SPINT2

Summary

Gene Symbol: SPINT2
Description: serine peptidase inhibitor, Kunitz type 2
Alias: DIAR3, HAI-2, HAI2, Kop, kunitz-type protease inhibitor 2, hepatocyte growth factor activator inhibitor type 2, serine protease inhibitor, Kunitz type, 2, testicular tissue protein Li 183
Species: human

Top Publications

  1. ncbi Cryocrystallography of a Kunitz-type serine protease inhibitor: the 90 K structure of winged bean chymotrypsin inhibitor (WCI) at 2.13 A resolution
    S Ravichandran
    Crystallography Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Calcutta 700 064, India
    Acta Crystallogr D Biol Crystallogr 55:1814-21. 1999
  2. ncbi Bikunin suppresses lipopolysaccharide-induced lethality through down-regulation of tumor necrosis factor- alpha and interleukin-1 beta in macrophages
    Kiyoshi Wakahara
    NetForce Co, Ltd, Aichi, Japan
    J Infect Dis 191:930-8. 2005
  3. doi HAI-2 is epigenetically downregulated in human hepatocellular carcinoma, and its Kunitz domain type 1 is critical for anti-invasive functions
    Edmund Kwok Kwan Tung
    Department of Pathology, Faculty of Medicine, Liver Cancer and Hepatitis Research Laboratory and S H Ho Foundation Research Laboratories, The University of Hong Kong, Pokfulam, Hong Kong
    Int J Cancer 124:1811-9. 2009
  4. ncbi Hepatocyte growth factor activator inhibitor, a novel Kunitz-type serine protease inhibitor
    T Shimomura
    Research Center, Mitsubishi Chemical Corp, Aoba ku, Yokohama 227, Japan
    J Biol Chem 272:6370-6. 1997
  5. ncbi Identification and cloning of human placental bikunin, a novel serine protease inhibitor containing two Kunitz domains
    C W Marlor
    Institute of Bone and Joint Disease and Cancer, Bayer Corporation, West Haven, Connecticut 06516, USA
    J Biol Chem 272:12202-8. 1997
  6. ncbi Purification and cloning of hepatocyte growth factor activator inhibitor type 2, a Kunitz-type serine protease inhibitor
    T Kawaguchi
    Research Center, Mitsubishi Chemical Corp, Aoba ku, Yokohama 227, Japan
    J Biol Chem 272:27558-64. 1997
  7. ncbi Cloning of a new Kunitz-type protease inhibitor with a putative transmembrane domain overexpressed in pancreatic cancer
    F Müller-Pillasch
    Department of Internal Medicine I, University of Ulm, Germany
    Biochim Biophys Acta 1395:88-95. 1998
  8. pmc Potent inhibition and global co-localization implicate the transmembrane Kunitz-type serine protease inhibitor hepatocyte growth factor activator inhibitor-2 in the regulation of epithelial matriptase activity
    Roman Szabo
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:29495-504. 2008
  9. pmc Mutations in SPINT2 cause a syndromic form of congenital sodium diarrhea
    Peter Heinz-Erian
    Department of Pediatrics II, Innsbruck Medical University, A 6020 Innsbruck, Austria
    Am J Hum Genet 84:188-96. 2009
  10. pmc Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice
    Roman Szabo
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Development 136:2653-63. 2009

Research Grants

  1. THOMAS EDWARD PRISINZANO; Fiscal Year: 2016
  2. Yong Zhang; Fiscal Year: 2014
  3. Developing a ClO2 Compliance Service: Minimizing Water Borne Microbial Infection
    SPENDEL U WOLFGANG; Fiscal Year: 2011
  4. NOVEL SERINE PROTEASE IN INVASIVE CARCINOMAS
    Robert Dickson; Fiscal Year: 2000
  5. Targeting Opioid Receptor Heterodimers for Pain Treatment
    Selena Bartlett; Fiscal Year: 2007
  6. 'Latinos' Health Literacy, Social Support, and OH-KOP"
    Maria Rosa Watson; Fiscal Year: 2006
  7. MOLECULAR REGULATION OF ARTERIAL THROMBOSIS
    Robert Simari; Fiscal Year: 2009
  8. Controlling Apoptosis in Oligondendrocytes
    Pamela Knapp; Fiscal Year: 2005
  9. NOVEL PHARMACOTHERAPY FOR TREATMENT OF COCAINE ADDICTION
    Frank Carroll; Fiscal Year: 2008
  10. Design and Development of Pharmacotherapies for Treating Stimulant Abuse
    Frank Ivy Carroll; Fiscal Year: 2010

Patents

  1. Human cDNAs and proteins and uses thereof

Detail Information

Publications195 found, 100 shown here

  1. ncbi Cryocrystallography of a Kunitz-type serine protease inhibitor: the 90 K structure of winged bean chymotrypsin inhibitor (WCI) at 2.13 A resolution
    S Ravichandran
    Crystallography Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Calcutta 700 064, India
    Acta Crystallogr D Biol Crystallogr 55:1814-21. 1999
    ..A total of 176 ordered water molecules and five sulfate ions are included in the model...
  2. ncbi Bikunin suppresses lipopolysaccharide-induced lethality through down-regulation of tumor necrosis factor- alpha and interleukin-1 beta in macrophages
    Kiyoshi Wakahara
    NetForce Co, Ltd, Aichi, Japan
    J Infect Dis 191:930-8. 2005
    ..Lipopolysaccharide (LPS) is the primary mediator of gram-negative sepsis; it induces the production of macrophage-derived cytokines. It has been shown that bikunin, a Kunitz-type protease inhibitor, inhibits LPS-induced cytokine expression...
  3. doi HAI-2 is epigenetically downregulated in human hepatocellular carcinoma, and its Kunitz domain type 1 is critical for anti-invasive functions
    Edmund Kwok Kwan Tung
    Department of Pathology, Faculty of Medicine, Liver Cancer and Hepatitis Research Laboratory and S H Ho Foundation Research Laboratories, The University of Hong Kong, Pokfulam, Hong Kong
    Int J Cancer 124:1811-9. 2009
    ..Our findings suggest that HAI-2 is a candidate tumour suppressor gene that is frequently hypermethylated and underexpressed in human HCCs, and the KD-1 domain of HAI-2 is the key region responsible for its anti-invasive function...
  4. ncbi Hepatocyte growth factor activator inhibitor, a novel Kunitz-type serine protease inhibitor
    T Shimomura
    Research Center, Mitsubishi Chemical Corp, Aoba ku, Yokohama 227, Japan
    J Biol Chem 272:6370-6. 1997
    ..These results suggest that the inhibitor may be produced as a membrane-associated form and secreted by the producing cells as a proteolytically truncated form...
  5. ncbi Identification and cloning of human placental bikunin, a novel serine protease inhibitor containing two Kunitz domains
    C W Marlor
    Institute of Bone and Joint Disease and Cancer, Bayer Corporation, West Haven, Connecticut 06516, USA
    J Biol Chem 272:12202-8. 1997
    ..The natural protein exhibited the same NH2 terminus as predicted from the cloned cDNA and inhibited trypsin, plasma kallikrein, and plasmin with IC50 values in the nanomolar range...
  6. ncbi Purification and cloning of hepatocyte growth factor activator inhibitor type 2, a Kunitz-type serine protease inhibitor
    T Kawaguchi
    Research Center, Mitsubishi Chemical Corp, Aoba ku, Yokohama 227, Japan
    J Biol Chem 272:27558-64. 1997
    ..Because HAI-2 and HAI-1 are potent inhibitors specific for HGF activator, they may be involved in regulation of proteolytic activation of HGF in injured tissues...
  7. ncbi Cloning of a new Kunitz-type protease inhibitor with a putative transmembrane domain overexpressed in pancreatic cancer
    F Müller-Pillasch
    Department of Internal Medicine I, University of Ulm, Germany
    Biochim Biophys Acta 1395:88-95. 1998
    ..The identified gene named kop (Kunitz domain containing protein overexpressed in pancreatic cancer) was assigned to chromosome 19 in the region ..
  8. pmc Potent inhibition and global co-localization implicate the transmembrane Kunitz-type serine protease inhibitor hepatocyte growth factor activator inhibitor-2 in the regulation of epithelial matriptase activity
    Roman Szabo
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:29495-504. 2008
    ..Unlike HAI-1 and matriptase, however, HAI-2 expression was also detected in non-epithelial cells of brain and lymph nodes, suggesting that HAI-2 may also be involved in inhibition of serine proteases other than matriptase...
  9. pmc Mutations in SPINT2 cause a syndromic form of congenital sodium diarrhea
    Peter Heinz-Erian
    Department of Pediatrics II, Innsbruck Medical University, A 6020 Innsbruck, Austria
    Am J Hum Genet 84:188-96. 2009
    ..A genome-wide SNP scan was applied and identified a homozygous c.593-1G-->A splicing mutation in SPINT2, encoding a Kunitz-type serine-protease inhibitor, in one extended kindred with syndromic CSD...
  10. pmc Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice
    Roman Szabo
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Development 136:2653-63. 2009
    Hypomorphic mutations in the human SPINT2 gene cause a broad spectrum of abnormalities in organogenesis, including organ and digit duplications, atresia, fistulas, hypertelorism, cleft palate and hamartoma...
  11. doi Genetic characterization of congenital tufting enteropathy: epcam associated phenotype and involvement of SPINT2 in the syndromic form
    Julie Salomon
    Department of Pediatric Gastroenterology, Hepatology and Nutrition, Assistance Publique Hopitaux de Paris, Necker Enfants Malades Hospital, Universite Paris Descartes, Sorbonne Paris Cité, 149 rue de Sevres, 75015, Paris, France
    Hum Genet 133:299-310. 2014
    ..Here we establish SPINT2, previously ascribed to congenital sodium diarrhea, as a second gene associated with CTE and report molecular and ..
  12. doi TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor
    Tomio Hashimoto
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori ku, Yokohama, Japan
    FEBS J 277:4888-900. 2010
    ..These results suggest that TMPRSS13 functions as an HGF-converting protease, the activity of which may be regulated by HAI-1...
  13. ncbi Molecular cloning of a novel multidomain Kunitz-type proteinase inhibitor from the hookworm Ancylostoma caninum
    John M Hawdon
    Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, 725 Ross Hall, 2300 Eye Street NW, Washington, DC 20037, USA
    J Parasitol 89:402-7. 2003
    ..In addition, there are 44 nematode proteins containing one or more Kunitz domains in GenBank, including several with multiple domains...
  14. ncbi Antitussive profile of the NOP agonist Ro-64-6198 in the guinea pig
    Robbie L McLeod
    Allergy, Schering Plough Research Institute, Kenilworth, N J 07033 1300, USA
    Pharmacology 71:143-9. 2004
    ..The Ki values for Ro-64-6198 at NOP, MOP, KOP and DOP receptors was 0.3, 36, 214 and 3,787 nmol/l, respectively...
  15. ncbi Roles of hepatocyte growth factor (HGF) activator and HGF activator inhibitor in the pericellular activation of HGF/scatter factor
    Hiroaki Kataoka
    Second Department of Pathology, Miyazaki Medical College, Miyazaki, Japan
    Cancer Metastasis Rev 22:223-36. 2003
    ....
  16. ncbi Purification and characterization of a complex containing matriptase and a Kunitz-type serine protease inhibitor from human milk
    C Y Lin
    Lombardi Cancer Center, Georgetown University Medical Center, Washington, D C 20007, USA
    J Biol Chem 274:18237-42. 1999
    ....
  17. ncbi Multiple sites of proteolytic cleavage to release soluble forms of hepatocyte growth factor activator inhibitor type 1 from a transmembrane form
    T Shimomura
    Research Center, Mitsubishi Chemical Corp, Kamoshida, Aoba ku, Yokohama, 227 8502, Japan
    J Biochem 126:821-8. 1999
    ..Further, the soluble forms of HAI-1 have different inhibitory activity against HGF activator. These findings suggest that proteolytic processing plays important roles in regulation of the inhibitory activity of HAI-1...
  18. pmc Glial-restricted precursors: patterns of expression of opioid receptors and relationship to human immunodeficiency virus-1 Tat and morphine susceptibility in vitro
    S K Buch
    Department of Anatomy and Neurobiology, University of Kentucky, College of Medicine, 800 Rose Street, Lexington, KY 40536 0298, USA
    Neuroscience 146:1546-54. 2007
    ..At 1 day in vitro (DIV), GRPs failed to express mu opioid receptors (MOR or MOP) or kappa-opioid receptors (KOR or KOP); however, at 5 DIV, most GRPs expressed MOR and KOR...
  19. ncbi Mouse hepatocyte growth factor (HGF) activator inhibitor type 2 lacking the first Kunitz domain potently inhibits the HGF activator
    Hiroaki Kataoka
    Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki, 889 1692, Japan
    Biochem Biophys Res Commun 290:1096-100. 2002
    b>Hepatocyte growth factor activator inhibitor type 2 (HAI-2) is a serine proteinase inhibitor containing two Kunitz-type inhibitor domains, initially identified as a potent inhibitor of hepatocyte growth factor activator (HGFA)...
  20. pmc Cell-specific loss of kappa-opioid receptors in oligodendrocytes of the dysmyelinating jimpy mouse
    Pamela E Knapp
    Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Neurosci Lett 451:114-8. 2009
    ..This paper describes a severe reduction in expression of kappa-opioid receptors (KOP) in oligodendrocytes of jimpy mice. A cell-specific reduction of >90% is apparent by 5 days of age...
  21. ncbi Genomic organization of the human amyloid beta-protein precursor gene
    S Yoshikai
    Research Laboratory for Genetic Information, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Gene 87:257-63. 1990
    ..Northern blotting showed that the transcription of the BPP gene was apparently induced by 12-O-tetradecanoylphorbol-13-acetate (phorbol derivative) in HeLa cells...
  22. ncbi Nociceptin inhibits vanilloid TRPV-1-mediated neurosensitization induced by fenoterol in human isolated bronchi
    Christophe Faisy
    Unité de Recherche de l Enseignement Supérieur, Equipe d Accueil 220, Faculte de Medecine Paris Ile de France Ouest, Unité de Formation et de Recherche Biomédicale des Saint Pères, 45 rue des Saint Peres, 75006, Paris, France
    Naunyn Schmiedebergs Arch Pharmacol 370:167-75. 2004
    ..Tertiapin (10 microM), an inhibitor of the inward-rectifier K(+) channels, but not naloxone (0.1 microM), a DOP/KOP/MOP receptor antagonist, prevented the inhibitory effect of nociceptin...
  23. doi Binding studies of novel, non-mammalian enkephalins, structures predicted from frog and lungfish brain cDNA sequences
    E Bojnik
    Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt 62, 6726 Szeged, Hungary
    Neuroscience 158:867-74. 2009
    ..toward the mu-opioid receptor (MOP) and DOP, while exhibiting moderate affinity toward the kappa-opioid receptor (KOP)...
  24. pmc In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains
    Béatrice Pignolet
    Laboratory Interactions Hôtes Virus et Vaccinologie, UMR 1225 INRA ENVT, Ecole Nationale Veterinaire de Toulouse, 23 Chemin des Capelles, Toulouse F 31076, France
    Virol J 4:94. 2007
    ..We showed that bovine KOP-R, BT and MDBK cell lines do not support MYXV production...
  25. pmc The omptins of Yersinia pestis and Salmonella enterica cleave the reactive center loop of plasminogen activator inhibitor 1
    Johanna Haiko
    Department of Biosciences, PO Box 56, University of Helsinki, FI 00014 Helsinki, Finland
    J Bacteriol 192:4553-61. 2010
    ..Cleavage of PAI-1 was also detected with the omptins Epo of Erwinia pyrifoliae and Kop of Klebsiella pneumoniae, which both belong to the same omptin subfamily as Pla and PgtE, whereas no cleavage of ..
  26. ncbi Hepatocyte growth factor activator inhibitors (HAI-1 and HAI-2) are potential targets in uterine leiomyosarcoma
    Keiichiro Nakamura
    Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, 2 5 1 Shikata cho, Kita ku, Okayama 700 8558, Japan
    Int J Oncol 37:605-14. 2010
    ..These findings indicate that HAI-1 and HAI-2 may be possible tumor suppressor genes for uterine LMS and thus, both could be considered therapeutic agents for the treatment of LMS...
  27. doi Attitudes toward the elderly with CNS trauma: a cross-sectional study of neuroscientists, clinicians, and allied-health professionals
    Julio C Furlan
    Division of Genetics and Development, Toronto Western Research Institute, University Health Network, Toronto, Canada
    J Neurotrauma 26:209-25. 2009
    ..The Kogan's Old People (KOP) scale was used to assess attitudes toward elderly individuals among all members of the National Neurotrauma ..
  28. ncbi Anticonvulsive effects of kappa-opioid receptor modulation in an animal model of ethanol withdrawal
    A S Beadles-Bohling
    Department of Behavioral Neuroscience, Oregon Health and Science University Research Service, Portland, OR 97239, USA
    Genes Brain Behav 5:483-96. 2006
    ..Altered kappa-Opioid receptor (KOP-R) signaling has been observed in multiple seizure types; however, a role for this system in ethanol withdrawal ..
  29. doi Comparison of the discriminative stimulus effects of salvinorin A and its derivatives to U69,593 and U50,488 in rats
    Lisa E Baker
    Department of Psychology, Western Michigan University, Kalamazoo, MI, 49008, USA
    Psychopharmacology (Berl) 203:203-11. 2009
    ..Additionally, kappa opioid (KOP) receptor ligands may have therapeutic potential in the treatment of some neuropsychiatric conditions, including ..
  30. ncbi Effect of a conserved peptide derived from Kunitz domain of hepatitis B virus x protein on the cell cycle and apoptosis of HepG2 cells via the proteasome pathway
    Hong Liu
    Cancer Research Center, Qilu Hospital, Shandong University, Medical School of Shandong University, Jinan, Shandong 250012, China
    Chin Med J (Engl) 122:460-5. 2009
    ....
  31. ncbi Site-directed mutagenesis to identify key residues in structure-function relationship of winged bean chymotrypsin-trypsin inhibitor and 3-D structure prediction
    Sanhita Roy
    Department of Molecular and Human Genetics, Indian Institute of Chemical Biology, Kolkata, India
    Protein Pept Lett 18:471-9. 2011
    ..Finally, 3D model structure of WbCTI was designed using computer simulations which further emphasize the roles of L63 and R64 residues for dual inhibitory characteristics of WbCTI...
  32. ncbi p38 mitogen-activated protein kinase plays a key role in regulating MAPKAPK2 expression
    Tatsuhiko Sudo
    Antibiotics Laboratory and Bioarchitect Research Group, DRI, RIKEN, Wako, Saitama, Japan
    Biochem Biophys Res Commun 337:415-21. 2005
    ..from a p38alpha null mouse, namely, a parental cell line without p38alpha gene locus, knockout of p38alpha (KOP), Zeosin-resistant (ZKOP), revertant of p38alpha (RKOP), and Exip revertant (EKOP)...
  33. doi Chemical function-based pharmacophore development for novel, selective kappa opioid receptor agonists
    Nidhi Singh
    Department of Medicinal Chemistry, Laboratory for Applied Drug Design and Synthesis, The University of Mississippi, Mississippi 38677, USA
    J Mol Graph Model 27:131-9. 2008
    ..As a first step towards developing peripheral kappa opioid receptor (KOP) agonists, we have developed a quantitatively predictive chemical function-based pharmacophore model of selective ..
  34. pmc beta(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
    J Shrout
    College of Health Professions Cytology and Biosciences, Medical University of South Carolina, Charleston, SC 29425, USA
    Br J Cancer 98:1999-2005. 2008
    ..85; 95% confidence interval (CI)=0.69-0.94). We also observed that the expression ratio of B2M/Spint2 had the highest prognostic accuracy (AUC=0.87; 95% CI=0.71-0.96) of all potential two-gene combinations...
  35. pmc A Kunitz protease inhibitor from Dermacentor variabilis, a vector for spotted fever group rickettsiae, limits Rickettsia montanensis invasion
    Shane M Ceraul
    Department of Microbiology and Immunology, University of Maryland, Baltimore, School of Medicine, 685 West Baltimore Street, Howard Hall Suite 380, Baltimore, Maryland 21201, USA
    Infect Immun 79:321-9. 2011
    ..Collectively, our data demonstrate that DvKPI limits host cell invasion by Rickettsia montanensis, possibly through an association with the bacterium...
  36. ncbi Regulation of ProMMP-1 and ProMMP-3 activation by tissue factor pathway inhibitor-2/matrix-associated serine protease inhibitor
    C N Rao
    Department of Neurosurgery, University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Biochem Biophys Res Commun 255:94-8. 1999
    ..Collectively, our results suggest that TFPI-2/MSPI indirectly regulates MMP-1- and MMP-3-catalyzed matrix proteolysis by regulating the activation of proMMP-1 and proMMP-3...
  37. ncbi Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 1
    Kimitoshi Denda
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    J Biol Chem 277:14053-9. 2002
    ..These results suggest that serine protease binding sites of Kunitz I and Kunitz II are located close to each other and that proteolytic processing to generate HAI-1 with only one Kunitz domain regulates the activity of HAI-1...
  38. ncbi Lack of an interaction between orexinergic and opioid/nociceptinergic systems in rat cerebrocortical slices
    Kazuyoshi Hirota
    Department of Anesthesiology, University of Hirosaki School of Medicine, Hirosaki 036 8563, Japan
    Neurosci Lett 340:173-6. 2003
    ..D-Ala(2),N-Me-Phe(4),Gly(5)-ol]-enkephalin (DAMGO) (MOP-agonist) and spiradoline (KOP-agonist) significantly reduced OXA-evoked release with the concentration producing 50% of the maximal inhibition (..
  39. ncbi Expression of hepatocyte growth factor activator inhibitors (HAI-1 and HAI-2) in ovarian cancer
    Keiichiro Nakamura
    Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Int J Oncol 34:345-53. 2009
    ..These findings indicate that low HAI-1 and HAI-2 expression in ovarian cancer may be associated with poor prognosis. HAI-1 and HAI-2 could be considered a therapeutic target for treatment approaches in ovarian cancer...
  40. pmc Effect of age and gender on the progression of adult vascular dysfunction in a mouse model of fetal programming lacking endothelial nitric oxide synthase
    Giuseppe Chiossi
    Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TexasTX 77555, USA
    Am J Physiol Heart Circ Physiol 301:H297-305. 2011
    ..knockout (KO) and wild-type (WT) mice were cross bred to produce WT, KO, and heterozygous that developed in WT (KOP) or KO (KOM) mothers...
  41. doi Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants
    Irénée Kamdem
    Walloon Centre of Industrial Biology CWBI, Universite de Liege, Bd du Rectorat, 29, B40 P70, 4000, Liege, Belgium
    World J Microbiol Biotechnol 29:2259-70. 2013
    ..six morphological parts (MPs) of the "Williams Cavendish" banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography...
  42. pmc Effects of voluntary alcohol drinking on corticotropin-releasing factor and preprodynorphin mRNA levels in the central amygdala of Sardinian alcohol-preferring rats
    Yan Zhou
    Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA Electronic address
    Neurosci Lett 554:110-4. 2013
    ..differences in basal gene expression levels of CRF, CRF-R1, preprodynorphin (ppDyn) and kappa opioid receptor (KOP-r) between sP and sNP rats; specifically, mRNA levels of the above genes were measured in the central amygdala (CeA)..
  43. doi The role of the opioid system in alcohol dependence
    David J Nutt
    Imperial College London, London, UK
    J Psychopharmacol 28:8-22. 2014
    ..Further interrogation of how modulation of the opioid system, via use of MOP (mu), DOP (delta) and KOP (kappa) agents, restores the balance of a dysregulated system in alcohol dependence should increase our insight ..
  44. doi Epigenetic inactivation of SPINT2 is associated with tumor suppressive function in esophageal squamous cell carcinoma
    Dongli Yue
    The Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China The Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China
    Exp Cell Res 322:149-58. 2014
    b>Hepatocyte growth factor activator inhibitor type 2 (SPINT2), a Kunitz-type serine proteinase inhibitor, has been identified as a putative tumor suppressor gene silenced by promoter methylation...
  45. ncbi Hypertension, overweight and obesity in adolescents: the CRO-KOP study
    Ivan Pećin
    University of Zagreb, University Hospital Center Zagreb, Department of Internal Medicine, Zagreb, Croatia
    Coll Antropol 37:761-4. 2013
    ..54% (boys 2.2%; girls 4.9%). Our results confirmed positive relationship between overweight, obesity and hypertension starting from childhood pointing again the utmost importance of preventive measures beginning from early life...
  46. doi Characterization of a new Kunitz-type serine protease inhibitor from the hard tick Rhipicephalus hemaphysaloides
    Jie Cao
    Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China
    Arch Insect Biochem Physiol 84:104-13. 2013
    ..These results indicate that Rhipilin-2 is a novel Kunitz-type serine protease inhibitor involved in tick blood feeding. ..
  47. pmc Buprenorphine-elicited alteration of adenylate cyclase activity in human embryonic kidney 293 cells coexpressing κ-, μ-opioid and nociceptin receptors
    Pei Chen Wang
    Neuropsychiatric Center, National Health Research Institutes, Miaoli County, Taiwan
    J Cell Mol Med 19:2587-96. 2015
    Buprenorphine, a maintenance drug for heroin addicts, exerts its pharmacological function via κ- (KOP), μ-opioid (MOP) and nociceptin/opioid receptor-like 1 (NOP) receptors...
  48. doi Characterization of a Kunitz-type serine protease inhibitor from Solanum tuberosum having lectin activity
    Kunal R Shah
    Department of Bioinformatics and Structural Biology, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India
    Int J Biol Macromol 83:259-69. 2016
    ..This is the first report of a Kunitz-type protease inhibitor showing lectin like activity from a major crop plant and this makes PotHg an interesting candidate for further investigation...
  49. doi Evidence for nociceptin/orphanin FQ (NOP) but not µ (MOP), δ (DOP) or κ (KOP) opioid receptor mRNA in whole human blood
    M al-Hashimi
    Department of Cardiovascular Sciences, University of Leicester, Division of Anaesthesia, Critical Care and Pain Management, Leicester Royal Infirmary, Leicester LE2 7LX, UK
    Br J Anaesth 116:423-9. 2016
    ..using individual enriched immune cell populations we have failed to detect classical µ (MOP), δ (DOP) and κ (KOP) receptors...
  50. pmc Pectobacterium atrosepticum and Pectobacterium carotovorum Harbor Distinct, Independently Acquired Integrative and Conjugative Elements Encoding Coronafacic Acid that Enhance Virulence on Potato Stems
    Preetinanda Panda
    The Bio Protection Research CentreLincoln, New Zealand Plant Pathology, The New Zealand Institute for Plant and Food Research LimitedLincoln, New Zealand
    Front Microbiol 7:397. 2016
    ..In this study, the importance of horizontally acquired island 2 (HAI2), an ICE in the genome of Pectobacterium atrosepticum SCRI1043, was studied using a strain in which the entire ICE ..
  51. pmc Meta-analysis of DNA methylation biomarkers in hepatocellular carcinoma
    Cheng Zhang
    Department of Medical Oncology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang, China
    Oncotarget 7:81255-81267. 2016
    ..RASSF1A, GSTP1, p14, CDH1, APC, RUNX3, SOCS1, p15, MGMT, SFRP1, WIF1, PRDM2, DAPK1, RARβ, hMLH1, p73, DLC1, p53, SPINT2, OPCML and WT1) and HCC...
  52. ncbi Sequential NMR resonance assignment and structure determination of the Kunitz-type inhibitor domain of the Alzheimer's beta-amyloid precursor protein
    S L Heald
    Miles Research Center, Miles, Incorporated, West Haven, Connecticut 06516
    Biochemistry 30:10467-78. 1991
    ..In addition, the NMR spectra indicate that the rotation of the W21 ring in APPI is hindered, unlike Y21 in BPTI, showing a greater than 90% preference for one orientation in the hydrophobic groove...
  53. ncbi Cannabinoid CB1 receptor-mediated inhibition of noradrenaline release in guinea-pig vessels, but not in rat and mouse aorta
    T Schultheiss
    Institut fur Pharmakologie und Toxikologie, Rheinische Friedrich Wilhelms Universitat Bonn, Reuterstrasse 2b, 53113, Bonn, Germany
    Naunyn Schmiedebergs Arch Pharmacol 372:139-46. 2005
    ..from the guinea-pig aorta was comparable in size to that obtained with agonists at the histamine H3, kappa opioid (KOP) and ORL1 (NOP) receptor (1 or 10 microM, producing the respective near-maximum effects) whereas prostaglandin E2 1 ..
  54. ncbi Hepatocyte growth factor activator inhibitor-1 (HAI-1) is essential for the integrity of basement membranes in the developing placental labyrinth
    Bin Fan
    Department of Protein Engineering, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    Dev Biol 303:222-30. 2007
    ..Our study demonstrates the important role of HAI-1 in maintaining the integrity of basement membrane most likely by regulating extracellular proteolytic activities during placental development...
  55. ncbi [The efficacy of continuous hepatic arterial infusion therapy in association with multidisciplinary treatment of two late stage cancer patients]
    Takaho Watayo
    Dept of Surgery, Tokyo Metropolitan Ohtsuka Hospital
    Gan To Kagaku Ryoho 33:1788-91. 2006
    ..Dietary guidance in metabolic-nourishment therapy is very useful in the treatment of far advanced cancer patients...
  56. ncbi Tissue factor pathway inhibitor-2 as a frequently silenced tumor suppressor gene in hepatocellular carcinoma
    Chun Ming Wong
    Department of Pathology, S H Ho Foundation research Laboratories, Jockey Club Clinical Research Center, Pokfulam, Hong Kong, China
    Hepatology 45:1129-38. 2007
    ..Moreover, ectopic overexpression of TFPI-2 significantly suppressed the proliferation and invasiveness of HCC cells...
  57. doi Epigenetic inactivation and tumor suppressor activity of HAI-2/SPINT2 in gastric cancer
    Wenjie Dong
    Department of Gastroenterology, Rui Jin Hospital, Shanghai Jiao Tong University, Shanghai, China
    Int J Cancer 127:1526-34. 2010
    Hepatocyte growth factor (HGF) activator inhibitor type 2 (HAI-2/SPINT2) encodes Kunitz-type protease inhibitor that regulates HGF activity...
  58. doi Loss of the matriptase inhibitor HAI-2 during prostate cancer progression
    Christopher Bergum
    Department of Pharmacology, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Prostate 70:1422-8. 2010
    ..It has been proposed that the increase of matriptase with a concomitant loss of its inhibitors may play a critical role in cancer progression...
  59. doi Actions of the Kunitz-type serine protease inhibitor Amblyomin-X on VEGF-A-induced angiogenesis
    C C Drewes
    Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil
    Toxicon 60:333-40. 2012
    ..These data provide further mechanisms of actions of Kunitz-type SPI, corroborating their relevance as scientific tools in the design of therapeutic molecules...
  60. doi Structure of a post-translationally processed heterodimeric double-headed Kunitz-type serine protease inhibitor from potato
    Elisabeth M Meulenbroek
    Biophysical Structural Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands
    Acta Crystallogr D Biol Crystallogr 68:794-9. 2012
    ..PSPI has a β-trefoil fold and, based on the structure, two reactive-site loops bearing residues Phe75 and Lys95 were identified...
  61. doi Isolation and structural characterization of a (Kdo-isosteric) D-glycero-α-D-talo-oct-2-ulopyranosidonic acid (Ko) interlinking lipid A and core oligosaccharide in the lipopolysaccharide of Acinetobacter calcoaceticus NCTC 10305
    Ulrich Zahringer
    Research Center Borstel, Leibniz Center for Medicine and Biosciences, Parkallee 1 40, D 23845 Borstel, Germany
    Carbohydr Res 378:63-70. 2013
    ..Our results show that in A. calcoaceticusd-glycero-α-D-talo-oct-2-ulopyranosonic acid (DgαDt-Kop) represents the Kdo-related sugar interlinking the core-oligosaccharide and the lipid A backbone.
  62. doi Nature of SLC41A1 complexes: report on the split-ubiquitin yeast two hybrid assay
    Axel Nestler
    Institute of Veterinary Physiology, Freie Universitat Berlin, Berlin, Germany
    Magnes Res 26:56-66. 2013
    ..polytons (interactors appearing in the screen more than once) included: IER3IP1, PPIB, UPF0480 protein C15orf24, SPINT2, C14orf1/PEBP28, NIFIE14, YIPF6, and KCP2...
  63. doi Pharmacogenomic study of the role of the nociceptin/orphanin FQ receptor and opioid receptors in diabetic hyperalgesia
    Kris Rutten
    Grunenthal GmbH, Global Preclinical Drug Development, Department of Pain Pharmacology, Zieglerstrasse 6, 52078 Aachen, Germany Electronic address
    Eur J Pharmacol 741:264-71. 2014
    ..between different opioid receptors (µ-opioid peptide [MOP], δ-opioid peptide [DOP] and κ-opioid peptide [KOP]) and the nociceptin/orphanin FQ peptide (NOP) receptor in streptozotocin (STZ)-induced diabetic polyneuropathy, ..
  64. pmc Differential stabilities and sequence-dependent base pair opening dynamics of Watson-Crick base pairs with 5-hydroxymethylcytosine, 5-formylcytosine, or 5-carboxylcytosine
    Marta W Szulik
    Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Ingram Cancer Center, and Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37235, United States
    Biochemistry 54:1294-305. 2015
    ..At the oxidized base pair G(4):X(9), 5fC exhibited an increase in the imino proton exchange rate and the calculated kop. In all cases, minimal effects to imino proton exchange rates occurred at the neighboring base pair C(3):G(10)...
  65. ncbi Protease inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer's disease
    R E Tanzi
    Neurogenetics Laboratory, Massachusetts General Hospital, Boston 02115
    Nature 331:528-30. 1988
    ..The alternative APP mRNA is present in both brain and peripheral tissues of normal individuals and those with Alzheimer's disease, but its pattern of expression differs from that of the previously reported APP mRNA...
  66. ncbi Tissue factor pathway inhibitor-2 is a novel mitogen for vascular smooth muscle cells
    E Shinoda
    Third Division, Department of Internal Medicine, Kyoto University Hospital, Shogoin Kawaracho 54, Kyoto 6068507, Japan
    J Biol Chem 274:5379-84. 1999
    ..However, structurally similar TFPI-2 was found to have a mitogenic activity for the smooth muscle cell...
  67. ncbi A steric blockade model for inhibition of acetylcholinesterase by peripheral site ligands and substrate
    T L Rosenberry
    Department of Pharmacology, Mayo Clinic Jacksonville, FL 32224, USA
    Chem Biol Interact 119:85-97. 1999
    ..The 2nd-order phosphorylation rate constant kOP/K(OP) was decreased 3-fold by a high concentration of propidium and the 1st-order rate constant kOP increased ..
  68. ncbi Matrix localization of tissue factor pathway inhibitor-2/matrix-associated serine protease inhibitor (TFPI-2/MSPI) involves arginine-mediated ionic interactions with heparin and dermatan sulfate: heparin accelerates the activity of TFPI-2/MSPI toward plas
    Y Liu
    Department of Neurosurgery, The University of Texas M D Anderson Cancer Center, Houston, Texas, 77030, USA
    Arch Biochem Biophys 370:112-8. 1999
    ....
  69. ncbi Hepatocyte growth factor activator inhibitor type 1 is a specific cell surface binding protein of hepatocyte growth factor activator (HGFA) and regulates HGFA activity in the pericellular microenvironment
    H Kataoka
    Second Department of Pathology, Miyazaki Medical College, Kihara, Kiyotake, Miyazaki 889 1692, Japan
    J Biol Chem 275:40453-62. 2000
    ..HAI-1 complex. These findings shed light on a novel function of the integral membrane Kunitz-type inhibitor in the regulation of pericellular proteinase activity...
  70. pmc Matriptase and HAI-1 are expressed by normal and malignant epithelial cells in vitro and in vivo
    M Oberst
    Lombardi Cancer Center, Georgetown University Medical Center, 3970 Reservoir Road NW, Washington, DC 20007, USA
    Am J Pathol 158:1301-11. 2001
    ..The expression of the matriptase/HAI-1 system by malignant epithelial cells in vivo suggests a possible role for this protease in multiple aspects of the pathophysiology of epithelial malignancy, including invasion and metastasis...
  71. ncbi Expression of hepatocyte growth factor/scatter factor, its activator, inhibitors and the c-Met receptor in human cancer cells
    C Parr
    Metastasis Research Group, Department of Surgery, University of Wales College of Medicine, Cardiff, CF14 4XN, UK
    Int J Oncol 19:857-63. 2001
    ....
  72. ncbi Expression and localization of tissue factor pathway inhibitor-2 in normal and atherosclerotic human vessels
    James T B Crawley
    Thrombosis Research Institute, London, England
    Arterioscler Thromb Vasc Biol 22:218-24. 2002
    ....
  73. ncbi Kappa opioid receptor activation in the nucleus accumbens inhibits glutamate and GABA release through different mechanisms
    Gregory O Hjelmstad
    Department of Neurology, Ernest Gallo Clinic and Research Center, Emeryville, California 94143, USA
    J Neurophysiol 89:2389-95. 2003
    Through their actions in the nucleus accumbens (NAc), kappa opioid (KOP) receptors and their endogenous ligand, dynorphin, modify behaviors associated with the administration of drugs of abuse and are regulated by exposure to such drugs...
  74. ncbi Feeding induced by food deprivation is differentially reduced by opioid receptor antisense oligodeoxynucleotide probes in rats
    M M Hadjimarkou
    Department of Psychology and Neuropsychology Doctoral Sub Program, Queens College, City University of New York, 65 30 Kissena Blvd, Flushing, NY 11367, USA
    Brain Res 987:223-32. 2003
    ..oligodeoxynucleotide (AS ODN) probes directed against different exons of the mu (MOP), delta (DOP), kappa (KOP) and nociceptin (NOP) opioid peptide receptor genes have been able to differentially alter feeding responses ..
  75. ncbi We built it and they did not come: knowledge and attitudes of baccalaureate nursing students toward the elderly
    Marybeth Ryan
    School of Nursing, Adelphi University, Garden City, New York, USA
    J N Y State Nurses Assoc 35:5-9. 2004
    ..using two instruments: Palmore's revised Facts on Aging Quiz (FAQ 1) and Kogan's Attitudes Toward Old People Scale (KOP)...
  76. ncbi Kunitz-type serine protease inhibitor from potato (Solanum tuberosum L. cv. Jopung)
    Yoonkyung Park
    Research Center for Proteineous Materials, Department of Genetic Engineering, Chosun University, 375 Seosuk dong, Korea
    J Agric Food Chem 53:6491-6. 2005
    ..5 Da. This protease inhibitor suppressed over 50% the proteolytic activity at 400 microg/mL. These results suggest that AFP-J is an excellent candidate as a lead compound for the development of novel antiinfective agents...
  77. ncbi Nuclear translocation of H2RSP is impaired in regenerating intestinal epithelial cells of murine colitis model
    Seiji Naganuma
    Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, Japan
    Virchows Arch 448:354-60. 2006
    b>Hepatocyte growth factor activator inhibitor type 2-related small peptide (H2RSP) is a recently identified nuclear peptide that is abundantly expressed in the gastrointestinal tract...
  78. ncbi Defect of pro-hepatocyte growth factor activation by fibroblasts in idiopathic pulmonary fibrosis
    Sylvain Marchand-Adam
    INSERM Unit 700, Institut National de la Sante et de la Recherche Medicale, Faculte Xavier Bichat, Paris, France
    Am J Respir Crit Care Med 174:58-66. 2006
    ..Our hypothesis was that fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) had an altered capacity to activate pro-HGF in vitro compared with control fibroblasts...
  79. ncbi Dmt-Tic-NH-CH2-Bid (UFP-502), a potent DOP receptor agonist: in vitro and in vivo studies
    Raffaella Vergura
    Department of Experimental and Clinical Medicine, Section of Pharmacology, and Neuroscience Centre, University of Ferrara, via Fossato di Mortara 19, 44100 Ferrara, Italy
    Peptides 27:3322-30. 2006
    ..43) to recombinant DOP receptors displaying moderate selectivity over MOP and KOP. In CHO(hDOP) [(35)S]GTPgammaS binding and mouse vas deferens experiments, UFP-502 behaved as a potent (pEC(50) 10...
  80. ncbi Diversity in pathogenicity of Steinernema carpocapsae and its symbiotic bacterium for Spodoptera spp
    S Shahidi Noghabi
    Department of Crop Protection, Ghent University Coupure Links 653, BE 9000 Gent, Belgium
    Commun Agric Appl Biol Sci 71:689-700. 2006
    Pathogenicity of five isolates of Steinernema carpocapsae (GER: Germany, BUW: Becker Underwood, KOP: Koppert, CZ: Czech Republic, BEL: Belgium) were tested against the fourth instar larvae of the cotton leaf worm, Spodoptera littoralis ..
  81. ncbi Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase
    Kenji Kojima
    Laboratories of Enzyme Chemistry and Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto, Japan
    J Biol Chem 283:2478-87. 2008
    ....
  82. pmc Functional epigenomics approach to identify methylated candidate tumour suppressor genes in renal cell carcinoma
    M R Morris
    Cancer Research UK Renal Molecular Oncology Group, University of Birmingham, Birmingham B15 2TT, UK
    Br J Cancer 98:496-501. 2008
    ..This enabled us to identify HAI-2/SPINT2 as a novel epigenetically inactivated candidate RCC TSG...
  83. doi Crystal structure of textilinin-1, a Kunitz-type serine protease inhibitor from the venom of the Australian common brown snake (Pseudonaja textilis)
    Emma Karin I Millers
    School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Australia
    FEBS J 276:3163-75. 2009
    ..5 nm versus 0.053 nm) and could also account for an observed narrower binding specificity...
  84. pmc Dissection of platelet and myeloid cell defects by conditional targeting of the beta3-integrin subunit
    Elizabeth A Morgan
    Department of Medicine, Mallinkrodt Institute of Radiology, St Louis, Missouri, USA
    FASEB J 24:1117-27. 2010
    ..mice were generated to study the conditional deletion of beta3-integrin in platelets [knockout in platelets (KOP)] and myeloid cells [knockout in myeloid (KOM)]...
  85. pmc Lack of genotype effect on D1, D2 receptors and dopamine transporter binding in triple MOP-, DOP-, and KOP-opioid receptor knockout mice of three different genetic backgrounds
    Ji Hoon Yoo
    Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom
    Synapse 64:520-7. 2010
    ..Overall, these results indicate a lack of modulation of the dopaminergic system by the deletion of all three opioid receptors regardless of different background strains...
  86. pmc Neuroprotective potential of biphalin, multireceptor opioid peptide, against excitotoxic injury in hippocampal organotypic culture
    Maria Kawalec
    Molecular Biology Unit, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Str, 02 106 Warsaw, Poland
    Neurochem Res 36:2091-5. 2011
    Biphalin is a dimeric opioid peptide that exhibits affinity for three types of opioid receptors (MOP, DOP and KOP). Biphalin is undergoing intensive preclinical study...
  87. doi Persistent elevation of hepatocyte growth factor activator inhibitors in cholangiopathies affects liver fibrosis and differentiation
    Hsiang Po Huang
    Department of Medical Research, National Taiwan University Hospital, Taiwan
    Hepatology 55:161-72. 2012
    ..In addition, we showed that the hepatocyte growth factor activator, mitogen-activated protein kinase kinase 1, and phosphatidylinositol 3-kinase signaling pathways were involved in hepatic differentiation enhanced by HAI-2 knockdown...
  88. doi Glucagon counteracts interleukin-6-dependent gene expression by redundant action of Epac and PKA
    Christina Khouri
    Department of Biochemistry and Molecular Biology, RWTH Aachen University, Pauwelsstrase 30, D 52074 Aachen, Germany
    Biol Chem 392:1123-34. 2011
    ..The metabolic hormone glucagon interferes in IL-6-induced gene expression. This observation is indicative for a regulatory role of G-protein-coupled receptors in the IL-6-dependent inflammatory response...
  89. doi Inactivation of PbTopo IIIβ causes hyper-excision of the Pathogenicity Island HAI2 resulting in reduced virulence of Pectobacterium atrosepticum
    Bhanupratap R Vanga
    The New Zealand Institute for Plant and Food Research Limited, Private Bag 4704, Christchurch, New Zealand
    Mol Microbiol 84:648-63. 2012
    ..encoded on the chromosome of Pectobacterium atrosepticum strain SCRI1043 (Pba SCRI1043), is involved in excision of HAI2, a discrete ~100 kb region, from the Pba SCRI1043 chromosome...
  90. pmc Identification of unique sensitizing targets for anti-inflammatory CDDO-Me in metastatic melanoma by a large-scale synthetic lethal RNAi screening
    Yong Qin
    Department of Melanoma Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Pigment Cell Melanoma Res 26:97-112. 2013
    ..Based on screening results, five unique genes (GNPAT, SUMO1, SPINT2, FLI1, and SSX1) significantly potentiated the growth inhibitory effects of CDDO-Me and induced apoptosis in A375, ..
  91. pmc Inducible Sterilization of Zebrafish by Disruption of Primordial Germ Cell Migration
    Ten Tsao Wong
    Department of Animal Sciences, Purdue University, West Lafayette, Indiana, United States of America
    PLoS ONE 8:e68455. 2013
    ..To better visualize the PGCs, the Tg(hsp70:sdf1a-nanos3, EGFP) fish were crossed with another transgenic line, Tg(kop:DsRed-nanos3), that expresses DsRed driven by the PGC-specific kop promoter...
  92. pmc A genetic polymorphism of the endogenous opioid dynorphin modulates monetary reward anticipation in the corticostriatal loop
    Mikhail Votinov
    Social, Cognitive and Affective Neuroscience Unit, Department of Basic Psychological Research and Research Methods, Faculty of Psychology, University of Vienna, Vienna, Austria
    PLoS ONE 9:e89954. 2014
    The dynorphin/κ-opioid receptor (KOP-R) system has been shown to play a role in different types of behavior regulation, including reward-related behavior and drug craving...
  93. doi Endomorphin analogues with mixed μ-opioid (MOP) receptor agonism/δ-opioid (DOP) receptor antagonism and lacking β-arrestin2 recruitment activity
    Jun Cai
    School of Pharmacy, Nanjing Medical University, Nanjing 211166, China
    Bioorg Med Chem 22:2208-19. 2014
    ..5-316), and potent functional MOP agonism (GPI, IC50=0.274-249nM) without DOP and κ-opioid (KOP) receptor agonism...
  94. doi Knockout subtraction autoradiography: a novel ex vivo method to detect heteromers finds sparse KOP receptor/DOP receptor heterodimerization in the brain
    Ji Hoon Yoo
    Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Eur J Pharmacol 731:1-7. 2014
    ..model, which we have named Knockout Subtraction Autoradiography, to determine if heterodimerisation of the kappa (KOP) and delta opioid (DOP) receptors occurs, as evidence from binding studies in cell systems suggest they are present ..
  95. pmc K303⁶·⁵⁸ in the μ opioid (MOP) receptor is important in conferring selectivity for covalent binding of β-funaltrexamine (β-FNA)
    Kelly M DiMattio
    Center for Substance Abuse Research and Department of Pharmacology, Temple University School of Medicine, 3500 N Broad Street, MERB 851, Philadelphia, PA 19140, United States Electronic address
    Eur J Pharmacol 748:93-100. 2015
    ..FNA) is an irreversible μ opioid (MOP) receptor antagonist and a reversible agonist of κ opioid (KOP) receptor. β-FNA binds covalently to the MOP receptor at Lys233(5.39), which is conserved among opioid receptors...
  96. pmc Discovery of the first small-molecule opioid pan antagonist with nanomolar affinity at mu, delta, kappa, and nociceptin opioid receptors
    Nurulain T Zaveri
    Astraea Therapeutics, 320 Logue Avenue, Suite 142, Mountain View, California 94043, United States
    ACS Chem Neurosci 6:646-57. 2015
    ..scaffold is a known pharmacophore for mu opioid (MOP), kappa opioid (KOP), and delta opioid (DOP) receptor antagonists; however, it has not been explored in nociceptin opioid (NOP/ORL-1) ..
  97. doi Ring size in cyclic endomorphin-2 analogs modulates receptor binding affinity and selectivity
    Justyna Piekielna
    Department of Biomolecular Chemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland
    Org Biomol Chem 13:6039-46. 2015
    ..The influence of the ring size on binding to the MOP, DOP and KOP opioid receptors was studied...
  98. ncbi Aberrant methylation of promoter region of SPINT2/HAI-2 gene: an epigenetic mechanism in hepatitis C virus-induced hepatocarcinogenesis
    Ragaa A Ramadan
    1 Department of Chemical Pathology, Alexandria University, Alexandria, Egypt
    Genet Test Mol Biomarkers 19:399-404. 2015
    ..Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms involved in the pathogenesis of hepatocellular carcinoma (HCC)...
  99. doi Construct development, description and initial validation of the Knowledge about Older Patients Quiz (KOP-Q) for nurses
    Jeroen Dikken
    Research Group for the Chronically Ill, Faculty of Health Care, University of Applied Sciences, Utrecht, Utrecht, The Netherlands Electronic address
    Nurse Educ Today 35:e54-9. 2015
    ..A first step is understanding nurses' current knowledge. This can be achieved by using a measurement instrument with good validity and reliability...
  100. doi Genome-wide DNA methylation analysis in hepatocellular carcinoma
    Nobuhisa Yamada
    Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602 8566, Japan
    Oncol Rep 35:2228-36. 2016
    ..to three known tumor-suppressor genes (APC, CDKN2A, and GSTP1), eight genes (AKR1B1, GRASP, MAP9, NXPE3, RSPH9, SPINT2, STEAP4, and ZNF154) were significantly hypermethylated and downregulated in the HCC tumors compared to the non-..
  101. ncbi Discriminative Stimulus Properties of Opioid Ligands: Progress and Future Directions
    Eduardo R Butelman
    Laboratory on the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, Box 171, New York, NY, 10065, USA
    Curr Top Behav Neurosci . 2016
    Opioid receptors (MOP-r, KOP-r, DOP-r, as well as NOP-r) and their endogenous neuropeptide agonist systems are involved in diverse neurobiological and behavioral functions, in health and disease...

Research Grants19

  1. THOMAS EDWARD PRISINZANO; Fiscal Year: 2016
    ..A growing body of evidence has shown that ? opioid (KOP) receptors are involved in the modulation of some of the abuse related effects of psychostimulants...
  2. Yong Zhang; Fiscal Year: 2014
    ..To determine whether and how adolescent oxycodone self-administration alters mRNA levels of Pdyn, Penk1, MOP-r and KOP-r, MOP-r and KOP-r receptor density in specific brain regions of the mesocorticolimbic and nigrostriatal ..
  3. Developing a ClO2 Compliance Service: Minimizing Water Borne Microbial Infection
    SPENDEL U WOLFGANG; Fiscal Year: 2011
    ....
  4. NOVEL SERINE PROTEASE IN INVASIVE CARCINOMAS
    Robert Dickson; Fiscal Year: 2000
    ..In addition, they will focus the research of a protease biochemist early in his career on the problem of metastasis. ..
  5. Targeting Opioid Receptor Heterodimers for Pain Treatment
    Selena Bartlett; Fiscal Year: 2007
    ..first to show that the compound 6'-GNTI interacts specifically at the DOP-R and kappa opioid peptide receptor (KOP-R) heterodimeric complex (Waldhoer et al., 2005)...
  6. 'Latinos' Health Literacy, Social Support, and OH-KOP"
    Maria Rosa Watson; Fiscal Year: 2006
    ..that will be assessed and further detailed include: A previously developed oral health knowledge and practices (KOP) survey instrument, developed in the Inner City Hispanic Community of Mount Pleasant in Washington D.C...
  7. MOLECULAR REGULATION OF ARTERIAL THROMBOSIS
    Robert Simari; Fiscal Year: 2009
    ..Specific Aim 2: To define the role of endothelial-derived TFPI on vascular thrombosis. Specific Aim 3: To define the role of endothelial-derived TFPI on vascular remodeling including angiogenesis. ..
  8. Controlling Apoptosis in Oligondendrocytes
    Pamela Knapp; Fiscal Year: 2005
    ..Specific Aim 4: Biochemical and histological techniques are used to determine whether pathways which are independent of caspase-3 play a significant role in cell loss after SCI. ..
  9. NOVEL PHARMACOTHERAPY FOR TREATMENT OF COCAINE ADDICTION
    Frank Carroll; Fiscal Year: 2008
    ..abstract_text> ..
  10. Design and Development of Pharmacotherapies for Treating Stimulant Abuse
    Frank Ivy Carroll; Fiscal Year: 2010
    ..3) To continue on a limited basis our development of selected 3-phenyltropane analogs as second-generation pharmacotherapies to treat cocaine addiction. ..
  11. Role of insulin-like signaling in the hookworm infective process
    John M Hawdon; Fiscal Year: 2010
    ..This project will also provide insight into the basic molecular mechanisms operating during infection and the establishment of parasitic relationships that are relevant to other parasitic nematode infections of human significance. ..
  12. Salvinorin A: A Structurally Novel Opioid Agonist Lead
    Christopher McCurdy; Fiscal Year: 2004
    ..Results of this work will direct further analog development of Salvinorin A (a next generation) and further define and refine our proposed computer binding and field models. ..
  13. GHB: Discrimination and effects on learning
    Lisa Baker; Fiscal Year: 2003
    ..Behavior under these assays is sensitive to other abused drugs. Therefore, these assays may be of value in characterizing the effects of GHB. ..
  14. NOVEL PHARMACOTHERAPIES FOR COCAINE DEPENDENCE
    Frank Carroll; Fiscal Year: 2001
    ..An ideal candidate compound would not only have efficacy in the preclinical models but would be safe, orally active, and potent, would not show tolerance or sensitization, and if abusable, would be less so than cocaine. ..
  15. Opioids Modulate Oligodendrocyte Development & Function
    Pamela Knapp; Fiscal Year: 2006
    ..Techniques used include cell culture, immunostaining, in situ hybridization, immunoblot, confocal microscopy and adenoviral transfection. ..
  16. Proteomic Analysis of Hookworm Larval Activation
    John Hawdon; Fiscal Year: 2006
    ..These proteins represent potential vaccine antigens and drug targets for the prevention of hookworm infection and the control of hookworm disease. [unreadable] [unreadable]..
  17. Plasticity of Kappa Opioid Function in Reward Circuitry
    GREGORY HJELMSTAD; Fiscal Year: 2007
    ..Kappa opioid (KOP) receptors have a wide distribution throughout the CNS that largely parallels that of MOP receptors including ..
  18. Deregulation of Matriptase in Breast Cancer Cells
    Chen Yong Lin; Fiscal Year: 2008
    ..These studies could lead to new perspectives on the deregulation of this physiological protease in breast cancer and provide new avenues for diagnosis and intervention of the disease. ..
  19. COCAINE: A STUDY OF THE BIOCHEMICAL MECHANISM OF ACTION
    Frank Carroll; Fiscal Year: 2007
    ..abstract_text> ..

Patents1

  1. Human cDNAs and proteins and uses thereof
    Patent Number: WO03046180-A2; Date:2003-06-05