SPAG4

Summary

Gene Symbol: SPAG4
Description: sperm associated antigen 4
Alias: CT127, SUN4, sperm-associated antigen 4 protein, SUN domain-containing protein 4, Sad1 and UNC84 domain containing 4, acrosomal protein ACR55, cancer/testis antigen 127, outer dense fiber-associated protein SPAG4, sperm tail protein
Species: human

Top Publications

  1. ncbi Identification of differentially expressed genes in the testis of Sprague-Dawley rats treated with di(n-butyl) phthalate
    Ju Young Ryu
    Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University, San 30, Jangjun dong, Gumjung ku, Busan, South Korea
    Toxicology 234:103-12. 2007
  2. ncbi Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme
    X Shao
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, T2N 4N1, Canada
    Dev Biol 211:109-23. 1999
  3. ncbi Changes in renal gene expression associated with induced ochratoxicosis in chickens: activation and deactivation of transcripts after varying durations of exposure
    Cynthia P Zeferino
    Division of Animal Sciences, University of Missouri UM, Columbia 65211, United States
    Poult Sci . 2016
  4. pmc Comparison of Normal and Pre-Eclamptic Placental Gene Expression: A Systematic Review with Meta-Analysis
    O Brew
    University of West London, Brentford, Middlesex, United Kingdom
    PLoS ONE 11:e0161504. 2016
  5. pmc SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells
    Chung Hsin Yeh
    Division of Urology, Department of Surgery, Shin Kong Wu Ho Su Memorial Hospital, Taipei, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan
    PLoS ONE 10:e0120722. 2015
  6. doi Hypoxia regulates the sperm associated antigen 4 (SPAG4) via HIF, which is expressed in renal clear cell carcinoma and promotes migration and invasion in vitro
    Karl Xaver Knaup
    Department of Nephrology and Hypertension, University of Erlangen Nuremberg, Erlangen, Germany Nikolaus Fiebiger Center, University of Erlangen Nuremberg, Erlangen, Germany
    Mol Carcinog 53:970-8. 2014
  7. doi Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates cytokinesis, and its expression correlates with the prognosis of renal cell carcinoma
    Kumi Shoji
    Division of Nephrology and Endocrinology, University of Tokyo, Tokyo, Japan
    Am J Pathol 182:2191-203. 2013
  8. ncbi Identification of a novel gene SRG4 expressed at specific stages of mouse spermatogenesis
    Xiao Wei Xing
    Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai, China
    Acta Biochim Biophys Sin (Shanghai) 36:351-9. 2004
  9. ncbi Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples
    Roberto Yunes
    Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA, USA
    Biocell 27:29-36. 2003
  10. ncbi Expression of Tsga10 sperm tail protein in embryogenesis and neural development: from cilium to cell division
    Babak Behnam
    The Galton Laboratory, Department of Biology, University College London, London NW1 2HE, UK
    Biochem Biophys Res Commun 344:1102-10. 2006

Research Grants

  1. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002

Scientific Experts

  • Abraham L Kierszenbaum
  • Frans A van der Hoorn
  • Babak Behnam
  • Xiao Wei Xing
  • Ju Young Ryu
  • Young Jun Lee
  • Hyung Sik Kim
  • O Brew
  • Cynthia P Zeferino
  • Chung Hsin Yeh
  • Karl Xaver Knaup
  • X Shao
  • Kumi Shoji
  • Xian zhen Jiang
  • Lu Yun Li
  • Gang Liu
  • Guang Xiu Lu
  • Martin P Kracklauer
  • Seung Jun Kwack
  • Yoshitake Nishimune
  • Hiromitsu Tanaka
  • Naoko Iguchi
  • Carlos Egydio de Carvalho
  • Byung Mu Lee
  • Sam Kacew
  • Ying Zheng
  • Nicholas A Salmon
  • Carolyn J Fitzgerald
  • Claire Kennedy
  • Jun Jiang Fu
  • Guido KappĂ©
  • George E Rottinghaus
  • A Woodman
  • Kevin D Wells
  • Ana Silvia A M T Moura
  • Roberto Yunes
  • M H F Sullivan
  • Kouichi Kitamura
  • Heather A Zarsky
  • David R Ledoux
  • Ying Hung Lin
  • Ya Yun Wang
  • Yasushi Miyagawa
  • Ding Yen Lin
  • Han Sun Chiang
  • Mei Feng Chen
  • Ying Yu Wu
  • Tsung Hsuan Lai
  • Pao Lin Kuo
  • Thomas Hackenbeck
  • Michael Sean Wiesener
  • Ruth Elisabeth Schietke
  • Kai Uwe Eckardt
  • Juergen Behrens
  • Tilmann Jobst-Schwan
  • Ingrid Wacker
  • Kerstin Amann
  • Christina Warnecke
  • Hiroshi Nojima
  • Yoshihiro Nakamura
  • Juliana Monti
  • Bernd Klanke
  • Takehiko Wada
  • Yukio Homma
  • Takeshi Hasegawa
  • Hiroyuki Aburatani
  • Haruki Kume
  • Tetsuhiro Tanaka
  • Reiko Inagi
  • Masashi Fukayama
  • Ichiro Manabe
  • Takamoto Ohse
  • Takashi Murayama
  • Tatsuhiko Kodama
  • Akiteru Goto
  • Takashi Sakurai
  • Masaomi Nangaku
  • Imari Mimura
  • Kul Lea Park
  • Ming gang Yang
  • Li Hua Huang
  • Chang Qi Li
  • Sik Yoon
  • Mee Young Ahn
  • Dosik Min
  • Dwight Romanowicz
  • Kathleen M Beckingham
  • Xin Chen
  • Janice A Fischer
  • Margaret T Fuller

Detail Information

Publications30

  1. ncbi Identification of differentially expressed genes in the testis of Sprague-Dawley rats treated with di(n-butyl) phthalate
    Ju Young Ryu
    Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University, San 30, Jangjun dong, Gumjung ku, Busan, South Korea
    Toxicology 234:103-12. 2007
    ..and 3 genes (LDHA, lactate dehydrogenase A; Spag4, sperm associated antigen 4 and PBR, peripheral-type benzodiazepine receptor) were found to be involved in ..
  2. ncbi Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme
    X Shao
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, T2N 4N1, Canada
    Dev Biol 211:109-23. 1999
    ..Here we report the cloning and characterization of a new Odf1-interacting protein, Spag4. Spag4 mRNA is spermatid specific, and the 49-kDa Spag4 protein complexes specifically with Odf1, but not Odf2, ..
  3. ncbi Changes in renal gene expression associated with induced ochratoxicosis in chickens: activation and deactivation of transcripts after varying durations of exposure
    Cynthia P Zeferino
    Division of Animal Sciences, University of Missouri UM, Columbia 65211, United States
    Poult Sci . 2016
    ..In contrast, the genes deactivated at 7 d were SPAG4 and LOC100857131, at 14 d were LOC771469, NKX2-1, NKX2-8, FOXO1, MyHC and CLDN18 and at 21 d was XPC...
  4. pmc Comparison of Normal and Pre-Eclamptic Placental Gene Expression: A Systematic Review with Meta-Analysis
    O Brew
    University of West London, Brentford, Middlesex, United Kingdom
    PLoS ONE 11:e0161504. 2016
    ..05), 10 of which (LEP, HTRA4, SPAG4, LHB, TREM1, FSTL3, CGB, INHA, PROCR, and LTF) were significant at p < 0.001...
  5. pmc SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells
    Chung Hsin Yeh
    Division of Urology, Department of Surgery, Shin Kong Wu Ho Su Memorial Hospital, Taipei, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan
    PLoS ONE 10:e0120722. 2015
    ..Sperm-associated antigen 4 (SPAG4; also known as SUN4) belongs to the SUN family of proteins and acts as a linker protein between nucleoskeleton and ..
  6. doi Hypoxia regulates the sperm associated antigen 4 (SPAG4) via HIF, which is expressed in renal clear cell carcinoma and promotes migration and invasion in vitro
    Karl Xaver Knaup
    Department of Nephrology and Hypertension, University of Erlangen Nuremberg, Erlangen, Germany Nikolaus Fiebiger Center, University of Erlangen Nuremberg, Erlangen, Germany
    Mol Carcinog 53:970-8. 2014
    ..Here, we are the first to report the hypoxic regulation of the sperm associated antigen 4 (SPAG4)...
  7. doi Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates cytokinesis, and its expression correlates with the prognosis of renal cell carcinoma
    Kumi Shoji
    Division of Nephrology and Endocrinology, University of Tokyo, Tokyo, Japan
    Am J Pathol 182:2191-203. 2013
    ..We focused on one of the novel targets, sperm-associated antigen 4 (SPAG4), whose function was unknown...
  8. ncbi Identification of a novel gene SRG4 expressed at specific stages of mouse spermatogenesis
    Xiao Wei Xing
    Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai, China
    Acta Biochim Biophys Sin (Shanghai) 36:351-9. 2004
    ..Their putative amino acid sequences have high homology with rat Spag4 (sperm antigen 4)...
  9. ncbi Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples
    Roberto Yunes
    Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA, USA
    Biocell 27:29-36. 2003
    ..Tail protein PY-phosphorylation may be related to sperm movement, especially to hyperactivated motility and its deficiency may be associated to asthenozoospermia...
  10. ncbi Expression of Tsga10 sperm tail protein in embryogenesis and neural development: from cilium to cell division
    Babak Behnam
    The Galton Laboratory, Department of Biology, University College London, London NW1 2HE, UK
    Biochem Biophys Res Commun 344:1102-10. 2006
    ..The results of the experiments in this project hypothesize the presence of Tsga10 protein wherever there is a conserved ciliary structure...
  11. ncbi [Cloning of cDNA of SRG4, a mouse spermatogenesis related gene and expression in mouse different developing stages]
    Xiao Wei Xing
    Reproduction and Stem Cell Engineering Institute, Central South University, Changsha 410078, China
    Yi Chuan Xue Bao 31:1066-71. 2004
    Spermatogenesis required lots of genes taking part in this process. In prevision studies, Odf1, Odf2, Sak57, Spag4, Spag5 were identified in mouse and rat sperm tail developing...
  12. pmc A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality
    Nicholas A Salmon
    Center for Reproductive Sciences, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, California, USA
    Genesis 44:515-22. 2006
    ..These findings are in contrast with early studies reporting Odf2 expression as testis specific and suggest that embryonic Odf2 expression plays a critical role during preimplantation development in mice...
  13. ncbi Cloning and characterization of a novel sperm tail protein, NYD-SP28
    Ying Zheng
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 10029, PR China
    Int J Mol Med 18:1119-25. 2006
    In this study, a gene coding a novel human sperm tail protein named NYD-SP28 was cloned and characterized using a complementary DNA (cDNA) microarray. Its expression was 3...
  14. doi Time-response effects of testicular gene expression profiles in Sprague-Dawley male rats treated with di(n-butyl) phthalate
    Ju Young Ryu
    College of Pharmacy, Pusan National University, Busan, South Korea
    J Toxicol Environ Health A 71:1542-9. 2008
    ..In addition, DBP significantly decreased the levels of a spermatid-specific gene (Spag4) and lactate dehydrogenase A (LDHA) mRNA after 7 d of exposure...
  15. pmc The Drosophila SUN protein Spag4 cooperates with the coiled-coil protein Yuri Gagarin to maintain association of the basal body and spermatid nucleus
    Martin P Kracklauer
    Institute for Cell and Molecular Biology and Section of Cell and Developmental Biology, The University of Texas at Austin, Austin, TX 78712, USA
    J Cell Sci 123:2763-72. 2010
    ..Here, we characterize the presumed Drosophila ortholog of the mammalian SUN protein, sperm-associated antigen 4 (Spag4, previously named Giacomo), and demonstrate that Spag4 is required for centriole and nuclear attachment during ..
  16. doi Role of phospholipase D in regulation of testicular Leydig cell hyperplasia in Sprague-Dawley rats treated with di(2-ethylhexyl) phthalate
    Young Jun Lee
    Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University, San 30, Jangjeon Dong, Geumjeung gu, Busan 609 735, South Korea
    Arch Toxicol 85:975-85. 2011
    ..DEHP significantly reduced the expression of sperm-associated antigen 4 (Spag4) and lactate dehydrogenase A (LDHA) mRNA...
  17. doi SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis
    Xian zhen Jiang
    Center for Medical Experiments, Third Xiang Ya Hospital of Central South University, Changsha, China
    DNA Cell Biol 30:875-82. 2011
    SUN domain-containing proteins belong to a novel protein family. To date, several members--SUN1, SUN2, SUN3, and SPAG4--have been identified as nuclear envelope (NE) proteins...
  18. ncbi Rat Spag5 associates in somatic cells with endoplasmic reticulum and microtubules but in spermatozoa with outer dense fibers
    Carolyn J Fitzgerald
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    Mol Reprod Dev 73:92-100. 2006
    ..Odf1, a major integral ODF protein, utilizes its leucine zipper to associate with Odf2, another major ODF protein, Spag4 which localizes to the interface between ODF and axonemal microtubule doublets, and Spag5...
  19. ncbi Sequence, expression, and chromosomal assignment of a human sperm outer dense fiber gene
    O Gastmann
    Department of Human Genetics, University of Gottingen, Germany
    Mol Reprod Dev 36:407-18. 1993
    ..The isolation of a human gene encoding a sperm tail protein may provide the ability to identify and investigate, on the molecular level, possible reasons for human male ..
  20. ncbi Human sperm associated antigen 4 (SPAG4) is a potential cancer marker
    Claire Kennedy
    Monash Institute of Reproduction and Development and The ARC Centre of Excellence in Biotechnology and Development, Monash University, 3168 Melbourne, Australia
    Cell Tissue Res 315:279-83. 2004
    ..chain reaction analyses we have determined the sequence and structure of the human sperm tail associated gene SPAG4. SPAG4 is a 12-exon gene spanning approximately 5.2 kb on chromosome 20q11.23...
  21. pmc The human genome encodes 10 alpha-crystallin-related small heat shock proteins: HspB1-10
    Guido Kappé
    Department of Biochemistry, 161 NCMLS, University of Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Cell Stress Chaperones 8:53-61. 2003
    ..alphaA-crystallin/HspB4, alphaB-crystallin/HspB5, Hsp20/HspB6, cvHsp/HspB7, H11/HspB8, and HspB9) and a sperm tail protein known since 1993 as outer dense fiber protein 1 (ODF1)...
  22. ncbi Molecular cloning and characterization of the human orthologue of the oppo 1 gene encoding a sperm tail protein
    Kouichi Kitamura
    Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Hum Reprod 9:237-43. 2003
    ..and characterization of a human orthologue of oppo 1, a mouse gene encoding a male germ-cell-specific sperm tail protein, and the organization of its genomic structure...
  23. ncbi [Cloning of cDNA of TSARG4, a human spermatogenesis related gene]
    Xiao Wei Xing
    Human Reproductive Engineering Institute, Central South University Xiangya Medical School, Changsha 410078, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:283-8. 2003
    To understand molecular mechanism of spermatogenesis, two ESTs BG720564 and AI700454, were found from SPAG4 (sperm antigen 4), a gene related to dense fiber protein of outer membrane of the human sperm and mouse spermatocytes gene ..
  24. pmc Novel RING finger protein OIP1 binds to conserved amino acid repeats in sperm tail protein ODF1
    Heather A Zarsky
    Department of Biochemistry and Molecular Biology, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1
    Biol Reprod 68:543-52. 2003
    ..leucine zipper motif of the major ODF protein ODF1, we had previously isolated interacting proteins Odf2, Spag4, and Spag5...
  25. ncbi Molecular cloning and characterization of oppo 1: a haploid germ cell-specific complementary DNA encoding sperm tail protein
    Yoshihiro Nakamura
    Department of Urology, Osaka University Medical School, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biol Reprod 67:1-7. 2002
    ..We named this newly isolated gene oppo 1, and we suggest that it plays an important role in sperm tail structure and/or sperm movement...
  26. ncbi Bypassing natural sperm selection during fertilization: the azh mutant offspring experience and the alternative of spermiogenesis in vitro
    Abraham L Kierszenbaum
    Department of Cell Biology and Anatomical Sciences, The City University of New York Medical School, 138th Street and Convent Avenue, J 903, New York, NY 10031, USA
    Mol Cell Endocrinol 187:133-8. 2002
    ..N-arginine convertase (both sorted to the developing spermatid tail), a spermatid perinuclear RNA binding protein, Spag4, an Odf1-binding protein, and type 4 cAMP-specific phosphodiesterase D...
  27. ncbi Molecular cloning and characterization of a complementary DNA encoding sperm tail protein SHIPPO 1
    Carlos Egydio de Carvalho
    Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka 565 0871, Japan
    Biol Reprod 66:785-95. 2002
    ..SHIPPO 1 in sperm is resistant to treatment with nonionic detergents and coextracted with the cytoskeletal core proteins of the mouse sperm tail...
  28. ncbi Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1
    X Shao
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Mol Reprod Dev 59:410-6. 2001
    ..Interestingly, these motifs are present in Deepest. These results highlight the importance of the leucine zipper in sperm tail protein interactions. Mol. Reprod. Dev. 59: 410-416, 2001.
  29. ncbi Spermatid manchette: plugging proteins to zero into the sperm tail
    A L Kierszenbaum
    Department of Cell Biology and Anatomical Sciences, The City University of New York Medical School, 138th Street and Convent Avenue, New York, NY 10031, USA
    Mol Reprod Dev 59:347-9. 2001
    ..that three keratins-Sak57, 0df1 and 0df2-and other proteins (the 26S proteasome and the 0df1-binding protein Spag4) are temporarily stored in the manchette before being sorted to the developing sperm tail...
  30. ncbi A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2
    H Tarnasky
    Department of Medical Biochemistry, Southern Alberta Cancer Research Centre, University of Calgary, Calgary, Alberta Canada
    Cytogenet Cell Genet 81:65-7. 1998
    The product of the spermatid-specific rat SPAG4 gene interacts specifically with the major 27-kDa outer dense fiber protein ODF27 in elongating spermatids...

Research Grants1

  1. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..