Genomes and Genes
Gene Symbol: SPOP
Description: speckle type BTB/POZ protein
Alias: BTBD32, TEF2, speckle-type POZ protein, HIB homolog 1, roadkill homolog 1
- Mutational and expressional analyses of SPOP, a candidate tumor suppressor gene, in prostate, gastric and colorectal cancersMin S Kim
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea
APMIS 121:626-33. 2013..Speckle-type POZ protein (SPOP) is a key adaptor for Cul3-based ubiquitination process...
- Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activityYin Yin Choo
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 117597 Singapore
Mol Biol Cell 22:4706-15. 2011..CSN also did not affect recruitment of the substrate-receptor SPOP to Cul3, suggesting it may not function to facilitate the exchange of Cul3 substrate receptors...
- Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligaseInmaculada Hernández-Muñoz
Division of Molecular Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands
Proc Natl Acad Sci U S A 102:7635-40. 2005..Here, we report that the E3 ubiquitin ligase consisting of SPOP and CULLIN3 is able to ubiquitinate the Polycomb group protein BMI1 and the variant histone MACROH2A...
- Tumor-suppressor role for the SPOP ubiquitin ligase in signal-dependent proteolysis of the oncogenic co-activator SRC-3/AIB1C Li
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA
Oncogene 30:4350-64. 2011..Here, we report that speckle-type POZ protein (SPOP), a cullin 3 (CUL3)-based ubiquitin ligase, is responsible for SRC-3 ubiquitination and proteolysis...
- Prostate cancer-associated mutations in speckle-type POZ protein (SPOP) regulate steroid receptor coactivator 3 protein turnoverChuandong Geng
Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 110:6997-7002. 2013..demonstrated that the E3 ubiquitin ligase adaptor speckle-type poxvirus and zinc finger (POZ) domain protein (SPOP) interacts directly with SRC-3 and promotes its cullin 3-dependent ubiquitination and proteolysis in breast cancer, ..
- Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligasesMin Zhuang
Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Mol Cell 36:39-50. 2009..Here, we present biochemical and structural analyses of the MATH-BTB protein, SPOP. We define a SPOP-binding consensus (SBC) and determine structures revealing recognition of SBCs from the ..
- A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factorQing Zhang
Center for Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
Dev Cell 10:719-29. 2006..The mammalian homolog of HIB, SPOP, can functionally substitute for HIB, and Gli proteins are degraded by HIB/SPOP in Drosophila...
- Identification of a novel nuclear speckle-type protein, SPOPY Nagai
Department of Endocrinology, Medical Research Institute, Tokyo Medical and Dental University, Japan
FEBS Lett 418:23-6. 1997..No zinc finger or RNA recognition motifs were found in this clone. The 2 kbp mRNA encoding the novel clone SPOP (speckle-type POZ protein) was found to be expressed in all human tissues examined...
- Coordinated activation of the nuclear ubiquitin ligase Cul3-SPOP by the generation of phosphatidylinositol 5-phosphateMatthew W Bunce
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
J Biol Chem 283:8678-86. 2008..Using a yeast two-hybrid screen, we identified an interaction between the type IIbeta PIPK isoform (PIPKIIbeta) and SPOP (speckle-type POZ domain protein), a nuclear speckle-associated protein that recruits substrates to Cul3-based ..
- Analysis of Drosophila segmentation network identifies a JNK pathway factor overexpressed in kidney cancerJiang Liu
Institute for Genomics and Systems Biology, University of Chicago and Argonne National Laboratory, Chicago, IL 60637, USA
Science 323:1218-22. 2009..Analysis of the model allowed the identification of a new role for the ubiquitin E3 ligase complex factor SPOP. In Drosophila, the gene encoding SPOP is a target of segmentation transcription factors...
- BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligaseJeong Eun Kwon
NRL of Protein Biochemistry, School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
J Biol Chem 281:12664-72. 2006..b>SPOP is a BTB (Bric-a-brac/Tramtrack/Broad complex) protein that constitutes Cul3-based ubiquitin ligases...
- Speckle-type POZ protein mutations interrupt tumor suppressor function of speckle-type POZ protein in prostate cancer by affecting androgen receptor degradationJohn Lai
Cancer Program, Institute of Health and Biomedical Innovation Australian Prostate Cancer Research Centre Queensland, Translational Research Institute, Queensland University of Technology, Brisbane, Queensland, Australia
Asian J Androl 16:659-60. 2014..Two of these genes include the androgen receptor (AR) and speckle-type POZ protein (SPOP) genes...
- SPOP suppresses tumorigenesis by regulating Hedgehog/Gli2 signaling pathway in gastric cancerChunyan Zeng
J Exp Clin Cancer Res 33:75. 2014..Speckle-type POZ protein, SPOP, is an E3 ubiquitin ligase adaptor, and it is found to inhibit oncogenic signaling...
- Differential regulation of Gli proteins by Sufu in the lung affects PDGF signaling and myofibroblast developmentChuwen Lin
Cardiovascular Research Institute, University of California, San Francisco, CA 94158, United States
Dev Biol 392:324-33. 2014..Otherwise, Numb/Itch would induce Gli1 protein degradation. This is in contrast to inhibition of Spop-mediated degradation of Gli2/3 by Sufu...
- Genomic analyses of gynaecologic carcinosarcomas reveal frequent mutations in chromatin remodelling genesSian Jones
Personal Genome Diagnostics, Baltimore, Maryland 21224, USA
Nat Commun 5:5006. 2014..chromatin remodelling genes, ARID1A and ARID1B, in histone methyltransferase MLL3, in histone deacetylase modifier SPOP and in chromatin assembly factor BAZ1A, in nearly two thirds of cases...
- Androgen receptor is the key transcriptional mediator of the tumor suppressor SPOP in prostate cancerChuandong Geng
Department of Medicine, Baylor College of Medicine, Houston, Texas Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas
Cancer Res 74:5631-43. 2014Somatic missense mutations in the substrate-binding pocket of the E3 ubiquitin ligase adaptor SPOP are present in up to 15% of human prostate adenocarcinomas, but are rare in other malignancies, suggesting a prostate-specific mechanism of ..
- Hedgehog signaling downregulates suppressor of fused through the HIB/SPOP-Crn axis in DrosophilaChen Liu
MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Rd, Pukou District, Nanjing, Jiangsu 210061, China
Cell Res 24:595-609. 2014..Finally, we show that SPOP, the mammalian homologue of HIB, can substitute HIB to downregulate Su(fu) level in Drosophila...
- Decreased expression of the SPOP gene is associated with poor prognosis in gliomaDacheng Ding
Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P R China
Int J Oncol 46:333-41. 2015This study suggests that speckle-type POZ protein (SPOP) may be a tumor suppressor gene and its prognostic value in human glioma...
- Molecular foundations for personalized therapy in prostate cancerKurt W Fisher
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 350 West 11th Street, IUHPL Room 4010, Indianapolis, IN 46202, USA
Curr Drug Targets 16:103-14. 2015..Lastly, we briefly discuss emerging genetic subtypes defined by either SPINK1 overexpression, CHD1 inactivation, or SPOP mutations...
- Yeast translation elongation factor-1A binds vacuole-localized Rho1p to facilitate membrane integrity through F-actin remodelingJames A R Bodman
From the Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 290:4705-16. 2015..Here, we identified eEF1A as a vacuolar Rho1p-interacting protein. eEF1A (encoded by the TEF1 and TEF2 genes in yeast) is an aminoacyl-tRNA transferase needed during protein translation...
- Speckle-type POZ protein, SPOP, is involved in the DNA damage responseDan Zhang
Department of Oncology, The First Affiliated Hospital of Xi an Jiaotong University Medical College, Xi an, Shaanxi 710061, China, Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX 77030, USA
Carcinogenesis 35:1691-7. 2014Speckle-type POZ protein (SPOP) is an adaptor of the cullin 3-based ubiquitin ligase responsible for the degradation of oncoproteins frequently overexpressed in many tumor cells...
- amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiaeDaniel Solis-Escalante
Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
FEMS Yeast Res 13:126-39. 2013..In the present work, the new recyclable dominant marker cassette amdSYM, formed by the Ashbya gossypii TEF2 promoter and terminator and a codon-optimized acetamidase gene (Aspergillus nidulans amdS), is presented...
- Tumor suppressor SPOP ubiquitinates and degrades EglN2 to compromise growth of prostate cancer cellsLinli Zhang
Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, PR China Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Cancer Lett . 2017..Mechanically, Cullin 3-based E3 ubiquitin ligase SPOP, a well-characterized tumor suppressor in prostate cancer, could recognize and destruct EglN2...
- Endometrial cancer-associated mutants of SPOP are defective in regulating estrogen receptor-α protein turnoverP Zhang
1 State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China 2 Shanghai Cancer Center, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
Cell Death Dis 6:e1687. 2015..Speckle-type POZ protein (SPOP) is an adapter protein of the CUL3-based E3 ubiquitin ligase complexes...
- SPOP promotes SIRT2 degradation and suppresses non-small cell lung cancer cell growthJie Luo
Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai 200433, China
Biochem Biophys Res Commun . 2017SIRT2 is a NAD-dependent deacetylase and inhibition of SIRT2 has a broad anticancer activity. Here we report that SPOP binds to SIRT2 and mediates its degradation by the 26S proteasome, which can be blocked by MG132 treatment...
- Prostate cancer-associated mutation in SPOP impairs its ability to target Cdc20 for poly-ubiquitination and degradationFei Wu
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, PR China Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Cancer Lett 385:207-214. 2017Recent studies revealed that mutations in SPOP (Speckle-type POZ protein) occur in up to 15% of patients with prostate cancer. However, the physiological role of SPOP in regulating prostate tumorigenesis remains elusive...
- Mutational burdens and evolutionary ages of thyroid follicular adenoma are comparable to those of follicular carcinomaSeung Hyun Jung
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Korea
Oncotarget 7:69638-69648. 2016..In addition, we found eleven potential cancer-related genes with mutations (EZH1, SPOP, NF1, TCF12, IGF2BP3, KMT2C, CNOT1, BRIP1, KDM5C, STAG2 and MAP4K3) that have not been reported in thyroid ..
- Small-Molecule Targeting of E3 Ligase Adaptor SPOP in Kidney CancerZhong Qiang Guo
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China Department of Urology, Peking University First Hospital, Beijing 100034, China Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China
Cancer Cell 30:474-84. 2016In the cytoplasm of virtually all clear-cell renal cell carcinoma (ccRCC), speckle-type POZ protein (SPOP) is overexpressed and misallocated, which may induce proliferation and promote kidney tumorigenesis...
- Clustering of pelvic floor disorders 20 years after one vaginal or one cesarean birthMaria Gyhagen
Department of Obstetrics and Gynecology, Sahlgrenska Academy at Gothenburg University, Gothenburg, Sweden
Int Urogynecol J 26:1115-21. 2015..prevalence and risk factors for co-occurring pelvic floor disorders (PFDs): urinary incontinence (UI), symptomatic pelvic organ prolapse (sPOP), and fecal incontinence (FI), 20 years after one vaginal (VD) or one cesarean (CS) delivery.
- SPOP promotes tumor progression via activation of β-catenin/TCF4 complex in clear cell renal cell carcinomaWencai Zhao
Department of Urology, Shaanxi Provincal People s Hospital, Xi an, Shaanxi 710068, P R China
Int J Oncol 49:1001-8. 2016..The molecular events supporting RCC progression remain poorly understood. SPOP, an E3 ubiquitin ligase component, was recently showed to sufficiently promote RCC tumorigenesis, however, other ..
- Comprehensive Analysis of the Transcriptional and Mutational Landscape of Follicular and Papillary Thyroid CancersSeong Keun Yoo
Genomic Medicine Institute, Medical Research Center, Seoul National University, Seoul, Republic of Korea
PLoS Genet 12:e1006239. 2016..Mutations in H/K/NRAS, DICER1, EIF1AX, IDH1, PTEN, SOS1, and SPOP were identified in miFTC or FA...
- Speckle-type POZ (pox virus and zinc finger protein) protein gene deletion in ovarian cancer: Fluorescence in situ hybridization analysis of a tissue microarrayXiaoyu Hu
Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P R China
Oncol Lett 12:658-662. 2016..of the present study was to investigate the status of speckle-type POZ (pox virus and zinc finger protein) protein (SPOP) gene located on chromosome 17q21 in ovarian cancer (OC)...
- TRIM24 Is an Oncogenic Transcriptional Activator in Prostate CancerAnna C Groner
Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA Center for Functional Cancer Epigenetics, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Cancer Cell 29:846-58. 2016..We show that recurrent PC-driver mutations in speckle-type POZ protein (SPOP) stabilize the TRIM24 protein, which promotes proliferation under low androgen conditions...
- Long-term effects of vacuum extraction on pelvic floor function: a cohort study in primiparaIda Nilsson
Department of Obstetrics and Gynecology, Sahlgrenska Academy at Gothenburg University, Gothenburg, Sweden
Int Urogynecol J 27:1051-6. 2016We describe the prevalence of symptomatic pelvic organ prolapse (sPOP), urinary incontinence (UI), fecal incontinence (FI) and obstetric anal sphincter injury (OASI) 20 years after one vacuum extraction (VE) delivery compared with one ..
- Identification and Validation of Novel Hedgehog-Responsive Enhancers Predicted by Computational Analysis of Ci/Gli Binding Site DensityKatherine Gurdziel
Department of Cell and Developmental Biology, The University of Michigan, Ann Arbor, MI 48109, United States of America
PLoS ONE 10:e0145225. 2015..factor expressed at the anterior/posterior wing disc boundary; roadkill (rdx), the fly homolog of vertebrate Spop; the segment polarity gene gooseberry (gsb); and two previously untested regions of the Hh receptor-encoding ..
- Relationship between SPOP mutation and breast cancer in Chinese populationM A Khan
Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou, Sichuan, China
Genet Mol Res 14:12362-6. 2015b>SPOP protein has been found to have ubiquitin ligase activity. Mutations in SPOP gene have been recently reported in some cancers such as prostate, gastric, colorectal cancer...
- SPOP Mutations or ERG Rearrangements Result in Enhanced Levels of ERG to Promote Cell Invasion in Prostate CancerShanshan Duan
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA Perlmutter NYU Cancer Center, New York University School of Medicine, New York, NY 10016, USA Howard Hughes Medical Institute, 522 First Avenue, SRB 1107, New York, NY 10016, USA Electronic address
Mol Cell 59:883-4. 2015In this issue, An et al. (2015) and Gan et al. (2015) reveal that the CRL3(SPOP) ubiquitin ligase mediates the degradation of the transcription factor ERG and that translocations of ERG or mutations in SPOP prevent CRL3(SPOP)-dependent ..
- Truncated ERG Oncoproteins from TMPRSS2-ERG Fusions Are Resistant to SPOP-Mediated Proteasome DegradationJian An
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA Department of Urology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA Mayo Clinic Cancer Center, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Mol Cell 59:904-16. 2015b>SPOP mutations and TMPRSS2-ERG rearrangements occur collectively in up to 65% of human prostate cancers. Although the two events are mutually exclusive, it is unclear whether they are functionally interrelated...
- Structural Insights into KCTD Protein Assembly and Cullin3 RecognitionAlan X Ji
Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, ON, M5S 1A8, Canada
J Mol Biol 428:92-107. 2016..Previous crystal structures of the homodimeric BTB proteins KLHL3, KLHL11 and SPOP in complex with the N-terminal domain of Cul3 revealed the features required for Cul3 recognition in these proteins...
- Association between pelvic floor muscle trauma and pelvic organ prolapse 20 years after deliveryIngrid Volløyhaug
Department of Laboratory Medicine, Children s and Women s Health, Norwegian University of Science and Technology, P O Box 8905, Medisinsk teknisk forskningssenter, 7491, Trondheim, Norway
Int Urogynecol J 27:39-45. 2016..pelvic floor muscle trauma (PFMT) after vaginal delivery is associated with pelvic organ prolapse (POP) symptoms (sPOP) and signs (POP-Q ≥2) in patient populations...
- The ubiquitin conjugating enzyme, UbcM2, engages in novel interactions with components of cullin-3 based E3 ligasesKendra S Plafker
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
Biochemistry 48:3527-37. 2009..Together, these studies represent the first evidence that UbcM2, in concert with substrate adaptors, engages activated CUL3 ligases, thus suggesting that class III E2s are novel regulators of cullin ligases...
- In vivo topography of Rap1p-DNA complex at Saccharomyces cerevisiae TEF2 UAS(RPG) during transcriptional regulationVeronica De Sanctis
Fondazione Istituto Pasteur Fondazione Cenci Bolognetti, c o Dipartimento di Genetica e Biologia Molecolare, Universita di Roma, La Sapienza, Rome, Italy
J Mol Biol 318:333-49. 2002..The implications of these results for the mechanism of Rap1p action are discussed...
- Exome sequencing of serous endometrial tumors identifies recurrent somatic mutations in chromatin-remodeling and ubiquitin ligase complex genesMatthieu Le Gallo
Cancer Genetics Branch, National Human Genome Research Institute, US National Institutes of Health NIH, Bethesda, MD, USA
Nat Genet 44:1310-5. 2012..identified high frequencies of somatic mutations in CHD4 (17%), EP300 (8%), ARID1A (6%), TSPYL2 (6%), FBXW7 (29%), SPOP (8%), MAP3K4 (6%) and ABCC9 (6%). Overall, 36...
- TFE2 and GATA3 enhance induction of POU4F3 and myosin VIIa positive cells in nonsensory cochlear epithelium by ATOH1Masatsugu Masuda
Departments of Surgery Otolaryngology, La Jolla, CA 92093 0666, USA
Dev Biol 372:68-80. 2012..of the greater epithelial ridge with multiple plasmids encoding human ATOH1 (hATOH1), hTCF3 (also known as E2A or TEF2), hGATA3, and hSP1. hATOH1 or hTCF3 alone induced Pou4f3/GFP cells but hGATA3 and hSP1 did not...
- Insight in glioma susceptibility through an analysis of 6p22.3, 12p13.33-12.1, 17q22-23.2 and 18q23 SNP genotypes in familial and non-familial gliomaYanhong Liu
Dan L Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA
Hum Genet 131:1507-17. 2012..33-12.1 PRMT8 rs17780102 (P = 0.031) and 17q12-21.32 SPOP rs650461 (P = 0.025)...
- Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCRYuanyuan Xu
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
Biochem Biophys Res Commun 424:398-403. 2012..RNA polymerase-II transcription factor (RPII), Elongation factor 1-b (EF-1b) and Translation elongation factor 2 (TEF2)...
- FTH1 binds to Daxx and inhibits Daxx-mediated cell apoptosisFang Liu
Department of Clinical Laboratory, the First People s Hospital in Jiangbei District, Chongqing 400020, China
Mol Biol Rep 39:873-9. 2012..We present evidence to demonstrate the FTH1 and Daxx are able to participate in apoptosis pathway through JNK signal molecule and FTH1 can inhibit this pathway...
- Mechanism of cullin3 E3 ubiquitin ligase dimerizationYin Yin Choo
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
PLoS ONE 7:e41350. 2012..Our results provide evidence for BTB substrate receptor dependent Cul3 dimerization which is likely to play an important role in promoting substrate ubiquitination...
- Prevalence and risk factors for pelvic organ prolapse 20 years after childbirth: a national cohort study in singleton primiparae after vaginal or caesarean deliveryM Gyhagen
Department of Obstetrics and Gynaecology, Sahlgrenska Academy at Gothenburg University, Gothenburg, Sweden
BJOG 120:152-60. 2013To investigate prevalence and risk factors for symptomatic pelvic organ prolapse (sPOP) and sPOP concomitant with urinary incontinence (UI) in women 20 years after one vaginal delivery or one caesarean delivery.
- Structural basis of high-order oligomerization of the cullin-3 adaptor SPOPLaura K van Geersdaele
School of Biology, University of Leeds, Leeds LS2 9JT, England
Acta Crystallogr D Biol Crystallogr 69:1677-84. 2013..b>SPOP is a BTB-domain adaptor of the cullin-3 E3 ligase complexes; it selectively recruits substrates via its N-terminal ..
- Spinal repositioning deficit. The effect of prolonged flexed postureVasileios Korakakis
Department of Physical Education and Sport Science, University of Thessaly, Trikala, Greece
Br J Sports Med 47:e3. 2013..active subjects were assessed regarding their habitual sitting posture (HSP), perception of optimal posture (SPOP), ability to adopt an instructed sitting posture (IOSP) and accuracy to reproduce the criterion posture immediately,..
- Stabilization of speckle-type POZ protein (Spop) by Daz interacting protein 1 (Dzip1) is essential for Gli turnover and the proper output of Hedgehog signalingTyler Schwend
From the Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois at Urbana Champaign, Urbana, Illinois 61802
J Biol Chem 288:32809-20. 2013..Here, we report that Dzip1 regulates Gli/Ci turnover by preventing degradation of speckle-type POZ protein (Spop), a protein that promotes proteasome-dependent turnover of Gli proteins...
- Molecular pathology and prostate cancer therapeutics: from biology to bedsideDaniel Nava Rodrigues
Prostate Cancer Targeted Therapy Group and Drug Development Unit, Royal Marsden NHS Foundation Trust and Institute of Cancer Research, Sutton, Surrey, UK
J Pathol 232:178-84. 2014..Tumours without this rearrangement include SPOP mutant as well as SPINK1-over-expressing subtypes...
- Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiaeDaniel Shenton
Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology UMIST, P O Box 88, Manchester M60 1QD, UK
Biochem J 374:513-9. 2003..a range of glycolytic and related enzymes (Tdh3, Eno2, Adh1, Tpi1, Ald6 and Fba1), as well as translation factors (Tef2, Tef5, Nip1 and Rps5) are identified...
- [Construction and preliminary applications of a Saccharomyces cerevisiae detection plasmid using for screening promoter elements]Zhi fang Wang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Yao Xue Xue Bao 48:228-35. 2013..The fluorescence microscopy results indicated that the six promoters (GAL1, GAL2, GAL7, GAL10, TEF2 and PGK1) can regulate the expression of DsRed-Monomer gene...
- Single cell analysis of yeast replicative aging using a new generation of microfluidic deviceYi Zhang
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, Center for Quantitative Biology, and School of Physics, Peking University, China
PLoS ONE 7:e48275. 2012..throughout their lifespan, we discovered a surprising change of expression of the translation elongation factor TEF2 during aging, suggesting altered translational control in aged mother cells...
- Exome sequencing identifies recurrent SPOP, FOXA1 and MED12 mutations in prostate cancerChristopher E Barbieri
Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA
Nat Genet 44:685-9. 2012..New recurrent mutations were identified in multiple genes, including MED12 and FOXA1. SPOP was the most frequently mutated gene, with mutations involving the SPOP substrate-binding cleft in 6-15% of tumors ..
- Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligaseWesley J Errington
Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario M5S 1A8, Canada
Structure 20:1141-53. 2012..Here, we describe the dimeric structure of the E3 ligase adaptor protein SPOP (speckle-type POZ protein) in complex with the N-terminal domain of Cul3 at 2.4 Å resolution...
- A single heterochromatin boundary element imposes position-independent antisilencing activity in Saccharomyces cerevisiae minichromosomesSangita A Chakraborty
Department of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Milton S Hershey Medical Center, Hershey, Pennsylvania, United States of America
PLoS ONE 6:e24835. 2011..multi-copy yeast minichromosome and we further showed that two distinct heterochromatin boundary elements STAR and TEF2-UASrpg are able to limit the heterochromatin spreading in circular minichromosomes...
- Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiaeS U Aström
Department of Microbiology, Umea University, Sweden
Mol Gen Genet 261:967-76. 1999..On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1alpha (eEF-1alpha), into rit1 null strains with two intact ..
- Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOPMuhnho La
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305 701, Republic of Korea
Biochem Biophys Res Commun 320:760-5. 2004Daxx-mediated transcriptional repression was modulated by a speckled POZ domain protein SPOP which was first identified as an autoantigen from the serum of a scleroderma patient...
- Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activityAihua Liu
Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, and Penn Diabetes Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Mol Cell Biol 24:4372-83. 2004..We have identified a novel POZ domain protein, PDX-1 C terminus-interacting factor 1 (PCIF1)/SPOP, that interacts with PDX-1 both in vitro and in vivo...
- NKG2D-DAP10 triggers human NK cell-mediated killing via a Syk-independent regulatory pathwayDaniel D Billadeau
Division of Oncology Research, Mayo Graduate and Medical Schools, Mayo Clinic, Rochester, Minnesota 55905, USA
Nat Immunol 4:557-64. 2003..Yet the signals initiated by NKG2D-DAP10 are fully capable of inducing killing. Our findings identify a previously unknown mechanism by which receptor complexes that lack ITAM motifs can trigger lymphocyte activation...
- Selective associations with signaling proteins determine stimulatory versus costimulatory activity of NKG2DAndreas Diefenbach
Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720 3200, USA
Nat Immunol 3:1142-9. 2002..This strategy suggests a rationale for the multisubunit structure of stimulatory immunoreceptors...
- NKG2D recruits two distinct adapters to trigger NK cell activation and costimulationSusan Gilfillan
Department of Pathology and Immunology, Washington University School of Medicine, 660 S Euclid, St Louis, MO 63110, USA
Nat Immunol 3:1150-5. 2002..Thus, NKG2D is a versatile receptor that, depending on the availability of adapter partners, mediates costimulation in T cells and/or activation in NK cells...
- MacroH2A1.2 binds the nuclear protein SpopIchiro Takahashi
Division of Genetic Resources, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
Biochim Biophys Acta 1591:63-8. 2002..2. The results showed that macroH2A1.2 interacts with mouse nuclear speckled type protein Spop. The Spop protein has a unique composition: an N-terminal MATH, and a C-terminal BTB/POZ domain...
- A diverse family of proteins containing tumor necrosis factor receptor-associated factor domainsJ M Zapata
Burnham Institute, La Jolla, California 92037, USA
J Biol Chem 276:24242-52. 2001..MUL, the product of the causative gene in Mulibrey Nanism syndrome; USP7 (HAUSP), an ubiquitin protease; and SPOP, a POZ domain-containing protein...
- PCR-based gene targeting in the filamentous fungus Ashbya gossypiiJ Wendland
, Biozentrum, University of Basel, Switzerland
Gene 242:381-91. 2000..Escherichia coli kanR gene under the control of promoter and terminator sequences of the Saccharomyces cerevisiae TEF2 gene and allows selection of G418/geneticin-resistant transformants...
- Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptusDavid Kent
Department of Cell and Developmental Biology, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA
Development 133:2001-10. 2006..The existence of human orthologs for Rdx raises the possibility that this novel feedback loop also modulates Hh responses in humans...
- An activating immunoreceptor complex formed by NKG2D and DAP10J Wu
DNAX Research Institute, 901 California Avenue, Palo Alto, CA 94304, USA
Science 285:730-2. 1999..Thus, NKG2D-DAP10 receptor complexes may activate NK and T cell responses against MICA-bearing tumors...
- The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11J I Piruat
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Spain
Genetics 147:1585-94. 1997..Constitutive transcription was not affected, as determined in ADH1 and TEF2. In addition, Hrs1p was required for mating-factor expression, telomere-linked DNA silencing and DNA supercoiling ..
- Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 betaT G Kinzy
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
Genetics 141:481-9. 1995..a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha...
- GCR1, a transcriptional activator in Saccharomyces cerevisiae, complexes with RAP1 and can function without its DNA binding domainJ Tornow
Department of Biological Sciences, University of Southern Mississippi, Hattiesburg 39406 5018
EMBO J 12:2431-7. 1993..GCR1-dependent transcription of either the glycolytic gene ADH1 or the translational component genes TEF1 and TEF2. We have also identified an activation domain within a segment of the GCR1 protein (the N-terminal third) that is ..
- Cloning and genetic characterization of a calcium- and phospholipid-binding protein from Saccharomyces cerevisiae that is homologous to translation elongation factor-1 gammaN G Kambouris
Department of Pharmacology, University of Virginia, Charlottesville 22903
Yeast 9:151-63. 1993..No evidence was found for an interaction of CAM1 with TEF1, TEF2, SEC4, YPT1, RAS1, RAS2, CDC6, ARF1, ARF2 or CIN4...
- Either one of the two yeast EF-1 alpha genes is required for cell viabilityP Cottrelle
Service de Biochimie, C E N SACLAY, Gif sur Yvette, France
Curr Genet 9:693-7. 1985Two genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae...
- Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sitesG M Santangelo
Department of Biology, Portland State University, Oregon 97201 0751
Mol Cell Biol 10:859-62. 1990..We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption...
- Efficient expression of the Saccharomyces cerevisiae glycolytic gene ADH1 is dependent upon a cis-acting regulatory element (UASRPG) found initially in genes encoding ribosomal proteinsJ Tornow
Biology Department, Portland State University, OR 97201 0751
Gene 90:79-85. 1990..In band retardation assays, the promoters for the elongation factor 1 alpha-encoding genes (TEF1 and TEF2) competed for binding of the protein to the copy of UASRPG in the ADH1 promoter...
- Variations in Hedgehog signaling: divergence and perpetuation in Sufu regulation of GliLaurent Ruel
Institut Biologie du Développement et Cancer IBDC, Universite de Nice Sophia Antipolis, CNRS UMR 6543, Centre de Biochimie, Nice Cedex 02, France
Genes Dev 23:1843-8. 2009..1910-1928) demonstrate that mouse Suppressor of fused (Sufu) regulates the stability of the transcription factors Gli2 and Gli3 by antagonizing the conserved Gli degradation device mediated by Hib/Spop in a cilia-independent manner.
- Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiaeM A Huie
Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville 32610 0266
Mol Cell Biol 12:2690-700. 1992..detect specific interactions with CTTCC sequence elements found in front of the translational component genes TEF1, TEF2, and CRY1...
- Pcif1 modulates Pdx1 protein stability and pancreatic β cell function and survival in miceKathryn C Claiborn
Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Clin Invest 120:3713-21. 2010..Pdx1 C terminus-interacting factor-1 (Pcif1, also known as SPOP) is a nuclear protein that inhibits Pdx1 transactivation...
- Suppressor of fused and Spop regulate the stability, processing and function of Gli2 and Gli3 full-length activators but not their repressorsChengbing Wang
Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA
Development 137:2001-9. 2010..By contrast, RNAi knockdown of Spop (a substrate-binding adaptor for the cullin3-based ubiquitin E3 ligase) in Sufu mutant mouse embryonic fibroblasts (..
- Evolutionary expansion of SPOP and associated TD/POZ gene family: impact of evolutionary route on gene expression patternKong Bung Choo
Department of Medical Research and Education, Taipei Veterans General Hospital, Shipai, Taipei, Taiwan
Gene 460:39-47. 2010..and -T1 (rT2 and rT1) retrogenes are members of the TD/POZ gene family which also includes the well-characterized SPOP gene...
- Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradationXiaohui Wen
Genentech, South San Francisco, CA 94080, USA
Mol Cell Biol 30:1910-22. 2010..to accumulation of full-length Gli3, but instead renders it labile, leading to its proteasomal degradation via the SPOP/Cul3 complex...
- Multiple Ser/Thr-rich degrons mediate the degradation of Ci/Gli by the Cul3-HIB/SPOP E3 ubiquitin ligaseQing Zhang
Departments of Developmental Biology and Pharmacology, University of Texas Southwestern Medical Center at Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 106:21191-6. 2009..factor cubitus interruptus (Ci)/Gli by Cul3-Hedghog-induced MATH and BTB domain-containing protein (HIB)/SPOP. Ci uses multiple Ser/Thr (S/T)-rich motifs that bind HIB cooperatively to mediate its degradation...
- Functional screening of FxxLF-like peptide motifs identifies SMARCD1/BAF60a as an androgen receptor cofactor that modulates TMPRSS2 expressionDennis J van de Wijngaart
Department of Urology, Josephine Nefkens Institute, Erasmus Medical College, P O Box 2040, 3000 CA Rotterdam, The Netherlands
Mol Endocrinol 23:1776-86. 2009..In summary, our data demonstrate that BAF60a directly interacts with the coactivator groove in the AR ligand-binding domain via its FxxFF motif, thereby selectively activating specific AR-driven promoters...
- Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conservedMiao Hsueh Chen
Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California 94158, USA
Genes Dev 23:1910-28. 2009..Consistent with this, Sufu regulates Gli protein levels by antagonizing the activity of Spop, a conserved Gli-degrading factor...
- Breast cancer metastasis suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complexBogyou Kim
Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 151 742, South Korea
Biochem Biophys Res Commun 415:720-6. 2011..ubiquitin ligase, is a new binding partner of BRMS1 and the interaction between BRMS1 and Cul3 is mediated by the SPOP adaptor protein...
- Selection of reliable reference genes for gene expression studies in peach using real-time PCRZhaoguo Tong
College of Horticulture, Nanjing Agricultural University, 1 Tongwei Road, Weigang, Nanjing 210095, PR China
BMC Mol Biol 10:71. 2009..Only a few studies on reference genes have been done in plants and none in peach (Prunus persica L. Batsch). Therefore, the present study was conducted to identify suitable reference gene(s) for normalization of gene expression in peach...
- Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeastIgor A Valouev
Cardiology Research Center, 121552 Moscow, Russia
BMC Mol Biol 10:60. 2009..Besides termination, both release factors have essential, but poorly characterized functions outside of translation...
- The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cellsCristina M Cummings
Department of Molecular Biology, Cell Biology and Biochemistry and the Center for Genomics and Proteomics, Brown University, Providence, Rhode Island 02903, USA
J Biol Chem 284:11663-75. 2009..We demonstrate a novel regulatory mechanism for p60/katanin that occurs at the level of targeted proteolysis to allow normal mitotic progression in mammalian cells...
- BTB/POZ domain of speckle-type POZ protein (SPOP) confers proapoptotic function in HeLa cellsBoohyeong Byun
Department of Oriental Medicine, Daegu Haany University, Daegu, South Korea
Biofactors 31:165-9. 2007The proapoptotic function of SPOP protein was investigated in HeLa cells. HeLa cells underwent apoptosis by the overexpression of SPOP...
- Repression of transcriptional activity of estrogen receptor alpha by a Cullin3/SPOP ubiquitin E3 ligase complexBoohyeong Byun
Department of Oriental Medicine, Daegu Haany University, Daegu 705 060, Korea
Mol Cells 25:289-93. 2008The role of SPOP in the ubiquitination of ER alpha by the Cullin3-based E3 ubiquitin ligase complex was investigated...
- Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase IIKoji Kasahara
Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, 230 0045, Japan
Nucleic Acids Res 36:1343-57. 2008..upstream shift in transcriptional start sites of RPS5, RPS16A, RPL23B, RPL27B and RPL32, but not of RPS31, RPL10, TEF2 and ADH1, indicating that HMO1 may participate in start site selection of a subset of class II genes presumably via ..
- Overproduction of translation elongation factor 1-alpha (eEF1A) suppresses the peroxisome biogenesis defect in a Hansenula polymorpha pex3 mutant via translational read-throughJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, The Netherlands
FEMS Yeast Res 7:1114-25. 2007..We have isolated two genes (TEF1 and TEF2) that encode eEF1A, and which are constitutively expressed...
- Multiple ubiquitin-dependent processing pathways regulate hedgehog/gli signaling: implications for cell development and tumorigenesisLucia Di Marcotullio
Department of Experimental Medicine and Pathology, Cenci Bolognetti Foundation, University La Sapienza, Roma, Italy
Cell Cycle 6:390-3. 2007..Cullin family-based ubiquitination results in either Cullin1-Slimb/betaTrCP- or Cullin3-HIB/Roadkill/SPOP-dependent proteolytic processing or degradation of Drosophila Cubitus interruptus or mammalian Gli proteins...
- The role of Pcif1 in pancreas developmentJEFFREY CHARLES RAUM; Fiscal Year: 2012..Recently, the MATH-BTB protein, Pcif1 (or SPOP) has been identified as a Pdx-1 interacting protein and subsequently shown to recruit Pdx-1 for ubiquitin-mediated ..
- Mark A Rubin; Fiscal Year: 2016..The SPOP gene (Speckle-type POZ Protein) encodes for the substrate-recognition component of a Cullin3- based E3-ubiquitin ..