TOP2A

Summary

Gene Symbol: TOP2A
Description: topoisomerase (DNA) II alpha
Alias: TOP2, TP2A, DNA topoisomerase 2-alpha, DNA gyrase, DNA topoisomerase (ATP-hydrolyzing), DNA topoisomerase II, 170 kD, DNA topoisomerase II, alpha isozyme, topoisomerase (DNA) II alpha 170kDa
Species: human

Top Publications

  1. ncbi TOP2A and HER2 gene amplification as predictors of response to anthracycline treatment in breast cancer
    Kenneth Villman
    Department of Oncology, Orebro University Hospital, Sweden
    Acta Oncol 45:590-6. 2006
  2. pmc Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs DNA damage induced by topoisomerases I and II and base alkylation in vertebrate cells
    Junko Murai
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Biol Chem 287:12848-57. 2012
  3. ncbi HER-2/neu and topoisomerase IIa gene amplification and protein expression in invasive breast carcinomas: chromogenic in situ hybridization and immunohistochemical analyses
    Rohit Bhargava
    Department of Pathology, Memorial Sloan Kettering, Cancer Center, New York, NY 10021, USA
    Am J Clin Pathol 123:889-95. 2005
  4. doi Aberrations of ERBB2 and TOP2A genes in breast cancer
    Kirsten Vang Nielsen
    Dako A S, Glostrup, Denmark
    Mol Oncol 4:161-8. 2010
  5. pmc Topoisomerase II alpha and responsiveness of breast cancer to adjuvant chemotherapy
    F P O'Malley
    Sunnybrook Odette Cancer Centre, University of Toronto, 2075 Bayview Ave, Toronto, Ontario, Canada
    J Natl Cancer Inst 101:644-50. 2009
  6. doi Association between HER2, TOP2A, and response to anthracycline-based preoperative chemotherapy in high-risk primary breast cancer
    Gottfried E Konecny
    UCLA Translational Oncology Research Lab, David Geffen School of Medicine, University of California Los Angeles, 2825 Santa Monica Blvd, Suite 200, Santa Monica, CA, 90404 2429, USA
    Breast Cancer Res Treat 120:481-9. 2010
  7. ncbi Human topoisomerase IIalpha and IIbeta interact with the C-terminal region of p53
    I G Cowell
    School of Biochemistry and Genetics, University of Newcastle, Newcastle upon Tyne, NE2 4HH, United Kingdom
    Exp Cell Res 255:86-94. 2000
  8. ncbi A biochemical analysis of topoisomerase II alpha and beta kinase activity found in HIV-1 infected cells and virus
    Anand K Kondapi
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India
    Arch Biochem Biophys 441:41-55. 2005
  9. doi Topoisomerase IIalpha mRNA and protein expression in ovarian carcinoma: correlation with clinicopathological factors and prognosis
    Areeg Faggad
    Institute of Pathology, Charite University Hospital, Berlin, Germany
    Mod Pathol 22:579-88. 2009
  10. pmc Topoisomerase II alpha status in renal medullary carcinoma: immuno-expression and gene copy alterations of a potential target of therapy
    Roula Albadine
    Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA
    J Urol 182:735-40. 2009

Research Grants

  1. FUNCTIONAL AND MECHANISTIC STUDIES OF DNA TOPOISOMERASES
    Leroy Liu; Fiscal Year: 2001
  2. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
  3. Matthew J O'Connell; Fiscal Year: 2015
  4. Mechanism of action of TOP2-directed anticancer drugs
    Leroy F Liu; Fiscal Year: 2012
  5. John L Nitiss; Fiscal Year: 2014
  6. Chemical Glycobiology on Anthracyclines
    Peng Wang; Fiscal Year: 2009
  7. Diane J Mathis; Fiscal Year: 2015
  8. DNA TOPOISOMERASE II - DOMAINS, BINDING SITES & FUNCTION
    STEPHEN WORLAND; Fiscal Year: 1993
  9. Howard D Strickler; Fiscal Year: 2016
  10. Topoisomerase-Associated Genome Instability
    SABRINA COTE; Fiscal Year: 2013

Detail Information

Publications219 found, 100 shown here

  1. ncbi TOP2A and HER2 gene amplification as predictors of response to anthracycline treatment in breast cancer
    Kenneth Villman
    Department of Oncology, Orebro University Hospital, Sweden
    Acta Oncol 45:590-6. 2006
    The purpose of this study was to evaluate amplification of topoisomerase IIalpha (TOP2A) and HER2 genes as predictors of response to chemotherapy in advanced breast cancer...
  2. pmc Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs DNA damage induced by topoisomerases I and II and base alkylation in vertebrate cells
    Junko Murai
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Biol Chem 287:12848-57. 2012
    ..Together, our findings reveal a broad involvement of Tdp1 in DNA repair and clarify the role of human TDP1 in the repair of Top2-induced DNA damage.
  3. ncbi HER-2/neu and topoisomerase IIa gene amplification and protein expression in invasive breast carcinomas: chromogenic in situ hybridization and immunohistochemical analyses
    Rohit Bhargava
    Department of Pathology, Memorial Sloan Kettering, Cancer Center, New York, NY 10021, USA
    Am J Clin Pathol 123:889-95. 2005
    ..Identification of topo2a and HER-2 status might have therapeutic and prognostic implications...
  4. doi Aberrations of ERBB2 and TOP2A genes in breast cancer
    Kirsten Vang Nielsen
    Dako A S, Glostrup, Denmark
    Mol Oncol 4:161-8. 2010
    Copy number changes in TOP2A have frequently been linked to ERBB2 (HER2) amplified breast cancers...
  5. pmc Topoisomerase II alpha and responsiveness of breast cancer to adjuvant chemotherapy
    F P O'Malley
    Sunnybrook Odette Cancer Centre, University of Toronto, 2075 Bayview Ave, Toronto, Ontario, Canada
    J Natl Cancer Inst 101:644-50. 2009
    Amplification or deletion of the topoisomerase II alpha (TOP2A) gene in breast cancers has been postulated to be more closely associated with responsiveness to anthracycline-containing chemotherapy than amplification of the human ..
  6. doi Association between HER2, TOP2A, and response to anthracycline-based preoperative chemotherapy in high-risk primary breast cancer
    Gottfried E Konecny
    UCLA Translational Oncology Research Lab, David Geffen School of Medicine, University of California Los Angeles, 2825 Santa Monica Blvd, Suite 200, Santa Monica, CA, 90404 2429, USA
    Breast Cancer Res Treat 120:481-9. 2010
    ..response to anthracycline-based chemotherapy may be more likely related to the concomitant amplification of the TOP2A gene...
  7. ncbi Human topoisomerase IIalpha and IIbeta interact with the C-terminal region of p53
    I G Cowell
    School of Biochemistry and Genetics, University of Newcastle, Newcastle upon Tyne, NE2 4HH, United Kingdom
    Exp Cell Res 255:86-94. 2000
    ..Furthermore, we show that the regulatory C-terminal basic region of p53 (residues 364-393) is necessary and sufficient for interaction with DNA topo II...
  8. ncbi A biochemical analysis of topoisomerase II alpha and beta kinase activity found in HIV-1 infected cells and virus
    Anand K Kondapi
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India
    Arch Biochem Biophys 441:41-55. 2005
    ..The catalytic inhibitions by serine kinase inhibitors further suggest that the alpha and beta kinase activities associated with virus are distinctly different...
  9. doi Topoisomerase IIalpha mRNA and protein expression in ovarian carcinoma: correlation with clinicopathological factors and prognosis
    Areeg Faggad
    Institute of Pathology, Charite University Hospital, Berlin, Germany
    Mod Pathol 22:579-88. 2009
    ..Our results encourage the further evaluation of the prognostic and predictive values of Top IIalpha expression in ovarian carcinoma, which might help to assess the patients' risk profile, and the planning of an individualized therapy...
  10. pmc Topoisomerase II alpha status in renal medullary carcinoma: immuno-expression and gene copy alterations of a potential target of therapy
    Roula Albadine
    Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA
    J Urol 182:735-40. 2009
    ....
  11. ncbi Immunohistochemical expression of topoisomerase II alpha and Her-2/neu in prostatic carcinoma and benign prostatic hyperplasia
    Eiman Adel Hasby
    The Department of Pathology, Faculty of Medicine, Tanta University
    J Egypt Natl Canc Inst 20:158-67. 2008
    ....
  12. doi Prognostication of soft tissue sarcomas based on chromosome 17q gene and protein status: evaluation of TOP2A, HER-2/neu, and survivin
    Isabela Werneck da Cunha
    Hospital do Cancer A C Camargo, Fundacao Antonio Prudente, Sao Paulo, Brazil
    Ann Surg Oncol 19:1790-9. 2012
    Topoisomerase 2 alpha (TOP2A), HER-2/neu, and survivin are genes that lie on chromosome 17 and correlate with the prognosis and prediction of target-driven therapy against tumors...
  13. ncbi Identification of promoter elements responsible for transcriptional inhibition of polo-like kinase 1 and topoisomerase IIalpha genes by p21(WAF1/CIP1/SDI1)
    Hongming Zhu
    Department of Molecular Genetics, University of Illinois at Chicago, 60607 7170, USA
    Cell Cycle 1:59-66. 2002
    ..These results indicate that inhibition of cell division-associated genes by p21 is mediated by different but overlapping mechanisms, which are not a general con-sequence of cell cycle arrest...
  14. ncbi retrospective analysis of topoisomerase IIa amplifications and deletions as predictive markers in primary breast cancer patients randomly assigned to cyclophosphamide, methotrexate, and fluorouracil or cyclophosphamide, epirubicin, and fluorouracil: Danis
    Ann S Knoop
    Oncological Department, Odense University Hospital DK 5000, Odense C, Denmark
    J Clin Oncol 23:7483-90. 2005
    The aim of the study was to evaluate the predictive value of HER2 and topoisomerase IIalpha gene (TOP2A) for the efficacy of epirubicin in the adjuvant setting of breast cancer patients.
  15. ncbi Topoisomerase IIalpha gene expression is regulated by the p53 tumor suppressor gene in nonsmall cell lung carcinoma patients
    DaGe Liu
    Second Department of Surgery, Kagawa Medical University, Kagawa, Japan
    Cancer 94:2239-47. 2002
    ..Previous experimental studies using cell lines reported that Topo IIalpha expression was negatively regulated by wild-type p53 through the gene's promoter region...
  16. ncbi Topoisomerase IIalpha gene (TOP2A) amplification and deletion in cancer--more common than anticipated
    T A H Järvinen
    The Burnham Institute, La Jolla, CA 92037, USA
    Cytopathology 14:309-13. 2003
    ..other biologically relevant genes with altered copy numbers, among these genes is the topoisomerase IIalpha (TOP2A)...
  17. ncbi A study of the topoisomerase II activity in HIV-1 replication using the ferrocene derivatives as probes
    Anand K Kondapi
    Department of Biochemistry, University of Hyderabad, School of Life Sciences, Hyderabad 500 046, India
    Arch Biochem Biophys 450:123-32. 2006
    ..Further analysis, using polymerase chain reaction and western blot, showed that both the Topoisomerase II alpha and beta isoforms are present in the pre-integration complexes, suggesting their significant role in HIV-1 replication...
  18. ncbi Does polysomy of chromosome 17 have a role in ERBB2 and topoisomerase IIalpha expression? Gene, mRNA and protein expression: a comprehensive analysis
    Cristina Corzo
    Servei d Oncologia Medica, Hospital del Mar, IMAS, Barcelona, Spain
    Tumour Biol 28:221-8. 2007
    ..The aim of the present study was to clarify the implication of polysomy 17 in ERBB2 and topoisomerase IIalpha expression. In addition, we assessed the relation of ERBB2 and topoisomerase IIalpha gene dosage to mRNA and protein levels...
  19. pmc HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha
    Michal Stros
    Laboratory of Analysis of Chromosomal Proteins, Academy of Sciences of the Czech Republic, Institute of Biophysics, Brno, Czech Republic
    Nucleic Acids Res 37:2070-86. 2009
    ....
  20. pmc TIF1beta/KAP-1 is a coactivator of the orphan nuclear receptor NGFI-B/Nur77
    Juliette Rambaud
    Laboratory of Molecular Genetics, Institut de Recherches Cliniques de Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 284:14147-56. 2009
    ..However, the actions of TIF1beta and SRC2 were mapped to different NGFI-B AF-1 subdomains. Taken together, these results indicate that TIF1beta is an important coactivator of NGFI-B-dependent transcription...
  21. pmc Telomerase-associated protein 1, HSP90, and topoisomerase IIalpha associate directly with the BLM helicase in immortalized cells using ALT and modulate its helicase activity using telomeric DNA substrates
    Saumitri Bhattacharyya
    Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University College of Medicine, Columbus, Ohio 43210 2207, USA
    J Biol Chem 284:14966-77. 2009
    ..Initial studies suggest that knockdown of BLM in ALT cells reduces average telomere length but does not do so in cells using telomerase...
  22. doi The ability of biomarkers to predict systemic progression in men with high-risk prostate cancer treated surgically is dependent on ERG status
    R Jeffrey Karnes
    Department of Urology, Laboratory Medicine and Pathology, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    Cancer Res 70:8994-9002. 2010
    The objective of this study was to assess the relationship of the tumor protein levels of TOP2A and MIB-1 and ERG status with cancer-specific outcomes in men with high-risk prostate cancer treated by radical prostatectomy (RP)...
  23. pmc Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22
    M Tsai-Pflugfelder
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
    Proc Natl Acad Sci U S A 85:7177-81. 1988
    ..II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes...
  24. pmc Structure and function of type II DNA topoisomerases
    P M Watt
    Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, U K
    Biochem J 303:681-95. 1994
  25. pmc Functional interaction between human topoisomerase IIalpha and retinoblastoma protein
    U G Bhat
    Division of Molecular Pharmacology, Department of Molecular Genetics M C 669, College of Medicine, University of Illinois, Chicago, IL 60607 7173, USA
    Proc Natl Acad Sci U S A 96:7859-64. 1999
    ..We conclude that topo IIalpha associates physically with Rb in interactions that appear to have functional significance...
  26. ncbi Using a biochemical approach to identify the primary dimerization regions in human DNA topoisomerase IIalpha
    L Bjergbaek
    Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, 8000 Arhus C, Denmark
    J Biol Chem 274:26529-36. 1999
    ..The result indicates that secondary interactions occur by ATP analog-mediated clamp closing when the subunits are brought together on DNA...
  27. ncbi Modulation of human DNA topoisomerase IIalpha function by interaction with 14-3-3epsilon
    E U Kurz
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Health Sciences Center and University of Colorado Cancer Center, Denver, Colorado 80262, USA
    J Biol Chem 275:13948-54. 2000
    ..The association of topo II with 14-3-3 proteins does not extend to all 14-3-3 isoforms. No protein interaction or disruption of topo II function was observed with 14-3-3final sigma...
  28. ncbi SUMO-1 conjugation to human DNA topoisomerase II isozymes
    Y Mao
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 275:26066-73. 2000
    ..These studies raise the question on whether SUMO-1 conjugation to topoisomerases is an indirect result of a DNA damage response or a direct result because of protein conformational changes...
  29. ncbi DNA topoisomerase IIalpha interacts with CAD nuclease and is involved in chromatin condensation during apoptotic execution
    F Durrieu
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Curr Biol 10:923-6. 2000
    ..In addition, we show that CAD binds to Topo IIalpha, and that their association enhances the decatenation activity of Topo IIalpha in vitro...
  30. ncbi The SUMO pathway is required for selective degradation of DNA topoisomerase IIbeta induced by a catalytic inhibitor ICRF-193(1)
    Sevim Isik
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    FEBS Lett 546:374-8. 2003
    ..When the SUMO conjugating enzyme Ubc9 was conditionally knocked out, the ICRF-induced degradation of topoisomerase IIbeta did not occur, suggesting that the SUMO modification pathway is essential for the degradation...
  31. ncbi HER-2 and TOP2A coamplification in urinary bladder cancer
    Ronald Simon
    Institute for Pathology and Urologic Clinics, University of Basel, Basel, Switzerland
    Int J Cancer 107:764-72. 2003
    ..In breast cancer, the adjacent TOP2A gene, the molecular target for several anticancer drugs, is frequently coamplified together with HER-2...
  32. ncbi Identification of toposome, a novel multisubunit complex containing topoisomerase IIalpha
    Chee Gun Lee
    Department of Biochemistry and Molecular Biology, Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark 07013, USA
    Cell Cycle 3:638-47. 2004
    ..These results suggest that toposome is a dynamic complex whose assembly or activation is subject to cell cycle regulation...
  33. ncbi 1,4-Benzoquinone is a topoisomerase II poison
    R Hunter Lindsey
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 43:7563-74. 2004
    ..The present findings are consistent with the hypothesis that topoisomerase IIalpha plays a role in the initiation of specific leukemias induced by benzene and its metabolites...
  34. pmc Impact of the C-terminal domain of topoisomerase IIalpha on the DNA cleavage activity of the human enzyme
    Jennifer S Dickey
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 44:11546-54. 2005
    ..Therefore, the C-terminal domain of human topoisomerase IIalpha appears to play significant roles in modulating the DNA cleavage/ligation reaction of the enzyme and its response to anticancer agents...
  35. pmc C-terminal regions of topoisomerase IIalpha and IIbeta determine isoform-specific functioning of the enzymes in vivo
    Rene M Linka
    Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich Heine University, Medical School, Moorenstrasse 5, D 40225 Dusseldorf, Germany
    Nucleic Acids Res 35:3810-22. 2007
    ..Our results show that it is the CTRs of human topoisomerase II that determine their isoform-specific functions in proliferating cells. They also indicate persistence of some functional redundancy between the two isoforms...
  36. doi Upregulation of topoisomerase IIalpha expression in advanced gallbladder carcinoma: a potential chemotherapeutic target
    Mitsutsune Washiro
    Department of General Surgery, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuoh ku, Chiba, 260 8670, Japan
    J Cancer Res Clin Oncol 134:793-801. 2008
    ..The aim of this study was to identify cancer-specific cellular targets that would form the basis for some therapeutic approaches for this disease...
  37. doi Genomic analysis of the HER2/TOP2A amplicon in breast cancer and breast cancer cell lines
    Edurne Arriola
    The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, UK
    Lab Invest 88:491-503. 2008
    HER2 and TOP2A are targets for the therapeutic agents trastuzumab and anthracyclines and are frequently amplified in breast cancers...
  38. doi The value of TOP2A gene copy number variation as a biomarker in breast cancer: Update of DBCG trial 89D
    Kirsten Vang Nielsen
    Dako A S, Glostrup, Denmark
    Acta Oncol 47:725-34. 2008
    Previous analyses of TOP2A and HER2 in the Danish Breast Cancer Coopererative Group (DBCG) trial 89D suggested that TOP2A amplifications and possible also deletions are predictive markers for the effect of adjuvant epirubicin in patients ..
  39. doi Excision repair cross complementing-1 and topoisomerase IIalpha gene expression in small-cell lung cancer patients treated with platinum and etoposide: a retrospective study
    Paolo Ceppi
    Thoracic Oncology Unit, University of Torino, S Luigi Hospital, Orbassano Torino, Italy
    J Thorac Oncol 3:583-9. 2008
    ..Aim of the study was to quantify ERCC1, RRM1, and TopoIIalpha mRNA expression profile as predictive factors for response and survival in SCLC patients treated with platinum/etoposide...
  40. pmc Expression analysis of Barrett's esophagus-associated high-grade dysplasia in laser capture microdissected archival tissue
    Edmond Sabo
    Department of Pathology and Medicine, Rhode Island Hospital, Providence, Rhode Island 02903, USA
    Clin Cancer Res 14:6440-8. 2008
    ..The aim of this study was to determine the differential transcriptome of HGD compared with NDBE through gene microarray analysis of epithelial cells microdissected from archival tissue specimens...
  41. pmc Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes
    T D Chung
    Research and Development, Smith Kline and French Laboratories, King of Prussia, PA 19406
    Proc Natl Acad Sci U S A 86:9431-5. 1989
    ..These data provide genetic and immunochemical evidence for two Topo II isozymes...
  42. ncbi Cell cycle phase-specific phosphorylation of human topoisomerase II alpha. Evidence of a role for protein kinase C
    N J Wells
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom
    J Biol Chem 270:28357-63. 1995
    ..Our results implicate protein kinase C in the cell cycle phase-dependent modulation of topoisomerase II alpha enzymatic activity in human cells...
  43. ncbi Human topoisomerase II alpha is phosphorylated in a cell-cycle phase-dependent manner by a proline-directed kinase
    N J Wells
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, England
    Eur J Biochem 231:491-7. 1995
    ..Our data implicate one or more proline-directed kinases in the cell-cycle-dependent regulation of topoisomerase II alpha enzyme activity in human cells...
  44. ncbi Serine 1524 is a major site of phosphorylation on human topoisomerase II alpha protein in vivo and is a substrate for casein kinase II in vitro
    N J Wells
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom
    J Biol Chem 269:29746-51. 1994
    ..Site-directed mutagenesis studies identified these casein kinase II-specific phosphorylation sites as serine 1524 and serine 1376...
  45. ncbi Modification of DNA topoisomerase II activity via direct interactions with the cyclic adenosine-3',5'-monophosphate response element-binding protein and related transcription factors
    D J Kroll
    Division of Medical Oncology, University of Colorado Health Sciences Center, Denver 80262
    Mol Endocrinol 7:305-18. 1993
    ....
  46. pmc Active heterodimers are formed from human DNA topoisomerase II alpha and II beta isoforms
    H Biersack
    Department of Molecular and Structural Biology, University of Aarhus, Denmark
    Proc Natl Acad Sci U S A 93:8288-93. 1996
    ..The natural existence of an active heterodimeric subclass of topoisomerase II merits attention whenever topoisomerases II alpha and II beta function, localization, and cell cycle regulation are investigated...
  47. pmc Cell cycle-coupled relocation of types I and II topoisomerases and modulation of catalytic enzyme activities
    K N Meyer
    Medizinische Poliklinik, University of Wurzburg, Germany
    J Cell Biol 136:775-88. 1997
    ..Scaffold-associated topoisomerase IIalpha appears not to be involved in catalytic DNA turnover, though it may play a role in the replicational cycle of centriols, where it accumulates during M phase...
  48. ncbi Structural organization of the human TOP2A and TOP2B genes
    A J Lang
    Department of Pharmacology and Toxicology, Queen s University, Kingston, Ont K7L 3N6, Canada
    Gene 221:255-66. 1998
    ..Human topoisomerase IIalpha (TOP2A) is encoded by the TOP2A gene on chromosome 17q21-22, and human topoisomerase IIbeta (TOP2B) is encoded by the ..
  49. pmc Extracellular signal-regulated kinase activates topoisomerase IIalpha through a mechanism independent of phosphorylation
    P S Shapiro
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    Mol Cell Biol 19:3551-60. 1999
    ....
  50. ncbi Mitotic phosphorylation of DNA topoisomerase II alpha by protein kinase CK2 creates the MPM-2 phosphoepitope on Ser-1469
    A E Escargueil
    Laboratoire de Biologie et Pharmacologie des Tumeurs, CNRS UMR 8532, Institut Gustave Roussy PR2, Villejuif 94805 cedex, France
    J Biol Chem 275:34710-8. 2000
    ..In conclusion, we have identified protein kinase CK2 as a new MPM-2 kinase able to phosphorylate an important mitotic protein, topoisomerase II alpha, on Ser-1469...
  51. ncbi Deacetylase activity associates with topoisomerase II and is necessary for etoposide-induced apoptosis
    C A Johnson
    Chromatin and Gene Expression Group, Department of Anatomy, University of Birmingham Medical School, Birmingham B15 2TT, United Kingdom
    J Biol Chem 276:4539-42. 2001
    ..We suggest that chromatin remodeling by an HDAC-containing complex facilitates both topo II-catalyzed DNA rearrangement and etoposide-induced DNA damage in vivo...
  52. ncbi Markers of drug resistance in relapsing colon cancer
    A C Lazaris
    Department of Pathology, School of Medicine, The National and Capodistrian University of Athens, 75 Mikras Asias Street, Goudi, 115 27 Athens, Greece
    J Cancer Res Clin Oncol 128:114-8. 2002
    ..e., P-glycoprotein, MRP, and LRP) and of topoisomerase IIalpha in a series of 20 primary colon carcinomas and their recurrences...
  53. pmc Dynamics of human DNA topoisomerases IIalpha and IIbeta in living cells
    Morten O Christensen
    Department of Clinical Chemistry, Medizinische Poliklinik, University of Wurzburg, D 97070 Wurzburg, Germany
    J Cell Biol 157:31-44. 2002
    ..These observations suggest that topo II is not an immobile, structural component of the chromosomal scaffold or the interphase karyoskeleton, but rather a dynamic interaction partner of such structures...
  54. ncbi Transcriptional regulation of the human topoisomerase IIalpha gene
    Christian Bronner
    Institut National de la Santé et de la Recherche Médicale U425, Faculte de Pharmacie, Illkirch, France
    Anticancer Res 22:605-12. 2002
    ..Here, we also speculate on the role of a novelprotein (named ICBP90) that has been identified by the one-hybrid system assay as a protein able to bind to these CCAAT sequences...
  55. ncbi Phosphorylation of serine 1106 in the catalytic domain of topoisomerase II alpha regulates enzymatic activity and drug sensitivity
    Kenichi Chikamori
    Experimental Therapeutics Program, Taussig Cancer Center, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 278:12696-702. 2003
    ..These results demonstrate that Ca(2+)-regulated phosphorylation of Ser-1106 in the catalytic domain of topo II alpha modulates the enzymatic activity of this protein and sensitivity to topo II-targeting drugs...
  56. ncbi Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta
    Shelagh E L Mirski
    Cancer Research Laboratories, Queen s University, Ont, Kingston, Canada K7L 3N6
    Biochem Biophys Res Commun 306:905-11. 2003
    ..Our results provide the first evidence of active nuclear export of topoisomerase II and suggest it is mediated by a CRM1-dependent pathway...
  57. ncbi Kinetic analysis of human topoisomerase IIalpha and beta DNA binding by surface plasmon resonance
    Chrysoula Leontiou
    School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    FEBS Lett 554:206-10. 2003
    ..4 and 2.9 nM. We therefore conclude that neither isoform has any preference for a specific DNA substrate under the conditions used in these experiments...
  58. pmc Human topoisomerase IIalpha: targeting to subchromosomal sites of activity during interphase and mitosis
    Marta Agostinho
    Institute of Histology, Faculty of Medicine, University of Lisbon, 1649 028 Lisbon, Portugal
    Mol Biol Cell 15:2388-400. 2004
    ..Together, our data implicate several previously unsuspected activities in the partitioning of the enzyme between sites of activity and putative depots...
  59. ncbi Unique cell membrane expression of topoisomerase-II alpha as a useful diagnostic marker of liposarcoma
    Hideko Endo
    Department of Pathology, University of Tokushima School of Medicine, Tokushima, Japan
    Pathol Int 54:145-50. 2004
    ..Immunostaining using the antibody for Topo-II alpha seems to be useful in recognizing lipoblasts that have been overlooked in hematoxylin-eosin-stained preparations, and is a useful marker for diagnosing liposarcoma...
  60. ncbi Coamplified and overexpressed genes at ERBB2 locus in gastric cancer
    Asta Varis
    Departments of Pathology and Medical Genetics, Haartman Institute, University of Helsinki and Helsinki University Central Hospital Laboratory Diagnostics, Helsinki, Finland
    Int J Cancer 109:548-53. 2004
    ..for the amplicon formation because this region harbors the ERBB2 oncogene and several other targets, such as TOP2A and DARPP32...
  61. ncbi Functional compatibility between isoform alpha and beta of type II DNA topoisomerase
    Ayako Sakaguchi
    Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University Graduate School and Faculty of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Cell Sci 117:1047-54. 2004
    ..Double-knockdown experiments also revealed that topo II was required for shortening of the chromosome axis...
  62. ncbi Enhanced processing of UVA-irradiated DNA by human topoisomerase II in living cells
    Christian Mielke
    Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich Heine University, Medical School, Moorenstrasse 5, D 40225 Dusseldorf, Germany
    J Biol Chem 279:20559-62. 2004
    ....
  63. ncbi Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5
    Jisoo Yun
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1, Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 279:31296-303. 2004
    ..These findings demonstrate that GRDD can act as a stress-activated degron for regulating topo IIalpha stability, possibly through interaction with the MPN domain of Jab1/CSN5...
  64. pmc Construction, characterization, and complementation of a conditional-lethal DNA topoisomerase IIalpha mutant human cell line
    Adam J Carpenter
    Gene Targeting Group, Medical Research Council Clinical Sciences Centre, Imperial College Faculty of Medicine, Hammersmith Hospital Campus, London W12 ONN, United Kingdom
    Mol Biol Cell 15:5700-11. 2004
    ....
  65. ncbi BRCA1 participates in DNA decatenation
    Zhenkun Lou
    Department of Oncology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    Nat Struct Mol Biol 12:589-93. 2005
    ..Together these results suggest an important role of BRCA1 in DNA decatenation and reveal a previously unknown function of BRCA1 in the maintenance of genomic stability...
  66. pmc Altered expression pattern of topoisomerase IIalpha in ovarian tumor epithelial and stromal cells after platinum-based chemotherapy
    Radoslav Chekerov
    Department of Obstetrics and Gynecology, Charite University Hospital, Campus Virchow, Berlin, Germany
    Neoplasia 8:38-45. 2006
    The aim of this study was to evaluate the expression of topoisomerase IIalpha (TOP2A) in epithelial and stromal cells of ovarian cancer.
  67. pmc HMGB1 interacts with human topoisomerase IIalpha and stimulates its catalytic activity
    Michal Stros
    Laboratory of Analysis of Chromosomal Proteins, Academy of Sciences of the Czech Republic, Institute of Biophysics, Brno, Czech Republic
    Nucleic Acids Res 35:5001-13. 2007
    ..The observed abilities of HMGB1 to interact with topo IIalpha and promote topo IIalpha binding to DNA suggest a mechanism by which HMGB1 stimulates the catalytic activity of the enzyme via enhancement of DNA cleavage...
  68. ncbi Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads
    Jennifer M Spence
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    J Cell Sci 120:3952-64. 2007
    ....
  69. ncbi BRCA1-mediated ubiquitination inhibits topoisomerase II alpha activity in response to oxidative stress
    Hirokuni Shinagawa
    Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
    Antioxid Redox Signal 10:939-49. 2008
    ..Furthermore, the retinoblastoma protein (pRb) was required for the ubiquitination of topoisomerase IIalpha by BRCA1. We conclude that the functions of topoisomerase IIalpha are regulated by ubiquitination on exposure to oxidative stress...
  70. pmc Resolution of sister centromeres requires RanBP2-mediated SUMOylation of topoisomerase IIalpha
    Meelad M Dawlaty
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Cell 133:103-15. 2008
    ..Furthermore, mice with low amounts of RanBP2 are highly sensitive to tumor formation. Together, these data identify RanBP2 as a chromosomal instability gene that regulates Topo IIalpha by sumoylation and suppresses tumorigenesis...
  71. pmc Human topoisomerase II-alpha is highly expressed in sinonasal-inverted papilloma, but not in inflammatory polyp
    Tuvia Hadar
    Department of Head and Neck Surgery, Rabin Medical Center, Petah Tikva, Israel
    J Cell Mol Med 12:1551-8. 2008
    ....
  72. pmc NK314, a topoisomerase II inhibitor that specifically targets the alpha isoform
    Eriko Toyoda
    International Graduate School of Arts and Sciences, Yokohama City University, 22 2 Seto, Kanazawa Ku, Yokohama, Japan
    J Biol Chem 283:23711-20. 2008
    Topoisomerase II (Top2) is a ubiquitous nuclear enzyme that relieves torsional stress in chromosomal DNA during various cellular processes...
  73. pmc Topoisomerase II alpha gene copy loss has adverse prognostic significance in ERBB2-amplified breast cancer: a retrospective study of paraffin-embedded tumor specimens and medical charts
    Lydia Usha
    Division of Hematology and Oncology, Department of Medicine, Rush University, Chicago, Illinois, 60612, USA
    J Hematol Oncol 1:12. 2008
    ..In approximately 25% of these patients, the amplicon extends to the gene for topoisomerase II alpha (TOP2A), a target for anthracyclines...
  74. doi Expression of human DNA Topoisomerase II-alpha in squamous cell carcinoma of the larynx and its correlation with clinicopathologic variables
    Jacob Shvero
    Department of Otolaryngology, Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel
    Am J Clin Pathol 130:934-9. 2008
    ..008) and tumor grade (P = .034) independently predicted disease-free survival. These findings suggest that high Topo II-alpha expression may be a useful indicator of tumor aggressiveness and poor outcome in laryngeal SCC...
  75. pmc Topoisomerase IIalpha controls the decatenation checkpoint
    Kuntian Luo
    Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, MN 55905, USA
    Nat Cell Biol 11:204-10. 2009
    ..Mutation of Ser 1524 results in a defective decatenation checkpoint. These results reveal an important role of Topo II in checkpoint activation and in the maintenance of genomic stability...
  76. pmc Metnase mediates chromosome decatenation in acute leukemia cells
    Justin Wray
    Division of Hematology Oncology, Cancer Research and Treatment Center, Department of Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA
    Blood 114:1852-8. 2009
    ..Thus, Metnase expression levels may predict AML resistance to Topo IIalpha inhibitors, and Metnase is a potential therapeutic target for small molecule interference...
  77. pmc Topoisomerase II alpha expression and the benefit of adjuvant chemotherapy for postoperative patients with non-small cell lung cancer
    Shi Yan
    Department of Medical Oncology, General Hospital of CPLA, No 28 Fuxing road, Beijing, China
    BMC Cancer 10:621. 2010
    ..The objective of this study was to explore the possible prognostic and predictive role of topoisomerase II alpha (TopIIα) expression level in postoperative NSCLC patients who received adjuvant chemotherapy...
  78. pmc Quantification and clinical relevance of gene amplification at chromosome 17q12-q21 in human epidermal growth factor receptor 2-amplified breast cancers
    Pierre Jean Lamy
    Laboratoire de Biologie Spécialisée et d Oncogénétique, Centre régional de lutte contre le cancer Val d Aurelle Paul Lamarque, 208, rue des Apothicaires, Montpellier F 34298, France
    Breast Cancer Res 13:R15. 2011
    ..The aim of this study was to quantify the amplification of genes located on chromosome 17q and to analyze the relations between the pattern of gene amplifications and the patients' characteristics and survival...
  79. pmc TOP2A amplification in the absence of that of HER-2/neu: toward individualization of chemotherapeutic practice in breast cancer
    Ronan W Glynn
    Department of Surgery, Clinical Science Institute, National University of Ireland, Galway, Ireland
    Oncologist 16:949-55. 2011
    To investigate the relationship between human epidermal growth factor receptor (HER)-2/neu and the gene encoding topoisomerase IIα (TOP2A) in breast cancer, while elucidating their association with clinicopathological variables.
  80. doi Tumor topoisomerase II alpha status and response to anthracycline-based neoadjuvant chemotherapy in breast cancer
    Alíz Nikolényi
    Department of Oncotherapy, University of Szeged, Szeged, Hungary
    Oncology 80:269-77. 2011
    ..Anthracyclines target the enzyme topoisomerase IIα (TOP2A)...
  81. pmc Translational control of TOP2A influences doxorubicin efficacy
    Subramanya Srikantan
    Laboratory of Molecular Biology and Immunology, NIA IRP, NIH, Baltimore, Maryland 21224, USA
    Mol Cell Biol 31:3790-801. 2011
    The cellular abundance of topoisomerase IIα (TOP2A) critically maintains DNA topology after replication and determines the efficacy of TOP2 inhibitors in chemotherapy...
  82. doi HER2 and TOP2A as predictive markers for anthracycline-containing chemotherapy regimens as adjuvant treatment of breast cancer: a meta-analysis of individual patient data
    Angelo Di Leo
    Medical Oncology Unit, Hospital of Prato, Istituto Toscano Tumori, Prato, Italy
    Lancet Oncol 12:1134-42. 2011
    ..We aimed to assess the value of HER2 and TOP2A as predictive markers of response to anthracycline-based adjuvant therapy in patients with early breast cancer.
  83. ncbi Prognostic value of TOP2A gene amplification and chromosome 17 polysomy in early breast cancer
    Anna Zaczek
    Laboratory of Cell Biology, Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Debinki 1, 80 211, Gdansk, Poland
    Pathol Oncol Res 18:885-94. 2012
    The aim of this study was to analyze the occurrence of TOP2A gene amplification and chromosome 17 polysomy in patients with early breast cancer and to correlate the status of these alterations with the prognostic significance expressed ..
  84. pmc TOP2A RNA expression and recurrence in estrogen receptor-positive breast cancer
    Joseph A Sparano
    Weiler Division, Montefiore Einstein Cancer Center, Albert Einstein College of Medicine, Montefiore Medical Center, 1825 Eastchester Rd, Bronx, NY 10461, USA
    Breast Cancer Res Treat 134:751-7. 2012
    The purpose of this study is to evaluate the relationship between TOP2A RNA expression and recurrence in patients with operable estrogen receptor (ER)-positive breast cancer...
  85. pmc The structure of DNA-bound human topoisomerase II alpha: conformational mechanisms for coordinating inter-subunit interactions with DNA cleavage
    Timothy J Wendorff
    Biophysics Graduate Program, University of California Berkeley, Berkeley, CA 94720, USA
    J Mol Biol 424:109-24. 2012
    ..9-Å-resolution structure of the DNA cleavage core of human topoisomerase IIα (TOP2A) bound to a doubly nicked, 30-bp duplex oligonucleotide...
  86. ncbi Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively
    K B Tan
    Research and Development, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406
    Cancer Res 52:231-4. 1992
    ..We confirmed the chromosomal location of topo II alpha and mapped the topo II beta gene to chromosome 3. In addition, topo II beta exhibits genetic polymorphism as has been reported for topoisomerases I and II alpha...
  87. ncbi Histone deacetylase interacts directly with DNA topoisomerase II
    S C Tsai
    H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    Nat Genet 26:349-53. 2000
    ..Our results indicate the existence of a functionally coupled complex between these two enzymes and offer insights into the potential mechanisms of action of both enzymes...
  88. ncbi DNA cleavage and religation by human topoisomerase II alpha at high temperature
    K D Bromberg
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 40:8410-8. 2001
    ..Finally, similar high-temperature alterations were observed for yeast topoisomerase II and human topoisomerase IIbeta, suggesting that parallel heat-induced transitions may be widespread among type II topoisomerases...
  89. pmc Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite array
    Jennifer M Spence
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    EMBO J 21:5269-80. 2002
    ....
  90. ncbi A human topoisomerase II alpha heterodimer with only one ATP binding site can go through successive catalytic cycles
    Camilla Skouboe
    Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, Arhus C 8000, Denmark
    J Biol Chem 278:5768-74. 2003
    ..The results can be explained in a model, where efficient catalysis requires an extensive communication between the second ATP and the DNA segment to be cleaved...
  91. ncbi Modification of topoisomerase genes copy number in newly diagnosed childhood acute lymphoblastic leukemia
    E Guerin
    Laboratoire de Biochimie et de Biologie Moléculaire Hôpital de Hautepierre, Strasbourg, France
    Leukemia 17:532-40. 2003
    ..At diagnosis, allelic imbalance at topo-isomerase IIalpha (TOP2A) gene locus was found in 75% of informative cases whereas topoisomerase I and IIbeta gene loci are altered in none ..
  92. ncbi Human topoisomerase IIalpha rapidly relaxes positively supercoiled DNA: implications for enzyme action ahead of replication forks
    A Kathleen McClendon
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 280:39337-45. 2005
    ..These properties suggest that human topoisomerase IIalpha has the potential to alleviate torsional stress ahead of replication forks in an efficient and safe manner...
  93. doi Plk1-dependent phosphorylation regulates functions of DNA topoisomerase IIalpha in cell cycle progression
    Hongchang Li
    Department of Biochemistry and the Cancer Center, Purdue University, 175 S University Street, West Lafayette, IN 47907, USA
    J Biol Chem 283:6209-21. 2008
    ....
  94. pmc Outcome of patients with early-stage breast cancer treated with doxorubicin-based adjuvant chemotherapy as a function of HER2 and TOP2A status
    Raymond Tubbs
    Department of Molecular Pathology, Cleveland Clinic, Cleveland, OH 44195, USA
    J Clin Oncol 27:3881-6. 2009
    Amplification and deletion of the TOP2A gene have been reported as positive predictive markers of response to anthracycline-based therapy...
  95. ncbi Expression of E-cadherin, beta-catenin and topoisomerase IIalpha in leiomyosarcomas
    Pinelopi N Gogou
    Department of Radiotherapy, University of Ioannina, Medical School, Ioannnina, Greece
    Clin Transl Oncol 11:548-51. 2009
    ..The expression of E-cadherin, beta-catenin and topoisomerase II has been associated with clinical outcome of several cancers including sarcomas. We aimed to evaluate the expression of these markers in leiomyosarcomas (LMS)...
  96. pmc Mitotic chromosomes are constrained by topoisomerase II-sensitive DNA entanglements
    Ryo Kawamura
    Department of Physics, University of Illinois at Chicago, Chicago, IL 60607, USA
    J Cell Biol 188:653-63. 2010
    ..Our experiments show that DNA entanglements act in concert with protein-mediated compaction to fold chromatin into mitotic chromosomes...
  97. pmc Alteration of topoisomerase II-alpha gene in human breast cancer: association with responsiveness to anthracycline-based chemotherapy
    Michael F Press
    Norris Comprehensive Cancer Center, University of Southern California, CA, USA
    J Clin Oncol 29:859-67. 2011
    Approximately 35% of HER2-amplified breast cancers have coamplification of the topoisomerase II-alpha (TOP2A) gene encoding an enzyme that is a major target of anthracyclines...
  98. pmc Assessment of Topoisomerase II α status in breast cancer by quantitative PCR, gene expression microarrays, immunohistochemistry, and fluorescence in situ hybridization
    Atocha Romero
    Department of Medical Oncology, Hospital Clinico San Carlos, Madrid, Spain
    Am J Pathol 178:1453-60. 2011
    Anthracyclines are frequently used for the treatment of breast cancer and topoisomerase II alpha (TOP2A) is considered to be the molecular target...
  99. doi Lack of independent prognostic and predictive value of centromere 17 copy number changes in breast cancer patients with known HER2 and TOP2A status
    Kirsten Vang Nielsen
    Dako A S, Glostrup, Produktionsvej 42, DK 2600 Glostrup, Denmark
    Mol Oncol 6:88-97. 2012
    The clinical benefit of anthracyclines has been connected to HER2 status, TOP2A status and centromere 17 copy numbers (CEN-17)...
  100. doi Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells
    Yang Hong
    Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang 110004, China
    Neurosci Lett 518:138-43. 2012
    ..Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas...
  101. pmc Prognostic significance of TOP2A gene dosage in HER-2-negative breast cancer
    Anna J Zaczek
    Laboratory of Cell Biology, Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Debinki 1, 80 211 Gdansk, Poland
    Oncologist 17:1246-55. 2012
    ..growth factor receptor 2 (HER-2) gene alterations analyzed separately and jointly with topoisomerase II α (TOP2A) gene alterations; however, the role of TOP2A gene abnormalities alone has not been thoroughly investigated...

Research Grants18

  1. FUNCTIONAL AND MECHANISTIC STUDIES OF DNA TOPOISOMERASES
    Leroy Liu; Fiscal Year: 2001
    ..Our preliminary studies have demonstrated that the two human DNA TOP2 isoforms, TOP2a and TOP2b, can be activated to become DNA cleaving "nucleases" by hydrogen peroxide, a reactive oxygen species (..
  2. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
    ..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
  3. Matthew J O'Connell; Fiscal Year: 2015
    ..Moreover, Topoisomerase II (Top2) functions in cohesion and condensation, in addition to its well-defined roles in chromosome decatenation and ..
  4. Mechanism of action of TOP2-directed anticancer drugs
    Leroy F Liu; Fiscal Year: 2012
    The long-term goal of this application is to understand the mechanism of action of topoisomerase II (Top2)-targeting drugs...
  5. John L Nitiss; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Topoisomerase II (Top2) is the target of a wide range of active anti-cancer agents...
  6. Chemical Glycobiology on Anthracyclines
    Peng Wang; Fiscal Year: 2009
    ..enhanced efficacy towards anthracycline-resistant cancer cells and different selectivity against topoisomerase 2 (Top2) and Top1 targets...
  7. Diane J Mathis; Fiscal Year: 2015
    ..the hypothesis, that it promotes the recruitment and/or effectiveness of a complex (including DNA-PK, PARP1, TOP2, FACT, H2AX) responsible for disassembling and re-assembling nucleosomes as the transcriptional machinery ..
  8. DNA TOPOISOMERASE II - DOMAINS, BINDING SITES & FUNCTION
    STEPHEN WORLAND; Fiscal Year: 1993
    ..Regions of the protein required for DNA relaxation will be defined by deletion analysis of the TOP2 gene...
  9. Howard D Strickler; Fiscal Year: 2016
    ..activity / proliferation (p16/ki-67 cytology;CINtec+), (iii) cellular markers of aberrant S-phase induction (MCM2/Top2A cytology;BD ProExC), (iv) oncHPV E6/E7 oncogene mRNA expression (PreTect HPV-Proofer), and (v) an "in-house"HPV ..
  10. Topoisomerase-Associated Genome Instability
    SABRINA COTE; Fiscal Year: 2013
    ..cerevisiae. In particular, I'm analyzing how disruption of the topoisomerases Top1 and Top2 (by both mutation and by drug inhibition) induces mitotic DNA recombination...
  11. Smt4 Regulation of Centromere Function and Cohesion
    Jeff Bachant; Fiscal Year: 2007
    ..Finally, we have isolated DNA topoisomerase II (Top2) as a Smt3/SUMO-1 substrate that can suppress the Smt4 cohesion defect...
  12. Akt1 and erbB2 - New Molecular Targets for Hormone Resistance in Breast Cancer
    Adriana Stoica; Fiscal Year: 2007
    ..Therefore, a subset of 18 genes: ANXA6, ARC, EIF5A, ASCL1, HES1, EGR1, EGR3, FOS, MYC, MYB, SMAD7, TGF-b1, QRT1, TOP2A, TXNIP, CYR61, and CTGF (identified by gene array experiments) that are regulated by both estrogen and Akt1 may ..
  13. EXPLORING THE INTERACTION BETWEEN SGS1 AND TOP3
    Marisa Wagner; Fiscal Year: 2004
    ..In addition to Top3, yeast also contain the Top1 and Top2 topoisomerases...
  14. DNA REPAIR AND ANTITOPOISOMERASE DRUG EFFECTS
    John L Nitiss; Fiscal Year: 2010
    ..the nuclease activities of several repair complexes are important following cell survival following exposure to Top2 targeting agents...
  15. p16 and HPV in Low-Grade Cervical Cytologic Specimens
    KENNETH SHROYER; Fiscal Year: 2007
    ..abstract_text> ..
  16. Type II TGF Beta Receptor and RCC Progression
    Alexander Parker; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  17. Molecular Determinants of Mitochondrial Optic Atrophy
    SUSAN CLINE; Fiscal Year: 2005
    ..abstract_text> ..
  18. Dendritic Cell Immunotherapy for Cervical Cancer
    Alessandro Santin; Fiscal Year: 2004
    ..This study will determine whether E7 antigen-loaded autologous DC vaccination offers the potential of therapeutic benefit for patients with HPV16 or HPV18-associated cervical cancer. ..