-Maf, CREB and a broad domain of lens-specific chromatin
    Ying Yang
    Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    EMBO J 25:2107-18. 2006
    ..Our data demonstrate a novel mechanism of Pax6, c-Maf and CREB function, through regulation of chromatin-remodeling enzymes, and suggest a multistage model for the activation of alphaA-crystallin during lens differentiation...
  31. pmc alpha-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress
    Jennifer Yaung
    Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Mol Vis 13:566-77. 2007
    ..To investigate the susceptibility of retinal pigment epithelium (RPE) from alphaA (-/-) and alphaB (-/-) mice to oxidative stress, and the subcellular changes of alphaA and alphaB-crystallins under oxidative stress...
  32. pmc Distinct embryonic expression and localization of CBP and p300 histone acetyltransferases at the mouse alphaA-crystallin locus in lens
    Ying Yang
    Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY10461, USA
    J Mol Biol 369:917-26. 2007
    ..However, the lens-specific chromatin domain contains both promoter localized CBP on the "background" of locus-spread presence of CBP and p300...
  33. ncbi Up-regulation of tau, a brain microtubule-associated protein, in lens cortical fractions of aged alphaA-, alphaB-, and alphaA/B-crystallin knockout mice
    Fang Bai
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Vis 13:1589-600. 2007
    ..In the present study we examined the expression of the brain microtubule associated protein tau in lenses of alpha-crystallin gene knockout mice...
  34. pmc Mechanism of insolubilization by a single-point mutation in alphaA-crystallin linked with hereditary human cataracts
    Usha P Andley
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 47:9697-706. 2008
    ..Our study further shows that analysis of mutant proteins from the mouse model is an effective way to understand the mechanism of protein insolubilization in hereditary cataracts...
  35. pmc Interactions between small heat shock protein alpha-crystallin and galectin-related interfiber protein (GRIFIN) in the ocular lens
    Kelly A Barton
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 48:3956-66. 2009
    ..The binding interaction between alpha-crystallin and GRIFIN is enhanced up to 5-fold in the presence of 3 mM ATP. These binding data support the hypothesis that GRIFIN is a novel binding partner of alpha-crystallin in the lens...
  36. pmc Pax6 is essential for lens fiber cell differentiation
    Ohad Shaham
    Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
    Development 136:2567-78. 2009
    ..This study reveals that Pax6 is pivotal for initiation of the lens fiber differentiation program in the mammalian eye...
  37. pmc AlphaA-crystallin R49Cneo mutation influences the architecture of lens fiber cell membranes and causes posterior and nuclear cataracts in mice
    Usha P Andley
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri, USA
    BMC Ophthalmol 9:4. 2009
    AlphaA-crystallin (CRYAA/HSPB4), a major component of all vertebrate eye lenses, is a small heat shock protein responsible for maintaining lens transparency...
  38. doi Lens morphogenesis is dependent on Pax6-mediated inhibition of the canonical Wnt/beta-catenin signaling in the lens surface ectoderm
    Ondrej Machon
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 14420 Prague 4, Czech Republic
    Genesis 48:86-95. 2010
    ..Thus Pax6 is required for down-regulation of canonical Wnt signaling in the presumptive lens ectoderm...
  39. pmc In vivo lens deficiency of the R49C alphaA-crystallin mutant
    Usha P Andley
    Ophthalmology and Visual Sciences, Washington University in St Louis School of Medicine, 660 S Euclid Ave, Box 8096, St Louis, MO 63110, USA
    Exp Eye Res 90:699-702. 2010
    ..The death of fiber cells caused by this mutation may ultimately lead to loss of retinal integrity and blindness...
  40. pmc Lens fiber cell differentiation and denucleation are disrupted through expression of the N-terminal nuclear receptor box of NCOA6 and result in p53-dependent and p53-independent apoptosis
    Wei Lin Wang
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biol Cell 21:2453-68. 2010
    ..Our data demonstrate a cell-autonomous role of Ncoa6 in lens fiber cell differentiation and suggest novel insights into the process of lens fiber cell denucleation and apoptosis...
  41. doi Inactivation of Smad4 leads to impaired ocular development and cataract formation
    Ying Liu
    Department of Ophthalmology and Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Biochem Biophys Res Commun 400:476-82. 2010
    ..However, the functional role of Smad4 in controlling different visual system compartments has not been fully investigated...
  42. pmc Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and its denucleation
    Shuying He
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Epigenetics Chromatin 3:21. 2010
    ..Brg1 also controls neural stem cell maintenance, terminal differentiation of multiple cell lineages and organs including the T-cells, glial cells and limbs...
  43. pmc The function of FGF signaling in the lens placode
    Claudia M Garcia
    Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63110, USA
    Dev Biol 351:176-85. 2011
    ..Since the expression of proteins required for lens formation was not altered in the knockout placode cells, we can conclude that FGF signaling from the optic vesicle is not required for lens induction...
  44. pmc Pax6 regulates gene expression in the vertebrate lens through miR-204
    Ohad Shaham
    Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
    PLoS Genet 9:e1003357. 2013
    ..Collectively, this study identifies a novel evolutionarily conserved mechanism by which Pax6 controls the down-regulation of multiple genes through direct up-regulation of miR-204...
  45. pmc Comparative proteomic analysis identifies age-dependent increases in the abundance of specific proteins after deletion of the small heat shock proteins αA- and αB-crystallin
    Usha P Andley
    Department of Ophthalmology and Visual Sciences, Washington University, School of Medicine, St Louis, MO 63110, USA
    Biochemistry 52:2933-48. 2013
    ....
  46. ncbi Alpha-crystallin expression affects microtubule assembly and prevents their aggregation
    Jing Hua Xi
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, 660 S Euclid Ave, Campus Box 8096, St Louis, Missouri 63110, USA
    FASEB J 20:846-57. 2006
    ..SDS-PAGE analysis showed that alpha-crystallin prevented heat-induced aggregation of tubulin, suggesting that alpha-crystallin may affect microtubule assembly by maintaining the pool of unassembled tubulin...
  47. ncbi Arginine 54 and Tyrosine 118 residues of {alpha}A-crystallin are crucial for lens formation and transparency
    Chun hong Xia
    School of Optometry and Vision Science Program, University of California, Berkeley, Berkeley, California 94720 2020, USA
    Invest Ophthalmol Vis Sci 47:3004-10. 2006
    ..To identify new mouse models for studying roles of alphaAlpha-crystallin in vivo and to investigate why and how different mutations of the alphaAlpha-crystallin gene lead to dominant or recessive cataracts...
  48. pmc Exacerbation of retinal degeneration in the absence of alpha crystallins in an in vivo model of chemically induced hypoxia
    Jennifer Yaung
    Department of Pathology, Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA
    Exp Eye Res 86:355-65. 2008
    ..We conclude that lack of alpha-crystallins accentuates retinal degeneration in chemically induced hypoxia in vivo...
  49. ncbi Mechanism of small heat shock protein function in vivo: a knock-in mouse model demonstrates that the R49C mutation in alpha A-crystallin enhances protein insolubility and cell death
    Jing Hua Xi
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:5801-14. 2008
    alphaA-crystallin (Cryaa/HSPB4) is a small heat shock protein and molecular chaperone that prevents nonspecific aggregation of denaturing proteins...
  50. ncbi Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron
    C R King
    Cell 32:707-12. 1983
    ..We propose that the murine alpha A2-crystallin gene generates both the alpha A2 and the alpha Ains mRNAs by alternative splicing...
  51. pmc Severe defects in proliferation and differentiation of lens cells in Foxe3 null mice
    Olga Medina-Martinez
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Biol 25:8854-63. 2005
    ..While Foxe3 is also expressed in a distinct region of the embryonic brain, we have not observed abnormal development of the brain in Foxe3(-/-) animals...
  52. pmc Fibroblast growth factor receptor 1 (Fgfr1) is not essential for lens fiber differentiation in mice
    Haotian Zhao
    Center for Human and Molecular Genetics, Columbus Children s Research Institute, Columbus, OH, USA
    Mol Vis 12:15-25. 2006
    ..These experiments were conducted to determine if Fgfr1 plays an independent, essential role in lens development...
  53. ncbi Foxe3 is required for morphogenesis and differentiation of the anterior segment of the eye and is sensitive to Pax6 gene dosage
    Asa Blixt
    Dept of Cell and Molecular Biology, Goteborg University, 405 30 Goteborg, Sweden
    Dev Biol 302:218-29. 2007
    ..We therefore propose that many of the ocular malformations associated with Pax6 haploinsufficiency are consequences of a reduced expression of Foxe3...
  54. pmc Dosage effects of cohesin regulatory factor PDS5 on mammalian development: implications for cohesinopathies
    Bin Zhang
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA
    PLoS ONE 4:e5232. 2009
    ....
  55. pmc The type I BMP receptors, Bmpr1a and Acvr1, activate multiple signaling pathways to regulate lens formation
    Ramya Rajagopal
    Departments of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63110, USA
    Dev Biol 335:305-16. 2009
    ..A key function of Smad-independent BMP receptor signaling may be reorganization of actin cytoskeleton to drive lens invagination...
  56. pmc Hydroimidazolone modification of human alphaA-crystallin: Effect on the chaperone function and protein refolding ability
    Mahesha H Gangadhariah
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106, USA
    Biochim Biophys Acta 1802:432-41. 2010
    ....
  57. pmc Quantitative biometric phenotype analysis in mouse lenses
    Matthew A Reilly
    Department of Veterans Affairs Medical Center, St Louis, MO 63110, USA
    Mol Vis 16:1041-6. 2010
    ..These methods are robust tools for measuring dry lens weight and quantitatively comparing the growth of small lenses that are difficult to weigh accurately such as those from very young mice and mice with developmental lens defects...
  58. doi Diabetes impairs the neuroprotective properties of retinal alpha-crystallins
    Mandy K Losiewicz
    Department of Ophthalmology, Penn State University, Hershey, Pennsylvania, USA
    Invest Ophthalmol Vis Sci 52:5034-42. 2011
    ..Expression of the proteins of the crystallin superfamily in the retina and their role in neuronal cell survival were investigated in two animal models of diabetes and retinal neurons in culture...
  59. pmc MAPK1 is required for establishing the pattern of cell proliferation and for cell survival during lens development
    Dinesh Upadhya
    Department of Ophthalmology, University of Missouri, Columbia, MO 65212, USA
    Development 140:1573-82. 2013
    ....
  60. pmc The functional role of the Meis/Prep-binding elements in Pax6 locus during pancreas and eye development
    Christian Carbe
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Dev Biol 363:320-9. 2012
    ..Together, these results provide functional evidence for the independent and synergistic roles of the Pax6 upstream enhancers, and they suggest the potential redundancy of Meis/Prep protein in Pax6 regulation...
  61. pmc p19Arf represses platelet-derived growth factor receptor β by transcriptional and posttranscriptional mechanisms
    Ryan C Widau
    Department of Pediatrics, Section of Hematology Oncology and Stem Cell Transplantation, University of Chicago, Chicago, Illinois, USA
    Mol Cell Biol 32:4270-82. 2012
    ....
  62. pmc Chaperone peptides of α-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts
    Rooban B Nahomi
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 288:13022-35. 2013
    ..The results suggest that the α-crystallin chaperone peptides could be used as therapeutic agents to treat cataracts and diseases in which protein aggregation and apoptosis are contributing factors...
  63. ncbi Mapping of six DNA markers on mouse chromosome 17
    J Sertic
    Max Planck Institut fur Biologie, Abteilung Immungenetik, Tubingen, Germany
    Mamm Genome 2:138-42. 1992
  64. pmc New molecular markers for the distal end of the t-complex and their relationships to mutations affecting mouse development
    T Ebersole
    Department of Zoology, University of Texas, Austin 78712 1064
    Genetics 131:175-82. 1992
    ....
  65. ncbi Mapping HSA 3 loci in cattle: additional support for the ancestral synteny of HSA 3 and 21
    D S Threadgill
    Department of Veterinary Pathology, Texas A and M University, College Station 77843
    Genomics 11:1143-8. 1991
    ..FIM3, SI, and CP mapped to bovine syntenic group U10, while RAF1 and GPX1 mapped to U12...
  66. pmc A novel mouse chromosome 17 hybrid sterility locus: implications for the origin of t haplotypes
    S H Pilder
    Department of Molecular Biology, Princeton University, New Jersey 08544 1014
    Genetics 129:237-46. 1991
    ..domesticus background, and (2) t haplotypes would be transmitted at a ratio closer to Mendelian within the M. spretus background.(ABSTRACT TRUNCATED AT 250 WORDS)..
  67. ncbi Regional assignment of the mouse alpha A2-crystallin gene (Crya-1) to chromosome 17A3----B by in situ hybridization
    N W Kaye
    Department of Anatomy, Albany Medical College, NY
    Cytogenet Cell Genet 53:95-6. 1990
    ..In man, however, the homologous gene (CRYA1) is located on human chromosome 21, indicating that internal rearrangements can occur within highly conserved ..
  68. pmc Evolution of mouse chromosome 17 and the origin of inversions associated with t haplotypes
    M F Hammer
    Department of Biology, Princeton University, NJ 08544
    Proc Natl Acad Sci U S A 86:3261-5. 1989
    ..domesticus and M. abbotti, and that the other three inversions occurred on the separate lineage leading to present-day t haplotypes. Alternative models for the evolution of t haplotypes are discussed in light of these findings...
  69. ncbi Linkage of the locus encoding the A chain of alpha-crystallin (Acry-1) to the major histocompatibility complex in the rat
    L C Skow
    Immunogenetics 22:291-3. 1985
  70. pmc Polymorphism and linkage of the alpha A-crystallin gene in t-haplotypes of the mouse
    L C Skow
    Genetics 116:107-11. 1987
    ....
  71. pmc The putative oncogene Pim-1 in the mouse: its linkage and variation among t haplotypes
    J H Nadeau
    Jackson Laboratory, Bar Harbor, Maine 04609
    Genetics 117:533-41. 1987
    ..If these variant alleles can be shown to be identical to the wild-type allele, then lack of association suggests that multiple exchanges have occurred during the evolution of the t complex...
  72. ncbi Chromosomal localization of a new mouse lens opacity gene (lop18)
    B Chang
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Genomics 36:171-3. 1996
    ..showed that this is inherited as a single recessive fully penetrant gene, which we have designated lop18 (lens opacity 18)...
  73. ncbi High-resolution mapping of sperm function defects in the t complex fourth inversion
    A A Redkar
    Temple University School of Medicine, Department of Anatomy and Cell Biology, 3400 North Broad Street, Philadelphia, Pennsylvania 19140, USA
    Mamm Genome 9:825-30. 1998
    ..Thus, these studies provide higher resolution mapping of the molecular basis of t haplotype-specific sperm dysfunction emanating from In(17)4...
  74. ncbi Aphakia (ak), a mouse mutation affecting early eye development: fine mapping, consideration of candidate genes and altered Pax6 and Six3 gene expression pattern
    C Grimm
    GSF National Research Center for Environment and Health, Institute of Mammalian Genetics, Neuherberg, Germany
    Dev Genet 23:299-316. 1998
    ..The expression pattern of Pax2 appeared normal; Cryaa expression could not be detected...
  75. ncbi Identification of a missense mutation in the alphaA-crystallin gene of the lop18 mouse
    B Chang
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Mol Vis 5:21. 1999
    The mouse lop18 (lens opacity 18) mutation causes a white cataract obvious at weaning age. It soon progresses to a large white nuclear cataract with mild cortical changes...
  76. ncbi An axonemal dynein at the Hybrid Sterility 6 locus: implications for t haplotype-specific male sterility and the evolution of species barriers
    J Fossella
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08540, USA
    Mamm Genome 11:8-15. 2000
    ..a flagellar developmental defect, "whipless", to the Hybrid Sterility 6 locus (Hst6), between the markers Pim1 and Crya1. More recent heterospecific breeding experiments between Mus spretus (Spretus) and Mus musculus domesticus (..
  77. ncbi E2F1 mediates ectopic proliferation and stage-specific p53-dependent apoptosis but not aberrant differentiation in the ocular lens of Rb deficient fetuses
    Y Liu
    Department of Medicine, Toronto General Hospital Research Institute, University Health Network, University of Toronto, Ontario, Canada
    Oncogene 19:6065-73. 2000
    ..Thus, inactivation of E2F1 reduces ectopic cell proliferation and stage-specific p53-dependent apoptosis but does not rescue the differentiation defects associated with loss of Rb during lens development...
  78. pmc Mutually regulated expression of Pax6 and Six3 and its implications for the Pax6 haploinsufficient lens phenotype
    Guy Goudreau
    Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Am Fassberg, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:8719-24. 2002
    ..Our findings thus provide a mechanism implicating gene regulatory interactions between Pax6 and Six3 in the tissue-specific defects found in Pax6 heterozygous mice...
  79. ncbi Analysis of non-crystallin lens fiber cell gene expression in c-Maf -/- mice
    Daryle J DePianto
    Department of Cell Biology Human Anatomy, School of Medicine, University of California, Davis 95616, USA
    Mol Vis 9:288-94. 2003
    ..To examine the role of the c-Maf transcription factor in the regulation of non-crystallin lens fiber cell specific gene expression...
  80. ncbi Immunotolerance toward native alphaA-crystallin in knockout mice deficient in the functional protein
    Puwat Charukamnoetkanok
    The National Eye Institute, National Institutes of Health, Bldg 10, Rm 10N112, Bethesda, MD 20892 1857, USA
    Immunol Lett 89:259-65. 2003
    ..These data suggest that nonfunctional proteins may induce immunotolerance and protect recipients of gene therapy from immunity against the native proteins...
  81. ncbi Generation of structures formed by lens and retinal cells differentiating from embryonic stem cells
    Mariko Hirano
    Department of Immunology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan
    Dev Dyn 228:664-71. 2003
    ..The present culture system opens up the possibility of examining early stages of eye development and also of producing cells for use in cellular therapy for various diseases of the eye...
  82. ncbi Heparan sulfate biosynthetic gene Ndst1 is required for FGF signaling in early lens development
    Yi Pan
    Department of Medical and Molecular Genetics, Indiana University of Medicine, Indianapolis, IN 46202, USA
    Development 133:4933-44. 2006
    ..Taken together, these results establish an important role of Ndst1 function in FGF signaling during lens development...
  83. pmc Small heat shock protein alphaA-crystallin regulates epithelial sodium channel expression
    Ossama B Kashlan
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 282:28149-56. 2007
    ..These data are consistent with the hypothesis that small heat shock proteins recognize ENaC subunits at ER quality control checkpoints and can target ENaC subunits for ER-associated degradation...
  84. pmc Eye formation in the absence of retina
    Eric C Swindell
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Dev Biol 322:56-64. 2008
    ..One involved in the formation of the eyeball and the second involved in the formation of the auxiliary eye structures...
  85. pmc Transcriptional regulation of mouse alpha A-crystallin gene in a 148kb Cryaa BAC and its derivates
    Louise Wolf
    The Departments of Ophthalmology and Visual Sciences, Bronx, NY 10461, USA
    BMC Dev Biol 8:88. 2008
    ..However, the onset of transgenic EGFP expression was delayed by 12-24 hours, compared to the expression of the endogenous Cryaa gene.
  86. ncbi Changes in physicochemical parameters and alpha-crystallin expression in the lens during cataract development in OXYS rats. 5
    Yu V Rumyantseva
    Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia
    Biochemistry (Mosc) 73:1176-82. 2008
    ..The changes observed in OXYS rats are evidently associated with the dystrophic changes in lens epithelium, which we have described earlier, and are consistent with the model of senile cataract...
  87. pmc Pitx3 controls multiple aspects of lens development
    Olga Medina-Martinez
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Dev Dyn 238:2193-201. 2009
    ..In general, our study shows that the abnormal phenotype of the ak lenses is not due to just one molecular pathway, rather in the absence of Pitx3 expression multiple aspects of lens development are disrupted...
  88. pmc αA-Crystallin associates with α6 integrin receptor complexes and regulates cellular signaling
    A Sue Menko
    Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson School of Medicine, Philadelphia, PA 19107 6799, USA
    Exp Eye Res 91:640-51. 2010
    ..The observed changes in lens cell signaling likely reflect long-term functional adaptations to the absence of the αA-crystallin chaperone/small heat-shock protein...
  89. pmc Physiological expression of lens α-, β-, and γ-crystallins in murine and human corneas
    Shengwei Ren
    Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
    Mol Vis 16:2745-52. 2010
    ..This study investigated whether the three classical lens crystallins, namely α-, β-, and γ-crystallins, exist in mouse and human corneas...
  90. pmc Cell proliferation in the absence of E2F1-3
    Pamela L Wenzel
    Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA
    Dev Biol 351:35-45. 2011
    ..Together, these data implicate E2F1-3 in mediating transcriptional repression by Rb during cell cycle exit and point to a critical role for their repressive functions in cell survival...
  91. pmc Conditional disruption of mouse Klf5 results in defective eyelids with malformed meibomian glands, abnormal cornea and loss of conjunctival goblet cells
    Doreswamy Kenchegowda
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Dev Biol 356:5-18. 2011
    ..Thus, Klf5CN mice provide a useful model for investigating ocular surface pathologies involving meibomian gland dysfunction, blepharitis, corneal or conjunctival defects...
  92. ncbi Map positions of four dinucleotide repeats in the mouse t complex
    F Lai
    Department of Zoology, University of Texas, Austin 78712 1064
    Mamm Genome 3:476-7. 1992
  93. ncbi One subunit of the transcription factor NF-Y maps close to the major histocompatibility complex in murine and human chromosomes
    X Y Li
    LGME du CNRS, Faculte de Medecine, Strasbourg, France
    Genomics 11:630-4. 1991
    ..NF-YA is assigned to human chromosome 6p21 and to mouse chromosome 17. NF-YB is assigned to human chromosome 12 and to mouse chromosome 10...
  94. ncbi Localization of a mouse heat shock Hsp70 gene within the H-2 complex
    H R Gaskins
    Jackson Laboratory, Bar Harbor, ME 04609
    Immunogenetics 32:286-9. 1990
  95. ncbi The alpha-A-crystallin and cystathionine beta-synthase genes are physically very closely linked in proximal mouse chromosome 17
    L Stubbs
    Imperial Cancer Research Fund Laboratories, Lincoln s Inn Fields, London, United Kingdom
    Genomics 7:284-8. 1990
    ....
  96. pmc The locus encoding alpha A-crystallin is closely linked to H-2K on mouse chromosome 17
    L C Skow
    Genetics 110:723-32. 1985
    ..Analysis of congenic and recombinant congenic strains of mice confirmed the linkage of Acry-1 and H-2 and located the alpha A gene to the region between glyoxylase (Glo-1) and H-2K...
  97. pmc Transcriptional regulation of the mouse alpha A-crystallin gene: activation dependent on a cyclic AMP-responsive element (DE1/CRE) and a Pax-6-binding site
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730
    Mol Cell Biol 15:653-60. 1995
    ..These data provide evidence for a synergistic role of Pax-6 and CREB-like proteins for high expression of the mouse alpha A-crystallin gene in the lens...
  98. pmc Contrasting roles for c-Myc and L-Myc in the regulation of cellular growth and differentiation in vivo
    S D Morgenbesser
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461
    EMBO J 14:743-56. 1995
    ..As a direct corollary, our data indicate that L-Myc and c-Myc are involved in distinct physiological processes in the same cell type...
  99. ncbi Identification of negative-acting and protein-binding elements in the mouse alpha A-crystallin -1556/-1165 region
    C M Sax
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 144:163-9. 1994
    ..We demonstrate that the chicken delta 1-cry intron repressor binds similar nuclear proteins in chicken embryonic lens cells and mouse alpha TN4-1 lens cells.(ABSTRACT TRUNCATED AT 250 WORDS)..
  100. ncbi Hybrid sterility-6: a mouse t complex locus controlling sperm flagellar assembly and movement
    S H Pilder
    Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140
    Dev Biol 159:631-42. 1993
    ..Thus, Hst-6 provides direct access to the molecular basis of t haplotype-specific alterations in sperm function that emanate from the t complex distal inversion...
  101. ncbi FGF suppresses apoptosis and induces differentiation of fibre cells in the mouse lens
    R L Chow
    Skirball Institute for Biomolecular Medicine, New York University Medical Center, NY 10016, USA
    Development 121:4383-93. 1995
    ..These results show that lens fibre cells are dependent on FGF for their survival and differentiation, and demonstrate that growth factor deprivation in vivo can lead to apoptosis...

Research Grants1

  1. Confocal Core Facility for Vision Researchers
    A Menko; Fiscal Year: 2007
    ..abstract_text> ..