SLC46A1

Summary

Gene Symbol: SLC46A1
Description: solute carrier family 46 member 1
Alias: G21, HCP1, PCFT, proton-coupled folate transporter, heme carrier protein 1, solute carrier family 46 (folate transporter), member 1
Species: human

Top Publications

  1. ncbi Identification of an intestinal heme transporter
    Majid Shayeghi
    Department of Life Sciences, Nutritional Sciences Research Division, Franklin Wilkins Building, Kings College London, 150 Stamford Street, London SE1 9NN, UK
    Cell 122:789-801. 2005
  2. pmc A role for the proton-coupled folate transporter (PCFT-SLC46A1) in folate receptor-mediated endocytosis
    Rongbao Zhao
    Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 284:4267-74. 2009
  3. ncbi Heme carrier protein (HCP-1) spatially interacts with the CD163 hemoglobin uptake pathway and is a target of inflammatory macrophage activation
    Christian A Schaer
    Department of Medicine, University Hospital, CH 8091 Zurich, Switzerland
    J Leukoc Biol 83:325-33. 2008
  4. doi Heme carrier protein 1 transports heme and is involved in heme-Fe metabolism
    Solange Le Blanc
    Micronutrient Laboratory, Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile
    Am J Physiol Cell Physiol 302:C1780-5. 2012
  5. pmc The clinical course and genetic defect in the PCFT gene in a 27-year-old woman with hereditary folate malabsorption
    Sang Hee Min
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA
    J Pediatr 153:435-7. 2008
  6. doi PCFT/SLC46A1 promoter methylation and restoration of gene expression in human leukemia cells
    Nitzan Gonen
    The Fred Wyszkowski Cancer Research Laboratory, Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Biochem Biophys Res Commun 376:787-92. 2008
  7. doi Functional elements in the minimal promoter of the human proton-coupled folate transporter
    Michal Stark
    Dept of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Biochem Biophys Res Commun 388:79-85. 2009
  8. pmc Identification of a functionally critical GXXG motif and its relationship to the folate binding site of the proton-coupled folate transporter (PCFT-SLC46A1)
    Rongbao Zhao
    Depts of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Am J Physiol Cell Physiol 303:C673-81. 2012
  9. ncbi Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption
    Andong Qiu
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell 127:917-28. 2006
  10. ncbi Haem carrier protein 1 (HCP1): Expression and functional studies in cultured cells
    Gladys O Latunde-Dada
    Department of Biochemistry, School of Biomedical and Health Sciences, Franklin Wilkins Building, King s College London, 150 Stamford Street, London, SE1 9NH, United Kingdom
    FEBS Lett 580:6865-70. 2006

Research Grants

  1. ISRAEL DAVID GOLDMAN; Fiscal Year: 2016
  2. Larry H Matherly; Fiscal Year: 2015
  3. Larry H Matherly; Fiscal Year: 2016
  4. Novel Type IV Pili of Enterohemorrhagic E. coli O157:H7
    Jorge Giron; Fiscal Year: 2004
  5. Ferritin and Iron Nutrition in Health and Disease
    Elizabeth Theil; Fiscal Year: 2005
  6. Quantitation of In Vivo Human Lutein Metabolism
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010
  7. DYNAMIC AND KINETIC BEHAVIOR OF FOLATE METABOLISM
    Andrew Clifford; Fiscal Year: 2005
  8. IN VIVO DYNAMIC OF BETA-CAROTENE METABOLISM IN HUMANS
    Andrew Clifford; Fiscal Year: 2006
  9. Intestinal Iron Transport in Iron Deficiency/Anemia
    James Collins; Fiscal Year: 2005
  10. Quantitation of Tocopherol Metabolism in Humans
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications159 found, 100 shown here

  1. ncbi Identification of an intestinal heme transporter
    Majid Shayeghi
    Department of Life Sciences, Nutritional Sciences Research Division, Franklin Wilkins Building, Kings College London, 150 Stamford Street, London SE1 9NN, UK
    Cell 122:789-801. 2005
    ..Here we show a membrane protein named HCP 1 (heme carrier protein 1), with homology to bacterial metal-tetracycline transporters, mediates heme uptake by cells in a ..
  2. pmc A role for the proton-coupled folate transporter (PCFT-SLC46A1) in folate receptor-mediated endocytosis
    Rongbao Zhao
    Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 284:4267-74. 2009
    Recently, this laboratory identified a proton-coupled folate transporter (PCFT), with optimal activity at low pH...
  3. ncbi Heme carrier protein (HCP-1) spatially interacts with the CD163 hemoglobin uptake pathway and is a target of inflammatory macrophage activation
    Christian A Schaer
    Department of Medicine, University Hospital, CH 8091 Zurich, Switzerland
    J Leukoc Biol 83:325-33. 2008
    ..In this study, we show that the heme transporter heme carrier protein 1 (HCP-1) is expressed in human macrophages...
  4. doi Heme carrier protein 1 transports heme and is involved in heme-Fe metabolism
    Solange Le Blanc
    Micronutrient Laboratory, Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile
    Am J Physiol Cell Physiol 302:C1780-5. 2012
    ..b>Heme carrier protein 1 (HCP1) was originally identified as mediating heme-Fe transport although it later emerged that it was a ..
  5. pmc The clinical course and genetic defect in the PCFT gene in a 27-year-old woman with hereditary folate malabsorption
    Sang Hee Min
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA
    J Pediatr 153:435-7. 2008
    We report 2 sequential homozygous mutations in the recently cloned proton-coupled folate transporter (PCFT) gene, resulting in the absence of this protein, in a 27-year-old woman with hereditary folate malabsorption, normal in all ..
  6. doi PCFT/SLC46A1 promoter methylation and restoration of gene expression in human leukemia cells
    Nitzan Gonen
    The Fred Wyszkowski Cancer Research Laboratory, Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Biochem Biophys Res Commun 376:787-92. 2008
    The proton-coupled folate transporter (PCFT/SLC46A1) displays optimal and prominent folate and antifolate transport activity at acidic pH in human carcinoma cells but poor activity in leukemia cells...
  7. doi Functional elements in the minimal promoter of the human proton-coupled folate transporter
    Michal Stark
    Dept of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Biochem Biophys Res Commun 388:79-85. 2009
    The proton-coupled folate transporter (PCFT) is the dominant intestinal folate transporter, however, its promoter has yet to be revealed. Hence, we here cloned a 3...
  8. pmc Identification of a functionally critical GXXG motif and its relationship to the folate binding site of the proton-coupled folate transporter (PCFT-SLC46A1)
    Rongbao Zhao
    Depts of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Am J Physiol Cell Physiol 303:C673-81. 2012
    The proton-coupled folate transporter (PCFT) mediates intestinal folate absorption, and loss-of-function mutations in this gene result in the autosomal recessive disorder hereditary folate malabsorption...
  9. ncbi Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption
    Andong Qiu
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell 127:917-28. 2006
    ..However, the current study establishes that a major function of this gene product is proton-coupled folate transport required for folate homeostasis in man, and we have thus amended the name to PCFT/HCP1.
  10. ncbi Haem carrier protein 1 (HCP1): Expression and functional studies in cultured cells
    Gladys O Latunde-Dada
    Department of Biochemistry, School of Biomedical and Health Sciences, Franklin Wilkins Building, King s College London, 150 Stamford Street, London, SE1 9NH, United Kingdom
    FEBS Lett 580:6865-70. 2006
    ..The current studies describe the expression and functional analysis of HCP1 in cultured Caco-2 cells, a commonly used model of human intestinal cells...
  11. pmc The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary folate malabsorption
    Rongbao Zhao
    Departments of Medicine, Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Blood 110:1147-52. 2007
    ..studies in 1 family revealed that the molecular basis for this disorder is a loss-of-function mutation in the PCFT gene encoding a proton-coupled folate transporter...
  12. ncbi Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter
    Yasuhiro Nakai
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya 467 8603, Japan
    J Pharmacol Exp Ther 322:469-76. 2007
    The functional characteristics of human proton coupled folate transporter (hPCFT)/heme carrier protein (HCP) 1 were investigated...
  13. pmc Synthesis and biological activity of a novel series of 6-substituted thieno[2,3-d]pyrimidine antifolate inhibitors of purine biosynthesis with selectivity for high affinity folate receptors over the reduced folate carrier and proton-coupled folate transpo
    Yijun Deng
    Graduate Program in Cancer Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Med Chem 52:2940-51. 2009
    ..ovary (CHO) cells that expressed FRs but not the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT). However, the compounds were inactive toward CHO cells that lacked FRs but contained either the RFC or PCFT...
  14. pmc Role of the glutamate 185 residue in proton translocation mediated by the proton-coupled folate transporter SLC46A1
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 297:C66-74. 2009
    The proton-coupled folate transporter (PCFT) SLC46A1 mediates uphill folate transport into enterocytes in proximal small intestine coupled to the inwardly directed proton gradient...
  15. doi The human proton-coupled folate transporter (hPCFT): modulation of intestinal expression and function by drugs
    Bradley L Urquhart
    Division of Clinical Pharmacology, Department of Medicine, University of Western Ontario, London Health Sciences Centre Univ Campus, 339 Windermere Rd, London, Ontario, Canada
    Am J Physiol Gastrointest Liver Physiol 298:G248-54. 2010
    ..Importantly, we note that PPI drug use appears to be associated with reduced hPCFT expression in vivo...
  16. pmc A novel PCFT gene mutation (p.Cys66LeufsX99) causing hereditary folate malabsorption
    Esther Meyer
    Department of Medical and Molecular Genetics, Institute of Biomedical Research, University of Birmingham, Birmingham B15 2TT, UK
    Mol Genet Metab 99:325-8. 2010
    ..Mutations in the intestinal folate transporter PCFT have been reported previously in only 10 individuals with this disorder...
  17. pmc Membrane topological analysis of the proton-coupled folate transporter (PCFT-SLC46A1) by the substituted cysteine accessibility method
    Rongbao Zhao
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 49:2925-31. 2010
    The proton-coupled folate transporter (PCFT) mediates intestinal folate absorption. Loss-of-function mutations in this gene are the molecular basis for the autosomal recessive disorder, hereditary folate malabsorption...
  18. pmc Properties of the Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary folate malabsorption
    Kris Mahadeo
    Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 299:C1153-61. 2010
    The proton-coupled folate transporter (PCFT-SLC46A1) is required for intestinal folate absorption and is mutated in the autosomal recessive disorder, hereditary folate malabsorption (HFM)...
  19. pmc The obligatory intestinal folate transporter PCFT (SLC46A1) is regulated by nuclear respiratory factor 1
    Nitzan Gonen
    Fred Wyszkowski Cancer Research Laboratory, Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 285:33602-13. 2010
    ..The proton-coupled folate transporter (PCFT/SLC46A1) mediates obligatory intestinal folate absorption...
  20. pmc Mutation of the proton-coupled folate transporter gene (PCFT-SLC46A1) in Turkish siblings with hereditary folate malabsorption
    Berna Atabay
    Clinics of Pediatrics, Tepecik Training and Research Hospital, Izmir, Turkey
    Pediatr Hematol Oncol 27:614-9. 2010
    ..204_205 delCC) within the first exon of the proton-coupled folate transporter (PCFT) gene, causing a frameshift. Low blood and cerebrospinal fluid folate levels were detected at ages 3.5 and 1 month...
  21. pmc Functional roles of aspartate residues of the proton-coupled folate transporter (PCFT-SLC46A1); a D156Y mutation causing hereditary folate malabsorption
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA
    Blood 116:5162-9. 2010
    The proton-coupled folate transporter (PCFT; SLC46A1) mediates folate transport into enterocytes in the proximal small intestine; pcft loss-of-function mutations are the basis for hereditary folate malabsorption...
  22. pmc Identification of novel mutations in the proton-coupled folate transporter (PCFT-SLC46A1) associated with hereditary folate malabsorption
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Genet Metab 103:33-7. 2011
    ..disorder, recently shown to be due to loss-of-function mutations of the proton-coupled folate transporter (PCFT-SLC46A1), resulting in systemic and central nervous system folate deficiency...
  23. pmc Random mutagenesis of the proton-coupled folate transporter (SLC46A1), clustering of mutations, and the bases for associated losses of function
    Rongbao Zhao
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 286:24150-8. 2011
    Loss-of-function mutations in the proton-coupled folate transporter (PCFT, SLC46A1) result in the autosomal recessive disorder, hereditary folate malabsorption (HFM)...
  24. pmc Identification and functional impact of homo-oligomers of the human proton-coupled folate transporter
    Zhanjun Hou
    Developmental Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 287:4982-95. 2012
    The proton-coupled folate transporter (PCFT; SLC46A1) is a proton-folate symporter that is abundantly expressed in solid tumors and normal tissues, such as duodenum...
  25. pmc A P425R mutation of the proton-coupled folate transporter causing hereditary folate malabsorption produces a highly selective alteration in folate binding
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 302:C1405-12. 2012
    Proton-coupled folate transporter (PCFT) mediates folate intestinal absorption and transport across the choroid plexus, processes defective in subjects with hereditary folate malabsorption (HFM)...
  26. ncbi Apical membrane targeting and trafficking of the human proton-coupled transporter in polarized epithelia
    Veedamali S Subramanian
    Departments of Medicine, University of California, Irvine, California, USA
    Am J Physiol Cell Physiol 294:C233-40. 2008
    ..For the first time, our data present information regarding structure-function and membrane targeting of the hPCFT polypeptide, as well as the mechanisms that control its steady-state expression in polarized cells...
  27. pmc The functional roles of the His247 and His281 residues in folate and proton translocation mediated by the human proton-coupled folate transporter SLC46A1
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Biol Chem 284:17846-57. 2009
    This report addresses the functional role of His residues in the proton-coupled folate transporter (PCFT; SLC46A1), which mediates intestinal folate absorption. Of ten His residues, only H247A and H281A mutations altered function...
  28. doi A genomic island of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough promotes survival under stress conditions while decreasing the efficiency of anaerobic growth
    Shawna Johnston
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada
    Environ Microbiol 11:981-91. 2009
    ..recombinases and transposases, rubredoxin:oxygen oxidoreductase-1 (Roo1) and hybrid cluster protein-1 (Hcp1), which promote survival in air and nitrite stress...
  29. ncbi Quantitation of toxic arsenic species and arsenobetaine in Pacific oysters using an off-line process with hydride generation-atomic absorption spectroscopy
    Tung Ming Hsiung
    Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, Republic of China
    J Agric Food Chem 54:2470-8. 2006
    ..were determined with a sensitive hydride generation-packed coldfinger trap-atomic absorption spectrometric (HG-PCFT-AAS) coupled system...
  30. doi Characterization of uptake of folates by rat and human blood-brain barrier endothelial cells
    Joao R Araujo
    Department of Biochemistry U38 FCT, Faculty of Medicine, University of Porto, Porto, Portugal
    Biofactors 36:201-9. 2010
    ..expression of reduced folate transporter (RFC), but neither of FRalpha nor of proton-coupled folate transporter (PCFT) in RBE4 cells, and mRNA expression of RFC and PCFT, but not of FRalpha, in hCMEC/D3 cells...
  31. pmc H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Exp Physiol 92:603-19. 2007
    ..OATP2B1 (SLC02B1); the monocarboxylate transporter MCT1 (SLC16A1); the proton-coupled folate transporter PCFT (SLC46A1); the sodium-glucose linked cotransporter SGLT1 (SLC5A1); and the excitatory amino acid carrier EAAC1 (SLC1A1)...
  32. ncbi Characterization of a novel barley protein, HCP1, that interacts with the Brome mosaic virus coat protein
    Yasushi Okinaka
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Mol Plant Microbe Interact 16:352-9. 2003
    ..One of the candidate cDNAs encodes a unique oxidoreductase enzyme, designated HCP1. HCP1 was found predominantly in the soluble fractions after differential centrifugation of BMV-infected and mock-..
  33. doi Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model
    Katsuhisa Inoue
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho ku, Nagoya 467 8603, Japan
    Am J Physiol Gastrointest Liver Physiol 294:G660-8. 2008
    Proton-coupled folate transporter/heme carrier protein 1 (PCFT/HCP1) has recently been identified as a transporter that mediates the translocation of folates across the cellular membrane by a proton-coupled mechanism and suggested to be ..
  34. pmc The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei
    Mary N Burtnick
    Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama 36688, USA
    Infect Immun 79:1512-25. 2011
    ..Recombinant Hcp2 protected 80% of mice against a lethal challenge with K96243, while recombinant Hcp1, Hcp3, and Hcp6 protected 50% of mice against challenge. Hcp6 was the only Hcp constitutively produced by B...
  35. ncbi Fifteen-year follow-up of 92 hospitalized adults with Down's syndrome: incidence of cognitive decline, its relationship to age and neuropathology
    M L Margallo-Lana
    Prudhoe Hospital, Northumberland, UK
    J Intellect Disabil Res 51:463-77. 2007
    ..The clinical and neuropathological features associated with dementia in Down's syndrome (DS) are not well established. Aims To examine clinico-pathological correlations and the incidence of cognitive decline in a cohort of adults with DS...
  36. doi Effect of cannabinoids upon the uptake of folic acid by BeWo cells
    Joao R Araujo
    Department of Biochemistry U38 FCT, Faculty of Medicine, University of Porto, Porto, Portugal
    Pharmacology 83:170-6. 2009
    ..The acute effects of cannabinoid receptor agonists do not seem to be mediated by the cannabinoid receptor, and with a few exceptions the chronic effects do not seem to be related to changes in the expression levels of FA transporters...
  37. ncbi Involvement of reduced folate carrier 1 in the inner blood-retinal barrier transport of methyltetrahydrofolate
    Ken ichi Hosoya
    Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Japan
    Drug Metab Pharmacokinet 23:285-92. 2008
    ..Although both RFC1 and proton-coupled folate transporter (PCFT) mRNA, which are pH-sensitive folate transporters, are expressed in TR-iBRB2 cells and isolated rat retinal ..
  38. ncbi Rodent intestinal folate transporters (SLC46A1): secondary structure, functional properties, and response to dietary folate restriction
    Andong Qiu
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 293:C1669-78. 2007
    ..a human gene that encodes a novel folate transporter [Homo sapiens proton-coupled folate transporter (HsPCFT); SLC46A1] required for intestinal folate absorption...
  39. doi Proton-coupled folate transporter (PCFT): molecular cloning, tissue expression patterns and the effects of dietary folate supplementation on mRNA expression in laying hens
    M Jing
    Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada
    Br Poult Sci 51:635-8. 2010
    ..and effects of dietary folate supplementation on mRNA concentrations of the proton-coupled folate transporter (PCFT) in chicken. 2...
  40. ncbi Isolation and characterization of a previously undetected human cAMP phosphodiesterase by complementation of cAMP phosphodiesterase-deficient Saccharomyces cerevisiae
    T Michaeli
    Cold Spring Harbor Laboratory, New York 11724
    J Biol Chem 268:12925-32. 1993
    ..Two of these genes are closely related to the Drosophila dunce cAMP-specific PDE. The third gene, which we named HCP1, encoded a novel cAMP-specific PDE...
  41. pmc N-linked glycosylation and its impact on the electrophoretic mobility and function of the human proton-coupled folate transporter (HsPCFT)
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochim Biophys Acta 1778:1407-14. 2008
    The human proton-coupled folate transporter (HsPCFT, SLC46A1) mediates intestinal absorption of folates and transport of folates into the liver, brain and other tissues...
  42. doi Molecular and functional characteristics of proton-coupled folate transporter
    Hiroaki Yuasa
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya 467 8603, Japan
    J Pharm Sci 98:1608-16. 2009
    Proton-coupled folate transporter (PCFT) has recently been identified as the molecular entity of the carrier-mediated intestinal folate transport system...
  43. ncbi Primary carcinoma of the fallopian tube and epithelial ovarian carcinoma: a case-control analysis
    Melissa S Dunn
    Division of Gynecologic Oncology, Holden Comprehensive Cancer Center, University of Iowa, Iowa City, USA
    J Reprod Med 53:691-4. 2008
    To determine whether differences exist in clinicopathologic variables or survival between women with primary carcinoma of the fallopian tube (PCFT) and with epithelial ovarian carcinoma (EOC).
  44. pmc The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system
    Lisa G Pell
    Department of Biochemistry, University of Toronto, Medical Sciences Building, Toronto, ON M5S 1A8, Canada
    Proc Natl Acad Sci U S A 106:4160-5. 2009
    ..A remarkable structural similarity is also seen to Hcp1, a component of the bacterial type VI secretion system...
  45. ncbi Identification of proton-coupled high-affinity human intestinal folate transporter mutated in human hereditary familial folate malabsorption
    George Wolf
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California, USA
    Nutr Rev 65:554-7. 2007
    ..The transporter, named PCFT/HCP1, is a protein of approximately 50 kDa and functions as a reversible, electrogenic, proton-coupled high-..
  46. pmc In vitro self-assembly of tailorable nanotubes from a simple protein building block
    Edward R Ballister
    Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:3733-8. 2008
    ..for generating discretely structured protein nanotubes from the simple ring-shaped building block, homohexameric Hcp1 from Pseudomonas aeruginosa...
  47. doi Comparison of short and long versions of the Prudhoe Cognitive Function Test and the K-BIT in participants with intellectual impairment
    Stephen P Tyrer
    Department of Psychiatry, Newcastle University, Royal Victoria Infirmary, Leazes Wing, Newcastle upon Tyne, UK
    J Autism Dev Disord 40:1000-5. 2010
    The Prudhoe Cognitive Function Test (PCFT) directly measures the cognitive abilities of people with intellectual impairment...
  48. ncbi The Prudhoe Cognitive Function Test, a scale to assess cognitive function in adults with Down's syndrome: inter-rater and test-retest reliability
    M L Margallo-Lana
    Department of Psychiatry, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
    J Intellect Disabil Res 47:488-92. 2003
    The Prudhoe Cognitive Function Test (PCFT) was designed to measure cognitive function in subjects with all degrees of intellectual disability (ID) and to be administered by non-specialist raters...
  49. ncbi Novel insights into intestinal and renal folate transport. Focus on "Apical membrane targeting and trafficking of the human proton-coupled folate transporter in polarized epithelia"
    James F Collins
    Department of Exercise and Nutrition Sciences, University at Buffalo, The State University of New York, 3435 Main St, G10 Farber Hall, Buffalo, NY 14214 8001, USA
    Am J Physiol Cell Physiol 294:C381-2. 2008
  50. doi Expression of folate transporters in human placenta and implications for homocysteine metabolism
    N Solanky
    School of Clinical and Laboratory Sciences, University of Manchester, Manchester Academic Health Science Centre, St Mary s Hospital, Manchester, UK
    Placenta 31:134-43. 2010
    ..These data support the concept that placental folate transport is established early in pregnancy, providing folate for utilisation in placental Hcy metabolism...
  51. pmc Molecular and biochemical characterization of folate transport proteins in retinal Müller cells
    B Renee Bozard
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, Georgia 30912 2000, USA
    Invest Ophthalmol Vis Sci 51:3226-35. 2010
    ..To analyze the mechanisms of folate uptake in retinal Müller cells...
  52. pmc Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda
    Chacko Jobichen
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 5:e12910. 2010
    ..EvpC is one of the three major EVP secreted proteins and shares high sequence similarity with Hcp1, a key T6SS virulence factor from Pseudomonas aeruginosa. EvpC contributes to the virulence of E...
  53. doi Reduced levels of folate transporters (PCFT and RFC) in membrane lipid rafts result in colonic folate malabsorption in chronic alcoholism
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India
    J Cell Physiol 226:579-87. 2011
    ..transport activity at the CAM was associated with down-regulation of the proton-coupled folate transporter (PCFT) and the reduced folate carrier (RFC) which resulted in decreased PCFT and RFC protein levels in the colon of rats ..
  54. pmc Synthesis and biological activity of 6-substituted pyrrolo[2,3-d]pyrimidine thienoyl regioisomers as inhibitors of de novo purine biosynthesis with selectivity for cellular uptake by high affinity folate receptors and the proton-coupled folate transporter
    Lei Wang
    Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, Pennsylvania 15282, United States
    J Med Chem 55:1758-70. 2012
    ..3b was completely selective for transport by folate receptors (FRs) and the proton-coupled folate transporter (PCFT) over the reduced folate carrier (RFC), 3a was not...
  55. doi Rare allelic variants determine folate status in an unsupplemented European population
    Marketa Pavlikova
    Department of Medical Informatics, Institute of Computer Science of the Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Nutr 142:1403-9. 2012
    ..intronic regions of 6 candidate genes involved in folate metabolism or transport (FOLR1, FOLR2, FOLR3, MTHFR, PCFT, and RFC) from 29 individuals with low plasma and erythrocyte folate concentrations...
  56. pmc Therapeutic targeting malignant mesothelioma with a novel 6-substituted pyrrolo[2,3-d]pyrimidine thienoyl antifolate via its selective uptake by the proton-coupled folate transporter
    Christina Cherian
    Department of Oncology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cancer Chemother Pharmacol 71:999-1011. 2013
    ..thienoyl antifolate, compound 2, might be an effective treatment for malignant pleural mesothelioma (MPM), reflecting its selective membrane transport by the proton-coupled folate transport (PCFT) over the reduced folate carrier (RFC).
  57. pmc Folate and thiamine transporters mediated by facilitative carriers (SLC19A1-3 and SLC46A1) and folate receptors
    Rongbao Zhao
    Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Aspects Med 34:373-85. 2013
    ..The proton-coupled folate transporter (PCFT, SLC46A1) is the mechanism by which folates are absorbed across the apical-brush-border membrane of the proximal small ..
  58. pmc Inhibition of the proton-coupled folate transporter (PCFT-SLC46A1) by bicarbonate and other anions
    Rongbao Zhao
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Chanin 628, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Pharmacol 84:95-103. 2013
    The proton-coupled folate transporter (PCFT) plays a key role in intestinal folate absorption, and loss-of-function mutations in the gene encoding this transporter are the molecular basis for hereditary folate malabsorption...
  59. pmc Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila
    J Bernard Heymann
    NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:25276-84. 2013
    ..The central tube is similar to the diameter and axial width of the Hcp1 hexamer of P. aeruginosa type VI secretion system...
  60. doi Stereoselectivity of chiral drug transport: a focus on enantiomer-transporter interaction
    Quan Zhou
    Department of Pharmacy, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China and
    Drug Metab Rev 46:283-90. 2014
    ..Organic Cation Transporters (OCTs), Peptide Transport Proteins (PepTs), Human Proton-Coupled Folate Transporter (PCFT) and Multidrug and Toxic Extrusion Proteins (MATEs), have been reported to exhibit either positive or negative ..
  61. pmc Substituted cysteine accessibility reveals a novel transmembrane 2-3 reentrant loop and functional role for transmembrane domain 2 in the human proton-coupled folate transporter
    Mike R Wilson
    From the Department of Oncology and
    J Biol Chem 289:25287-95. 2014
    The proton-coupled folate transporter (PCFT) is a folate-proton symporter highly expressed in solid tumors that can selectively target cytotoxic antifolates to tumors under acidic microenvironment conditions...
  62. doi SLC19A1, SLC46A1 and SLCO1B1 polymorphisms as predictors of methotrexate-related toxicity in Portuguese rheumatoid arthritis patients
    Aurea Lima
    CESPU, Institute of Research and Advanced Training in Health Sciences and Technologies, Department of Pharmaceutical Sciences, Higher Institute of Health Sciences North ISCS N, Rua Central de Gandra 1317, 4585 116 Gandra PRD, Portugal Molecular Oncology Group CI, Portuguese Institute of Oncology of Porto IPO Porto, Rua Dr Antonio Bernardino de Almeida, 4200 072 Porto, Portugal Abel Salazar Institute for the Biomedical Sciences ICBAS, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050 313 Porto, Portugal
    Toxicol Sci 142:196-209. 2014
    ..Regarding MTX overall toxicity, an increased risk was associated to SLC19A1 rs7499 G carriers (p = 0.017), SLC46A1 rs2239907 GG (p = 0.030) and, SLCO1B1 rs4149056 T carriers (p = 0.040) and TT (p = 0.019)...
  63. pmc A gold nanoparticle-linked glycoconjugate vaccine against Burkholderia mallei
    Anthony E Gregory
    School of Biosciences, College of Life and Environmental Sciences, University of Exeter, Devon, UK
    Nanomedicine 11:447-56. 2015
    ..AuNPs were covalently coupled with one of three different protein carriers (TetHc, Hcp1 and FliC) followed by conjugation to LPS purified from a non-virulent clonal relative, B. thailandensis...
  64. pmc Orally administered melatonin prevents lipopolysaccharide-induced neural tube defects in mice
    Lin Fu
    Department of Toxicology, Anhui Medical University, Hefei, China Anhui Provincial Key Laboratory of Population Health and Aristogenics, Anhui Medical University, Hefei, China
    PLoS ONE 9:e113763. 2014
    ..showed that maternal LPS exposure significantly down-regulated placental proton-coupled folate transporter (pcft) and disturbed folate transport from maternal circulation through the placentas into the fetus...
  65. pmc Extended loop region of Hcp1 is critical for the assembly and function of type VI secretion system in Burkholderia pseudomallei
    Yan Ting Lim
    1 Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 2 NUS Graduate School of Integrative Sciences and Engineering, National University of Singapore, Singapore 3 Life Sciences Institute, Immunology Program, National University of Singapore, Singapore
    Sci Rep 5:8235. 2015
    ..Our structural study reveals that Hcp1 forms hexameric rings similar to the other Hcp homologs but has an extended loop (Asp40-Arg56) that deviates ..
  66. pmc 6-Substituted Pyrrolo[2,3-d]pyrimidine Thienoyl Regioisomers as Targeted Antifolates for Folate Receptor α and the Proton-Coupled Folate Transporter in Human Tumors
    Lei Wang
    Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, Pennsylvania 15282, United States
    J Med Chem 58:6938-59. 2015
    ..sublines expressing folate receptors (FRs) α or β (IC50s < 1 nM) or the proton-coupled folate transporter (PCFT) (IC50 < 7 nM)...
  67. pmc Identification of an Extracellular Gate for the Proton-coupled Folate Transporter (PCFT-SLC46A1) by Cysteine Cross-linking
    Rongbao Zhao
    From the Departments of Molecular Pharmacology, Medicine
    J Biol Chem 291:8162-72. 2016
    The proton-coupled folate transporter (PCFT, SLC46A1) is required for intestinal folate absorption and folate homeostasis in humans...
  68. pmc Hemolysin coregulated protein 1 as a molecular gluing unit for the assembly of nanoparticle hybrid structures
    Tuan Anh Pham
    Department of Chemistry, Physical Chemistry, University of Konstanz, Universitatstrasse 10, D 78457 Konstanz, Germany
    Beilstein J Nanotechnol 7:351-63. 2016
    ..Here, we use hemolysin coregulated protein 1 (Hcp1) from Pseudomonas aeruginosa as a building and gluing unit for the formation of biohybrid structures by ..
  69. doi Structure, Diversity, and Evolution of a New Family of Soluble Carotenoid-Binding Proteins in Cyanobacteria
    Matthew R Melnicki
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Mol Plant 9:1379-1394. 2016
    ..We solved two crystal structures of the Nostoc sp. PCC 7120 HCP1 protein, each binding a different carotenoid, suggesting that the proteins flexibly bind a range of carotenoids...
  70. pmc Tumor Targeting with Novel 6-Substituted Pyrrolo [2,3-d] Pyrimidine Antifolates with Heteroatom Bridge Substitutions via Cellular Uptake by Folate Receptor α and the Proton-Coupled Folate Transporter and Inhibition of de Novo Purine Nucleotide Biosynthesi
    Lalit K Golani
    Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, Pennsylvania 15282, United States
    J Med Chem 59:7856-76. 2016
    ..Our results establish that heteroatom substitutions in the 3-atom bridge region of 6-substituted pyrrolo[2,3-d]pyrimidines related to 1 provide targeted antifolates that warrant further evaluation as anticancer agents. ..
  71. pmc The proton-coupled folate transporter (PCFT-SLC46A1) and the syndrome of systemic and cerebral folate deficiency of infancy: Hereditary folate malabsorption
    Rongbao Zhao
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, USA Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA
    Mol Aspects Med 53:57-72. 2017
    The proton-coupled folate transporter (PCFT-SLC46A1) is the mechanism by which folates are absorbed across the brush-border membrane of the small intestine...
  72. pmc pH Alkalinization by Chloroquine Suppresses Pathogenic Burkholderia Type 6 Secretion System 1 and Multinucleated Giant Cells
    Jennifer Chua
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA
    Infect Immun 85:. 2017
    ..We show that acid pH upregulates the expression of Hcp1 of T6SS-1 and TssM, a protein coregulated with T6SS-1...
  73. pmc A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus
    Joseph D Mougous
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 312:1526-30. 2006
    ..The apparatus assembled in discrete subcellular locations and exported Hcp1, a hexameric protein that forms rings with a 40 angstrom internal diameter...
  74. pmc Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types
    Nagavedi S Umapathy
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, USA
    Invest Ophthalmol Vis Sci 48:5299-305. 2007
    ..Recently, a new proton-coupled folate transporter (PCFT) in human intestine was reported...
  75. pmc Functional characterization of folate transport proteins in Staten's Seruminstitut rabbit corneal epithelial cell line
    J Jwala
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri Kansas City, 2464 Charlotte Street, Kansas City, MO 64108 2718, USA
    Curr Eye Res 36:404-16. 2011
    ..The overall objective of this study was to investigate and characterize the expression of folate transport proteins in Staten's Seruminstitut rabbit corneal (SIRC) epithelial cell line...
  76. pmc Early stage (IA-IB) primary carcinoma of the fallopian tube: case-control comparison to adenocarcinoma of the ovary
    Charlotte Vaysse
    Department of Surgery, Institut Claudius Regaud, Toulouse, France
    J Gynecol Oncol 22:9-17. 2011
    Early stage primary carcinoma of the fallopian tube (PCFT) is an uncommon condition when strict criteria are applied. The aim of this study was to compare the outcome stage IA-IB PCFT to a matched group of ovarian cancer (OC).
  77. pmc Therapeutic targeting of a novel 6-substituted pyrrolo [2,3-d]pyrimidine thienoyl antifolate to human solid tumors based on selective uptake by the proton-coupled folate transporter
    Sita Kugel Desmoulin
    Graduate Program in Cancer Biology, Wayne State University School of Medicine, Detroit, Michigan, USA
    Mol Pharmacol 80:1096-107. 2011
    The proton-coupled folate transporter (PCFT) is a proton-folate symporter with an acidic pH optimum...
  78. pmc Hcp family proteins secreted via the type VI secretion system coordinately regulate Escherichia coli K1 interaction with human brain microvascular endothelial cells
    Yan Zhou
    Laboratory of Bacterial Pathogenesis, Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Infect Immun 80:1243-51. 2012
    ..Hcp family proteins (the hallmark of T6SS), including Hcp1 and Hcp2, were localized in the bacterial outer membrane, but the involvements of Hcp1 and Hcp2 have been shown to ..
  79. doi Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids
    H Liu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
    Lett Appl Microbiol 55:73-81. 2012
    ..The aims of this study were to create and evaluate the Gateway-compatible plasmids for investigating the function of genes in Vibrio alginolyticus and other Gram-negative bacteria...
  80. pmc The human proton-coupled folate transporter: Biology and therapeutic applications to cancer
    Sita Kugel Desmoulin
    Cancer Biology Graduate Program in Cancer Biology, Department of Oncology, Wayne State University School of Medicine Detroit, MI USA
    Cancer Biol Ther 13:1355-73. 2012
    This review summarizes the biology of the proton-coupled folate transporter (PCFT)...
  81. doi Transcriptional regulation of PCFT by KLF4, HNF4α, CDX2 and C/EBPα: implication in its site-specific expression in the small intestine
    Mai Furumiya
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Nagoya 467 8603, Japan
    Biochem Biophys Res Commun 431:158-63. 2013
    Proton-coupled folate transporter (PCFT), which is responsible for the intestinal uptake of folates and analogs, is expressed only in the proximal region in the small intestine...
  82. doi The monomeric state of the proton-coupled folate transporter represents the functional unit in the plasma membrane
    Phaneendra K Duddempudi
    Department of Pharmacology and Neuroscience, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    FEBS J 280:2900-15. 2013
    ..The proton-coupled folate transporter (PCFT; SLC46A1) has been identified as the major contributor for intestinal folate uptake...
  83. pmc Single nucleotide polymorphisms in CETP, SLC46A1, SLC19A1, CD36, BCMO1, APOA5, and ABCA1 are significant predictors of plasma HDL in healthy adults
    Andrew J Clifford
    Department of Nutrition, University of California, One Shields Avenue, Davis, CA 95616, USA
    Lipids Health Dis 12:66. 2013
    ..We used a linear regression model. Selected genes corresponded to folate metabolism, vitamins B-12, A, and E, and cholesterol pathways or lipid metabolism...
  84. doi Folate transport in mouse cumulus-oocyte complexes and preimplantation embryos
    Megan Kooistra
    Ottawa Hospital Research Institute, Ottawa, Ontario, Canada
    Biol Reprod 89:63. 2013
    ..The mRNAs encoding Folr2 and the intestinal folate transporter Slc46a1 were not detected...
  85. pmc The intestinal absorption of folates
    Michele Visentin
    Departments of Molecular Pharmacology and Medicine, Albert Einstein College of Medicine, Bronx, New York 10461 email
    Annu Rev Physiol 76:251-74. 2014
    ..However, it was only recently that the proton-coupled folate transporter (PCFT) was identified and its critical role in folate transport across the apical brush-border membrane of the proximal ..
  86. pmc A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition
    Victorien Decoin
    LMSM, Laboratoire de Microbiologie Signaux et Microenvironnement, Normandie Université, EA 4312, Universite de Rouen, IUT d Evreux, Evreux, France
    PLoS ONE 9:e89411. 2014
    ..The genome of MFE01 contains at least two hcp genes (hcp1 and hcp2), suggesting that there may be two putative T6SS clusters...
  87. doi Association between reduced folate carrier G80A polymorphism and methotrexate toxicity in childhood acute lymphoblastic leukemia: a meta-analysis
    Hai Rong He
    Department of Pharmacy, the First Affiliated Hospital of Medical College, Xi an Jiaotong University, Xi an, China
    Leuk Lymphoma 55:2793-800. 2014
    ..A recessive model demonstrated no influence of the RFC1 G80A genotype on MTX toxicity. In conclusion, the RFC1 G80A polymorphism does not seem to be a good marker of MTX-related toxicity in pediatric ALL. ..
  88. pmc H-NS regulates the Vibrio parahaemolyticus type VI secretion system 1
    Dor Salomon
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8816, USA
    Microbiology 160:1867-73. 2014
    ..and in two regulators encoded within the T6SS1 gene cluster on expression and secretion of the core T6SS component Hcp1 and on T6SS1-mediated anti-bacterial activity...
  89. pmc Meta-analysis of genome-wide association studies identifies two loci associated with circulating osteoprotegerin levels
    Johnny S H Kwan
    Department of Medicine, Department of Psychiatry and
    Hum Mol Genet 23:6684-93. 2014
    ..Moreover, we estimated that over half of the heritability of OPG levels could be explained by all variants examined in our study. Our findings provide further insight into the genetic regulation of circulating OPG levels. ..
  90. doi Biosensor analysis of anti-citrullinated protein/peptide antibody affinity
    Giada Rossi
    Laboratory of Peptide and Protein Chemistry and Biology, Department of NEUROFARBA, Division of Pharmaceutical Sciences and Nutraceutic, University of Florence, 50019 Florence, Italy
    Anal Biochem 465:96-101. 2014
    ..1 (VCP1) and VCP2 derived from Epstein-Barr virus (EBV)-encoded proteins and histone citrullinated peptide 1 (HCP1) derived from histone H4-were synthesized as tetrameric multiple antigen peptides and immobilized on sensor chips ..
  91. doi Reversible pancytopenia and immunodeficiency in a patient with hereditary folate malabsorption
    Miriam Erlacher
    Department of Pediatrics and Adolescent Medicine, University Medical Center, Freiburg, Germany
    Pediatr Blood Cancer 62:1091-4. 2015
    Mutations in SLC46A1 result in a defect of the proton coupled folate transporter (PCFT) and are the basis of hereditary folate malabsorption (HFM)...
  92. doi Stereoselective recognition of amethopterin enantiomers by the rat proton-coupled folate transporter
    Tomoya Narawa
    School of Pharmacy, Kitasato University
    Biol Pharm Bull 38:545-51. 2015
    ..The observed stereoselectivity of the rPCFT was found to be comparable with that of the human PCFT.
  93. doi Quantitative proteomics unravels that the post-transcriptional regulator Crc modulates the generation of vesicles and secreted virulence determinants of Pseudomonas aeruginosa
    Jose Antonio Reales-Calderón
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Madrid, Spain Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto Ramón y Cajal de Investigaciones Sanitarias IRYCIS, Madrid, Spain
    J Proteomics 127:352-64. 2015
    ..Among them, virulence determinants, such as ToxA, protease IV, azurin, chitin-binding protein, PlcB and Hcp1, were less abundant in the crc-defective mutant...
  94. doi Identification of regulatory mechanisms of intestinal folate transport in condition of folate deficiency
    Shilpa Thakur
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India
    J Nutr Biochem 26:1084-94. 2015
    ..b>proton coupled folate transporter (PCFT) and reduced folate carrier (RFC), which express at the membrane surfaces...
  95. doi Methotrexate influx via folate transporters into alveolar epithelial cell line A549
    Masashi Kawami
    Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Drug Metab Pharmacokinet 30:276-81. 2015
    ..role of two folate transporters in MTX influx, reduced folate carrier (RFC) and proton-coupled folate transporter (PCFT)...
  96. doi Evaluation of proton-coupled folate transporter (SLC46A1) polymorphisms as risk factors for neural tube defects and oral clefts
    Julia E Vandermeer
    Section of Molecular Pathogenesis, Medical Genomics Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, Maryland
    Am J Med Genet A 170:1007-16. 2016
    ..reports have examined the major gene responsible for folate uptake, the proton-coupled folate transporter (SLC46A1). We tested for association between these birth defects and single nucleotide polymorphisms in the SLC46A1 gene...
  97. pmc Targeting Nonsquamous Nonsmall Cell Lung Cancer via the Proton-Coupled Folate Transporter with 6-Substituted Pyrrolo[2,3-d]Pyrimidine Thienoyl Antifolates
    Mike R Wilson
    Department of Oncology M R W, Z H, L P, J K, K W, J H, M R, L H M, and Department of Pharmacology L H M, Wayne State University School of Medicine, Detroit, Michigan Molecular Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Detroit, Michigan Z H, L P, M R, L H M and Division of Medicinal Chemistry, Graduate School of Pharmaceutical Science, Duquesne University, Pittsburgh, Pennsylvania S Y, A G
    Mol Pharmacol 89:425-34. 2016
    ..PMX is transported by the reduced folate carrier (RFC) and proton-coupled folate transporter (PCFT). Unlike RFC, PCFT is active at acidic pH levels characterizing the tumor microenvironment...
  98. ncbi Preliminary evaluation of a scale to assess cognitive function in adults with Down's syndrome: the Prudhoe Cognitive Function Test
    D W K Kay
    Department of Psychiatry, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
    J Intellect Disabil Res 47:155-68. 2003
    ..The aim of the present study was to evaluate the performance of non-demented adults with DS on a subject-directed instrument, the Prudhoe Cognitive Function Test (PCFT), preliminary to its serial use in a prospective study.
  99. doi Folate malabsorption is associated with down-regulation of folate transporter expression and function at colon basolateral membrane in rats
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India
    Br J Nutr 107:800-8. 2012
    ..The decreased uptake was associated with down-regulation of proton-coupled folate transporter (PCFT) and RFC expression at mRNA and protein levels...
  100. doi Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development
    J Cherukad
    Centre for Trophoblast Research, Dept of Physiology, Development and Neuroscience, University of Cambridge, Physiological Laboratories, Downing Street, Cambridge CB2 3EG, UK
    Placenta 33:440-8. 2012
    ..protein expression analysis of folate receptor (Folr), folate transporters (proton-coupled folate receptor [Slc46a1 or PCFT] and reduced folate carrier-1 [Rfc1]) and folate metabolic enzymes (5,10-methylenetetrahydrofolate ..
  101. ncbi Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers
    Ying Yu
    Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Institute of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China
    Arch Microbiol 194:827-35. 2012
    ..However, icmF2 is contained in all strains of both clinical and environmental sources. Deletion of either icmF1 or hcp1 significantly reduced bacterial adhesion to Caco-2 cells or HeLa monolayers...

Research Grants17

  1. ISRAEL DAVID GOLDMAN; Fiscal Year: 2016
    ..This was accomplished with the identification of the "Proton-Coupled Folate Transporter" (PCFT)...
  2. Larry H Matherly; Fiscal Year: 2015
    ....
  3. Larry H Matherly; Fiscal Year: 2016
    ....
  4. Novel Type IV Pili of Enterohemorrhagic E. coli O157:H7
    Jorge Giron; Fiscal Year: 2004
    ..These pili, herein called hemorrhagic coli pili 1 (Hcp1), belong to the virulence-associated type IV pili family...
  5. Ferritin and Iron Nutrition in Health and Disease
    Elizabeth Theil; Fiscal Year: 2005
    ..The results will clarify mechanisms of ferritin iron uptake and characterize molecular genetic differences in iron uptake for improving dietary iron sources in health and disease. ..
  6. Quantitation of In Vivo Human Lutein Metabolism
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010
    ..Therefore, the purpose of this study is to determine how the human body uses lutein and other related compounds. ..
  7. DYNAMIC AND KINETIC BEHAVIOR OF FOLATE METABOLISM
    Andrew Clifford; Fiscal Year: 2005
    ..We will determine the in vivo kinetic behavior of a tracer dose of folate in blood, urine, and stool. We will then compare the behavior of folate metabolism (functional endpoint) among the various genotypes. ..
  8. IN VIVO DYNAMIC OF BETA-CAROTENE METABOLISM IN HUMANS
    Andrew Clifford; Fiscal Year: 2006
    ....
  9. Intestinal Iron Transport in Iron Deficiency/Anemia
    James Collins; Fiscal Year: 2005
    ..abstract_text> ..
  10. Quantitation of Tocopherol Metabolism in Humans
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010
    ..Particular attention will be directed to the absorption phase. The results are expected to provide information about vitamin E biopotency and eventually improved Dietary Recommendations. ..
  11. MOLECULAR CORRELATES OF METHOTREXATE IN CHILDHOOD ALL
    Larry Matherly; Fiscal Year: 2007
    ..abstract_text> ..
  12. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
    ....
  13. Mechanism/regulation of human intestinal thiamin uptake
    HAMID SAID; Fiscal Year: 2008
    ..This should ultimately assist us in the designing of effective strategies to optimize thiamin body homeostasis in conditions associated with thiamin deficiency and sub-optimal levels. ..
  14. C elegans:a model to study intestinal water-soluble vitamins transport in vivo
    HAMID SAID; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  15. IRON & OXYGEN BIOLOGY IN SCD, HH & BETA-THAL MICE
    Elizabeth Theil; Fiscal Year: 2002
    ..The resulting information will be used to help probe the efficiency of presently available and experimental chelators, not only in promoting iron excretion but also in the prevention of defined types of cellular damage. ..
  16. Workshop on BioIron in Thalassemia, Sickle Cell Disease and Hemochromatosis
    Elizabeth Theil; Fiscal Year: 2004
    ..Fuller understanding of iron homeostasis in Thalassemia, Sickle Cell Disease and Hemochromatosis and improved disease management strategies can be expected. ..
  17. Workshop on Biolron in Thalassemia & Sickle Cell Disease
    Elizabeth Theil; Fiscal Year: 2002
    ..abstract_text> ..