SPF45

Summary

Gene Symbol: SPF45
Description: RNA binding motif protein 17
Alias: SPF45, splicing factor 45, 45 kDa-splicing factor, splicing factor 45kDa
Species: human

Top Publications

  1. pmc Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 452:713-8. 2008
  2. ncbi Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45
    María José Lallena
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Cell 109:285-96. 2002
  3. doi Extensive cryptic splicing upon loss of RBM17 and TDP43 in neurodegeneration models
    Qiumin Tan
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Hum Mol Genet . 2016
  4. ncbi Rational Design of Cyclic Peptide Inhibitors of U2AF Homology Motif (UHM) Domains To Modulate Pre-mRNA Splicing
    Pravin Kumar Ankush Jagtap
    Institute of Structural Biology, Helmholtz Zentrum Munchen, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany
    J Med Chem 59:10190-10197. 2016
  5. pmc Long noncoding RNA Saf and splicing factor 45 increase soluble Fas and resistance to apoptosis
    Olga Villamizar
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois, USA
    Oncotarget 7:13810-26. 2016
  6. pmc Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins
    Angela Crisci
    Department of Cell Biology, Faculty of Sciences, University of Geneva, CH 1211 Geneva 4, Switzerland
    Nucleic Acids Res 43:10456-73. 2015
  7. doi Structural basis of the phosphorylation dependent complex formation of neurodegenerative disease protein Ataxin-1 and RBM17
    Eunji Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
    Biochem Biophys Res Commun 449:399-404. 2014
  8. pmc Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex
    Sarah Loerch
    From the Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642 and
    J Biol Chem 289:17325-37. 2014
  9. pmc Genome-wide association analysis identifies 11 risk variants associated with the asthma with hay fever phenotype
    Manuel A R Ferreira
    QIMR Berghofer Medical Research Institute, Brisbane, Australia Electronic address
    J Allergy Clin Immunol 133:1564-71. 2014
  10. ncbi Large-scale genetic fine mapping and genotype-phenotype associations implicate polymorphism in the IL2RA region in type 1 diabetes
    Christopher E Lowe
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, UK
    Nat Genet 39:1074-82. 2007

Research Grants

  1. Regulation of Ovarian Cancer Multidrug Resistance by MAP Kinases
    Scott T Eblen; Fiscal Year: 2013
  2. Huda Y Zoghbi; Fiscal Year: 2014
  3. Harry T Orr; Fiscal Year: 2016
  4. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009

Scientific Experts

  • BRENTON GRAVELEY
  • J D Cooper
  • William L Perry
  • Huda Y Zoghbi
  • Harry T Orr
  • Scott T Eblen
  • Yuying Liu
  • Adnan M Al-Ayoubi
  • Pravin Kumar Ankush Jagtap
  • Olga Villamizar
  • Qiumin Tan
  • Angela Crisci
  • Manuel A R Ferreira
  • Eunji Kim
  • Sarah Loerch
  • Hui Zheng
  • Marie Helene Teiten
  • Michael Sattler
  • Shaojuan Lai
  • Naoki Horikoshi
  • Ying Jin
  • Janghoo Lim
  • Lorenzo Corsini
  • Christopher E Lowe
  • Ahmad Sami Chaouki
  • Juan Valcarcel
  • Layal S Sayegh
  • Reinhard Luhrmann
  • Cindy L Will
  • Vitaliy V Bondar
  • Oliver Demmer
  • Antonia De Maio
  • Derek A Persons
  • Jeehye Park
  • Hsiang Chih Lu
  • Christopher B Chambers
  • Yan Gao
  • Tobias G Kapp
  • Divita Garg
  • Zhandong Liu
  • Ying Wooi Wan
  • Roy V Sillitoe
  • Andrew Wilber
  • Hari Krishna Yalamanchili
  • Xiuyun Liu
  • Horst Kessler
  • Joshua J White
  • Janice M Riberdy
  • Janardhan Sampath
  • Monika Raabe
  • Henning Urlaub
  • Jean Christophe Rain
  • Angela Kramer
  • Ivona Bagdiul
  • Flore Raleff
  • Michael Hunter
  • Shyamali C Dharmage
  • Joyce Y Tung
  • Colin F Robertson
  • Clara L Kielkopf
  • David L Duffy
  • Yoonji Lee
  • Wei Ang
  • Sun Choi
  • John L Hopper
  • Svetlana Baltic
  • Michael J Abramson
  • A John Henderson
  • Patrick G Holt
  • Peter D Sly
  • Alan James
  • Nicholas G Martin
  • Jennie Hui
  • Grant W Montgomery
  • Ji Joon Song
  • Andrew C Heath
  • Philip J Thompson
  • David M Evans
  • Pamela A Madden
  • Alexandre Maucuer
  • Craig Pennell
  • Valérie Manceau
  • Nicholas Eriksson
  • David A Hinds
  • Minh Bui
  • Michael R Green
  • Peter Le Souef
  • Loren Price
  • Raquel Granell
  • Bill Musk

Detail Information

Publications22

  1. pmc Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 452:713-8. 2008
    ..This model provides mechanistic insight into the molecular pathogenesis of SCA1 as well as other polyglutamine diseases...
  2. ncbi Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45
    María José Lallena
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Cell 109:285-96. 2002
    ..Here we show that U2AF interacts with the Py-tract and downstream AG, whereas the spliceosomal protein SPF45 interacts with the upstream AG and activates it for the second catalytic step of the splicing reaction...
  3. doi Extensive cryptic splicing upon loss of RBM17 and TDP43 in neurodegeneration models
    Qiumin Tan
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Hum Mol Genet . 2016
    ..We propose that repression of cryptic splicing by RBPs is critical for neuronal health and survival. CrypSplice is available at www.liuzlab.org/CrypSplice...
  4. ncbi Rational Design of Cyclic Peptide Inhibitors of U2AF Homology Motif (UHM) Domains To Modulate Pre-mRNA Splicing
    Pravin Kumar Ankush Jagtap
    Institute of Structural Biology, Helmholtz Zentrum Munchen, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany
    J Med Chem 59:10190-10197. 2016
    ..b>Splicing factor 45 kDa (SPF45) is an alternative splicing factor implicated in breast and lung cancers, and splicing regulation ..
  5. pmc Long noncoding RNA Saf and splicing factor 45 increase soluble Fas and resistance to apoptosis
    Olga Villamizar
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois, USA
    Oncotarget 7:13810-26. 2016
    ..We show that Saf is localized in the nucleus where it interacts with Fas receptor pre-mRNA and human splicing factor 45 (SPF45) to facilitate alternative splicing and exclusion of exon 6...
  6. pmc Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins
    Angela Crisci
    Department of Cell Biology, Faculty of Sciences, University of Geneva, CH 1211 Geneva 4, Switzerland
    Nucleic Acids Res 43:10456-73. 2015
    ..In addition, these findings may have implications for alternative splicing decisions. ..
  7. doi Structural basis of the phosphorylation dependent complex formation of neurodegenerative disease protein Ataxin-1 and RBM17
    Eunji Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
    Biochem Biophys Res Commun 449:399-404. 2014
    ..Furthermore, the structure of RBM17 and pATXN1 peptide might be utilized to target RBM17-ATXN1 interaction to modulate SCA1 pathogenesis. ..
  8. pmc Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex
    Sarah Loerch
    From the Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642 and
    J Biol Chem 289:17325-37. 2014
    ..In conjunction with the previously identified, UHM/ULM-mediated complexes of U2AF(65) and SPF45 with SF3b155, this work demonstrates the capacity of SF3b155 to offer a platform for coordinated recruitment of ..
  9. pmc Genome-wide association analysis identifies 11 risk variants associated with the asthma with hay fever phenotype
    Manuel A R Ferreira
    QIMR Berghofer Medical Research Institute, Brisbane, Australia Electronic address
    J Allergy Clin Immunol 133:1564-71. 2014
    ..Previous analyses of family data from the Tasmanian Longitudinal Health Study provide evidence that this phenotype has a stronger genetic cause than asthma without hay fever...
  10. ncbi Large-scale genetic fine mapping and genotype-phenotype associations implicate polymorphism in the IL2RA region in type 1 diabetes
    Christopher E Lowe
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, UK
    Nat Genet 39:1074-82. 2007
    ..635). Furthermore, we have associated IL2RA T1D susceptibility genotypes with lower circulating levels of the biomarker, soluble IL-2RA (P = 6.28 x 10(-28)), suggesting that an inherited lower immune responsiveness predisposes to T1D...
  11. pmc Phosphorylation of the alternative mRNA splicing factor 45 (SPF45) by Clk1 regulates its splice site utilization, cell migration and invasion
    Yuying Liu
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 173 Ashley Ave, Charleston, SC 29425, USA
    Nucleic Acids Res 41:4949-62. 2013
    ..The alternative splicing factor 45 (SPF45) is overexpressed in cancer, although few biological effects of SPF45 are known, and few splicing ..
  12. doi Identification of differentially expressed proteins in curcumin-treated prostate cancer cell lines
    Marie Helene Teiten
    Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hopital Kirchberg, Luxembourg
    OMICS 16:289-300. 2012
    ..Taken together, these data support the hypothesis that curcumin is an interesting chemopreventive agent as it modulates the expression of proteins that potentially contribute to prostate carcinogenesis...
  13. pmc Mitogen-activated protein kinase phosphorylation of splicing factor 45 (SPF45) regulates SPF45 alternative splicing site utilization, proliferation, and cell adhesion
    Adnan M Al-Ayoubi
    Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, South Carolina, USA
    Mol Cell Biol 32:2880-93. 2012
    ..signal-regulated kinase 2 (ERK2) that can utilize ATP analogs, we have identified the alternative mRNA splicing factor 45 (SPF45), which is overexpressed in cancer, as a novel coimmunoprecipitating ERK2 substrate...
  14. pmc 14-3-3 Binding to ataxin-1(ATXN1) regulates its dephosphorylation at Ser-776 and transport to the nucleus
    Shaojuan Lai
    Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 286:34606-16. 2011
    ..In the nucleus, we propose that dephosphorylation of pS776-ATXN1 by PP2A regulates the interaction of ATXN1 with the splicing factors RBM17 and U2AF65...
  15. pmc Variant of TYR and autoimmunity susceptibility loci in generalized vitiligo
    Ying Jin
    Human Medical Genetics Program, School of Medicine, University of Colorado, P O Box 6511, Mailstop 8300, Aurora, CO 80045, USA
    N Engl J Med 362:1686-97. 2010
    ..Generalized vitiligo is an autoimmune disease characterized by melanocyte loss, which results in patchy depigmentation of skin and hair, and is associated with an elevated risk of other autoimmune diseases...
  16. doi Holliday junction-binding activity of human SPF45
    Naoki Horikoshi
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Genes Cells 15:373-83. 2010
    b>SPF45 is considered to be a bifunctional protein that functions in splicing and DNA repair...
  17. ncbi U2AF-homology motif interactions are required for alternative splicing regulation by SPF45
    Lorenzo Corsini
    European Molecular Biology Laboratory EMBL, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 14:620-9. 2007
    ..Here we report that the splicing factor SPF45 regulates alternative splicing of the apoptosis regulatory gene FAS (also called CD95)...
  18. pmc Drosophila SPF45: a bifunctional protein with roles in both splicing and DNA repair
    Ahmad Sami Chaouki
    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS Genet 2:e178. 2006
    The sequence of the SPF45 protein is significantly conserved, yet functional studies have identified it as a splicing factor in animal cells and as a DNA-repair protein in plants...
  19. ncbi Human splicing factor SPF45 (RBM17) confers broad multidrug resistance to anticancer drugs when overexpressed--a phenotype partially reversed by selective estrogen receptor modulators
    William L Perry
    Lilly Research Laboratories, Eli Lilly and Co, Indianapolis, Indiana 46285, USA
    Cancer Res 65:6593-600. 2005
    The splicing factor SPF45 (RBM17) is frequently overexpressed in many solid tumors, and stable expression in HeLa cells confers resistance to doxorubicin and vincristine...
  20. pmc Human SPF45, a splicing factor, has limited expression in normal tissues, is overexpressed in many tumors, and can confer a multidrug-resistant phenotype to cells
    Janardhan Sampath
    Cancer Research Division, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Am J Pathol 163:1781-90. 2003
    ..novel drug-resistant genes in cyclophosphamide-resistant EMT6 mouse mammary tumors led us to the identification of SPF45. Simultaneously, other groups identified SPF45 as a component of the spliceosome that is involved in alternative ..
  21. ncbi Sex, AGility, and the regulation of alternative splicing
    Brenton R Graveley
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington 06030, USA
    Cell 109:409-12. 2002
    ..detailed examination of the Drosophila Sex-lethal gene has led to two significant discoveries-the role of the splicing factor SPF45 in defining the site of exon ligation, and that alternative splicing can be regulated at the second step.
  22. pmc Meta-analysis of genome-wide association study data identifies additional type 1 diabetes risk loci
    Jason D Cooper
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Nat Genet 40:1399-401. 2008
    ..7 x 10(-12)), 10p15 (PRKCQ, P = 3.7 x 10(-9)), 15q24 (CTSH, P = 3.2 x 10(-15)) and 22q13 (C1QTNF6, P = 2.0 x 10(-8))...

Research Grants4

  1. Regulation of Ovarian Cancer Multidrug Resistance by MAP Kinases
    Scott T Eblen; Fiscal Year: 2013
    ..Over-expression of the splicing factor SPF45 in ovarian cancer cells results in a tamoxifen sensitive drug-resistant phenotype...
  2. Huda Y Zoghbi; Fiscal Year: 2014
    ..The knowledge we gain from these studies are likely to shed light on other neurodegenerative diseases, RNA biology, and role of RNA processing in neurobiology. ..
  3. Harry T Orr; Fiscal Year: 2016
    ..The long-term goal of this work is to use information obtained about these pathways to identify novel avenues for therapeutic development. ) ..
  4. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009
    ....