Skp1

Summary

Gene Symbol: Skp1
Description: S-phase kinase associated protein 1
Alias: EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A, S-phase kinase-associated protein 1, OCP-2, RNA polymerase II elongation factor-like protein OCP2, SIII, cyclin A/CDK2-associated p19, cyclin-A/CDK2-associated protein p19, organ of Corti protein 2, organ of Corti protein II, p19skp1, transcription elongation factor B polypeptide 1-like
Species: human

Top Publications

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
  2. pmc Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy
    M D Gomes
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 98:14440-5. 2001
  3. ncbi Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation
    A Marti
    Friedrich Miescher Institut, Basel, Switzerland
    Nat Cell Biol 1:14-9. 1999
  4. ncbi The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes
    Lakshmanan Ganesh
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nature 426:853-7. 2003
  5. pmc Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice
    Takeya Tsutsumi
    Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
    Mol Cell Biol 28:743-51. 2008
  6. ncbi SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma
    Vanesa Fernández-Sáiz
    Department of Medicine III, Klinikum rechts der Isar, Technische Universitat Munchen, Ismaninger Strasse 22, 81675 Munich, Germany
    Nat Cell Biol 15:72-81. 2013
  7. ncbi Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A
    J J Michel
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 27599 7295, USA
    Cell Growth Differ 9:435-49. 1998
  8. pmc Human CUL-1 associates with the SKP1/SKP2 complex and regulates p21(CIP1/WAF1) and cyclin D proteins
    Z K Yu
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:11324-9. 1998
  9. pmc The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro
    J T Winston
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 13:270-83. 1999
  10. ncbi The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin
    E Latres
    Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, NY 10016, USA
    Oncogene 18:849-54. 1999

Research Grants

  1. Yi Sun; Fiscal Year: 2016
  2. B-RAF Regulation of the Cell Cycle in Melanoma
    Andrew E Aplin; Fiscal Year: 2013
  3. Yi Sun; Fiscal Year: 2016
  4. Yi Sun; Fiscal Year: 2014
  5. Regulation of Cellular Growth and Division by Ubiquitin-Mediated Proteolysis
    Jennifer A Benanti; Fiscal Year: 2012
  6. Yi Sun; Fiscal Year: 2015
  7. Role of the SCF/FBX011 ubiquitin ligase in the control of cell proliferation
    Michele Pagano; Fiscal Year: 2013
  8. Role of the SCF/Fbxo15 ubiquitin ligase in stem cells and cancer biology
    Michele Pagano; Fiscal Year: 2012
  9. Jason A Carlyon; Fiscal Year: 2014
  10. MECHANISMS OF CELL CYCLE CONTROL DURING MAMMARY GLAND DE
    Hui Zhang; Fiscal Year: 2001

Detail Information

Publications215 found, 100 shown here

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
    ..We report here that the IkappaBalpha-ubiquitin ligase (IkappaBalpha-E3) is an SCF-like complex containing Skp1, cullin-1, and two homologous F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2...
  2. pmc Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy
    M D Gomes
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 98:14440-5. 2001
    ..It contains a functional F-box domain that binds to Skp1 and thereby to Roc1 and Cul1, the other components of SCF-type Ub-protein ligases (E3s), as well as a nuclear ..
  3. ncbi Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation
    A Marti
    Friedrich Miescher Institut, Basel, Switzerland
    Nat Cell Biol 1:14-9. 1999
    ....
  4. ncbi The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes
    Lakshmanan Ganesh
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nature 426:853-7. 2003
    ..Through its effects on the proteasome, Murr1 acts as a genetic restriction factor that inhibits HIV-1 replication in lymphocytes, which could contribute to the regulation of asymptomatic HIV infection and the progression of AIDS...
  5. pmc Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice
    Takeya Tsutsumi
    Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
    Mol Cell Biol 28:743-51. 2008
    CUL7 binds to SKP1, RBX1, and FBXW8 to form a cullin-RING ligase, or an SKP1-cullin-F box protein complex. The targeted disruption of the Cul7 gene in mice results in significant reduction in embryo size and neonatal lethality...
  6. ncbi SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma
    Vanesa Fernández-Sáiz
    Department of Medicine III, Klinikum rechts der Isar, Technische Universitat Munchen, Ismaninger Strasse 22, 81675 Munich, Germany
    Nat Cell Biol 15:72-81. 2013
    ....
  7. ncbi Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A
    J J Michel
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 27599 7295, USA
    Cell Growth Differ 9:435-49. 1998
    The budding yeast gene product, CDC53p, forms E3-like SCF complexes with SKP1 and F-box-containing proteins to mediate the ubiquitin-dependent degradation of G1 cyclins and cyclin-dependent kinase (CDK) inhibitors...
  8. pmc Human CUL-1 associates with the SKP1/SKP2 complex and regulates p21(CIP1/WAF1) and cyclin D proteins
    Z K Yu
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:11324-9. 1998
    ..In many transformed cells, the cyclin A/CDK2 complex that contains S-phase kinase associated proteins 1 and 2 (SKP1 and SKP2) is highly induced...
  9. pmc The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro
    J T Winston
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 13:270-83. 1999
    ..by the recently described SCF complexes, the archetype of which was first described in budding yeast and contains Skp1, Cdc53, and the F-box protein Cdc4...
  10. ncbi The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin
    E Latres
    Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, NY 10016, USA
    Oncogene 18:849-54. 1999
    ..the proteolysis of several G1 regulators is controlled by ubiquitin ligases (or SCFs) formed by three subunits: Skp1, Cul A (Cdc53), and one of many F-box proteins...
  11. ncbi SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27
    A C Carrano
    Department of Pathology, New York University Medical Center, New York 10016, USA
    Nat Cell Biol 1:193-9. 1999
    ..Thus, p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation...
  12. ncbi Homodimer of two F-box proteins betaTrCP1 or betaTrCP2 binds to IkappaBalpha for signal-dependent ubiquitination
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Company Ltd, 21 Miyukigaoka, Tsukuba shi, Ibaraki, 305 8585, Japan
    J Biol Chem 275:2877-84. 2000
    ..These results indicate that not only betaTrCP1 but also betaTrCP2 participates in the ubiquitination-dependent destruction of IkappaBalpha by forming SCF(betaTrCP1-betaTrCP1) and SCF(betaTrCP2-betaTrCP2) ubiquitin-ligase complexes...
  13. pmc Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha
    M A Read
    LeukoSite, Inc, Cambridge, Massachusetts 02139, USA
    Mol Cell Biol 20:2326-33. 2000
    ..These observations provide a functional link between the highly related ubiquitin and Nedd8 pathways of protein modification and show how they operate together to selectively target the signal-dependent degradation of IkappaBalpha...
  14. ncbi Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex
    B A Schulman
    Cellular Biochemistry and Biophysics Program, Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 408:381-6. 2000
    ..F-box proteins are the substrate-recognition components of SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligases...
  15. ncbi Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome
    S Lyapina
    Department of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 292:1382-5. 2001
    ....
  16. ncbi Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses
    S I Matsuzawa
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 7:915-26. 2001
    ..were identified in which Siah is physically linked to Ebi by association with a novel Sgt1 homolog SIP that binds Skp1, a central component of Skp1-Cullin-F box complexes...
  17. pmc NEDD8 recruits E2-ubiquitin to SCF E3 ligase
    T Kawakami
    Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    EMBO J 20:4003-12. 2001
    ..NEDD8 is known to enhance the ubiquitylating activity of the SCF complex (composed of Skp1, Cul-1, ROC1 and F-box protein), but the mechanistic role is largely unknown...
  18. ncbi The novel human DNA helicase hFBH1 is an F-box protein
    Jaehoon Kim
    National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Chunchun dong, Changan Ku, Suwon, Kyunggi Do, 440 746, Korea
    J Biol Chem 277:24530-7. 2002
    ..The hFBH1 enzyme interacted with human SKP1 and formed an SCF (SKP1/Cullin/F-box) complex together with human Cullin and ROC1...
  19. ncbi Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex
    Ning Zheng
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    Nature 416:703-9. 2002
    ..Here we present the crystal structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF complex, which shows that Cul1 is an elongated protein that consists of a long stalk and a globular ..
  20. pmc Dual mode of degradation of Cdc25 A phosphatase
    Maddalena Donzelli
    European Institute of Oncology, 435 Via Ripamonti, I 20141 Milan, Italy
    EMBO J 21:4875-84. 2002
    ..Moreover, SCF (Skp1/Cullin/F-box) inactivation using an interfering Cul1 mutant accumulates and stabilizes Cdc25 A...
  21. pmc CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex
    Dora C Dias
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 99:16601-6. 2002
    ..It is widely accepted that an F-box protein directs substrate ubiquitination within a Skp1.CUL1.F-box protein.ROC1 (SCF-ROC1) E3 ubiquitin ligase complex...
  22. ncbi CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex
    Jianyu Zheng
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, Yale University, New Haven, CT 06520, USA
    Mol Cell 10:1519-26. 2002
    ..We found the majority of CUL1 is in a complex with CAND1 and ROC1 independent of SKP1 and F box protein SKP2...
  23. ncbi TIP120A associates with cullins and modulates ubiquitin ligase activity
    Kyoeng Woo Min
    Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul 120 749, Korea
    J Biol Chem 278:15905-10. 2003
    ..TIP120A formed a complex with CUL1 and Rbx1, but interfered with the binding of Skp1 and F-box proteins to CUL1...
  24. ncbi Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC
    Nagi G Ayad
    Department of Cell Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 113:101-13. 2003
    ..Degradation of Tome-1 during G1 allows for wee 1 accumulation during interphase, thereby providing a critical link between the APC and SCF pathways in regulation of cdk1/cyclin B activity and thus mitotic entry and exit...
  25. ncbi Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1
    Kiyotaka Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Japan
    Biochem Biophys Res Commun 303:1209-16. 2003
    The SCF complex, which consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, functions as an E3 ubiquitin ligase...
  26. ncbi Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins
    Marcin Nowotny
    Nencki Institute of Experimental Biology, 3 Pasteur Street, 02 093 Warsaw, Poland
    J Biol Chem 278:26923-8. 2003
    ..A recently discovered yeast homolog of CacyBP/SIP, Sgt1, associates with Skp1 and regulates its function in the Skp1/Cullin1/F-box complex ubiquitin ligase and in kinetochore complexes...
  27. ncbi Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
    Geng Wu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:1445-56. 2003
    ..The 3.0 A structure of a beta-TrCP1-Skp1-beta-catenin complex reveals the basis of substrate recognition by the beta-TrCP1 WD40 domain...
  28. pmc Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis
    Takehiro Arai
    Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:9855-60. 2003
    ..We demonstrate that p185 forms an SCF-like complex with Skp1, Rbx1, Fbw6 (Fbx29), and FAP68 (FAP48, glomulin)...
  29. pmc M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:4419-24. 2004
    ..study, we have identified the beta-transducin repeat-containing protein 1/2 (beta-TrCP1/2) F-box protein-containing SKP1/Cul1/F-box protein (SCF) complex (SCF(beta-TrCP1/2)) as an E3 ubiquitin ligase for Wee1A ubiquitination...
  30. ncbi FBW2 targets GCMa to the ubiquitin-proteasome degradation system
    Chih Sheng Yang
    Graduate Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan
    J Biol Chem 280:10083-90. 2005
    ..We found that SKP1 and CUL1, two key components of the SCF complex, associate with hGCMa in vivo...
  31. ncbi The modular structure of SIP facilitates its role in stabilizing multiprotein assemblies
    Shibani Bhattacharya
    Department of Biochemistry, Center for Structural Biology, 5140 BIOSCI MRBIII, Vanderbilt University, Nashville, Tennessee 37232 8725, USA
    Biochemistry 44:9462-71. 2005
    ..SIP) was identified as a novel adaptor that physically links the E3 ubiquitin ligase activity of Siah-1 with Skp1 and Ebi F-Box protein in the degradation of beta-catenin, a transcriptional activator of TCF/LEF genes...
  32. ncbi NIPA defines an SCF-type mammalian E3 ligase that regulates mitotic entry
    Florian Bassermann
    Department of Internal Medicine III, Technical University of Munich, 81675 Munich, Germany
    Cell 122:45-57. 2005
    ..Thus, SCF(NIPA)-based ubiquitination may regulate S-phase completion and mitotic entry in the mammalian cell cycle...
  33. ncbi FBXO31 is the chromosome 16q24.3 senescence gene, a candidate breast tumor suppressor, and a component of an SCF complex
    Raman Kumar
    Breast Cancer Genetics Group, Dame Roma Mitchell Cancer Research Laboratories, Department of Medicine, University of Adelaide and Hanson Institute, Institute of Medical and Veterinary Science Adelaide, Australia
    Cancer Res 65:11304-13. 2005
    ..FBXO31 contains an F-box domain and is associated with the proteins Skp1, Roc-1, and Cullin-1, suggesting that FBXO31 is a component of a SCF ubiquitination complex...
  34. ncbi 17-AAG, an Hsp90 inhibitor, causes kinetochore defects: a novel mechanism by which 17-AAG inhibits cell proliferation
    Y Niikura
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    Oncogene 25:4133-46. 2006
    ..We found that HSP90 associates with SGT1 (suppressor of G2 allele of skp1; SUGT1) in human cells and that depletion of SGT1 sensitizes HeLa cells to 17-AAG...
  35. pmc Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex
    Douglas I Lin
    The Leonard and Madlyn Abramson Family Cancer Research Institute and Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell 24:355-66. 2006
    ..We demonstrate that Thr286-phosphorylated cyclin D1 is recognized by a Skp1-Cul1-F box (SCF) ubiquitin ligase where FBX4 and alphaB crystallin govern substrate specificity...
  36. pmc Phosphorylation of Skp2 regulated by CDK2 and Cdc14B protects it from degradation by APC(Cdh1) in G1 phase
    Geneviève Rodier
    Institut de Recherche en Immunologie et Cancérologie, Universite de Montreal, Montreal, Quebec, Canada
    EMBO J 27:679-91. 2008
    ..Thus, our results reveal that reversible phosphorylation plays a key role in the timing of Skp2 expression in the cell cycle...
  37. pmc Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progression
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:65-78. 2008
    ..We conclude that tight regulation of the Bora protein by its synthesis and degradation is critical for cell cycle progression...
  38. pmc Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation
    David M Duda
    Howard Hughes Medical Institute, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell 134:995-1006. 2008
    ..Thus, our results point to a conformational control of CRL activity, with ligation of NEDD8 shifting equilibria to disfavor inactive CAND1-bound closed architectures, and favor dynamic, open forms that promote polyubiquitination...
  39. pmc Structural and functional coupling of Hsp90- and Sgt1-centred multi-protein complexes
    Minghao Zhang
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 27:2789-98. 2008
    ....
  40. pmc Phosphorylation-dependent regulation of cytosolic localization and oncogenic function of Skp2 by Akt/PKB
    Hui Kuan Lin
    Department of Pathology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Cell Biol 11:420-32. 2009
    Skp2 is an F-box protein that forms the SCF complex with Skp1 and Cullin-1 to constitute an E3 ligase for ubiquitylation...
  41. pmc Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development
    Toru Saiga
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Mol Cell Biol 29:3529-43. 2009
    ..In addition, neuronal migration was impaired in Fbxo45(-)(/)(-) mice. These results suggest that Fbxo45 forms a novel Fbxo45-PAM ubiquitin ligase complex that plays an important role in neural development...
  42. pmc Control of iron homeostasis by an iron-regulated ubiquitin ligase
    Ajay A Vashisht
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Science 326:718-21. 2009
    ..We found that a SKP1-CUL1-FBXL5 ubiquitin ligase protein complex associates with and promotes the iron-dependent ubiquitination and ..
  43. pmc An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis
    Ameen A Salahudeen
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 326:722-6. 2009
    ..These observations suggest a mechanistic link between iron sensing via the FBXL5 hemerythrin domain, IRP2 regulation, and cellular responses to maintain mammalian iron homeostasis...
  44. pmc Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates
    Gary Kleiger
    Howard Hughes Medical Institute and the Division of Biology, 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 139:957-68. 2009
    ..We discuss different strategies by which processive ubiquitin chain synthesis may be achieved...
  45. pmc Fbxo45, a novel ubiquitin ligase, regulates synaptic activity
    Hirobumi Tada
    Department of Physiology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 285:3840-9. 2010
    ..We also found that Fbxo45 induces the degradation of a synaptic vesicle-priming factor, Munc13-1. We propose that Fbxo45 plays an important role in the regulation of neurotransmission by modulating Munc13-1 at the synapse...
  46. pmc Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa
    Christopher T Price
    Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
    PLoS Pathog 5:e1000704. 2009
    ..expressed AnkB within mammalian cells is localized to the periphery of the cell where it co-localizes with host SKP1 and recruits polyubiquitinated proteins, which results in restoration of intracellular growth to the ankB mutant ..
  47. pmc Phosphorylation of Ser72 is dispensable for Skp2 assembly into an active SCF ubiquitin ligase and its subcellular localization
    Tarig Bashir
    Department of Pathology, New York University Cancer Institute, Smilow Research Center, New York University School of Medicine, New York, NY, USA
    Cell Cycle 9:971-4. 2010
    F-box proteins are the substrate recognition subunits of SCF (Skp1, Cul1, F-box protein) ubiquitin ligase complexes. Skp2 is a nuclear F-box protein that targets the CDK inhibitor p27 for ubiquitin- and proteasome-dependent degradation...
  48. pmc Structural basis of dimerization-dependent ubiquitination by the SCF(Fbx4) ubiquitin ligase
    Yunfeng Li
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Biol Chem 285:13896-906. 2010
    The F-box proteins are the substrate recognition subunits of the SCF (Skp1-Cul1-Rbx1-F- box protein) ubiquitin ligase complexes that control the stability of numerous regulators in eukaryotic cells...
  49. pmc SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation
    Vincenzo D'Angiolella
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 522 First Avenue, SRB 1107, New York, New York 10016, USA
    Nature 466:138-42. 2010
    Generally, F-box proteins are the substrate recognition subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase complexes, which mediate the timely proteolysis of important eukaryotic regulatory proteins...
  50. pmc An OBSL1-Cul7Fbxw8 ubiquitin ligase signaling mechanism regulates Golgi morphology and dendrite patterning
    Nadia Litterman
    Department of Pathology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Biol 9:e1001060. 2011
    ....
  51. doi Skp1 stabilizes the conformation of F-box proteins
    Yukiko Yoshida
    Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    Biochem Biophys Res Commun 410:24-8. 2011
    The SCF ubiquitin ligase complex consists of four components, Skp1, Cul1, ROC1/Rbx1, and a variable subunit F-box protein, which serves as a receptor for target proteins...
  52. pmc The SKP1-Cul1-F-box and leucine-rich repeat protein 4 (SCF-FbxL4) ubiquitin ligase regulates lysine demethylase 4A (KDM4A)/Jumonji domain-containing 2A (JMJD2A) protein
    Capucine Van Rechem
    Massachusetts General Hospital Cancer Center and Department of Medicine, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    J Biol Chem 286:30462-70. 2011
    ..JMJD2A turnover is coordinated through the SKP1-Cul1-F-box ubiquitin ligase complex that contains cullin 1 and the F-box and leucine-rich repeat protein 4 (FbxL4)...
  53. pmc SCF β-TRCP targets MTSS1 for ubiquitination-mediated destruction to regulate cancer cell proliferation and migration
    Jiateng Zhong
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA
    Oncotarget 4:2339-53. 2013
    ..It further suggests that preventing MTSS1 degradation could be a possible novel strategy for clinical treatment of more aggressive breast and prostate cancers...
  54. pmc SCF(β-TRCP)-mediated degradation of NEDD4 inhibits tumorigenesis through modulating the PTEN/Akt signaling pathway
    Jia Liu
    Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Life Science, FIST, Xi an Jiaotong University, Xi an, China
    Oncotarget 5:1026-37. 2014
    ....
  55. ncbi SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
    C Bai
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Cell 86:263-74. 1996
    We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F-binding protein...
  56. pmc Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway
    J Lisztwan
    Friedrich Miescher Institut, Basel, Switzerland
    EMBO J 17:368-83. 1998
    ..and transformed cells, the F-box protein p45(SKP2) is required for S phase and forms stable complexes with p19(SKP1) and cyclin A-cyclin-dependent kinase (CDK)2...
  57. pmc Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein
    S A Lyapina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 95:7451-6. 1998
    ....
  58. ncbi A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
    F Margottin
    CJF 97 03 INSERM, Institut Cochin de Genetique Moleculaire, Universite Paris V, Faculté de Médecine Cochin, France
    Mol Cell 1:565-74. 1998
    ..These data suggest that beta TrCP and Skp1p represent components of a novel ER-associated protein degradation pathway that mediates CD4 proteolysis...
  59. ncbi NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases
    Jidong Liu
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 10:1511-8. 2002
    ..CAND1 formed a ternary complex with CUL1 and ROC1. CAND1 dissociated SKP1 from CUL1 and inhibited SCF ligase activity in vitro...
  60. pmc SCFhFBH1 can act as helicase and E3 ubiquitin ligase
    Jeong Hoon Kim
    National Creative Research Initiative Center for Cell Cycle Control, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejoen, 305 701, Korea
    Nucleic Acids Res 32:2287-97. 2004
    In our previous study, we found that a human F-box DNA helicase, named hFBH1, interacted with SKP1 to form an SCF (SKP1-Cul1-F-box protein) complex together with CUL1 and ROC1 in an F-box-dependent manner...
  61. pmc Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1
    David V Hansen
    Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
    Mol Biol Cell 15:5623-34. 2004
    ..These data support the hypothesis that Plk1 activates the APC by directing the SCF-dependent destruction of Emi1 in prophase...
  62. ncbi Multisite phosphorylation of nuclear interaction partner of ALK (NIPA) at G2/M involves cyclin B1/Cdk1
    Florian Bassermann
    Department of Internal Medicine III, Technical University of Munich, 81675 Munich, Germany, and Department of Pathology, St Jude Chidren s Research Hospital, Memphis, TN 38105, USA
    J Biol Chem 282:15965-72. 2007
    Nuclear interaction partner of ALK (NIPA) is an F-box-containing protein that defines a nuclear skp1 cullin F-box (SCF)-type ubiquitin E3 ligase (SCFNIPA) implicated in the regulation of mitotic entry...
  63. ncbi SCFFbxl3 controls the oscillation of the circadian clock by directing the degradation of cryptochrome proteins
    Luca Busino
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, NY 10016, USA
    Science 316:900-4. 2007
    ..Silencing of Fbxl3 produced no effect in Cry1-/-;Cry2-/- cells, which shows that Fbxl3 controls clock oscillations by mediating the degradation of CRY proteins...
  64. pmc Regulation of neddylation and deneddylation of cullin1 in SCFSkp2 ubiquitin ligase by F-box protein and substrate
    Gil Bornstein
    Unit of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 103:11515-20. 2006
    ..We found that the supplementation of Skp2-Skp1 and substrate (along with further components necessary for substrate presentation to the ubiquitin ligase) to ..
  65. pmc Evidence for the direct involvement of {beta}TrCP in Gli3 protein processing
    Baolin Wang
    Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, W404, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:33-8. 2006
    ..Ci processing also requires Slimb, an F-box protein of SCF (Skp1/Cullin/F-box proteins) complex, and the proteasome, but the interplay between phosphorylation and the activity of ..
  66. ncbi COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis
    Alessandra Devoto
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK, and John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK
    Plant J 32:457-66. 2002
    ..Significantly, each of these responses requires COI1, an F-box protein. Other F-box proteins interact with SKP1 and cullin proteins to form SCF complexes that selectively recruit regulatory proteins targeted for ubiquitination...
  67. ncbi Human delta-lactoferrin is a transcription factor that enhances Skp1 (S-phase kinase-associated protein) gene expression
    Christophe Mariller
    Unite de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576 CNRS Université des Sciences et Technologies de Lille1, Villeneuve d Ascq, France
    FEBS J 274:2038-53. 2007
    ..Using macroarrays, the expression of genes involved in the G(1)/S transition was examined. Among these, Skp1 showed 2-3-fold increased expression at both the mRNA and protein levels...
  68. ncbi A novel F-box protein involved in sexual development and pathogenesis in Gibberella zeae
    You Kyoung Han
    School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151 921, Korea
    Mol Microbiol 63:768-79. 2007
    ..is similar to fungal F-box proteins including Saccharomyces cerevisiae Grr1, a well-characterized component of the Skp1-Cullin-F-box protein (SCF(Grr1)) E3 ligase complex required for protein degradation. FBP1 also can bind both S...
  69. pmc Ectromelia virus encodes a novel family of F-box proteins that interact with the SCF complex
    Nick van Buuren
    621 HMRC, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada
    J Virol 82:9917-27. 2008
    ..These observations suggested that the ectromelia virus F-box proteins interact with SCF (Skp1, cullin-1, and F-box) ubiquitin ligases...
  70. pmc Disruption of Sag/Rbx2/Roc2 induces radiosensitization by increasing ROS levels and blocking NF-kappaB activation in mouse embryonic stem cells
    Mingjia Tan
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Free Radic Biol Med 49:976-83. 2010
    ..with antioxidant activity when acting alone or E3 ligase activity when complexed with other components of SCF (Skp1, cullins, F-box proteins) E3 ubiquitin ligases...
  71. pmc Saccharomyces cerevisiae ubiquitin-like protein Rub1 conjugates to cullin proteins Rtt101 and Cul3 in vivo
    Jose M Laplaza
    Section of Molecular and Cellular Biology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Biochem J 377:459-67. 2004
    ..the cullin protein Cdc53p (cell division cycle 53 protein), a subunit of a class of ubiquitin E3 ligases named SCF (Skp1-Cdc53-F-box protein) complex...
  72. ncbi Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors
    D S Chiaur
    Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    Cytogenet Cell Genet 88:255-8. 2000
    ..SCF complexes (formed by Skp1, cullin, and one of many Fbps) act as protein-ubiquitin ligases that control the G(1)/S transition of the ..
  73. pmc SCF E3 ubiquitin ligases as anticancer targets
    L Jia
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 4424B Medical Science I, 1301 Catherine Street, Ann Arbor, MI 48109, USA
    Curr Cancer Drug Targets 11:347-56. 2011
    The SCF multisubunit complex (Skp1, Cullins, F-box proteins) E3 ubiquitin ligase, also known as CRL (Cullin-RING ubiquitin Ligase) is the largest E3 ubiquitin ligase family that promotes the ubiquitination of various regulatory proteins ..
  74. pmc The orthopoxvirus 68-kilodalton ankyrin-like protein is essential for DNA replication and complete gene expression of modified vaccinia virus Ankara in nonpermissive human and murine cells
    Karin M Sperling
    Division of Virology, Paul Ehrlich Institut, Langen, Germany
    J Virol 83:6029-38. 2009
    ..repeats and an F-box-like domain and forms an SCF ubiquitin ligase complex together with the cellular proteins Skp1a and Cullin-1...
  75. ncbi Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases
    Manabu Furukawa
    Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Nat Cell Biol 5:1001-7. 2003
    ..Cul1 and Cul7, but not other cullins, bind to an adaptor protein, Skp1. Cul1 associates with one of many F-box proteins through Skp1 to assemble various SCF-Roc1 E3 ligases that each ..
  76. pmc SAG/ROC2 E3 ligase regulates skin carcinogenesis by stage-dependent targeting of c-Jun/AP1 and IkappaB-alpha/NF-kappaB
    Qingyang Gu
    Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI 48109, USA
    J Cell Biol 178:1009-23. 2007
    Sensitive to apoptosis gene (SAG)/regulator of cullins-2-Skp1-cullin-F-box protein (SCF) E3 ubiquitin ligase regulates cellular functions through ubiquitination and degradation of protein substrates...
  77. ncbi The Caenorhabditis elegans Skp1-related gene family: diverse functions in cell proliferation, morphogenesis, and meiosis
    Sudhir Nayak
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Biol 12:277-87. 2002
    ..A key SCF component is the Skp1 protein that functions within the complex to link the substrate-recognition subunit to a cullin that in turn binds ..
  78. ncbi Cytoplasmic glycosylation of protein-hydroxyproline and its relationship to other glycosylation pathways
    Christopher M West
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Biochim Biophys Acta 1673:29-44. 2004
    The Skp1 protein, best known as a subunit of E3(SCF)-ubiquitin ligases, is subject to complex glycosylation in the cytoplasm of the cellular slime mold Dictyostelium...
  79. doi The Skp1-like protein SSK1 is required for cross-pollen compatibility in S-RNase-based self-incompatibility
    Lan Zhao
    Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing 100190, China
    Plant J 62:52-63. 2010
    ..Here we report that PhSSK1 (Petunia hybrida SLF-interacting Skp1-like1), an equivalent of AhSSK1 of Antirrhinum hispanicum, is expressed specifically in pollen and acts as an ..
  80. ncbi Multiple Skp1-related proteins in Caenorhabditis elegans: diverse patterns of interaction with Cullins and F-box proteins
    Atsushi Yamanaka
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, 812 8582, Fukuoka, Japan
    Curr Biol 12:267-75. 2002
    ..In yeast and mammals, the SCF complex consists of common components, including Skp1, Cdc53/Cul1, and Rbx1, as well as variable components known as F-box proteins...
  81. ncbi Specificity of a soluble UDP-galactose: fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium
    Catherine Ketcham
    Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida 32610 0235, USA
    J Biol Chem 279:29050-9. 2004
    b>Skp1 is an adaptor-like protein in E3(SCF)-ubiquitin ligases and other multiprotein complexes of the cytoplasm and nucleus...
  82. ncbi Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor
    K G Suresh Kumar
    Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:46614-20. 2004
    ..interferon alpha receptor 1) subunit of the type I interferon (IFN) receptor is mediated by the SCFbeta-Trcp (Skp1-Cullin1-F-box protein beta transducin repeat-containing protein) E3 ubiquitin ligase in a phosphorylation-dependent ..
  83. ncbi The Skp1 prolyl hydroxylase from Dictyostelium is related to the hypoxia-inducible factor-alpha class of animal prolyl 4-hydroxylases
    Hanke van der Wel
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 280:14645-55. 2005
    b>Skp1 is a cytoplasmic and nuclear protein of eukaryotes best known as an adaptor in SCF ubiquitin-protein isopeptide ligases...
  84. pmc Identification of a conserved F-box protein 6 interactor essential for endocytosis and cytokinesis in fission yeast
    Isabelle Jourdain
    Cancer Research UK, London Research Institute, UK
    Biochem J 420:169-77. 2009
    The F-box domain is a degenerated motif consisting of approximately 40 amino acid residues that specifically bind Skp1, a core component of the SCF (Skp1-Cdc53/Cullin 1-F-box protein) ubiquitin ligase...
  85. pmc RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27
    Mingjia Tan
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 4424B MS I, 1301 Catherine Street, Ann Arbor, MI 48109 5936, USA
    Proc Natl Acad Sci U S A 106:6203-8. 2009
    RBX1 (RING box protein-1) or ROC1 (regulator of cullins-1) is the RING component of SCF (Skp1, Cullins, F-box proteins) E3 ubiquitin ligases, which regulate diverse cellular processes by targeting various substrates for degradation...
  86. pmc RBX1/ROC1-SCF E3 ubiquitin ligase is required for mouse embryogenesis and cancer cell survival
    Lijun Jia
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 4424B Medical Science I, 1301 Catherine Street, Ann Arbor, MI 48109, USA
    Cell Div 4:16. 2009
    RBX1 (also known as ROC1) is a RING subunit of SCF (Skp1, Cullins, F-box proteins) E3 ubiquitin ligases, required for SCF to direct a timely degradation of diverse substrates, thereby regulating numerous cellular processes under both ..
  87. pmc Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex
    K Iwai
    Department of Molecular Biology, Graduate School of Biostudies, Kyoto University, Kyoto, 606 8501, Japan
    Proc Natl Acad Sci U S A 96:12436-41. 1999
    ..In light of the structural analogy of VBC-CUL-2 to SKP1-CUL-1-F-box ubiquitin ligases, the ubiquitin ligase activity of VBC-CUL-2 was examined in this study...
  88. pmc Null mutation of AtCUL1 causes arrest in early embryogenesis in Arabidopsis
    Wen Hui Shen
    Institut de Biologie Moleculaire des Plantes du CNRS, 67084 Strasbourg, France
    Mol Biol Cell 13:1916-28. 2002
    The SCF (for SKP1, Cullin/CDC53, F-box protein) ubiquitin ligase targets a number of cell cycle regulators, transcription factors, and other proteins for degradation in yeast and mammalian cells...
  89. pmc The F-box gene family is expanded in herbaceous annual plants relative to woody perennial plants
    Xiaohan Yang
    Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA
    Plant Physiol 148:1189-200. 2008
    F-box proteins are generally responsible for substrate recognition in the Skp1-Cullin-F-box complexes that are involved in protein degradation via the ubiquitin-26S proteasome pathway...
  90. doi A truncated two-alpha-helix F-box present in poxvirus ankyrin-repeat proteins is sufficient for binding the SCF1 ubiquitin ligase complex
    Stephanie Sonnberg
    Department of Microbiology and Immunology, University of Otago, Dunedin 9016, New Zealand
    J Gen Virol 90:1224-8. 2009
    ..proteins, most of which contain an F-box-like motif necessary for the interaction of the ANK proteins with SCF1 (Skp1-Cullin1-F-box) complexes. The viral motif is generally truncated compared with the three-alpha-helix cellular F-box...
  91. pmc Primate lentiviral virion infectivity factors are substrate receptors that assemble with cullin 5-E3 ligase through a HCCH motif to suppress APOBEC3G
    Kun Luo
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:11444-9. 2005
    ..F-box- and BC-box-containing receptors use Skp1, ElonginB, and ElonginC as adaptors to recruit Cul1/Cul7 and Cul2/Cul5, respectively...
  92. pmc The human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination
    Yun Seok Choi
    Division of Magnetic Resonance, Korea Basic Science Institute Ochang Campus, Cheongwon Gun, Ochang eup, Yangcheong Ri 804 1, Chungcheongbuk Do 363 883, South Korea
    J Biol Chem 285:17754-62. 2010
    Cdc34 is an E2 ubiquitin-conjugating enzyme that functions in conjunction with SCF (Skp1.Cullin 1.F-box) E3 ubiquitin ligase to catalyze covalent attachment of polyubiquitin chains to a target protein...
  93. doi Modeling sporadic Parkinson's disease by silencing the ubiquitin E3 ligase component, SKP1A
    Silvia A Mandel
    Eve Topf Center for Neurodegenerative Diseases Research and Department of Molecular Pharmacology, Faculty of Medicine Technion, Haifa, Israel
    Parkinsonism Relat Disord 15:S148-51. 2009
    ..One of them, SKP1A (p19, S-phase kinase-associated protein 1A), an essential component of the ubiquitin-E3 ligase Skp1, Cullin 1, F-..
  94. ncbi Skp1 and the F-box protein Pof6 are essential for cell separation in fission yeast
    Damien Hermand
    Laboratoire de Genetique Moleculaire GEMO, University of Namur Facultés Universitaires Notre Dame de la Paix, 61 rue de Bruxelles, 5000 Namur, Belgium
    J Biol Chem 278:9671-7. 2003
    Here we report functional characterization of the essential fission yeast Skp1 homologue. We have created a conditional allele of skp1 (skp1-3f) mimicking the mutation in the budding yeast skp1-3 allele...
  95. ncbi Ubiquitination of p21Cip1/WAF1 by SCFSkp2: substrate requirement and ubiquitination site selection
    Wei Wang
    Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA
    Biochemistry 44:14553-64. 2005
    ..The SCF(Skp2) complex (consisting of Rbx1, Cul1, Skp1, and Skp2) is one of the E3 ubiquitin ligases involved in ubiquitination of p21(Cip1/WAF1)...
  96. ncbi The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an F-box protein required for normal patterning and growth in the floral meristem
    A Samach
    Department of Botany, University of British Columbia, Vancouver, BC V6T1Z4, Canada
    Plant J 20:433-45. 1999
    ..mode of action of the UFO gene product, we show here that UFO is an F-box protein that interacts with Arabidopsis SKP1-like proteins, both in the yeast two-hybrid system and in vitro...
  97. ncbi The SCF ubiquitin ligase protein slimb regulates centrosome duplication in Drosophila
    E J Wojcik
    Department of Biology, Virginia Tech University, Blacksburg, Virginia, 24061, USA
    Curr Biol 10:1131-4. 2000
    ..The stability of cyclin E is regulated by the ubiquitin ligase SCF, which is a protein complex composed of Skp1, Cdc53 (Cullin) and F-box proteins [10-12]...
  98. ncbi A new function of Skp1 in the mitotic exit of budding yeast Saccharomyces cerevisiae
    Namil Kim
    Department of Biochemistry and Institute of Life Science and Biotechnology, College of Science, Yonsei University, Seoul 120 749, Republic of Korea
    J Microbiol 44:641-8. 2006
    We previously reported that Skp1, a component of the Skp1-Cullin-F-box protein (SCF) complex essential for the timely degradation of cell cycle proteins by ubiquitination, physically interacts with Bfa1, which is a key negative regulator ..
  99. ncbi Selective cochlear degeneration in mice lacking the F-box protein, Fbx2, a glycoprotein-specific ubiquitin ligase subunit
    Rick F Nelson
    Medical Scientist Training Program, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Neurosci 27:5163-71. 2007
    ..Cochlear Fbx2 binds Skp1, the common binding partner for F-box proteins, and is an unusually abundant inner ear protein...
  100. pmc Activation of the S-phase checkpoint inhibits degradation of the F-box protein Dia2
    Andrew C Kile
    Department of Genetics, Cell Biology and Development, 6 160 Jackson Hall, 321 Church St, S E, Minneapolis, MN 55455, USA
    Mol Cell Biol 30:160-71. 2010
    ..SCF (Skp1/Cul1/F-box protein) complexes are modular ubiquitin ligases...
  101. pmc The role of two f-box proteins, SLEEPY1 and SNEEZY, in Arabidopsis gibberellin signaling
    Tohru Ariizumi
    United States Department of Agriculture Agricultural Research Service, Wheat Genetics, Quality Physiology and Disease Research Unit, Pullman, Washington 99164 6420, USA
    Plant Physiol 155:765-75. 2011
    ..GA responses are repressed by DELLA family proteins. GA relieves DELLA repression when the SCF(SLY1) (for Skp1, Cullin, F-box) E3 ubiquitin ligase ubiquitinates DELLA protein, thereby targeting it for proteolysis...

Research Grants50

  1. Yi Sun; Fiscal Year: 2016
    ..To this end, we have focused on SCF (Skp1-Cullin-F-box proteins) E3 ubiquitin ligases, also known as CRLs (Cullin-RING ubiquitin ligases)...
  2. B-RAF Regulation of the Cell Cycle in Melanoma
    Andrew E Aplin; Fiscal Year: 2013
    ..that mutant B-RAF signaling and cyclin D1 regulate p27Kip1 via expression of Skp2 F- box protein, a component of a Skp1/Cullin/F-box (SCF) ubiquitin ligase complex that mediates p27KiP1 degradation...
  3. Yi Sun; Fiscal Year: 2016
    ..this end, we have focused on SAG (Sensitive to Apoptosis Gene), also known as RBX2/ROC2, a RING component of SCF (Skp1-Cullin-F-box proteins) E3 ubiquitin ligases, required for its activity...
  4. Yi Sun; Fiscal Year: 2014
    ..Sensitive to Apoptosis Gene (SAG, human;Sag, mouse) also known as RBX2/ROC2, is an essential RING component of SCF (SKP1, Cullins, F-box proteins) E3 ubiquitin ligase which, by promoting ubiquitination and degradation of various key ..
  5. Regulation of Cellular Growth and Division by Ubiquitin-Mediated Proteolysis
    Jennifer A Benanti; Fiscal Year: 2012
    ..ubiquitin ligase complexes that are known to regulate the cell cycle are the Anaphase Promoting Complex (APC) and Skp1-Cul1-F-box (SCF) complex...
  6. Yi Sun; Fiscal Year: 2015
    ..SAG (Sensitive to Apoptosis Gene), also known as RBX2/ROC2, is a stress-responsive RING component of SCF (Skp1, Cullins, F-box proteins) E3 ubiquitin ligase, required for its activity...
  7. Role of the SCF/FBX011 ubiquitin ligase in the control of cell proliferation
    Michele Pagano; Fiscal Year: 2013
    ..SCF (Skp1, Cul1, F-box protein) complexes (also known as Cul1-Ring-ligases or CRL1) represent a family of E3 ubiquitin ..
  8. Role of the SCF/Fbxo15 ubiquitin ligase in stem cells and cancer biology
    Michele Pagano; Fiscal Year: 2012
    ..In humans, there are 69 SCF ligases, each characterized by invariable, structural elements (Skp1, Cul1, and Rbx1) and a variable F- box protein subunit that provides specificity by directly recruiting the ..
  9. Jason A Carlyon; Fiscal Year: 2014
    ..We refer to these as Ank/F-box proteins. Eukaryotic F-boxes bind SKP1, a part of the SCF1 ubiquitin ligase complex, which catalyzes the transfer of ubiquitin onto proteins to target ..
  10. MECHANISMS OF CELL CYCLE CONTROL DURING MAMMARY GLAND DE
    Hui Zhang; Fiscal Year: 2001
    ..studies using mouse as an animal model system to determine the roles of recently identified SCF SKP2 complex (Skp1, Cullins/CDC53, F-box proteins), an ubiquitin E3 ligase, during the G1/S transition and the tumorigenic potential ..
  11. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth M Wilson; Fiscal Year: 2010
    ..Skp2 (S phase kinase-associated protein 2) to test our hypothesis that MAGE-11 functions as a subunit of the SCF (Skp1-cullin-Skp2 F-box) E3 ubiquitin ligase complex...
  12. R Suzanne Zukin; Fiscal Year: 2016
    ..fundamental mechanism by which REST abundance is regulated in pluripotent cells and neural progenitors is via SCF (Skp1-Cul1-F-box protein)/?-TrCP-dependent, ubiquitin-based proteasomal degradation...
  13. BRITNEY LANE MOSS; Fiscal Year: 2015
    ..The SKP1-Cullin-F-box (SCF) class of E3s uses F-box proteins as modular substrate-recognition subunits and is highly ..
  14. Christopher M West; Fiscal Year: 2016
    ..A major group of polyubiquitin ligases, the SCF (Skp1-cullin1-Fbox) subclass of cullin-RING-type E3 ubiquitin (Ub)-ligases (CRLs), has been directly implicated in ..
  15. Bing Hao; Fiscal Year: 2016
    ..We will a) characterize in detail the structural properties of the Skp1-Fbx4-TRF1 complex and identify specific amino-acid side chains involved in the E3-substrate interaction;b) generate ..
  16. Regulation of Cellular Growth and Division by Ubiquitin-Mediated Proteolysis
    JENNIFER BENANTI; Fiscal Year: 2009
    ..ubiquitin ligase complexes that are known to regulate the cell cycle are the Anaphase Promoting Complex (ARC) and Skp1-Cul1-F-box (SCF) complex...
  17. Michele Pagano; Fiscal Year: 2016
    DESCRIPTION (provided by applicant): Mammalian SCF (Skp1/Cul1/F-box protein) ubiquitin ligases are critical for the activation and attenuation of many cellular processes...
  18. Interaction Between SV40 Large T and p185 Cullin
    James A DeCaprio; Fiscal Year: 2011
    ..CUL7 binds specifically to FBXW8 to form an SCF-like (SKP1-Cullin-F box) E3 ubiquitin ligase...
  19. Coordinated regulation of MAP kinase signaling by ubiquitination
    JILLIAN HEYWARD HURST; Fiscal Year: 2012
    ..Mutations of Skp1/Cullin/F-box E3 ubiquitin ligase complexes (SCF) have been linked to many human diseases, including multiple forms ..
  20. Christopher M West; Fiscal Year: 2016
    ..The initial organism of analysis is the social amoeba Dictyostelium, and the target of the pathway studied here is Skp1, an adaptor of the SCF class of E3 ubiquitin ligases whose targets are frequently activated by phosphorylation and ..
  21. Structural biology of SCFs and related ubiquitin Ligases
    Nikola Pavletich; Fiscal Year: 2005
    ..The SCF E3 consists of an F-box protein, Skp1, Cull and Rbxl, and is by far the largest E3 family known to date, with hundreds of SCF subunits in the databases...
  22. F box Proteins and Deregulated Proteolysis in Cancer
    Michele Pagano; Fiscal Year: 2005
    ..cellular regulators is controlled by ubiquitin ligases called SCFs since they are composed of three major subunits: Skp1, cull and one of many E-box proteins (Fbps)...
  23. ENVIRONMENTAL MODULATION OF FIBRINOLYSIS INHIBITORS
    STEVEN GONIAS; Fiscal Year: 1992
    ..these studies will be extended to consider thr regulation of plasminogen activators such as streptokinase- plasmin (SkP1) and tissue plasminogen activator in the same environments...
  24. 800 MHz Spectrometer for Biomolecular NMR in Missouri
    STEVEN VAN DOREN; Fiscal Year: 2006
    ..Henzl will study structural features and interactions of principal proteins (OCP2 and OCP1) in the auditory organ of the inner ear, a complex that binds connexin 26 most often mutated in hereditary ..
  25. SCF Complexes: Key to Understanding Cochlear Homeostasis
    Isolde Thalmann; Fiscal Year: 2006
    ..cell types of the ESC - an elaborate epithelial gap-junction system (EGJS) and high-level expression of OCP1 and OCP2. Formed by hexagonal arrays of connexin molecules, gap-junctions facilitate movement of low molecular weight ..
  26. A Skpl-containing Complex Regulating V-ATPase Activity
    Patricia Kane; Fiscal Year: 2005
    ..b>Skp1 is a highly conserved protein that plays a central role in ubiquitin-dependent proteolysis as an essential member ..
  27. YEAST G1 CYCLIN DEGRADATION BY THE SCF UBIQUITIN LIGASE
    Robb Moses; Fiscal Year: 2003
    ..A highly conserved protein degradation complex termed SCF (for Skp1-Cdc53-F-box protein) is thought to recognize and mark Gl cyclins (as well as a number of other regulatory proteins) ..
  28. Ubiquitination and Degradation in Cancer Therapy
    Kathleen Sakamoto; Fiscal Year: 2005
    ..A protein complex known as, the SCF (Skp1, Cullin, F-box, and Hrtl/Rbx) ubiquitin ligase specifically targets proteins for ubiquitination and subsequent ..
  29. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Gabriel Pineda; Fiscal Year: 2006
    ..BTrCP is an F-Box protein that also contains a WD40 repeat motif. The F-box region of the protein binds Skp1, Cul1, and mediates the binding of an E2 (Ubch5c). The WD40 repeat motif binds phophorylated IkBa...
  30. Substrates of SAG-SCF E3 ligase regulate hypoxia response
    Yi Sun; Fiscal Year: 2007
    ..These initial studies will set the stage for a more detailed investigation of the mechanism of SAG mediated suppression of apoptosis. [unreadable] [unreadable]..
  31. SAG E3 ubiquitin ligase in angiogenesis & carcinogenesis
    Yi Sun; Fiscal Year: 2010
    ..Apoptosis Gone), also known as RBX2IROC2, or RNF7 (RING finger protein 7), is a stress-responsive component of SCF (Skp1, Cullins, F-box proteins) E3 ubiquitin ligase...
  32. Molecular and Cellular Characterization of Myeloproliferative Disease
    KATHLEEN MIHO SAKAMOTO; Fiscal Year: 2010
    ..These studies will provide new insights into the molecular pathways leading to MPD. ..
  33. Air Pollution and Adverse Birth Outcomes
    Beate Ritz; Fiscal Year: 2004
    ..abstract_text> ..
  34. PPK: Radiation Response and Cell Proliferation
    Peter Stambrook; Fiscal Year: 2006
    ..abstract_text> ..
  35. Ergonomic Interventions for Garment Work
    Beate Ritz; Fiscal Year: 2004
    ..This study will provide employers, employees and public agencies with evidence of the effectiveness of ergonomic interventions in order to guide health and safety policy. ..
  36. Environmental Mutagen Society 38th Annual Meeting
    Peter Stambrook; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  37. Student and Postdoctoral Level Investigator Travel Awards at 2005 EMS Meeting
    Peter Stambrook; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  38. Pathways to Mutagenesis in vivo and in Stem Cells
    Peter Stambrook; Fiscal Year: 2009
    ..This experiment should provide evidence for or against the "top down" model of colon cancer that has been proposed. ..
  39. Registry Study of Parkinsons Disease in Denmark
    Beate R Ritz; Fiscal Year: 2010
    ..The proposed research cannot be performed in the US due to a lack of population PD registries that would provide access to such a large number of cases and valid methods for control recruitment from the general population. ..
  40. THE S. CEREVISIAE S PHASE CHECKPOINT
    Steven Reed; Fiscal Year: 2003
    ....
  41. Receptor-Mediated Endocytosis of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2004
    ..Our studies may uncover potential pathogenic evolution of Lpn to invade the more evolved mammalian cells, and may contribute to the understanding of invasion of protozoa by Mycobacterium and Chlamydia. ..
  42. MOLECULAR AND GENETIC BASIS OF CELL PROLIFERATION
    MICHAEL TYERS; Fiscal Year: 2005
    ..The Gordon Conferences format provides a unique environment that encourages informal and open discussion among the participants and this in turn fosters initiation of collaborative efforts and stimulates future research directions. ..
  43. Using Synthetic Lethality to Select Cancer Drug Targets
    William Kaelin; Fiscal Year: 2005
    ..Moreover, they may establish a new paradigm for the selection of anticancer drug targets based on inactivating mutations of TSG. ..
  44. Genistein induces thymic atrophy: a health concern?
    Paul Cooke; Fiscal Year: 2006
    ..These studies will advance our understanding of isoflavone effects on the immune system, and also may have relevance for soy-fed infants and laboratory rodents consuming soy-based feed. ..
  45. The Pore-forming Toxin of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2003
    ..Our studies on L. pneumophila may provide a paradigm for other vacuolar intracellular pathogens such as Mycobacterium, Salmonella, and Chlamydia. ..
  46. Real-Time Imaging of Hypoxia based on VHL Activity
    William Kaelin; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  47. 2006 Cancer Model Mechanisms
    William Kaelin; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  48. Molecular pathogenesis of Francisella tularnesis
    YOUSEF A ABU KWAIK; Fiscal Year: 2010
    ..The bacterial effectors involved in exploiting the macrophage are potential targets for treatment as well as potential vaccine candidates. ..
  49. The role of Cyclin E in growth control and tumorogenesis
    Steven Reed; Fiscal Year: 2008
    ..It is hoped that insights gained from understanding these fundamental mechanisms of oncogenesis will lead to new approaches to cancer prevention and therapy. ..
  50. A Luciferase Fusion Protein Library to Identify & Monitor Ubiquitylation Targets
    William Kaelin; Fiscal Year: 2008
    ..Reisolation of HIF in aims 2 and 3 would constitute proof of concept with respect to the potential utility of this approach. ..