VASP

Summary

Gene Symbol: VASP
Description: vasodilator-stimulated phosphoprotein
Alias: vasodilator-stimulated phosphoprotein
Species: human

Top Publications

  1. doi Characterization of a novel interaction between vasodilator-stimulated phosphoprotein and Abelson interactor 1 in human platelets: a concerted computational and experimental approach
    Marcus Dittrich
    Department of Bioinformatics, Biocenter, University of Wurzburg, Wurzburg, Germany
    Arterioscler Thromb Vasc Biol 30:843-50. 2010
  2. pmc Loss of profilin-1 expression enhances breast cancer cell motility by Ena/VASP proteins
    Yong Ho Bae
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    J Cell Physiol 219:354-64. 2009
  3. pmc PtdIns(4,5)P-restricted plasma membrane localization of FAN is involved in TNF-induced actin reorganization
    Dirk Haubert
    Institute for Medical Microbiology, Immunology and Hygiene, Medical Faculty, University of Cologne, Cologne, Germany
    EMBO J 26:3308-21. 2007
  4. pmc The enzymatic activity of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase is enhanced by NPM-ALK: new insights in ALK-mediated pathogenesis and the treatment of ALCL
    Francesco E Boccalatte
    Center for Experimental Research and Medical Studies CERMS, University of Torino, Via Santena 5, 10126 Torino, Italy
    Blood 113:2776-90. 2009
  5. pmc The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts
    M Reinhard
    Medizinische Universitatsklinik, Universitat Wurzburg, FRG
    EMBO J 11:2063-70. 1992
  6. pmc Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein)
    M Reinhard
    Medizinische Universitatsklinik, Klinische Biochemie und Pathobiochemie, Wurzburg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 92:7956-60. 1995
  7. pmc Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP
    C Haffner
    Medizinische Universitatsklinik, Klinische Forschergruppe, Wurzburg, Germany
    EMBO J 14:19-27. 1995
  8. ncbi cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets
    E Butt
    Medizinische Universitatsklinik, Klinische Biochemie und Pathobiochemie, Federal Republic of Germany
    J Biol Chem 269:14509-17. 1994
  9. pmc The focal-adhesion vasodilator-stimulated phosphoprotein (VASP) binds to the proline-rich domain in vinculin
    N P Brindle
    Department of Surgery, University of Leicester, U K
    Biochem J 318:753-7. 1996
  10. pmc Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes
    V Laurent
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Cell Biol 144:1245-58. 1999

Research Grants

  1. Russell E McConnell; Fiscal Year: 2014
  2. Role of Protein Kinase D in Actin Remodeling and Cell Motiliy
    Peter Storz; Fiscal Year: 2013
  3. The role of RIAM in T cell immunity and tolerance
    Vassiliki A Boussiotis; Fiscal Year: 2013
  4. Mary C Beckerle; Fiscal Year: 2015
  5. HORMONAL REGULATION OF MESSENGER RNA STABILITY
    Daniel R Schoenberg; Fiscal Year: 2013
  6. Protein interactions in cardiovascular signaling
    Thomas Michel; Fiscal Year: 2009
  7. Frederick Southwick; Fiscal Year: 2014
  8. Cytoskeletal Regulation During Growth Cone Migration and Axon Guidance
    Frank B Gertler; Fiscal Year: 2012
  9. Fat1 and Conventional Kinesin in Lateral Polarity
    DUNCAN BRUCE JOHNSTONE; Fiscal Year: 2012
  10. Cellular and Developmental Function of Mena
    Frank B Gertler; Fiscal Year: 2012

Detail Information

Publications212 found, 100 shown here

  1. doi Characterization of a novel interaction between vasodilator-stimulated phosphoprotein and Abelson interactor 1 in human platelets: a concerted computational and experimental approach
    Marcus Dittrich
    Department of Bioinformatics, Biocenter, University of Wurzburg, Wurzburg, Germany
    Arterioscler Thromb Vasc Biol 30:843-50. 2010
    The goal of this study was systematic profiling of vasodilator-stimulated phosphoprotein (VASP)-Ena/VASP homology 1 (EVH1) interactors in human platelets using a combined in silico and in vitro approach.
  2. pmc Loss of profilin-1 expression enhances breast cancer cell motility by Ena/VASP proteins
    Yong Ho Bae
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    J Cell Physiol 219:354-64. 2009
    ..Interestingly, MDA-MB-231 cells showed dramatic enrichment of VASP at their leading edge when Pfn1 expression was downregulated and this observation was also reproducible in other ..
  3. pmc PtdIns(4,5)P-restricted plasma membrane localization of FAN is involved in TNF-induced actin reorganization
    Dirk Haubert
    Institute for Medical Microbiology, Immunology and Hygiene, Medical Faculty, University of Cologne, Cologne, Germany
    EMBO J 26:3308-21. 2007
    ..The results of this study suggest that PH-mediated plasma membrane targeting of FAN is critically involved in TNF-induced Cdc42 activation and cytoskeleton reorganization...
  4. pmc The enzymatic activity of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase is enhanced by NPM-ALK: new insights in ALK-mediated pathogenesis and the treatment of ALCL
    Francesco E Boccalatte
    Center for Experimental Research and Medical Studies CERMS, University of Torino, Via Santena 5, 10126 Torino, Italy
    Blood 113:2776-90. 2009
    ..Prediction of tumor responses to methotrexate may justify specific molecular-based chemotherapy...
  5. pmc The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts
    M Reinhard
    Medizinische Universitatsklinik, Universitat Wurzburg, FRG
    EMBO J 11:2063-70. 1992
    ..activation by pathways sharing at least one component, the 46/50 kDa vasodilator-stimulated phosphoprotein (VASP)...
  6. pmc Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein)
    M Reinhard
    Medizinische Universitatsklinik, Klinische Biochemie und Pathobiochemie, Wurzburg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 92:7956-60. 1995
    b>VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic ..
  7. pmc Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP
    C Haffner
    Medizinische Universitatsklinik, Klinische Forschergruppe, Wurzburg, Germany
    EMBO J 14:19-27. 1995
    The vasodilator-stimulated phosphoprotein (VASP), a substrate for cAMP- and cGMP-dependent protein kinases in vitro and in intact cells, is associated with actin filaments, focal adhesions and dynamic membrane regions...
  8. ncbi cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets
    E Butt
    Medizinische Universitatsklinik, Klinische Biochemie und Pathobiochemie, Federal Republic of Germany
    J Biol Chem 269:14509-17. 1994
    The vasodilator-stimulated phosphoprotein (VASP) is a major substrate for cAMP-dependent- (cAK) and cGMP-dependent protein kinase (cGK) in human platelets and other cardiovascular cells...
  9. pmc The focal-adhesion vasodilator-stimulated phosphoprotein (VASP) binds to the proline-rich domain in vinculin
    N P Brindle
    Department of Surgery, University of Leicester, U K
    Biochem J 318:753-7. 1996
    In mammalian cells vasodilator-stimulated phosphoprotein (VASP) is localized to focal adhesions and areas of dynamic membrane activity where it is thought to have a role in actinfilament assembly...
  10. pmc Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes
    V Laurent
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Cell Biol 144:1245-58. 1999
    ..and brain extracts to elucidate the function of the focal adhesion proteins of the Ena (Drosophila Enabled)/VASP (vasodilator-stimulated phosphoprotein) family in actin-based motility...
  11. ncbi The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation
    C Bachmann
    Institut für Klinische Biochemie und Pathobiochemie, Medizinische Universitatsklinik, Versbacher Strasse 5, D 97078 Wurzburg, Germany
    J Biol Chem 274:23549-57. 1999
    Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena/VASP family of proteins that are implicated in regulation of the actin cytoskeleton...
  12. pmc Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity
    L J Ball
    Forschungsinstitut fur Molekulare Pharmakologie, Alfred Kowalke Strasse 4, D 10315, Berlin, Germany
    EMBO J 19:4903-14. 2000
    The Ena-VASP family of proteins act as molecular adaptors linking the cytoskeletal system to signal transduction pathways...
  13. pmc VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations
    E L Bearer
    Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island 02912, USA
    Cell Motil Cytoskeleton 47:351-64. 2000
    ..Shape change is inhibited by VASP, another abundant actin-binding protein...
  14. pmc A WASp-VASP complex regulates actin polymerization at the plasma membrane
    F Castellano
    Laboratoire de la Dynamique de la Membrane et du Cytosquelette, Centre National de la Recherche Scientifique UMR 144, Institut Curie, 26 Rue d Ulm, 75241 Paris Cedex 5, France
    EMBO J 20:5603-14. 2001
    Proteins of the Wiskott-Aldrich syndrome and Ena/VASP families both play essential functions in the regulation of actin dynamics at the cell leading edge...
  15. pmc How VASP enhances actin-based motility
    Stanislav Samarin
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Cell Biol 163:131-42. 2003
    The function of vasodilator-stimulated phosphoprotein (VASP) in motility is analyzed using a biomimetic motility assay in which ActA-coated microspheres propel themselves in a medium containing actin, the Arp2/3 complex, and three ..
  16. ncbi Myosin X transports Mena/VASP to the tip of filopodia
    Hiroshi Tokuo
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Biochem Biophys Res Commun 319:214-20. 2004
    ..Here we found that M10 binds to Mena/VASP, which facilitates actin polymerization by competing with actin capping proteins...
  17. pmc Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins
    Melanie Barzik
    Department of Biology, University of Virginia, Charlottesville, Virginia 22903, USA
    J Biol Chem 280:28653-62. 2005
    Ena/VASP proteins influence the organization of actin filament networks within lamellipodia and filopodia of migrating cells and in actin comet tails. The molecular mechanisms by which Ena/VASP proteins control actin dynamics are unknown...
  18. ncbi Migfilin interacts with vasodilator-stimulated phosphoprotein (VASP) and regulates VASP localization to cell-matrix adhesions and migration
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:12397-407. 2006
    ..Molecularly, we have identified vasodilator-stimulated phosphoprotein (VASP) as a new migfilin-binding protein...
  19. pmc Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP
    Francois Ferron
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    EMBO J 26:4597-606. 2007
    ..Filament elongation factors such as Ena/VASP and formin catalyze the transition of profilin-actin from the cellular pool onto the barbed end of growing ..
  20. ncbi Positive regulation of migration and invasion by vasodilator-stimulated phosphoprotein via Rac1 pathway in human breast cancer cells
    Guoge Han
    Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Allergy and Immune Related Diseases Centre for Medical Research, School of Medicine, Wuhan University, Hubei, P R China
    Oncol Rep 20:929-39. 2008
    ..study aimed to investigate the role of the cytoskeleton-associated protein vasodilator-stimulated phosphoprotein (VASP) on the migration and invasion of human breast cancer cells and its relationship to Rac1 which is a member of the ..
  21. pmc Clustering of VASP actively drives processive, WH2 domain-mediated actin filament elongation
    Dennis Breitsprecher
    Institute for Biophysical Chemistry, Hannover Medical School, Hannover, Germany
    EMBO J 27:2943-54. 2008
    Vasodilator-stimulated phosphoprotein (VASP) is a key regulator of dynamic actin structures like filopodia and lamellipodia, but its precise function in their formation is controversial...
  22. pmc VASP is a CXCR2-interacting protein that regulates CXCR2-mediated polarization and chemotaxis
    Nicole F Neel
    Department of Veterans Affairs, Nashville, TN 37212, USA
    J Cell Sci 122:1882-94. 2009
    ..Using this approach, vasodilator-stimulated phosphoprotein (VASP) was identified as a CXCR2-interacting protein...
  23. pmc Inflammation-associated repression of vasodilator-stimulated phosphoprotein (VASP) reduces alveolar-capillary barrier function during acute lung injury
    Janek Henes
    Department of Anesthesiology and Intensive Care Medicine, Tuebingen University Hospital, Tuebingen, Germany
    FASEB J 23:4244-55. 2009
    ..A protein central to physiological barrier properties is vasodilator-stimulated phosphoprotein (VASP)...
  24. pmc VASP is a processive actin polymerase that requires monomeric actin for barbed end association
    Scott D Hansen
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco School of Medicine, San Francisco, CA 94158, USA
    J Cell Biol 191:571-84. 2010
    Ena/VASP proteins regulate the actin cytoskeleton during cell migration and morphogenesis and promote assembly of both filopodial and lamellipodial actin networks...
  25. doi Abl-1-bridged tyrosine phosphorylation of VASP by Abelson kinase impairs association of VASP to focal adhesions and regulates leukaemic cell adhesion
    Masahiro Maruoka
    Laboratory of Single Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Biochem J 441:889-99. 2012
    Mena [mammalian Ena (Enabled)]/VASP (vasodilator-stimulated phosphoprotein) proteins are the homologues of Drosophila Ena. In Drosophila, Ena is a substrate of the tyrosine kinase DAbl (Drosophila Abl)...
  26. doi HIF-1α acts downstream of TNF-α to inhibit vasodilator-stimulated phosphoprotein expression and modulates the adhesion and proliferation of breast cancer cells
    Ke Su
    Department of Pathology and Pathophysiology, Institute of Allergy and Immune related Diseases, Centre for Medical Research, Wuhan University School of Medicine, Wuhan, People s Republic of China
    DNA Cell Biol 31:1078-87. 2012
    ..through hypoxia inducible factor-1alpha (HIF-1α) mediated suppression of vasodilator-stimulated phosphoprotein (VASP)...
  27. ncbi Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins
    B Drees
    Huntsman Cancer Institute, Department of Biology, University of Utah, Salt Lake City, Utah 84103, USA
    J Biol Chem 275:22503-11. 2000
    ..sequences were the sole zyxin sequences capable of binding to Ena/vasodilator-stimulated phosphoprotein (VASP) family members in vitro...
  28. ncbi Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics
    Matthias Krause
    Department of Biology and Center for Cancer Research, MIT, Cambridge, MA 02139, USA
    Dev Cell 7:571-83. 2004
    Lamellipodial protrusion is regulated by Ena/VASP proteins. We identified Lamellipodin (Lpd) as an Ena/VASP binding protein. Both proteins colocalize at the tips of lamellipodia and filopodia...
  29. pmc The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat
    Karin Kühnel
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Proc Natl Acad Sci U S A 101:17027-32. 2004
    The vasodilator-stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3-A resolution crystal structure of the 45-residue-long tetramerization domain (TD) from human VASP...
  30. ncbi The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin
    Birgit Walders-Harbeck
    Cell Biology, Zoological Institute, Technical University of Braunschweig, Biocenter, Spielmannstrasse 7, D 38092 Braunschweig, Germany
    FEBS Lett 529:275-80. 2002
    The vasodilator-stimulated phosphoprotein (VASP) functions as a cellular regulator of actin dynamics. VASP may initialise actin polymerisation, suggesting a direct interaction with monomeric actin...
  31. ncbi Analysis of GTP-binding proteins, phosphoproteins, and cytosolic calcium in functional heterogeneous human blood platelet subpopulations
    C Opper
    Institute of Physiological Chemistry, University of Marburg, Germany
    Biochem Pharmacol 54:1027-35. 1997
    ..protein phosphorylation, cytoplasmic calcium ([Ca2+]i), the ratio of 46 and 50 kDa vasodilator-stimulated protein (VASP), and GTP-binding proteins (G-proteins). Platelets were fractionated by density...
  32. doi Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells
    Olivia Knauer
    Junior Research Group, Paul Ehrlich Institute, Paul Ehrlich Str 51 59, D 63225 Langen, Germany
    Cell Microbiol 10:2285-96. 2008
    ..We analysed functions of one identified H. pylori-regulated candidate, the vasodilator-stimulated phosphoprotein (VASP). H...
  33. ncbi Von Willebrand factor activates endothelial nitric oxide synthase in blood platelets by a glycoprotein Ib-dependent mechanism
    R Riba
    Medical Biosciences, University of Bradford, Bradford, West Yorkshire, UK
    J Thromb Haemost 4:2636-44. 2006
    ..In particular, the ability of von Willebrand factor (VWF) to stimulate cyclic guanosine monophosphate (cGMP) formation in platelets has produced conflicting data...
  34. pmc Alterations in vasodilator-stimulated phosphoprotein (VASP) phosphorylation: associations with asthmatic phenotype, airway inflammation and beta2-agonist use
    Annette T Hastie
    Department of Internal Medicine, Center for Human Genomics, Wake Forest University School of Medicine, Winston Salem, NC, USA
    Respir Res 7:25. 2006
    Vasodilator-stimulated phosphoprotein (VASP) mediates focal adhesion, actin filament binding and polymerization in a variety of cells, thereby inhibiting cell movement...
  35. ncbi Does nitric oxide mediate the vasodilator activity of nitroglycerin?
    Andrei L Kleschyov
    Division of Cardiology, University Hospital Hamburg Eppendorf, Martinistr 52, Hamburg 20246, Germany
    Circ Res 93:e104-12. 2003
    ..and (2) analysis of cGK-I-dependent phosphorylation of the vasodilator-stimulated phosphoprotein at Ser239 (P-VASP)...
  36. doi Globular adiponectin increases cGMP formation in blood platelets independently of nitric oxide
    R Riba
    Centre for Atherothrombosis Research, Medical Biosciences, University of Bradford, Bradford, UK
    J Thromb Haemost 6:2121-31. 2008
    ..This confusion concerning the roles of platelet NO may have arisen because of an uncharacterized mechanism for activation of sGC...
  37. ncbi Universal varicella vaccination: efficacy trends and effect on herpes zoster
    Gary S Goldman
    Medical Veritas International MVI, Pearblossom, California 93553, USA
    Int J Toxicol 24:205-13. 2005
    In 1995, the Varicella Active Surveillance Project (VASP) was established in Antelope Valley (California), a geographically distinct high-desert community of 300,000 residents, as one of three sites in the nation in a cooperative ..
  38. doi The effect of antiplatelet drugs clopidogrel and aspirin is less immediately after stent implantation
    Jolanta M Siller-Matula
    Department of Clinical Pharmacology, Medical University of Vienna, Austria
    Thromb Res 123:874-80. 2009
    ..The aim was to investigate if the inhibition of platelet function by clopidogrel and aspirin is equal at two different time points: immediately after percutaneous coronary intervention (PCI) and one day thereafter...
  39. ncbi Regulation of VASP serine 157 phosphorylation in human neutrophils after stimulation by a chemoattractant
    Rachael E Eckert
    Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    J Leukoc Biol 82:1311-21. 2007
    Vasodilator-stimulated phosphoprotein (VASP) is a cAMP-dependent protein kinase A (PKA) substrate, which links cellular signaling to cytoskeletal organization and cellular movement...
  40. pmc Regulation of VASP phosphorylation in cardiac myocytes: differential regulation by cyclic nucleotides and modulation of protein expression in diabetic and hypertrophic heart
    Juliano L Sartoretto
    Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Am J Physiol Heart Circ Physiol 297:H1697-710. 2009
    Vasodilator-stimulated phosphoprotein (VASP) is a major substrate for cyclic nucleotide-dependent kinases that has been implicated in cardiac pathology, yet many aspects of VASP's molecular regulation in cardiomyocytes are incompletely ..
  41. doi Peroxynitrite causes phosphorylation of vasodilator-stimulated phosphoprotein through a PKC dependent mechanism
    Ahmed Aburima
    Hull York Medical School, University of Hull, Castle Hill Hospital, Castle Road, Cottingham HU67RX, UK
    Platelets 21:421-8. 2010
    ..In the present study we investigated the influence of peroxynitrite on vasodilator-stimulated phosphoprotein (VASP), a protein that plays a key role in inhibition of platelet adhesion and spreading...
  42. pmc Roles for soluble guanylate cyclase and a thiol oxidation-elicited subunit dimerization of protein kinase G in pulmonary artery relaxation to hydrogen peroxide
    Boon Hwa Neo
    Department of Physiology, New York Medical College, Valhalla, NY 10595, USA
    Am J Physiol Heart Circ Physiol 299:H1235-41. 2010
    ..These responses were associated with increased phosphorylation of vasodilator-stimulated phosphoprotein (VASP) at the serine-239 site known to be mediated by PKG...
  43. ncbi Vesl/Homer proteins regulate ryanodine receptor type 2 function and intracellular calcium signaling
    Jens H Westhoff
    Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA
    Cell Calcium 34:261-9. 2003
    ..such as metabotropic glutamate receptors, Shank, and different types of ion channels are physically linked by Vesl (VASP/Ena-related gene up-regulated during seizure and LTP)/Homer proteins [Curr. Opin. Neurobiol...
  44. ncbi Ena/VASP proteins mediate repulsion from ephrin ligands
    Iwan R Evans
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    J Cell Sci 120:289-98. 2007
    Ena/VASP proteins negatively regulate cell motility and contribute to repulsion from several guidance cues; however, there is currently no evidence for a role downstream of Eph receptors...
  45. doi Platelet inhibition assessed with VerifyNow, flow cytometry and PlateletMapping in patients undergoing heart surgery
    Ulrica Alström
    Department of Cardiac and Thoracic Anesthesia and Surgery, Uppsala University Hospital, SE 75185 Uppsala, Sweden
    Thromb Res 124:572-7. 2009
    ....
  46. pmc Actin polymerization in differentiated vascular smooth muscle cells requires vasodilator-stimulated phosphoprotein
    Hak Rim Kim
    Dept of Health Sciences, Boston Univ, 635 Commonwealth Ave, Boston MA 02215, USA
    Am J Physiol Cell Physiol 298:C559-71. 2010
    ..Here, we evaluated the possible functions of the Ena/vasodilator-stimulated phosphoprotein (VASP) family of actin filament elongation factors in dVSMC...
  47. ncbi The activity of constitutive nitric oxide synthase is increased by the pathway cAMP/cAMP-activated protein kinase in human platelets. New insights into the antiaggregating effects of cAMP-elevating agents
    Isabella Russo
    Diabetes Unit, Department of Clinical and Biological Sciences of the Turin University, Ospedale San Luigi Gonzaga Hospital, Regione Gonzole, 10, Orbassano 10043, Turin, Italy
    Thromb Res 114:265-73. 2004
    ..The phosphorylation of the PKA substrate vasodilator-stimulated phosphoprotein (VASP) at Ser(157) and Ser(239) and of ecNOS at Ser(1177) was evaluated by Western blot...
  48. ncbi Cyclic AMP- and cyclic GMP-dependent protein kinases differ in their regulation of cyclic AMP response element-dependent gene transcription
    S P Collins
    Department of Biological Chemistry and the Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 274:8391-404. 1999
    ..kinase (cGKIbeta) and the type II cAMP-dependent protein kinase (cAKII) phosphorylated the cytoplasmic substrate VASP (vasodilator- and A kinase-stimulated phosphoprotein) to a similar extent, cyclic nucleotide regulation of CRE-..
  49. doi Pharmacodynamics and pharmacokinetics of single doses of prasugrel 30 mg and clopidogrel 300 mg in healthy Chinese and white volunteers: an open-label trial
    David S Small
    Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana 46285, USA
    Clin Ther 32:365-79. 2010
    ..Although the approved loading dose is 60 mg, earlier studies of prasugrel suggested that active-metabolite exposure and pharmacodynamic response may be higher in Asian subjects than in white subjects...
  50. pmc Caenorhabditis elegans WASP and Ena/VASP proteins play compensatory roles in morphogenesis and neuronal cell migration
    Jim Withee
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Genetics 167:1165-76. 2004
    We report here that WASP and Ena/VASP family proteins play overlapping roles in C. elegans morphogenesis and neuronal cell migration...
  51. doi Impact of P2Y12-ADP receptor polymorphism on the efficacy of clopidogrel dose-adjustment according to platelet reactivity monitoring in coronary artery disease patients
    Laurent Bonello
    Departement de Cardiologie, Hopital Universitaire Nord, Faculte de Medecine, Marseille, France
    Thromb Res 125:e167-70. 2010
    ..However this strategy failed in nearly 10% of patients with HTPR. We aimed to determine if P2Y12-ADP receptor polymorphisms were associated with failed dose adjustment...
  52. ncbi Identification of cofilin, coronin, Rac and capZ in actin tails using a Listeria affinity approach
    V David
    Unité des Interactions Bactéries Cellules, Institut Pasteur, 75724 Paris Cedex 15, France
    J Cell Sci 111:2877-84. 1998
    ..Cell components known to play a role in the actin-based motility of Listeria are VASP (vasodilatator-stimulated phosphoprotein), the multiprotein Arp2/3 complex and cofilin...
  53. pmc Characterization of human palladin, a microfilament-associated protein
    O M Mykkänen
    Department of Pathology, Helsinki University Hospital University of Helsinki, FIN 00014 Helsinki, Finland
    Mol Biol Cell 12:3060-73. 2001
    ..The N terminus includes an FPPPP motif recognized by the Ena-Vasp homology domain 1 domain in Ena/vasodilatator-stimulated phosphoprotein (VASP)/Wiscott-Aldrich syndrome protein (..
  54. ncbi Resistance to the nitric oxide/cyclic guanosine 5'-monophosphate/protein kinase G pathway in vascular smooth muscle cells from the obese Zucker rat, a classical animal model of insulin resistance: role of oxidative stress
    I Russo
    Diabetes Unit, Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, 10043 Orbassano TO, Italy
    Endocrinology 149:1480-9. 2008
    ..and phosphodiesterases (PDEs); 2) NO and cGMP ability to induce, via PKG, vasodilator-stimulated phosphoprotein (VASP) phosphorylation at serine 239 and PDE5 activity; 3) protein expression of sGC, PKG, total VASP, and PDE5; 4) ..
  55. ncbi Formation of biologically active autacoids is regulated by calcium influx in endothelial cells
    H J Kruse
    Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheifen, University of Munich, FRG
    Arterioscler Thromb 14:1821-8. 1994
    ..cGMP-dependent phosphorylation of two platelet proteins, rap1B and a 50-kD vasodilator-stimulated phosphoprotein (VASP), was analyzed in coincubation experiments of endothelial cells with platelets...
  56. doi Assessment of platelet response to clopidogrel with multiple electrode aggregometry, the VerifyNow P2Y12 analyzer and platelet Vasodilator-Stimulated Phosphoprotein flow cytometry
    Nicolas von Beckerath
    Deutsches Herzzentrum, 1 Medizinische Klinik rechts der Isar, Technische Universitat Munchen, Munich, Germany
    Blood Coagul Fibrinolysis 21:46-52. 2010
    ..The main purpose of the present study was to correlate MEA ADPtest with the VerifyNow P2Y12 analyzer, Platelet VASP flow cytometry and the MEA ADPtest HS in order to test if these assays can substitute for each other...
  57. doi Slow response to clopidogrel predicts low response
    Anne Bellemain-Appaix
    Assistance Publique Hopitaux de Paris, Institut de Cardiologie, Pitie Salpetriere University Hospital, Paris, France
    J Am Coll Cardiol 55:815-22. 2010
    ..The purpose of this study was to determine whether the speed of response to clopidogrel loading predicts the final degree of response...
  58. doi The role of prostanoid receptors in mediating the effects of PGE(2) on human platelet function
    David Iyu
    Cardiovascular Medicine, University of Nottingham, Nottingham, UK
    Platelets 21:329-42. 2010
    ..Their effects on vasodilator-stimulated phosphoprotein (VASP) phosphorylation were also determined...
  59. ncbi Phosphorylation of blood vessel vasodilator-stimulated phosphoprotein at serine 239 as a functional biochemical marker of endothelial nitric oxide/cyclic GMP signaling
    César Ibarra-Alvarado
    Rudolf Buchheim Institute for Pharmacology, Justus Liebig University, Giessen, Germany
    Mol Pharmacol 61:312-9. 2002
    ..analysis and specific antibodies, we show here that phosphorylation of the vasodilator-stimulated phosphoprotein (VASP) at Ser239 (P(Ser239)-VASP) in rabbit aorta was detectable only in segments with an intact endothelium, although at ..
  60. ncbi Increased noise sensitivity and altered inner ear MENA distribution in VASP-/- mice
    Bernhard Schick
    Department of Otolaryngology, University Erlangen Nurnberg, Waldstrasse 1, 91054, Erlangen, Germany
    Cell Tissue Res 318:493-502. 2004
    Vasodilator-stimulated phosphoprotein (VASP) and mammalian-enabled protein (MENA) share similar cellular localisation and functions (signal transduction pathways, regulation of actin cytoskeleton dynamics)...
  61. ncbi High-resolution structural analysis of mammalian profilin 2a complex formation with two physiological ligands: the formin homology 1 domain of mDia1 and the proline-rich domain of VASP
    Petri Kursula
    EMBL Hamburg Outstation, DESY, Notkestrasse 85, D 22607 Hamburg, Germany
    J Mol Biol 375:270-90. 2008
    ..A number of proline-rich ligands for profilin have been characterised, including proteins of the Ena/VASP and formin families...
  62. pmc Cytoskeleton assembly at endothelial cell-cell contacts is regulated by alphaII-spectrin-VASP complexes
    Peter M Benz
    Institute of Clinical Biochemistry and Pathobiochemistry, University of Wurzburg, D 97080 Wurzburg, Germany
    J Cell Biol 180:205-19. 2008
    ..Regulated by phosphorylation, vasodilator-stimulated phosphoprotein (VASP) participates in actin fiber formation...
  63. pmc Vasodilator-stimulated phosphoprotein (VASP) induces actin assembly in dendritic spines to promote their development and potentiate synaptic strength
    Wan Hsin Lin
    Department of Biological Sciences and Vanderbilt Kennedy Center for Research on Human Development, Vanderbilt University, Nashville, Tennessee 37235, USA
    J Biol Chem 285:36010-20. 2010
    ..In this study we show the multifunctional actin-binding protein vasodilator-stimulated phosphoprotein (VASP) regulates the density, size, and morphology of dendritic spines by inducing actin assembly in these structures...
  64. pmc Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice
    W Hauser
    Institut für Klinische Biochemie und Pathobiochemie und, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Proc Natl Acad Sci U S A 96:8120-5. 1999
    Vasodilator-stimulated phosphoprotein (VASP), a substrate of cAMP- and cGMP-dependent protein kinases, is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions...
  65. ncbi X-ray structure determination of human profilin II: A comparative structural analysis of human profilins
    I M Nodelman
    Department of Molecular Biology, Henry H Hoyt Laboratory, Princeton University, Princeton, NJ 08544, USA
    J Mol Biol 294:1271-85. 1999
    ..Various multidomain proteins including members of the Ena/VASP and formin families localize profilin:actin complexes through profilin:poly-L-proline interactions to particular ..
  66. ncbi cAMP-dependent protein kinase phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3 domains
    A Lambrechts
    Flanders Interuniversity Institute for Biotechnology, Department of Medical Protein Chemistry, Faculty of Medicine, Ghent University, Ledeganckstraat 35, 9000 Gent, Belgium
    J Biol Chem 275:36143-51. 2000
    Proteins of the Ena/VASP family are implicated in processes that require dynamic actin remodeling such as axon guidance and platelet activation...
  67. ncbi The orthologous human and murine semaphorin 6A-1 proteins (SEMA6A-1/Sema6A-1) bind to the enabled/vasodilator-stimulated phosphoprotein-like protein (EVL) via a novel carboxyl-terminal zyxin-like domain
    A Klostermann
    MPI of Psychiatry, Independent Research Group Neurodegeneration, Kraepelinstrasse 2, 80804 Munich, Germany
    J Biol Chem 275:39647-53. 2000
    ..Ena/VASP (enabled/vasodilator-stimulated phosphoprotein) proteins play an important role in actin and filament dynamics, ..
  68. ncbi Age-related decrease of protein kinase G activation in vascular smooth muscle cells
    C S Lin
    Knuppe Molecular Urology Laboratory, University of California, San Francisco, CA 94143 1695, USA
    Biochem Biophys Res Commun 287:244-8. 2001
    ..To overcome this difficulty, a monoclonal antibody, VASP-16C2, was recently developed that detects phosphorylated serine residue 239 of vasodilator-stimulated ..
  69. ncbi Distribution, cellular localization, and postnatal development of VASP and Mena expression in mouse tissues
    S Gambaryan
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44, St Petersburg, Russia
    Histochem Cell Biol 116:535-43. 2001
    Vasodilator-stimulated phosphoprotein (VASP) and mammalian Enabled (Mena) are members of the proline-rich Ena/VASP protein family that links the cell membrane proteins, signal transduction pathways, and the actin cytoskeleton...
  70. ncbi Cyclic AMP and cyclic GMP activate protein kinase G in cavernosal smooth muscle cells: old age is a negative factor
    C S Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 1695, USA
    BJU Int 89:576-82. 2002
    ..To investigate protein kinase G-I (PKG-I) expression and activation in cavernosal smooth muscle cells (CSMC) of young and old rats...
  71. ncbi Reduced basal nitric oxide bioavailability and platelet activation in young spontaneously hypertensive rats
    Andreas Schafer
    Medizinische Klinik der Julius Maximilians Universität, Wurzburg, Germany
    Biochem Pharmacol 67:2273-9. 2004
    ..Phosphorylation of the vasodilator-stimulated phosphoprotein (VASP) in platelets was used as a sensitive monitor of in vivo NO bioavailability.
  72. ncbi Vinculin, VASP, and profilin are coordinately regulated during actin remodeling in epithelial cells, which requires de novo protein synthesis and protein kinase signal transduction pathways
    Margaret P Quinlan
    Laboratory of Molecular Cell Biology, Guthrie Research Institute, 1 Guthrie Square, Sayre, Pennsylvania 18840, USA
    J Cell Physiol 200:277-90. 2004
    ..actin network, the expression and localization of the actin binding and regulating proteins (ABPs), vinculin, VASP, and profilin were evaluated...
  73. pmc VASP governs actin dynamics by modulating filament anchoring
    Léa Trichet
    Laboratoire Physicochimie Curie UMR 168 CNRS, Institut Curie Section de Recherche, 11 rue Pierre et Marie Curie, 75231 Paris, Cedex 05, France
    Biophys J 92:1081-9. 2007
    ..an open question, but possible candidates are the Drosophila enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) family of proteins, which localize to dynamic actin structures in the cell...
  74. pmc Adapter protein SH2-Bbeta stimulates actin-based motility of Listeria monocytogenes in a vasodilator-stimulated phosphoprotein (VASP)-dependent fashion
    Maria Diakonova
    Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Infect Immun 75:3581-93. 2007
    ..to Listeria and SH2-Bbeta stimulation of actin-based propulsion require the vasodilator-stimulated phosphoprotein (VASP), which binds ActA at the surfaces of Listeria cells and enhances bacterial actin-based motility...
  75. pmc Ena/VASP function in retinal axons is required for terminal arborization but not pathway navigation
    Asha Dwivedy
    Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
    Development 134:2137-46. 2007
    The Enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) family of proteins is required for filopodia formation in growth cones and plays a crucial role in guidance cue-induced remodeling of the actin cytoskeleton...
  76. pmc Syndecan-2 induces filopodia and dendritic spine formation via the neurofibromin-PKA-Ena/VASP pathway
    Yi Ling Lin
    Faculty of Life Sciences, Institute of Genome Sciences, National Yang Ming University, Taipei 112, Taiwan
    J Cell Biol 177:829-41. 2007
    ..Among actin-associated proteins, Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) were predicted as PKA effectors downstream of syndecan-2, as Ena/VASP, which is activated by PKA, induces actin ..
  77. pmc alpha-Actinin links LPP, but not zyxin, to cadherin-based junctions
    Marc D H Hansen
    Physiology and Developmental Biology, Brigham Young University, 574 WIDB, Provo, UT 84602, USA
    Biochem Biophys Res Commun 371:144-8. 2008
    The actin regulator VASP localizes to cell-cell junctions and has been implicated in cell-cell adhesion. VASP is recruited to sites of actin dynamics by interactions with proline rich FPPPPP motifs...
  78. doi Estrogen ameliorates trauma-hemorrhage-induced lung injury via endothelial nitric oxide synthase-dependent activation of protein kinase G
    Wen Hong Kan
    Department of Surgery, Center for Surgical Research, University of Alabama at Birmingham, Birmingham, Alabama 35294 0019, USA
    Ann Surg 248:294-302. 2008
    ..reduces lung injury through a mechanism involving estrogen receptor (ER)-dependent activation of the endothelial nitric oxide (NO) synthase (eNOS)/protein kinase G (PKG)/vasodilator-stimulated phosphoprotein (VASP) pathway.
  79. doi Comparison of conventional aggregometry with VASP for monitoring P2Y12-specific platelet inhibition
    Daniel Aradi
    Department of Interventional Cardiology, Heart Institute, University of Pecs, Hungary
    Platelets 21:563-70. 2010
    ..Currently, the only completely P2Y12-receptor specific method is the measurement of vasodilator stimulated phosphoprotein (VASP) phosphorylation (PRI) with flow cytometry...
  80. doi Decreased VASP phosphorylation in platelets of male and female smokers of young age
    Alice Assinger
    Institute of Physiology, Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstr 17 A 1090 Vienna, Austria
    Platelets 21:596-603. 2010
    ..the impact of smoking on platelet reactivity and phosphorylation of vasodilator-stimulated phosphoprotein (VASP) in a group of 20 young smokers (age 25.7 ± 4...
  81. ncbi Comparison of platelet P2Y(12) ADP receptor-mediated pathway inhibition in triple versus dual antiplatelet therapy as assessed by VASP-phosphorylation in Japanese patients undergoing coronary stenting
    Qiang Fu
    Division of Cardiology, Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan
    Int Heart J 51:303-7. 2010
    ..Vasodilator-stimulated phosphoprotein (VASP) phosphorylation analysis and 5 and 20 µmol/L-induced maximal platelet aggregation were assessed...
  82. ncbi Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets--definition and detection of ticlopidine/clopidogrel effects
    U R Schwarz
    Institute for Clinical Biochemistry and Pathobiochemistry, Medical University Clinic, Wurzburg, Germany
    Thromb Haemost 82:1145-52. 1999
    ..In a first clinical application, the inhibitory effects of ticlopidine and clopidogrel on the platelet P2YAC ADP receptor were monitored...
  83. doi Density functional theory studies of spin, charge, and orbital ordering in YBaT2O5 (T = Mn, Fe, Co)
    R Vidya
    Center for Materials Science and Nanotechnology, Department of Chemistry, University of Oslo, Box 1033 Blindern, N 0315 Oslo, Norway
    Inorg Chem 47:6608-20. 2008
    ..The structural optimizations and BEC tensor calculations were performed using the VASP-PAW method...
  84. doi VASP is involved in cAMP-mediated Rac 1 activation in microvascular endothelial cells
    Nicolas Schlegel
    Institute of Anatomy and Cell Biology, Julius Maximilians Univ, Koellikerstr 6, 97070 Wurzburg, Germany
    Am J Physiol Cell Physiol 296:C453-62. 2009
    Accumulating evidence points to a significant role of vasodilator-stimulated phosphoprotein (VASP) in the maintenance of endothelial barrier functions...
  85. pmc c-Abl, Lamellipodin, and Ena/VASP proteins cooperate in dorsal ruffling of fibroblasts and axonal morphogenesis
    Magdalene Michael
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Curr Biol 20:783-91. 2010
    ..Lamellipodin (Lpd) regulates cell motility and recruits Ena/VASP proteins (Ena, Mena, VASP, EVL) to the leading edge of cells...
  86. doi Biochemical analysis of the human ENA/VASP-family proteins, MENA, VASP and EVL, in homologous recombination
    Motoki Takaku
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo, Japan
    J Biochem 149:721-9. 2011
    MENA, VASP and EVL are members of the ENA/VASP family of proteins and are involved in cytoplasmic actin remodeling...
  87. doi Comparison of a new ELISA assay with the flow cytometric assay for platelet vasodilator-associated stimulated phosphoprotein (VASP) phosphorylation in whole blood to assess P2Y(12) inhibition
    Joseph A Jakubowski
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Thromb Haemost 107:388-95. 2012
    ..The measurement of vasodilator-associated stimulated phosphoprotein (VASP) phosphorylation, expressed as platelet reactivity index (PRI), mirrors the degree of P2Y(12) receptor inhibition ..
  88. doi Dynamical modelling of prostaglandin signalling in platelets reveals individual receptor contributions and feedback properties
    Marcel Mischnik
    Institute of Physics, Hermann Herder Strasse 3a, 79104 Freiburg, Germany
    Mol Biosyst 9:2520-9. 2013
    ..Finally, we show here for the first time the dynamic behaviour of VASP-phosphorylation, which is commonly used as a marker for platelet-inhibition...
  89. ncbi [Feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein phosphorylation]
    Guanghua Li
    Guangdong General Hospital Guangdong Academy of Medical Science, Guangzhou 510080, China E mail
    Nan Fang Yi Ke Da Xue Xue Bao 34:434-7. 2014
    To evaluate the feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein (VASP) phosphorylation.
  90. doi Carbonic anhydrases are producers of S-nitrosothiols from inorganic nitrite and modulators of soluble guanylyl cyclase in human platelets
    Erik Hanff
    Centre of Pharmacology and Toxicology, Hannover Medical School, Carl Neuberg Str 1, 30625, Hannover, Germany
    Amino Acids 48:1695-706. 2016
    ..Upon incubation of nitrite with bovine erythrocytic CAII and washed human platelets, cGMP and P-VASP(S239) were formed in the platelets...
  91. ncbi Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome
    Brian F Volkman
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Cell 111:565-76. 2002
    Missense mutants that cause the immune disorder Wiskott-Aldrich Syndrome (WAS) map primarily to the Enabled/VASP homology 1 (EVH1) domain of the actin regulatory protein WASP...
  92. ncbi Disruption of cardiac Ena-VASP protein localization in intercalated disks causes dilated cardiomyopathy
    Martin Eigenthaler
    Institut für Klinische Biochemie und Pathobiochemie, Universitat Wurzburg, Germany
    Am J Physiol Heart Circ Physiol 285:H2471-81. 2003
    Vasodilator-stimulated phosphoprotein (VASP) and mammalian enabled (Mena) are actin cytoskeleton and signaling modulators...
  93. ncbi Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system
    Torsten Giesemann
    Cell Biology, Zoological Institute, Technical University of Braunschweig, D 38092 Braunschweig, Germany
    J Neurosci 23:8330-9. 2003
    ..I and neuronal profilin IIa, and with microfilament adaptors of the mammalian enabled (Mena)/vasodilator stimulated phosphoprotein (VASP) family, including neuronal Mena...
  94. pmc Spred-1 negatively regulates allergen-induced airway eosinophilia and hyperresponsiveness
    Hiromasa Inoue
    Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    J Exp Med 201:73-82. 2005
    ..The Sprouty-related Ena/VASP homology 1-domain-containing protein (Spred)-1 has recently been identified as a negative regulator of growth ..
  95. pmc Distinct requirements for the Sprouty domain for functional activity of Spred proteins
    James A J King
    Department of Surgery, 5th Floor Clinical Sciences Building, University of Melbourne, Royal Melbourne Hospital, Parkville, VIC 3050, Australia
    Biochem J 388:445-54. 2005
    Sprouty and Spred {Sprouty-related EVH1 [Ena/VASP (vasodilator-stimulated phosphoprotein) homology 1] domain} proteins have been identified as antagonists of growth factor signalling pathways...
  96. ncbi The NO/cGMP pathway inhibits Rap 1 activation in human platelets via cGMP-dependent protein kinase I
    Oliver Danielewski
    Institute for Biochemistry II, University of Frankfurt Medical School, Theodor Stern Kai 7, 60590 Frankfurt Main, Germany
    Thromb Haemost 93:319-25. 2005
    ..kinase (cGKI): (i) Rap 1 inhibition correlated with cGKI activity as measured by the phosphorylation state of VASP, an established substrate of cGKI, (ii) 8-pCPT-cGMP, a membrane permeable cGMP-analog and activator of cGKI, ..
  97. ncbi Activation of Rac-1 and Cdc42 stabilizes the microvascular endothelial barrier
    Jens Waschke
    Institute of Anatomy and Cell Biology, Julius Maximilians University, Koellikerstr 6, 97070, Wurzburg, Germany
    Histochem Cell Biol 125:397-406. 2006
    ..CNF-1 induced translocation of the actin-binding proteins cortactin and vasodilator-stimulated phosphoprotein (VASP) to cell junctions, increased the junction-associated actin filament belt, and reduced monolayer permeability...
  98. doi The receptor phosphatase HmLAR2 collaborates with focal adhesion proteins in filopodial tips to control growth cone morphology
    Michael W Baker
    Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Dev Biol 320:215-25. 2008
    ..RNAi of several candidate substrate signaling proteins, Lena (leech Ena/Vasp), beta-integrin and paxillin, but not beta-catenin, phenocopies particular aspects of the effects of HmLAR2 RNAi...
  99. pmc Quantification of cell edge velocities and traction forces reveals distinct motility modules during cell spreading
    Benjamin J Dubin-Thaler
    Department of Biological Sciences, Columbia University, New York, NY, USA
    PLoS ONE 3:e3735. 2008
    ..Biochemically, each motility module exhibited a different distribution of the actin-related protein VASP, while inhibition of actin polymerization revealed different dependencies on barbed-end polymerization...
  100. doi Pharmacodynamic assessment of platelet inhibition by prasugrel vs. clopidogrel in the TRITON-TIMI 38 trial
    Alan D Michelson
    Department of Medicine, Children s Hospital Boston, Center for Platelet Research Studies, Division of Hematology Oncology, Boston, MA 02115, USA
    Eur Heart J 30:1753-63. 2009
    ..To examine the extent of platelet inhibition by prasugrel vs. clopidogrel in a TRITON-TIMI 38 substudy...
  101. pmc Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines
    F J Swartling
    Department of Neurology, University of California, San Francisco, CA, USA
    Oncogene 28:3121-31. 2009
    ..All glioma cells transfected with the cGKII phosphorylate vasodilator-stimulated phosphoprotein, VASP, after cGMP analog treatment...

Research Grants62

  1. Russell E McConnell; Fiscal Year: 2014
    ..response to Epidermal Growth Factor (EGF) had increased expression levels of Mena, one of three members of the Ena/VASP family...
  2. Role of Protein Kinase D in Actin Remodeling and Cell Motiliy
    Peter Storz; Fiscal Year: 2013
    ..of the WASp family and the Arp2/3 complex, which induce actin nucleation and dendritic branching, as well as Ena/VASP, which competes with the capping protein (CP)...
  3. The role of RIAM in T cell immunity and tolerance
    Vassiliki A Boussiotis; Fiscal Year: 2013
    ..C-terminal region with multiple FPPPP motifs interacting with the EVH1 domains of the actin regulatory proteins Ena/VASP and multiple XPPPP motifs interacting with profilin...
  4. Mary C Beckerle; Fiscal Year: 2015
    ..A Stress Fiber-Remodeling, Repair, and Reinforcement (SF-R3) complex that includes zyxin, Ena-VASP proteins, and 1-actinin, has been identified...
  5. HORMONAL REGULATION OF MESSENGER RNA STABILITY
    Daniel R Schoenberg; Fiscal Year: 2013
    ..Consistent with this, PMR1 binds to the Ena/VASP proteins, which are regulators of the actin cytoskeleton, and cell motility is increased in cells ex- pressing ..
  6. Protein interactions in cardiovascular signaling
    Thomas Michel; Fiscal Year: 2009
    ..important signaling roles for caveolin in regulation of cytoskeleton-associated signaling proteins such as Rac1, VASP and MARCKS, and identified pathways for crosstalk between the platelet-derived lipid sphingosine 1-phosphate (S1P) ..
  7. Frederick Southwick; Fiscal Year: 2014
    ..after photo-bleaching (FRAP), and laser nano-scissors to assess the roles of the actin-regulatory proteins Eps8, VASP , Arp2/3 complex, fascin, and myosin-X in filopodia formation...
  8. Cytoskeletal Regulation During Growth Cone Migration and Axon Guidance
    Frank B Gertler; Fiscal Year: 2012
    ..Ena/VASP proteins function in growth cone guidance by controlling actin cytoskeleton dynamics...
  9. Fat1 and Conventional Kinesin in Lateral Polarity
    DUNCAN BRUCE JOHNSTONE; Fiscal Year: 2012
    ..on the regulation of actin dynamics by Fat1, which may occur through interaction with the actin regulatory protein VASP. During the course of this research, I will use techniques of protein biochemistry and cell biology, and I will ..
  10. Cellular and Developmental Function of Mena
    Frank B Gertler; Fiscal Year: 2012
    ..Mena, VASP and EVL, highly related proteins that comprise the Ena/VASP family, play pivotal roles in movement and shape ..
  11. MARK A PEIFER; Fiscal Year: 2016
    ..We also defined roles in morphogenesis of two key actin regulators: Ena/VASP proteins and Dia-class formins...
  12. Vijay H Shah; Fiscal Year: 2016
    ..Our exciting preliminary data demonstrates that 1) the small GTPase Rac and the actin-binding protein, VASP, promote HSC migration by generating finger-like filopodia from the plasma membrane, 2) Ableson tyrosine kinase (c- ..
  13. Identifying the Mechanisms Governing Mena-Induced Tumor Cell Dissemination
    Minna Roh; Fiscal Year: 2013
    ..of the "Invasion Signature" is Mena, a member of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family...
  14. Plasticity of the Vascular Smooth Muscle Cytoskeleton
    Kathleen G Morgan; Fiscal Year: 2011
    ..and stabilizing proteins are involved in cytoskeletal remodeling of dSMC, focusing first on profilin, N-WASP, and VASP (nucleating/elongation), and CaD, Tm, and CaP (stabilizing)...
  15. Mechanisms of Shigella intercellular spread
    MARCIA GOLDBERG; Fiscal Year: 2009
    ..will test the hypothesis that the cellular pathway of filopodia extension, which requires diaphanous formins and VASP, is used by S. flexneri to enhance its intercellular spread...
  16. David R Sherwood; Fiscal Year: 2015
    ..in selecting a single invadosome for basement membrane invasion, (3) the function of the actin regulator Ena/VASP in promoting basement membrane gap expansion at the anchor cell-basement membrane contact points after invadosome ..
  17. MUHAMMAD HAMID ZAMAN; Fiscal Year: 2016
    ..One such example is Mena, a member of the Ena/VASP family of actin regulatory proteins, which has been characterized for aberrant cell-signaling response during ..
  18. PROFILIN I--AN ESSENTIAL PROTEIN IN CELLS AND ANIMALS
    Pascal Goldschmidt Clermont; Fiscal Year: 1999
    ..one profilin binds to a cluster of 5 phospholipid molecules); (iv) profilin binds VASP (vasodilator stimulated phosphoprotein, unknown stoichiometry), possibly through its interaction wit a proline-rich stretch of the VASP ..
  19. Varicella Active Surveillance Project
    Laurene Mascola; Fiscal Year: 2010
    ..Communicable Disease Control has collaborated with CDC through its Varicella Active Surveillance Project (VASP) in Antelope Valley, CA...
  20. G Proteins and Endothelial Barrier Regulation
    Tatyana Voyno Yasenetskaya; Fiscal Year: 2004
    ..Also, G-alpha 13 may induce phosphorylation of vasodilator-stimulated phosphoprotein, VASP, which may prevent actin polymerization and thus promote the re-establishment of endothelial junctional barrier...
  21. Role of Adapter Protein in Infectious Diseases
    Maria Diakonova; Fiscal Year: 2005
    ..I have shown that SH2- Bbeta binds to VASP/profilin two proteins that have been shown to participate in actin-dependent Listeria motility...
  22. Role of TRP Channels in Statin Reversal of Nephropathy in Early Stage Type 2 DM
    STEVEN SANSOM; Fiscal Year: 2009
    ..of PKG-1a in HMC using immunostaining and Western blot and (6) the phosphorylation by 8-Br-cGMP of Ser239-VASP, a specific substrate for PKG-1a in HMC. Lay Summary...
  23. Molecular dynamics of rickettsial infection in ticks
    KEVIN MACALUSO; Fiscal Year: 2006
    ..identified as membrane receptors (clathrin-coated VATPase, E-selectin precursor, GPV-CD42D) and cytoskeletal (VASP, alpha-catenin, and alpha-tubulin) components will be assessed for their utilization by rickettsiae...
  24. Molecular Mechanisms of Portal Hypertension
    Vijay Shah; Fiscal Year: 2007
    ..Our exciting Preliminary Data demonstrates that 1) the small GTPase Rac and the actin-binding protein, VASP, promote HSC migration by generating finger-like filopodia from the plasma membrane, 2) Ableson tyrosine kinase (c-..
  25. Regulation of Osteoclast Activity by Calcium and cGMP
    Harry C Blair; Fiscal Year: 2010
    ..This will include studies of rearrangement of membrane- attachment proteins in response to NO, including VASP, migfilin and the alph-v-beta3 integrin...
  26. Arp2/3 Complex and Osteoclast Bone Resorption
    Irene Hurst; Fiscal Year: 2006
    ..regulatory protein, cortactin; third, to characterize phosphorylation of vasodilator-stimulated phosphoprotein (VASP), a regulatory protein of the Arp2/3 complex, in response to calcitonin and determine its effects on actin ring ..
  27. Mechanisms of RNA virus-host interaction
    Sailen Barik; Fiscal Year: 2009
    ..These proteins are: profilin, VASP, and zyxin. Previous studies demonstrated that these proteins were relatively non-essential for the host cell...
  28. Role of Presynaptic Proteins in Transmitter Release
    Ottavio Arancio; Fiscal Year: 2004
    ..The goal of the second specific aim will be to test whether VASP and CAMKII, two cGK substrates, are cGK targets during neurotransmission and plasticity...
  29. Surface-bound Regulation of Actin Polymerization
    SCOT KUO; Fiscal Year: 2009
    ..By examining ARP2/3 and Ena/VASP-dependent reactions, we will probe the physicochemical constraints of membrane-surface catalysis in two modes of ..
  30. STRUCTURAL BIOLOGY OF CYTOSKELETAL DYNAMICS
    STEVEN ALMO; Fiscal Year: 2003
    ..Scaffolding proteins of the Ena/VASP/WASP family participate in the formation of multicomponent assemblies that direct the site specific, de novo ..
  31. ATP in Lung Endothelial Barrier Enhancement
    Alexander Verin; Fiscal Year: 2009
    ..inhibition attenuates both ATP-induced increases in TER and phosphorylation of vasolidator- stimulated protein (VASP), which in the phosphorylated form inhibits stress fiber formation supporting the involvement of the PKA/VASP ..
  32. STRUCTURE AND FUNCTION OF VASCULAR INTEGRINS
    LESLIE PARISE; Fiscal Year: 2007
    ..The role of VASP in actin polymerization and activation of integrins will be determined...
  33. Tyrosine Kinases in Autoimmunity
    CONSTANTINE TSOUKAS; Fiscal Year: 2007
    ..g. VASP and Arp2/3), and the Ilk structural requirements for these interactions, the role of the Itk-mediated ..
  34. Analysis of Ena/VASP Anti-Capping Activity
    Craig Furman; Fiscal Year: 2005
    ..Ena/VASP proteins are key regulators of cell migration...
  35. Enabled, a regulator of adhesion and cell motility
    Julie Gates; Fiscal Year: 2005
    ..Recent studies have revealed that members of the Ena/VASP protein family not only promote cell adhesion, but also inhibit cell motility by regulating localized actin ..
  36. The role of Ena/VASP proteins in muscle development
    KATHERINE KRAGTORP; Fiscal Year: 2006
    The role of Ena/VASP proteins in the organization of the actin cytoskeleton and the regulation of cell motility and axon outgrowth has been well established...
  37. The Role of Rap1 in T-Cell Responses
    Vassiliki Boussiotis; Fiscal Year: 2003
    ..RIAM provides a link between Rapl-GTP and the cytoskeleton by interacting with Mena, VASP and WASP and the actin regulator profilin...
  38. Cytoskeletal Responses to Guidance Signaling
    ELAINE PINHEIRO; Fiscal Year: 2007
    ..How this transduction of signaling to actin dynamics occurs is largely unknown. The Ena/VASP proteins are known actin regulators implicated in axon guidance...
  39. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Keith Johnson; Fiscal Year: 2009
    ..1) to determine if alpha-catenin interacts directly with components of invadopodia;2) to test the hypotheses that VASP function is important for maintenance of invadopodia and that expression of beta-catenin modulates VASP function;..
  40. p190 RhoGAP Regulation and Function
    Jennifer Lamb; Fiscal Year: 2007
    Actin remodeling at the plasma membrane is required for cell migration. The Ena/VASP family of actin regulatory proteins are key regulators of filopodia and lamellipodia dynamics, both environment sensing protrusions involved in migration...
  41. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    DEREK APPLEWHITE; Fiscal Year: 2006
    ..transition that bind to the barbed-end of actin filaments, the heterodimeric capping protein (CP) and the Ena/VASP family of molecules...
  42. ACTIVE-BASED MOTILITY BY CLAMPED-FILAMENT MOTORS
    DANIEL PURICH; Fiscal Year: 2006
    ..anisotropy measurements to learn if and how ATP hydrolysis modulates the strength of binding interactions of VASP's clamping domain with the ends of actin filaments...
  43. VASP: Regulator of Vascular Smooth Muscle Cell Function
    Alexander Clowes; Fiscal Year: 2008
    Vasodilator-stimulated phosphoprotein (VASP) is a focal adhesion protein, a downstream target of nitric oxide, and a substrate for cyclic GMP-dependent kinase (PKG) and cyclic AMP-dependent kinase (PKA)...
  44. PKA and PAK in Adhesion Dependent Signal Transduction
    Alan Howe; Fiscal Year: 2005
    ....
  45. ATOMIC STRUCTURE OF SMOOTH MUSCLE CALDESMON
    Roberto Dominguez; Fiscal Year: 2004
    ....
  46. Molecular determinants of cellular trajectory/motility
    CATHERINE LACAYO; Fiscal Year: 2006
    ..Characterize directional motility in neutrophils and begin analysis of molecular contributions by VASP (Vasodilator-stimulated phosphoprotein).
  47. Spatial regulation of Protein Kinase A in cell migration
    Alan K Howe; Fiscal Year: 2010
    ..abstract_text> ..
  48. Structure of the Smooth Muscle Myosin Phosphatase
    Roberto Dominguez; Fiscal Year: 2008
    ..This knowledge will prove invaluable for the development of new drugs to treat cardiovascular diseases such as hypertension. ..
  49. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  50. A biochemical screen for novel regulators of neuronal morphogenesis
    ANTHONY KOLESKE; Fiscal Year: 2007
    ..Identifying these substrate will expand the "toolkit" available to dissect the molecular mechanisms of axon and dendrite morphogenesis. [unreadable] [unreadable]..
  51. The PINCH-ILK-parvin complexes in glomerular cells
    Chuanyue Wu; Fiscal Year: 2010
    ..The proposed studies, therefore, not only will advance our understanding of the molecular mechanisms that govern glomerular cell behavior but also may lead to novel therapeutic approaches that alleviate progressive glomerular failure. ..
  52. Ceruloplasmin Mediated Penile Endothelial Dysfunction in Type 1 Diabetes
    Kanchan Chitaley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  53. Shared Zeiss 510META Confocal Microscope
    Elaine Bearer; Fiscal Year: 2008
    ..Their research applications address fundamental questions regarding prevalent disease with important impacts on public health. [unreadable] [unreadable] [unreadable]..
  54. Light Adaptation in Cone Photoreceptors
    FREDERICK SOO; Fiscal Year: 2007
    ..A suction electrode technique is used throughout, which allows recording from individual, intact photoreceptors. ..
  55. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
    ....
  56. REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING
    Michael S Wolin; Fiscal Year: 2010
    ..These studies should help define the origins of differences in physiological responses of pulmonary and coronary arteries to changes in O2 tension. ..
  57. Molecular mechanisms of anterograde transport
    Elaine Bearer; Fiscal Year: 2007
    ..Thus, these results will yield answers to universal questions on axonal transport, specific facts about herpes pathogenesis, and fundamental new information about the role of APP in Alzheimer's disease. [unreadable] [unreadable]..
  58. Biological Role of the FE65 Protein/APP Interactions
    Suzanne Guenette; Fiscal Year: 2009
    ..In addition, we will gain insight into the role of the FE65 proteins in cellular signaling events that are critical for brain function. ..
  59. Erectile Dysfunction in Type II Diabetes: Role of Adiponectin
    Kanchan Chitaley; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  60. MOLECULAR MECHANISMS OF ACTIN DYNAMICS IN PLATELETS
    Elaine Bearer; Fiscal Year: 2003
    ..Two other proteins, Arp2 and VASP also induce actin polymerization...
  61. Conference on Wiring and Plasticity of Nervous Systems
    Thomas Pollard; Fiscal Year: 2002
    ..We are confident that mutually beneficial insights will emerge from learning about each others work. ..