tryptase

Summary

Gene Symbol: tryptase
Description: tryptase alpha/beta 1
Alias: TPS1, TPS2, TPSB1, tryptase alpha/beta-1, mast cell alpha II tryptase, mast cell beta I tryptase, tryptase alpha II, tryptase alpha-1, tryptase beta I, tryptase beta-1, tryptase-1, tryptase-I, tryptase-III
Species: human

Top Publications

  1. ncbi Mast cell tryptase and hemolysis after trauma
    Erik Edston
    Department of Forensic Medicine, University Hospital, S 581 85 Linkoping, Sweden
    Forensic Sci Int 131:8-13. 2003
  2. ncbi Tryptase activates peripheral blood mononuclear cells causing the synthesis and release of TNF-alpha, IL-6 and IL-1 beta: possible relevance to multiple sclerosis
    Vivian Malamud
    Department of Cellular Biochemistry and Human Genetics, Hebrew University, Hadassah Medical School, P O Box 12272, 91120 Jerusalem, Israel
    J Neuroimmunol 138:115-22. 2003
  3. pmc Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease family
    P Vanderslice
    Cardiovascular Research Institute, University of California, San Francisco 94143
    Proc Natl Acad Sci U S A 87:3811-5. 1990
  4. pmc Cloning and characterization of a second complementary DNA for human tryptase
    J S Miller
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298
    J Clin Invest 86:864-70. 1990
  5. pmc Cloning and characterization of complementary DNA for human tryptase
    J S Miller
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298
    J Clin Invest 84:1188-95. 1989
  6. ncbi The fibrinogenolytic activity of purified tryptase from human lung mast cells
    L B Schwartz
    J Immunol 135:2762-7. 1985
  7. ncbi Characterization of a tryptase mRNA expressed in the human basophil cell line KU812
    T Blom
    Department of Immunology, Biomedicum, University of Uppsala, Sweden
    Scand J Immunol 37:203-8. 1993
  8. ncbi Mast cell tryptase is a mitogen for epithelial cells. Stimulation of IL-8 production and intercellular adhesion molecule-1 expression
    J A Cairns
    Immunopharmacology Group, Southampton General Hospital, UK
    J Immunol 156:275-83. 1996
  9. pmc The alpha form of human tryptase is the predominant type present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosis
    L B Schwartz
    Department of Medicine, Virginia Commonwealth University, Richmond 23298, USA
    J Clin Invest 96:2702-10. 1995
  10. pmc Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblasts
    J A Cairns
    Immunopharmacology Group, Southampton General Hospital, United Kingdom
    J Clin Invest 99:1313-21. 1997

Research Grants

  1. Praveen Thumbikat; Fiscal Year: 2016
  2. Matthew J Hamilton; Fiscal Year: 2016
  3. RECOMBINANT HUMAN MAST CELL TRYPTASES
    David Johnson; Fiscal Year: 2000
  4. Kalpna Gupta; Fiscal Year: 2016
  5. PLA2 activation by mast cell tryptase in IC
    Jane McHowat; Fiscal Year: 2007
  6. MOLECULAR PATHOGENESIS OF RADIATION ENTEROPATHY
    Martin Hauer Jensen; Fiscal Year: 2005
  7. PAR2, Bronchopulmonary Sensory Nerve and Pathogenesis of Asthma
    Qihai Gu; Fiscal Year: 2009
  8. BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMES
    Lawrence Schwartz; Fiscal Year: 2000
  9. MECHANISMS AND MODULATION OF VISCERAL PAIN
    Gerald Gebhart; Fiscal Year: 2009
  10. EFFECTS OF O3 AND NO2 ON HUMAN LUNG PROTEINS
    David Johnson; Fiscal Year: 1992

Detail Information

Publications203 found, 100 shown here

  1. ncbi Mast cell tryptase and hemolysis after trauma
    Erik Edston
    Department of Forensic Medicine, University Hospital, S 581 85 Linkoping, Sweden
    Forensic Sci Int 131:8-13. 2003
    We have previously found increased mast cell tryptase in accidental deaths due to trauma, indicating that mast cell degranulation had occurred...
  2. ncbi Tryptase activates peripheral blood mononuclear cells causing the synthesis and release of TNF-alpha, IL-6 and IL-1 beta: possible relevance to multiple sclerosis
    Vivian Malamud
    Department of Cellular Biochemistry and Human Genetics, Hebrew University, Hadassah Medical School, P O Box 12272, 91120 Jerusalem, Israel
    J Neuroimmunol 138:115-22. 2003
    ..The most abundant secretory mediator of the human mast cell is the tetrameric protease tryptase. We demonstrate that tryptase can activate peripheral mononuclear cells (PBMCs), isolated from healthy donors as ..
  3. pmc Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease family
    P Vanderslice
    Cardiovascular Research Institute, University of California, San Francisco 94143
    Proc Natl Acad Sci U S A 87:3811-5. 1990
    Three different cDNAs and a gene encoding human skin mast cell tryptase have been cloned and sequenced in their entirety. The deduced amino acid sequences reveal a 30-amino acid prepropeptide followed by a 245-amino acid catalytic domain...
  4. pmc Cloning and characterization of a second complementary DNA for human tryptase
    J S Miller
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298
    J Clin Invest 86:864-70. 1990
    A second cDNA for human tryptase, called beta-tryptase, was cloned from a mast cell cDNA library in lambda ZAP...
  5. pmc Cloning and characterization of complementary DNA for human tryptase
    J S Miller
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298
    J Clin Invest 84:1188-95. 1989
    The amino acid sequence of human mast cell tryptase was determined from corresponding cDNA cloned from a lambda ZAP library made with mRNA derived from a human mast cell preparation...
  6. ncbi The fibrinogenolytic activity of purified tryptase from human lung mast cells
    L B Schwartz
    J Immunol 135:2762-7. 1985
    The capacity of purified tryptase from human lung mast cells to metabolize human fibrinogen, fibrin, and plasminogen was evaluated...
  7. ncbi Characterization of a tryptase mRNA expressed in the human basophil cell line KU812
    T Blom
    Department of Immunology, Biomedicum, University of Uppsala, Sweden
    Scand J Immunol 37:203-8. 1993
    ..PCR primers designed for the amplification of a nearly full-length copy of tryptase mRNAs were used to study the identity of the KU812 tryptase...
  8. ncbi Mast cell tryptase is a mitogen for epithelial cells. Stimulation of IL-8 production and intercellular adhesion molecule-1 expression
    J A Cairns
    Immunopharmacology Group, Southampton General Hospital, UK
    J Immunol 156:275-83. 1996
    b>Tryptase, a protease unique to the mast cell secretory granule, is released in substantial quantities into the respiratory tract of patients with inflammatory disease of the airways...
  9. pmc The alpha form of human tryptase is the predominant type present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosis
    L B Schwartz
    Department of Medicine, Virginia Commonwealth University, Richmond 23298, USA
    J Clin Invest 96:2702-10. 1995
    b>Tryptase, a protease produced by all mast cells, was evaluated as a clinical marker of systemic mastocytosis. Two sandwich immunoassays were evaluated, one which used the mAb G5 for capture, the other which used B12 for capture...
  10. pmc Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblasts
    J A Cairns
    Immunopharmacology Group, Southampton General Hospital, United Kingdom
    J Clin Invest 99:1313-21. 1997
    ..We have investigated the potential of tryptase, the major protease of human mast cells, to stimulate collagen synthesis in the human lung fibroblast cell line ..
  11. ncbi Human mast cell tryptase fibrinogenolysis: kinetics, anticoagulation mechanism, and cell adhesion disruption
    V A Thomas
    Department of Biochemistry and Molecular Biology, J H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0581, USA
    Biochemistry 37:2291-8. 1998
    b>Tryptase is a 31 kDa, glycosylated, trypsin-like enzyme stored in and released from mast cell granules...
  12. ncbi Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3
    M Pallaoro
    Cardiovascular Research Institute and Departments of Medicine and Laboratory Medicine, University of California, San Francisco, California 94143 0911, USA
    J Biol Chem 274:3355-62. 1999
    ..They fall into two groups, alpha and beta. Although several related tryptase mRNAs are known, it is unclear which if any are transcripts of separate haploid genes...
  13. ncbi Human tryptases alpha and beta/II are functionally distinct due, in part, to a single amino acid difference in one of the surface loops that forms the substrate-binding cleft
    C Huang
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 274:19670-6. 1999
    ..Structural modeling analyses revealed that tryptase alpha differs from tryptase beta/II in that it possesses an Asp, rather than a Gly, in one of the loops that form ..
  14. ncbi Characterization of human gamma-tryptases, novel members of the chromosome 16p mast cell tryptase and prostasin gene families
    G H Caughey
    Cardiovascular Research Institute and Department of Medicine, University of California, San Francisco 94143, USA
    J Immunol 164:6566-75. 2000
    Previously, this laboratory identified clusters of alpha-, beta-, and mast cell protease-7-like tryptase genes on human chromosome 16p13.3...
  15. ncbi Characterization of two highly polymorphic human tryptase loci and comparison with a newly discovered monkey tryptase ortholog
    M Guida
    PPGx Incorporated, La Jolla, CA 92037, USA
    Pharmacogenetics 10:389-96. 2000
    ..data on the tryptase inhibitors undergoing clinical trials, we defined the genetic variability of the tryptase 1 (TPS1) and tryptase 2 (TPS2) loci by screening a reference population of 32 individuals representing three major ..
  16. ncbi Genetic deficiency of human mast cell alpha-tryptase
    D Soto
    Cardiovascular Research Institute and Department of Medicine, University of California at San Francisco, San Francisco, California 94143 0911, USA
    Clin Exp Allergy 32:1000-6. 2002
    ..Human alpha- and beta-tryptases are proteases secreted by mast cells. Beta (but not alpha) tryptases are implicated in asthma. Genes encoding both types of tryptases cluster on chromosome 16p13.3...
  17. pmc Tryptase haplotype in mastocytosis: relationship to disease variant and diagnostic utility of total tryptase levels
    Cem Akin
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA
    Clin Immunol 123:268-71. 2007
    Serum mast cell tryptase levels are used as a diagnostic criterion and surrogate marker of disease severity in mastocytosis...
  18. pmc Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation
    Nicole E Jackson
    Centre for Infection and Inflammation Research, School of Medical Sciences, Sydney, New South Wales 2052, Australia
    J Biol Chem 283:34178-87. 2008
    ..This model explains why many of the preferred substrates of tryptase are short peptides; however, it does not explain how tryptase cleaves large protein substrates such as ..
  19. pmc Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations
    Neil N Trivedi
    Cardiovascular Research Institute, University of California at San Francisco, San Francisco, Calif, USA
    J Allergy Clin Immunol 124:1099-105.e1-4. 2009
    Mast cell tryptases have proposed roles in allergic inflammation and host defense against infection. Tryptase gene loci TPSAB1 and TPSB2 are known to be polymorphic, but the nature and extent of diversity at these loci have not been fully ..
  20. doi Serum total tryptase levels are increased in patients with active chronic urticaria
    M Ferrer
    Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra Medical School, Pamplona, Spain
    Clin Exp Allergy 40:1760-6. 2010
    ..We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation...
  21. pmc Mast cell tryptase deficiency attenuates mouse abdominal aortic aneurysm formation
    Jie Zhang
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Circ Res 108:1316-27. 2011
    ..b>Tryptase is the most abundant MC granule protein and participates in MC activation, protease maturation, leukocyte ..
  22. doi Tissue remodelling in breast cancer: human mast cell tryptase as an initiator of myofibroblast differentiation
    Anita Mangia
    Clinical Experimental Oncology Laboratory, National Cancer Centre Giovanni Paolo II, University of Bari, Bari, Italy
    Histopathology 58:1096-106. 2011
    ..We investigated α-smooth-muscle actin (α-SMA(+) ) and CD34(+) fibroblasts, density of toluidine blue-stained (MCs-TB) and tryptase-immunolabelled MCs (MCs-Try) in 30 primary breast tumours.
  23. pmc Immunoglobulin E and mast cell proteases are potential risk factors of human pre-diabetes and diabetes mellitus
    Zhen Wang
    Department of Clinical Medicine, School of Medicine, Huzhou Teachers College, Huzhou, Zhejiang, China
    PLoS ONE 6:e28962. 2011
    ..Plasma levels of mast cell proteases and the mast cell activator immunoglobulin E (IgE) may serve as novel inflammatory markers that associate with the risk of pre-diabetes and diabetes mellitus...
  24. ncbi Immunoreactivity for CD25 in gastrointestinal mucosal mast cells is specific for systemic mastocytosis
    Hejin P Hahn
    Department of Pathology, Brigham and Women s Hospital, and Harvard Medical School, Boston, MA 02115, USA
    Am J Surg Pathol 31:1669-76. 2007
    ..infections, and 9 biopsies from 7 patients with eosinophilic gastroenteritis were immunostained for mast cell tryptase, c-kit (CD117), and CD25...
  25. ncbi Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells
    Xue Qiong Zhu
    Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
    Gynecol Oncol 105:650-6. 2007
    ..The purpose of this study was to reveal the different mast cell subsets in smooth muscle tumors of uterus and to investigate the mechanism of local increase of mast cells...
  26. ncbi Mastocytic enterocolitis: increased mucosal mast cells in chronic intractable diarrhea
    Shriram Jakate
    Department of Pathology, Rush University Medical Center, Chicago, IL 60612, USA
    Arch Pathol Lab Med 130:362-7. 2006
    ..In some adult patients with chronic intractable diarrhea, the diagnosis remains elusive even after detailed evaluations, and colonic or duodenal biopsy specimens may appear unremarkable on routine hematoxylin-eosin staining...
  27. ncbi Mast cells cultured from the peripheral blood of normal donors and patients with mastocytosis originate from a CD34+/Fc epsilon RI- cell population
    M Rottem
    Allergic Diseases Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
    Blood 84:2489-96. 1994
    ..cell number and cell differential using Wright Giemsa and acid toluidine blue stains and antibodies to mast cell tryptase and chymase, cell-associated histamine, and expression of CD34, c-kit, Fc epsilon RI, and Fc gamma RII using flow ..
  28. ncbi Eosinophilic gastroenterocolitis in iron lactate-overloaded rats
    I Narama
    Research Institute of Drug Safety, Setsunan University, Osaka, Japan
    Toxicol Pathol 27:318-24. 1999
    ..The morphologic characteristics of the gastrointestinal lesions and peripheral eosinophilia induced in rats fed iron lactate were very similar to those in some cases of eosinophilic gastroenterocolitis in humans and other animals...
  29. pmc Increased mast cells and neutrophils in submucosal mucous glands and mucus plugging in patients with asthma
    N G Carroll
    Faculty of Regional Professional Studies, Edith Cowan University, Bunbury 6230, Australia
    Thorax 57:677-82. 2002
    ..A study was undertaken to count the mast cells and neutrophils in submucosal glands and to relate cell numbers to the presence of mucus in the airway lumen...
  30. ncbi Proteinase-activated receptor (PAR)-1 and -2 agonists induce mediator release from mast cells by pathways distinct from PAR-1 and PAR-2
    Grant R Stenton
    Pulmonary Research Group, Department of Medicine, Astra Zeneca Canada Inc, Glaxo Heritage Asthma Research Laboratory, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Pharmacol Exp Ther 302:466-74. 2002
    ..from peritoneal mast cells, whereas another PAR-2AP (SLIGRL-NH(2), 10 microM), trypsin (40 U/ml), and mast cell tryptase (1.5 microg/ml) did not. PAR-1AP (ApfFRChaCitY-NH(2), 10 microM) (Cit) induced 11.7 +/- 3...
  31. ncbi Association of mast cells with calcification in the human pineal gland
    Danuta Maslinska
    Department of Experimental and Clinical Neuropathology, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego, Warsaw
    Folia Neuropathol 48:276-82. 2010
    ..stained with haematoxylin and eosin or the von Kossa staining technique and for the distribution of mast cell tryptase, mast cell chymase, histamine H4 receptor and vascular network using biotinylated Ulex europaeus agglutinin...
  32. doi Vasoactive intestinal peptide (VIP) treatment of Parkinsonian rats increases thalamic gamma-aminobutyric acid (GABA) levels and alters the release of nerve growth factor (NGF) by mast cells
    Orhan Tansel Korkmaz
    Department of Physiology, Medical Faculty, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey
    J Mol Neurosci 41:278-87. 2010
    ..Therefore, the protective effect of VIP on motor function is possibly related to the increased levels of GABA in the VATh, and its neuroprotective actions may be mediated by the release of NGF from brain mast cells...
  33. ncbi A sensitive colorimetric assay for the release of tryptase from human lung mast cells in vitro
    S E Lavens
    Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, UK
    J Immunol Methods 166:93-102. 1993
    ..We have used a simple colorimetric assay for tryptase to follow the release of proteolytic enzymes from human lung mast cells in vitro...
  34. ncbi Activated mast cells in proximity to colonic nerves correlate with abdominal pain in irritable bowel syndrome
    Giovanni Barbara
    Departmentof Internal Medicine and Gastroenterology, and CRBA, University of Bologna, Italy
    Gastroenterology 126:693-702. 2004
    ..We assessed colonic mast cell infiltration, mediator release, and spatial interactions with mucosal innervation and their correlation with abdominal pain in IBS patients...
  35. ncbi Expression of mast cell proteases in rat lung during helminth infection: mast cells express both rat mast cell protease II and tryptase in helminth infected lung
    M Tomita
    Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki, Japan
    Int Arch Allergy Immunol 120:303-9. 1999
    ..However, a previous study of ours showed that the expression of rat mast cell tryptase (RMCT) mRNA, which has been reported to be confined to CTMC, significantly increased in rat lung 14 days after ..
  36. pmc Mast cells: the forgotten cells of renal fibrosis
    I S Roberts
    Department of Cellular Pathology, Level 1, John Radcliffe Hospital, Headley Way, Headington, Oxford OX3 9DU, UK
    J Clin Pathol 53:858-62. 2000
    ..Therefore, the numbers of mast cells in normal and fibrotic kidneys with various pathologies were investigated...
  37. ncbi Contribution of mast cells to the tubulointerstitial lesions in IgA nephritis
    T Ehara
    First Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
    Kidney Int 54:1675-83. 1998
    ..Mast cells have been found to secrete basic fibroblast growth factor (bFGF) in human pulmonary fibrosis...
  38. ncbi Inhibition of keratinocyte growth in cell culture and whole skin culture by mast cell mediators
    M Huttunen
    Department of Dermatology, Kuopio University Hospital, Kuopio, Finland
    Exp Dermatol 10:184-92. 2001
    ..in a 24-well plate, and the cells were treated with different mast cell mediators histamine, heparin and tryptase, or lysate from HMC-1 cells, a human leukemic mast cell line...
  39. ncbi A pilot study of fecal serine-protease activity: a pathophysiologic factor in diarrhea-predominant irritable bowel syndrome
    Richard Roka
    Institut National de la Recherche Agronomique, Neuro Gastroenterology and Nutrition Unit, Toulouse, France
    Clin Gastroenterol Hepatol 5:550-5. 2007
    ..Our study was designed to evaluate if colonic luminal serine-proteases may be a relevant pathophysiologic marker of IBS...
  40. ncbi Protease-activated receptor 2 expression in trigeminal neurons innervating the rat nasal mucosa
    Q Thai Dinh
    Department of Internal Medicine, Charite School of Medicine, Humboldt University, Augustenburger Platz 1, 13353 Berlin, Germany
    Neuropeptides 39:461-6. 2005
    Protease-activated receptor 2 (PAR2) is activated by trypsin and mast cell tryptase to induce widespread inflammation by unknown mechanisms...
  41. ncbi Human mast cells express functional TrkA and are a source of nerve growth factor
    G Nilsson
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA
    Eur J Immunol 27:2295-301. 1997
    ..Using flow cytometric analysis and mast cell tryptase as a mast cell marker, both HMC-1 cells and cultured human mast cells were shown to coexpress tryptase and TrkA...
  42. ncbi Cathepsin B activates human trypsinogen 1 but not proelastase 2 or procarboxypeptidase B
    Bjorn Lindkvist
    Department of Surgery, Malmo University Hospital, Lund University, Malmo, Sweden
    Pancreatology 6:224-31. 2006
    ..not known although suggested explanations include autoactivation, cathepsin B-mediated activation and activation by mast cell tryptase. The aim of this study was to investigate cathepsin B and tryptase activation of pancreatic zymogens.
  43. ncbi Mast cell tryptase causes homologous desensitization of beta-adrenoceptors by Ca2+ sensitization in tracheal smooth muscle
    M Kobayashi
    Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Clin Exp Allergy 38:135-44. 2008
    Recent studies have revealed that in asthma, mast cells infiltrate to the smooth muscle layer and release tryptase, an enzymatic activator of protease-activated receptor 2 (PAR2)...
  44. doi Lipid-rich enteral nutrition reduces postoperative ileus in rats via activation of cholecystokinin-receptors
    Tim Lubbers
    Department of Surgery, Nutrition and Toxicology Research Institute Maastricht, Maastricht University Medical Centre, Maastricht, The Netherlands
    Ann Surg 249:481-7. 2009
    ..This study investigates the effect of lipid-rich nutrition on the local inflammatory response and gastrointestinal hypomotility in a rat model of postoperative ileus...
  45. ncbi Protease-activated receptor-2 mediates proliferative responses in skeletal myoblasts
    C Chinni
    School of Veterinary Science, University of Melbourne, Parkville, Victoria 3010, Australia
    J Cell Sci 113:4427-33. 2000
    ..Activators of PAR-2 include trypsin and mast cell tryptase. Skeletal myoblasts are known to express PAR-1, a thrombin receptor...
  46. pmc Localized Th1-, Th2-, T regulatory cell-, and inflammation-associated hepatic and pulmonary immune responses in Ascaris suum-infected swine are increased by retinoic acid
    Harry Dawson
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, U S Department of Agriculture, Beltsville, MD 20705, USA
    Infect Immun 77:2576-87. 2009
    ..T regulatory (Treg) cells, while infected pigs given ATRA had higher IL4, IL13, CCL11, CCL26, CCL17, CCL22, and TPSB1 expression...
  47. doi Expulsion of secondary Trichinella spiralis infection in rats occurs independently of mucosal mast cell release of mast cell protease II
    Lisa K Blum
    James A Baker Institute for Animal Health, Cornell University, Ithaca, NY 14853, USA
    J Immunol 183:5816-22. 2009
    ..Furthermore, release of RMCPII is not sufficient to cause expulsion. The data argue against a role for mast cells in the mechanism underlying the effector phase of protective immunity against T. spiralis in rats...
  48. ncbi Mast cell tryptase in postmortem serum-reference values and confounders
    Erik Edston
    Institution for Clinical and Molecular Medicine, Linkoping University, Linköping 581 85, Sweden
    Int J Legal Med 121:275-80. 2007
    We have investigated the effects of some factors suspected of inducing spuriously increased tryptase concentrations, specifically sampling site, conjunctival petechial bleeding and prone position at the time of death as indicators of ..
  49. doi Local production of total IgE and specific antibodies to the house dust mite in adenoid tissue
    Seung Youp Shin
    Department of Otorhinolaryngology, College of Medicine, Kyunghee University, Seoul, Korea
    Pediatr Allergy Immunol 20:134-41. 2009
    ..homogenate were performed to quantify the levels of total IgE, eosinophil cationic protein (ECP), and mast cell tryptase. DP-specific IgE, IgA, IgG1, and IgG4 antibodies, soluble IL-2 receptors (sIL-2R), soluble CD23 (sCD23), and IL-6 ..
  50. pmc A comparative study of the spatial distribution of mast cells and microvessels in the foetal, adult human thymus and thymoma
    Marius Raica
    Department of Histology and Cytology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
    Int J Exp Pathol 91:17-23. 2010
    ..Double staining with CD34 and mast cell tryptase was used to count both mast cells and microvessels in the same fields...
  51. ncbi Genomic organization and chromosomal localization of the human cathepsin G gene
    P A Hohn
    Department of Medicine, Jewish Hospital, Washington University Medical Center, St Louis, Missouri 63110
    J Biol Chem 264:13412-9. 1989
    ..Having defined the genomic structure and chromosomal location of cathepsin G, we are now attempting to identify the DNA elements in or near this gene that mediate its tissue and development-specific pattern of expression...
  52. pmc The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-beta-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damage
    Christiane Schnee
    Max Planck Institute for Chemical Ecology, Winzerlaer Strasse 10, D 07745 Jena, Germany
    Plant Physiol 130:2049-60. 2002
    ..We isolated a terpene synthase gene, terpene synthase 1 (tps1), from maize that exhibits only a low degree of sequence identity to previously identified terpene synthases...
  53. doi Three trehalose synthetic pathways in the acarbose-producing Actinoplanes sp. SN223/29 and evidence for the TreY role in biosynthesis of component C
    Jin Sook Lee
    Institute of Molecular Microbiology and Biotechnology, Westfällische Wilhelms Universität Münster, Munster, Germany
    Appl Microbiol Biotechnol 80:767-78. 2008
    In this study, three trehalose gene clusters, treX-Y-Z, tpS1, and treS, of the acarbose-producing strain, Actinoplanes sp. SN223/29, have been identified...
  54. ncbi Delayed embryo development in the ARABIDOPSIS TREHALOSE-6-PHOSPHATE SYNTHASE 1 mutant is associated with altered cell wall structure, decreased cell division and starch accumulation
    Leonardo D Gomez
    CNAP, Department of Biology, University of York, Heslington, York YO10 5YW, UK
    Plant J 46:69-84. 2006
    The tps1 mutant, which is disrupted in the TREHALOSE-6-PHOSPHATE SYNTHASE 1 gene, has been previously characterized as a recessive embryo lethal. tps1 embryos do not develop past late torpedo or early cotyledon stage...
  55. ncbi Novel alleles of yeast hexokinase PII with distinct effects on catalytic activity and catabolite repression of SUC2
    S Hohmann
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Heverlee, Flanders, Belgium
    Microbiology 145:703-14. 1999
    ..Mutants lacking the TPS1 gene, which encodes trehalose 6-phosphate synthase, can not grow on glucose or fructose...
  56. ncbi Gene and protein expression of protease-activated receptor 2 in structural and inflammatory cells in the nasal mucosa in seasonal allergic rhinitis
    Q T Dinh
    Department of Internal Medicine, Charite Universitatsmedizin Berlin, Berlin, Germany
    Clin Exp Allergy 36:1039-48. 2006
    Protease-activated receptor 2 (PAR 2) has been shown to be responsible for trypsin and mast cell tryptase-induced airway inflammation...
  57. pmc The trehalose synthesis pathway is an integral part of the virulence composite for Cryptococcus gattii
    Popchai Ngamskulrungroj
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Infect Immun 77:4584-96. 2009
    ..Three genes putatively involved in trehalose biosynthesis, TPS1 (trehalose-6-phosphate [T6P] synthase) and TPS2 (T6P phosphatase), and degradation, NTH1 (neutral trehalose), were ..
  58. ncbi [The molecular composition and spectral properties of polysaccharide isolated from pu-erh tea and its material]
    Jia Shun Gong
    College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China
    Guang Pu Xue Yu Guang Pu Fen Xi 30:1960-4. 2010
    ..The main polysaccharide TPS2 and TPS1 were isolated and purified from pu-erh tea and its materials by DEAE-52 and Sephadex G-150 column ..
  59. doi Glycoconjugate expression on the cell wall of tps1/tps1 trehalose-deficient Candida albicans strain and implications for its interaction with macrophages
    María Martínez-Esparza
    Facultad de Medicina, Departamento de Bioquimica, Biología Molecular B e Inmunología, Universidad de Murcia, Murcia, Spain
    Glycobiology 21:796-805. 2011
    ..The gene-encoding trehalose-6-phosphate synthase (TPS1), which is responsible for trehalose synthesis, is induced in response to oxidative stress, as in phagolysosomes...
  60. ncbi Interactions of mast cell tryptase with thrombin receptors and PAR-2
    M Molino
    Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 272:4043-9. 1997
    b>Tryptase is a serine protease secreted by mast cells that is able to activate other cells...
  61. ncbi Eosinophils are activated in middle ear mucosa and middle ear effusion of patients with intractable otitis media associated with bronchial asthma
    Y Iino
    Department of Otolaryngology, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi ku, Tokyo 173 8605, Japan
    Clin Exp Allergy 31:1135-43. 2001
    ....
  62. doi Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitis
    Akira Matsuda
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Invest Ophthalmol Vis Sci 50:2871-7. 2009
    ....
  63. ncbi The orlA gene from Aspergillus nidulans encodes a trehalose-6-phosphate phosphatase necessary for normal growth and chitin synthesis at elevated temperatures
    P T Borgia
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 1120, USA
    Mol Microbiol 20:1287-96. 1996
    ..Da) that is homologous to a 211-amino-acid domain from the polypeptide encoded by the Saccharomyces cerevisiae TPS2 gene and to almost the entire Escherichia coli otsB-encoded polypeptide...
  64. ncbi Dietary wheat germ agglutinin modulates ovalbumin-induced immune responses in Brown Norway rats
    B Watzl
    Institute of Nutritional Physiology, Institute of Hygiene and Toxicology, Federal Research Centre for Nutrition, Haid und Neu Str, 9, 76131 Karlsruhe, Germany
    Br J Nutr 85:483-90. 2001
    ..In conclusion, these data suggest that high dietary intake of lectins such as WGA may affect the allergic response towards oral antigens in the gut-associated lymphoid tissue...
  65. ncbi Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulans
    S Fillinger
    Unité Microbiologie et Environnement, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbiology 147:1851-62. 2001
    ..Expression of tpsA in a Saccharomyces cerevisiae tps1 mutant revealed that the tpsA gene product is a functional equivalent of the yeast Tps1 trehalose-6-phosphate ..
  66. ncbi Basic fibroblast growth factor and its receptors in idiopathic pulmonary fibrosis and lymphangioleiomyomatosis
    Yoshikazu Inoue
    Division of Environmental and Occupational Health Sciences and Pulmonary Division, Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado, USA
    Am J Respir Crit Care Med 166:765-73. 2002
    ..We performed quantitative immunohistochemistry for bFGF, mast cell tryptase, smooth muscle actin for smooth muscle cell/myofibroblast-like cells, and fibroblast growth factor receptors (Flg,..
  67. doi Systemic mastocytosis involving the gastrointestinal tract: clinicopathologic and molecular study of five cases
    Richard Kirsch
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Toronto, ON, Canada
    Mod Pathol 21:1508-16. 2008
    ..Histologic features were documented in slides stained with hematoxylin and eosin, mast cell tryptase and CD117...
  68. pmc Truncation of Arabidopsis thaliana and Selaginella lepidophylla trehalose-6-phosphate synthase unlocks high catalytic activity and supports high trehalose levels on expression in yeast
    Patrick Van Dijck
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven and Vlaams Interuniversitair Instituut voor Biotechnologie VIB, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Flanders, Belgium
    Biochem J 366:63-71. 2002
    ..lepidophylla, being a notable exception. Overexpression of the plant genes in a Saccharomyces cerevisiae tps1 mutant results in very low TPS-catalytic activity and trehalose accumulation...
  69. ncbi Stem cell factor in a rat model of serum nephrotoxic nephritis
    M M El Kossi
    Sheffield Kidney Institute, University of Sheffield, Sheffield, UK
    Nephron Exp Nephrol 108:e1-e10. 2008
    ..The growth factor (SCF) was evaluated in a rat model of nephrotoxic serum nephritis (NTN), characterized by early inflammation followed by later tissue fibrosis...
  70. doi Mast cell burden and reticulin fibrosis in the myeloproliferative neoplasms: a computer-assisted image analysis study
    Arsalan Ahmed
    The Department of Pathology and Laboratory Medicine, The Methodist Hospital and Research Institute, Houston, TX 77030, USA
    Pathol Res Pract 205:634-8. 2009
    ..We used computer-assisted image analysis of bone marrow core biopsies stained for mast cell tryptase from patients with myeloproliferative neoplasms [31 cases: 12 chronic myelogenous leukemia (CML), 6 primary ..
  71. doi Evidence for the involvement of free light chain immunoglobulins in allergic and nonallergic rhinitis
    Desmond G Powe
    Department of Histopathology, Surgical and Medical Sciences, Queen s Medical Centre, Nottingham University Hospitals Trust, Nottingham NG7 2UH, United Kingdom
    J Allergy Clin Immunol 125:139-45.e1-3. 2010
    ..Recent murine studies suggest the existence of an IgE-independent hypersensitivity response involving antigen-induced mast cell activation, mediated by immunoglobulin free light chains (FLCs)...
  72. doi Lymphocyte sub-populations and non-Langerhans' cell monocytoid cells in pulmonary Langerhans' cell histiocytosis
    R Nagarjun Rao
    Department of Pathology, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    Pathol Res Pract 204:315-22. 2008
    ..for lymphohistiocytic markers: CD1a, CD3, CD4, CD8, CD15, CD20, CD56, TIA-1, CD68-PGM1, Mac387, and mast cell tryptase. Double immunolabeling was performed with CD1a/Mac387...
  73. ncbi Prophylaxis with ketotifen in rats with portal hypertension: involvement of mast cell and eicosanoids
    Fernando Sanchez-Patan
    Department of Surgery I, School of Medicine, Complutense University of Madrid, Spain
    Hepatobiliary Pancreat Dis Int 7:383-94. 2008
    ....
  74. ncbi Mast cell tryptase from pig lungs triggers infection by pneumotropic Sendai and influenza A viruses. Purification and characterization
    Y Chen
    Division of Enzyme Chemistry, Institute for Enzyme Research, University of Tokushima, Japan
    Eur J Biochem 267:3189-97. 2000
    ..Heparin stabilized the enzyme, but high-ionic-strength conditions did not, unlike for human mast cell tryptase. The purified enzyme efficiently processed the fusion glycoprotein precursor of Sendai virus and slowly processed ..
  75. pmc Serum tryptase monitoring in indolent systemic mastocytosis: association with disease features and patient outcome
    Almudena Matito
    Instituto de Estudios de Mastocitosis de Castilla La Mancha, Hospital Virgen del Valle, Toledo, Spain Spanish Network on Mastocytosis REMA
    PLoS ONE 8:e76116. 2013
    Serum baseline tryptase (sBT) is a minor diagnostic criterion for systemic mastocytosis (SM) of undetermined prognostic impact...
  76. pmc Characterizing roles for the glutathione reductase, thioredoxin reductase and thioredoxin peroxidase-encoding genes of Magnaporthe oryzae during rice blast disease
    Jessie Fernandez
    Department of Plant Pathology, University of Nebraska Lincoln, Lincoln, Nebraska, United States of America
    PLoS ONE 9:e87300. 2014
    ..oryzae sugar/ NADPH sensor Tps1, thereby suggesting how glucose availability, NADPH production and antioxidation might be connected...
  77. ncbi Mode of action of the qcr9 and cat3 mutations in restoring the ability of Saccharomyces cerevisiae tps1 mutants to grow on glucose
    M A Blazquez
    Unidad de Bioquímica y Genética de Levaduras, Instituto de Investigaciones Biomédicas del C S I C, Madrid, Spain
    Mol Gen Genet 249:655-64. 1995
    Mutations in the TPS1 gene, which encodes trehalose-6-P synthase, cause a glucose-negative phenotype in Saccharomyces cerevisiae...
  78. ncbi Sphingolipids are potential heat stress signals in Saccharomyces
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:30196-200. 1997
    ..Treatment of cells with dihydrosphingosine activates transcription of the TPS2 gene encoding a subunit of trehalose synthase and causes trehalose, a known thermoprotectant, to accumulate...
  79. pmc Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiae
    E Boy-Marcotte
    Institut de Genetique et Microbiologie, Unité de Recherche Associée CNRS 2225, Universite Paris Sud, Orsay, France
    J Bacteriol 180:1044-52. 1998
    ..Thirty-nine gene products (including those of ALD3, GDH3, GLK1, GPP2, HSP104, HXK1, PGM2, SOD2, SSA3, SSA4, TKL2, TPS1, and YBR149W) are dependent upon Msn2/4p for their induction at the diauxic transition...
  80. ncbi Trehalose-6P synthase is essential for trehalase activation triggered by glucose, nitrogen source or heat shock, but not by osmostress, in Schizosaccharomyces pombe
    J Cansado
    Department of Genetics and Microbiology, University of Murcia, 30071 Murcia, Spain
    Biochim Biophys Acta 1381:271-8. 1998
    Cells of Schizosaccharomyces pombe disrupted in the tps1+ gene, which encodes trehalose-6P synthase, were unable to increase trehalase activity in response to the addition of glucose or nitrogen source...
  81. ncbi Stimulation of proteinase activated receptor-2 causes endothelial cells to promote blood coagulation in vitro
    A K Alm
    Department of Physiology and Neuroscience, Lund University, Sweden
    Thromb Haemost 81:984-8. 1999
    ..this growing receptor subclass, can be activated by trypsin and trypsin-like serine proteases such as mast cell tryptase. PAR-2 is expressed in endothelial cells...
  82. ncbi Delayed onset of inflammation in protease-activated receptor-2-deficient mice
    J R Lindner
    Cardiovascular Division and Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Immunol 165:6504-10. 2000
    ..We hypothesized that activation of endothelial PAR2 by mast cell tryptase or other proteases also contributes to inflammatory responses...
  83. pmc Glycosylation and the activation of proteinase-activated receptor 2 (PAR(2)) by human mast cell tryptase
    S J Compton
    Diabetes and Endocrine Research Group, Department of Pharmacology and Therapeutics University of Calgary, Faculty of Medicine, Calgary, AB, Canada
    Br J Pharmacol 134:705-18. 2001
    1. Human mast cell tryptase appears to display considerable variation in activating proteinase-activated receptor 2 (PAR(2))...
  84. ncbi Functional effects of protease-activated receptor-2 stimulation on human airway smooth muscle
    L S Chambers
    Department of Pharmacology, University of Sydney, New South Wales 2006, Australia
    Am J Physiol Lung Cell Mol Physiol 281:L1369-78. 2001
    ..PAR)-2 is present on the smooth muscle and epithelium of human airways and can be activated by mast cell tryptase, trypsin, or the PAR-2 activating peptide (AP)...
  85. ncbi Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe
    Teresa Soto
    Department of Genetics and Microbiology, Facultad de Biologia, University of Murcia, Spain
    Eur J Biochem 269:3847-55. 2002
    ..we show that the products of the principal genes involved in trehalose metabolism in Schizosaccharomyces pombe, tps1+ (coding for trehalose-6-P synthase, Tps1p), ntp1+ (encoding neutral trehalase, Ntp1p) and tpp1+ (that codes for ..
  86. pmc Gts1p stabilizes oscillations in energy metabolism by activating the transcription of TPS1 encoding trehalose-6-phosphate synthase 1 in the yeast Saccharomyces cerevisiae
    Zhaojun Xu
    Department of Biochemistry 2, University of Yamanashi, Faculty of Medicine, 1110 Shimokato, Tamaho, Yamanashi 409 3898, Japan
    Biochem J 383:171-8. 2004
    ..Further, the expression of TPS1, encoding trehalose-6-phosphate synthase 1, and HSP104 was activated by Gts1p in combination with Snf1 kinase, a ..
  87. ncbi Tryptase activates phosphatidylinositol 3-kinases proteolytically independently from proteinase-activated receptor-2 in cultured dog airway smooth muscle cells
    James K Brown
    Pulmonary and Critical Care Medicine Section, Dept of Veterans Affairs Medical Center, and Department of Medicine, University of California San Francisco, CA 94121, USA
    Am J Physiol Lung Cell Mol Physiol 290:L259-69. 2006
    Mast cell tryptase is a potent mitogen for many cells in the airways and lung, but the cellular mechanisms for its growth stimulatory effects are poorly understood...
  88. pmc Degranulating mast cells in fibrotic regions of human tumors and evidence that mast cell heparin interferes with the growth of tumor cells through a mechanism involving fibroblasts
    Michael Samoszuk
    Department of Pathology and Laboratory Medicine, University of California, Irvine, California, USA
    BMC Cancer 5:121. 2005
    ..The purpose of this study was to test the hypothesis that mast cells that are present in fibrotic regions of cancer can suppress the growth of tumor cells through an indirect mechanism involving peri-tumoral fibroblasts...
  89. pmc WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis
    Ballachanda N Devaiah
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907 1165, USA
    Plant Physiol 143:1789-801. 2007
    ..revealed that the expression of several genes involved in Pi starvation responses, including phosphatases, Mt4/TPS1-like genes, and high-affinity Pi transporters, was decreased when WRKY75 was suppressed...
  90. ncbi Adaptive evolution of metabolic pathways in Drosophila
    J M Flowers
    Department of Ecology and Evolution, Stony Brook University, NY, USA
    Mol Biol Evol 24:1347-54. 2007
    ..evolution (glucose-6-phosphate dehydrogenase [G6pd]), phosphoglucomutase [Pgm], trehalose-6-phosphate synthetase [Tps1], and glucose-6phosphatase [G6pase] encode G6P branch point enzymes that catalyze reactions at the entry point to ..
  91. ncbi Trehalose is an important mediator of Cap1p oxidative stress response in Candida albicans
    Yingying Cao
    School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China
    Biol Pharm Bull 31:421-5. 2008
    ..Real time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcript levels of TPS2 and TPS3 were increased in the wild type strain compared to that in cap1/cap1 mutant when exposed to H2O2...
  92. ncbi Spontaneous cutaneous mast cell tumor with lymph node metastasis in a Richardson's ground squirrel (Spermophilus richardsonii)
    Xi Jun He
    Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    J Vet Diagn Invest 21:156-9. 2009
    ..The results of immunohistochemistry indicated positive labeling for vimentin, mast cell tryptase, c-kit, and Ki-67. Toluidine blue stain revealed fine, metachromatic, cytoplasmic granules...
  93. doi Investigation of the inflammatory mechanisms in alopecia areata
    Emel Dikicioglu Cetin
    Department of Pathology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey
    Am J Dermatopathol 31:53-60. 2009
    ..immunohistochemically with antibodies for CD1a, CD3, CD4, CD8, CD20, CD57 (for natural killer cells), mast cell tryptase, nerve growth factor receptor (NGFR), and substance P (SP). Various clinical findings were recorded...
  94. doi Correlation of plasma complement split product levels with allergic respiratory disease activity and relation to allergen immunotherapy
    Corinna Bowser
    Department of Medicine, State University of New York SUNY Downstate Medical Center, Brooklyn, New York 11203, USA
    Ann Allergy Asthma Immunol 104:42-9. 2010
    Allergens, including dust mite and grass pollen, and mast cell tryptase are known to generate the complement split products (CSPs) C5a and C3a, which can then trigger allergic inflammation...
  95. ncbi [Cloning and functional analysis of trehalose -6-phosphate synthase gene from Selaginella pulvinata]
    Jing Lin
    Maize Research Institute, Sichuan Agricultural University, Ya an 625014, China
    Yi Chuan 32:498-504. 2010
    ..Yeast functional complementation test showed that trehalose-6-phosphate synthase mutant (tps1 big up tri, open), transformed by the ORF of SpTPS1 gene, can restore growth on the medium supplemented with ..
  96. doi Chronological assessment of mast cell-mediated gut dysfunction and mucosal inflammation in a rat model of chronic psychosocial stress
    Maria Vicario
    Digestive Diseases Research Unit, Lab Neuro Immuno Gastroenterology, Institut de Recerca Vall d Hebron, CIBERehd, Department of Gastroenterology, Barcelona, Spain
    Brain Behav Immun 24:1166-75. 2010
    ..The notable resemblance of these findings with those in some IBS patients, support the potential interest and translational validity of this experimental model for the research of stress-sensitive intestinal disorders, particularly IBS...
  97. doi Mast cell tryptase stimulates production of decorin by human testicular peritubular cells: possible role of decorin in male infertility by interfering with growth factor signaling
    M Adam
    Anatomy and Cell Biology, Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, D 80802 Munich, Germany
    Hum Reprod 26:2613-25. 2011
    ..In men with impaired spermatogenesis, fibrotic remodeling of these walls and accumulation of tryptase-positive mast cells (MCs) occur.
  98. pmc Honeybee venom immunotherapy: a comparative study using purified and nonpurified aqueous extracts in patients with normal Basal serum tryptase concentrations
    M Beatrice Bilò
    Allergy Unit, Department of Allergy, Immunology and Respiratory Diseases, University Hospital Ospedali Riuniti, Via Conca 1, 60020 Ancona, Italy
    J Allergy (Cairo) 2012:869243. 2012
    ..preparation under the same build-up protocol in bee venom allergic patients with a normal baseline mast cell tryptase concentration...
  99. doi Usefulness and limitations of sequential serum tryptase for the diagnosis of anaphylaxis in 102 patients
    Anna Sala-Cunill
    Allergy Section, Internal Medicine Department, Hospital Universitari Vall d Hebron, Universitat Autonoma de Barcelona, Barcelona, Spain
    Int Arch Allergy Immunol 160:192-9. 2013
    ..The diagnosis of anaphylaxis is based on clinical history since no reliable biological marker is currently available to confirm the diagnosis...
  100. doi Mast cell tryptase levels in gut mucosa in patients with gastrointestinal symptoms caused by food allergy
    A F Hagel
    Department of Medicine I, Functional Tissue Diagnostics, University Erlangen Nuremberg, Erlangen, Germany
    Int Arch Allergy Immunol 160:350-5. 2013
    ..The objective of this study was to investigate whether mast cell tryptase (T), a typical mast cell-associated marker, may help to detect patients with food allergy.
  101. doi Critical role of protease-activated receptor 2 activation by mast cell tryptase in the development of postoperative pain
    Sara M Oliveira
    Department of Chemistry, Graduate Program in Biological Sciences Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil
    Anesthesiology 118:679-90. 2013
    ..Thus, the aim of this study was to investigate the involvement of mast cell degranulation, tryptase and its substrate, the protease-activated receptor 2, in a model of postoperative pain in mice.

Research Grants19

  1. Praveen Thumbikat; Fiscal Year: 2016
    ..We will perform rigorous clinical studies to validate the use of MCP-1 and MIP-11 and mast cell tryptase as biomarkers for CPPS in humans and correlate their levels with CPPS symptoms...
  2. Matthew J Hamilton; Fiscal Year: 2016
    ..The human ortholog to mMCP-6 is Tryptase-[unreadable]1 which is the dominant mast cell tryptase in humans...
  3. RECOMBINANT HUMAN MAST CELL TRYPTASES
    David Johnson; Fiscal Year: 2000
    ..In preliminary studies the principal investigator has expressed and purified fully active recombinant human skin tryptase 1 (rHST1) in Pichia pastoris...
  4. Kalpna Gupta; Fiscal Year: 2016
    ..In turn, mast cell tryptase activates protease activated receptor 2 (PAR 2) on sensory nerve endings maintaining nociceptor sensitization and ..
  5. PLA2 activation by mast cell tryptase in IC
    Jane McHowat; Fiscal Year: 2007
    ..We hypothesize that these two processes participate in a vicious cycle of inflammation, with tryptase released from activated mast cells activating the protease-activated receptor-2 (PAR-2) on urothelial and ..
  6. MOLECULAR PATHOGENESIS OF RADIATION ENTEROPATHY
    Martin Hauer Jensen; Fiscal Year: 2005
    ..appear to be mutually dependent; that protease-activated receptor 2 (PAR-2), which is activated by mast cell tryptase and mediates mitogenic and fibrogenic responses, is upregulated in intestinal radiation fibrosis; and that ..
  7. PAR2, Bronchopulmonary Sensory Nerve and Pathogenesis of Asthma
    Qihai Gu; Fiscal Year: 2009
    ..Our hypothesis is that when PAR2 is activated by endogenous (e.g., mast cell tryptase and trypsin-like proteases) or exogenous (e.g...
  8. BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMES
    Lawrence Schwartz; Fiscal Year: 2000
    ..The major component of the secretory granules of all human mast cells on a weight basis is the tryptase family of serine proteases...
  9. MECHANISMS AND MODULATION OF VISCERAL PAIN
    Gerald Gebhart; Fiscal Year: 2009
    ..We also hypothesize that protons and/or endogenous mediators (e.g., ATP, mast cell tryptase, serotonin) contribute to visceral hypersensitivity and can do so in the absence of frank tissue damage, which is ..
  10. EFFECTS OF O3 AND NO2 ON HUMAN LUNG PROTEINS
    David Johnson; Fiscal Year: 1992
    ..b>Tryptase is the principal granular protein of human mast cells...
  11. THE ROLE OF CTGF IN SUBEPITHELIAL FIBROSIS IN ASTHMA
    DAVID RISHIKOF; Fiscal Year: 2004
    ..Preliminary data indicate that the cell products TGF-beta and IL-4, and trypsin (a protease related to mast cell tryptase) induce CTGF and type I collagen in fibroblasts...
  12. BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMES
    Lawrence Schwartz; Fiscal Year: 2005
    ..The major component of the secretory granules of all human mast cells on a weight basis is the tryptase family of serine proteases...
  13. Flourescent Ceramic Nanoprobes (RMI)
    TIMOTHY BOYLE; Fiscal Year: 2008
    ..All of the innovations uncovered during the preparation of these nanoprobes will be shared in order to benefit medical research applications beyond those proposed in this investigation. ..
  14. CHARACTERIZATION OF DIFFERENT TYPES OF HUMAN MAST CELLS
    Lawrence Schwartz; Fiscal Year: 2009
    ..Aim 1 examines new functional and phenotypic characteristics that distinguish MCT (tryptase+/chymase-) from MCTC (tryptase+/chymase-)types of human mast cells...
  15. MECHANISM OF HUMAN MAST CELL TRYPTASE REGULATION
    Norman Schechter; Fiscal Year: 2003
    ..of this work is to describe the biochemical mechanism for the functional instability of the serine protease human tryptase and to define the relevance of this mechanism to the regulation of tryptase in vivo...
  16. SERINE PROTEASES FROM MAST CELLS AND SKIN
    Norman Schechter; Fiscal Year: 2002
    ..It is hoped that these studies will advance our understanding of two classes of molecules which are important to production and regulation of inflammation. ..
  17. EVALUATION OF BASOPHIL INVOLVEMENT IN HUMAN DISEASE
    Lawrence Schwartz; Fiscal Year: 2001
    ..In humans, mast cell involvement can be precisely identified by the presence of tryptase. For basophils, no such direct marker has been identified...