Aprt

Summary

Gene Symbol: Aprt
Description: adenine phosphoribosyl transferase
Alias: C85684, adenine phosphoribosyltransferase
Species: mouse

Top Publications

  1. pmc SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair
    Berta N Vazquez
    Department of Genetics, Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA
    EMBO J 35:1488-503. 2016
  2. pmc Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress
    Vamsi Rani
    Department of Genetics and the Human Genetics Institute of New Jersey, Rutgers University, 145 Bevier Road, Piscataway, NJ 08854, USA
    Mutat Res 735:39-45. 2012
  3. ncbi In vivo loss of heterozygosity in T-cells of B6C3F1 Aprt(+/-) mice
    L Liang
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN, USA
    Environ Mol Mutagen 35:150-7. 2000
  4. ncbi Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2
    U Francke
    Cytogenet Cell Genet 19:57-84. 1977
  5. ncbi The mouse N-acetylgalactosamine-6-sulfate sulfatase (Galns) gene: cDNA isolation, genomic characterization, chromosomal assignment and analysis of the 5'-flanking region
    A M Montano
    Department of Pediatrics, Gifu University School of Medicine, 40 Tsukasa Machi, Gifu, Japan
    Biochim Biophys Acta 1500:323-34. 2000
  6. ncbi Solid tissues removed from ATM homozygous deficient mice do not exhibit a mutator phenotype for second-step autosomal mutations
    M S Turker
    Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland 97201, USA
    Cancer Res 59:4781-3. 1999
  7. ncbi Embryonic stem cells can be used to construct hybrid cell lines containing a single, selectable murine chromosome
    P M Jakobs
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, 3181 S W Sam Jackson Park Road, Portland, Oregon 97201, USA
    Mamm Genome 10:381-4. 1999
  8. pmc The primary function of a redundant Sp1 binding site in the mouse aprt gene promoter is to block epigenetic gene inactivation
    P Mummaneni
    Department of Pathology, University of Kentucky, Lexington, KY 40536, USA
    Nucleic Acids Res 26:5163-9. 1998
  9. pmc Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice
    P P Van Sloun
    Department of Radiation Genetics and Chemical Mutagenesis MGC, Leiden University Medical Center, PO Box 9503, 2300 RA Leiden, The Netherlands
    Nucleic Acids Res 26:4888-94. 1998
  10. ncbi Chronic renal failure in a mouse model of human adenine phosphoribosyltransferase deficiency
    M G Stockelman
    Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267, USA
    Am J Physiol 275:F154-63. 1998

Scientific Experts

  • MITCHELL TURKER
  • Changshun Shao
  • Andrew E Evan
  • Jay A Tischfield
  • Li Liang
  • Berta N Vazquez
  • Phillip A Yates
  • J A Tischfield
  • Vamsi Rani
  • Cristian Dan
  • P J Stambrook
  • Jon A Oyer
  • Eleni G Tzortzaki
  • Julio Barrera-Oro
  • Hilary J Vernon
  • S W Wijnhoven
  • Marc S Mendonca
  • Li Deng
  • Jiaqiang Huang
  • Amrik Sahota
  • Sharon B Bledsoe
  • Min Yang
  • Nicolas G Simonet
  • Samuel Bunting
  • Lourdes Serrano
  • Paloma Martínez-Redondo
  • Joshua K Thackray
  • Trang Nguyen
  • Noriko Kane-Goldsmith
  • Alejandro Vaquero
  • S J Engle
  • Peter J Stambrook
  • P Mummaneni
  • M G Stockelman
  • Carola A Neumann
  • Sarah Godsey
  • Michael Lasarev
  • Marissa Connolly
  • Brittany Cross
  • Amy Kronenberg
  • Dmytro Grygoryev
  • Kelly Sandfort
  • L Liang
  • A M Montano
  • A Sahota
  • J A Rose
  • R Slater
  • A A van Zeeland
  • H Vrieling
  • H J Kool
  • J Simpson
  • Raju Kucherlapati
  • Tzu Yang Liu
  • P M Jakobs
  • Erin Gorden
  • M Cool
  • Son C Nguyen
  • M Kido
  • P P Van Sloun
  • J Becker-Follmann
  • N H Khattar
  • David T Denhardt
  • Christine Osborne
  • Susan R Rittling
  • Lynette Fairbanks
  • H Anne Simmonds
  • Jianmen Chen
  • Steven Buyske
  • M Yadollahi-Farsani
  • Heming Zhu
  • Tao Fan
  • Kathrin Muegge
  • Stephen Fox
  • Lisa Gangi
  • Lionel Best
  • Haleem J Issaq
  • Qingsheng Yan
  • David Munroe
  • Robert Burman
  • James Simpson
  • Mathew J Thayer
  • Olga N Ponomoreva
  • P M Davies
  • M L Guevara-Fujita
  • H A Simmonds
  • N G Copeland
  • G Boivin
  • N J Redhead
  • N A Jenkins
  • Dayna Glass

Detail Information

Publications66

  1. pmc SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair
    Berta N Vazquez
    Department of Genetics, Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA
    EMBO J 35:1488-503. 2016
    ..These results reveal a direct role for SIRT7 in DSB repair and establish a functional link between SIRT7-mediated H3K18 deacetylation and the maintenance of genome integrity. ..
  2. pmc Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress
    Vamsi Rani
    Department of Genetics and the Human Genetics Institute of New Jersey, Rutgers University, 145 Bevier Road, Piscataway, NJ 08854, USA
    Mutat Res 735:39-45. 2012
    ..Using Aprt as a LOH reporter, we observed an elevation in LOH mutation frequency in fibroblasts, but not in T cells, of Prdx1(-..
  3. ncbi In vivo loss of heterozygosity in T-cells of B6C3F1 Aprt(+/-) mice
    L Liang
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN, USA
    Environ Mol Mutagen 35:150-7. 2000
    We have used B6C3F1 mice heterozygous at Aprt (adenine phosphoribosyltransferase) as a model to study in vivo loss of heterozygosity (LOH) in normal splenic T-lymphocytes...
  4. ncbi Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2
    U Francke
    Cytogenet Cell Genet 19:57-84. 1977
    ..They also confirm chromosomally the assignments of 3 genes that were made by other somatic cell genetic studies: Aprt to 8; Hprt and alpha-gal to the X chromosome...
  5. ncbi The mouse N-acetylgalactosamine-6-sulfate sulfatase (Galns) gene: cDNA isolation, genomic characterization, chromosomal assignment and analysis of the 5'-flanking region
    A M Montano
    Department of Pediatrics, Gifu University School of Medicine, 40 Tsukasa Machi, Gifu, Japan
    Biochim Biophys Acta 1500:323-34. 2000
    ..was mapped by interspecific backcross analysis to the distal region of chromosome 8 where it co-segregates with Aprt. Northern blot analysis showed a wide expression of a single-copy gene, being higher especially in liver and kidney...
  6. ncbi Solid tissues removed from ATM homozygous deficient mice do not exhibit a mutator phenotype for second-step autosomal mutations
    M S Turker
    Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland 97201, USA
    Cancer Res 59:4781-3. 1999
    ..We have bred mice with knockout mutations for the selectable Aprt (adenine phosphoribosyltransferase) locus and the Atm locus to examine the frequency of second-step autosomal mutations in Atm-..
  7. ncbi Embryonic stem cells can be used to construct hybrid cell lines containing a single, selectable murine chromosome
    P M Jakobs
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, 3181 S W Sam Jackson Park Road, Portland, Oregon 97201, USA
    Mamm Genome 10:381-4. 1999
    ..ES cells with mutations in aprt, fyn, and myc were utilized to generate monochromosomal hybrids with neomycin phosphotransferase-marked murine Chr ..
  8. pmc The primary function of a redundant Sp1 binding site in the mouse aprt gene promoter is to block epigenetic gene inactivation
    P Mummaneni
    Department of Pathology, University of Kentucky, Lexington, KY 40536, USA
    Nucleic Acids Res 26:5163-9. 1998
    The promoter region of the mouse adenine phosphoribosyltransferase (aprt) gene contains one non-consensus Sp1 binding site at its 5' end followed by three consensus Sp1 binding sites...
  9. pmc Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice
    P P Van Sloun
    Department of Radiation Genetics and Chemical Mutagenesis MGC, Leiden University Medical Center, PO Box 9503, 2300 RA Leiden, The Netherlands
    Nucleic Acids Res 26:4888-94. 1998
    A mouse model was generated to investigate loss of heterozygosity (LOH) events in somatic cells. The adenine phosphoribosyltransferase ( Aprt ) gene was disrupted in embryonic stem cells using a conventional gene targeting approach and ..
  10. ncbi Chronic renal failure in a mouse model of human adenine phosphoribosyltransferase deficiency
    M G Stockelman
    Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267, USA
    Am J Physiol 275:F154-63. 1998
    In humans, adenine phosphoribosyltransferase (APRT, EC 2.4.2.7) deficiency can manifest as nephrolithiasis, interstitial nephritis, and chronic renal failure...
  11. ncbi Molecular properties and chromosomal location of cadherin-8
    M Kido
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA
    Genomics 48:186-94. 1998
    ..Cadherin-8, as well as cadherin-11, was mapped to a specific region of chromosome 8 that also includes cadherins-1, -3, and -5...
  12. ncbi Molecular mechanisms of mutagen hypersensitivity in adenine phosphoribosyl transferase-deficient Friend mouse erythroleukaemia cells
    M Yadollahi-Farsani
    School of Biomedical Sciences, University of Ulster, Coleraine, Co Londonderry, Northern Ireland, UK
    Br J Biomed Sci 54:174-80. 1997
    Deficiency of the enzyme adenine phosphoribosyltransferase (APRT) has been associated with hypersensitivity to the mutagenic effects of ethyl methanesulphonate (EMS) and 254 nm ultraviolet (UV) radiation in clone 707 of Friend mouse ..
  13. ncbi A role for certain mouse Aprt sequences in resistance to toxic adenine analogs
    N H Khattar
    Department of Microbiology and Immunology, University of Kentucky College of Medicine, Lexington 40536, USA
    Somat Cell Mol Genet 23:51-61. 1997
    A mouse embryonal carcinoma cell line hemizygous for the adenine phosphoribosyltransferase gene (aprt) was exposed to ultraviolet light (UV) or to the alkylating agent, ethyl methanesulfonate (EMS)...
  14. ncbi Molecular evidence for the induction of large interstitial deletions on mouse chromosome 8 by ionizing radiation
    M S Turker
    Dept of Pathology, University of Kentucky, Lexington 40536, USA
    Mutat Res 374:201-8. 1997
  15. ncbi HPRT-APRT-deficient mice are not a model for lesch-nyhan syndrome
    S J Engle
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202 5251, USA
    Hum Mol Genet 5:1607-10. 1996
    ..Administration of drugs that inhibit adenine phosphoribosyltransferase (APRT) in HPRT-deficient mice has produced the suggestion that deficiency of APRT in combination ..
  16. ncbi Mice with adenine phosphoribosyltransferase deficiency develop fatal 2,8-dihydroxyadenine lithiasis
    N J Redhead
    Institute of Cell and Molecular Biology, University of Edinburgh, Scotland
    Hum Gene Ther 7:1491-502. 1996
    ..that this may be due to a greater dependency in rodents on the other purine salvage enzyme, adenine phosphoribosyltransferase (APRT)...
  17. ncbi Chromosomal assignment of seven genes on canine chromosomes by fluorescence in situ hybridization
    M L Guevara-Fujita
    Department of Human Genetics, University of Michigan Medical School, 4708 Medical Science Building II, Ann Arbor, Michigan 48109 0618, USA
    Mamm Genome 7:268-70. 1996
    ..These assignments are based on the karyotype proposed by Stone and associates (Genome 34, 407, 1991) using high-resolution techniques. In addition, we have assigned the Menkes gene to the X Chr of the dog...
  18. pmc Chromosome instability contributes to loss of heterozygosity in mice lacking p53
    C Shao
    Department of Genetics, Rutgers, The State University of New Jersey, 604 Allison Road, Piscataway, NJ 08854 8082, USA
    Proc Natl Acad Sci U S A 97:7405-10. 2000
    ..to genetic instability in normal cells in vivo, we have examined mechanisms of loss of heterozygosity (LOH) at the Aprt (adenine phosphoribsyltransferase) and flanking loci in normal fibroblasts and T lymphocytes of p53-deficient mice...
  19. ncbi Biallelic methylation and silencing of mouse Aprt in normal kidney cells
    J A Rose
    Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland 97201, USA
    Cancer Res 60:3404-8. 2000
    ..In the present study, we show that methylation-associated silencing of the autosomal adenine phosphoribosyltransferase (Aprt) locus occurs in primary mouse kidney cells...
  20. ncbi DMBA-induced toxic and mutagenic responses vary dramatically between NER-deficient Xpa, Xpc and Csb mice
    S W Wijnhoven
    Department of Radiation Genetics and Chemical Mutagenesis MGC, Leiden University Medical Center, The Netherlands
    Carcinogenesis 22:1099-106. 2001
    ..for the variability in cancer proneness, we studied mutagenesis at the X-chromosomal Hprt gene and the autosomal Aprt gene in splenic T-lymphocytes after 7,12-dimethyl-1,2-benz[a]anthracene (DMBA) exposure in total NER-deficient Xpa ..
  21. ncbi Sequential analysis of kidney stone formation in the Aprt knockout mouse
    A P Evan
    Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202 5120, USA
    Kidney Int 60:910-23. 2001
    We have previously shown that, as in human adenine phosphoribosyltransferase (APRT) deficiency, Aprt knockout mice form 2,8-dihydroxyadenine (DHA) renal stones. The disease develops earlier and is more severe in male than in female mice...
  22. ncbi Radiation-induced genetic instability in vivo depends on p53 status
    Li Liang
    Department of Genetics, State University of New Jersey, Rutgers, Piscataway, NJ 08854, USA
    Mutat Res 502:69-80. 2002
    ..stability in normal tissues following irradiation, we have measured mutant frequencies at the adenine phosphoribosyltransferase (Aprt) and hypothanine-guanine phosphoribosyltransferase (Hprt) loci and examined mechanisms of loss ..
  23. ncbi Gender- and age-dependent changes in kidney androgen protein mRNA expression in a knockout mouse model for nephrolithiasis
    Eleni G Tzortzaki
    Department of Genetics, Rutgers University, Piscataway, New Jersey 08854, USA
    J Histochem Cytochem 50:1663-9. 2002
    ..observed a significant decrease in Kap mRNA expression in whole kidney tissue from male mice with adenine phosphoribosyltransferase (APRT) deficiency and 2,8-dihydroxyadenine (DHA) nephrolithiasis...
  24. pmc Silencing of mouse Aprt is a gradual process in differentiated cells
    Phillip A Yates
    Center for Research on Occupational and Environmental Toxicology, Oregon Health and Sciences University, Portland, Oregon 97239, USA
    Mol Cell Biol 23:4461-70. 2003
    Mouse Aprt constructs that are highly susceptible to DNA methylation-associated inactivation in embryonal carcinoma cells were transfected into differentiated cells, where they were expressed...
  25. pmc Lsh, an epigenetic guardian of repetitive elements
    Jiaqiang Huang
    Laboratory of Molecular Immunoregulation, SAIC Basic Research Program, National Cancer Institute, Frederick, MD 21701, USA
    Nucleic Acids Res 32:5019-28. 2004
    ..These data suggest that the repetitive sites are direct targets of Lsh action and that Lsh plays an important role as 'epigenetic guardian' of the genome to protect against deregulation of parasitic retroviral elements...
  26. ncbi Mlh1 mediates tissue-specific regulation of mitotic recombination
    Changshun Shao
    Department of Genetics, Rutgers University, 604 Allison Road, Piscataway, NJ 08854 8082, USA
    Oncogene 23:9017-24. 2004
    ..The frequency of point mutations at the reporter gene loci Aprt and Hprt, on the other hand, is significantly increased in both T cells and fibroblasts of Mlh1(-/-) mice...
  27. ncbi Aprt/Opn double knockout mice: osteopontin is a modifier of kidney stone disease severity
    Hilary J Vernon
    Department of Genetics, Rutgers University, Piscataway, New Jersey 08854 8082, USA
    Kidney Int 68:938-47. 2005
    ..We examined the role of OPN in adenine phosphoribosyltransferase (Aprt) knockout mice, in which inflammation and formation of 2,8-dihydroxyadenine (DHA) kidney ..
  28. ncbi Reduced apoptosis and increased deletion mutations at Aprt locus in vivo in mice exposed to repeated ionizing radiation
    Li Liang
    Department of Genetics, Rutgers University, 604 Allison Road, Piscataway, NJ 08854, USA
    Cancer Res 67:1910-7. 2007
    ..However, a single 4-Gy whole body X-ray exposure only induced a modest increase of mutations at the Aprt reporter gene locus in mouse T cells...
  29. pmc Role of the mismatch repair gene, Msh6, in suppressing genome instability and radiation-induced mutations
    Julio Barrera-Oro
    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Mutat Res 642:74-9. 2008
    ..between MSH6-proficient and deficient, 129S2xC57BL/6 F1 hybrid mice that were heterozygous for our reporter gene Aprt. We recovered mutant cells that had functionally lost APRT protein activity and categorized the spectrum of ..
  30. pmc Mutagenesis in vivo in T cells of p21-deficient mice
    Changshun Shao
    Department of Genetics, Rutgers University, Piscataway, NJ 08854, USA
    Mutat Res 670:103-6. 2009
    ..study, we characterized mutagenesis in vivo in T cells of p21-deficient mice, using loss of heterozygosity (LOH) at Aprt locus as an indicator...
  31. pmc Aberrantly silenced promoters retain a persistent memory of the silenced state after long-term reactivation
    Jon A Oyer
    Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
    Mutat Res 706:21-7. 2011
    ..issue, spontaneous reactivant clones were isolated from mouse embryonal carcinoma cells bearing aberrantly silenced Aprt alleles and re-silencing frequencies were determined as long as three months after reactivation occurred...
  32. pmc Marked aneuploidy and loss of multiple chromosomes are common in autosomal mutants isolated from normal mouse kidney epithelium
    Cristian Dan
    Center for Research on Occupational and Environmental Toxicology, Oregon Health and Sciences University, Portland, OR 97239, USA
    Genes Chromosomes Cancer 50:239-49. 2011
    ..isolated directly from normal kidney epithelium arose from loss of a marker chromosome 8 containing the wild type Aprt gene. Chromosome loss was detected by loss of heterozygosity (LOH) for all chromosome 8 polymorphic loci examined...
  33. pmc Genomic organization and chromosomal mapping of the mouse P-cadherin gene
    M Hatta
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Nucleic Acids Res 19:4437-41. 1991
    ..This is the first evidence for the linkage of different cadherin genes...
  34. pmc Assignment of the gene for adenine phosphoribosyltransferase to human chromosome 16 by mouse-human somatic cell hybridization
    J A Tischfield
    Proc Natl Acad Sci U S A 71:45-9. 1974
    A series of mouse-human hybrids was prepared from mouse cells deficient in adenine phosphoribosyltransferase (EC 2.4.2.7) and normal human cells...
  35. pmc Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement
    M K Dush
    Proc Natl Acad Sci U S A 82:2731-5. 1985
    We have determined the nucleotide sequence of a functional mouse adenine phosphoribosyltransferase (APRT) gene and its cDNA...
  36. ncbi Assignment of the gene for adenine phosphoribosyltransferase on the genetic map of mouse chromosome 8
    T B Nesterova
    Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk
    Biochem Genet 25:563-8. 1987
    Two electrophoretic variants of adenine phosphoribosyltransferase (APRT) were identified in a population of wild mice (Mus musculus bactrianus)...
  37. ncbi [Localization of the gene coding for adenine phosphoribosyltransferase on the genetic map of chromosome 8 in the mouse]
    T B Nesterova
    Genetika 24:829-35. 1988
    Polymorphism of electrophoretic mobility of adenine phosphoribosyltransferase (APRT) was found in a population of domestic mice, Mus musculus bactrianus...
  38. ncbi Allelic variation linked to adenine phosphoribosyltransferase locus in mouse teratocarcinoma cell line and feral-derived mouse strains
    M S Turker
    Department of Pathology, University of Washington, Seattle 98195
    Somat Cell Mol Genet 15:159-66. 1989
    Southern blot analysis reveals two distinct adenine phosphoribosyltransferase (APRT) alleles in the P-19 mouse teratocarcinoma cell line...
  39. ncbi Assignment of a processed mouse Aprt pseudogene to the same chromosome as the functional gene
    R A Farber
    Cytogenet Cell Genet 42:198-201. 1986
    A novel genetic system has been used to demonstrate that a processed adenine phosphoribosyltransferase (Aprt) pseudogene is located on mouse chromosome 8, which is the same chromosome that carries the functional Aprt gene...
  40. ncbi Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q
    G Scherer
    Institute of Human Genetics, Freiburg, Federal Republic of Germany
    Genomics 5:275-82. 1989
    ..defined by the Hp-Tat interval, by the 24-cM interval between Mt-1,-2 and the conserved locus for adenine phosphoribosyltransferase, Aprt, mapped at 25 cM from Es-1 by T. B. Nesterova, P. M. Borodin, S. M. Zakian, and O. L...
  41. ncbi An interspecific backcross linkage map of mouse chromosome 8
    J D Ceci
    Mammalian Genetics Laboratory, NCI Frederick Cancer Research Facility, Maryland 21701
    Genomics 6:72-9. 1990
    ..Genes that were mapped in this study include Act-6, Aprt, Aprt-ps1, Emv-2, Es-N, Hp, Insr, Mt-1, Plat, Psx-8, Ucp, and Zfp-4...
  42. ncbi Cloning and expression of a mouse adenine phosphoribosyltransferase gene
    J M Sikela
    Gene 22:219-28. 1983
    A functional mouse adenine phosphoribosyltransferase (APRT) gene was identified and cloned by screening a mouse sperm genomic DNA library in lambda Charon 4A...
  43. ncbi Characterization of a murine cDNA encoding a member of the carboxylesterase multigene family
    M Ovnic
    Institute for Developmental Research, Children s Hospital Medical Center, Cincinnati, Ohio 45229
    Genomics 9:344-54. 1991
    ..5 kDa. Upon the addition of microsomes, this product was processed into a protein of 60.4 kDa, which is within the size range of monomeric units of mouse carboxylesterases...
  44. ncbi A linkage map of mouse chromosome 8: further definition of homologous linkage relationships between mouse chromosome 8 and human chromosomes 8, 16, and 19
    T A Howard
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
    Genomics 10:207-13. 1991
    ..5 cM-Plat-26.3 cM-Crryps/Mel/Jund-3.5 cM-Junb/Ucp-10.5 cM-Mt-1-27.2 cM-Acta2-0.9 cM-Aprt. These data provide further definition of mouse chromosome 8 linkage relationships and the relationship between ..
  45. pmc Growth factor-induced delayed early response genes
    A Lanahan
    Howard Hughes Medical Institute, Baltimore, Maryland
    Mol Cell Biol 12:3919-29. 1992
    ..Among the former are cDNAs encoding nonhistone chromosomal proteins [HMGI(Y) and HMGI-C], adenine phosphoribosyltransferase (APRT), a protein related to human macrophage migration inhibitory factor (MIF), a protein of the ..
  46. ncbi At least two distinct epigenetic mechanisms are correlated with high-frequency "switching" for APRT phenotypic expression in mouse embryonal carcinoma stem cells
    G E Cooper
    Department of Microbiology and Immunology, University of Kentucky College of Medicine, Lexington 40536
    Somat Cell Mol Genet 18:215-25. 1992
    A series of clones displaying high frequency "switching" phenotypes for expression of the adenine phosphoribosyltransferase (aprt) gene were previously isolated from the P19 mouse embryonal carcinoma stem cell line...
  47. ncbi The gene for the cell adhesion molecule M-cadherin maps to mouse chromosome 8 and human chromosome 16q24.1-qter and is near the E-cadherin (uvomorulin) locus in both species
    K Kaupmann
    Developmental Biology Unit, University of Bielefeld, Germany
    Genomics 14:488-90. 1992
    ..The gene order cen-Junb-Um-Tat-(Cdh3/Aprt) was determined. The human homologue CDH3 was mapped to chromosome 16q24...
  48. ncbi Tcrb-V3+ T-cell deletion and a mouse mammary tumor provirus, Mtv-27
    K Tomonari
    Transplantation Biology Section, MRC Clinical Research Centre, Harrow, UK
    Immunogenetics 36:302-5. 1992
    ..BR] mice, and show that Mtv-27 as well as Mtv-3 from NZB mice co-segregate with genes encoding deletion ligands for Tcrb-V3+ T cells without recombination...
  49. ncbi Gene linkage analysis in the mouse by somatic cell hybridization: assignment of adenine phosphoribosyltransferase to chromosome 8 and alpha-galactosidase to the X chromosome
    C Kozak
    Somatic Cell Genet 1:371-82. 1975
    ..phosphate isomerase (MPI) segregated concordantly, confirming an established linkage relationship; adenine phosphoribosyltransferase (APRT) segregated concordantly with glutathione reductase (GR) which is known to be on chromosome 8; ..
  50. ncbi Identification of aprt gene mutations induced in repair-deficient and P450-expressing CHO cells by the food-related mutagen/carcinogen, PhIP
    R W Wu
    Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551 0808, USA
    Carcinogenesis 16:1207-13. 1995
    ..Sequence analysis of the PhIP-induced mutations in the adenine phosphoribosyltransferase (aprt) gene, which is heterozygous in the UV5P3 cells, can provide insight into the mutagenic ..
  51. ncbi Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice
    C L Wu
    Institute of Cell and Molecular Biology, Edinburgh University, Scotland
    Nat Genet 3:235-40. 1993
    ..We examined whether mice were more tolerant of HPRT deficiency because they were more reliant on adenine phosphoribosyltransferase (APRT) than HPRT for their purine salvage...
  52. ncbi Region-specific rates of molecular evolution: a fourfold reduction in the rate of accumulation of "silent" mutations in transcribed versus nontranscribed regions of homologous DNA fragments derived from two closely related mouse species
    M S Turker
    Department of Pathology, University of Kentucky College of Medicine, Lexington 40536
    J Mol Evol 36:31-40. 1993
    ..Each DNA fragment contains 1.35 kbp of the 3' end of the constitutively expressed 2.2-kbp aprt (adenine phosphoribosyltransferase) gene and a similarly sized nontranscribed region downstream of the aprt gene...
  53. ncbi Re-localization of Actsk-1 to mouse chromosome 8, a new region of homology with human chromosome 1
    S Alonso
    Unité de Génétique Moléculaire du Développement, URA 1148, Institut Pasteur, Paris, France
    Mamm Genome 4:15-20. 1993
    ..7 cM-Tat-8.3 cM-Actsk-1-0.5 cM-Aprt. Mapping of ACTSK to human Chr 1 and of TAT and APRT to human Chr 16 demonstrates the existence of a new short ..
  54. ncbi A cis-acting element accounts for a conserved methylation pattern upstream of the mouse adenine phosphoribosyltransferase gene
    P Mummaneni
    Department of Pathology, University of Kentucky College of Medicine, Lexington 40536
    J Biol Chem 268:552-8. 1993
    A 2.1-kilobase pair region located just upstream of the mouse aprt (adenine phosphoribosyltransferase) gene has a methylation pattern that is conserved in mouse tissues and culture cell lines...
  55. ncbi mRNA for cardiac calcium channel is expressed during development of skeletal muscle
    N Chaudhari
    Department of Physiology, Colorado State University, Fort Collins 80523
    Dev Biol 155:507-15. 1993
    ..These results raise the question of whether the cardiac calcium channel may be functionally important during the early development of skeletal myofibers...
  56. pmc The genomic structure of the murine ICSBP gene reveals the presence of the gamma interferon-responsive element, to which an ISGF3 alpha subunit (or similar) molecule binds
    Y Kanno
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:3951-63. 1993
    ....
  57. ncbi Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage
    J P Abonia
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, New York State Department of Health, Buffalo 14263
    Mamm Genome 4:25-32. 1993
    ..Analysis of the segregation patterns closely linked Agt to Aprt and Emv-2, which places the angiotensinogen locus on the distal end of mouse Chr 8...
  58. pmc Adenine phosphoribosyltransferase-deficient mice develop 2,8-dihydroxyadenine nephrolithiasis
    S J Engle
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, 46202 5251, USA
    Proc Natl Acad Sci U S A 93:5307-12. 1996
    b>Adenine phosphoribosyltransferase (APRT) deficiency in humans is an autosomal recessive syndrome characterized by the urinary excretion of adenine and the highly insoluble compound 2,8-dihydroxyadenine (DHA) that can produce kidney ..
  59. ncbi Mice lacking ADPRT and poly(ADP-ribosyl)ation develop normally but are susceptible to skin disease
    Z Q Wang
    Research Institute of Molecular Pathology IMP, Vienna, Austria
    Genes Dev 9:509-20. 1995
    ....
  60. ncbi Hemidemethylation is sufficient for chromatin relaxation and transcriptional activation of methylated aprt gene in mouse P19 embryonal carcinoma cell line
    G E Cooper
    Department of Microbiology and Immunology, University of Kentucky College of Medicine, Lexington 40536
    Somat Cell Mol Genet 19:221-9. 1993
    A series of clones displaying a high-frequency "switching" phenotype for expression of the adenine phosphoribosyltransferase (aprt) gene was previously isolated from the P19 mouse embryonal carcinoma stem cell line...
  61. ncbi Spontaneous and ionizing radiation induced mutations involve large events when selecting for loss of an autosomal locus
    M Turker
    Department of Pathology, University of Kentucky College of Medicine, Lexington 40536, USA
    Mutat Res 329:97-105. 1995
    ..musculus). It also contains a deletion for the M. musculus aprt allele, which is located on chromosome 8...
  62. ncbi Assignment of the gene for cytoplasmic glutamate-oxaloacetate transaminase to mouse chromosome 19 using chinese hamster x mouse somatic cell hybrids
    R Schafer
    Somatic Cell Genet 6:709-17. 1980
    ..The appearance of a heteropolymeric band in hybrid cells expressing mouse GOT-1 suggests that this enzyme molecule is probably composed of two subunits...
  63. ncbi Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 7
    R L Stallings
    Somatic Cell Genet 7:683-98. 1981
    ..The assignments provide markers for the study of the genetic consequences of chromosomal rearrangements in Chinese hamster cell lines and support the concept of conservation of mammalian autosomal linkage groups...
  64. pmc Assignment of genes encoding metallothioneins I and II to Chinese hamster chromosome 3: evidence for the role of chromosome rearrangement in gene amplification
    R L Stallings
    Mol Cell Biol 4:2932-6. 1984
    ..We speculate that rearrangement of chromosome 3p could be causally involved with the amplification of MT genes in Cdr hamster cell lines...
  65. ncbi Elevated frequency of loss of heterozygosity in mammary tumors arising in mouse mammary tumor virus/neu transgenic mice
    M Cool
    Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada
    Cancer Res 59:2438-44. 1999
    ....
  66. ncbi High-resolution mapping of a linkage group on mouse chromosome 8 conserved on human chromosome 16Q
    J Becker-Follmann
    Institute of Human Genetics, University of Freiburg, Breisacherstr 33, D 79106 Freiburg, Germany
    Mamm Genome 8:172-7. 1997
    ..6-Zfp1,+ ++Ctrb-10.9-e. In a separate interspecific cross involving 62 meioses, Dpep1 was mapped together with Aprt and Cdh3 at 12.9 cM distal to Hp, Tat, to the vicinity of e...

Research Grants9

  1. SPECTRUM OF MUTATION INDUCED BY IONIZING RADIATION
    MITCHELL TURKER; Fiscal Year: 2002
    ..The autosomal aprt (adenine phosphoribosyltransferase) gene will e used as the mutational target for these studies...
  2. Gene Silencing in Cancer--A Model System
    MITCHELL TURKER; Fiscal Year: 2003
    ..The major goal of this application is to use mouse Aprt to demonstrate that promoter region methylation is the endpoint of a multistep silencing process...
  3. Autosomal Mutagenesis and Polycystic Kidney Disease
    MITCHELL TURKER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  4. Gene silencing in mammalian cells induced by environmental exposure
    MITCHELL TURKER; Fiscal Year: 2007
    ..Three specific aims are offered. For the first aim, expression of the selectable mouse Aprt (adenosine phosphoribosyl transferase) coding region will be controlled with the tet on/off system in cultured ..
  5. TEM with Digital and Cryopreparation Systems
    Andrew Evan; Fiscal Year: 2003
    ..Dr. Richard Gregory will employ immunohistochemistry to examine the role of a Streptococcus mutans 65 kDa fimbrial protein binding as a mechanism for the induction of dental carries. ..
  6. IKSI: 1st Annual International Urolithiasis Research Symposium
    Andrew Evan; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  7. Risk for Renal Injury Caused by Shock Wave Lithotripsy
    Andrew Evan; Fiscal Year: 2008
    ..All of these proposed studies aim at understanding the adverse effects of shock waves and advancing the safety of all stone patients at a time when reports of adverse effects in SWL are on the rise. ..
  8. IKSI: 2nd International Urolithiasis Research Symposium
    Andrew Evan; Fiscal Year: 2008
    ..This assumption was supported by the great success of the 1st International Urolithiasis Research symposium and the high level of interest in the 2nd symposium date. [unreadable] [unreadable] [unreadable]..