VDAC1

Summary

Gene Symbol: VDAC1
Description: voltage dependent anion channel 1
Alias: PORIN, VDAC-1, voltage-dependent anion-selective channel protein 1, outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, porin 31HM, sperm binding protein 1a
Species: human

Top Publications

  1. pmc Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
    M Narita
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:14681-6. 1998
  2. doi Voltage-dependent anion channel 1 is involved in endostatin-induced endothelial cell apoptosis
    Shaopeng Yuan
    Beijing Key Laboratory for Protein Therapeutics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    FASEB J 22:2809-20. 2008
  3. doi Human voltage-dependent anion selective channel 1 is a target antigen for antiglomerular endothelial cell antibody in mixed connective tissue disease
    Tamami Kikuchi
    Department of Cellular Physiology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Niigata, 951 8510, Japan
    Mod Rheumatol 18:570-7. 2008
  4. ncbi Downregulation of voltage-dependent anion channel-1 expression by RNA interference prevents cancer cell growth in vivo
    Inbar Koren
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cancer Biol Ther 9:1046-52. 2010
  5. pmc Isolation of the mitochondrial benzodiazepine receptor: association with the voltage-dependent anion channel and the adenine nucleotide carrier
    M W McEnery
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185
    Proc Natl Acad Sci U S A 89:3170-4. 1992
  6. ncbi [Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)]
    H Kayser
    Max Planck Institut fur experimentelle Medizin, Abteilung Immunchemie, Gottingen
    Biol Chem Hoppe Seyler 370:1265-78. 1989
  7. ncbi Cyclophilin-D binds strongly to complexes of the voltage-dependent anion channel and the adenine nucleotide translocase to form the permeability transition pore
    M Crompton
    Department of Biochemistry and Molecular Biology, University College London, UK
    Eur J Biochem 258:729-35. 1998
  8. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
  9. pmc Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, CREST of Japan Science and Technology Corporation JST, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:577-82. 2000
  10. pmc BH4 domain of antiapoptotic Bcl-2 family members closes voltage-dependent anion channel and inhibits apoptotic mitochondrial changes and cell death
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, and Core Research for Evolutional Science and Technology CREST of Japan Science and Technology Corporation, 2 2 Yamadaoka, Suita
    Proc Natl Acad Sci U S A 97:3100-5. 2000

Research Grants

  1. LEE MARK WETZLER; Fiscal Year: 2016
  2. Porins of Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2009
  3. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
  4. OUTER MEMBRANE PERMEABILITY OF PSEUDOMONAS CEPACIA
    Jane Burns; Fiscal Year: 1992
  5. HOST CELL RECOGNITION OF SALMONELLA TYPHIMURIUM
    THOMAS PISTOLE; Fiscal Year: 2003
  6. STRUCTURE-FUNCTION STUDIES OF MOLECULAR CHAPERONES
    David McKay; Fiscal Year: 2007
  7. IMMUNOPROTECTIVE POTENTIAL OF PORINS AND ANTI-PORIN MABS
    Shiva Singh; Fiscal Year: 1991
  8. What controls the thickness of biological membranes
    Jonathan Sachs; Fiscal Year: 2006
  9. EFFECT OF PORA ON MENINGOCOCCI-MACROPHAGE INTERACTION
    Lillian Waldbeser; Fiscal Year: 1999
  10. REGULATION OF PULMONARY CIRCULATION IN FETUS AND NEWBORN
    Girija G Konduri; Fiscal Year: 2013

Detail Information

Publications233 found, 100 shown here

  1. pmc Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
    M Narita
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:14681-6. 1998
    ..Taken together, these findings suggest that proapoptotic Bcl-2 family proteins, including Bax and Bak, induce the mitochondrial PT and cytochrome c release by interacting with the PT pores...
  2. doi Voltage-dependent anion channel 1 is involved in endostatin-induced endothelial cell apoptosis
    Shaopeng Yuan
    Beijing Key Laboratory for Protein Therapeutics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    FASEB J 22:2809-20. 2008
    ..ES promotes the mitochondrial permeability transition pore (mPTP) opening via voltage-dependent anion channel 1 (VDAC1), a major component of mitochondrial outer membrane...
  3. doi Human voltage-dependent anion selective channel 1 is a target antigen for antiglomerular endothelial cell antibody in mixed connective tissue disease
    Tamami Kikuchi
    Department of Cellular Physiology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Niigata, 951 8510, Japan
    Mod Rheumatol 18:570-7. 2008
    ....
  4. ncbi Downregulation of voltage-dependent anion channel-1 expression by RNA interference prevents cancer cell growth in vivo
    Inbar Koren
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cancer Biol Ther 9:1046-52. 2010
    The voltage-dependent anion channel 1 (VDAC1), located at the outer mitochondrial membrane (OMM), is a key protein in regulating the exchange of ions, nucleotides and a variety of metabolites in and out of the mitochondria...
  5. pmc Isolation of the mitochondrial benzodiazepine receptor: association with the voltage-dependent anion channel and the adenine nucleotide carrier
    M W McEnery
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185
    Proc Natl Acad Sci U S A 89:3170-4. 1992
    ..VDAC and ADC, outer and inner mitochondrial membrane channel proteins, respectively, together with the 18-kDa subunit, may comprise mBzR at functionally important transport sites at the junction of two mitochondrial membranes...
  6. ncbi [Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)]
    H Kayser
    Max Planck Institut fur experimentelle Medizin, Abteilung Immunchemie, Gottingen
    Biol Chem Hoppe Seyler 370:1265-78. 1989
    ..structure of a human porin with the molecular mass of 31 kDa derived from the plasmalemm of B-lymphocytes (Porin 31HL). Porin 31HL is shown to be a basic, channel forming membrane protein...
  7. ncbi Cyclophilin-D binds strongly to complexes of the voltage-dependent anion channel and the adenine nucleotide translocase to form the permeability transition pore
    M Crompton
    Department of Biochemistry and Molecular Biology, University College London, UK
    Eur J Biochem 258:729-35. 1998
    ..band on SDS/PAGE resolved into a doublet and reacted with antibodies against the voltage-dependent anion channel (porin) and the adenine nucleotide translocase...
  8. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
    ..In agreement with this, VDAC1-deficient mitochondria from a mutant yeast did not exhibit a Bax/Bak-induced loss in membrane potential and ..
  9. pmc Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, CREST of Japan Science and Technology Corporation JST, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:577-82. 2000
    ..These findings indicated the involvement of a different mechanism in BH3-only, protein-induced apoptogenic cytochrome c release...
  10. pmc BH4 domain of antiapoptotic Bcl-2 family members closes voltage-dependent anion channel and inhibits apoptotic mitochondrial changes and cell death
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, and Core Research for Evolutional Science and Technology CREST of Japan Science and Technology Corporation, 2 2 Yamadaoka, Suita
    Proc Natl Acad Sci U S A 97:3100-5. 2000
    ..Finally, the data suggest that the TAT-BH4 peptide is potentially useful as a therapeutic agent for diseases caused by accelerated apoptosis...
  11. pmc Control of mitochondrial membrane permeabilization by adenine nucleotide translocator interacting with HIV-1 viral protein rR and Bcl-2
    E Jacotot
    Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, F 94805 Villejuif, France
    J Exp Med 193:509-19. 2001
    ..Concomitantly, Bcl-2 suppresses channel formation by the ANT-Vpr complex in synthetic membranes. In conclusion, both Vpr and Bcl-2 modulate MMP through a direct interaction with ANT...
  12. ncbi Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74
    Christian Schwarzer
    Max Planck Institute for Experimental Medicine, Department of Immunochemistry, Hermann Rein Street 3, 37075 Gottingen, Germany
    Int J Biochem Cell Biol 34:1059-70. 2002
    The voltage-dependent anion-selective channel 1 (VDAC1), i.e...
  13. ncbi Identification of the protein-protein contact site and interaction mode of human VDAC1 with Bcl-2 family proteins
    Yong Shi
    The Center for Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
    Biochem Biophys Res Commun 305:989-96. 2003
    ..both pro-apoptotic Bax and anti-apoptotic Bcl-X(L) might simultaneously contact the putative loop regions of human VDAC1, and the existence of VDAC1-Bax-Bcl-X(L) tertiary complex in vitro suggested that VDAC1 channel conformation and ..
  14. ncbi Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells
    Changjiang Weng
    Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China 100080
    J Biol Chem 280:10491-500. 2005
    ..These results suggested that removal of N-terminal domains of Bid by caspase-8 and Mcl-1 by caspase-3 enabled the maximal mitochondrial perturbation that potentiated TRAIL-induced apoptosis...
  15. pmc NMR structural investigation of the mitochondrial outer membrane protein VDAC and its interaction with antiapoptotic Bcl-xL
    Thomas J Malia
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 46:514-25. 2007
    ..Our characterization of the VDAC/Bcl-xL complex offers initial structural insight into the role of antiapoptotic Bcl-xL in regulating apoptotic events in the mitochondrial outer membrane...
  16. pmc Solution structure of the integral human membrane protein VDAC-1 in detergent micelles
    Sebastian Hiller
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Science 321:1206-10. 2008
    ..NMR measurements revealed the binding sites of VDAC-1 for the Bcl-2 protein Bcl-x(L), for reduced beta-nicotinamide adenine dinucleotide, and for cholesterol. Bcl-x(L) interacts with the VDAC barrel laterally at strands 17 and 18...
  17. pmc Structure of the human voltage-dependent anion channel
    Monika Bayrhuber
    Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 105:15370-5. 2008
    The voltage-dependent anion channel (VDAC), also known as mitochondrial porin, is the most abundant protein in the mitochondrial outer membrane (MOM)...
  18. doi Voltage-dependent anion channel 1-based peptides interact with hexokinase to prevent its anti-apoptotic activity
    Laetitia Arzoine
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel 84105
    J Biol Chem 284:3946-55. 2009
    ..Now, we demonstrate that synthetic peptides corresponding to the VDAC1 N-terminal region and selected sequences bound specifically, in a concentration- and time-dependent manner, to ..
  19. doi Outer membrane VDAC1 controls permeability transition of the inner mitochondrial membrane in cellulo during stress-induced apoptosis
    Flora Tomasello
    INSERM U916, Universite Bordeaux 2, Institut Bergonie, 33076 Bordeaux, France
    Cell Res 19:1363-76. 2009
    ..Working at the single live cell level, we found that overexpression of VDAC1 triggers MPT at the mitochondrial inner membrane (MIM)...
  20. doi PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1
    Sven Geisler
    Laboratory of Functional Neurogenetics, Otfried Muller Strasse 27, 72076 Tubingen, Germany
    Nat Cell Biol 12:119-31. 2010
    ..Strikingly, we identified VDAC1 (voltage-dependent anion channel 1) as a target for Parkin-mediated Lys 27 poly-ubiquitylation and mitophagy...
  21. doi Swapping of the N-terminus of VDAC1 with VDAC3 restores full activity of the channel and confers anti-aging features to the cell
    Simona Reina
    Department of Chemical Sciences, University of Catania, Catania, Italy
    FEBS Lett 584:2837-44. 2010
    ..The substitution of the VDAC3 N-terminus with the VDAC1 N-terminus caused the chimaera to become more active than VDAC1...
  22. pmc VDAC1 selectively transfers apoptotic Ca2+ signals to mitochondria
    D De Stefani
    Department of Biomedical Sciences, University of Padova and CNR Neuroscience Institute, Padua, Italy
    Cell Death Differ 19:267-73. 2012
    ..We show that VDAC1 silencing potentiates apoptotic challenges, whereas VDAC2 has the opposite effect...
  23. doi Structure-based analysis of VDAC1: N-terminus location, translocation, channel gating and association with anti-apoptotic proteins
    Shay Geula
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biochem J 444:475-85. 2012
    Structural studies place the VDAC1 (voltage-dependent anion channel 1) N-terminal region within the channel pore. Biochemical and functional studies, however, reveal that the N-terminal domain is cytoplasmically exposed...
  24. pmc Mediation of the antiapoptotic activity of Bcl-xL protein upon interaction with VDAC1 protein
    Nir Arbel
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 287:23152-61. 2012
    ..We previously demonstrated the direct interaction of Bcl2 with VDAC, leading to reduced channel conductance. VDAC1-based peptides interacted with Bcl2 to prevent its antiapoptotic activity...
  25. pmc Voltage-dependent anion channels (VDACs) recruit Parkin to defective mitochondria to promote mitochondrial autophagy
    Yu Sun
    Department of Neurobiology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA
    J Biol Chem 287:40652-60. 2012
    ..Parkin is recruited to defective mitochondria to promote degradation by an autophagy mechanism (mitophagy)...
  26. ncbi Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel
    E Blachly-Dyson
    Vollum Institute for Advanced Biomedical Research, Portland, Oregon
    J Biol Chem 268:1835-41. 1993
    ..The expression of each human cDNA has been assessed by Northern blot and polymerase chain reaction techniques. With one exception, each is expressed in all human cell lines and tissues examined...
  27. pmc The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore
    E Jacotot
    Centre National de la Recherche Scientifique, F 94801 Villejuif, France
    J Exp Med 191:33-46. 2000
    ..Hence, Vpr induces apoptosis via a direct effect on the mitochondrial PTPC...
  28. doi Hexokinase-I protection against apoptotic cell death is mediated via interaction with the voltage-dependent anion channel-1: mapping the site of binding
    Salah Abu-Hamad
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 283:13482-90. 2008
    ..We have previously shown that HK-I decreases murine VDAC1 (mVDAC1) channel conductance, inhibits cytochrome c release, and protects against apoptotic cell death...
  29. pmc Paraquat toxicity induced by voltage-dependent anion channel 1 acts as an NADH-dependent oxidoreductase
    Hiroki Shimada
    Molecular and Cell Structural Science, Kanazawa Medical University, Uchinada, Ishikawa 920 0293, Japan
    J Biol Chem 284:28642-9. 2009
    ..we demonstrated that mitochondrial NADH-dependent PQ reductase containing a voltage-dependent anion channel 1 (VDAC1) is responsible for PQ cytotoxicity...
  30. pmc Neisseria meningitidis porin PorB interacts with mitochondria and protects cells from apoptosis
    P Massari
    Evans Biomedical Research Center, 650 Albany Street, Boston University School of Medicine, Boston Medical Center, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 97:9070-5. 2000
    Neisserial porins are strong immune adjuvants and B cell activators. The effect of neisserial porin PorB on activation-induced cell death was investigated, as a potential additional mechanism of the porin's immunopotentiating ability...
  31. ncbi Functional characterization of the conserved "GLK" motif in mitochondrial porin from Neurospora crassa
    G Runke
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    J Bioenerg Biomembr 32:563-70. 2000
    Mitochondrial porin facilitates the diffusion of small hydrophilic molecules across the mitochondrial outer membrane...
  32. ncbi [The mechanism of antigen-presentation of porin from Salmonella typhimurium to T lymphocytes]
    K Matsui
    Department of Microbiology, Meiji College of Pharmacy
    Kansenshogaku Zasshi 68:491-4. 1994
    ..It is understood that these three species of proteins construct porin trimers and the porin trimers from the wild type strain of S...
  33. ncbi Modulation of the voltage-dependent anion channel (VDAC) by glutamate
    D Gincel
    Department of Life Sciences, The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel
    J Bioenerg Biomembr 32:571-83. 2000
    The voltage-dependent anion channel (VDAC), also known as mitochondrial porin, is a large channel permeable to anions, cations, ATP, and other metabolites...
  34. ncbi Molecular and cellular mechanisms used in the acute phase of stimulated steroidogenesis
    M Thomson
    Department of Biological Sciences, University of Western Sydney Nepean, Kingswood, Australia
    Horm Metab Res 30:16-28. 1998
    ..These contact sites may be composed of protein bridges which include the constituents, porin, the benzodiazepine receptor and GTP binding proteins...
  35. ncbi [Alteration of serotype and drug susceptibility of some Pseudomonas aeruginosa isolates by exposure to human serum and polymorphonuclear leukocytes]
    I Kobayashi
    Chemotherapy Division, Mitsubishi Yuka Bio Clinical Laboratories, Inc. Tokyo
    Kansenshogaku Zasshi 68:500-7. 1994
    ..susceptibilities to some beta-lactams and norfloxacin which could penetrate into the bacterial cells through the porin channels of the outer membrane...
  36. ncbi Binding of rat brain hexokinase to recombinant yeast mitochondria: identification of necessary molecular determinants
    H Azoulay-Zohar
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    J Bioenerg Biomembr 31:569-79. 1999
    ..brain hexokinase to mitochondria from rat liver or yeast (wild type, porinless, or expressing recombinant human porin) was studied in an effort to identify minimal requirements for each component...
  37. ncbi Diffusion of beta-lactam antibiotics through oligomeric or monomeric porin channels of some gram-negative bacteria
    Yeshayahu Nitzan
    Health Sciences Research Center, Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Curr Microbiol 45:446-55. 2002
    ..The major proteins with porin activity were purified from the outer membranes of six bacteria. Three of the six porins were oligomeric porins...
  38. ncbi Homology models of the Yersinia pseudotuberculosis and Yersinia pestis general porins and comparative analysis of their functional and antigenic regions
    G N Likhatskaya
    Pacific Institute of Bioorganic Chemistry of Far East Branch of Russian Academy of Sciences, Vladivostok, Pr 100 Let Vladivostoku 159, 690022 Russia
    J Biomol Struct Dyn 23:163-74. 2005
    The amino acid sequences of the Yersinia pseudotuberculosis porin (YPS) and Y. pestis porin (YPT) have recently deduced but their three-dimensional structures were not known...
  39. pmc Energy-dependent accumulation of norfloxacin and porin expression in clinical isolates of Klebsiella pneumoniae and relationship to extended-spectrum beta-lactamase production
    Luis Martinez-Martinez
    Department of Microbiology, School of Medicine, University of Seville, Spain
    Antimicrob Agents Chemother 46:3926-32. 2002
    The relationships between porin deficiency, active efflux of fluoroquinolones, and extended-spectrum beta-lactamase (ESBL) production were determined for 53 clinical isolates of Klebsiella pneumoniae...
  40. ncbi Characterization of further association of the trimeric membrane protein porin by low-angle laser-light scattering photometry coupled with high-performance gel chromatography
    Y Watanabe
    Institute for Protein Research, Osaka University, Japan
    J Chromatogr A 653:241-6. 1993
    b>Porin (OmpF), a trimeric membrane protein, in an extract of the outer membrane of Escherichia coli gave a twin-peaked elution pattern on Sephacryl S-300HR gel chromatography in the presence of sodium dodecyl sulphate...
  41. pmc X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: implications for ion selectivity and single-channel conductance
    M Przybylski
    Universitat Konstanz, Fakultat fur Chemie, Germany
    Protein Sci 5:1477-89. 1996
    ..To introduce new negative charges in a channel protein, amino groups of porin from Rhodobacter capsulatus 37b4 were succinylated with succinic anhydride, and the precise extent and sites of ..
  42. ncbi Identification of positively charged residues of FomA porin of Fusobacterium nucleatum which are important for pore function
    H Kleivdal
    Department Molecular Biology, Unviversity of Bergen, Norway
    Eur J Biochem 260:818-24. 1999
    FomA porin is the major outer-membrane protein of Fusobacterium nucleatum. It exhibits the functional properties of a general diffusion porin, but has no sequence similarity to other porins...
  43. pmc Porins from Salmonella enterica serovar Typhimurium activate the transcription factors activating protein 1 and NF-kappaB through the Raf-1-mitogen-activated protein kinase cascade
    Massimiliano Galdiero
    Istituto di Microbiologia, Facolta di Medicina e Chirurgia, Seconda Universita degli Studi di Napoli, 80138 Naples, Italy
    Infect Immun 70:558-68. 2002
    ..that these enzymes may participate in cell signaling pathways leading to AP-1 and NF-kappaB activation following porin stimulation of cells...
  44. pmc Molecular, antigenic, and functional analyses of Omp2b porin size variants of Brucella spp
    J Y Paquet
    Unité de Recherche en Biologie Moléculaire URBM, Laboratoire d Immunologie Microbiologie, Facultes Universitaires Notre Dame de la Paix, 5000 Namur, Belgium
    J Bacteriol 183:4839-47. 2001
    ..The variation of the Omp2a and Omp2b porin sequences among the various strains of the genus Brucella seems to result mostly from multiple gene conversions ..
  45. pmc Roles of beta-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniae
    L Martinez-Martinez
    Department of Microbiology, School of Medicine, University of Seville, Seville, Spain
    Antimicrob Agents Chemother 43:1669-73. 1999
    ..The contribution of porin deficiency was investigated by restoring a functional ompK36 gene on a plasmid...
  46. pmc How beta-lactam antibiotics enter bacteria: a dialogue with the porins
    Chloe E James
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Faculte de Medecine, IFR 88, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e5453. 2009
    ..Clinical reports have linked the MDR phenotype to altered membrane permeability including porin modification and efflux pump expression.
  47. doi A putative alpha-helical porin from Corynebacterium glutamicum
    Karin Ziegler
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstr 21, 79104 Freiburg im Breisgau, Germany
    J Mol Biol 379:482-91. 2008
    ..The exchange of material across this barrier requires porins. Porin B (PorB) is one of them...
  48. pmc Role of porins for uptake of antibiotics by Mycobacterium smegmatis
    Olga Danilchanka
    Department of Microbiology, University of Alabama at Birmingham, Alabama 35294, USA
    Antimicrob Agents Chemother 52:3127-34. 2008
    ..Here, we examined which antibiotics utilize the porin pathway across the outer membrane of the model organism Mycobacterium smegmatis...
  49. ncbi The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion
    I Poquet
    , Institut Pasteur, Paris, France
    Mol Microbiol 9:1061-9. 1993
    ..pullulanase variant with a serine at position +2 cofractionated mainly with the major peak of outer membrane porin. In contrast, most (55%) of a pullulanase variant with an aspartate at position +2 cofractionated with slightly ..
  50. ncbi The gene bglH present in the bgl operon of Escherichia coli, responsible for uptake and fermentation of beta-glucosides encodes for a carbohydrate-specific outer membrane porin
    C Andersen
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Germany
    Mol Microbiol 31:499-510. 1999
    ..The resulting plasmid was transferred into the porin-deficient E. coli strain KS26 and the protein was expressed by addition of IPTG...
  51. doi Paradoxical effect of Klebsiella pneumoniae OmpK36 porin deficiency
    Xiuhong Jiang
    Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, NSW, Australia
    Pathology 41:388-92. 2009
    ..To evaluate the effect of defined mutations in the major OmpK35 and OmpK36 porins in Klebsiella pneumoniae on the activity of two common plasmid-mediated AmpC enzymes...
  52. pmc Excretion of endogenous cadaverine leads to a decrease in porin-mediated outer membrane permeability
    H Samartzidou
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5513, USA
    J Bacteriol 181:791-8. 1999
    The permeability of the outer membrane of Escherichia coli to hydrophilic compounds is controlled by porin channels...
  53. ncbi Topological investigations of the FomA porin from Fusobacterium nucleatum and identification of the constriction loop L6
    H Kleivdal
    Department of Molecular Biology, University of Bergen, HIB, Thormøhlensgate 55, N 5020 Bergen, Norway
    Microbiology 147:1059-67. 2001
    b>Porin FomA in the outer membrane of Fusobacterium nucleatum is a trimeric protein, which exhibits permeability properties similar to that of the well-known enterobacterial diffusion porins...
  54. ncbi Induction of tyrosine phosphorylated proteins in THP-1 cells by Salmonella typhimurium, Pasteurella haemolytica and Haemophilus influenzae porins
    M Galdiero
    Dipartimento di Patologia e Sanita Animale, Facolta di Veterinaria, Universita degli Studi di Napoli Federico II, Naples, Italy
    FEMS Immunol Med Microbiol 31:121-30. 2001
    ..After porin stimulation a pattern of tyrosine phosphorylated proteins appeared in the soluble cytoplasmic fraction, in the ..
  55. ncbi Site-directed mutagenesis studies to probe the role of specific residues in the external loop (L3) of OmpF and OmpC porins in susceptibility of Serratia marcescens to antibiotics
    Sanela Begic
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Can J Microbiol 53:710-9. 2007
    ..to this natural antibiotic resistance is reduced outer membrane permeability, controlled in part by OmpF and OmpC porin proteins...
  56. pmc The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant
    Xiuping Liu
    Evans Biomedical Research Center, Section of Infectious Diseases, Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    Vaccine 26:786-96. 2008
    ..The PorB porin from Neisseria lactamica (Nlac), a common nasopharyngeal commensal, shares significant structural and functional ..
  57. ncbi A novel OmpY porin from Yersinia pseudotuberculosis: structure, channel-forming activity and trimer thermal stability
    T F Solov'eva
    Pacific Institute of Bioorganic Chemistry FEBRAS, Prospect 100 Let Vladivostoku 159, Vladivostok 690022, Russia
    J Biomol Struct Dyn 28:517-33. 2011
    A novel OmpY porin was predicted based on the Yersinia pseudotuberculosis genome analysis. Whereas it has the different genomic annotation such as "outer membrane protein N" (ABS46310.1) in str...
  58. pmc Putative porin of Bradyrhizobium sp. (Lupinus) bacteroids induced by glyphosate
    Nuria de María
    Instituto de Recursos Naturales, Centro de Ciencias Medioambientales, CSIC, Serrano, 115 bis, E 28006 Madrid, Spain
    Appl Environ Microbiol 73:5075-82. 2007
    ..It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a ..
  59. ncbi Molecular characterization of the interaction between porins of Neisseria gonorrhoeae and C4b-binding protein
    Hanna Jarva
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, University of Lund, Malmo, Sweden
    J Immunol 179:540-7. 2007
    ..The resistance of N. gonorrhoeae to normal human serum killing correlates with porin (Por)-mediated binding to the complement inhibitor, C4b-binding protein (C4BP)...
  60. pmc Expression and porin activity of P28 and OMP-1F during intracellular Ehrlichia chaffeensis development
    Yumi Kumagai
    Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210 1093, USA
    J Bacteriol 190:3597-605. 2008
    ..Here we demonstrate that isolated E. chaffeensis outer membranes have porin activities, as determined by a proteoliposome swelling assay...
  61. doi The influence of sterols on the conformation of recombinant mitochondrial porin in detergent
    Denice C Bay
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Biochem Cell Biol 86:539-45. 2008
    ..Nonetheless, the contributions of sterols to the folded state of mitochondrial porin are not understood...
  62. ncbi Functional characterization of a second porin isoform in Drosophila melanogaster. DmPorin2 forms voltage-independent cation-selective pores
    Rita Aiello
    Department of Chemical Sciences, University of Catania, 95125 Catania, Italy
    J Biol Chem 279:25364-73. 2004
    ..Genome sequencing of the fruit fly Drosophila melanogaster revealed the presence of three additional porin-like genes. No functional information was available for the different gene products...
  63. ncbi VDAC1 serves as a mitochondrial binding site for hexokinase in oxidative muscles
    Keltoum Anflous-Pharayra
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Biochim Biophys Acta 1767:136-42. 2007
    ..that mitochondria-bound hexokinase activity is significantly reduced in oxidative muscles (heart and soleus) in vdac1(-/-) mice. The activity data were supported by western blot analysis using HK2 specific antibody...
  64. pmc Moraxella catarrhalis M35 is a general porin that is important for growth under nutrient-limiting conditions and in the nasopharynges of mice
    Donna M Easton
    Faculty of Science, University of Canberra, Canberra, Australian Capital Territory 2601, Australia
    J Bacteriol 190:7994-8002. 2008
    ..We have previously predicted the outer membrane protein M35 to be a general porin, and in the current study, we have investigated the function of M35 and its importance for survival of M...
  65. ncbi Resistance to extended-spectrum beta-lactams by the emergence of SHV-12 and the loss of OmpK35 in Klebsiella pneumoniae and Escherichia coli in Malaysia
    Selvi Palasubramaniam
    Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia
    J Microbiol Immunol Infect 42:129-33. 2009
    ..This study describes the detection of SHV-12 extended-spectrum beta-lactamase (ESBL) subtype and the loss of OmpK35 porin in 4 strains of K. pneumoniae and E. coli.
  66. ncbi Porin isolated from the outer membrane of Erwinia amylovora and its encoding gene
    M Elazar
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
    Curr Microbiol 54:155-61. 2007
    ..The monomers of Omp-EA porin presented a topology of 16 transmembranal beta-strands...
  67. ncbi Distinct sensitivities of OmpF and PhoE porins to charged modulators
    H Samartzidou
    Department of Biology and Biochemistry, University of Houston, TX 77204 5513, USA
    FEBS Lett 444:65-70. 1999
    The inhibition of the anion-selective PhoE porin by ATP and of the cation-selective OmpF porin by polyamines has been previously documented...
  68. ncbi Identification of porin-like polypeptide(s) in the boundary membrane of oilseed glyoxysomes
    F J Corpas
    Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estacion Experimental del Zaidin, CSIC, Apdo 419, E 18080 Granada, Spain
    Plant Cell Physiol 41:1218-28. 2000
    ..Evidence is presented in this study that this 36-kDa polypeptide is a glyoxysomal membrane porin. A sequence of 24 amino acid residues derived from a CNBr-cleaved fragment of the 36-kDa polypeptide revealed 72% ..
  69. doi The role of lipids for the functional integrity of porin: an FTIR study using lipid and protein reporter groups
    Filiz Korkmaz
    Institute of Biophysics, Johann Wolfgang Goethe University, Max von Laue Strasse 1, D 60438 Frankfurt am Main, Germany
    Biochemistry 47:12126-34. 2008
    ..girdle of OmpF and the interfacial region of the lipid bilayer, leading to monomerization of this trimeric porin. The second step at 75 degrees C is attributed to the changes in lipid-porin monomer interaction...
  70. pmc Polyamines decrease Escherichia coli outer membrane permeability
    A L Dela Vega
    Department of Biology, University of Houston, Texas 77204, USA
    J Bacteriol 178:3715-21. 1996
    ..of the outer membranes of gram-negative bacteria to hydrophilic compounds is mostly due to the presence of porin channels...
  71. ncbi Crystal structure of Omp32, the anion-selective porin from Comamonas acidovorans, in complex with a periplasmic peptide at 2.1 A resolution
    K Zeth
    Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Structure 8:981-92. 2000
    ..Here, we investigate the strongly anion-selective porin Omp32 from Comamonas acidovorans, which is closely homologous to the porins of pathogenic Bordetella and Neisseria ..
  72. ncbi The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients
    Joachim Stephan
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, D 91058 Erlangen, Germany
    Mol Microbiol 58:714-30. 2005
    ..Nutrients cross this permeability barrier by diffusion through porins. MspA is the major porin of Mycobacterium smegmatis...
  73. pmc Porin protein of Neisseria gonorrhoeae: cloning and gene structure
    E C Gotschlich
    Laboratory of Bacteriology and Immunology, Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 84:8135-9. 1987
    The outer membrane porin molecule of Neisseria gonorrhoeae is known as protein I (PI). Among different strains of gonococci there is variability of PI, and two main classes, PIA and PIB, have been recognized...
  74. ncbi Effects of a mutation in the Drosophila porin gene encoding mitochondrial voltage-dependent anion channel protein on phototransduction
    Sunji Lee
    Graduate School of Biotechnology, Kyunghee University, Yongin si, Gyeonggi Do, Korea
    Dev Neurobiol 67:1533-45. 2007
    ..So far three distinct isoforms of VDAC (VDAC1-3) have been reported in vertebrates, but their functions remain unknown...
  75. pmc Downregulation of mitochondrial porin inhibits cell growth and alters respiratory phenotype in Trypanosoma brucei
    Ujjal K Singha
    Department of Microbial Pathogenesis and Immune Response, Meharry Medical College, Nashville, TN 37208, USA
    Eukaryot Cell 8:1418-28. 2009
    b>Porin is the most abundant outer membrane (OM) protein of mitochondria. It forms the aqueous channel on the mitochondrial OM and mediates major metabolite flux between mitochondria and cytosol...
  76. pmc Outer membrane porin M35 of Moraxella catarrhalis mediates susceptibility to aminopenicillins
    Marion Jetter
    Institute for Infectious Diseases, University of Bern, CH 3010 Bern, Switzerland
    BMC Microbiol 9:188. 2009
    The outer membrane protein M35 is a conserved porin of type 1 strains of the respiratory pathogen Moraxella catarrhalis...
  77. ncbi Structural architecture of an outer membrane channel as determined by electron crystallography
    B K Jap
    Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Nature 350:167-70. 1991
    ..b>Porin channels have been shown in vitro to be voltage-gated...
  78. ncbi Tuning of the porin expression under anaerobic growth conditions by his-to-Asp cross-phosphorelay through both the EnvZ-osmosensor and ArcB-anaerosensor in Escherichia coli
    M Matsubara
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Genes Cells 5:555-69. 2000
    ..We have addressed this issue, with special reference to the osmo-responsive EnvZ and anaero-responsive ArcB phosphorelay signalling pathways in E. coli...
  79. pmc Cadaverine inhibition of porin plays a role in cell survival at acidic pH
    Hrissi Samartzidou
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA
    J Bacteriol 185:13-9. 2003
    When grown at acidic pH, Escherichia coli cells secrete cadaverine, a polyamine known to inhibit porin-mediated outer membrane permeability...
  80. doi Altered pore properties and kinetic changes in mutants of the Vibrio cholerae porin OmpU
    Brian Lauman
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5001, USA
    Mol Membr Biol 25:498-505. 2008
    ..was used to characterize the pore properties of site-directed mutants in the Vibrio cholerae general diffusion porin OmpU...
  81. pmc Characterisation of porin genes from Mycobacterium fortuitum and their impact on growth
    Soroush Sharbati
    Freie Universitat Berlin, Institute of Veterinary Biochemistry, Berlin, Germany
    BMC Microbiol 9:31. 2009
    ..Here, porin genes from M...
  82. ncbi Crystal structure of the monomeric porin OmpG
    Gowtham V Subbarao
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    J Mol Biol 360:750-9. 2006
    ..In contrast, the OmpG porin is unique, as it appears to function as a monomer...
  83. pmc Phenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU
    Melissa Pagel
    Department of Biology and Biochemistry, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
    J Bacteriol 189:8593-600. 2007
    ..Here, we have analyzed the effects of mutations in the OmpU porin of Vibrio cholerae at conserved residues that are known to affect pore properties in the Escherichia coli porins ..
  84. pmc The porin OmpD from nontyphoidal Salmonella is a key target for a protective B1b cell antibody response
    Cristina Gil-Cruz
    School of Immunity and Infection and Medical Research Council Centre for Immune Regulation, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Proc Natl Acad Sci U S A 106:9803-8. 2009
    ..Furthermore Ab from porin-immunized chimeras, that have B1b cells, is sufficient to impair infection...
  85. doi Proteomic analysis of human U937 cell line activation mediated by Haemophilus influenzae type b P2 porin and its surface-exposed loop 7
    Valeria Severino
    Department of Life Science, Second University of Naples, Via Vivaldi 43, I 81100 Caserta, Italy
    J Proteome Res 9:1050-62. 2010
    ..P2 porin, one of the best-characterized porins in terms of its functional characteristics, is the most abundant OMP in Hib ..
  86. ncbi A common channel-forming motif in evolutionarily distant porins
    R A Pauptit
    Biocenter, University of Basel, Switzerland
    J Struct Biol 107:136-45. 1991
    ..packings of Escherichia coli porins are presented (phosphoporin, maltoporin, and two crystal forms of matrix porin)...
  87. ncbi The quantitation of ADP diffusion gradients across the outer membrane of heart mitochondria in the presence of macromolecules
    Frank Norbert Gellerich
    Muskellabor der Neurologischen Klinik und Poliklinik, Martin Luther Universitat, Halle Wittenberg, Julius Kühn Str 7, D 06097 Halle Saale, Halle Wittenberg, Germany
    Biochim Biophys Acta 1554:48-56. 2002
    We have previously provided evidence that diffusion of metabolites across the porin pores of mitochondrial outer membrane is hindered...
  88. pmc DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus
    T A Ficht
    Department of Veterinary Microbiology and Parasitology, Texas A and M, University, College Station 77843
    Infect Immun 57:3281-91. 1989
    ..The amino acid composition of the predicted proteins was similar to that obtained for the Brucella porin isolated by Verstreate et al. (D. R. Verstreate, M. T. Creasy, N. T. Caveney, C. L. Baldwin, M. W. Blab, and A. J...
  89. ncbi Crystal structure and functional characterization of OmpK36, the osmoporin of Klebsiella pneumoniae
    R Dutzler
    Department of Structural Biology, Biozentrum University of Basel, CH 4056 Basel, Switzerland
    Structure 7:425-34. 1999
    ..In Escherichia coli, major nonspecific porins are matrix porin (OmpF) and osmoporin (OmpC), which show high sequence homology...
  90. ncbi The porin protein of the outer membrane of Escherichia coli: reactivity in immunoblotting, antibody-binding by the native protein, and cross-reactivity with other enteric bacteria
    A Z Henriksen
    Department of Microbiology, Faculty of Medicine, University of Trondheim, Norway
    Acta Pathol Microbiol Immunol Scand B 95:315-21. 1987
    The experimental conditions for antibody-binding by the 38.5 kD porin protein of an E. coli 055 strain in immunoblotting were investigated...
  91. pmc Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli
    C Prigent-Combaret
    Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, CNRS UMR 5577, Institut National des Sciences Appliquees de Lyon, 69621 Villeurbanne, France
    J Bacteriol 181:5993-6002. 1999
    ..Different cell functions were more expressed in sessile bacteria: the OmpC porin, the high-affinity transport system of glycine betaine (encoded by the proU operon), the colanic acid ..
  92. ncbi VDAC1, having a shorter N-terminus than VDAC2 but showing the same migration in an SDS-polyacrylamide gel, is the predominant form expressed in mitochondria of various tissues
    Takenori Yamamoto
    Institute for Genome Research, University of Tokushima, Kuramotocho 3, Tokushima 770 8503, Japan
    J Proteome Res 5:3336-44. 2006
    ..Three isoforms of it, i.e., VDAC1, VDAC2, and VDAC3, are known to be expressed in mammals; however, the question as to which is the main isoform in ..
  93. doi Effects of ergosterol on the structure and activity of Neurospora mitochondrial porin in liposomes
    Denice C Bay
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Can J Microbiol 55:1275-83. 2009
    ..Therefore, in this work, a freeze-thaw technique was used to introduce recombinant Neurospora porin into liposomes containing, or lacking, the native fungal sterol ergosterol...
  94. doi Dynamic proteomic profile of potato tuber during its in vitro development
    Jae Woong Yu
    Department of Molecular Biotechnology, Konkuk University, 1, Hwayang dong, Gwangjin gu, Seoul 143 701, Republic of Korea
    Plant Sci 195:1-9. 2012
    ..Novel stage-specific proteins identified during in vitro tuberization were ferredoxin-NADP reductase, 34 kDa porin, aquaporin, calmodulin, ripening-regulated protein, and starch synthase...
  95. pmc The Acinetobacter baumannii Omp33-36 porin is a virulence factor that induces apoptosis and modulates autophagy in human cells
    Carlos Rumbo
    Department of Microbiology, Complejo Hospitalario Universitario A Coruña INIBIC, La Coruna, Spain
    Infect Immun 82:4666-80. 2014
    ..In the present study, we demonstrate that the A. baumannii Omp 33- to 36-kDa protein (Omp33-36) is a porin that acts as a channel for the passage of water...
  96. ncbi Porins and specific channels of bacterial outer membranes
    H Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Mol Microbiol 6:435-42. 1992
    ..The channels often have diameters in the range of 1 nm, and thus the penetration rates of solutes through porin channels are likely to be affected strongly by what appear to be minor differences in the size, shape, ..
  97. pmc Cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from Bordetella avium and expression of the gene in Salmonella typhimurium
    C R Gentry-Weeks
    Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland 20892
    J Bacteriol 174:7729-42. 1992
    ..to Neisseria gonorrhoeae outer membrane protein III, Haemophilus influenzae protein P6, and Pseudomonas aeruginosa porin protein F. The gene (ompA) encoding the B. avium 21-kDa protein hybridized with 4...
  98. ncbi Formation of active heterologous nitrate reductases between nitrate reductases A and Z of Escherichia coli
    F Blasco
    Laboratoire de Chimie Bacterienne, CNRS, Marseille, France
    Mol Microbiol 6:209-19. 1992
    ..The possibility that heterologous nitrate reductases could be formed in vivo is discussed with reference to the existence of porin heterotrimers of the outer membrane proteins OmpC, OmpF and PhoE.
  99. ncbi The bacterial Kdp K(+)-ATPase and its relation to other transport ATPases, such as the Na+/K(+)- and Ca2(+)-ATPases in higher organisms
    W Epstein
    Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637
    Philos Trans R Soc Lond B Biol Sci 326:479-86; discussion 486-7. 1990
    ..protein of other members of a family of pairs of regulatory proteins implicated in control of a variety of bacterial processes such as porin synthesis, phosphate regulon expression, nitrogen metabolism, chemotaxis and nodule formation.
  100. ncbi Deficiency of the human mitochondrial transcription factor h-mtTFA in infantile mitochondrial myopathy is associated with mtDNA depletion
    J Poulton
    Department of Paediatrics, University of Oxford, John Radcliffe Hospital, Headington, UK
    Hum Mol Genet 3:1763-9. 1994
    ..h-mtTFA was compared with other mitochondrial proteins such as pyruvate dehydrogenase and porin by Western blotting...
  101. ncbi Biophysical characterization of OprB, a glucose-inducible porin of Pseudomonas aeruginosa
    J L Wylie
    Department of Microbiology, University of Manitoba, Winnipeg, Canada
    J Bioenerg Biomembr 25:547-56. 1993
    OprB, a glucose-inducible porin of P. aeruginosa, was characterized by black lipid bilayer analysis and circular dichroism spectroscopy...

Research Grants63

  1. LEE MARK WETZLER; Fiscal Year: 2016
    DESCRIPTION (provided by applicant): Our laboratory has been investigating the immunobiology of the Neisserial porin PorB for the last two decades...
  2. Porins of Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2009
    ..We recently discovered two porins of M. tuberculosis that complement the permeability defects of a porin mutant of M. smegmatis. Both purified recombinant proteins showed channel activity in lipid bilayer experiments...
  3. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
    ..A high resolution (less than 2.9 angstroms) X-ray structure determination of the OMpF porin from E. coli is a major focus of the research effort...
  4. OUTER MEMBRANE PERMEABILITY OF PSEUDOMONAS CEPACIA
    Jane Burns; Fiscal Year: 1992
    ..A clinical isolate with an antibiotic-impermeable porin has been identified and the DNA encoding resistance has been cloned; the porin protein shows no apparent ..
  5. HOST CELL RECOGNITION OF SALMONELLA TYPHIMURIUM
    THOMAS PISTOLE; Fiscal Year: 2003
    ..b>Porin-deficient mutants will be compared with their corresponding wildtype counterparts in their ability to adhere to ..
  6. STRUCTURE-FUNCTION STUDIES OF MOLECULAR CHAPERONES
    David McKay; Fiscal Year: 2007
    ..vitro peptide binding activity with in vivo interactions with membrane proteins, and ultimately to develop an in vitro assay of bacterial porin folding and assembly that will allow direct biochemical assay of SurA and related proteins.
  7. IMMUNOPROTECTIVE POTENTIAL OF PORINS AND ANTI-PORIN MABS
    Shiva Singh; Fiscal Year: 1991
    ..This proposal deals with the study of the immunoprotective potential of Salmonella typhimurium porins and anti-porin monoclonal antibodies in experimental murine salmonellosis...
  8. What controls the thickness of biological membranes
    Jonathan Sachs; Fiscal Year: 2006
    ..A set of molecular dynamics simulations will be performed on the OmpF porin in an explicit bilayer setting...
  9. EFFECT OF PORA ON MENINGOCOCCI-MACROPHAGE INTERACTION
    Lillian Waldbeser; Fiscal Year: 1999
    ..Bacterial outer membrane proteins play a part in this interaction. The porin PorA is known to be capable of insertion into mammalian membranes, and is known to alter phagocyte functions...
  10. REGULATION OF PULMONARY CIRCULATION IN FETUS AND NEWBORN
    Girija G Konduri; Fiscal Year: 2013
    ..and postnatal oxygen tension stimulate the association of eNOS with the mitochondrial outer membrane protein, porin in normal fetal lamb pulmonary artery endothelial cells (PAEC)...
  11. ERIC KIRK LAU; Fiscal Year: 2014
    ..ATF2 induces mitochondrial membrane leakage by perturbing hexokinase1 and voltage-dependent anion channel 1-(HK1:VDAC1) containing complexes, and activating pro-apoptotic Bcl2 protein, Bax...
  12. Redesign of the Carbapenem Scaffold
    Bret M Benton; Fiscal Year: 2012
    ..B metallo-carbapenemases, such as the NDM-1 enzyme, can be combined with other resistance determinants, such as porin deletions and upregulated efflux systems to generate a microorganism that is resistant to virtually all known ..
  13. Effect of Neisserial Porin in Immune Cell Apoptosis
    Lee Wetzler; Fiscal Year: 2006
    ..We found that the anti-apoptotic effect is due to a direct interaction of the porin with mitochondria (a central control point for apoptotic cell death)...
  14. Nickolay Brustovetsky; Fiscal Year: 2015
    ..The overall objectives of the proposed study are to clarify the role of mitochondrial porin, also known as voltage-dependent anion channel (VDAC), in mutant huntingtin (mHtt)-induced mitochondrial ..
  15. Role of mitochondria in cardiac protection
    Peipei Ping; Fiscal Year: 2010
    ..Furthermore, our preliminary data demonstrate for the first time that multiple isoforms of VDAC (VDAC1-3) and ANT (ANT1-2) are expressed in the adult mouse heart as detected by both immunoblotting (using antibodies we ..
  16. IMMUNOLOGY OF INFECTION WITH NEISSERIA GONORRHOEAE
    Peter A Rice; Fiscal Year: 2013
    ..The porin molecule also binds factor H...
  17. Maha Abdellatif; Fiscal Year: 2016
    ..g. Vdac1, pinin, TFIIB, Cdk9, MAPK1, etc)...
  18. Rescuing CTL from Activation Induced Death
    Bijay Mukherji; Fiscal Year: 2010
    ..on mitochondria and to interact with several mitochondria-based Bcl-2 family proteins and with the mitichondrial porin voltage dependent anion channel (VDAC)...
  19. IMMUNOLOGY OF INFECTION WITH NEISSERIA GONORRHOEAE
    Peter Rice; Fiscal Year: 2003
    ..complement pathway regulatory protein factor H to sialylated lipooligosaccharide (LOS) and to loop 5 of porin protein (Por) IA...
  20. GONOCOCCAL PIA--ROLE IN INVASION OF FALLOPIAN TUBE CELL
    GARY GORBY; Fiscal Year: 1999
    ..GC Por or PI, the focus of this proposal, is an outer membrane porin protein that comes in two forms, PIA or PIB. It does not vary within a single GC strain...
  21. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2003
    ..ever three-dimensional solution structure of the histidine kinase domain of EnvZ, an osmosensor that regulates porin gene expression (ompF and ompC)...
  22. Transcytosis of Neisseria gonorrhoeae through T84 epithelial cells
    LUZ ANGELA LE VAN; Fiscal Year: 2009
    ..Many molecular determinants have been implicated in gonococcal pathogenesis such as porin, pili, lipooligosaccharide (LOS) and colony associated opacity proteins (Opa)...
  23. Neisseria gonorrhoeae enhances HIV - 1 infection
    Tie Chen; Fiscal Year: 2006
    ..When GC enters the hosts, it releases components, such as lipopolysaccharide (LPS), lipoprotein H.8, porin and peptidoglycan (PGN), which can activate Toll-like receptors (TLRs)...
  24. Mary Jackson; Fiscal Year: 2015
    ..In support of these assumptions, we recently demonstrated that reduced porin expression was one of the mechanisms through which M. smegmatis and M...
  25. Assay Development for Inhibitors of the Essential sRNA-Sigma E Virulence Factors
    Sarah E Ades; Fiscal Year: 2010
    ..small molecule inhibitors of two key steps in the sE pathway: sE-dependent transcription, and regulation of porin mRNA levels by the protein Hfq in conjunction with sE-dependent sRNAs...
  26. Regulation of Vibrio cholerae virulence by ToxR and TcpP
    Eric S Krukonis; Fiscal Year: 2010
    ..Expression of the porin, OmpU, leads to bile resistance in V. cholerae...
  27. Characterization of OmpR Gene Regulation
    Linda J Kenney; Fiscal Year: 2010
    ..The two-component system in E. coli that regulates the porin genes responds to changes in osmolarity of the growth medium...
  28. LEE MARK WETZLER; Fiscal Year: 2016
    ..in this mouse model, and 3) Evaluate the efficacy of gonococcal vaccine candidates, including gonococcal porin, gonococcal outer membrane vesicles, and other outer membrane components defined by Novartis Vaccines (see letter)...
  29. High Throughput Protein Production by Novel E. coli Expression-Secretion System
    WILLIAM MACCONNELL; Fiscal Year: 2006
    ..Published work has shown that fusion of coding regions such as: scFv antibody chain, E. coli outer membrane porin (OmpF), chloramphenicol acetyltransferase (CAT), or L-asparagenase genes to the c-terminal 200 amino acid portions ..
  30. Structure and interactions of the Voltage-Dependent Anion Channel(VDAC)
    Gerhard Wagner; Fiscal Year: 2013
    ..4. Characterize complexes of VDAC with the porin PorB from pathogenic bacteria...
  31. PATRICIA LOUISE CLARK; Fiscal Year: 2014
    ..2) We will determine whether the AT passenger exits the cell through its own C-terminal porin domain, or through another OM porin such as BamA...
  32. PATHOGENESIS OF LIVER CELL NECROSIS
    John Farber; Fiscal Year: 2005
    ..and apoptosis direct physical connections through the cytoskeleton between the outer mitochondrial membrane (porin in particular) and the plasma membrane mediate the energy-independent coupling of the MPT to the loss of viability ..
  33. Development of Paratransgenic ticks for Disease Control
    Ulrike Munderloh; Fiscal Year: 2001
    ..Our long term aim is the stable transformation of Rickettsia Peacockii with the cecropinA an insect porin gene, infection of ticks with the transformed rickettsia, and interference with pathogen transmission...
  34. ROLE OF HEXOKINASE IN LUNG ADAPTATION TO OXIDANT STRESS
    Carl White; Fiscal Year: 2002
    ..cells, (2) Define the mitochondrial association or lack thereof of these hexokinase isoforms and the expression of porin proteins to which they bind, and (3) Clarify the potential importance of HK-II expression in adaptation and ..
  35. Michael Niederweis; Fiscal Year: 2016
    ..With funding through this program, our group has engineered the porin MspA specifically for nanopore sequencing...
  36. PATHOGENIC ROLES OF COXIELLA BURNETII SURFACE PROTEINS
    James Samuel; Fiscal Year: 1999
    ..and is therefore likely to function in membrane integrity, resistance to complement-mediated killing and as a porin. P2, a putative lipoprotein, may function as other bacterial lipoproteins to induce inflammatory cytokines...
  37. The Role of Apoptosis in CryABR120G Heart Failure
    James Pattison; Fiscal Year: 2009
    ..Specific Aim 1 will test the hypothesis that VDAC1 is necessary for CryABRI 20G induced mitochondrial dysfunction...
  38. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 2001
    DESCRIPTION: Synthesis of the two major porin protein of Escherichia coli, OmpF and OmpC, is controlled by complex, overlapping regulatory mechanisms that each respond to key environmental cues...
  39. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2000
    ..The diffusion of hydrophilic solutes are mediated by porin and other specific channels, and the properties of these channels will be characterized...
  40. HEXOKINASE AND ENERGY METABOLISM IN THE BRAIN
    John Wilson; Fiscal Year: 2002
    ..coupling of hexokinase to adenine nucleotide transport in planar lipid bilayers containing purified mitochondrial porin; 5...
  41. IMMUNE MECHANISMS IN EXPERIMENTAL SYPHILIS
    Michael Lovett; Fiscal Year: 2004
    ..OMV porin activity resides in a 31 kDa protein, Tromb1...
  42. DIAGNOSTIC ASSAYS FOR TYPHOID FEVER
    Felipe Cabello; Fiscal Year: 1993
    The molecular cloning and the expression of the Salmonella typhi 36 kDal porin in Escherichia coli, the sequence analysis of its gene, the preparation of monoclonal antibodies against this antigen, and immunoblots and ..
  43. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2009
    ..We will also analyze the reciprocal regulatory mechanism for the ompF and ompC outer membrane porin genes at the level of transcription by OmpR-P and at the level of translation by two antisense RNAs, micF and micC ..
  44. OUTER MEMBRANE PROTEINS OF PATHOGENIC LEPTOSPIRA SPECIES
    David Haake; Fiscal Year: 2001
    ..When expressed as recombinant membrane proteins, the combination of the leptospiral porin OmpL1, and a surface-exposed lipoprotein, LipL4l are synergistically immunoprotective in the hamster model of ..
  45. MEMBRANE INTERACTIONS BETWEEN BDELLOVIBRIO AND ITS PREY
    DANA DIEDRICH; Fiscal Year: 1992
    ..Intraperiplasmic bdellovibrios relocalize to their own outer membranes intact major outer membrane porin proteins purloined from their prey...
  46. SITE DIRECTED SPIN LABELING OF FEPA
    Jimmy Feix; Fiscal Year: 2002
    ..of an entire transmembrane beta- strand, the investigators hypothesize that FepA functions as a ligand-gated porin with surface loops that bind the ligand and regulate access to an underlying transmembrane channel and that ..
  47. STRUCTURE AND FUNCTION OF PEPTIDE INCLUSION COMPLEXES
    BALTHASAR GISIN; Fiscal Year: 1980
    ..b>Porin, an outer membrane protein of E. coli will be reconstituted in artificial membranes...
  48. PATHOGENIC ROLE OF BACTERIAL SURFACE PROTEINS
    Lee Wetzler; Fiscal Year: 1990
    ..We will look at the function of this porin, its importance in pathogenesis...
  49. Design, preparation, and assay of synthetic CI channel
    GEORGE GOKEL; Fiscal Year: 2006
    Channel or "porin" molecules reside in cellular membranes and regulate the flow of cations, anions, water, and other molecular species through the bilayer...
  50. STRUCTURE/FUNCTION OF MEMBRANE PROTEIN CHANNELS
    ALOK MITRA; Fiscal Year: 2002
    ..This will allow us to understand the binding of LF and test the proposed porin-like model for the membrane-embedded domain of PA63...
  51. MEMBRANE PROTEINS--HIGH RESOLUTION ELECTRON MICROSCOPY
    Robert Glaeser; Fiscal Year: 1993
    ..5-year period of this grant will focus on bacteriorhodopsin and the various intermediates in its photocycle, PhoE porin, LamB porin, the adenine nucleotide exchanged protein from mitochondria, and a plasma membrane proton ATPase...
  52. Mechanism of OmpR-dependent Transcriptional Activation
    Don Walthers; Fiscal Year: 2004
    ..In E. coli, the EnvZ-OmpR two-component system regulates transcription of the outer membrane porin genes ompF and ompC, and the csg locus (required for biofilm formation), in response to changes in medium ..
  53. The Role of Polyhydroxybutyrate in OmpA Sorting
    ROSETTA REUSCH; Fiscal Year: 2005
    ..Site-directed mutagenesis of this residue produced cPIIB-minus OmpA. OmpA is also widely regarded as a porin, but the question of whether it is capable of forming open pores remains controversial...
  54. DISULFIDE BRIDGE EPITOPES IN CHLAMYDIAL MOMP
    You Xun Zhang; Fiscal Year: 2004
    ..confers structural rigidity to the infectious form of the organism and exhibits characteristics consistent with porin activity...
  55. Structural studies of membrane proteins by NMR
    MAXIM POMETUN; Fiscal Year: 2007
    ..Using the largely helical KcsA potassium channel and the largely beta-sheet OmpA porin as model systems, we will refine the methods of reverse micelle encapsulation of membrane proteins...
  56. 2008 Bacterial Cell Surfaces Gordon Research Conference
    ANNE DELCOUR; Fiscal Year: 2008
    ..Success in these research endeavors is enhanced by the exchange of information and ideas promoted by the Bacterial Cell Surfaces Gordon Conference. [unreadable] [unreadable] [unreadable]..
  57. MOLECULAR PROPERTIES AND MODULATION OF BACTERIAL PORINS
    ANNE DELCOUR; Fiscal Year: 2003
    ..Changes in porin expression patterns have also been observed in virulent strains of E. coli causing urinary tract infections...
  58. Neisseria meningitidis antigens expressed in infection
    Dan Granoff; Fiscal Year: 2005
    ..meningitidis disease, including group B strains for which there is currently no vaccine available. Also, characterization of gene expression in an in vivo model will lead to a better understanding of meningococcal pathogenesis. ..
  59. 2008 Spirochetes Gordon Research Conference
    David Haake; Fiscal Year: 2008
    ..Funding from the National Institutes of Health is requested to partially offset the travel and registration expenses of the participating graduate students, fellows, and junior faculty members. ..
  60. Molecular basis meningococcal group A capsular immunity
    Dan Granoff; Fiscal Year: 2007
    ..abstract_text> ..
  61. STRUCTURE AND ACTION OF STEROIDOGENIC REGULATORY PROTEIN
    HIMANGSHU BOSE; Fiscal Year: 2002
    ..Fulfilling these aims will provide the structural information needed to develop a detailed understanding of how StAR and MLN64 regulate cholesterol movement from the outer to inner mitochondrial membrane. ..