SLC9A3R1

Summary

Gene Symbol: SLC9A3R1
Description: SLC9A3 regulator 1
Alias: EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, ezrin-radixin-moesin binding phosphoprotein-50, regulatory cofactor of Na(+)/H(+) exchanger, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1, solute carrier family 9 isoform A3 regulatory factor 1, solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Species: human

Top Publications

  1. pmc A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins
    R A Hall
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 95:8496-501. 1998
  2. pmc A regulated complex of the scaffolding proteins PDZK1 and EBP50 with ezrin contribute to microvillar organization
    David P LaLonde
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 21:1519-29. 2010
  3. doi Loss of EBP50 stimulates EGFR activity to induce EMT phenotypic features in biliary cancer cells
    A Claperon
    INSERM, UMRS_938, Paris, France
    Oncogene 31:1376-88. 2012
  4. doi EBP50 exerts tumor suppressor activity by promoting cell apoptosis and retarding extracellular signal-regulated kinase activity
    Jun Fang Zheng
    Department of Biochemistry and Molecular Biology, Capital Medical University, No 10 Xitoutiao, You An Men, 100069, Beijing, People s Republic of China
    Amino Acids 38:1261-8. 2010
  5. ncbi The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain
    Casey M Finnerty
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    J Cell Sci 117:1547-52. 2004
  6. ncbi Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer
    J Song
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Histopathology 51:40-53. 2007
  7. pmc Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction
    V Raghuram
    Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104 6100, USA
    Proc Natl Acad Sci U S A 98:1300-5. 2001
  8. ncbi Estrogen receptor inducibility of the human Na+/H+ exchanger regulatory factor/ezrin-radixin-moesin binding protein 50 (NHE-RF/EBP50) gene involving multiple half-estrogen response elements
    Tracy R Ediger
    Department of Cell and Structural Biology, University of Illinois and College of Medicine, Urbana, Illinois 61801 3704, USA
    Mol Endocrinol 16:1828-39. 2002
  9. ncbi A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated with susceptibility to psoriasis
    Cynthia Helms
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nat Genet 35:349-56. 2003
  10. ncbi A NHERF binding site links the betaPDGFR to the cytoskeleton and regulates cell spreading and migration
    Marianne F James
    Molecular Neurogenetics Unit, Center for Human Genetic Research, Massachusetts General Hospital, Building 149, 13th Street, Charlestown, MA 02129, USA
    J Cell Sci 117:2951-61. 2004

Research Grants

  1. NHERF-1 and dopamine-mediated regulation of renal phosphate transport.
    Edward J Weinman; Fiscal Year: 2013
  2. Pradeep K Dudeja; Fiscal Year: 2016
  3. Analysis of PTH and Dopamine Receptor Signaling in Proximal Tubules
    Eleanor D Lederer; Fiscal Year: 2013
  4. NHERF-1 and PTH regulation of the renal transport of phosphate.
    Edward J Weinman; Fiscal Year: 2012
  5. PTH1R SIGNALING BY CYTOPLASMIC BINDING PARTNERS
    WILLIAM SNEDDON; Fiscal Year: 2005
  6. NHERFs Specify PTH Receptor Signaling
    Harald Juppner; Fiscal Year: 2005
  7. Hassane Amlal; Fiscal Year: 2014
  8. Peter A Friedman; Fiscal Year: 2014
  9. Salman Azhar; Fiscal Year: 2014
  10. Christopher S Wilcox; Fiscal Year: 2014

Detail Information

Publications179 found, 100 shown here

  1. pmc A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins
    R A Hall
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 95:8496-501. 1998
    The Na+/H+ exchanger regulatory factor (NHERF) binds to the tail of the beta2-adrenergic receptor and plays a role in adrenergic regulation of Na+/H+ exchange...
  2. pmc A regulated complex of the scaffolding proteins PDZK1 and EBP50 with ezrin contribute to microvillar organization
    David P LaLonde
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 21:1519-29. 2010
    PDZK1 and ezrin, radixin, moesin binding phosphoprotein 50 kDa (EBP50) are postsynaptic density 95/disc-large/zona occludens (PDZ)-domain-containing scaffolding proteins found in the apical microvilli of polarized epithelial cells...
  3. doi Loss of EBP50 stimulates EGFR activity to induce EMT phenotypic features in biliary cancer cells
    A Claperon
    INSERM, UMRS_938, Paris, France
    Oncogene 31:1376-88. 2012
    ..The scaffold protein ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is highly expressed at the plasma membrane of normal biliary epithelial cells and binds epidermal growth factor ..
  4. doi EBP50 exerts tumor suppressor activity by promoting cell apoptosis and retarding extracellular signal-regulated kinase activity
    Jun Fang Zheng
    Department of Biochemistry and Molecular Biology, Capital Medical University, No 10 Xitoutiao, You An Men, 100069, Beijing, People s Republic of China
    Amino Acids 38:1261-8. 2010
    The expression of Ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) and the intragenic mutation of the ebp50 gene have been reported to correlate with human breast cancer development, but the exact impacts on breast cancer ..
  5. ncbi The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain
    Casey M Finnerty
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    J Cell Sci 117:1547-52. 2004
    ..crosslinking proteins that, upon activation, bind the scaffolding protein ERM-phosphoprotein of 50 kDa (EBP50). Here we report a 3...
  6. ncbi Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer
    J Song
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Histopathology 51:40-53. 2007
    Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a post synaptic density-95/Disk-large/ZO-1 homologous domain-containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton and plays an ..
  7. pmc Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction
    V Raghuram
    Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104 6100, USA
    Proc Natl Acad Sci U S A 98:1300-5. 2001
    ..The Na(+)/H(+) exchanger regulatory factor (NHERF) binds to the cytoplasmic tail of CFTR through either of its two PDZ (PDZ1 and PDZ2) domains...
  8. ncbi Estrogen receptor inducibility of the human Na+/H+ exchanger regulatory factor/ezrin-radixin-moesin binding protein 50 (NHE-RF/EBP50) gene involving multiple half-estrogen response elements
    Tracy R Ediger
    Department of Cell and Structural Biology, University of Illinois and College of Medicine, Urbana, Illinois 61801 3704, USA
    Mol Endocrinol 16:1828-39. 2002
    ..Our findings highlight a paradigm for gene regulation via numerous half-ERE sites that expands the range of modes by which DNA recognition sites mediate the actions of this nuclear receptor...
  9. ncbi A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated with susceptibility to psoriasis
    Cynthia Helms
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nat Genet 35:349-56. 2003
    ..Associated single-nucleotide polymorphisms (SNPs) in the proximal peak lie in or near SLC9A3R1 (also called EBP50 and NHERF1) and NAT9, a new member of the N-acetyltransferase family...
  10. ncbi A NHERF binding site links the betaPDGFR to the cytoskeleton and regulates cell spreading and migration
    Marianne F James
    Molecular Neurogenetics Unit, Center for Human Genetic Research, Massachusetts General Hospital, Building 149, 13th Street, Charlestown, MA 02129, USA
    J Cell Sci 117:2951-61. 2004
    The Na(+)/H(+) exchanger regulatory factor, NHERF, is a multifunctional adapter protein involved in a wide range of physiological activities...
  11. pmc Analysis of polymorphisms in 16 genes in type 1 diabetes that have been associated with other immune-mediated diseases
    Deborah J Smyth
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Addenbrooke s Hospital, Hills Rd, Cambridge, CB2 2XY, UK
    BMC Med Genet 7:20. 2006
    ..Therefore, we tested the hypothesis that alleles of susceptibility genes previously associated with other immune-mediated diseases might perturb immune homeostasis, and hence also associate with predisposition to T1D...
  12. pmc NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands
    Fabiana C Morales
    Department of Neuro Oncology, M D Anderson Cancer Center, The University of Texas, 6767 Bertner Avenue, Houston, TX 77030, USA
    Mol Cell Biol 27:2527-37. 2007
    ..We describe here an intramolecular conformation of NHERF1/EBP50 (ERM-binding phosphoprotein 50) in which the C-terminal EB region binds to the PDZ2 domain...
  13. pmc Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1
    Jianquan Li
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Mol Biol 392:166-80. 2009
    ..The scaffolding protein Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) assembles macromolecular signaling complexes and regulates the macromolecular assembly, localization, and ..
  14. pmc Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger
    E J Weinman
    Department of Medicine, University of California, Los Angeles School of Medicine, USA
    J Clin Invest 95:2143-9. 1995
    ..Reverse transcription/PCR studies in the kidney indicate the presence of mRNA for NHE-RF in several distinct nephron segments including the proximal tubule...
  15. pmc Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family
    D Reczek
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 139:169-79. 1997
    ..We refer to these polypeptides as ERM-binding phosphoprotein 50 (EBP50)...
  16. ncbi The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange
    R A Hall
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 392:626-30. 1998
    ..direct agonist-promoted association of the beta2-adrenergic receptor with the Na+/H+ exchanger regulatory factor (NHERF), a protein that regulates the activity of the Na+/H+ exchanger type 3 (NHE3)...
  17. ncbi Peptide binding consensus of the NHE-RF-PDZ1 domain matches the C-terminal sequence of cystic fibrosis transmembrane conductance regulator (CFTR)
    S Wang
    Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    FEBS Lett 427:103-8. 1998
    ..The specific and tight association suggests a potential regulatory role of NHE-RF in cystic fibrosis transmembrane conductance regulator (CFTR) function...
  18. ncbi An apical PDZ protein anchors the cystic fibrosis transmembrane conductance regulator to the cytoskeleton
    D B Short
    Department of Physiology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 273:19797-801. 1998
    ..Here we report that CFTR interacts with the recently discovered protein, EBP50 (ERM-binding phosphoprotein 50)...
  19. ncbi G protein-coupled receptor kinase 6A phosphorylates the Na(+)/H(+) exchanger regulatory factor via a PDZ domain-mediated interaction
    R A Hall
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 274:24328-34. 1999
    The Na(+)/H(+) exchanger regulatory factor (NHERF) is constitutively phosphorylated in cells, but the site(s) of this phosphorylation and the kinase(s) responsible for it have not been identified...
  20. ncbi A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor
    T T Cao
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Nature 401:286-90. 1999
    ..The phosphoprotein EBP50 (for ezrinradixin-moesin(ERM)-binding phosphoprotein-50) binds to the cytoplasmic tail of the beta2-adrenergic ..
  21. pmc Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50
    P J Mohler
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 147:879-90. 1999
    ..of the cystic fibrosis transmembrane conductance regulator associates with the submembranous scaffolding protein EBP50 (ERM-binding phosphoprotein 50 kD; also called Na(+)/H(+) exchanger regulatory factor)...
  22. ncbi The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells
    S Breton
    Renal Unit and Program in Membrane Biology and the Molecular Neurogenetics Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 275:18219-24. 2000
    ..The colocalization of NHE-RF and H(+)ATPase in B- but not A-intercalated cells suggests a role in generating, maintaining, or modulating the variable H(+)ATPase polarity that characterizes the B-cell phenotype...
  23. ncbi Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF
    Y Tang
    Neurobiotechnology Center and Department of Neuroscience, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 275:37559-64. 2000
    ..show that murine Trp4 and Trp5, as well as phospholipase C beta1 and beta2 interact with the first PDZ domain of NHERF, regulatory factor of the Na(+)/H(+) exchanger...
  24. pmc Platelet-derived growth factor receptor association with Na(+)/H(+) exchanger regulatory factor potentiates receptor activity
    S Maudsley
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell Biol 20:8352-63. 2000
    ..We show here that the Na(+)/H(+) exchanger regulatory factor (NHERF; also known as EBP50), a protein not previously known to interact with the PDGFR, binds to the PDGFR carboxyl ..
  25. ncbi Interaction of the type IIa Na/Pi cotransporter with PDZ proteins
    S M Gisler
    Institute of Physiology, Veterinary Biochemistry, and Anatomy, University of Zurich Irchel, CH 8057 Zurich, Switzerland
    J Biol Chem 276:9206-13. 2001
    ..found belonged to proteins with multiple PDZ modules and were either identical/related to PDZK1 or identical to NHERF-1...
  26. pmc Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP
    D Reczek
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 153:191-206. 2001
    The cortical scaffolding proteins EBP50 (ERM-binding phosphoprotein-50) and E3KARP (NHE3 kinase A regulatory protein) contain two PDZ (PSD-95/DlgA/ZO-1-like) domains followed by a COOH-terminal sequence that binds to active ERM family ..
  27. ncbi Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator
    S Karthikeyan
    Molecular Medicine Laboratory and Macromolecular Crystallography Unit, Division of Experimental Medicine, Harvard Institutes of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:19683-6. 2001
    The PDZ1 domain of the Na(+)/H(+) exchanger regulatory factor (NHERF) binds with nanomolar affinity to the carboxyl-terminal sequence QDTRL of the cystic fibrosis transmembrane conductance regulator (CFTR) and plays a central role in the ..
  28. ncbi Crystal structure of the PDZ1 domain of human Na(+)/H(+) exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains
    S Karthikeyan
    Molecular Medicine Laboratory and Macromolecular Crystallography Unit, Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
    J Mol Biol 308:963-73. 2001
    The Na(+)/H(+) exchanger regulatory factor (NHERF; also known as EBP50) contains two PDZ domains that mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes...
  29. ncbi Phosphorylation and cell cycle-dependent regulation of Na+/H+ exchanger regulatory factor-1 by Cdc2 kinase
    J He
    Department of Pharmacology, Rollins Research Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 276:41559-65. 2001
    Na(+)/H(+) exchanger regulatory factor (NHERF)-1 is a PDZ domain-containing adaptor protein known to bind to various receptors, channels, cytoskeletal elements, and cytoplasmic signaling proteins...
  30. ncbi Structural determinants of the Na+/H+ exchanger regulatory factor interaction with the beta 2 adrenergic and platelet-derived growth factor receptors
    Subramanian Karthikeyan
    Molecular Medicine Laboratory and Macromolecular Crystallography Unit, Division of Experimental Medicine, Harvard Institutes of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:18973-8. 2002
    The Na(+)/H(+) exchanger regulatory factor (NHERF) binds through its PDZ1 domain to the carboxyl-terminal sequences NDSLL and EDSFL of the beta(2) adrenergic receptor (beta(2)AR) and platelet-derived growth factor receptor, respectively, ..
  31. ncbi Distinct cell type-specific expression of scaffolding proteins EBP50 and E3KARP: EBP50 is generally expressed with ezrin in specific epithelia, whereas E3KARP is not
    Janet Ingraffea
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Eur J Cell Biol 81:61-8. 2002
    ..b>EBP50 and E3KARP are two related scaffolding proteins that bind to the activated form of ERM proteins in vitro, and ..
  32. ncbi Na(+)/H(+ ) exchanger regulatory factor 2 directs parathyroid hormone 1 receptor signalling
    Matthew J Mahon
    Endocrine Unit, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 417:858-61. 2002
    ..Here we show that the PTH1R binds to Na(+)/H(+) exchanger regulatory factors (NHERF) 1 and 2 through a PDZ-domain interaction in vitro and in PTH target cells...
  33. ncbi EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma
    Tatsuhiro Shibata
    Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
    Hepatology 38:178-86. 2003
    ..By protein interaction screening, we identified EBP50, a cytoplasmic protein with 2 PDZ domains, as a beta-catenin-associating molecule...
  34. ncbi Ca2+-dependent inhibition of NHE3 requires PKC alpha which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexes
    Whaseon Lee-Kwon
    Johns Hopkins Univ School of Medicine, 925 Ross Research Bldg, 720 Rutland Ave, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 285:C1527-36. 2003
    ..These results suggest that PKCalpha is not necessary for the Ca2+-dependent formation of the NHE3 plasma membrane complex, although it is necessary for decreasing the membrane amounts of NHE3, probably by stimulating NHE3 endocytosis...
  35. ncbi A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Biochemistry 42:12662-8. 2003
    b>NHERF-1, a protein adapter containing two tandem PDZ domains, was first identified as an essential cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP...
  36. ncbi kappa Opioid receptor interacts with Na(+)/H(+)-exchanger regulatory factor-1/Ezrin-radixin-moesin-binding phosphoprotein-50 (NHERF-1/EBP50) to stimulate Na(+)/H(+) exchange independent of G(i)/G(o) proteins
    Peng Huang
    Department of Pharmacology and Center for Substance Abuse Research, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 279:25002-9. 2004
    We previously showed that Na(+)/H(+)-exchanger regulatory factor-1/Ezrin-radixin-moesin-binding phosphoprotein-50 (NHERF-1/EBP50) co-immunoprecipitated with the human kappa opioid receptor (hKOR) and that its overexpression blocked the ..
  37. ncbi NHERF (Na+/H+ exchanger regulatory factor) gene mutations in human breast cancer
    Jia Le Dai
    Department of Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 23:8681-7. 2004
    ..The screening yielded NHERF (Na+/H+ exchanger regulatory factor, also known as NHERF1 or EBP-50) that binds to the interdomain B of SYK...
  38. ncbi PDZK1 directly regulates the function of organic cation/carnitine transporter OCTN2
    Yukio Kato
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Ishikawa 920 1192, Japan
    Mol Pharmacol 67:734-43. 2005
    ..The present findings are the first to identify PDZK1 as a functional regulator of OCTN2 through direct interaction with the C terminus...
  39. ncbi Ezrin controls the macromolecular complexes formed between an adapter protein Na+/H+ exchanger regulatory factor and the cystic fibrosis transmembrane conductance regulator
    Jianquan Li
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Biol Chem 280:37634-43. 2005
    Na(+)/H(+) exchanger regulatory factor (NHERF) is an adapter protein that is responsible for organizing a number of cell receptors and channels...
  40. ncbi Modulation of renal apical organic anion transporter 4 function by two PDZ domain-containing proteins
    Hiroki Miyazaki
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka shi, Tokyo, Japan
    J Am Soc Nephrol 16:3498-506. 2005
    ..Here, two multivalent PDZ (PSD-95/Discs Large/ZO-1) proteins PDZK1 and NHERF1 were examined as interactors of OAT4 by a yeast two-hybrid assay...
  41. pmc PTEN tumor suppressor associates with NHERF proteins to attenuate PDGF receptor signaling
    Yoko Takahashi
    Department of Neuro Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    EMBO J 25:910-20. 2006
    ..We show here that PTEN interacts directly with the NHERF1 and NHERF2 (Na+/H+ exchanger regulatory factor) homologous adaptor proteins through the PDZ motif of PTEN and the ..
  42. ncbi Structural basis for NHERF recognition by ERM proteins
    Shin ichi Terawaki
    Structural Biology Laboratory, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Structure 14:777-89. 2006
    The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein involved in the anchoring of ion channels and receptors to the actin cytoskeleton through binding to ERM (ezrin/radixin/moesin) proteins...
  43. pmc Suppression of breast cancer cell growth by Na+/H+ exchanger regulatory factor 1 (NHERF1)
    Yong Pan
    Department of Molecular Pathology, The University of Texas M D Anderson Cancer Center, Houston, TX 77054, USA
    Breast Cancer Res 8:R63. 2006
    Na+/H+ exchanger regulatory factor 1 (NHERF1, also known as EBP50 or NHERF) is a putative tumour suppressor gene in human breast cancer. Located at 17q25.1, NHERF1 is frequently targeted during breast tumourigenesis...
  44. ncbi Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells
    Fanfan Zhou
    Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854, USA
    Pharm Res 25:475-80. 2008
    ..To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells...
  45. ncbi Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly
    Jianquan Li
    Basic Science Division and Population Science Division, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    J Biol Chem 282:27086-99. 2007
    ..kinase C (PKC) phosphorylation influences the scaffolding protein Na(+)/H(+) exchanger regulatory factor 1 (NHERF) to assemble protein complexes of cystic fibrosis transmembrane conductance regulator (CFTR), a chloride ion ..
  46. ncbi Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets
    James W Voltz
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 282:33879-87. 2007
    b>NHERF-1 (Na(+)-H(+) exchanger regulatory factor 1, also known as EBP50 ezrin-binding protein of 50 kDa) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain...
  47. pmc The relative binding affinities of PDZ partners for CFTR: a biochemical basis for efficient endocytic recycling
    Patrick R Cushing
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Biochemistry 47:10084-98. 2008
    ..competition between CAL and other PDZ proteins including Na (+)/H (+) exchanger-3 regulatory factors 1 and 2 (NHERF1 and NHERF2), which functionally stabilize cell-surface CFTR...
  48. doi NHERF1 mutations and responsiveness of renal parathyroid hormone
    Zoubida Karim
    INSERM Unité 845, Universite Paris Descartes, Faculte de Medecine, Paris, France
    N Engl J Med 359:1128-35. 2008
    ..Data from animal models suggest that sodium-hydrogen exchanger regulatory factor 1 (NHERF1) controls renal phosphate transport...
  49. pmc The scaffold protein PDZK1 undergoes a head-to-tail intramolecular association that negatively regulates its interaction with EBP50
    David P LaLonde
    Weill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 48:2261-71. 2009
    ..A head-to-tail association has also been reported for EBP50/NHERF1, a two-PDZ domain member of the same scaffolding protein family as PDZK1, and shown to regulate binding of target ..
  50. pmc NHERF1 regulates parathyroid hormone receptor desensitization: interference with beta-arrestin binding
    Bin Wang
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Mol Pharmacol 75:1189-97. 2009
    ..The Na(+)/H(+) exchange regulatory factor 1 (NHERF1) is a cytoplasmic adapter protein that regulates trafficking and signaling of several G protein-coupled receptors (..
  51. pmc Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation
    Laura Fouassier
    INSERM, UMR_S 893, CdR Saint Antoine, Faculte de Medecine Pierre et Marie Curie, site Saint Antoine, 27, rue Chaligny, 75571 Paris Cedex 12, France
    Am J Pathol 174:869-80. 2009
    Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) anchors and regulates apical membrane proteins in epithelia. EBP50 is inducible by estrogen and may affect cell proliferation, although this latter function remains unclear...
  52. pmc NHERF-1: modulator of glioblastoma cell migration and invasion
    Kerri L Kislin
    Cancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZ 85004, USA
    Neoplasia 11:377-87. 2009
    ..Na(+)/H(+) exchanger regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein, recruiting cytoplasmic signaling proteins and membrane receptors/..
  53. doi Biological role of NHERF1 protein expression in breast cancer
    Anita Mangia
    Clinical Experimental Oncology Laboratory, National Cancer Institute, Bari, Italy
    Histopathology 55:600-8. 2009
    To determine the role of Na+/H+ exchanger regulatory factor (NHERF1) in breast cancerogenesis and progression.
  54. pmc Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits
    Bin Wang
    Laboratory for G Protein coupled Receptor Biology, Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 285:26976-86. 2010
    The Na/H exchanger regulatory factors, NHERF1 and NHERF2, are adapter proteins involved in targeting and assembly of protein complexes. The parathyroid hormone receptor (PTHR) interacts with both NHERF1 and NHERF2...
  55. pmc Cooperativity between the phosphorylation of Thr95 and Ser77 of NHERF-1 in the hormonal regulation of renal phosphate transport
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 285:25134-8. 2010
    The phosphorylation of the sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) plays a key role in the regulation of renal phosphate transport by parathyroid hormone (PTH) and dopamine...
  56. pmc Loss of PTEN binding adapter protein NHERF1 from plasma membrane in glioblastoma contributes to PTEN inactivation
    Jennifer R Molina
    Department of Neuro Oncology and Pathology, The University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Cancer Res 70:6697-703. 2010
    ..The PTEN binding adapter protein NHERF1/EBP50 is overexpressed in GBM but its effects on tumorigenesis have yet to be determined...
  57. pmc Na+/H+ exchanger regulatory factor-1 is involved in chemokine receptor homodimer CCR5 internalization and signal transduction but does not affect CXCR4 homodimer or CXCR4-CCR5 heterodimer
    Maha M Hammad
    Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
    J Biol Chem 285:34653-64. 2010
    ..We identified a receptor adaptor interacting specifically with one receptor complex but not others. NHERF1, an adaptor known for its role in desensitization, internalization, and regulation of the ERK signaling cascade ..
  58. pmc Direct interaction between NHERF1 and Frizzled regulates β-catenin signaling
    D S Wheeler
    Laboratory for GPCR Biology, Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Oncogene 30:32-42. 2011
    ..We show here that the adaptor protein Na(+)/H(+) exchange regulatory factor1 (NHERF1), a protein abundantly expressed in normal mammary epithelium, regulates Wnt signaling, maintaining low levels of ..
  59. pmc The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner
    Damien Garbett
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Weill Hall, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 191:397-413. 2010
    ..A potential regulator is EBP50/NHERF1 (ERM-binding phosphoprotein of 50 kD/Na(+)-H(+) exchanger regulatory factor), a microvillar scaffolding protein ..
  60. pmc NHERF1/EBP50 is a new marker in colorectal cancer
    Yuho Hayashi
    Department of Neuro Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Neoplasia 12:1013-22. 2010
    ..We report here that Na(+)/H(+) exchanger 3 regulating factor 1 (NHERF1)/EBP50, an adaptor molecule that interacts with β-catenin, undergoes successive alterations during the colorectal ..
  61. pmc NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity
    Rafiquel Sarker
    Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ School of Medicine, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 300:C771-82. 2011
    ..Most studies of NHE3 regulation have been in cell models in which NHERF1 and/or NHERF2 were overexpressed...
  62. doi Gene expression profiling of luminal B breast cancers reveals NHERF1 as a new marker of endocrine resistance
    Thomas Karn
    Department of Obstetrics and Gynecology, J W Goethe University, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    Breast Cancer Res Treat 130:409-20. 2011
    ..The clinical relevance of the scaffold protein NHERF1 identified by this strategy was assessed in a total of 3,030 breast cancers...
  63. doi Human epidermal growth factor receptor 2, Na+/H+ exchanger regulatory factor 1, and breast cancer susceptibility gene-1 as new biomarkers for familial breast cancers
    Annita Mangia
    Clinical Experimental Oncology Laboratory, National Cancer Centre, Viale Orazio Flacco, 65 70124 Bari, Italy
    Hum Pathol 42:1589-95. 2011
    ....
  64. pmc E6 and E7 from human papillomavirus type 16 cooperate to target the PDZ protein Na/H exchange regulatory factor 1
    Rosita Accardi
    Infections and Cancer Biology Group, International Agency for Research on Cancer, World Health Organization, France
    J Virol 85:8208-16. 2011
    ..a novel interaction of HPV type 16 (HPV16) E6 with a PDZ protein, Na(+)/H(+) exchange regulatory factor 1 (NHERF-1), which is involved in a number of cellular processes, including signaling and transformation...
  65. doi Na+/H+ exchanger regulatory factor 1 expression levels in blood and tissue predict breast tumour clinical behaviour
    Antonia Bellizzi
    Clinical Experimental Oncology Laboratory, National Cancer Centre, University of Bari, Bari, Italy
    Histopathology 58:1086-95. 2011
    Several studies have demonstrated that Na(+) /H(+) exchanger regulatory factor 1 (NHERF1) protein, which is overexpressed and heterogeneously distributed in different stages of breast cancer, could be used as a tumour marker for prognosis ..
  66. pmc PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma
    J R Molina
    Department of Neuro Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 31:1264-74. 2012
    ..pathway in glioblastoma within a tumor suppressor network that includes Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) and pleckstrin-homology domain leucine-rich repeat protein phosphatases 1 (PHLPP1)...
  67. pmc Dynamic Na+-H+ exchanger regulatory factor-1 association and dissociation regulate parathyroid hormone receptor trafficking at membrane microdomains
    Juan A Ardura
    Laboratory for G Protein coupled Receptor Biology, Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 286:35020-9. 2011
    Na/H exchanger regulatory factor-1 (NHERF1) is a cytoplasmic PDZ (postsynaptic density 95/disc large/zona occludens) protein that assembles macromolecular complexes and determines the localization, trafficking, and signaling of select G ..
  68. doi Overexpression of nuclear NHERF1 in advanced colorectal cancer: association with hypoxic microenvironment and tumor invasive phenotype
    Andrea Malfettone
    Functional Biomorphology Laboratory, National Cancer Centre Giovanni Paolo II, Bari, Italy
    Exp Mol Pathol 92:296-303. 2012
    ..The present study investigates protein expression of NHERF1, HIF-1α and TWIST1 and their relationship in distant normal mucosa (DNM), tumor (T) and adjacent normal mucosa (..
  69. pmc NHERF1 acts as a molecular switch to program metastatic behavior and organotropism via its PDZ domains
    Rosa Angela Cardone
    Department of Bioscience, Biotechnology and Pharmacological Sciences, University of Bari, Bari, Italy
    Mol Biol Cell 23:2028-40. 2012
    ..Na(+)/H(+) exchanger regulatory factor (NHERF1) is a molecular pathway organizer, PDZ-domain protein that recruits membrane, cytoplasmic, and cytoskeletal ..
  70. pmc PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli
    Damien Garbett
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 198:195-203. 2012
    ..The microvillar scaffolding protein EBP50 (ERM-binding phosphoprotein of 50 kD), consisting of two PDZ domains and an ezrin-binding site, retains specific ..
  71. pmc Ligand-induced dynamic changes in extended PDZ domains from NHERF1
    Shibani Bhattacharya
    New York Structural Biology Center, 89 Convent Avenue, New York, NY 10027, USA
    J Mol Biol 425:2509-28. 2013
    The multi-domain scaffolding protein NHERF1 modulates the assembly and intracellular trafficking of various transmembrane receptors and ion-transport proteins...
  72. pmc cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein
    C H Yun
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 94:3010-5. 1997
    ..We have identified two regulatory proteins, E3KARP and NHERF, that interact with NHE3 to enable cAMP to inhibit NHE3...
  73. ncbi NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins
    A Murthy
    Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown 02129, USA
    J Biol Chem 273:1273-6. 1998
    ....
  74. pmc A kinase-regulated mechanism controls CFTR channel gating by disrupting bivalent PDZ domain interactions
    Viswanathan Raghuram
    Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104 6085, USA
    Proc Natl Acad Sci U S A 100:9620-5. 2003
    ..The Na+/H+ exchanger regulatory factor (NHERF) binds to CFTR and increases its open probability (Po)...
  75. pmc The NHERF1 PDZ2 domain regulates PKA-RhoA-p38-mediated NHE1 activation and invasion in breast tumor cells
    Rosa A Cardone
    Department of General and Environmental Physiology, University of Bari, 70126 Bari, Italy
    Mol Biol Cell 18:1768-80. 2007
    ..Na(+)/H(+) exchanger regulatory factor (NHERF1) is a postsynaptic density 95/disc-large/zona occludens (PDZ) domain-containing protein that recruits membrane ..
  76. ncbi NHERF1 regulates parathyroid hormone receptor membrane retention without affecting recycling
    Bin Wang
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 282:36214-22. 2007
    Na/H exchange regulatory factor-1 (NHERF1) is a PDZ protein that regulates trafficking of several G protein-coupled receptors...
  77. ncbi Molecular mechanisms of estrogen action: selective ligands and receptor pharmacology
    B S Katzenellenbogen
    Department of Molecular and Integrative Physiology, University of Illinois and College of Medicine, 524 Burrill Hall, 407 S Goodwin Avenue, 61801 3704, Urbana, IL, USA
    J Steroid Biochem Mol Biol 74:279-85. 2000
    ..cell cytoarchitectural changes including NHE-RF, the sodium hydrogen exchanger regulatory factor, also known as EBP50. NHE-RF/EBP50 is enriched in microvilli, and may serve as a scaffold adaptor protein in regulating early changes ..
  78. ncbi Parathyroid hormone regulation of NA+,K+-ATPase requires the PDZ 1 domain of sodium hydrogen exchanger regulatory factor-1 in opossum kidney cells
    Syed Jalal Khundmiri
    Department of Medicine, University of Louisville, Kidney Disease Program, Louisville, KY 40202, USA
    J Am Soc Nephrol 16:2598-607. 2005
    It was demonstrated that expression of murine sodium hydrogen exchanger regulatory factor (NHERF-1) lacking the ezrin-binding domain blocks parathyroid hormone (PTH) regulation of Na+,K+-ATPase in opossum kidney (OK) cells...
  79. doi Ezrin promotes functional expression and parathyroid hormone-mediated regulation of the sodium-phosphate cotransporter 2a in LLC-PK1 cells
    Matthew J Mahon
    Department of Medicine, Harvard Medical School and Endocrine Unit, Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 294:F667-75. 2008
    ..membrane expression of NPT2a is dependent on interactions with the sodium-hydrogen exchanger regulatory factor 1 (NHERF-1)...
  80. doi Role of NHERF and scaffolding proteins in proximal tubule transport
    Rochelle Cunningham
    Division of Nephrology, Department of Medicine, University of Maryland, School of Medicine, Room N3W143, UHM, 22 South Greene Street, Baltimore, MD 21201, USA
    Urol Res 38:257-62. 2010
    ..The NHERF (sodium hydrogen exchanger regulatory factor) proteins are PDZ domain-containing proteins that play an important ..
  81. ncbi Molecular effects of volume expansion on the renal sodium phosphate cotransporter
    Jules B Puschett
    Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana, USA
    Am J Med Sci 326:1-8. 2003
    ..In recent experiments, Sprague-Dawley rats were subjected to the same short term VE. For both control and VE animals, brush border membrane vesicles (BBMV) were obtained...
  82. pmc PTH transiently increases the percent mobile fraction of Npt2a in OK cells as determined by FRAP
    Edward J Weinman
    Dept of Medicine, Div of Nephrology, Univ of Maryland School of Medicine, Baltimore, MD 21202, USA
    Am J Physiol Renal Physiol 297:F1560-5. 2009
    ..2a (Npt2a) binds to a number of PDZ adaptor proteins including sodium-hydrogen exchanger regulatory factor-1 (NHERF-1), which regulates its retention in the apical membrane of renal proximal tubule cells and the response to ..
  83. doi Synthesis and screening of support-bound combinatorial peptide libraries with free C-termini: determination of the sequence specificity of PDZ domains
    Sang Hoon Joo
    Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 47:3061-72. 2008
    ..density-95/discs large/zona occluden-1 (PDZ) domains of sodium-hydrogen exchanger regulatory factor-1 (NHERF1) and channel-interacting PDZ domain protein (CIPP)...
  84. ncbi Sodium-hydrogen exchanger regulatory factor-1 interacts with mouse urate transporter 1 to regulate renal proximal tubule uric acid transport
    Rochelle Cunningham
    Department of Medicine, University of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
    J Am Soc Nephrol 18:1419-25. 2007
    Sodium-hydrogen exchanger regulatory factor-1-deficient (NHERF-1(-/-)) mice demonstrate increases in the urinary excretion of phosphate, calcium, and uric acid associated with interstitial deposition of calcium in the papilla of the ..
  85. ncbi Expression of NHERF-1, NHERF-2, PDGFR-alpha, and PDGFR-beta in normal human kidneys and in renal transplant rejection
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA
    Pathobiology 70:314-23. 2002
    Sodium-hydrogen exchanger regulatory factor-1 and -2 (NHERF-1 and NHERF-2) are adaptor proteins that regulate renal electrolyte transport and interact with the platelet-derived growth factor receptors (PDGFR)...
  86. doi Novel regulatory function for NHERF-1 in Npt2a transcription
    Syed Jalal Khundmiri
    Department of Medicine, Univ of Louisville, Kidney Disease Program, 570 S Preston St, Suite 102, Louisville, KY 40202, USA
    Am J Physiol Renal Physiol 294:F840-9. 2008
    Several lines of evidence show that sodium/hydrogen exchanger regulatory factor 1 (NHERF-1) regulates the expression and activity of the type IIa sodium-dependent phosphate transporter (Npt2a) in renal proximal tubules...
  87. pmc The parathyroid hormone 1 receptor directly binds to the FERM domain of ezrin, an interaction that supports apical receptor localization and signaling in LLC-PK1 cells
    Matthew J Mahon
    Endocrine Unit, Massachusetts General Hospital, 50 Blossom Street, Their 10, Boston, Massachusetts 02114, USA
    Mol Endocrinol 23:1691-701. 2009
    ..a psd-95, discs large, ZO-1 domain interaction motif that binds the sodium-hydrogen exchanger regulatory factor 1 (NHERF-1), a renal tubule scaffold protein...
  88. pmc Parathyroid hormone inhibits renal phosphate transport by phosphorylation of serine 77 of sodium-hydrogen exchanger regulatory factor-1
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Clin Invest 117:3412-20. 2007
    ..77 (S77) of the first PDZ domain of the Npt2a-binding protein sodium-hydrogen exchanger regulatory factor-1 (NHERF-1). Using recombinant polypeptides representing PDZ I, S77 of NHERF-1 is phosphorylated by PKC but not PKA...
  89. pmc Localizations of visual cycle components in retinal pigment epithelium
    Jing Huang
    Department of Ophthalmology, University of Washington, School of Medicine, Seattle, WA, USA
    Mol Vis 15:223-34. 2009
    ..The localizations of these enzymes were compared to known localizations of retinoid-binding proteins and associated proteins...
  90. ncbi NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranes
    Edward J Weinman
    Department of Medicine, Division Nephrology, University of Maryland School of Medicine, Room N3W143, UHM, 22 South Greene Street, Baltimore, MD 21201, USA
    FEBS Lett 536:141-4. 2003
    Sodium-hydrogen exchanger regulatory factor isoform-1 (NHERF-1) and NHERF-2 are two structurally related PDZ-domain-containing protein adapters that effectively transduce cyclic AMP (cAMP) signals that inhibit NHE3, the sodium-hydrogen ..
  91. pmc Microarray analysis of mercury-induced changes in gene expression in human liver carcinoma (HepG2) cells: importance in immune responses
    Wellington K Ayensu
    Cellomics and Toxicogenomics Research Laboratory, NIH RCMI Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, Jackson, Mississippi 39217, USA Email
    Int J Environ Res Public Health 3:141-73. 2006
    ..family protein, CDC-like kinase 1 (CLK1 HGNC) (protein tyrosine kinase STY), Na+/H+ exchanger regulatory factor (NHERF HGNC), potassium voltage-gated channel subfamily H member 2 (KCNH2), putative MAPK activating protein (PM20, PM21),..
  92. ncbi Signal complex regulation of renal transport proteins: NHERF and regulation of NHE3 by PKA
    E J Weinman
    Departments of Medicine and Physiology, University of Maryland School of Medicine, and Medical Service, Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201, USA
    Am J Physiol Renal Physiol 279:F393-9. 2000
    ..the essential requirement for a new class of regulatory factors, sodium/hydrogen exchanger regulatory factor (NHERF) and NHERF-like proteins, in cAMP-mediated inhibition of NHE3 activity...
  93. ncbi Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells
    Eleanor DeLand Lederer
    Louisville Veterans Administration Medical Center, Louisville, Kentucky, USA
    J Am Soc Nephrol 14:1711-9. 2003
    ..exchanger 3 by protein kinase A requires a protein co-factor from the sodium-hydrogen exchanger regulatory factor (NHERF) family of proteins (NHERF-1 and NHERF-2)...
  94. doi Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice
    Rochelle Cunningham
    Univ of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
    Am J Physiol Renal Physiol 294:F1001-7. 2008
    The adaptor proteins sodium/hydrogen exchanger regulatory factor (NHERF)-1 and NHERF-2 have overlapping tissue distribution in renal cells and overlapping specificity in their binding to renal transporters and other proteins...
  95. pmc Sodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 285:13454-60. 2010
    ..renal brush border membrane vesicles and in cultured renal proximal tubule cells from wild-type but not from NHERF-1 null mice. Co-immunoprecipitation experiments established that NHERF-1 associated with D1-like receptors...
  96. ncbi Sodium-phosphate cotransporters, nephrolithiasis and bone demineralization
    Dominique Prie
    Inserm U 426 et Institut Fédératif de Recherche 02, Faculte de Medecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
    Curr Opin Nephrol Hypertens 13:675-81. 2004
    ..We discuss how recent findings obtained in disorders of phosphate metabolism in humans and in animal models have provided insights into the pathogenesis of renal stone formation and bone demineralization...
  97. pmc The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a
    Edward J Weinman
    Department of Medicine, Division of Nephrology, University of Maryland, School of Medicine, 22, South Greene Street, Baltimore, MD 21202, USA
    J Physiol 567:27-32. 2005
    ..In the renal proximal tubule, three PDZ adaptor proteins, namely NHERF-1, NHERF-2 and PDZK1, are expressed in the apical membrane, heterodimerize with one another, and, at least in ..
  98. ncbi The association of NHERF adaptor proteins with g protein-coupled receptors and receptor tyrosine kinases
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, and Medical Service, Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201, USA
    Annu Rev Physiol 68:491-505. 2006
    The sodium-hydrogen exchanger regulatory factors (NHERF-1 and NHERF-2) are a family of adaptor proteins characterized by the presence of two tandem PDZ protein interaction domains and a C-terminal domain that binds the cytoskeleton ..
  99. pmc The enteropathogenic Escherichia coli effector protein EspF decreases sodium hydrogen exchanger 3 activity
    Kim Hodges
    Section of Digestive Disease and Nutrition, Department of Medicine, University of Illinois at Chicago, Jesse Brown VA Medical Center, Chicago, IL 60612, USA
    Cell Microbiol 10:1735-45. 2008
    ..The effect on NHE3 was enhanced when PS120 cells were co-transfected with the scaffolding/regulatory proteins NHERF1 or NHERF2 or EBP50 and E3KARP respectively...
  100. doi Parathyroid hormone signaling in bone and kidney
    Minnkyong Lee
    Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Curr Opin Nephrol Hypertens 18:298-302. 2009
    ..Here, we will review the recent advancements regarding PTH signaling in bone and kidney...
  101. ncbi NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats
    Ivan Sabolic
    Unit of Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
    Kidney Int 61:1351-64. 2002
    ..acutely regulated by a variety of mechanisms, including protein trafficking and interaction with the PDZ protein, NHERF. The effects of microtubule disruption by colchicine on NHE3 trafficking in PT and the potential role of NHERF in ..

Research Grants62

  1. NHERF-1 and dopamine-mediated regulation of renal phosphate transport.
    Edward J Weinman; Fiscal Year: 2013
    ..adaptor protein isolated and cloned by our laboratory called the Sodium-Hydrogen Exchanger Regulatory Factor-1 (NHERF-1)...
  2. Pradeep K Dudeja; Fiscal Year: 2016
    ..Aim 1 has been designed to yield critical mechanistic information on contribution of lipid rafts, cytoskeletal and NHERF proteins in EPEC and EspF- induced inhibition of NHE3 activity...
  3. Analysis of PTH and Dopamine Receptor Signaling in Proximal Tubules
    Eleanor D Lederer; Fiscal Year: 2013
    ..by applicant): Project Summary/Abstract The goal of this project is to determine how the structural protein NHERF-1, sodium-hydrogen exchanger regulatory factor isoform 1, regulates the trafficking of Npt2a, the type IIa sodium ..
  4. NHERF-1 and PTH regulation of the renal transport of phosphate.
    Edward J Weinman; Fiscal Year: 2012
    The Sodium-Hydrogen Exchanger Regulatory Factor-1 (NHERF-1) is an adaptor protein containing two protein-interactive PDZ domains and a C-terminal ERM binding domain that localizes to the brush border membrane of renal proximal ..
  5. PTH1R SIGNALING BY CYTOPLASMIC BINDING PARTNERS
    WILLIAM SNEDDON; Fiscal Year: 2005
    ..and how it is regulated by cytoplasmic adaptor proteins such as the sodium/hydrogen exchanger regulatory factor 1 (NHERF1), also known as the ezrin binding protein of 50 kDa (EBP50)...
  6. NHERFs Specify PTH Receptor Signaling
    Harald Juppner; Fiscal Year: 2005
    ..PLCbeta through PDZ domain-specific interactions; PLbeta beta 1 and 2 bind to PDZ1 and PTH1R binds to PDZ2 of both NHERF1 and 2...
  7. Hassane Amlal; Fiscal Year: 2014
    ..pathways mediating this effect will be investigated using both in vitro and in vivo studies involving PDZK1 and NHERF1 knout mice...
  8. Peter A Friedman; Fiscal Year: 2014
    ..The long-term goal of this project is to elucidate the cellular mechanisms by which the 50-kDa ezrin-bind protein (EBP50) regulates parathyroid hormone receptor (PTHR)-mediated signaling and function in bone...
  9. Salman Azhar; Fiscal Year: 2014
    ..Specifically, we propose to determine the stimulatory and inhibitory actions of SF-1/LRH-1 and NHERF1/2 and SIK-1 on SR-BI expression, SR-BI mediated selective CE transport and bile acid and steroid synthesis in ..
  10. Christopher S Wilcox; Fiscal Year: 2014
    ..We will study these effects on the NHE inhibitory protein-2 (NHERF-2) in vivo in the microperfused proximal tubules of WKY and SHR, by the addition of drugs to the perfusates, or ..
  11. Anthony P Bretscher; Fiscal Year: 2016
    ..The membrane-cytoskeletal linker ezrin, the ezrin-binding scaffolding protein EBP50 and its ligand EPI64 are key proteins that control microvilli on the apical aspect of cells, and this proposal is ..
  12. Transporters and Trafficking in Polarized Epithelial Cells
    Mark Donowitz; Fiscal Year: 2013
    ..BB PDZ protein knockout mice and knockdowns of individual NHERF proteins will be studied in Caco-2 cells to examine the role of these PDZ proteins in NHE3 regulation...
  13. Effects of NHERF1 expression on PTH1R dynamics and function in polarized cells
    DAVID SHERWOOD WHEELER; Fiscal Year: 2012
    ..example is the regulation of type 1 parathyroid hormone receptor (PTH1R) by NaVhT Exchange Regulatory Factor 1 (NHERF1)...
  14. Water and solute transport in the epididymis
    Sylvie Breton; Fiscal Year: 2010
    ..Interactions with CFTR, NHERF1 or PDZK1 will be studied...
  15. Small-Molecule Targeting of CFTR:PDZ Trafficking Interactions
    Dean R Madden; Fiscal Year: 2011
    ..Confirmed hits will be counterscreened for lack of inhibition of the NHERF1 and NHERF2 PDZ domains, whose interaction with CFTR stabilizes cell-surface expression of functional chloride ..
  16. Mark Donowitz; Fiscal Year: 2016
    ..Proteins shown to be part of this complex include NHERF 1-4, CaMKII, CK2, PLC?, and probably CaM and the complex is dynamic, changing in composition with conditions which ..
  17. Cytoskeletal linking proteins in renal epithelial cell function
    R Brian Doctor; Fiscal Year: 2012
    ..NaPiIIa activity is influence by its direct interaction with a number of distinct PDZ proteins, including EBP50 and PDZK1. These proteins appear to influence the delivery and retention of NaPiIIa in the apical membrane...
  18. Parathyroid Hormone Receptor Type 1: Dynamics, Traffic and Recycling
    Guillermo G Romero; Fiscal Year: 2010
    ..Abundant preliminary work presented here shows that the traffic the PTH1R is regulated by the PDZ proteins NHERF1 and Dvl2...
  19. G PROTEIN RECEPTOR - STRUCTURAL CHARACTERIZATION
    Dale F Mierke; Fiscal Year: 2010
    ..high-resolution NMR methods we will determine the structural features of the bimolecular interaction of NHERF (NHERF1 and NHERF2) with the C-termini of PTH1R and phospholipase C(beta) and use these results to rationally design ..
  20. PDZ scaffold regulation of astrocytic glutamate receptors and transporters
    Randy A Hall; Fiscal Year: 2012
    ..EAAT1 all exhibit robust and specific interactions with the PDZ domains of the multifunctional scaffold protein NHERF-2...
  21. Merry Jo Oursler; Fiscal Year: 2014
    ..hypothesis that early progenitor membrane expression levels of a PODXL membrane complex including CXCR4 and NHERF1 must be reduced to allow for precursor fusion and lower levels of this complex are required for activation of ..
  22. Keith E Mostov; Fiscal Year: 2015
    ..charged, transmembrane sialomucin, which binds via a PDZ motif at its C-terminus to the scaffolding protein NHERF1. We will mislocalize gp135 to the basolateral surface and see if NHERF1 and other proteins follow...
  23. Allan W Wolkoff; Fiscal Year: 2016
    ..In further studies, we showed that PDZK1 can bind to EBP50 (NHERF-1), a protein with two independent PDZ consensus binding sites...
  24. Astrocytic Scaffold Regulation of the Metabotropic Glutamate Receptor 3
    STEFANIE LEIGH RITTER; Fiscal Year: 2012
    ..found a novel interacting partner for mGluR3, known as the scaffold protein Na+/H+ exchanger regulatory factor 2 (NHERF-2). Interestingly, NHERF-2 is highly expressed in astrocytes, although little is known about its function...
  25. Jennifer D Black; Fiscal Year: 2016
    ..of ezrin at T567 locks ezrin into an open conformation thereby permitting its binding to a protein called EBP50 which is critical for the recruitment of ERM proteins that enable AKT signaling at the membrane...
  26. Chronic hypoxia and pH homeostasis in pulmonary myocytes
    Larissa A Shimoda; Fiscal Year: 2013
    ..also binds ezrin/radixin/ moesin-binding phosphoprotein of 50 kDa (EBP50), or Na+/H+ exchange regulatory factor 1 (NHERF1). NHERF1 acts as a scaffold linking together intracellular signaling complexes...
  27. Alexandra C Newton; Fiscal Year: 2014
    ..use innovative live cell imaging technologies to test the hypothesis that spatial coordination on the PDZ scaffold NHERF increases the ability of PHLPP to control the amplitude and duration of Akt signaling...
  28. A role for EPI64 in microvilli organization
    DAVID LALONDE; Fiscal Year: 2009
    ..In aim 2, the direct association between EPI64 and Arf6, as well as the EPI64 binding partners EBP50 and ezrin (which binds through EBP50) will be examined using purified proteins to determine if their direct binary ..
  29. MOLECULAR PATHOGENESIS OF HYPOPHOSPHATEMIC RICKETS
    Suzanne M Jan De Beur; Fiscal Year: 2011
    ..involving NaPi-lla and signaling molecules on the scaffold of Sodium hydrogen ion exchanger related factor-1 (NHERF-1) that interacts with the actin cytoskeleton...
  30. Regulation of Renal Phosphate Transport by Lipids
    Moshe Levi; Fiscal Year: 2013
    ..in the brush border membrane;2) differential interactions with PDZ and ERM domain containing proteins, including NHERF-1, PDZK-1, and ezrin;3) differential rates of recruitment to endocytic pathways and/or differential mechanisms of ..
  31. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..follow changes in the expression and localization of cell-specific proteins and mRNA (V-ATPase subunit, AE1, AE4, NHERF, AQP2, AQP4, V2R) during cell remodeling...
  32. Heinrich Roder; Fiscal Year: 2014
    ..detailed studies of the structure, dynamics and binding properties of Na+/H+ exchanger regulatory factor 1 (NHERF1), a signaling adaptor comprised of two globular domains belonging to the large PDZ fold family, a C-terminal ezrin-..
  33. NHERF2 Directly Mediates PTH/PTHrP Receptor Signaling
    MATTHEW MAHON; Fiscal Year: 2004
    ..Phospholipase Cbeta1 and beta2 interact with NHERF proteins...
  34. SCOR: CFTR IN AIRWAY CELL FUNCTION
    RAYMOND FRIZZELL; Fiscal Year: 2004
    ..This project will examine CFTR's interaction with anchored regulators (including ezrin and EBP50) in an intracellular compartment that is enriched in CFTR and type II protein kinase A, the clathrin coted ..
  35. Molecular Mechanisms of CFTR Regulation
    JOHN LADIAS; Fiscal Year: 2006
    ..of the R domain, ATP hydrolysis by the NBDs, and interactions of its NTD and CTD domains with syntaxin 1A and NHERF proteins, respectively...
  36. Modulation of NHE Activity by EPEC Infection
    Pradeep Dudeja; Fiscal Year: 2006
    ..modulation of NHE isoform activities by elucidating signaling pathways involved, the role of regulatory factors NHERF1 and NHERF2), the role of the cytoskeletal protein ezrin, and NHE membrane trafficking; 3...
  37. Cytoskeletal Linking Proteins in Liver Function
    R Brian Doctor; Fiscal Year: 2006
    ..In epithelial cells, Ezrin-Radixin-Moesin binding phosphoprotein 50 (EBP50) has been implicated in modulating cAMP-dependent apical transport events...
  38. MECHANISMS OF BILE SECRETION AND CHOLESTASIS
    James Boyer; Fiscal Year: 2007
    ..The role of "tethering" proteins (ezrin, radixin, EKARP, EBP50) in the targeting of Mrp2, Bsep and Mdr1 to the canalicular domain in hepatocytes will also be examined; (3) ..
  39. Role and Regulation of Intestinal Na/H Exchanger
    Eugene Chang; Fiscal Year: 2007
    ..anion exchanger, DRA (Down-Regulated in Adenoma), the dipeptidy1 transporter hPepT1, adaptor proteins (E3KARP and NHERF), and other key molecules such as v- and t-SNARES required for membrane endo- and exocytosis...
  40. ENDOTHELIAL CELL CGMP RECEPTORS AND FUNCTION
    W Thompson; Fiscal Year: 2000
    ..The phosphoprotein NHE regulatory factor (NHERF) is proposed to mediate cAMP regulation of the NHE...
  41. NHERF IN BETA-2-ADRENERGIC RECEPTOR SIGNALING
    Randy Hall; Fiscal Year: 2003
    ..coupling of the beta2AR to an intracellular protein called the Na+/H+ exchanger regulatory factor (NHERF). The mechanisms and potential generality of this NHERF-mediated signaling by the beta2AR are unknown...
  42. E3KARP and cGMP regulation of NHE3
    Mark Donowitz; Fiscal Year: 2003
    ..in which cAMP did not have any effect, cAMP inhibition of NHE3 could be reconstituted when the regulatory factors, NHERF or E3KARP, were present...
  43. PTH Receptors in Vascular Smooth Muscle Cells
    Alessandro Bisello; Fiscal Year: 2009
    ..Recent evidence shows that ezrin-binding protein 50 KDa (EBP50) contributes to signaling specificity and trafficking of the PTH1R...
  44. SCAFFOLDING COMPLEXES AND ION TRANSPORT IN AIRWAY
    Sharon Milgram; Fiscal Year: 2004
    ..at the apical membrane by indirect interaction with the subapical scaffolding protein ERM phosphoprotein 50 (EBP50) that also associates with Cystic Fibrosis transmembrane regulator (CFTR) and ENaC...
  45. Professional Development Seminar: Early and Mid-Career Components
    Rochelle Cunningham; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  46. Phosphatase Inhibitor-1, An Amplifier of cAMP Signals
    Shirish Shenolikar; Fiscal Year: 2005
    ..2) Define the role of GADD34 in the assembly of the mammalian eIF2alpha phosphatase and the transduction of physiological signals that regulate protein synthesis and dictate the switch between cell death and survival. ..
  47. Protein Phosphatase-1-Targeting the Cytoskeleton
    Shirish Shenolikar; Fiscal Year: 2005
    ..Elucidating neurabin-mediated PP1 signaling may define the defects in plasticity that contribute to mental retardation, epilepsy and neurodegenerative disease. ..
  48. Regulation of CFTR by Protein Kinase C
    CAROLE LIEDTKE; Fiscal Year: 2004
    ..Sequence motifs in specific domains of PKC-epsilon and/or binding will be predicted and tested using peptides to inhibit binding and cAMP-dependent activation of CFTR. ..
  49. Connecting PTEN and NF2 cancer predisposition syndromes
    Maria Magdalena Georgescu; Fiscal Year: 2009
    ..abstract_text> ..
  50. Gordon Research Conference on Calcium Signaling
    Colin Taylor; Fiscal Year: 2005
    ..abstract_text> ..
  51. GENETIC ANALYSIS OF PSORIASIS
    Anne Bowcock; Fiscal Year: 2001
    ..When alterations are found, they will be screened for in 100 unrelated controls to determine the possibility of their contributing to the development of psoriasis. ..
  52. REGULATION OF KAPPA OPIOID RECEPTORS
    Lee Yuan Liu Chen; Fiscal Year: 2002
    ..Elucidation of the mechanisms involved in agonist-induced regulation of the KOR will lead to a better understanding of tolerance to kappa agonist, which will help in the development of better kappa agonists as useful drugs. ..
  53. TEM with Digital and Cryopreparation Systems
    Andrew Evan; Fiscal Year: 2003
    ..Dr. Richard Gregory will employ immunohistochemistry to examine the role of a Streptococcus mutans 65 kDa fimbrial protein binding as a mechanism for the induction of dental carries. ..
  54. Sequenom MassARTRAY System
    Anne Bowcock; Fiscal Year: 2003
    ..At the end of the grant period it will be incorporated into a core genotyping facility within the Division of Human Genetics. ..
  55. MOLECULAR CHARACTERIZATION OF OPIOID RECEPTORS
    Lee Yuan Liu Chen; Fiscal Year: 2004
    ..The information will provide insights into the structures of the binding-site crevices of opioid receptors, which will aid in the design of selective agonists and antagonists for prevention and treatment of drug abuse. ..
  56. SYK SIGNALING AND BREAST CANCER INVASION
    JIALE DAI; Fiscal Year: 2007
    ..2). Taken together, these studies should clarify the mechanisms by which the loss of Syk(L) and aberrant expression of Syk(S) promotes breast cancer progression. [unreadable] [unreadable]..
  57. IKSI: 1st Annual International Urolithiasis Research Symposium
    Andrew Evan; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  58. Vibrio Cholerae as a bioterror agent: New means of treating a potential pandemic
    Anjaparavanda Naren; Fiscal Year: 2008
    ..cholerae or due to natural causes. [unreadable] [unreadable] [unreadable]..
  59. DERMATOLOGY TRAINING GRANT
    Anne Bowcock; Fiscal Year: 2008
    ..We are currently recruiting two tenure-track faculty members and are confident that this will only serve to further strengthen this program, and the quality of scientific opportunities afforded to the trainees. ..
  60. NOVEL REGULATORY MECHANISMS CONTROLLING BONE REPAIR AND OSTEOPOROSIS
    Peter Friedman; Fiscal Year: 2008
    ..uncoupling of PTH1R activation and inactivation is due to the presence of a cytoplasmic adaptor protein, termed EBP50 (ezrin-binding protein, 50 kDa). EBP50 contains an estrogen response element...
  61. IKSI: 2nd International Urolithiasis Research Symposium
    Andrew Evan; Fiscal Year: 2008
    ..This assumption was supported by the great success of the 1st International Urolithiasis Research symposium and the high level of interest in the 2nd symposium date. [unreadable] [unreadable] [unreadable]..
  62. Bladder-specific Knockout and Gene Expression
    Xue ru Wu; Fiscal Year: 2009
    ..These studies will generate powerful experimental tools for studying bladder biology and diseases and offer molecular insights regarding urothelial growth and tumorigenesis. ..