TRIM5alpha

Summary

Gene Symbol: TRIM5alpha
Description: tripartite motif containing 5
Alias: RNF88, TRIM5alpha, tripartite motif-containing protein 5, RING-type E3 ubiquitin transferase TRIM5, ring finger protein 88, tripartite motif containing 5 transcript variant iota, tripartite motif containing 5 transcript variant kappa, tripartite motif protein TRIM5
Species: human

Top Publications

  1. doi Innate signaling in HIV-1 infection of dendritic cells
    Michiel van der Vlist
    Center for Experimental and Molecular Medicine and Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Curr Opin HIV AIDS 6:348-52. 2011
  2. doi Immune evasion and counteraction of restriction factors by HIV-1 and other primate lentiviruses
    Frank Kirchhoff
    Institute of Molecular Virology, University Hospital Ulm, Meyerhofstrasse 1, 89081 Ulm, Germany
    Cell Host Microbe 8:55-67. 2010
  3. pmc The TRIM5alpha B-box 2 domain promotes cooperative binding to the retroviral capsid by mediating higher-order self-association
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 82:11495-502. 2008
  4. pmc TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
    Zana Lukic
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, USA
    Retrovirology 8:93. 2011
  5. pmc Gag cytotoxic T lymphocyte escape mutations can increase sensitivity of HIV-1 to human TRIM5alpha, linking intrinsic and acquired immunity
    Emilie Battivelli
    INSERM U941, Hopital Saint Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    J Virol 85:11846-54. 2011
  6. pmc Contribution of E3-ubiquitin ligase activity to HIV-1 restriction by TRIM5alpha(rh): structure of the RING domain of TRIM5alpha
    Maritza Lienlaf
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 85:8725-37. 2011
  7. pmc TRIM5alpha disrupts the structure of assembled HIV-1 capsid complexes in vitro
    LESA R BLACK
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    J Virol 84:6564-9. 2010
  8. pmc Biochemical characterization of a recombinant TRIM5alpha protein that restricts human immunodeficiency virus type 1 replication
    Charles R Langelier
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112 5650, USA
    J Virol 82:11682-94. 2008
  9. pmc Second-site suppressors of HIV-1 capsid mutations: restoration of intracellular activities without correction of intrinsic capsid stability defects
    Ruifeng Yang
    Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Retrovirology 9:30. 2012
  10. pmc Adaptation of HIV-1 to cells expressing rhesus monkey TRIM5α
    Beatriz Pacheco
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Virology 408:204-12. 2010

Research Grants

  1. Joseph G Sodroski; Fiscal Year: 2014
  2. Optimized Adaptation of Simian-tropic R5 HIV Clade C to Pig-tailed Macaques
    RUTH MARGRIT RUPRECHT; Fiscal Year: 2013
  3. The Role of CyclophilinA in the Evolution of Retroviral Restriction
    RAYMOND MALFAVON BORJA; Fiscal Year: 2010
  4. Felipe Diaz-Griffero; Fiscal Year: 2016
  5. Paul D Bieniasz; Fiscal Year: 2016
  6. The role of UBE2V1 in HIV-1 restriction in primary monocytes
    Li Wu; Fiscal Year: 2013
  7. Structural Basis of Retroviral Restriction by TRIM5alpha
    DMITRI N IVANOV; Fiscal Year: 2010
  8. Regulators and mediators of TRIM5 function
    Jacek Skowronski; Fiscal Year: 2010
  9. HIV-1 Resistance to Type I Interferon in Macaques
    Jason T Kimata; Fiscal Year: 2013
  10. Eric M Poeschla; Fiscal Year: 2016

Detail Information

Publications170 found, 100 shown here

  1. doi Innate signaling in HIV-1 infection of dendritic cells
    Michiel van der Vlist
    Center for Experimental and Molecular Medicine and Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Curr Opin HIV AIDS 6:348-52. 2011
    ..This review summarizes the current knowledge of innate signaling events that are involved in HIV-1 infection. We here focus on dendritic cells, which are among the first cells that encounter HIV-1 after exposure...
  2. doi Immune evasion and counteraction of restriction factors by HIV-1 and other primate lentiviruses
    Frank Kirchhoff
    Institute of Molecular Virology, University Hospital Ulm, Meyerhofstrasse 1, 89081 Ulm, Germany
    Cell Host Microbe 8:55-67. 2010
    ....
  3. pmc The TRIM5alpha B-box 2 domain promotes cooperative binding to the retroviral capsid by mediating higher-order self-association
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 82:11495-502. 2008
    The retroviral restriction factor, TRIM5alpha, blocks infection of a spectrum of retroviruses soon after virus entry into the cell. TRIM5alpha consists of RING, B-box 2, coiled-coil, and B30.2(SPRY) domains...
  4. pmc TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
    Zana Lukic
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, USA
    Retrovirology 8:93. 2011
    ..To better understand the mechanism of restriction, we conducted yeast-two hybrid screens to identify proteins that bind to two TRIM5 orthologues...
  5. pmc Gag cytotoxic T lymphocyte escape mutations can increase sensitivity of HIV-1 to human TRIM5alpha, linking intrinsic and acquired immunity
    Emilie Battivelli
    INSERM U941, Hopital Saint Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    J Virol 85:11846-54. 2011
    ..Taken together, these findings suggest that CTL mutations may influence HIV-1 replication by modifying both viral infectivity and sensitivity to TRIM5α...
  6. pmc Contribution of E3-ubiquitin ligase activity to HIV-1 restriction by TRIM5alpha(rh): structure of the RING domain of TRIM5alpha
    Maritza Lienlaf
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 85:8725-37. 2011
    ..Remarkably, all of these changes were in residues located in the E2-binding region of the RING domain. Overall, these results demonstrate a role for TRIM5α self-ubiquitylation in the ability of TRIM5α to restrict HIV-1...
  7. pmc TRIM5alpha disrupts the structure of assembled HIV-1 capsid complexes in vitro
    LESA R BLACK
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    J Virol 84:6564-9. 2010
    The host restriction factor TRIM5alpha provides intrinsic defense against retroviral infections in mammalian cells...
  8. pmc Biochemical characterization of a recombinant TRIM5alpha protein that restricts human immunodeficiency virus type 1 replication
    Charles R Langelier
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112 5650, USA
    J Virol 82:11682-94. 2008
    The rhesus monkey intrinsic immunity factor TRIM5alpha(rh) recognizes incoming capsids from a variety of retroviruses, including human immunodeficiency virus type 1 (HIV-1) and equine infectious anemia virus (EIAV), and inhibits the ..
  9. pmc Second-site suppressors of HIV-1 capsid mutations: restoration of intracellular activities without correction of intrinsic capsid stability defects
    Ruifeng Yang
    Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Retrovirology 9:30. 2012
    ..Based on previous studies of HIV-1 CA mutants exhibiting altered capsid stability, we concluded that formation of a capsid of optimal intrinsic stability is crucial for HIV-1 infection...
  10. pmc Adaptation of HIV-1 to cells expressing rhesus monkey TRIM5α
    Beatriz Pacheco
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Virology 408:204-12. 2010
    ..Thus, a preferred HIV-1 escape pathway involves decreased binding to TRIM5α, a capsid-destabilizing factor, and retention of binding to cyclophilin A, a capsid-stabilizing factor...
  11. pmc Primate lentivirus capsid sensitivity to TRIM5 proteins
    Zerina Kratovac
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, NY 10016, USA
    J Virol 82:6772-7. 2008
    ..b>TRIM5alpha proteins from other primates exhibited distinct restriction specificities for primate lentivirus capsids...
  12. pmc A rare null allele potentially encoding a dominant-negative TRIM5alpha protein in Baka pygmies
    Judith N Torimiro
    Department of Biochemistry and Physiologic Sciences, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
    Virology 391:140-7. 2009
    ..b>TRIM5alpha is a restriction factor that can decrease the susceptibility of cells of particular mammalian species to ..
  13. doi An HIV-1 resistance polymorphism in TRIM5α gene among Chinese intravenous drug users
    Feng Liang Liu
    Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China
    J Acquir Immune Defic Syndr 56:306-11. 2011
    ..Previous association studies suggested that polymorphisms in TRIM5α gene might protect against HIV-1 infection. However, the exact variation accounting for this protective effect was not certain...
  14. pmc Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms
    Emilie Battivelli
    INSERM U941, Paris 75010, France
    J Virol 85:7828-35. 2011
    ..Specific knockdown of TRIM5ι increases TRIM5α activity in human U373-X4 cells, indicating that physiological levels of expression of truncated TRIM5 isoforms in human cells can reduce the activity of TRIM5α...
  15. doi Targeting HIV-1 innate immune responses therapeutically
    Rada Ellegård
    Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden
    Curr Opin HIV AIDS 6:435-43. 2011
    ..This review addresses the recent progress in the understanding of innate immune responses against HIV-1 with focus on the potential targets for prevention of viral acquisition, replication and dissemination...
  16. pmc The structural biology of HIV-1: mechanistic and therapeutic insights
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    Nat Rev Microbiol 10:279-90. 2012
    ..Here, we review recent advances in HIV-1 structural biology, focusing on the molecular mechanisms of viral replication and on the development of new therapeutics...
  17. pmc Trim5alpha protein restricts both HIV-1 and murine leukemia virus
    Melvyn W Yap
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Proc Natl Acad Sci U S A 101:10786-91. 2004
    ..Recently, Trim5alpha protein from rhesus monkey was found to restrict HIV-1...
  18. pmc Strain-specific differences in the impact of human TRIM5alpha, different TRIM5alpha alleles, and the inhibition of capsid-cyclophilin A interactions on the infectivity of HIV-1
    Emilie Battivelli
    INSERM U941, Institut Universitaire d Hematologie, Universite Paris Diderot, Hopital Saint Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    J Virol 84:11010-9. 2010
    ..Taken together, these findings support a model in which three interlinked factors--capsid sequence, CypA levels, and TRIM5α--interact to determine capsid stability and therefore viral infectivity...
  19. pmc Implication of TRIM alpha and TRIMCyp in interferon-induced anti-retroviral restriction activities
    Laetitia Carthagena
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Departement des Maladies Infectieuses, 22 rue Mechain, 75014, Paris, France
    Retrovirology 5:59. 2008
    ..We investigated the consequences of IFN treatment on retroviral restriction in diverse primate cells and evaluated the implication of TRIM5 alpha or TRIMCyp in IFN-induced anti-retroviral activities...
  20. pmc Cyclophilin A, TRIM5, and resistance to human immunodeficiency virus type 1 infection
    Jeremy Luban
    Institute for Research in Biomedicine, Via Vincenzo Vela 6, CH 6500 Bellinzona, Switzerland
    J Virol 81:1054-61. 2007
  21. pmc The human and African green monkey TRIM5alpha genes encode Ref1 and Lv1 retroviral restriction factor activities
    Zuzana Keckesova
    Wohl Virion Center, Division of Infection and Immunity, University College London, London W1T 4JF, United Kingdom
    Proc Natl Acad Sci U S A 101:10780-5. 2004
    The rhesus macaque tripartite motif containing protein TRIM5alpha specifically restricts HIV-1 infection at an early post-entry step before reverse transcription [Stremlau, M., Owens, C. M., Perron, M. J., Kiessling, M., Autissier, P...
  22. ncbi Rapid turnover and polyubiquitylation of the retroviral restriction factor TRIM5
    Felipe Diaz-Griffero
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Department of Pathology, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 349:300-15. 2006
    b>TRIM5alpha and TRIMCyp are retroviral restriction factors that, like other members of the tripartite motif (TRIM) family, contain RING, B-box 2 and coiled-coil domains...
  23. ncbi Saturation of TRIM5 alpha-mediated restriction of HIV-1 infection depends on the stability of the incoming viral capsid
    Jiong Shi
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    Virology 350:493-500. 2006
    ..The results strongly suggest that the mechanism of HIV-1 restriction by TRIM5 alpha involves engagement of the viral capsid by the restriction factor prior to completion of uncoating...
  24. ncbi Cyclophilin A: an auxiliary but not necessary cofactor for TRIM5alpha restriction of HIV-1
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 351:112-20. 2006
    ..The retroviral restriction factor TRIM5alpha can inhibit the early, post-entry phase of infection by associating with the incoming viral capsid...
  25. pmc Interfering residues narrow the spectrum of MLV restriction by human TRIM5alpha
    Pierre V Maillard
    Global Health Institute, School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    PLoS Pathog 3:e200. 2007
    b>TRIM5alpha is a restriction factor that limits infection of human cells by so-called N- but not B- or NB-tropic strains of murine leukemia virus (MLV)...
  26. pmc Somatic cell genetic analyses to identify HIV-1 host restriction factors
    Susana T Valente
    Department of Biochemistry, Columbia University, Howard Hughes Medical Institute College of Physicians and Surgeons, New York NY, USA
    Methods Mol Biol 485:235-55. 2009
    ..Here we report the strategy and techniques to prepare a library and isolate HIV antiviral genes, using the identification of N-86-HnRNPU as an example...
  27. doi The capsid protein of human immunodeficiency virus: interactions of HIV-1 capsid with host protein factors
    Anjali P Mascarenhas
    Department of Chemistry, The Ohio State University, Columbus, OH, USA
    FEBS J 276:6118-27. 2009
    ..These interactions and their characterization present novel frontiers for the design and advancement of antiviral therapeutics...
  28. doi A new functional role of HIV-1 integrase during uncoating of the viral core
    Marisa S Briones
    Department of Molecular and Medical Pharmacology, Molecular Biology Institute, 650 Charles E Young Drive, Los Angeles, CA 90095, USA
    Immunol Res 48:14-26. 2010
    ..Cyclophilin A and TRIM5α are two cellular factors that interact with CA in exerting their effects on HIV-1 replication. Here, we review the current understanding of uncoating and the new functional role of HIV-1 IN during uncoating...
  29. doi Human immunodeficiency virus type 1 restriction by human-rhesus chimeric tripartite motif 5alpha (TRIM 5alpha) in CD34(+) cell-derived macrophages in vitro and in T cells in vivo in severe combined immunodeficient (SCID-hu) mice transplanted with human fe
    Joseph Anderson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Hum Gene Ther 19:217-28. 2008
    ..The retroviral restriction factor TRIM5alpha (tripartite motif 5alpha protein) has been shown to potently restrict human immunodeficiency virus (HIV)-1 ..
  30. pmc Human TRIM5alpha expression levels and reduced susceptibility to HIV-1 infection
    Shamman Sewram
    Nelson R Mandela School of Medicine, University of KwaZulu Natal, Durban, South Africa
    J Infect Dis 199:1657-63. 2009
    Human TRIM5alpha (TRIM5alphahu), a member of the tripartite motif protein family, displays some anti-human immunodeficiency virus type 1 (HIV-1) activity in vitro, although it is substantially less potent than its rhesus monkey ..
  31. doi Impact of novel TRIM5alpha variants, Gly110Arg and G176del, on the anti-HIV-1 activity and the susceptibility to HIV-1 infection
    Toshiaki Nakajima
    Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
    AIDS 23:2091-100. 2009
    b>TRIM5alpha is one of the factors contributing to intracellular defense mechanisms against HIV-1 infection. We investigated the association of TRIM5alpha sequence variations with the susceptibility to HIV-1 infection in Japanese and Indian.
  32. pmc Human TRIM5alpha mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants
    Patrick Kaumanns
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Retrovirology 3:79. 2006
    In order to characterize the antiviral activity of human TRIM5alpha in more detail human derived indicator cell lines over expressing wild type human TRIM5alpha were generated and challenged with HIV-1 and HIV-2 viruses pseudotyped with ..
  33. pmc Retroviral restriction factor TRIM5alpha is a trimer
    Claudia C Mische
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 79:14446-50. 2005
    The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30...
  34. pmc Structural and functional insights into the B30.2/SPRY domain
    Jae Sung Woo
    Division of Molecular and Life Sciences, Department of Life Sciences, Center for Biomolecular Recognition, Pohang University of Science and Technology, Pohang, Kyungbuk, Korea
    EMBO J 25:1353-63. 2006
    ..approximately 700 eukaryotic (approximately 150 human) proteins, including medically important proteins such as TRIM5alpha and Pyrin. Nonetheless, the functional role of this modular domain remained unclear...
  35. pmc Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components
    Martha R Neagu
    Department of Microbiology and Columbia University, New York, NY, USA
    J Clin Invest 119:3035-47. 2009
    ..The anti-HIV-1 activity of hT5Cyp was surprisingly more effective than that of the well-characterized rhesus TRIM5alpha, especially in T cells...
  36. pmc Live cell imaging of the HIV-1 life cycle
    Edward M Campbell
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Trends Microbiol 16:580-7. 2008
    ..In this review, we discuss how researchers have exploited fluorescent microscopy methodologies to observe and quantify these events occurring during the replication of HIV-1 in living cells...
  37. pmc Functional replacement of the RING, B-box 2, and coiled-coil domains of tripartite motif 5alpha (TRIM5alpha) by heterologous TRIM domains
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street JFB 824, Boston, MA 02115, USA
    J Virol 80:6198-206. 2006
    Tripartite motif 5alpha (TRIM5alpha) restricts some retroviruses, including human immunodeficiency virus type 1 (HIV-1), from infecting the cells of particular species...
  38. ncbi Unique features of TRIM5alpha among closely related human TRIM family members
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 360:419-33. 2007
    The tripartite motif (TRIM) protein, TRIM5alpha, restricts some retroviruses, including human immunodeficiency virus (HIV-1), from infecting the cells of particular species...
  39. ncbi BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta
    Lixin Xu
    Department of Biochemistry and Molecular Biology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 4799, USA
    Exp Cell Res 288:84-93. 2003
    ..Thus, four protein modules found on each of these putative ubiquitin ligases, a RING, a B-box and two PHR repeats, are present on BTBD1/2 and TRIM5delta that are colocalized to cytoplasmic bodies...
  40. ncbi Adaptive evolution of primate TRIM5alpha, a gene restricting HIV-1 infection
    Hong Liang Liu
    Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan, China
    Gene 362:109-16. 2005
    Recent studies showed that nonhuman primate TRIM5alpha can efficiently block HIV-1 infection in human cell lines. It can also restrict other retroviruses, therefore, suggested as a general defender against retrovirus infection...
  41. ncbi A retrovirus restriction factor TRIM5alpha is transcriptionally regulated by interferons
    Kentaro Asaoka
    Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan
    Biochem Biophys Res Commun 338:1950-6. 2005
    b>TRIM5alpha is a member of tripartite motif protein family and recently identified as a restriction factor for retroviral infection in a species-specific manner...
  42. ncbi Antiretroviral potential of human tripartite motif-5 and related proteins
    Fengwen Zhang
    Aaron Diamond AIDS Research Center and the Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, NY 10016, USA
    Virology 353:396-409. 2006
    b>TRIM5alpha is a potent inhibitor of infection by diverse retroviruses and is encoded by one of a large family of TRIM genes...
  43. ncbi Trim5alpha accelerates degradation of cytosolic capsid associated with productive HIV-1 entry
    Udayan Chatterji
    Departments of Immunology, Molecular and Integrative Neurosciences, and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 281:37025-33. 2006
    The TRIM5alpha (tripartite motif 5alpha protein) has been linked to the cross-species restriction in human immunodeficiency virus type 1 (HIV-1) infection of non-human cells, but the mechanism by which this occurs remains to be fully ..
  44. pmc The cell biology of HIV-1 and other retroviruses
    Eric O Freed
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
    Retrovirology 3:77. 2006
    ..MEETING REPORT: The conference began with a keynote address from W. Sundquist on the biochemistry of HIV-1 budding. This presentation will be described in the section on Assembly and Release of Retroviruses...
  45. pmc Alpha interferon enhances TRIM5alpha-mediated antiviral activities in human and rhesus monkey cells
    Ryuta Sakuma
    Molecular Medicine Program, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    J Virol 81:10201-6. 2007
    Dominant, constitutively expressed antiretroviral factors, including TRIM5alpha and APOBEC3 proteins, are distinguished from the conventional innate immune systems and are classified as intrinsic immunity factors...
  46. doi Evasion from CypA- and APOBEC-mediated restrictions is insufficient for HIV-1 to efficiently grow in simian cells
    Kazuya Kamada
    Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770 8503, Japan
    Microbes Infect 11:164-71. 2009
    ..Consistently, any of the HIV-1 variants examined here did not effectively counter CyM TRIM5alpha as judged by single-cycle infectivity assays...
  47. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
    ..Here we identify TRIM5alpha, a component of cytoplasmic bodies, as the blocking factor...
  48. pmc Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha
    Theodora Hatziioannou
    Aaron Diamond AIDS Research Center, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 101:10774-9. 2004
    ..susceptibility factor 1 and Ref1 are species-specific variants of tripartite interaction motif 5alpha (TRIM5alpha), a cytoplasmic body component recently shown to block HIV-1 infection in rhesus macaque cells, and can indeed ..
  49. pmc TRIM5alpha mediates the postentry block to N-tropic murine leukemia viruses in human cells
    Michel J Perron
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:11827-32. 2004
    ..A similar block to HIV-1 in rhesus monkey cells is mediated by TRIM5alpha. Here we show that human TRIM5alpha is both necessary and sufficient for the restriction of N-MLV in human cells...
  50. ncbi A single amino acid change in the SPRY domain of human Trim5alpha leads to HIV-1 restriction
    Melvyn W Yap
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Curr Biol 15:73-8. 2005
    ..Recently, it has become clear that Ref1 and Lv1 are encoded by the same gene, Trim5alpha, which inhibits retrovirus replication in a species-specific manner...
  51. pmc Species-specific variation in the B30.2(SPRY) domain of TRIM5alpha determines the potency of human immunodeficiency virus restriction
    Matthew Stremlau
    Dana Farber Cancer Institute, 44 Binney St, JFB 824, Boston, MA 02115, USA
    J Virol 79:3139-45. 2005
    ..Human immunodeficiency virus type 1 (HIV-1) is blocked in the cells of Old World monkeys by TRIM5alpha, a tripartite motif (TRIM) protein composed of RING, B-box 2, coiled-coil, and B30.2(SPRY) domains...
  52. pmc Retrovirus restriction by TRIM5alpha variants from Old World and New World primates
    Byeongwoon Song
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA, USA
    J Virol 79:3930-7. 2005
    The TRIM5alpha proteins of humans and some Old World monkeys have been shown to block infection of particular retroviruses following virus entry into the host cell...
  53. pmc Disruption of human TRIM5alpha antiviral activity by nonhuman primate orthologues
    Lionel Berthoux
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th St, New York, NY 10032, USA
    J Virol 79:7883-8. 2005
    ..Human immunodeficiency virus type 1 (HIV-1) infection is blocked by the alpha isoform of macaque TRIM5 (TRIM5alpha(rh)) or by the product of the owl monkey TRIM5-cyclophilin A gene fusion (TRIMCyp)...
  54. pmc TRIM5alpha selectively binds a restriction-sensitive retroviral capsid
    Sarah Sebastian
    Department of Microbiology, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, HHSC 1502, New York, New York 10032, USA
    Retrovirology 2:40. 2005
    ..Here we show that TRIM5alpha binds retroviral CA from detergent-stripped virions in a SPRY-dependent manner with sufficient discrimination to ..
  55. pmc Human tripartite motif 5alpha domains responsible for retrovirus restriction activity and specificity
    David Perez-Caballero
    Aaron Diamond AIDS Research Center, 455 First Ave, New York, NY 10021, USA
    J Virol 79:8969-78. 2005
    The tripartite motif 5alpha protein (TRIM5alpha) is one of several factors expressed by mammalian cells that inhibit retrovirus replication...
  56. ncbi High-frequency persistence of an impaired allele of the retroviral defense gene TRIM5alpha in humans
    Sara L Sawyer
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Curr Biol 16:95-100. 2006
    The intracellular TRIM5alpha protein successfully inhibits HIV-1 infection in rhesus monkeys, but not in humans ...
  57. pmc Retroviral restriction factors Fv1 and TRIM5alpha act independently and can compete for incoming virus before reverse transcription
    Luca D Passerini
    Wohl Virion Centre, Division of Infection and Immunity, Royal Free and University College Medical School, University College London, UK
    J Virol 80:2100-5. 2006
    The restriction factors Fv1 and TRIM5alpha provide dominant blocks to retroviral infection, targeting incoming capsids at a postentry, preintegration step...
  58. pmc Genetic association of the antiviral restriction factor TRIM5alpha with human immunodeficiency virus type 1 infection
    Emily C Speelmon
    Medical Scientist Training Program, Molecular and Cellular Biology Program, University of Washington, Seattle, 98109, USA
    J Virol 80:2463-71. 2006
    The innate antiviral factor TRIM5alpha restricts the replication of some retroviruses through its interaction with the viral capsid protein, leading to abortive infection...
  59. pmc Cyclophilin A and TRIM5alpha independently regulate human immunodeficiency virus type 1 infectivity in human cells
    Elena Sokolskaja
    Department of Microbiology, Columbia University, 701 West 168th Street, New York, New York 10032, USA
    J Virol 80:2855-62. 2006
    ..or African green monkey cells, where, paradoxically, the interaction decreases HIV-1 infectivity by facilitating TRIM5alpha-mediated restriction...
  60. ncbi Characterization of TRIM5alpha trimerization and its contribution to human immunodeficiency virus capsid binding
    Hassan Javanbakht
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 353:234-46. 2006
    The coiled-coil domain of the tripartite motif (TRIM) family protein TRIM5alpha is required for trimerization and function as an antiretroviral restriction factor...
  61. pmc Removal of arginine 332 allows human TRIM5alpha to bind human immunodeficiency virus capsids and to restrict infection
    Yuan Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, JFB 824, Boston, MA 02115, USA
    J Virol 80:6738-44. 2006
    Human TRIM5alpha (TRIM5alpha(hu)) only modestly inhibits human immunodeficiency virus type 1 (HIV-1) and does not inhibit simian immunodeficiency virus (SIV(mac)). Alteration of arginine 332 in the TRIM5alpha(hu) B30...
  62. ncbi Effects of human TRIM5alpha polymorphisms on antiretroviral function and susceptibility to human immunodeficiency virus infection
    Hassan Javanbakht
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 354:15-27. 2006
    b>TRIM5alpha acts on several retroviruses, including human immunodeficiency virus (HIV-1), to restrict cross-species transmission...
  63. pmc All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction
    Sadayuki Ohkura
    Division of Virology, National Institute for Medical Research, Medical Research Council, London, UK
    J Virol 80:8554-65. 2006
    Recent studies have revealed the contribution of TRIM5alpha to retrovirus restriction in cells from a variety of primate species. TRIM5alpha consists of a tripartite motif (the RBCC domain) followed by a B30.2 domain. The B30...
  64. pmc Proteasome inhibition reveals that a functional preintegration complex intermediate can be generated during restriction by diverse TRIM5 proteins
    Jenny L Anderson
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Ward 8 140, 303 East Chicago Avenue, Chicago, IL 60611, USA
    J Virol 80:9754-60. 2006
    ..We recently found that proteasome inhibitors altered the rhesus monkey TRIM5alpha restriction of human immunodeficiency virus type 1 (HIV-1), allowing reverse transcription products to ..
  65. pmc The human TRIM5alpha restriction factor mediates accelerated uncoating of the N-tropic murine leukemia virus capsid
    Michel J Perron
    Dana Farber Cancer Institute, 44 Binney Street, JFB 824, Boston, MA 02115, USA
    J Virol 81:2138-48. 2007
    The host cell factors TRIM5alpha(hu) and Fv-1 restrict N-tropic murine leukemia virus (N-MLV) infection at an early postentry step before or after reverse transcription, respectively...
  66. pmc TRIM5 alpha cytoplasmic bodies are highly dynamic structures
    Edward M Campbell
    Department of Cell and Molecular Biology, Northwestern University, Chicago, IL 60611 3008, USA
    Mol Biol Cell 18:2102-11. 2007
    ..Therefore, TRIM5 alpha cytoplasmic bodies are dynamic structures more consistent with a role in function or regulation rather than protein aggregates or inclusion bodies that represent dead-end static structures...
  67. ncbi Wild type and H43Y variant of human TRIM5alpha show similar anti-human immunodeficiency virus type 1 activity both in vivo and in vitro
    Emi E Nakayama
    Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Shi, Osaka, Japan
    Immunogenetics 59:511-5. 2007
    ..Recently, tripartite motif 5alpha (TRIM5alpha) was identified as a factor that confers resistance to HIV-1 infection in Old World monkey cells...
  68. pmc Functional interplay between the B-box 2 and the B30.2(SPRY) domains of TRIM5alpha
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 366:234-44. 2007
    The retroviral restriction factors, TRIM5alpha and TRIMCyp, consist of RING and B-box 2 domains separated by a coiled coil from carboxy-terminal domains. These carboxy-terminal domains (the B30...
  69. ncbi Restriction of an extinct retrovirus by the human TRIM5alpha antiviral protein
    Shari M Kaiser
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Science 316:1756-8. 2007
    ..endogenous virus from the chimpanzee genome and show that the human variant of the intrinsic immune protein TRIM5alpha can actively prevent infection by this virus...
  70. pmc Modulation of retroviral restriction and proteasome inhibitor-resistant turnover by changes in the TRIM5alpha B-box 2 domain
    Felipe Diaz-Griffero
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Virol 81:10362-78. 2007
    An intact B-box 2 domain is essential for the antiretroviral activity of TRIM5alpha. We modeled the structure of the B-box 2 domain of TRIM5alpha based on the existing three-dimensional structure of the B-box 2 domain of human TRIM29...
  71. pmc An active TRIM5 protein in rabbits indicates a common antiviral ancestor for mammalian TRIM5 proteins
    Torsten Schaller
    MRC Centre for Medical Molecular Virology, Department of Infection, Royal Free and University College Medical School, University College London, 46 Cleveland Street, London, United Kingdom
    J Virol 81:11713-21. 2007
    ..Restriction is species specific. For example, TRIM5alpha from Old World monkeys, but not humans, restricts human immunodeficiency virus type 1 infection...
  72. pmc TRIM E3 ligases interfere with early and late stages of the retroviral life cycle
    Pradeep D Uchil
    Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America
    PLoS Pathog 4:e16. 2008
    ..In contrast, downregulation of TRIM11 and 15 enhanced virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells...
  73. pmc The effect of Trim5 polymorphisms on the clinical course of HIV-1 infection
    Danielle Van Manen
    Department of Clinical Viro Immunology, Sanquin Research, Landsteiner Laboratory, University of Amsterdam, Amsterdam, The Netherlands
    PLoS Pathog 4:e18. 2008
    The antiviral factor tripartite interaction motif 5alpha (Trim5alpha) restricts a broad range of retroviruses in a species-specific manner...
  74. doi Ubiquitination of E3 ubiquitin ligase TRIM5 alpha and its potential role
    Keiko Yamauchi
    Department of Cardiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    FEBS J 275:1540-55. 2008
    ..Here, we show that TRIM5alpha functions as a RING-finger-type E3 ubiquitin ligase both in vitro and in vivo and ubiquitinates itself in ..
  75. doi TRIM5alpha-independent anti-human immunodeficiency virus type 1 activity mediated by cyclophilin A in Old World monkey cells
    Emi E Nakayama
    Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Suita Shi, Osaka, Japan
    Virology 375:514-20. 2008
    ..b>TRIM5alpha is an antiretroviral factor influencing species-specific retroviral replication in Old World monkey (OWM) cells...
  76. pmc The structural biology of HIV assembly
    Barbie K Ganser-Pornillos
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 18:203-17. 2008
    ....
  77. pmc Proteasomal degradation of TRIM5alpha during retrovirus restriction
    Christopher James Rold
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America
    PLoS Pathog 4:e1000074. 2008
    The host protein TRIM5alpha inhibits retroviral infection at an early post-penetration stage by targeting the incoming viral capsid...
  78. doi Silencing of tripartite motif protein (TRIM) 5alpha mediated anti-HIV-1 activity by truncated mutant of TRIM5alpha
    Hikoichiro Maegawa
    Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    J Virol Methods 151:249-56. 2008
    ..It was found that both naturally occurring and artificial TRIM5alpha variants lacking the SPRY domain could silence TRIM5alpha activity...
  79. pmc A human TRIM5alpha B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virus
    Felipe Diaz-Griffero
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Department of Pathology, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 378:233-42. 2008
    Human TRIM5alpha restricts N-tropic murine leukemia virus (N-MLV) but not B-tropic MLV (B-MLV) infection. Here we study B30...
  80. pmc Restriction of retroviral replication by APOBEC3G/F and TRIM5alpha
    Hendrik Huthoff
    Department of Infectious Diseases, Guy s, King s and St Thomas School of Medicine, London SE1 9RT, UK
    Trends Microbiol 16:612-9. 2008
    ..Examples of such molecules are APOBEC3G, APOBEC3F and TRIM5alpha. APOBEC3G and APOBEC3F are cytidine deaminases that are able to strongly inhibit retroviral replication by at ..
  81. pmc An invariant surface patch on the TRIM5alpha PRYSPRY domain is required for retroviral restriction but dispensable for capsid binding
    Sarah Sebastian
    Department of Microbiology, Columbia University, New York, New York 10032, USA
    J Virol 83:3365-73. 2009
    b>TRIM5alpha is a retrovirus restriction factor in the host cell cytoplasm that blocks infection before provirus establishment. Restriction activity requires capsid (CA)-specific recognition by the PRYSPRY domain of TRIM5alpha...
  82. pmc Modulation of HIV-1 infectivity and cyclophilin A-dependence by Gag sequence and target cell type
    Saori Matsuoka
    INSERM U941, Paris 75010, France
    Retrovirology 6:21. 2009
    ..can markedly reduce viral fitness, and interactions of CA with host proteins such as cyclophilin A (CypA) and TRIM5alpha can have important effects on viral infectivity...
  83. pmc A B-box 2 surface patch important for TRIM5alpha self-association, capsid binding avidity, and retrovirus restriction
    Felipe Diaz-Griffero
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Virol 83:10737-51. 2009
    b>TRIM5alpha is a tripartite motif (TRIM) protein that consists of RING, B-box 2, coiled-coil, and B30.2(SPRY) domains...
  84. pmc Human immunodeficiency virus, restriction factors, and interferon
    Stuart Neil
    Department of Infectious Disease, King s College London School of Medicine, Guy s Hospital, London, United Kingdom
    J Interferon Cytokine Res 29:569-80. 2009
    ..Such knowledge could allow for novel forms of therapeutic intervention in pathogenic retroviral infections, as well as the development of animal models of human disease...
  85. pmc Rubella vaccine-induced cellular immunity: evidence of associations with polymorphisms in the Toll-like, vitamin A and D receptors, and innate immune response genes
    Inna G Ovsyannikova
    Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, Rochester, MN 55905, USA
    Hum Genet 127:207-21. 2010
    ..This is the first paper to present evidence that polymorphisms in the TLR, vitamin A, vitamin D receptor, and innate immunity genes can influence adaptive cytokine responses to rubella vaccination...
  86. doi Anti-retroviral activity of TRIM5 alpha
    Emi E Nakayama
    Department of Viral Infections, Research Institute for Microbial Disease, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Rev Med Virol 20:77-92. 2010
    ..Accurate structural analysis of the binding surface between the viral capsid protein and TRIM5 alpha SPRY is thus required for the development of new antiretroviral drugs that enhance anti-HIV-1 activity of human TRIM5 alpha...
  87. pmc p62/sequestosome-1 associates with and sustains the expression of retroviral restriction factor TRIM5alpha
    Christopher O'Connor
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA
    J Virol 84:5997-6006. 2010
    ..proteins mediate a potent block to the cross-species transmission of retroviruses, the most well known being the TRIM5alpha protein from rhesus macaques, which potently inhibits human immunodeficiency virus type 1 (HIV-1) infection...
  88. doi Inhibitory effect of human TRIM5alpha on HIV-1 production
    Xianfeng Zhang
    Institute for Genetic Medicine, Hokkaido University, Kita 15, Nishi 7, Kita ku, Sapporo 060 0815, Japan
    Microbes Infect 12:768-77. 2010
    Tripartite motif-containing 5 isoform-alpha (TRIM5alpha), a host restriction factor, blocks infection of some retroviruses at a post-entry, pre-integration stage in a species-specific manner. A recent report by Sakuma et al...
  89. pmc A TRIM5alpha exon 2 polymorphism is associated with protection from HIV-1 infection in the Pumwani sex worker cohort
    Heather Price
    Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, Manitoba, Canada
    AIDS 24:1813-21. 2010
    The innate immune component TRIM5alpha has the ability to restrict retrovirus infection in a species-specific manner. TRIM5alpha of some primate species restricts infection by HIV-1, whereas human TRIM5alpha lacks this specificity...
  90. pmc A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5 alpha
    Ayumu Kuroishi
    Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Retrovirology 7:58. 2010
    ..This virus (NL-4/5S6/7SvifS) was supposed to escape from host restriction factors cyclophilin A, CM TRIM5 alpha, and APOBEC3G. However, the replicative capability of NL-4/5S6/7SvifS in human cells was severely impaired...
  91. pmc Small-molecule inhibition of human immunodeficiency virus type 1 infection by virus capsid destabilization
    Jiong Shi
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    J Virol 85:542-9. 2011
    ..This study highlights uncoating as a step in the HIV-1 life cycle that is susceptible to small molecule intervention...
  92. pmc Human Trim5α has additional activities that are uncoupled from retroviral capsid recognition
    Semih U Tareen
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Virology 409:113-20. 2011
    ..Our results demonstrate functions of Trim5α that are not dependent on recognizing the retroviral capsid...
  93. pmc Revisiting HIV-1 uncoating
    Nathalie Arhel
    Department of Virology, URA3015, Institut Pasteur, 25 28 rue du Dr, Roux, 75015 Paris, France
    Retrovirology 7:96. 2010
    ....
  94. pmc Hexagonal assembly of a restricting TRIM5alpha protein
    Barbie K Ganser-Pornillos
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 108:534-9. 2011
    ..Capsid recognition therefore involves a synergistic combination of direct binding interactions, avidity effects, templated assembly, and lattice complementarity...
  95. pmc Recent insights into the mechanism and consequences of TRIM5α retroviral restriction
    Jaya Sastri
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153, USA
    AIDS Res Hum Retroviruses 27:231-8. 2011
    ..We also discuss how polymorphisms within human and rhesus macaque populations have been demonstrated to affect disease progression of immunodeficiency viruses in these species...
  96. pmc The antiviral spectra of TRIM5α orthologues and human TRIM family proteins against lentiviral production
    Seiga Ohmine
    Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America
    PLoS ONE 6:e16121. 2011
    ..Although all TRIM family proteins have RBCC domains, it remains elusive whether they possess similar late-restriction activities...
  97. pmc Rhesus TRIM5α disrupts the HIV-1 capsid at the inter-hexamer interfaces
    Gongpu Zhao
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 7:e1002009. 2011
    ....
  98. pmc Novel escape mutants suggest an extensive TRIM5α binding site spanning the entire outer surface of the murine leukemia virus capsid protein
    Sadayuki Ohkura
    Division of Virology, MRC National Institute for Medical Research, London, United Kingdom
    PLoS Pathog 7:e1002011. 2011
    ..We conclude that interactions involving much, if not all, of the surface of CA are vital for TRIM5α binding...
  99. pmc SUMO-interacting motifs of human TRIM5α are important for antiviral activity
    Gloria Arriagada
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, United States of America
    PLoS Pathog 7:e1002019. 2011
    ..We propose that at least a portion of the antiviral activity of TRIM5α is mediated through the binding of its SIMs to SUMO-conjugated CA...
  100. pmc TRIM5 is an innate immune sensor for the retrovirus capsid lattice
    Thomas Pertel
    Department of Microbiology and Molecular Medicine, University of Geneva, Geneva CH 1211, Switzerland
    Nature 472:361-5. 2011
    ....
  101. pmc Trafficking of some old world primate TRIM5α proteins through the nucleus
    Felipe Diaz-Griffero
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Retrovirology 8:38. 2011
    ..Interestingly, other proteins from the TRIM family localize to the nucleus. Therefore, we tested the possibility that TRIM5 proteins traffic to the nucleus and the impact of this trafficking on retroviral restriction...

Research Grants43

  1. Joseph G Sodroski; Fiscal Year: 2014
    ..The proposed work will attempt to understand how TRIM51 works and how its activity can be improved. ..
  2. Optimized Adaptation of Simian-tropic R5 HIV Clade C to Pig-tailed Macaques
    RUTH MARGRIT RUPRECHT; Fiscal Year: 2013
    ..abstract_text> ..
  3. The Role of CyclophilinA in the Evolution of Retroviral Restriction
    RAYMOND MALFAVON BORJA; Fiscal Year: 2010
    ..Earning a NRSA Fellowship will ensure that I am able to commit the next several years to the work that I have outlined, and through my work I will be contributing to the scientific community and general public. ..
  4. Felipe Diaz-Griffero; Fiscal Year: 2016
    ....
  5. Paul D Bieniasz; Fiscal Year: 2016
    ..In addition, understanding species-specific variation and engineering resistance to antiretroviral genes could facilitate the development of animal models of human retroviral infection. ..
  6. The role of UBE2V1 in HIV-1 restriction in primary monocytes
    Li Wu; Fiscal Year: 2013
    ..Ultimately, the knowledge learned from the proposed studies will enable design of novel approaches to block HIV-1 replication in target cells. ..
  7. Structural Basis of Retroviral Restriction by TRIM5alpha
    DMITRI N IVANOV; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): TRIM5alpha proteins bind retroviral capsids after cell entry and restrict retroviral infection by blocking reverse transcription and/or integration of the viral genetic material {Nisole, 2005 #305;..
  8. Regulators and mediators of TRIM5 function
    Jacek Skowronski; Fiscal Year: 2010
    ..Knowledge gained from our studies could lead to the conception of new strategies to control HIV infection and to the identification of new targets for development of anti-retroviral drugs. ..
  9. HIV-1 Resistance to Type I Interferon in Macaques
    Jason T Kimata; Fiscal Year: 2013
    ..If successful, these studies should provide valuable insight ito HIV-1-host interactions influencing transmission and disease. Ultimately, the studies should inform the development of a macaque-model of HIV-1 pathogenesis. ..
  10. Eric M Poeschla; Fiscal Year: 2016
    ..This kind of innate immunity is mediated by proteins such as APOBEC3 proteins, TRIM5alpha, TRIMCyp, and BST2/Tetherin. To infect new species, retroviruses must evolve specific countermeasures...
  11. Welkin E Johnson; Fiscal Year: 2015
    ..Together, work on these three aims will illuminate the biological relevance of TRIM5- mediated suppression and lead to improved animal models of AIDS. ..
  12. Host Factor Genetics and Susceptibility to Retroviral Infection
    Welkin Johnson; Fiscal Year: 2009
    ..abstract_text> ..
  13. CHRISTOPHER R AIKEN; Fiscal Year: 2016
    ..Aim 5. Identification of novel capsid-interacting host factors. These studies will answer fundamental question in retrovirus biology and will clarify the mechanisms of restriction by endogenous host cell factors. ..
  14. Sunil K Ahuja; Fiscal Year: 2014
    ..PUBLIC HEALTH RELEVANCE: The studies proposed will provide new insights into the host factors that influence susceptibility to HIV and AIDS, and these findings could provide new ways to develop therapies and vaccines. ..
  15. A SIV/Rhesus Monkey Penile Mucosal Transmission Model
    WENDY WEN LI YEH; Fiscal Year: 2011
    ..This animal mucosal transmission model has the potential to have a transformative impact on developing intervention strategies to block HIV-1 acquisition in the human male genital mucosa. ..
  16. Edward M Campbell; Fiscal Year: 2015
    DESCRIPTION (provided by applicant): This application will seek to better understand the mechanism by which the TRIM5alpha protein from rhesus macaques (rhTRIM5alpha) inhibits HIV-1 infection by defining the molecular and cellular ..
  17. Role of the B-box-2 Domain in HIV-1 Restriction by TRIM5alpha proteins
    Felipe Diaz Griffero; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): The recently discovered rhesus monkey protein TRIM5alpha (TRIM5alpha-rh) is responsible for the strong restriction imposed to human immunodeficiency virus (HIV-1) infection by these primates...
  18. Welkin E Johnson; Fiscal Year: 2016
    ..The TRIM5alpha protein functions as an intrinsic inhibitor of retroviral replication, and the primate TRIM5 locus displays an ..
  19. Determinants of host adaptation in a Macaca nemestrina-adapted HIV-2 isolate
    Gregory Brennan; Fiscal Year: 2009
    ....
  20. TRIM5alpha, HIV-1 Uncoating and the Ubiquitin-Proteasome Pathway
    LESA BLACK; Fiscal Year: 2007
    ..Studies have identified TRIM5alpha which blocks retroviral infection by targeting the viral capsid after entry, but before completion of reverse ..
  21. Analysis of restriction factor function in situ
    Edward M Campbell; Fiscal Year: 2010
    ....
  22. TRIM5alpha-mediated block of HIV-1 production
    Yasuhiro Ikeda; Fiscal Year: 2008
    ..Rhesus monkey TRIM5alpha (rhTRIM5a) is a potent intrinsic anti-retroviral factor which targets incoming HIV-1 capsid and promotes its ..
  23. HIV Infection of Non-Dividing Cells
    Michael Emerman; Fiscal Year: 2010
    ..The identification of the precise viral determinants that allow it access to non-dividing cells may present new drug targets against HIV. ..
  24. Expansion of HIV infected T cells for immunotherapy
    James Riley; Fiscal Year: 2005
    ..Successful completion of these aims will pave the way for second generation autologous transfusion trials in which the ability of selected subsets of expanded T cells to fight opportunistic and HIV infection(s) can be measured. ..
  25. Training of Claflin Minorities at the USC Cancer Center
    Omar Bagasra; Fiscal Year: 2005
    ..The PIs of these projects will be Dr. Omar Bagasra of Claflin University, and Dr. Kim E. Creek of the SCCC. ..
  26. Development of tumor based antigen presenting cells
    James Riley; Fiscal Year: 2008
    ..Furthermore, these studies will provide the foundation for conducting a clinical trial. ..
  27. Actions of Vif and APOBEC3 proteins in HIV-1 Replication
    Yong Hui Zheng; Fiscal Year: 2010
    ..Such findings may provide a more effective modality for the treatment of HIV infection to be used in conjunction with other chemotherapy and vaccines. ..
  28. Mechanisms of AID-dependent Adaptive and Innate Immunity
    Reuben S Harris; Fiscal Year: 2010
    ..abstract_text> ..
  29. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  30. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  31. Accessory roles of Tat in HIV-1 replication
    Michael Emerman; Fiscal Year: 2005
    ..Thus, the overall goal of this proposal is to identify functions attributable to the second exon of Tat and to ascertain the role of these Tat functions in HIV-1 replication and pathogenesis. ..
  32. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  33. DELETION MUTAGENESIS WITHIN NONCODING SEGMENTS OF HIV1
    Mark Wainberg; Fiscal Year: 2001
    ..6. To determine whether these attenuated SIV constructs may protect against subsequent challenge with virulent forms of SIV and SIV/HIV hybrids. ..
  34. Lentiviral Redirected T Cells for B Cancer Immunotherapy
    James Riley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  35. Structure of the Retroviral Restriction Factor, TRIM5alpha
    Joseph Sodroski; Fiscal Year: 2008
    ..Human immunodeficiency virus (HIV) cannot infect monkeys because these animals make a resistance factor called TRIM5alpha. Human TRIM5alpha only modestly blocks HIV...
  36. Retrovirus Restriction by Trima5alpha
    Paul Bieniasz; Fiscal Year: 2009
    ..The recent identification of TRIM5alpha as one of this class of retrovirus resistance factors makes this an appropriate time to expand our efforts to ..
  37. Regulation of human T cell activation by the CD28 family
    James Riley; Fiscal Year: 2008
    ..The data generated from these studies will better define the relationship between antigen dependent and independent signals and enable new strategies to modulate T cell activation for therapeutic applications. ..
  38. Overcoming blocks to HIV-1 replication in rhesus macaques.
    Theodora Hatziioannou; Fiscal Year: 2007
    ..If successful, this proposal will lead to improved animal models for HIV-1 infection and will considerably facilitate the development and testing of drug and vaccine interventions. [unreadable] [unreadable] [unreadable]..
  39. Transgenic mouse for modifying antiviral mAb specificity
    Welkin Johnson; Fiscal Year: 2006
    ..In the second case, the hypothesis to be tested is that native envelope complexes contain conformationally induced epitopes that are not present in the monomeric envelope protein. ..
  40. Replication and Persistence of Primate Lentiviruses
    Welkin Johnson; Fiscal Year: 2009
    ....
  41. Identification of Trim5alpha cofactors
    NATHANIEL LANDAU; Fiscal Year: 2008
    ..This understanding can be useful for the development of new drugs for AIDS. [unreadable] [unreadable] [unreadable]..