Genomes and Genes
Gene Symbol: tryptase
Description: tryptase alpha/beta 1
Alias: TPS1, TPS2, TPSB1, tryptase alpha/beta-1, mast cell alpha II tryptase, mast cell beta I tryptase, tryptase alpha II, tryptase alpha-1, tryptase beta I, tryptase beta-1, tryptase-1, tryptase-I, tryptase-III
Publications203 found, 100 shown here
- Mast cell tryptase and hemolysis after traumaErik Edston
Department of Forensic Medicine, University Hospital, S 581 85 Linkoping, Sweden
Forensic Sci Int 131:8-13. 2003We have previously found increased mast cell tryptase in accidental deaths due to trauma, indicating that mast cell degranulation had occurred...
- Tryptase activates peripheral blood mononuclear cells causing the synthesis and release of TNF-alpha, IL-6 and IL-1 beta: possible relevance to multiple sclerosisVivian Malamud
Department of Cellular Biochemistry and Human Genetics, Hebrew University, Hadassah Medical School, P O Box 12272, 91120 Jerusalem, Israel
J Neuroimmunol 138:115-22. 2003..The most abundant secretory mediator of the human mast cell is the tetrameric protease tryptase. We demonstrate that tryptase can activate peripheral mononuclear cells (PBMCs), isolated from healthy donors as ..
- Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease familyP Vanderslice
Cardiovascular Research Institute, University of California, San Francisco 94143
Proc Natl Acad Sci U S A 87:3811-5. 1990Three different cDNAs and a gene encoding human skin mast cell tryptase have been cloned and sequenced in their entirety. The deduced amino acid sequences reveal a 30-amino acid prepropeptide followed by a 245-amino acid catalytic domain...
- Cloning and characterization of a second complementary DNA for human tryptaseJ S Miller
Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298
J Clin Invest 86:864-70. 1990A second cDNA for human tryptase, called beta-tryptase, was cloned from a mast cell cDNA library in lambda ZAP...
- Cloning and characterization of complementary DNA for human tryptaseJ S Miller
Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298
J Clin Invest 84:1188-95. 1989The amino acid sequence of human mast cell tryptase was determined from corresponding cDNA cloned from a lambda ZAP library made with mRNA derived from a human mast cell preparation...
- The fibrinogenolytic activity of purified tryptase from human lung mast cellsL B Schwartz
J Immunol 135:2762-7. 1985The capacity of purified tryptase from human lung mast cells to metabolize human fibrinogen, fibrin, and plasminogen was evaluated...
- Characterization of a tryptase mRNA expressed in the human basophil cell line KU812T Blom
Department of Immunology, Biomedicum, University of Uppsala, Sweden
Scand J Immunol 37:203-8. 1993..PCR primers designed for the amplification of a nearly full-length copy of tryptase mRNAs were used to study the identity of the KU812 tryptase...
- Mast cell tryptase is a mitogen for epithelial cells. Stimulation of IL-8 production and intercellular adhesion molecule-1 expressionJ A Cairns
Immunopharmacology Group, Southampton General Hospital, UK
J Immunol 156:275-83. 1996b>Tryptase, a protease unique to the mast cell secretory granule, is released in substantial quantities into the respiratory tract of patients with inflammatory disease of the airways...
- The alpha form of human tryptase is the predominant type present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosisL B Schwartz
Department of Medicine, Virginia Commonwealth University, Richmond 23298, USA
J Clin Invest 96:2702-10. 1995b>Tryptase, a protease produced by all mast cells, was evaluated as a clinical marker of systemic mastocytosis. Two sandwich immunoassays were evaluated, one which used the mAb G5 for capture, the other which used B12 for capture...
- Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblastsJ A Cairns
Immunopharmacology Group, Southampton General Hospital, United Kingdom
J Clin Invest 99:1313-21. 1997..We have investigated the potential of tryptase, the major protease of human mast cells, to stimulate collagen synthesis in the human lung fibroblast cell line ..
- Human mast cell tryptase fibrinogenolysis: kinetics, anticoagulation mechanism, and cell adhesion disruptionV A Thomas
Department of Biochemistry and Molecular Biology, J H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0581, USA
Biochemistry 37:2291-8. 1998b>Tryptase is a 31 kDa, glycosylated, trypsin-like enzyme stored in and released from mast cell granules...
- Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3M Pallaoro
Cardiovascular Research Institute and Departments of Medicine and Laboratory Medicine, University of California, San Francisco, California 94143 0911, USA
J Biol Chem 274:3355-62. 1999..They fall into two groups, alpha and beta. Although several related tryptase mRNAs are known, it is unclear which if any are transcripts of separate haploid genes...
- Human tryptases alpha and beta/II are functionally distinct due, in part, to a single amino acid difference in one of the surface loops that forms the substrate-binding cleftC Huang
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 274:19670-6. 1999..Structural modeling analyses revealed that tryptase alpha differs from tryptase beta/II in that it possesses an Asp, rather than a Gly, in one of the loops that form ..
- Characterization of human gamma-tryptases, novel members of the chromosome 16p mast cell tryptase and prostasin gene familiesG H Caughey
Cardiovascular Research Institute and Department of Medicine, University of California, San Francisco 94143, USA
J Immunol 164:6566-75. 2000Previously, this laboratory identified clusters of alpha-, beta-, and mast cell protease-7-like tryptase genes on human chromosome 16p13.3...
- Characterization of two highly polymorphic human tryptase loci and comparison with a newly discovered monkey tryptase orthologM Guida
PPGx Incorporated, La Jolla, CA 92037, USA
Pharmacogenetics 10:389-96. 2000..data on the tryptase inhibitors undergoing clinical trials, we defined the genetic variability of the tryptase 1 (TPS1) and tryptase 2 (TPS2) loci by screening a reference population of 32 individuals representing three major ..
- Genetic deficiency of human mast cell alpha-tryptaseD Soto
Cardiovascular Research Institute and Department of Medicine, University of California at San Francisco, San Francisco, California 94143 0911, USA
Clin Exp Allergy 32:1000-6. 2002..Human alpha- and beta-tryptases are proteases secreted by mast cells. Beta (but not alpha) tryptases are implicated in asthma. Genes encoding both types of tryptases cluster on chromosome 16p13.3...
- Tryptase haplotype in mastocytosis: relationship to disease variant and diagnostic utility of total tryptase levelsCem Akin
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA
Clin Immunol 123:268-71. 2007Serum mast cell tryptase levels are used as a diagnostic criterion and surrogate marker of disease severity in mastocytosis...
- Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formationNicole E Jackson
Centre for Infection and Inflammation Research, School of Medical Sciences, Sydney, New South Wales 2052, Australia
J Biol Chem 283:34178-87. 2008..This model explains why many of the preferred substrates of tryptase are short peptides; however, it does not explain how tryptase cleaves large protein substrates such as ..
- Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutationsNeil N Trivedi
Cardiovascular Research Institute, University of California at San Francisco, San Francisco, Calif, USA
J Allergy Clin Immunol 124:1099-105.e1-4. 2009Mast cell tryptases have proposed roles in allergic inflammation and host defense against infection. Tryptase gene loci TPSAB1 and TPSB2 are known to be polymorphic, but the nature and extent of diversity at these loci have not been fully ..
- Serum total tryptase levels are increased in patients with active chronic urticariaM Ferrer
Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra Medical School, Pamplona, Spain
Clin Exp Allergy 40:1760-6. 2010..We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation...
- Mast cell tryptase deficiency attenuates mouse abdominal aortic aneurysm formationJie Zhang
Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Circ Res 108:1316-27. 2011..b>Tryptase is the most abundant MC granule protein and participates in MC activation, protease maturation, leukocyte ..
- Tissue remodelling in breast cancer: human mast cell tryptase as an initiator of myofibroblast differentiationAnita Mangia
Clinical Experimental Oncology Laboratory, National Cancer Centre Giovanni Paolo II, University of Bari, Bari, Italy
Histopathology 58:1096-106. 2011..We investigated α-smooth-muscle actin (α-SMA(+) ) and CD34(+) fibroblasts, density of toluidine blue-stained (MCs-TB) and tryptase-immunolabelled MCs (MCs-Try) in 30 primary breast tumours.
- Immunoglobulin E and mast cell proteases are potential risk factors of human pre-diabetes and diabetes mellitusZhen Wang
Department of Clinical Medicine, School of Medicine, Huzhou Teachers College, Huzhou, Zhejiang, China
PLoS ONE 6:e28962. 2011..Plasma levels of mast cell proteases and the mast cell activator immunoglobulin E (IgE) may serve as novel inflammatory markers that associate with the risk of pre-diabetes and diabetes mellitus...
- Immunoreactivity for CD25 in gastrointestinal mucosal mast cells is specific for systemic mastocytosisHejin P Hahn
Department of Pathology, Brigham and Women s Hospital, and Harvard Medical School, Boston, MA 02115, USA
Am J Surg Pathol 31:1669-76. 2007..infections, and 9 biopsies from 7 patients with eosinophilic gastroenteritis were immunostained for mast cell tryptase, c-kit (CD117), and CD25...
- Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cellsXue Qiong Zhu
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
Gynecol Oncol 105:650-6. 2007..The purpose of this study was to reveal the different mast cell subsets in smooth muscle tumors of uterus and to investigate the mechanism of local increase of mast cells...
- Mastocytic enterocolitis: increased mucosal mast cells in chronic intractable diarrheaShriram Jakate
Department of Pathology, Rush University Medical Center, Chicago, IL 60612, USA
Arch Pathol Lab Med 130:362-7. 2006..In some adult patients with chronic intractable diarrhea, the diagnosis remains elusive even after detailed evaluations, and colonic or duodenal biopsy specimens may appear unremarkable on routine hematoxylin-eosin staining...
- Mast cells cultured from the peripheral blood of normal donors and patients with mastocytosis originate from a CD34+/Fc epsilon RI- cell populationM Rottem
Allergic Diseases Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
Blood 84:2489-96. 1994..cell number and cell differential using Wright Giemsa and acid toluidine blue stains and antibodies to mast cell tryptase and chymase, cell-associated histamine, and expression of CD34, c-kit, Fc epsilon RI, and Fc gamma RII using flow ..
- Eosinophilic gastroenterocolitis in iron lactate-overloaded ratsI Narama
Research Institute of Drug Safety, Setsunan University, Osaka, Japan
Toxicol Pathol 27:318-24. 1999..The morphologic characteristics of the gastrointestinal lesions and peripheral eosinophilia induced in rats fed iron lactate were very similar to those in some cases of eosinophilic gastroenterocolitis in humans and other animals...
- Increased mast cells and neutrophils in submucosal mucous glands and mucus plugging in patients with asthmaN G Carroll
Faculty of Regional Professional Studies, Edith Cowan University, Bunbury 6230, Australia
Thorax 57:677-82. 2002..A study was undertaken to count the mast cells and neutrophils in submucosal glands and to relate cell numbers to the presence of mucus in the airway lumen...
- Proteinase-activated receptor (PAR)-1 and -2 agonists induce mediator release from mast cells by pathways distinct from PAR-1 and PAR-2Grant R Stenton
Pulmonary Research Group, Department of Medicine, Astra Zeneca Canada Inc, Glaxo Heritage Asthma Research Laboratory, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
J Pharmacol Exp Ther 302:466-74. 2002..from peritoneal mast cells, whereas another PAR-2AP (SLIGRL-NH(2), 10 microM), trypsin (40 U/ml), and mast cell tryptase (1.5 microg/ml) did not. PAR-1AP (ApfFRChaCitY-NH(2), 10 microM) (Cit) induced 11.7 +/- 3...
- Association of mast cells with calcification in the human pineal glandDanuta Maslinska
Department of Experimental and Clinical Neuropathology, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego, Warsaw
Folia Neuropathol 48:276-82. 2010..stained with haematoxylin and eosin or the von Kossa staining technique and for the distribution of mast cell tryptase, mast cell chymase, histamine H4 receptor and vascular network using biotinylated Ulex europaeus agglutinin...
- Vasoactive intestinal peptide (VIP) treatment of Parkinsonian rats increases thalamic gamma-aminobutyric acid (GABA) levels and alters the release of nerve growth factor (NGF) by mast cellsOrhan Tansel Korkmaz
Department of Physiology, Medical Faculty, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey
J Mol Neurosci 41:278-87. 2010..Therefore, the protective effect of VIP on motor function is possibly related to the increased levels of GABA in the VATh, and its neuroprotective actions may be mediated by the release of NGF from brain mast cells...
- A sensitive colorimetric assay for the release of tryptase from human lung mast cells in vitroS E Lavens
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, UK
J Immunol Methods 166:93-102. 1993..We have used a simple colorimetric assay for tryptase to follow the release of proteolytic enzymes from human lung mast cells in vitro...
- Activated mast cells in proximity to colonic nerves correlate with abdominal pain in irritable bowel syndromeGiovanni Barbara
Departmentof Internal Medicine and Gastroenterology, and CRBA, University of Bologna, Italy
Gastroenterology 126:693-702. 2004..We assessed colonic mast cell infiltration, mediator release, and spatial interactions with mucosal innervation and their correlation with abdominal pain in IBS patients...
- Expression of mast cell proteases in rat lung during helminth infection: mast cells express both rat mast cell protease II and tryptase in helminth infected lungM Tomita
Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki, Japan
Int Arch Allergy Immunol 120:303-9. 1999..However, a previous study of ours showed that the expression of rat mast cell tryptase (RMCT) mRNA, which has been reported to be confined to CTMC, significantly increased in rat lung 14 days after ..
- Mast cells: the forgotten cells of renal fibrosisI S Roberts
Department of Cellular Pathology, Level 1, John Radcliffe Hospital, Headley Way, Headington, Oxford OX3 9DU, UK
J Clin Pathol 53:858-62. 2000..Therefore, the numbers of mast cells in normal and fibrotic kidneys with various pathologies were investigated...
- Contribution of mast cells to the tubulointerstitial lesions in IgA nephritisT Ehara
First Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
Kidney Int 54:1675-83. 1998..Mast cells have been found to secrete basic fibroblast growth factor (bFGF) in human pulmonary fibrosis...
- Inhibition of keratinocyte growth in cell culture and whole skin culture by mast cell mediatorsM Huttunen
Department of Dermatology, Kuopio University Hospital, Kuopio, Finland
Exp Dermatol 10:184-92. 2001..in a 24-well plate, and the cells were treated with different mast cell mediators histamine, heparin and tryptase, or lysate from HMC-1 cells, a human leukemic mast cell line...
- A pilot study of fecal serine-protease activity: a pathophysiologic factor in diarrhea-predominant irritable bowel syndromeRichard Roka
Institut National de la Recherche Agronomique, Neuro Gastroenterology and Nutrition Unit, Toulouse, France
Clin Gastroenterol Hepatol 5:550-5. 2007..Our study was designed to evaluate if colonic luminal serine-proteases may be a relevant pathophysiologic marker of IBS...
- Protease-activated receptor 2 expression in trigeminal neurons innervating the rat nasal mucosaQ Thai Dinh
Department of Internal Medicine, Charite School of Medicine, Humboldt University, Augustenburger Platz 1, 13353 Berlin, Germany
Neuropeptides 39:461-6. 2005Protease-activated receptor 2 (PAR2) is activated by trypsin and mast cell tryptase to induce widespread inflammation by unknown mechanisms...
- Human mast cells express functional TrkA and are a source of nerve growth factorG Nilsson
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA
Eur J Immunol 27:2295-301. 1997..Using flow cytometric analysis and mast cell tryptase as a mast cell marker, both HMC-1 cells and cultured human mast cells were shown to coexpress tryptase and TrkA...
- Cathepsin B activates human trypsinogen 1 but not proelastase 2 or procarboxypeptidase BBjorn Lindkvist
Department of Surgery, Malmo University Hospital, Lund University, Malmo, Sweden
Pancreatology 6:224-31. 2006..not known although suggested explanations include autoactivation, cathepsin B-mediated activation and activation by mast cell tryptase. The aim of this study was to investigate cathepsin B and tryptase activation of pancreatic zymogens.
- Mast cell tryptase causes homologous desensitization of beta-adrenoceptors by Ca2+ sensitization in tracheal smooth muscleM Kobayashi
Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
Clin Exp Allergy 38:135-44. 2008Recent studies have revealed that in asthma, mast cells infiltrate to the smooth muscle layer and release tryptase, an enzymatic activator of protease-activated receptor 2 (PAR2)...
- Lipid-rich enteral nutrition reduces postoperative ileus in rats via activation of cholecystokinin-receptorsTim Lubbers
Department of Surgery, Nutrition and Toxicology Research Institute Maastricht, Maastricht University Medical Centre, Maastricht, The Netherlands
Ann Surg 249:481-7. 2009..This study investigates the effect of lipid-rich nutrition on the local inflammatory response and gastrointestinal hypomotility in a rat model of postoperative ileus...
- Protease-activated receptor-2 mediates proliferative responses in skeletal myoblastsC Chinni
School of Veterinary Science, University of Melbourne, Parkville, Victoria 3010, Australia
J Cell Sci 113:4427-33. 2000..Activators of PAR-2 include trypsin and mast cell tryptase. Skeletal myoblasts are known to express PAR-1, a thrombin receptor...
- Localized Th1-, Th2-, T regulatory cell-, and inflammation-associated hepatic and pulmonary immune responses in Ascaris suum-infected swine are increased by retinoic acidHarry Dawson
Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, U S Department of Agriculture, Beltsville, MD 20705, USA
Infect Immun 77:2576-87. 2009..T regulatory (Treg) cells, while infected pigs given ATRA had higher IL4, IL13, CCL11, CCL26, CCL17, CCL22, and TPSB1 expression...
- Expulsion of secondary Trichinella spiralis infection in rats occurs independently of mucosal mast cell release of mast cell protease IILisa K Blum
James A Baker Institute for Animal Health, Cornell University, Ithaca, NY 14853, USA
J Immunol 183:5816-22. 2009..Furthermore, release of RMCPII is not sufficient to cause expulsion. The data argue against a role for mast cells in the mechanism underlying the effector phase of protective immunity against T. spiralis in rats...
- Mast cell tryptase in postmortem serum-reference values and confoundersErik Edston
Institution for Clinical and Molecular Medicine, Linkoping University, Linköping 581 85, Sweden
Int J Legal Med 121:275-80. 2007We have investigated the effects of some factors suspected of inducing spuriously increased tryptase concentrations, specifically sampling site, conjunctival petechial bleeding and prone position at the time of death as indicators of ..
- Local production of total IgE and specific antibodies to the house dust mite in adenoid tissueSeung Youp Shin
Department of Otorhinolaryngology, College of Medicine, Kyunghee University, Seoul, Korea
Pediatr Allergy Immunol 20:134-41. 2009..homogenate were performed to quantify the levels of total IgE, eosinophil cationic protein (ECP), and mast cell tryptase. DP-specific IgE, IgA, IgG1, and IgG4 antibodies, soluble IL-2 receptors (sIL-2R), soluble CD23 (sCD23), and IL-6 ..
- A comparative study of the spatial distribution of mast cells and microvessels in the foetal, adult human thymus and thymomaMarius Raica
Department of Histology and Cytology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
Int J Exp Pathol 91:17-23. 2010..Double staining with CD34 and mast cell tryptase was used to count both mast cells and microvessels in the same fields...
- Genomic organization and chromosomal localization of the human cathepsin G geneP A Hohn
Department of Medicine, Jewish Hospital, Washington University Medical Center, St Louis, Missouri 63110
J Biol Chem 264:13412-9. 1989..Having defined the genomic structure and chromosomal location of cathepsin G, we are now attempting to identify the DNA elements in or near this gene that mediate its tissue and development-specific pattern of expression...
- The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-beta-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damageChristiane Schnee
Max Planck Institute for Chemical Ecology, Winzerlaer Strasse 10, D 07745 Jena, Germany
Plant Physiol 130:2049-60. 2002..We isolated a terpene synthase gene, terpene synthase 1 (tps1), from maize that exhibits only a low degree of sequence identity to previously identified terpene synthases...
- Three trehalose synthetic pathways in the acarbose-producing Actinoplanes sp. SN223/29 and evidence for the TreY role in biosynthesis of component CJin Sook Lee
Institute of Molecular Microbiology and Biotechnology, Westfällische Wilhelms Universität Münster, Munster, Germany
Appl Microbiol Biotechnol 80:767-78. 2008In this study, three trehalose gene clusters, treX-Y-Z, tpS1, and treS, of the acarbose-producing strain, Actinoplanes sp. SN223/29, have been identified...
- Delayed embryo development in the ARABIDOPSIS TREHALOSE-6-PHOSPHATE SYNTHASE 1 mutant is associated with altered cell wall structure, decreased cell division and starch accumulationLeonardo D Gomez
CNAP, Department of Biology, University of York, Heslington, York YO10 5YW, UK
Plant J 46:69-84. 2006The tps1 mutant, which is disrupted in the TREHALOSE-6-PHOSPHATE SYNTHASE 1 gene, has been previously characterized as a recessive embryo lethal. tps1 embryos do not develop past late torpedo or early cotyledon stage...
- Novel alleles of yeast hexokinase PII with distinct effects on catalytic activity and catabolite repression of SUC2S Hohmann
Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Heverlee, Flanders, Belgium
Microbiology 145:703-14. 1999..Mutants lacking the TPS1 gene, which encodes trehalose 6-phosphate synthase, can not grow on glucose or fructose...
- Gene and protein expression of protease-activated receptor 2 in structural and inflammatory cells in the nasal mucosa in seasonal allergic rhinitisQ T Dinh
Department of Internal Medicine, Charite Universitatsmedizin Berlin, Berlin, Germany
Clin Exp Allergy 36:1039-48. 2006Protease-activated receptor 2 (PAR 2) has been shown to be responsible for trypsin and mast cell tryptase-induced airway inflammation...
- The trehalose synthesis pathway is an integral part of the virulence composite for Cryptococcus gattiiPopchai Ngamskulrungroj
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
Infect Immun 77:4584-96. 2009..Three genes putatively involved in trehalose biosynthesis, TPS1 (trehalose-6-phosphate [T6P] synthase) and TPS2 (T6P phosphatase), and degradation, NTH1 (neutral trehalose), were ..
- [The molecular composition and spectral properties of polysaccharide isolated from pu-erh tea and its material]Jia Shun Gong
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China
Guang Pu Xue Yu Guang Pu Fen Xi 30:1960-4. 2010..The main polysaccharide TPS2 and TPS1 were isolated and purified from pu-erh tea and its materials by DEAE-52 and Sephadex G-150 column ..
- Glycoconjugate expression on the cell wall of tps1/tps1 trehalose-deficient Candida albicans strain and implications for its interaction with macrophagesMaría Martínez-Esparza
Facultad de Medicina, Departamento de Bioquimica, Biología Molecular B e Inmunología, Universidad de Murcia, Murcia, Spain
Glycobiology 21:796-805. 2011..The gene-encoding trehalose-6-phosphate synthase (TPS1), which is responsible for trehalose synthesis, is induced in response to oxidative stress, as in phagolysosomes...
- Interactions of mast cell tryptase with thrombin receptors and PAR-2M Molino
Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 272:4043-9. 1997b>Tryptase is a serine protease secreted by mast cells that is able to activate other cells...
- Eosinophils are activated in middle ear mucosa and middle ear effusion of patients with intractable otitis media associated with bronchial asthmaY Iino
Department of Otolaryngology, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi ku, Tokyo 173 8605, Japan
Clin Exp Allergy 31:1135-43. 2001....
- Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitisAkira Matsuda
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
Invest Ophthalmol Vis Sci 50:2871-7. 2009....
- The orlA gene from Aspergillus nidulans encodes a trehalose-6-phosphate phosphatase necessary for normal growth and chitin synthesis at elevated temperaturesP T Borgia
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 1120, USA
Mol Microbiol 20:1287-96. 1996..Da) that is homologous to a 211-amino-acid domain from the polypeptide encoded by the Saccharomyces cerevisiae TPS2 gene and to almost the entire Escherichia coli otsB-encoded polypeptide...
- Dietary wheat germ agglutinin modulates ovalbumin-induced immune responses in Brown Norway ratsB Watzl
Institute of Nutritional Physiology, Institute of Hygiene and Toxicology, Federal Research Centre for Nutrition, Haid und Neu Str, 9, 76131 Karlsruhe, Germany
Br J Nutr 85:483-90. 2001..In conclusion, these data suggest that high dietary intake of lectins such as WGA may affect the allergic response towards oral antigens in the gut-associated lymphoid tissue...
- Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulansS Fillinger
Unité Microbiologie et Environnement, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
Microbiology 147:1851-62. 2001..Expression of tpsA in a Saccharomyces cerevisiae tps1 mutant revealed that the tpsA gene product is a functional equivalent of the yeast Tps1 trehalose-6-phosphate ..
- Basic fibroblast growth factor and its receptors in idiopathic pulmonary fibrosis and lymphangioleiomyomatosisYoshikazu Inoue
Division of Environmental and Occupational Health Sciences and Pulmonary Division, Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado, USA
Am J Respir Crit Care Med 166:765-73. 2002..We performed quantitative immunohistochemistry for bFGF, mast cell tryptase, smooth muscle actin for smooth muscle cell/myofibroblast-like cells, and fibroblast growth factor receptors (Flg,..
- Systemic mastocytosis involving the gastrointestinal tract: clinicopathologic and molecular study of five casesRichard Kirsch
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Toronto, ON, Canada
Mod Pathol 21:1508-16. 2008..Histologic features were documented in slides stained with hematoxylin and eosin, mast cell tryptase and CD117...
- Truncation of Arabidopsis thaliana and Selaginella lepidophylla trehalose-6-phosphate synthase unlocks high catalytic activity and supports high trehalose levels on expression in yeastPatrick Van Dijck
Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven and Vlaams Interuniversitair Instituut voor Biotechnologie VIB, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Flanders, Belgium
Biochem J 366:63-71. 2002..lepidophylla, being a notable exception. Overexpression of the plant genes in a Saccharomyces cerevisiae tps1 mutant results in very low TPS-catalytic activity and trehalose accumulation...
- Stem cell factor in a rat model of serum nephrotoxic nephritisM M El Kossi
Sheffield Kidney Institute, University of Sheffield, Sheffield, UK
Nephron Exp Nephrol 108:e1-e10. 2008..The growth factor (SCF) was evaluated in a rat model of nephrotoxic serum nephritis (NTN), characterized by early inflammation followed by later tissue fibrosis...
- Mast cell burden and reticulin fibrosis in the myeloproliferative neoplasms: a computer-assisted image analysis studyArsalan Ahmed
The Department of Pathology and Laboratory Medicine, The Methodist Hospital and Research Institute, Houston, TX 77030, USA
Pathol Res Pract 205:634-8. 2009..We used computer-assisted image analysis of bone marrow core biopsies stained for mast cell tryptase from patients with myeloproliferative neoplasms [31 cases: 12 chronic myelogenous leukemia (CML), 6 primary ..
- Evidence for the involvement of free light chain immunoglobulins in allergic and nonallergic rhinitisDesmond G Powe
Department of Histopathology, Surgical and Medical Sciences, Queen s Medical Centre, Nottingham University Hospitals Trust, Nottingham NG7 2UH, United Kingdom
J Allergy Clin Immunol 125:139-45.e1-3. 2010..Recent murine studies suggest the existence of an IgE-independent hypersensitivity response involving antigen-induced mast cell activation, mediated by immunoglobulin free light chains (FLCs)...
- Lymphocyte sub-populations and non-Langerhans' cell monocytoid cells in pulmonary Langerhans' cell histiocytosisR Nagarjun Rao
Department of Pathology, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
Pathol Res Pract 204:315-22. 2008..for lymphohistiocytic markers: CD1a, CD3, CD4, CD8, CD15, CD20, CD56, TIA-1, CD68-PGM1, Mac387, and mast cell tryptase. Double immunolabeling was performed with CD1a/Mac387...
- Prophylaxis with ketotifen in rats with portal hypertension: involvement of mast cell and eicosanoidsFernando Sanchez-Patan
Department of Surgery I, School of Medicine, Complutense University of Madrid, Spain
Hepatobiliary Pancreat Dis Int 7:383-94. 2008....
- Mast cell tryptase from pig lungs triggers infection by pneumotropic Sendai and influenza A viruses. Purification and characterizationY Chen
Division of Enzyme Chemistry, Institute for Enzyme Research, University of Tokushima, Japan
Eur J Biochem 267:3189-97. 2000..Heparin stabilized the enzyme, but high-ionic-strength conditions did not, unlike for human mast cell tryptase. The purified enzyme efficiently processed the fusion glycoprotein precursor of Sendai virus and slowly processed ..
- Serum tryptase monitoring in indolent systemic mastocytosis: association with disease features and patient outcomeAlmudena Matito
Instituto de Estudios de Mastocitosis de Castilla La Mancha, Hospital Virgen del Valle, Toledo, Spain Spanish Network on Mastocytosis REMA
PLoS ONE 8:e76116. 2013Serum baseline tryptase (sBT) is a minor diagnostic criterion for systemic mastocytosis (SM) of undetermined prognostic impact...
- Characterizing roles for the glutathione reductase, thioredoxin reductase and thioredoxin peroxidase-encoding genes of Magnaporthe oryzae during rice blast diseaseJessie Fernandez
Department of Plant Pathology, University of Nebraska Lincoln, Lincoln, Nebraska, United States of America
PLoS ONE 9:e87300. 2014..oryzae sugar/ NADPH sensor Tps1, thereby suggesting how glucose availability, NADPH production and antioxidation might be connected...
- Mode of action of the qcr9 and cat3 mutations in restoring the ability of Saccharomyces cerevisiae tps1 mutants to grow on glucoseM A Blazquez
Unidad de Bioquímica y Genética de Levaduras, Instituto de Investigaciones Biomédicas del C S I C, Madrid, Spain
Mol Gen Genet 249:655-64. 1995Mutations in the TPS1 gene, which encodes trehalose-6-P synthase, cause a glucose-negative phenotype in Saccharomyces cerevisiae...
- Sphingolipids are potential heat stress signals in SaccharomycesR C Dickson
Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
J Biol Chem 272:30196-200. 1997..Treatment of cells with dihydrosphingosine activates transcription of the TPS2 gene encoding a subunit of trehalose synthase and causes trehalose, a known thermoprotectant, to accumulate...
- Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiaeE Boy-Marcotte
Institut de Genetique et Microbiologie, Unité de Recherche Associée CNRS 2225, Universite Paris Sud, Orsay, France
J Bacteriol 180:1044-52. 1998..Thirty-nine gene products (including those of ALD3, GDH3, GLK1, GPP2, HSP104, HXK1, PGM2, SOD2, SSA3, SSA4, TKL2, TPS1, and YBR149W) are dependent upon Msn2/4p for their induction at the diauxic transition...
- Trehalose-6P synthase is essential for trehalase activation triggered by glucose, nitrogen source or heat shock, but not by osmostress, in Schizosaccharomyces pombeJ Cansado
Department of Genetics and Microbiology, University of Murcia, 30071 Murcia, Spain
Biochim Biophys Acta 1381:271-8. 1998Cells of Schizosaccharomyces pombe disrupted in the tps1+ gene, which encodes trehalose-6P synthase, were unable to increase trehalase activity in response to the addition of glucose or nitrogen source...
- Stimulation of proteinase activated receptor-2 causes endothelial cells to promote blood coagulation in vitroA K Alm
Department of Physiology and Neuroscience, Lund University, Sweden
Thromb Haemost 81:984-8. 1999..this growing receptor subclass, can be activated by trypsin and trypsin-like serine proteases such as mast cell tryptase. PAR-2 is expressed in endothelial cells...
- Delayed onset of inflammation in protease-activated receptor-2-deficient miceJ R Lindner
Cardiovascular Division and Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Immunol 165:6504-10. 2000..We hypothesized that activation of endothelial PAR2 by mast cell tryptase or other proteases also contributes to inflammatory responses...
- Glycosylation and the activation of proteinase-activated receptor 2 (PAR(2)) by human mast cell tryptaseS J Compton
Diabetes and Endocrine Research Group, Department of Pharmacology and Therapeutics University of Calgary, Faculty of Medicine, Calgary, AB, Canada
Br J Pharmacol 134:705-18. 20011. Human mast cell tryptase appears to display considerable variation in activating proteinase-activated receptor 2 (PAR(2))...
- Functional effects of protease-activated receptor-2 stimulation on human airway smooth muscleL S Chambers
Department of Pharmacology, University of Sydney, New South Wales 2006, Australia
Am J Physiol Lung Cell Mol Physiol 281:L1369-78. 2001..PAR)-2 is present on the smooth muscle and epithelium of human airways and can be activated by mast cell tryptase, trypsin, or the PAR-2 activating peptide (AP)...
- Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombeTeresa Soto
Department of Genetics and Microbiology, Facultad de Biologia, University of Murcia, Spain
Eur J Biochem 269:3847-55. 2002..we show that the products of the principal genes involved in trehalose metabolism in Schizosaccharomyces pombe, tps1+ (coding for trehalose-6-P synthase, Tps1p), ntp1+ (encoding neutral trehalase, Ntp1p) and tpp1+ (that codes for ..
- Gts1p stabilizes oscillations in energy metabolism by activating the transcription of TPS1 encoding trehalose-6-phosphate synthase 1 in the yeast Saccharomyces cerevisiaeZhaojun Xu
Department of Biochemistry 2, University of Yamanashi, Faculty of Medicine, 1110 Shimokato, Tamaho, Yamanashi 409 3898, Japan
Biochem J 383:171-8. 2004..Further, the expression of TPS1, encoding trehalose-6-phosphate synthase 1, and HSP104 was activated by Gts1p in combination with Snf1 kinase, a ..
- Tryptase activates phosphatidylinositol 3-kinases proteolytically independently from proteinase-activated receptor-2 in cultured dog airway smooth muscle cellsJames K Brown
Pulmonary and Critical Care Medicine Section, Dept of Veterans Affairs Medical Center, and Department of Medicine, University of California San Francisco, CA 94121, USA
Am J Physiol Lung Cell Mol Physiol 290:L259-69. 2006Mast cell tryptase is a potent mitogen for many cells in the airways and lung, but the cellular mechanisms for its growth stimulatory effects are poorly understood...
- Degranulating mast cells in fibrotic regions of human tumors and evidence that mast cell heparin interferes with the growth of tumor cells through a mechanism involving fibroblastsMichael Samoszuk
Department of Pathology and Laboratory Medicine, University of California, Irvine, California, USA
BMC Cancer 5:121. 2005..The purpose of this study was to test the hypothesis that mast cells that are present in fibrotic regions of cancer can suppress the growth of tumor cells through an indirect mechanism involving peri-tumoral fibroblasts...
- WRKY75 transcription factor is a modulator of phosphate acquisition and root development in ArabidopsisBallachanda N Devaiah
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907 1165, USA
Plant Physiol 143:1789-801. 2007..revealed that the expression of several genes involved in Pi starvation responses, including phosphatases, Mt4/TPS1-like genes, and high-affinity Pi transporters, was decreased when WRKY75 was suppressed...
- Adaptive evolution of metabolic pathways in DrosophilaJ M Flowers
Department of Ecology and Evolution, Stony Brook University, NY, USA
Mol Biol Evol 24:1347-54. 2007..evolution (glucose-6-phosphate dehydrogenase [G6pd]), phosphoglucomutase [Pgm], trehalose-6-phosphate synthetase [Tps1], and glucose-6phosphatase [G6pase] encode G6P branch point enzymes that catalyze reactions at the entry point to ..
- Trehalose is an important mediator of Cap1p oxidative stress response in Candida albicansYingying Cao
School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China
Biol Pharm Bull 31:421-5. 2008..Real time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcript levels of TPS2 and TPS3 were increased in the wild type strain compared to that in cap1/cap1 mutant when exposed to H2O2...
- Spontaneous cutaneous mast cell tumor with lymph node metastasis in a Richardson's ground squirrel (Spermophilus richardsonii)Xi Jun He
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
J Vet Diagn Invest 21:156-9. 2009..The results of immunohistochemistry indicated positive labeling for vimentin, mast cell tryptase, c-kit, and Ki-67. Toluidine blue stain revealed fine, metachromatic, cytoplasmic granules...
- Investigation of the inflammatory mechanisms in alopecia areataEmel Dikicioglu Cetin
Department of Pathology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey
Am J Dermatopathol 31:53-60. 2009..immunohistochemically with antibodies for CD1a, CD3, CD4, CD8, CD20, CD57 (for natural killer cells), mast cell tryptase, nerve growth factor receptor (NGFR), and substance P (SP). Various clinical findings were recorded...
- Correlation of plasma complement split product levels with allergic respiratory disease activity and relation to allergen immunotherapyCorinna Bowser
Department of Medicine, State University of New York SUNY Downstate Medical Center, Brooklyn, New York 11203, USA
Ann Allergy Asthma Immunol 104:42-9. 2010Allergens, including dust mite and grass pollen, and mast cell tryptase are known to generate the complement split products (CSPs) C5a and C3a, which can then trigger allergic inflammation...
- [Cloning and functional analysis of trehalose -6-phosphate synthase gene from Selaginella pulvinata]Jing Lin
Maize Research Institute, Sichuan Agricultural University, Ya an 625014, China
Yi Chuan 32:498-504. 2010..Yeast functional complementation test showed that trehalose-6-phosphate synthase mutant (tps1 big up tri, open), transformed by the ORF of SpTPS1 gene, can restore growth on the medium supplemented with ..
- Chronological assessment of mast cell-mediated gut dysfunction and mucosal inflammation in a rat model of chronic psychosocial stressMaria Vicario
Digestive Diseases Research Unit, Lab Neuro Immuno Gastroenterology, Institut de Recerca Vall d Hebron, CIBERehd, Department of Gastroenterology, Barcelona, Spain
Brain Behav Immun 24:1166-75. 2010..The notable resemblance of these findings with those in some IBS patients, support the potential interest and translational validity of this experimental model for the research of stress-sensitive intestinal disorders, particularly IBS...
- Mast cell tryptase stimulates production of decorin by human testicular peritubular cells: possible role of decorin in male infertility by interfering with growth factor signalingM Adam
Anatomy and Cell Biology, Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, D 80802 Munich, Germany
Hum Reprod 26:2613-25. 2011..In men with impaired spermatogenesis, fibrotic remodeling of these walls and accumulation of tryptase-positive mast cells (MCs) occur.
- Honeybee venom immunotherapy: a comparative study using purified and nonpurified aqueous extracts in patients with normal Basal serum tryptase concentrationsM Beatrice Bilò
Allergy Unit, Department of Allergy, Immunology and Respiratory Diseases, University Hospital Ospedali Riuniti, Via Conca 1, 60020 Ancona, Italy
J Allergy (Cairo) 2012:869243. 2012..preparation under the same build-up protocol in bee venom allergic patients with a normal baseline mast cell tryptase concentration...
- Usefulness and limitations of sequential serum tryptase for the diagnosis of anaphylaxis in 102 patientsAnna Sala-Cunill
Allergy Section, Internal Medicine Department, Hospital Universitari Vall d Hebron, Universitat Autonoma de Barcelona, Barcelona, Spain
Int Arch Allergy Immunol 160:192-9. 2013..The diagnosis of anaphylaxis is based on clinical history since no reliable biological marker is currently available to confirm the diagnosis...
- Mast cell tryptase levels in gut mucosa in patients with gastrointestinal symptoms caused by food allergyA F Hagel
Department of Medicine I, Functional Tissue Diagnostics, University Erlangen Nuremberg, Erlangen, Germany
Int Arch Allergy Immunol 160:350-5. 2013..The objective of this study was to investigate whether mast cell tryptase (T), a typical mast cell-associated marker, may help to detect patients with food allergy.
- Critical role of protease-activated receptor 2 activation by mast cell tryptase in the development of postoperative painSara M Oliveira
Department of Chemistry, Graduate Program in Biological Sciences Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil
Anesthesiology 118:679-90. 2013..Thus, the aim of this study was to investigate the involvement of mast cell degranulation, tryptase and its substrate, the protease-activated receptor 2, in a model of postoperative pain in mice.
- Praveen Thumbikat; Fiscal Year: 2016..We will perform rigorous clinical studies to validate the use of MCP-1 and MIP-11 and mast cell tryptase as biomarkers for CPPS in humans and correlate their levels with CPPS symptoms...
- Matthew J Hamilton; Fiscal Year: 2016..The human ortholog to mMCP-6 is Tryptase-[unreadable]1 which is the dominant mast cell tryptase in humans...
- RECOMBINANT HUMAN MAST CELL TRYPTASESDavid Johnson; Fiscal Year: 2000..In preliminary studies the principal investigator has expressed and purified fully active recombinant human skin tryptase 1 (rHST1) in Pichia pastoris...
- Kalpna Gupta; Fiscal Year: 2016..In turn, mast cell tryptase activates protease activated receptor 2 (PAR 2) on sensory nerve endings maintaining nociceptor sensitization and ..
- PLA2 activation by mast cell tryptase in ICJane McHowat; Fiscal Year: 2007..We hypothesize that these two processes participate in a vicious cycle of inflammation, with tryptase released from activated mast cells activating the protease-activated receptor-2 (PAR-2) on urothelial and ..
- MOLECULAR PATHOGENESIS OF RADIATION ENTEROPATHYMartin Hauer Jensen; Fiscal Year: 2005..appear to be mutually dependent; that protease-activated receptor 2 (PAR-2), which is activated by mast cell tryptase and mediates mitogenic and fibrogenic responses, is upregulated in intestinal radiation fibrosis; and that ..
- PAR2, Bronchopulmonary Sensory Nerve and Pathogenesis of AsthmaQihai Gu; Fiscal Year: 2009..Our hypothesis is that when PAR2 is activated by endogenous (e.g., mast cell tryptase and trypsin-like proteases) or exogenous (e.g...
- BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMESLawrence Schwartz; Fiscal Year: 2000..The major component of the secretory granules of all human mast cells on a weight basis is the tryptase family of serine proteases...
- MECHANISMS AND MODULATION OF VISCERAL PAINGerald Gebhart; Fiscal Year: 2009..We also hypothesize that protons and/or endogenous mediators (e.g., ATP, mast cell tryptase, serotonin) contribute to visceral hypersensitivity and can do so in the absence of frank tissue damage, which is ..
- EFFECTS OF O3 AND NO2 ON HUMAN LUNG PROTEINSDavid Johnson; Fiscal Year: 1992..b>Tryptase is the principal granular protein of human mast cells...
- THE ROLE OF CTGF IN SUBEPITHELIAL FIBROSIS IN ASTHMADAVID RISHIKOF; Fiscal Year: 2004..Preliminary data indicate that the cell products TGF-beta and IL-4, and trypsin (a protease related to mast cell tryptase) induce CTGF and type I collagen in fibroblasts...
- BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMESLawrence Schwartz; Fiscal Year: 2005..The major component of the secretory granules of all human mast cells on a weight basis is the tryptase family of serine proteases...
- Flourescent Ceramic Nanoprobes (RMI)TIMOTHY BOYLE; Fiscal Year: 2008..All of the innovations uncovered during the preparation of these nanoprobes will be shared in order to benefit medical research applications beyond those proposed in this investigation. ..
- CHARACTERIZATION OF DIFFERENT TYPES OF HUMAN MAST CELLSLawrence Schwartz; Fiscal Year: 2009..Aim 1 examines new functional and phenotypic characteristics that distinguish MCT (tryptase+/chymase-) from MCTC (tryptase+/chymase-)types of human mast cells...
- MECHANISM OF HUMAN MAST CELL TRYPTASE REGULATIONNorman Schechter; Fiscal Year: 2003..of this work is to describe the biochemical mechanism for the functional instability of the serine protease human tryptase and to define the relevance of this mechanism to the regulation of tryptase in vivo...
- SERINE PROTEASES FROM MAST CELLS AND SKINNorman Schechter; Fiscal Year: 2002..It is hoped that these studies will advance our understanding of two classes of molecules which are important to production and regulation of inflammation. ..
- EVALUATION OF BASOPHIL INVOLVEMENT IN HUMAN DISEASELawrence Schwartz; Fiscal Year: 2001..In humans, mast cell involvement can be precisely identified by the presence of tryptase. For basophils, no such direct marker has been identified...