SUMO-1

Summary

Gene Symbol: SUMO-1
Description: small ubiquitin-like modifier 1
Alias: DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1, small ubiquitin-related modifier 1, GAP modifying protein 1, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, sentrin, ubiquitin-homology domain protein PIC1, ubiquitin-like protein SMT3C, ubiquitin-like protein UBL1
Species: human

Top Publications

  1. pmc SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage
    Y Mao
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 97:4046-51. 2000
  2. ncbi Sequential modification of NEMO/IKKgamma by SUMO-1 and ubiquitin mediates NF-kappaB activation by genotoxic stress
    Tony T Huang
    Department of Pharmacology, University of Wisconsin Madison, 301 SMI, 1300 University Avenue, Madison, WI 53706, USA
    Cell 115:565-76. 2003
  3. ncbi Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation
    Jing Song
    Division of Immunology and Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 280:40122-9. 2005
  4. ncbi SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation
    J M Desterro
    School of Biomedical Science, University of St Andrews, Fife, United Kingdom
    Mol Cell 2:233-9. 1998
  5. doi RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation
    Michael H Tatham
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nat Cell Biol 10:538-46. 2008
  6. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
  7. pmc The disruption of ND10 during herpes simplex virus infection correlates with the Vmw110- and proteasome-dependent loss of several PML isoforms
    R D Everett
    MRC Virology Unit, Glasgow G11 5JR, Scotland
    J Virol 72:6581-91. 1998
  8. ncbi A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
    R Mahajan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:97-107. 1997
  9. ncbi SUMO modification of Huntingtin and Huntington's disease pathology
    Joan S Steffan
    Department of Psychiatry and Human Behavior, Gillespie 2121, University of California, Irvine, CA 92697, USA
    Science 304:100-4. 2004
  10. pmc Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 287:42071-83. 2012

Research Grants

  1. Maria Lauda Tomasi; Fiscal Year: 2016
  2. STUDIES OF THE SENTRIN FAMILY OF UBIQUITIN-LIKE PROTEINS
    Edward Yeh; Fiscal Year: 2000
  3. Michael J Matunis; Fiscal Year: 2016
  4. p90RSK: A flow responsive mediator of inflammation
    Keigi Fujiwara; Fiscal Year: 2013
  5. Smt4 Regulation of Centromere Function and Cohesion
    Jeff Bachant; Fiscal Year: 2007
  6. Characterization of Fortilin, A Novel Anti-p53 Protein
    Kenichi Fujise; Fiscal Year: 2005
  7. Activity based probes that target desumoylating enzymes
    Douglas Cyr; Fiscal Year: 2009
  8. Role of SENP1 in Prostate Cancer Pathogenesis
    Tasneem Bawa Khalfe; Fiscal Year: 2007
  9. Development of a High-Throughput Assay for Human Desumoylating Enzymes [SENPs]
    Guy Salvesen; Fiscal Year: 2007
  10. Gene Regulation by and of LEDGF
    Dhirendra P Singh; Fiscal Year: 2012

Detail Information

Publications298 found, 100 shown here

  1. pmc SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage
    Y Mao
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 97:4046-51. 2000
    ..iii) TOP1 physically interacts with UBC9. (iv) Ubc9 mutant yeast cells expressing human DNA TOP1 was hypersensitive to CPT, suggesting that UBC9/SUMO-1 may be involved in the repair of TOP1-mediated DNA damage...
  2. ncbi Sequential modification of NEMO/IKKgamma by SUMO-1 and ubiquitin mediates NF-kappaB activation by genotoxic stress
    Tony T Huang
    Department of Pharmacology, University of Wisconsin Madison, 301 SMI, 1300 University Avenue, Madison, WI 53706, USA
    Cell 115:565-76. 2003
    ..These SUMO and ubiquitin modification pathways may serve as anticancer drug targets...
  3. ncbi Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation
    Jing Song
    Division of Immunology and Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 280:40122-9. 2005
    ..This study provides a clear mechanism of the SBM sequence variations and its recognition of the SUMO moiety in sumoylated proteins...
  4. ncbi SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation
    J M Desterro
    School of Biomedical Science, University of St Andrews, Fife, United Kingdom
    Mol Cell 2:233-9. 1998
    ..Thus, while ubiquitination targets proteins for rapid degradation, SUMO-1 modification acts antagonistically to generate proteins resistant to degradation...
  5. doi RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation
    Michael H Tatham
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nat Cell Biol 10:538-46. 2008
    ..These results demonstrate that poly-SUMO chains can act as discrete signals from mono-SUMOylation, in this case targeting a poly-SUMOylated substrate for ubiquitin-mediated proteolysis...
  6. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
    ..Moreover, PIASy binds to nuclear matrix-associated DNA sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated subnuclear sequestration accounts for the repression of LEF1 activity...
  7. pmc The disruption of ND10 during herpes simplex virus infection correlates with the Vmw110- and proteasome-dependent loss of several PML isoforms
    R D Everett
    MRC Virology Unit, Glasgow G11 5JR, Scotland
    J Virol 72:6581-91. 1998
    ..Recently, a ubiquitin-specific protease enzyme (named HAUSP) and a ubiquitin-homology family protein (PIC1) have been found associated with ND10...
  8. ncbi A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
    R Mahajan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:97-107. 1997
    ..Inhibition of nuclear protein import resulting from antibodies directed at NPC-associated RanGAP1 cannot be overcome by soluble cytosolic RanGAP1, indicating that GTP hydrolysis by Ran at RanBP2 is required for nuclear protein import...
  9. ncbi SUMO modification of Huntingtin and Huntington's disease pathology
    Joan S Steffan
    Department of Psychiatry and Human Behavior, Gillespie 2121, University of California, Irvine, CA 92697, USA
    Science 304:100-4. 2004
    ..Lysine mutations that prevent both SUMOylation and ubiquitination of Httex1p reduce HD pathology, indicating that the contribution of SUMOylation to HD pathology extends beyond preventing Htt ubiquitination and degradation...
  10. pmc Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 287:42071-83. 2012
    ..We suggest that the clustered SIMs in these proteins form distinct SUMO binding domains to recognize diverse forms of protein sumoylation...
  11. doi Phospho-regulated SUMO interaction modules connect the SUMO system to CK2 signaling
    Per Stehmeier
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Mol Cell 33:400-9. 2009
    ..CK2-regulated phosphoSIM modules were also dissected in the tumor suppressor PML and the exosome component PMSCL1, indicating that these modules serve as general platforms that integrate CK2- and SUMO-regulated signaling networks...
  12. pmc SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes
    Delphine Cuchet-Lourenço
    MRC University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom
    PLoS Pathog 7:e1002123. 2011
    ..We conclude that recruitment of ND10 components to sites associated with HSV-1 genomes reflects a cellular defence against invading pathogen DNA that is regulated through the SUMO modification pathway...
  13. pmc Modification of the human thymine-DNA glycosylase by ubiquitin-like proteins facilitates enzymatic turnover
    Ulrike Hardeland
    Institute of Medical Radiobiology, University of Zürich and the Paul Scherrer Institute, August Forel Strasse 7, Ch 8008 Zurich, Switzerland
    EMBO J 21:1456-64. 2002
    ..These observations implicate a function of sumoylation in the controlled dissociation of TDG from the AP site and open up novel perspectives for the understanding of the molecular mechanisms coordinating the early steps of BER...
  14. ncbi Structure determination of the small ubiquitin-related modifier SUMO-1
    P Bayer
    Abteilung Physikalische Biochemie, Max Planck Institut fur molekulare Physiologie, Dortmund, Germany
    J Mol Biol 280:275-86. 1998
    ....
  15. ncbi Crystal structure of thymine DNA glycosylase conjugated to SUMO-1
    Daichi Baba
    Graduate School of Integrated Science, Yokohama City University, Yokohama 230 0045, Japan
    Nature 435:979-82. 2005
    ..This helix is formed by covalent and non-covalent contacts between TDG and SUMO-1. The non-covalent contacts are also essential for release from the product DNA, as verified by mutagenesis...
  16. pmc SUMO-1 modification activates the transcriptional response of p53
    M S Rodriguez
    School of Biomedical Science, BMS Building, University of St Andrews, St Andrews, Fife KY16 9ST
    EMBO J 18:6455-61. 1999
    ..The SUMO-1 modification pathway therefore acts as a potential regulator of the p53 response and may represent a novel target for the development of therapeutically useful modulators of the p53 response...
  17. ncbi Small ubiquitin-like modifier (SUMO) modification of natively unfolded proteins tau and alpha-synuclein
    Veronique Dorval
    Department of Medical Biophysics and Centre for Research in Neurodegenerative Diseases, University of Toronto, 6 Queen s Park Crescent West, Toronto, Ontario M5S 3H2, Canada
    J Biol Chem 281:9919-24. 2006
    ..These findings revealed a new, possibly regulatory, modification of tau and alpha-synuclein that may also have implications for their pathogenic roles in neurodegenerative diseases...
  18. doi SUMOylation mediates the nuclear translocation and signaling of the IGF-1 receptor
    Bita Sehat
    1Department of Oncology and Pathology, Karolinska Institutet, Cancer Center Karolinska, R8 04, Karolinska University Hospital, SE 171 76 Stockholm, Sweden
    Sci Signal 3:ra10. 2010
    ..Thus, we demonstrate a SUMOylation-mediated mechanism of IGF-1R signaling that has potential implications for gene regulation...
  19. pmc Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions
    Sarah Schulz
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Heidelberg, Germany
    EMBO Rep 13:930-8. 2012
    ..Thus, USPL1 represents a third type of SUMO protease, with essential functions in Cajal body biology...
  20. pmc Small ubiquitin-like modifier 1-3 conjugation [corrected] is activated in human astrocytic brain tumors and is required for glioblastoma cell survival
    Wei Yang
    Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA
    Cancer Sci 104:70-7. 2013
    ....
  21. doi Small ubiquitin-related modifier (SUMO) pathway-mediated enhancement of human cytomegalovirus replication correlates with a recruitment of SUMO-1/3 proteins to viral replication compartments
    Myriam Scherer
    Institute for Clinical and Molecular Virology, University of Erlangen Nuremberg, Schlossgarten 4, 91054 Erlangen, Germany
    J Gen Virol 94:1373-84. 2013
    ....
  22. ncbi SUMOylation of the polyglutamine repeat protein, ataxin-1, is dependent on a functional nuclear localization signal
    Brigit E Riley
    Department of Biochemistry, Molecular Biology, Biophysics, Institute of Human Genetics, The University of Minnesota, Minneapolis, MN 55455, USA
    J Biol Chem 280:21942-8. 2005
    ..Lys(16), Lys(194) preceding the polyglutamine tract, Lys(610)/Lys(697) in the C-terminal ataxin high mobility group domain, and Lys(746) all contribute to ataxin-1 SUMOylation...
  23. pmc Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies
    A L Adamson
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Virol 75:2388-99. 2001
    ..These results suggest that disruption of PML bodies is important for efficient lytic replication of EBV. Furthermore, Z may potentially alter the function of a variety of cellular proteins by inhibiting SUMO-1 modification...
  24. doi Synergistic activation of the human MnSOD promoter by DJ-1 and PGC-1alpha: regulation by SUMOylation and oxidation
    Nan Zhong
    Department of Neurology, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Hum Mol Genet 17:3357-67. 2008
    ..Consequent abnormal mitochondrial gene expression may contribute to the development of sporadic PD...
  25. doi SUMOylation regulates Kv2.1 and modulates pancreatic beta-cell excitability
    Xiao Qing Dai
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, T6G 2E1 Canada
    J Cell Sci 122:775-9. 2009
    ..Thus, protein SUMOylation can exert a strong inhibitory action on the voltage-dependent K(+) channel Kv2.1 and can regulate cellular excitability in native beta-cells...
  26. pmc Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1
    Ville Hietakangas
    Turku Centre for Biotechnology, University of Turku and Abo Akademi University and Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Mol Cell Biol 23:2953-68. 2003
    ..A transient phosphorylation-dependent colocalization of HSF1 and SUMO-1 in nuclear stress granules provides evidence for a strictly regulated subnuclear interplay between HSF1 and SUMO...
  27. pmc Interaction of DJ-1 with Daxx inhibits apoptosis signal-regulating kinase 1 activity and cell death
    Eunsung Junn
    Center for Neurodegenerative and Neuroimmunologic Diseases, Department of Neurology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 102:9691-6. 2005
    ..These findings suggest that the regulated sequestration of Daxx in the nucleus and keeping apoptosis signal-regulating kinase 1 activation in check is a critical mechanism by which DJ-1 exerts its cytoprotective function...
  28. doi Sumoylation of specificity protein 1 augments its degradation by changing the localization and increasing the specificity protein 1 proteolytic process
    Yi Ting Wang
    Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 701, Taiwan
    J Mol Biol 380:869-85. 2008
    ..This interaction increased the efficiency of Sp1 proteolytic processing and ubiquitination and then resulted in Sp1 degradation. Therefore, sumoylation of Sp1 is attenuated during tumorigenesis in order to increase Sp1 stability...
  29. pmc A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells
    Frederic Galisson
    CNRS FRE3238, Institut Andre Lwoff, Villejuif, France
    Mol Cell Proteomics 10:M110.004796. 2011
    ....
  30. pmc SUMO1-dependent modulation of SERCA2a in heart failure
    Changwon Kho
    Cardiovascular Research Center, Mount Sinai School of Medicine, 1 Gustave L Levy Place, Box 1030, New York, New York 10029, USA
    Nature 477:601-5. 2011
    ..Taken together, our data show that SUMOylation is a critical post-translational modification that regulates SERCA2a function, and provide a platform for the design of novel therapeutic strategies for heart failure...
  31. pmc SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex
    M J Matunis
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York 10021, USA
    J Cell Biol 140:499-509. 1998
    ..This nuclear localization signal, and the presence of nine leucine-rich nuclear export signal motifs, suggests that RanGAP1 may shuttle between the nucleus and the cytoplasm...
  32. ncbi SUMO-1 modification activated GATA4-dependent cardiogenic gene activity
    Jun Wang
    Center for Cardiovascular Development, Division of Cardiovascular Sciences, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 279:49091-8. 2004
    ..GATA4 was identified as a SUMO-1-targeted transcription factor and together with PIAS1 was shown to be a potent regulator of cardiac gene activity...
  33. ncbi Regulation of heat shock transcription factor 1 by stress-induced SUMO-1 modification
    Y Hong
    Department of Biochemistry, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA
    J Biol Chem 276:40263-7. 2001
    ..Mutation of lysine 298 also results in a significant decrease in stress-induced transcriptional activity of HSF1 in vivo. This work implicates SUMO-1 modification as an important modulator of HSF1 function in response to stress...
  34. ncbi Pc2-mediated sumoylation of Smad-interacting protein 1 attenuates transcriptional repression of E-cadherin
    Jianyin Long
    Molecular Oncology Group, McGill University, Montreal, Quebec H3A 1A1, Canada
    J Biol Chem 280:35477-89. 2005
    ..Thus SIP1 sumoylation regulates its transcriptional activity in a promoter context-dependent manner and may represent an important intervention target to modulate EMT in tumorigenesis...
  35. ncbi Specification of SUMO1- and SUMO2-interacting motifs
    Christina Maria Hecker
    Institute for Biochemistry II, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    J Biol Chem 281:16117-27. 2006
    ....
  36. pmc Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks
    Yaron Galanty
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 462:935-9. 2009
    ..These findings thus identify PIAS1 and PIAS4 as components of the DDR and reveal how protein recruitment to DSB sites is controlled by coordinated SUMOylation and ubiquitylation...
  37. pmc SUMO-targeted ubiquitin E3 ligase RNF4 is required for the response of human cells to DNA damage
    Yili Yin
    Wellcome Trust Centre for Gene Regulation and Expression, Dundee, United Kingdom
    Genes Dev 26:1196-208. 2012
    ..Thus, RNF4 is a novel DNA damage-responsive protein that plays a role in homologous recombination and integrates SUMO modification and ubiquitin signaling in the cellular response to genotoxic stress...
  38. pmc The mechanisms of PML-nuclear body formation
    Tian Huai Shen
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell 24:331-9. 2006
    ....
  39. ncbi DJ-1 transcriptionally up-regulates the human tyrosine hydroxylase by inhibiting the sumoylation of pyrimidine tract-binding protein-associated splicing factor
    Nan Zhong
    Department of Neurology, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    J Biol Chem 281:20940-8. 2006
    ..Therefore, our results suggest DJ-1 as a regulator of protein sumoylation and directly link the loss of DJ-1 expression and transcriptional dysfunction to impaired dopamine synthesis...
  40. ncbi SMT3A, a human homologue of the S. cerevisiae SMT3 gene, maps to chromosome 21qter and defines a novel gene family
    V Lapenta
    Institute of Medical Genetics, Catholic University, Rome, Italy
    Genomics 40:362-6. 1997
    ..The predicted protein of 103 amino acids appears to be a homologue of the Saccharomyces cerevisiae SMT3 protein, whose gene was previously isolated as a suppressor of mutations in the MIF2 gene...
  41. ncbi Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9
    M H Tatham
    Institute of Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 5ST, United Kingdom
    J Biol Chem 276:35368-74. 2001
    Conjugation of the small ubiquitin-like modifier SUMO-1/SMT3C/Sentrin-1 to proteins in vitro is dependent on a heterodimeric E1 (SAE1/SAE2) and an E2 (Ubc9)...
  42. ncbi Modification of Daxx by small ubiquitin-related modifier-1
    Moon Sun Jang
    Research Center for Biomedicinal Resources and Division of Life Science, Paichai University, 439 6 Doma 2 Dong, Seo Gu, Daejon 302 735, Republic of Korea
    Biochem Biophys Res Commun 295:495-500. 2002
    ..Daxx (K630, 631A), the sumoylation defective mutant, was able to interact with PML and co-localized with PML in the PML oncogenic domains (PODs). Thus, our data show that sumoylation status of Daxx does not affect its presence in PODs...
  43. ncbi Non-covalent interaction between nucleocapsid protein of Tula hantavirus and small ubiquitin-related modifier-1, SUMO-1
    Pasi Kaukinen
    Department of Virology, Haartman Institute, PO Box 21, University of Helsinki, FIN 00014, Helsinki, Finland
    Virus Res 92:37-45. 2003
    ..Five cDNA clones encoding SUMO-1 (Small Ubiquitin-related MOdifier, also known as sentrin) were selected in the LexA system...
  44. pmc Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins
    Joris Hemelaar
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:84-95. 2004
    ..The UBL probes described here will be valuable tools for the further characterization of the enzymatic pathways that govern modification by UBLs...
  45. ncbi Broad spectrum identification of cellular small ubiquitin-related modifier (SUMO) substrate proteins
    Yingming Zhao
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Biol Chem 279:20999-1002. 2004
    ....
  46. doi The SUMO modification pathway is involved in the BRCA1 response to genotoxic stress
    Joanna R Morris
    Department of Medical and Molecular Genetics, King s College London, Guy s Medical School Campus, London SE1 9RT, UK
    Nature 462:886-90. 2009
    ..These data demonstrate that the SUMOylation pathway plays a significant role in mammalian DNA damage response...
  47. pmc Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5
    Mauro W Costa
    Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia
    PLoS ONE 6:e24812. 2011
    ..We also observe SUMOylation of Nkx2-5 cofactors, which may be critical to Nkx2-5 regulation. Our data reveal highly complex regulatory mechanisms driven by SUMOylation to modulate Nkx2-5 activity...
  48. pmc RNF4-dependent hybrid SUMO-ubiquitin chains are signals for RAP80 and thereby mediate the recruitment of BRCA1 to sites of DNA damage
    Catherine M Guzzo
    Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Sci Signal 5:ra88. 2012
    ..Our findings, therefore, connect ubiquitin- and SUMO-dependent DSB recognition, revealing that RNF4-synthesized hybrid SUMO-ubiquitin chains are recognized by RAP80 to promote BRCA1 recruitment and DNA repair...
  49. pmc Multivalent interactions of the SUMO-interaction motifs in RING finger protein 4 determine the specificity for chains of the SUMO
    Kirstin Keusekotten
    Institute for Genetics, University of Cologne, 50674 Koln, Germany
    Biochem J 457:207-14. 2014
    ..In the present paper, we describe that the sequence and spacing of the SIMs (SUMO-interaction motifs) in RNF4 regulate the avidity-driven recognition of substrate proteins carrying SUMO chains of variable length...
  50. pmc Role of SUMO in RNF4-mediated promyelocytic leukemia protein (PML) degradation: sumoylation of PML and phospho-switch control of its SUMO binding domain dissected in living cells
    Yann Percherancier
    CNRS FRE2937, Institut Andre Lwoff, Villejuif 94801, France
    J Biol Chem 284:16595-608. 2009
    ....
  51. doi SUMOylation attenuates the aggregation propensity and cellular toxicity of the polyglutamine expanded ataxin-7
    Alexandre Janer
    INSERM, UMRS 975, CNRS UMR 7225, Paris, France
    Hum Mol Genet 19:181-95. 2010
    ..Our results demonstrate an influence of SUMOylation on the multistep aggregation process of ATXN7 and implicate a role for ATXN7 SUMOylation in SCA7 pathogenesis...
  52. pmc SUMO-interacting motifs of human TRIM5α are important for antiviral activity
    Gloria Arriagada
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, United States of America
    PLoS Pathog 7:e1002019. 2011
    ..We propose that at least a portion of the antiviral activity of TRIM5α is mediated through the binding of its SIMs to SUMO-conjugated CA...
  53. doi A functional SUMO-interacting motif in the transactivation domain of c-Myb regulates its myeloid transforming ability
    T Saether
    Department of Molecular Biosciences, University of Oslo, Oslo, Norway
    Oncogene 30:212-22. 2011
    ..This establishes SUMO binding as a mechanism involved in modulating the transactivation activity of c-Myb, and responsible for keeping the transforming potential of the oncoprotein in check...
  54. doi Structural and functional roles of Daxx SIM phosphorylation in SUMO paralog-selective binding and apoptosis modulation
    Che Chang Chang
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
    Mol Cell 42:62-74. 2011
    ..Our findings provide structural insights into the Daxx-SIM:SUMO-1 complex, a model of SIM phosphorylation-enhanced SUMO paralog-selective modification and interaction, and phosphorylation-regulated Daxx function in apoptosis...
  55. ncbi SUMO-1 conjugation to intact DNA topoisomerase I amplifies cleavable complex formation induced by camptothecin
    Koji Horie
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Japan
    Oncogene 21:7913-22. 2002
    ..These findings suggested that Topo1 inactivation by CPT treatment can trigger Topo1 sumoylation, leading to enhanced cleavable complex formation...
  56. pmc Noncovalent interaction between Ubc9 and SUMO promotes SUMO chain formation
    Puck Knipscheer
    Department of Molecular Carcinogenesis, The Netherlands Cancer Institute and Center for Biomedical Genetics, Plesmanlaan, Amsterdam, The Netherlands
    EMBO J 26:2797-807. 2007
    ..Structural comparison suggests a model for poly-sumoylation involving a mechanism analogous to Mms2-Ubc13-mediated ubiquitin chain formation...
  57. doi LYRIC/AEG-1 is targeted to different subcellular compartments by ubiquitinylation and intrinsic nuclear localization signals
    Hayley J Thirkettle
    Uro Oncology Research Group, Cancer Research UK
    Clin Cancer Res 15:3003-13. 2009
    ..LYRIC/AEG-1 contains three uncharacterized nuclear localization signals (NLS), which may regulate its distribution and, ultimately, function in cells...
  58. pmc SUMOylation modulates the function of Aurora-B kinase
    Gonzalo Fernández-Miranda
    Cell Division and Cancer Group, Spanish National Cancer Research Center CNIO, Madrid, Spain
    J Cell Sci 123:2823-33. 2010
    ..These data suggest that SUMOylation of Aurora B modulates its function, possibly by mediating the extraction of CPC complexes from chromosome arms during prometaphase...
  59. ncbi Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation
    Michael H Tatham
    Center for Biomolecular Sciences, University of St Andrews, St Andrews, Scotland
    Biochemistry 42:9959-69. 2003
    ..The conservation of E2 enzymes across the ubiquitin and ubiquitin-like protein pathways indicates that analogous N-terminal sites of E2 enzymes are likely to have similar roles in general...
  60. ncbi Characterization of the localization and proteolytic activity of the SUMO-specific protease, SENP1
    Daniel Bailey
    Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 OTL, UK
    J Biol Chem 279:692-703. 2004
    ..Finally, we demonstrate that SENP1 localization is influenced by expression and localization of SUMO-1-conjugated target proteins within the cell...
  61. pmc DNA repair factor XPC is modified by SUMO-1 and ubiquitin following UV irradiation
    Qi En Wang
    Department of Radiology, The Ohio State University 103 Wiseman Hall, 400 W 12th Avenue, Columbus, OH 43210, USA
    Nucleic Acids Res 33:4023-34. 2005
    ..Our results also suggest that at least one function of UV-induced XPC sumoylation is related to the stabilization of XPC protein...
  62. pmc SUMOylation of DEC1 protein regulates its transcriptional activity and enhances its stability
    Yongde Hong
    School of Life Science and Biotechnology, Dalian University of Technology, Dalian, China
    PLoS ONE 6:e23046. 2011
    ..These findings suggested that posttranslational modification of DEC1 in the form of SUMOylation may serve as a key factor that regulates the function of DEC1 in vivo...
  63. ncbi PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia
    M N Boddy
    Protein Structure Laboratory, Imperial Cancer Research Fund, London, UK
    Oncogene 13:971-82. 1996
    ..A human B-cell cDNA library was screened for PML-interacting clones and a single positive clone (PIC1) was isolated. The sequence of PIC1 shows 52% identity to a S...
  64. ncbi Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex
    L Gong
    Research Center for Cardiovascular Diseases, Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas Houston Health Science Center, 77030, USA
    FEBS Lett 448:185-9. 1999
    b>Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins...
  65. ncbi A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3
    Lalitha Subramanian
    Department of Pharmacology and Medical Scientist Training Program, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
    J Biol Chem 278:9134-41. 2003
    ..Our results indicate that SUMO modification of SC motifs provides a means to rapidly control higher order interactions among transcription factors and suggests that SUMOylation may be a general mechanism to limit transcriptional synergy...
  66. pmc Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1
    Zheng Xu
    Department of Biochemistry, Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong
    Biochem J 386:325-30. 2005
    ..To understand further this proteolytic processing, we expressed and purified SENP1 (sentrin-specific protease 1), one of the SUMO-specific proteases, using an Escherichia coli expression system...
  67. ncbi SUMO-1 promotes association of SNURF (RNF4) with PML nuclear bodies
    Marika Hakli
    Biomedicum Helsinki, Institute of Biomedicine, University of Helsinki, P O Box 63, FI 00014 Helsinki, Finland
    Exp Cell Res 304:224-33. 2005
    ..In sum, we have identified SNURF as a novel component in PML bodies and suggest that SUMO-1-facilitated sequestration into these nuclear domains regulates the transcriptional activity of SNURF...
  68. pmc Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex
    David Reverter
    Structural Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Nature 435:687-92. 2005
    ....
  69. ncbi Coordinated regulation of AIB1 transcriptional activity by sumoylation and phosphorylation
    Huijian Wu
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100083, China
    J Biol Chem 281:21848-56. 2006
    ....
  70. pmc Distinct in vivo dynamics of vertebrate SUMO paralogues
    Ferhan Ayaydin
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
    Mol Biol Cell 15:5208-18. 2004
    ....
  71. doi Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25
    Erik Meulmeester
    Department of Biochemistry I, Faculty of Medicine, Georg August University of Gottingen, Humboldtallee 23, 37073 Gottingen, Germany
    Mol Cell 30:610-9. 2008
    ..One mechanism for paralog-specific sumoylation may, thus, involve SIM-dependent recruitment of SUMO1 or SUMO2/3 thioester-charged Ubc9 to targets...
  72. ncbi The nuclear receptor interaction domain of GRIP1 is modulated by covalent attachment of SUMO-1
    Noora Kotaja
    Biomedicum Helsinki, Institute of Biomedicine, University of Helsinki and Helsinki University Central Hospital, FIN 00014 Helsinki, Finland
    J Biol Chem 277:30283-8. 2002
    ....
  73. ncbi PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor and repress androgen receptor-dependent transcription
    Tamotsu Nishida
    Division of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Japan
    J Biol Chem 277:41311-7. 2002
    ..Thus, PIAS1 and PIASxalpha modulate the AR-dependent transactivation, which, at least in part, can be attributed to their SUMO-E3 activity toward AR...
  74. ncbi Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation
    Fang Wei
    Department of Animal Biology, Mari Lowe Center for Comparative Oncology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:21551-60. 2007
    ..Therefore, sumoylation of Oct4 results in increased stability, DNA binding, and transactivation and provides an important mechanism to regulate Oct4 activity...
  75. ncbi Induction of the SUMO-specific protease 1 transcription by the androgen receptor in prostate cancer cells
    TASNEEM BAWA-KHALFE
    Research Center for Cardiovascular Diseases, Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center, Houston, TX 77030, USA
    J Biol Chem 282:37341-9. 2007
    ..reported that androgen receptor-dependent transcription is significantly elevated with expression of the human sentrin/SUMO-specific protease (SENP1) in the androgen-sensitive human prostate cancer cell line (LNCaP)...
  76. ncbi Repression of the Transactivating Capacity of the Oncoprotein PLAG1 by SUMOylation
    Frederik Van Dyck
    Laboratory for Molecular Oncology, Department for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 279:36121-31. 2004
    ..Nuclear distribution of PLAG1 was not measurably influenced. Our results suggest a direct repression of the transactivating capacity of the oncoprotein PLAG1 by SUMOylation...
  77. pmc A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4
    James X Du
    Division of Digestive Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 285:28298-308. 2010
    ..These results, therefore, illustrate a novel mechanism by which SUMO interaction modulates the activity of transcription factors...
  78. pmc SUMOylation target sites at the C terminus protect Axin from ubiquitination and confer protein stability
    Min Jung Kim
    Department of Genetics and Development, 701 W 168th St, Columbia University Medical Center, New York, NY 10032, USA
    FASEB J 22:3785-94. 2008
    ..Therefore, some other specific property of the C6 motif seems to reduce the interaction of Axin with Dvl-1...
  79. pmc SUMO interaction motifs in Sizn1 are required for promyelocytic leukemia protein nuclear body localization and for transcriptional activation
    Ginam Cho
    Department of Pathology, The Children s Hospital of Philadelphia, USA
    J Biol Chem 284:19592-600. 2009
    ..Taken together, our data indicate that the SIMs in Sizn1 are required for its PML-NB localization and for the full transcriptional co-activation function in BMP signaling...
  80. pmc Down-regulation of c-Fos/c-Jun AP-1 dimer activity by sumoylation
    Guillaume Bossis
    Institute of Molecular Genetics of Montpellier, UMR5535 IFR122, CNRS 1919, France
    Mol Cell Biol 25:6964-79. 2005
    ..This supports the idea that this modification does not constitute a final inactivation step that necessarily precedes protein degradation...
  81. pmc SUMO-1 modification alters ADAR1 editing activity
    Joana M P Desterro
    Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649 028 Lisboa, Portugal
    Mol Biol Cell 16:5115-26. 2005
    ..Moreover, modification of wild-type recombinant ADAR1 by SUMO-1 reduces the editing activity of the enzyme in vitro. Taken together these data suggest a novel role for sumoylation in regulating RNA-editing activity...
  82. ncbi Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner
    Øyvind Dahle
    Department of Biochemistry, University of Oslo, Norway
    Eur J Biochem 270:1338-48. 2003
    ..We propose that the negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on the sumoylation sites located here...
  83. ncbi Interferon-induced antiviral Mx1 GTPase is associated with components of the SUMO-1 system and promyelocytic leukemia protein nuclear bodies
    O G Engelhardt
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Freiburg, D 79008, Germany
    Exp Cell Res 271:286-95. 2001
    ..This suggests a role of PML NBs and SUMO-1 in the antiviral action of Mx1 and may allow us to discover novel functions of this large GTPase...
  84. ncbi SUMO-1 marks subdomains within glial cytoplasmic inclusions of multiple system atrophy
    D L Pountney
    Department of Human Physiology and Centre for Neuroscience, Flinders University, Adelaide, Australia
    Neurosci Lett 381:74-9. 2005
    ..In cell culture experiments, we found that the nuclear and perinuclear accumulation of SUMO-1 aggregates could be induced in glioma cells by chemical inhibition of proteasomal protein degradation...
  85. ncbi Effects of SUMO-1 upon Epstein-Barr virus BZLF1 function and BMRF1 expression
    Amy L Adamson
    Department of Biology, University of North Carolina at Greensboro, Greensboro, NC 27402, USA
    Biochem Biophys Res Commun 336:22-8. 2005
    ..Therefore, SUMO-1 modification of proteins appears to have an important role in EBV lytic replication...
  86. ncbi SUMO-1 marks the nuclear inclusions in familial neuronal intranuclear inclusion disease
    D L Pountney
    Department of Human Physiology and Centre for Neuroscience, Flinders University, South Australia 5042, Bedford Park, Australia
    Exp Neurol 184:436-46. 2003
    ..Our study suggests that recruitment of SUMO-1 modified proteins into insoluble nuclear inclusions and proteasomal dysfunction may be involved in the pathogenesis of NIs in familial NIID cases...
  87. ncbi Characterization of human herpesvirus 6 variant B immediate-early 1 protein modifications by small ubiquitin-related modifiers
    Annie Gravel
    Laboratory of Virology, Rheumatology and Immunology Research Center, Room T1 49, CHUL Research Center and Faculty of Medicine, Laval University, 2705 Laurier Blvd, Sainte Foy, Quebec, Canada G1V 4G2
    J Gen Virol 85:1319-28. 2004
    ..Lys-802 (K-802) of IE1 from HHV-6 variant B is the only target residue capable of conjugation to SUMO-1/SMT3C/Sentrin-1, SUMO-2/SMT3A/Sentrin-3 or SUMO-3/SMT3B/Sentrin-2 as determined by transfection and in vitro SUMOylation ..
  88. ncbi Post-translational modification of Rta of Epstein-Barr virus by SUMO-1
    Li Kwan Chang
    Faculty of Biological Medicine and Environmental Biology and Graduate Institute of Biochemistry, Kaohsiung Medical University, 100, Shih Chuan 1st Road, Kaohsiung, Taiwan
    J Biol Chem 279:38803-12. 2004
    ..These results indicate that sumoylation of Rta may be important in EBV lytic activation...
  89. ncbi Functional modulation of parkin through physical interaction with SUMO-1
    Ji Won Um
    Department of Biology, College of Science, Yonsei University, Seoul, Korea
    J Neurosci Res 84:1543-54. 2006
    ..Our findings suggest that the E3 ubiquitin ligase activity of parkin and its intracellular localization may be modulated through the SUMO-1 association...
  90. ncbi Dnmt3b, de novo DNA methyltransferase, interacts with SUMO-1 and Ubc9 through its N-terminal region and is subject to modification by SUMO-1
    E S Kang
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejun, 305 701, South Korea
    Biochem Biophys Res Commun 289:862-8. 2001
    ..We also confirmed the modification of Dnmt3b by SUMO-1 in vivo. These results suggest that sumoylation may constitute a regulation mechanism of Dnmt3b in vivo...
  91. ncbi Sumoylation of the progesterone receptor and of the steroid receptor coactivator SRC-1
    Anne Chauchereau
    INSERM U135, Hormones, Genes, and Reproduction, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre, France
    J Biol Chem 278:12335-43. 2003
    ..This observation suggests that among the many protein partners involved in steroid hormone-mediated gene regulation several are probably targets of SUMO-1 modification...
  92. ncbi The SUMO pathway is required for selective degradation of DNA topoisomerase IIbeta induced by a catalytic inhibitor ICRF-193(1)
    Sevim Isik
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    FEBS Lett 546:374-8. 2003
    ..When the SUMO conjugating enzyme Ubc9 was conditionally knocked out, the ICRF-induced degradation of topoisomerase IIbeta did not occur, suggesting that the SUMO modification pathway is essential for the degradation...
  93. ncbi Modification of promyelocytic leukemia zinc finger protein (PLZF) by SUMO-1 conjugation regulates its transcriptional repressor activity
    Soo Im Kang
    Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Korea
    J Biol Chem 278:51479-83. 2003
    ..These results demonstrate that PLZF is modified by SUMO-1 conjugation and that this modification regulates the biological functions of PLZF...
  94. ncbi Transcriptional activity of megakaryoblastic leukemia 1 (MKL1) is repressed by SUMO modification
    Koji Nakagawa
    Division of Cancer Gene Regulation, Research Section of Disease Control, Institute for Genetic Medicine, Hokkaido University, Sapporo 060 0815, Japan
    Genes Cells 10:835-50. 2005
    ..The combined results demonstrated that MKL1 is sumoylated and this modification represses transcriptional activity of MKL1...
  95. pmc SUMO-1-dependent allosteric regulation of thymine DNA glycosylase alters subnuclear localization and CBP/p300 recruitment
    Ryan D Mohan
    Department of Physiology and Pharmacology, Siebens Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6G 2V4
    Mol Cell Biol 27:229-43. 2007
    ..These findings suggest that TDG sumoylation promotes intramolecular interactions with amino- and carboxy-terminal SUMO-1 binding motifs that dramatically alter the biochemical properties and subcellular localization of TDG...
  96. ncbi NMR characterization of the energy landscape of SUMO-1 in the native-state ensemble
    Ashutosh Kumar
    Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India
    J Mol Biol 367:1480-93. 2007
    ..We speculate that this would have significant implications for the binding of diversity of targets by SUMO-1 for the variety of functions it is involved in...
  97. pmc ZNF198 stabilizes the LSD1-CoREST-HDAC1 complex on chromatin through its MYM-type zinc fingers
    Christian B Gocke
    Howard Hughes Medical Institute, Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS ONE 3:e3255. 2008
    ....
  98. doi The p150 subunit of CAF-1 causes association of SUMO2/3 with the DNA replication foci
    Junsuke Uwada
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, 2 39 1 Kurokami, Kumamoto 860 8555, Japan
    Biochem Biophys Res Commun 391:407-13. 2010
    ....
  99. pmc Role of SUMO-1 and SUMO interacting motifs in rhesus TRIM5α-mediated restriction
    Zana Lukic
    Department of Microbiology and Immunology Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
    Retrovirology 10:10. 2013
    ..Here, we examined whether SUMO expression and the SIM1 and SIM2 motifs in rhesus monkey TRIM5α (rhTRIM5α) are similarly important for Human Immunodeficiency Type 1 (HIV-) restriction...
  100. ncbi Research on screening and identification of proteins interacting with ataxin-3
    Lu Shen
    Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China
    Zhonghua Yi Xue Yi Chuan Xue Za Zhi 22:242-7. 2005
    ....
  101. pmc SUMO1 modification of NF-kappaB2/p100 is essential for stimuli-induced p100 phosphorylation and processing
    Jaya Vatsyayan
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 19014, USA
    EMBO Rep 9:885-90. 2008
    ..Together, these findings show the crucial role of SUMO1 modification in p100 processing and provide mechanistic insights into the participation of SUMO1 modification in the regulation of signal transduction...

Research Grants58

  1. Maria Lauda Tomasi; Fiscal Year: 2016
    ..If successfully completed, these studies should provide highly novel information on the role of sumoylation in the development of ALD and may provide novel therapeutic strategies, which is of high public health priority. ..
  2. STUDIES OF THE SENTRIN FAMILY OF UBIQUITIN-LIKE PROTEINS
    Edward Yeh; Fiscal Year: 2000
    b>Sentrin is a family of ubiquitin-like molecules which can be covalently attached to other cellular proteins. In mammalian cells, there are three sentrin proteins that are expressed in all tissues and appear to have overlapping function...
  3. Michael J Matunis; Fiscal Year: 2016
    ..Knowledge gained from these studies has the potential to lead to the development of new strategies for both the detection and for the treatment of human cancers. ..
  4. p90RSK: A flow responsive mediator of inflammation
    Keigi Fujiwara; Fiscal Year: 2013
    ..Our data show that p90RSK activation inhibits Sentrin/SUMO- specific proteases 2 (SENP2) de-SUMOylation activity and increases both p53 and ERK5-SUMOylation, which ..
  5. Smt4 Regulation of Centromere Function and Cohesion
    Jeff Bachant; Fiscal Year: 2007
    ..The central hypothesis underlying this proposal is that the ubiquitin-related Smt3/SUMO-1 protein modifier is a key regulator of these two aspects of centromere function...
  6. Characterization of Fortilin, A Novel Anti-p53 Protein
    Kenichi Fujise; Fiscal Year: 2005
    ..2). Finally, we will establish whether fortilin is covalently modified by sentrin and other ubiquitin-like molecules (Aim 1.3)...
  7. Activity based probes that target desumoylating enzymes
    Douglas Cyr; Fiscal Year: 2009
    ..This proposal seeks to generate unique tools to study and track the activity of the seven known human Senps (sentrin specific proteases - sentrin being a previous name for SUMO)...
  8. Role of SENP1 in Prostate Cancer Pathogenesis
    Tasneem Bawa Khalfe; Fiscal Year: 2007
    Our laboratory cloned the first human sentrin/SUMO-specific protease, SENP1. The isopeptidase activity of this enzyme deconjugates the posttranslation modification by SUMO (small ubiquitin-like modifier)...
  9. Development of a High-Throughput Assay for Human Desumoylating Enzymes [SENPs]
    Guy Salvesen; Fiscal Year: 2007
    ..clan CE is defined by seven proteases with a common fold and catalytic mechanism, commonly known as SENPs (sentrin specific proteases - sentrin being an alternative name for SUMO)...
  10. Gene Regulation by and of LEDGF
    Dhirendra P Singh; Fiscal Year: 2012
    ....
  11. The Induction of Interferon Regulatory Factor 7 Sumoylation by EBV LMP1
    Gretchen L Bentz; Fiscal Year: 2010
    ..Together, this project will provide insight into new role for LMP1 and IRF7 during EBV latency as well as potential targets for the regulation of anti-viral immune responses. ..
  12. Ubiquitin-dependent proteolysis: Specificity & Mechanism
    Keith D Wilkinson; Fiscal Year: 2010
    ..Second, a modern systems biology approach takes advantage of our synthesis of polyubiquitin chain analogs to identify ubiquitin-binding proteins with specificity for different ubiquitin domains and different architectures (Aim 3). ..
  13. ROBERT CHARLES AUGUSTINE; Fiscal Year: 2014
    ..Broadly, this work will provide a mechanistic basis for understanding how organisms can rapidly respond and defend themselves from environmental stress and pathogen invasion. ..
  14. Yuan Chen; Fiscal Year: 2015
    ..In preliminary studies, we also identified a role of SENP1 and SENP2 in HIV replication...
  15. Marianna Bei; Fiscal Year: 2014
    ....
  16. Roger J Hajjar; Fiscal Year: 2016
    ..We now propose to further characterize the molecular mechanisms of SUMO1 in regulating SERCA2a function and to evaluate the multiple pathways regulating SUMOylation of SERCA2a. ..
  17. Towards understanding the role of sumoylation in ion channel regulation
    Sindhu Rajan; Fiscal Year: 2011
    ..The Departments of Pediatrics, Neurobiology, Pharmacology and Physiology at the University of Chicago include an internationally recognized cadre of ion channel researchers. ..
  18. MARK W HOCHSTRASSER; Fiscal Year: 2016
    ..Ligation to each Ubl has unique mechanistic and functional consequences. SUMO (encoded by SMT3 in yeast) is a highly divergent Ubl...
  19. Shigeki Miyamoto; Fiscal Year: 2014
    ..Second, understanding the mechanisms of NF-kB activation by DNA damaging agents may help identify novel drug targets to improve the current methods of cancer therapy. ..
  20. Mechanisms of Neuronal Specific Transcription
    Grace Gill; Fiscal Year: 2009
    ....
  21. Changwon Kho; Fiscal Year: 2014
    ..In addition, the PI has designed a plan to evaluate the use of small molecule inhibitors of SERCA2a acetylation as an alternative approach towards creating novel classes of cardiotherapeutics. ..
  22. PcG Function of YY1 in Transcription and Development
    Michael L Atchison; Fiscal Year: 2010
    ..PcG proteins are also implicated in muscle differentiation. Therefore, we will test the roles of YY1, CtBP, SUMO-1, and PCNA in differentation of myblasts into myotubes. ..
  23. Estrogen receptor beta protein:protein interactions following estrogen withdrawal
    NATASHA MOTT; Fiscal Year: 2013
    ..Overall, this proposal will reveal molecular mechanisms occurring during estrogen withdrawal that may alter the brain's receptivity to HT. ..
  24. Davide Trotti; Fiscal Year: 2015
    ..The scope of this last aim could then be key, in particular if results of aim 1-2 establisha role of the astroglial CTE-SUMO1 fragment in motor neuron toxicity and ALS pathogenesis. ..
  25. FASEB CONFERENCE ON UBIQUITIN AND PROTEIN DEGRADATION
    Cecile Pickart; Fiscal Year: 1999
    ..In addition, there will be two poster sessions. The small size of the conference, and its format, are designed to foster interactions among the 165 participants. ..
  26. Toxicity of a SUMOylated Fragment of the Glial Glutamate Transporter EAAT2 in ALS
    Emily Foran; Fiscal Year: 2012
    ..Previous work in a co-culture model has indicated that CTE-SUMO1 accumulation within astrocyte nuclei is toxic to neighboring motor neurons. This question will be further examined in vivo using a novel transgenic mouse model. ..
  27. Pathogenic Impact of SUMO modification in Huntington's disease
    Leslie Michels Thompson; Fiscal Year: 2010
    ..Hypothesis 3: SUMO modification of Htt is critical to disease pathogenesis in vivo. Here we will test the physiologic relevance of SUMO modification pathways in Drosophila. ..
  28. EARLY INTERACTIONS OF DNA VIRUSES AND HOST
    Gerd Maul; Fiscal Year: 2005
    ....
  29. Analysis of regulation and function of the BLM helicase
    Nathan Ellis; Fiscal Year: 2003
    ..In Aim 4, we analyze the domain structure of BLM and the role of oligomerization in helicase activity and BS cell complementation. ..
  30. Functions of Ub-like Proteins and Proteases
    KEITH WILKINSON; Fiscal Year: 2009
    ..In the broadest sense, these studies will contribute to our understanding of UBL protein metabolism and guide development of inhibitors and drugs for the treatment of cancers and microbial infections. ..
  31. TEL, AML1, and TEL/AML1 in Hematopoiesis
    Giuseppina Nucifora; Fiscal Year: 2006
    ....
  32. MOLECULAR BASIS OF ACUTE PROMYELOCYTIC LEUKEMIA
    J Don Chen; Fiscal Year: 2004
    ..A better understanding of mechanism of APL at the molecular and cellular levels should lead to a more effective diagnosis and treatment of this disease, as well as providing implications for other malignancies. ..
  33. Role of Vif in HIV-1 Replication/AIDS
    David Kabat; Fiscal Year: 2001
    ..This work may unveil a novel target for drug development in AIDS. ..
  34. MAPKs Regulate Progesterone Receptors by SUMO Modulation
    CAROL LANGE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  35. Mechanisms of Mineralcorticoid Receptor Function
    David Pearce; Fiscal Year: 2005
    ..abstract_text> ..
  36. INTRACELLULAR SIGNALING BY INTERFERONS
    Michael David; Fiscal Year: 2006
    ..The investigation of the STAT1 independent mechanism by which IFNbeta prevents T cell apoptosis will elucidate additional IFNalpha/beta induced signaling pathways. ..
  37. UV INDUCIBLE INHIBITION IN MELANOMA
    ZE EV RONAI; Fiscal Year: 2005
    ..In all, our proposed studies will establish the mechanism underlying Sumo-conjugation to Mdm2, its significance to the biology of this oncogene and develop reagents that alter Mdm2 sumoylation. ..
  38. Regulation of HSF1 and HSF2 by SUMO-1 Modification
    KEVIN SARGE; Fiscal Year: 2007
    ..abstract_text> ..
  39. Ubiquitin-dependent regulation of PPARgamma
    Z Floyd; Fiscal Year: 2007
    ..Information obtained from examining the molecular mechanisms regulating PPARgamma protein levels may provide new therapeutic targets in treating obesity, type 2 diabetes, and related cardiovascular diseases. [unreadable] [unreadable]..
  40. Transcription-enhanced recombination in mammalian cells
    Zhiyuan Shen; Fiscal Year: 2006
    ..UBC9 is an essential enzyme in the sumoylation pathway that conjugates the ubiquitin-like protein UBL1/SUMO-1 to certain cellular proteins. UBC9 associates with RAD52 and RAD51...
  41. Models of Familial Parkinson's Disease: DJ-1 Knockouts
    Ted Dawson; Fiscal Year: 2005
    ....
  42. NMR STUDIES OF PROTEIN INTERACTIONS IN UBIQUITINATION
    Yuan Chen; Fiscal Year: 2002
    ..We will characterize the UBL1- UBC9 conjugate and its interactions with target proteins...
  43. PROTEOLYTIC SIGNALING BY POLYUBIQUITIN CHAINS
    Michael Matunis; Fiscal Year: 2006
    ..The results of this research will also shed new light on the mechanism of the 26S proteasome, which is the major entity for regulatory proteolysis in eukaryotic cells. ..
  44. DNA REPAIR SIGNALING BY NOVEL POLYUBIQUITIN CHAINS
    Michael Matunis; Fiscal Year: 2007
    ..abstract_text> ..
  45. High-Throughput Protein NMR Proteomics
    ATHAN SHAKA; Fiscal Year: 2006
    ..Incorporation of the optimized FDM algorithm into the NMRPipe software suite, supported by NIH, will allow its use in many laboratories. ..
  46. Peroxiredoxin 6 and Cataractogenesis
    Dhirendra P Singh; Fiscal Year: 2010
    ..The proposed studies will provide a sound scientific basis for developing an antioxidant-based therapy or combination of therapies for preventing cataractogenesis and age-associated degenerative diseases in general. ..
  47. LIPOCORTINS (ANNEXINS) AND METAL-INDUCED MUTAGENESIS
    Fusao Hirata; Fiscal Year: 2003
    ..Our proposed research will provide further insights to understanding of the molecular mechanisms of carcinogenesis, genetic instability and pathological diseases associated with the formation of mutations by heavy metals. ..
  48. Genetic Modifiers of Epstein-Barr Virus/Host Interactns
    AMY ADAMSON; Fiscal Year: 2003
    ..The results from this genetic screen will shed light onto the mechanisms by which EBV alters normal cellular functions, as well as how genetic predisposition may play a role in EBV-related diseases. ..
  49. PATHOBIOCHEMISTRY OF ACUTE PROMYELOCYTIC LEUKEMIA
    Edward Yeh; Fiscal Year: 2004
    ..We have shown that wild type PML, but not the PML-RARalpha fusion proteins, are covalently modified by the sentrin proteins...
  50. TENDON CELLS:INTERACTIONS & RESPONSES TO STRESS IN VITRO
    Albert Banes; Fiscal Year: 2004
    ..Knowledge of how motion acts at the mechanistic level may lead to drug strategies for upregulating cell division and matrix expression with or without motion in aged or disabled patients. ..
  51. Identification of Small Molecules for Reactivation of p53 Cancer Mutants
    Peter Kaiser; Fiscal Year: 2008
    ..The objective of this proposal is to identify small molecules that restore function to p53 cancer mutants and to develop these molecules into therapeutics to restore p53 function in tumors. [unreadable] [unreadable] [unreadable]..
  52. Regulation by Proteolysis-Independent Ubiquitination
    Peter Kaiser; Fiscal Year: 2007
    ..This proposal aims to achieve detailed mechanistic insight into proteolysis-independent regulation by ubiquitin and to define some of the characteristics of this regulatory Ubiquitination pathway. ..
  53. A Novel Proteomics Technology for Protein Farnesylation
    Yingming Zhao; Fiscal Year: 2008
    ..These studies will provide fundamental information for the understanding of molecular mechanisms of FTI functions and are likely to identify novel targets for antitumor drug design. ..
  54. Light activated bioactive substrate release
    RICHARD GIVENS; Fiscal Year: 2008
    ..abstract_text> ..
  55. Bioreactor for Engineered Bioartificial Tissues (BATS)
    Albert Banes; Fiscal Year: 2005
    ..Expansion to a larger bioreactor chamber will allow biomedical researchers to develop better strategies for growth and testing of scaled up tissues for use in vivo in numerous connective tissue applications. ..
  56. Proteome-wide analysis of sumoylation
    Peter Kaiser; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  57. Identification of Genetic Modifiers of an Epstein-Barr Virus Protein.
    AMY ADAMSON; Fiscal Year: 2007
    ..Our studies aim to find human genes whose protein products enhance or suppress Epstein-Barr virus protein activity. [unreadable] [unreadable] [unreadable]..
  58. Cellular Regulation of Papillomavirus E1 Function
    VAN WILSON; Fiscal Year: 2007
    ..abstract_text> ..