SIRT5

Summary

Gene Symbol: SIRT5
Description: sirtuin 5
Alias: SIR2L5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, NAD-dependent deacetylase sirtuin-5, NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial, silent mating type information regulation 2, S.cerevisiae, homolog 5, sir2-like 5, sirtuin type 5
Species: human

Top Publications

  1. ncbi Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins
    R A Frye
    Pittsburgh V A Medical Center 132L, University, Pittsburgh, Pennsylvania 15240, USA
    Biochem Biophys Res Commun 273:793-8. 2000
  2. ncbi Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization
    U Mahlknecht
    Department of Hematology Oncology, University of Heidelberg Medical Center, Heidelberg, Germany
    Cytogenet Genome Res 112:208-12. 2006
  3. ncbi Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase
    Nidhi Ahuja
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94158, USA
    J Biol Chem 282:33583-92. 2007
  4. doi Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms
    Nobuko Matsushita
    Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Genes Cells 16:190-202. 2011
  5. pmc The first identification of lysine malonylation substrates and its regulatory enzyme
    Chao Peng
    Ben May Department for Cancer Research, The University of Chicago, Chicago, IL 60637, USA
    Mol Cell Proteomics 10:M111.012658. 2011
  6. pmc Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase
    Jintang Du
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Science 334:806-9. 2011
  7. doi Altered expression of SIRT gene family in head and neck squamous cell carcinoma
    Chi Chih Lai
    Division of Laryngology, Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 123 Da Pi Rd, Niaosong District, 833 Kaohsiung City, Taiwan
    Tumour Biol 34:1847-54. 2013
  8. pmc SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways
    Jeongsoon Park
    Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 50:919-30. 2013
  9. doi SIRT5 desuccinylates and activates SOD1 to eliminate ROS
    Zhi Feng Lin
    Department of Thoracic Surgery, Shanghai First People s Hospital, Shanghai Jiaotong University, Shanghai 200080, China
    Biochem Biophys Res Commun 441:191-5. 2013
  10. pmc Lysine glutarylation is a protein posttranslational modification regulated by SIRT5
    Minjia Tan
    The Chemical Proteomics Center and State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People s Republic of China
    Cell Metab 19:605-17. 2014

Research Grants

  1. Eric S Goetzman; Fiscal Year: 2016
  2. High-throughput assays for the development of SIRT5-specific inhibitors
    Hening Lin; Fiscal Year: 2012
  3. DAVID BENNER LOMBARD; Fiscal Year: 2016
  4. Chemical Tools to Visualize Sirtuin Activity in Cells
    Anthony A Sauve; Fiscal Year: 2013
  5. Kelly E Sullivan; Fiscal Year: 2015
  6. Etienne L Sibille; Fiscal Year: 2015
  7. Robert S Weiss; Fiscal Year: 2015
  8. Robert T Kennedy; Fiscal Year: 2015
  9. Synergistic Nutraceutical Effects of DLPC and SAMe
    Charles Lieber; Fiscal Year: 2007
  10. ROLE OF CHROMATIN IN HIV TRANSCRIPTIONAL REGULATION
    ERIC M VERDIN; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications101 found, 100 shown here

  1. ncbi Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins
    R A Frye
    Pittsburgh V A Medical Center 132L, University, Pittsburgh, Pennsylvania 15240, USA
    Biochem Biophys Res Commun 273:793-8. 2000
    ..The seven human sirtuin genes include all four classes: SIRT1, SIRT2, and SIRT3 are class I, SIRT4 is class II, SIRT5 is class III, and SIRT6 and SIRT7 are class IV.
  2. ncbi Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization
    U Mahlknecht
    Department of Hematology Oncology, University of Heidelberg Medical Center, Heidelberg, Germany
    Cytogenet Genome Res 112:208-12. 2006
    Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase and belongs to the Silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (HDACs), which play a central role in epigenetic gene ..
  3. ncbi Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase
    Nidhi Ahuja
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94158, USA
    J Biol Chem 282:33583-92. 2007
    ..Depletion of SIRT4 from insulin-producing INS-1E cells results in increased insulin secretion in response to glucose. These observations define a new role for mitochondrial SIRT4 in the regulation of insulin secretion...
  4. doi Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms
    Nobuko Matsushita
    Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Genes Cells 16:190-202. 2011
    ..In this study, we clarified the mechanism of subcellular localization of two SIRT5 isoforms (i.e...
  5. pmc The first identification of lysine malonylation substrates and its regulatory enzyme
    Chao Peng
    Ben May Department for Cancer Research, The University of Chicago, Chicago, IL 60637, USA
    Mol Cell Proteomics 10:M111.012658. 2011
    ..In addition, we demonstrate that Sirt5, a member of the class III lysine deacetylases, can catalyze lysine demalonylation and lysine desuccinylation ..
  6. pmc Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase
    Jintang Du
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Science 334:806-9. 2011
    ..Four of them (Sirt4 to Sirt7) have no detectable or very weak deacetylase activity. We found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro...
  7. doi Altered expression of SIRT gene family in head and neck squamous cell carcinoma
    Chi Chih Lai
    Division of Laryngology, Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 123 Da Pi Rd, Niaosong District, 833 Kaohsiung City, Taiwan
    Tumour Biol 34:1847-54. 2013
    ..Our results demonstrated that the expression levels of SIRT1, SIRT2, SIRT3, SIRT5, SIRT6, and SIRT7 were significantly downregulated in cancerous tissues compared with noncancerous tissues (all p&..
  8. pmc SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways
    Jeongsoon Park
    Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 50:919-30. 2013
    Protein function is regulated by diverse posttranslational modifications. The mitochondrial sirtuin SIRT5 removes malonyl and succinyl moieties from target lysines...
  9. doi SIRT5 desuccinylates and activates SOD1 to eliminate ROS
    Zhi Feng Lin
    Department of Thoracic Surgery, Shanghai First People s Hospital, Shanghai Jiaotong University, Shanghai 200080, China
    Biochem Biophys Res Commun 441:191-5. 2013
    ..Here, we report that SOD1 is succinylated and that succinylation decreases its activity. SIRT5 binds to, desuccinylates and activates SOD1. SOD1-mediated ROS reduction is increased when SIRT5 is co-expressed...
  10. pmc Lysine glutarylation is a protein posttranslational modification regulated by SIRT5
    Minjia Tan
    The Chemical Proteomics Center and State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People s Republic of China
    Cell Metab 19:605-17. 2014
    ..We demonstrated that the previously annotated deacetylase, sirtuin 5 (SIRT5), is a lysine deglutarylase...
  11. doi Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5
    Christine Schlicker
    Laboratory of Biochemistry, Department of Physiological Chemistry, Ruhr University Bochum, Universitaetsstrasse 150, 44801 Bochum, Germany
    J Mol Biol 382:790-801. 2008
    ..Mammalian mitochondria contain three Sirtuins, Sirt3, Sirt4, and Sirt5. Only one substrate is known for Sirt3 as well as for Sirt4, and up to now, no target for Sirt5 has been reported...
  12. doi SIRT5 is under the control of PGC-1α and AMPK and is involved in regulation of mitochondrial energy metabolism
    Marcin Buler
    Department of Pharmacology and Toxicology, Institute of Biomedicine, Medical Research Center Oulu and
    FASEB J 28:3225-37. 2014
    ..Overexpression of PGC-1α in mouse primary hepatocytes increased SIRT5 mRNA expression 4-fold and also the protein in a peroxisome proliferator-activated receptor α (PPARα)- and ..
  13. doi A succinyl lysine-based photo-cross-linking peptide probe for Sirtuin 5
    Karunakaran A Kalesh
    Department of Chemistry, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK
    Org Biomol Chem 12:4310-3. 2014
    ..The probe, not only displayed robust labelling performance with purified Sirt5, but also enabled sensitive detection of the hydrolase in the presence of large excess of cellular proteins...
  14. pmc Deacetylation of the tumor suppressor protein PML regulates hydrogen peroxide-induced cell death
    D Guan
    Department of Biochemistry, School of Medicine, Case Western Reserve University, and Comprehensive Cancer Center of Case Western Reserve University, Cleveland, OH, USA
    Cell Death Dis 5:e1340. 2014
    ..We found that hydrogen peroxide (H2O2) promotes PML deacetylation and identified SIRT1 and SIRT5 as PML deacetylases...
  15. doi Chemical probing of the human sirtuin 5 active site reveals its substrate acyl specificity and peptide-based inhibitors
    Claudia Roessler
    Department of Enzymology, Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle Saale Germany
    Angew Chem Int Ed Engl 53:10728-32. 2014
    ..derived peptide substrate and modified the lysine side chain systematically to determine the acyl specificity of Sirt5. From that point we designed six potent peptide-based inhibitors that interact with the NAD(+) binding pocket...
  16. pmc Overexpression of mitochondrial sirtuins alters glycolysis and mitochondrial function in HEK293 cells
    Michelle Barbi de Moura
    Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 9:e106028. 2014
    SIRT3, SIRT4, and SIRT5 are mitochondrial deacylases that impact multiple facets of energy metabolism and mitochondrial function...
  17. ncbi Direct evidence of sirtuin downregulation in the liver of non-alcoholic fatty liver disease patients
    Tao Wu
    Department of Lemology, The First Affiliated Hospital of Shantou University Medical College, Shantou Laboratory of Cell Senescence, Shantou University Medical College, Shantou
    Ann Clin Lab Sci 44:410-8. 2014
    ..For the purposes of this study, we hypothesized that the inhibition of sirtuin signaling might contribute to the development of NAFLD...
  18. pmc Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity
    Rommel A Mathias
    Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, 3086, Australia
    Cell 159:1615-25. 2014
    ..Despite conserved deacetylase domains, mitochondrial SIRT4 and SIRT5 have little to no deacetylase activity, and a robust catalytic activity for SIRT4 has been elusive...
  19. pmc Mitochondrial sirtuins and their relationships with metabolic disease and cancer
    Surinder Kumar
    1 Department of Pathology, University of Michigan, Ann Arbor, Michigan
    Antioxid Redox Signal 22:1060-77. 2015
    ..Mitochondrial sirtuins (SIRT3, SIRT4, and SIRT5) play pivotal roles in promoting this homeostasis by regulating numerous aspects of mitochondrial metabolism in ..
  20. pmc SIRT5 regulation of ammonia-induced autophagy and mitophagy
    Lucia Polletta
    a Department of Experimental Medicine University of Rome Sapienza Rome, Italy
    Autophagy 11:253-70. 2015
    In liver the mitochondrial sirtuin, SIRT5, controls ammonia detoxification by regulating CPS1, the first enzyme of the urea cycle...
  21. pmc SIRT3 and SIRT5 regulate the enzyme activity and cardiolipin binding of very long-chain acyl-CoA dehydrogenase
    Yuxun Zhang
    Department of Pediatrics, University of Pittsburgh, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 10:e0122297. 2015
    SIRT3 and SIRT5 have been shown to regulate mitochondrial fatty acid oxidation but the molecular mechanisms behind the regulation are lacking...
  22. pmc The role of sirtuins in cardiac disease
    Shouji Matsushima
    Department of Cell Biology and Molecular Medicine, Cardiovascular Research Institute, Rutgers New Jersey Medical School, Newark, New Jersey and Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Am J Physiol Heart Circ Physiol 309:H1375-89. 2015
    ..On the other hand, the roles of Sirt4 and Sirt5 in the heart remain largely uncharacterized.
  23. doi Characterization of the SIRT family of NAD+-dependent protein deacetylases in the context of a mammalian model of hibernation, the thirteen-lined ground squirrel
    Andrew N Rouble
    Institute of Biochemistry and Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada
    Cryobiology 71:334-43. 2015
    ..To assess this possibility, protein levels of the seven mammalian SIRTs (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT7), total SIRT activity, and the acetylation status of two downstream SIRT targets (SOD2K68 and NF-..
  24. doi A Novel Continuous Assay for the Deacylase Sirtuin 5 and Other Deacetylases
    Claudia Roessler
    Department of Enzymology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle Saale, Germany
    J Med Chem 58:7217-23. 2015
    ..Systematic optimization of a carbamoyl phosphate synthetase 1 derived, glutarylated peptide yielded a Sirt5 substrate with k(cat)/K(M) value of 337,000 M(-1) s(-1), which represents the best sirtuin substrate described so ..
  25. pmc Proteomic and Biochemical Studies of Lysine Malonylation Suggest Its Malonic Aciduria-associated Regulatory Role in Mitochondrial Function and Fatty Acid Oxidation
    Gozde Colak
    From the Ben May Department of Cancer Research, University of Chicago, Chicago, Illinois 60637
    Mol Cell Proteomics 14:3056-71. 2015
    ..Our study establishes an association between Kmal and a genetic disease and offers a rich resource for elucidating the contribution of the Kmal pathway and malonyl-CoA to cellular physiology and human diseases. ..
  26. pmc Characterization of the cardiac succinylome and its role in ischemia-reperfusion injury
    Jennifer A Boylston
    Systems Biology Center, NHLBI, NIH, Bethesda, MD, United States
    J Mol Cell Cardiol 88:73-81. 2015
    Succinylation refers to modification of lysine residues with succinyl groups donated by succinyl-CoA. Sirtuin5 (Sirt5) is a mitochondrial NAD(+)-dependent deacylase that catalyzes the removal of succinyl groups from proteins...
  27. pmc Aging and chronic administration of serotonin-selective reuptake inhibitor citalopram upregulate Sirt4 gene expression in the preoptic area of male mice
    Dutt Way Wong
    Brain Research Institute, School of Medicine and Health Sciences, Monash University Malaysia Selangor, Malaysia
    Front Genet 6:281. 2015
    ..Age-related increases of sirt1, sirt4, sirt5, and sirt7 mRNA levels were observed in the POA of 52 weeks old mice...
  28. doi The expression levels of the sirtuins in patients with BCC
    Metin Temel
    Department of Plastic and Reconstructive Surgery, School of Medicine, Mustafa Kemal University, Hatay, Turkey
    Tumour Biol 37:6429-35. 2016
    ..1, Sirt1.2, Sirt2, Sirt3, Sirt4, Sirt5, Sirt6, and Sirt7) were examined by real-time PCR. The results showed that expressions of SIRT1.1, SIRT1...
  29. pmc Deposition of 5-Methylcytosine on Enhancer RNAs Enables the Coactivator Function of PGC-1α
    Francesca Aguilo
    Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA Department of Structural and Chemical Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
    Cell Rep 14:479-92. 2016
    ..loss of Set7/9 and NSun7 in liver cell model systems resulted in depletion of the PGC-1α target genes Pfkl, Sirt5, Idh3b, and Hmox2, which was accompanied by a decrease in the eRNAs levels associated with these loci...
  30. pmc Sirtuins, Cell Senescence, and Vascular Aging
    Yujiro Kida
    Departments of Medicine, Pharmacology, and Physiology, Renal Research Institute, New York Medical College, Valhalla, New York, USA Electronic address
    Can J Cardiol 32:634-41. 2016
    ..SIRT1 and SIRT2 are localized in the nucleus and cytoplasm. SIRT3, SIRT4, and SIRT5 are mitochondrial, and SIRT6 and SIRT7 are nuclear...
  31. pmc Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme
    Junru Yu
    Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota, United States
    PLoS ONE 11:e0152467. 2016
    ..similarity among all seven isoforms of sirtuins (of which most of them catalyze the deacetylation reaction), SIRT5 is the only isoform that catalyzes the cleavage of negatively charged acylated substrates, and the latter feature ..
  32. pmc SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense
    Lisha Zhou
    Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Fudan University, Shanghai, China Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China Molecular and Cell Biology Lab, Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
    EMBO Rep 17:811-22. 2016
    ..Here, we report that SIRT5 desuccinylates and deglutarylates isocitrate dehydrogenase 2 (IDH2) and glucose-6-phosphate dehydrogenase (G6PD), ..
  33. pmc Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators
    Rommel A Mathias
    Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Room 423 LIMS1, Melbourne, Vic, 3086, Australia
    Methods Mol Biol 1436:213-39. 2016
    Recent studies have highlighted the three mitochondrial human sirtuins (SIRT3, SIRT4, and SIRT5) as critical regulators of a wide range of cellular metabolic pathways...
  34. pmc Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein
    Surinder Kumar
    Department of Pathology, University of Michigan, Ann Arbor, MI, 48109, USA
    Methods Mol Biol 1436:241-57. 2016
    Sirtuin-family deacylases promote health and longevity in mammals. The sirtuin SIRT5 localizes predominantly to the mitochondrial matrix...
  35. pmc SIRT5 Deficiency Enhances Susceptibility to Kainate-Induced Seizures and Exacerbates Hippocampal Neurodegeneration not through Mitochondrial Antioxidant Enzyme SOD2
    Fengling Li
    Department of Pharmacy, Linyi Tumor Hospital Linyi, Shandong, China
    Front Cell Neurosci 10:171. 2016
    ..Sirtuin 5 (SIRT5), localized in mitochondrial matrix, has been considered as an important functional modulator of mitochondria that ..
  36. pmc A Novel Role for SIRT3 in Regulating Mediators Involved in the Terminal Pathways of Human Labor and Delivery
    Ratana Lim
    Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia
    Biol Reprod 95:95. 2016
    ..In this study, we determined the effect of term labor and pro-inflammatory cytokines on SIRT3, SIRT4, SIRT5, and SIRT7 expression in human myometrium...
  37. pmc NAMPT-Mediated NAD(+) Biosynthesis Is Essential for Vision In Mice
    Jonathan B Lin
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA Neuroscience Graduate Program, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Rep 17:69-85. 2016
    ..We further demonstrate that the NAD(+)-dependent mitochondrial deacylases SIRT3 and SIRT5 play important roles in retinal homeostasis and that NAD(+) deficiency causes SIRT3 dysfunction...
  38. pmc In low protein diets, microRNA-19b regulates urea synthesis by targeting SIRT5
    Rui Ping Sun
    College of Animal Science, Chinese National Centre of Pig Breeding Technology, ALLTECH SCAU Animal Nutrition Control Research Alliance, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, 483 Wushan Road, Guangzhou, 510642, China
    Sci Rep 6:33291. 2016
    ..MiR-19b negatively regulated urea synthesis by targeting SIRT5, which is a positive regulator of CPS1, the rate limiting enzyme in the urea cycle...
  39. pmc Segmental Aging Underlies the Development of a Parkinson Phenotype in the AS/AGU Rat
    Sohair M Khojah
    School of Life Sciences, Pharmacology Research Theme, University of Glasgow, Glasgow G12 8QQ, UK
    Cells 5:. 2016
    ..We have used biomarkers of aging (SA β-Gal, p16Ink4a, Sirt5, Sirt6, and Sirt7) to demonstrate the presence of an accelerated aging phenotype across different brain regions in ..
  40. ncbi Sirtuin 5: a review of structure, known inhibitors and clues for developing new inhibitors
    LingLing Yang
    College of Food and Bioengineering, Xihua University, Sichuan, 610039, China
    Sci China Life Sci . 2016
    ..Very recently, sirtuin 5 (SIRT5) has received considerable attention due to that it was found to have weak deacetylase activity but strong ..
  41. doi Alpha-synuclein alters differently gene expression of Sirts, PARPs and other stress response proteins: implications for neurodegenerative disorders
    J Motyl
    Department of Cellular Signalling, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, Warsaw, Poland
    Mol Neurobiol . 2017
    ..Moreover, eASN upregulated expression of Sirt3 and Sirt5, but downregulated of Sirt1, which plays an important role in cell metabolism including Aβ precursor protein (APP)..
  42. ncbi Differential regulation of the Sir2 histone deacetylase gene family by inhibitors of class I and II histone deacetylases
    S Kyrylenko
    Department of Neuroscience and Neurology, University of Kuopio, 70211 Kuopio, Finland
    Cell Mol Life Sci 60:1990-7. 2003
    ..SIRT2, SIRT4 and SIRT7 were upregulated, whereas SIRT1, SIRT5 and SIRT6 were downregulated by trichostatin A (TSA) and n-butyrate...
  43. doi Structural insights of SIR2rp3 proteins as promising biotargets to fight against Chagas disease and leishmaniasis
    Lionel Sacconnay
    Pharmacochemistry and Phytochemistry and Bioactive Natural Products, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet 30, CH 1211 Geneva 4, Switzerland
    Mol Biosyst 9:2223-30. 2013
    ..Molecular docking of known inhibitors revealed strong analogies with the mitochondrial human SIRT5 in terms of binding mode and interaction strength...
  44. pmc Widespread and enzyme-independent Nε-acetylation and Nε-succinylation of proteins in the chemical conditions of the mitochondrial matrix
    Gregory R Wagner
    From the Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana 46202 and
    J Biol Chem 288:29036-45. 2013
    ..These findings may have implications for the evolutionary roles that the mitochondria-localized SIRT3 deacetylase and SIRT5 desuccinylase have in the maintenance of metabolic health.
  45. doi An acetylome peptide microarray reveals specificities and deacetylation substrates for all human sirtuin isoforms
    David Rauh
    Department of Enzymology, Institute for Biochemistry and Biotechnology, Martin Luther University, Kurt Mothes Staße 3, 06120 Halle Saale, Germany
    Nat Commun 4:2327. 2013
    ..protein as a Sirt3 substrate and show that peroxiredoxin 1 and high-mobility group B1 protein are deacetylated by Sirt5 and Sirt1, respectively, at the identified sites, rendering them likely new in vivo substrates...
  46. doi Dietary restriction increases protein acetylation in the livers of aged rats
    Akihiro Nakamura
    Department of Pediatric Cardiology and Nephrology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Gerontology 59:542-8. 2013
    ..Furthermore, the effects of DR on sirtuins (e.g. SIRT1, SIRT2, SIRT3, and SIRT5) have also been reported in mammals...
  47. pmc Oxygen flux analysis to understand the biological function of sirtuins
    Dongning Wang
    Sarah W Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, NC, USA
    Methods Mol Biol 1077:241-58. 2013
    ..Of the seven mammalian sirtuins, three localize to the mitochondria: SIRT3, SIRT4, and SIRT5. Mitochondrial sirtuins are crucial regulators of the metabolic network that controls energy homeostasis and ..
  48. pmc Ethanol metabolism modifies hepatic protein acylation in mice
    Kristofer S Fritz
    Department of Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado, United States of America
    PLoS ONE 8:e75868. 2013
    ..The newly discovered desuccinylase activity of the mitochondrial sirtuin SIRT5 suggests that protein succinylation could be an important post-translational modification regulating mitochondrial ..
  49. pmc Metabolic characterization of a Sirt5 deficient mouse model
    Jiujiu Yu
    Laboratory of Integrative and Systems Physiology LISP NCEM, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Sci Rep 3:2806. 2013
    b>Sirt5, localized in the mitochondria, is a member of sirtuin family of NAD⁺-dependent deacetylases. Sirt5 was shown to deacetylate and activate carbamoyl phosphate synthase 1...
  50. doi Effect of breed on the expression of Sirtuins (Sirt1-7) and antioxidant capacity in porcine brain
    Y Ren
    1 Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No 866 Yuhangtang Road, Hangzhou 310058, People s Republic of China
    Animal 7:1994-8. 2013
    ..the mRNA levels of Sirt1, as well as Sirt2 and Sirt4, in Jinhua pigs were greater, but the mRNA levels of Sirt3, Sirt5, Sirt6, and Sirt7 of Jinhua pigs were lower compared with Danish Landrace pigs...
  51. pmc SIRT5 regulates the mitochondrial lysine succinylome and metabolic networks
    Matthew J Rardin
    Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA
    Cell Metab 18:920-33. 2013
    ..approach to characterize the lysine succinylome in liver mitochondria and its regulation by the desuccinylase SIRT5. A total of 1,190 unique sites were identified as succinylated, and 386 sites across 140 proteins representing ..
  52. doi Cloning, chromosomal characterization and FISH mapping of the NAD(+)-dependent histone deacetylase gene sirtuin 5 in the mouse
    Susanne Voelter-Mahlknecht
    Institute of Occupational and Social Medicine and Health Services Research, University of Tuebingen, D 72074 Tuebingen, Germany
    Int J Oncol 43:237-45. 2013
    Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, belonging to the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (sirtuins)...
  53. pmc Resveratrol stimulates cortisol biosynthesis by activating SIRT-dependent deacetylation of P450scc
    Donghui Li
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California 92093 0704, USA
    Endocrinology 153:3258-68. 2012
    ..The effects of resveratrol on P450scc protein levels and acetylation status are dependent on SIRT3 and SIRT5 expression...
  54. pmc Sirtuin activators and inhibitors
    Jose M Villalba
    Departamento de Biologia Celular, Fisiologia e Inmunologia, Campus Universitario de Rabanales, Edificio Severo Ochoa, Universidad de Cordoba, Campus de Excelencia Internacional Agroalimentario, Cordoba, Spain
    Biofactors 38:349-59. 2012
    ..Sirtuin inhibitors with a wide range of core structures have been identified for SIRT1, SIRT2, SIRT3 and SIRT5 (splitomicin, sirtinol, AGK2, cambinol, suramin, tenovin, salermide, among others)...
  55. pmc The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5)
    Yeyun Zhou
    MacCHESS, Cornell High Energy Synchrotron Source, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 287:28307-14. 2012
    ..Their functions have been generally attributed to NAD-dependent deacetylase activity. However, human SIRT5 (sirtuin 5), which has been reported to exhibit little deacetylase activity, was recently identified as an NAD-..
  56. doi Differential sirtuin expression patterns in amyotrophic lateral sclerosis (ALS) postmortem tissue: neuroprotective or neurotoxic properties of sirtuins in ALS?
    Sonja Körner
    Department of Neurology, Hannover Medical School, Hannover, Germany
    Neurodegener Dis 11:141-52. 2013
    ..We assessed the expression levels of sirtuins in human postmortem ALS and control brain and spinal cord...
  57. doi Synthesis of carba-NAD and the structures of its ternary complexes with SIRT3 and SIRT5
    Bruce G Szczepankiewicz
    Sirtris, a GSK Company, 200 Technology Square, Cambridge, Massachusetts 02139, USA
    J Org Chem 77:7319-29. 2012
    ..SIRT3 and SIRT5 are NAD-consuming enzymes that are potential therapeutic targets for the treatment of metabolic diseases and ..
  58. doi Mitochondrial sirtuins: regulators of protein acylation and metabolism
    Wenjuan He
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, CA 94158, USA
    Trends Endocrinol Metab 23:467-76. 2012
    ..SIRT3 deacetylates and regulates the enzymatic activity of many metabolic enzymes in mitochondria, whereas SIRT5 removes two novel post-translational modifications, lysine malonylation and succinylation...
  59. ncbi Transcription levels of sirtuin family in neural stem cells and brain tissues of adult mice
    H F Wang
    Laboratory of Molecular Neurobiology, School of Life Sciences, and Institute of Systems Biology, Shanghai University, Shanghai 200444, China
    Cell Mol Biol (Noisy-le-grand) . 2012
    ..Results indicated that the expression of sirt1, sirt5 and sirt6 was significant downregulated by nicotinamide treatment...
  60. pmc Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition
    Frank Fischer
    Department of Biochemistry and Research Center for Bio Macromolecules, University of Bayreuth, Bayreuth, Germany
    PLoS ONE 7:e45098. 2012
    ..Using this assay, we show that the deacetylation activity of human Sirt5 features an unusual insensitivity to nicotinamide inhibition...
  61. doi SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice
    Yasuhiko Nakamura
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    FEBS Lett 586:4076-81. 2012
    We identified urate oxidase (UOX) as a target of SIRT5 by comparing mitochondrial proteins in livers of SIRT5-overexpressing transgenic (SIRT5 Tg) and wild-type mice by using two-dimensional electrophoresis...
  62. pmc Mitochondrial protein acylation and intermediary metabolism: regulation by sirtuins and implications for metabolic disease
    John C Newman
    Gladstone Institute of Virology and Immunology, San Francisco, California 94158, USA
    J Biol Chem 287:42436-43. 2012
    ..b>SIRT5 exhibits demalonylase/desuccinylase activity, and lysine succinylation and malonylation are abundant mitochondrial ..
  63. pmc HDAC isoenzyme expression is deregulated in chronic lymphocytic leukemia B-cells and has a complex prognostic significance
    Michael Van Damme
    Laboratory of Clinical Cell Therapy, Faculty of Medicine, Institut Jules Bordet, Universite Libre de Bruxelles ULB, Brussels, Belgium
    Epigenetics 7:1403-12. 2012
    ..03, p < 0.0001). For OS, SIRT5 and 6 allowed stratification into 3 groups, with a median OS of > 360, 237 and 94 mo (HR = 6.38, p < 0.0001)...
  64. pmc Mitochondrial sirtuins and metabolic homeostasis
    Eija Pirinen
    Laboratory for Integrative and Systems Physiology, School of Life Sciences, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Best Pract Res Clin Endocrinol Metab 26:759-70. 2012
    ..The mitochondrial sirtuins SIRT3, SIRT4 and SIRT5 together with the nuclear SIRT1 regulate several aspects of mitochondrial physiology by controlling post-..
  65. pmc A molecular mechanism for direct sirtuin activation by resveratrol
    Melanie Gertz
    Department of Biochemistry, University of Bayreuth, Bayreuth, Germany
    PLoS ONE 7:e49761. 2012
    ..Here we show that resveratrol inhibits human Sirt3 and stimulates Sirt5, in addition to Sirt1, against fluorophore-labeled peptide substrates but also against peptides and proteins ..
  66. doi Inhibition of the human deacylase Sirtuin 5 by the indole GW5074
    Benjamin Suenkel
    Department of Biochemistry, University of Bayreuth, Universitaetsstrasse 30, 95447 Bayreuth, Germany
    Bioorg Med Chem Lett 23:143-6. 2013
    ..Few pharmacological inhibitors have been reported yet for human Sirt5 since substrates and assays for reliable testing of its activity were unavailable until recently, and most ..
  67. doi Identification of microRNAs regulated by isothiocyanates and association of polymorphisms inside their target sites with risk of sporadic colorectal cancer
    Ondrej Slaby
    Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic
    Nutr Cancer 65:247-54. 2013
    ..SNPs) localized within the 3'UTRs of genes (AGTR1, TNFAIP2, PRKCB, HSPA9, RABGAP1, DICER1, ADAM19, VWA5A, and SIRT5) targeted by these ITC-related miRNAs...
  68. pmc Genealogy of an ancient protein family: the Sirtuins, a family of disordered members
    Susan Costantini
    Pascale Foundation National Cancer Institute Cancer Research Center CROM, via Ammiraglio Bianco, 83013, Mercogliano, Italy
    BMC Evol Biol 13:60. 2013
    ..3D structures of human SIRT2, SIRT3, and SIRT5 revealed the overall conformation of the conserved core domain but they were unable to give a structural ..
  69. doi Proteomic analysis of mitochondrial proteins in cardiomyocytes from rats subjected to intermittent hypoxia
    Wei Zhong Zhu
    Physiological Laboratory of Hypoxia, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, 200031 Shanghai, People s Republic of China
    Eur J Appl Physiol 112:1037-46. 2012
    ..These findings will provide clues for understanding the mechanism of IH-induced cardiac protection and may lead to the development of interventional strategies designed to utilize the advantages of IH clinically...
  70. doi Sirtuins mediate mammalian metabolic responses to nutrient availability
    Angeliki Chalkiadaki
    Paul F Glenn Laboratory, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Building 68, Cambridge, MA 02139, USA
    Nat Rev Endocrinol 8:287-96. 2012
    ..The mitochondrial sirtuins, SIRT3, SIRT4 and SIRT5, regulate the activity of important mitochondrial enzymes and drive metabolic cycles in response to fasting and ..
  71. pmc Thiosuccinyl peptides as Sirt5-specific inhibitors
    Bin He
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    J Am Chem Soc 134:1922-5. 2012
    ..Here we demonstrate that thiosuccinyl peptides are potent and selective Sirt5 inhibitors...
  72. doi Substrates for efficient fluorometric screening employing the NAD-dependent sirtuin 5 lysine deacylase (KDAC) enzyme
    Andreas S Madsen
    Department of Chemistry, Technical University of Denmark, Kemitorvet 207, DK 2800, Kongens Lyngby, Denmark
    J Med Chem 55:5582-90. 2012
    ..substrates that enable highly efficient and enzyme-economical screening employing sirtuin 5 (SIRT5)...
  73. ncbi Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity
    R A Frye
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, 15240, USA
    Biochem Biophys Res Commun 260:273-9. 1999
    ..The SIRT1 sequence has the closest homology to the S. cerevisiae Sir2p. The SIRT4 and SIRT5 sirtuins more closely resemble prokaryotic sirtuin sequences...
  74. pmc Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins
    Eriko Michishita
    Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:4623-35. 2005
    ..with heterochromatic regions and nucleoli, respectively, where yeast Sir2 functions; 2) SIRT3, SIRT4, and SIRT5 are localized in mitochondria, an organelle that links aging and energy metabolism; 3) cellular p53 is a major in ..
  75. ncbi DNA pooling: a comprehensive, multi-stage association analysis of ACSL6 and SIRT5 polymorphisms in schizophrenia
    K V Chowdari
    Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Genes Brain Behav 6:229-39. 2007
    ..and two positional candidate genes: ACSL6 (Acyl-Coenzyme A synthetase long-chain family member 6) and SIRT5 (silent mating type information regulation 2 homologue 5)...
  76. ncbi Structural basis of inhibition of the human NAD+-dependent deacetylase SIRT5 by suramin
    Anja Schuetz
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada
    Structure 15:377-89. 2007
    ..We identified suramin as a compound that binds to human SIRT5 and showed that it inhibits SIRT5 NAD(+)-dependent deacetylase activity with an IC(50) value of 22 microM...
  77. ncbi Sirtuin functions in health and disease
    Hiroyasu Yamamoto
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue Laurent Fries, Boite Postale 10142, 67404 Illkirch, France
    Mol Endocrinol 21:1745-55. 2007
    ..Here, we review some of the recent data related to the sirtuins and discuss their mode of action, their biological role in cellular and organismal models, and their possible association to age-related human diseases...
  78. ncbi Structure-activity studies on suramin analogues as inhibitors of NAD+-dependent histone deacetylases (sirtuins)
    Johannes Trapp
    Institute of Pharmaceutical Sciences, Albert Ludwigs Universitat Freiburg, 79104 Freiburg, Germany
    ChemMedChem 2:1419-31. 2007
    ..The recently published X-ray crystal structure of human SIRT5 in complex with suramin and the human SIRT2 structure were used to analyze the interaction mode of the novel ..
  79. pmc Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation
    David B Lombard
    Howard Hughes Medical Institute, The Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 27:8807-14. 2007
    ..no mitochondrial hyperacetylation was detectable in mice lacking the two other mitochondrial sirtuins, SIRT4 and SIRT5. Surprisingly, despite this biochemical phenotype, SIRT3-deficient mice are metabolically unremarkable under basal ..
  80. ncbi Localization of mouse mitochondrial SIRT proteins: shift of SIRT3 to nucleus by co-expression with SIRT5
    Yasuhiko Nakamura
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 366:174-9. 2008
    ..regulator 2 (SIR2) is involved in extension of yeast longevity by calorie restriction, and SIRT3, SIRT4, and SIRT5 are mammalian homologs of SIR2 localized in mitochondria...
  81. doi Alcohol alters hepatic FoxO1, p53, and mitochondrial SIRT5 deacetylation function
    Charles S Lieber
    Section of Liver Disease and Nutrition, James J Peters VA Medical Center, 130 West Kingsbridge Road 151 2, Bronx, NY 10468, USA
    Biochem Biophys Res Commun 373:246-52. 2008
    ..04 and p=0.02, respectively) while hepatic p53 was found hyperacetylated (p=0.017). Furthermore, mitochondrial SIRT5 was reduced (p=0.0025), and PGC-1alpha hyperacetylated (p=0.027), establishing their role in protein modification...
  82. pmc Opposing effects of sirtuins on neuronal survival: SIRT1-mediated neuroprotection is independent of its deacetylase activity
    Jason A Pfister
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX, USA
    PLoS ONE 3:e4090. 2008
    ..Indeed, SIRT1 has been reported to protect against neuronal death, while SIRT2 promotes neurodegeneration. The effect of SIRTs 3-7 on the regulation of neuronal survival, if any, has yet to be reported...
  83. pmc SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span
    Tiara L A Kawahara
    Program in Epithelial Biology, Division of Endocrinology, Gerontology, and Metabolism, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 136:62-74. 2009
    ..We propose that SIRT6 attenuates NF-kappaB signaling via H3K9 deacetylation at chromatin, and hyperactive NF-kappaB signaling may contribute to premature and normal aging...
  84. pmc Ure(k)a! Sirtuins Regulate Mitochondria
    William C Hallows
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, 53706, USA
    Cell 137:404-6. 2009
    ..In this issue, Nakagawa et al. (2009) show that the sirtuin SIRT5 deacetylates and activates a mitochondrial enzyme, carbamoyl phosphate synthetase 1, which mediates the first step ..
  85. pmc SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle
    Takashi Nakagawa
    Department of Biology, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Cell 137:560-70. 2009
    ..Here, we show that SIRT5 localizes in the mitochondrial matrix and interacts with carbamoyl phosphate synthetase 1 (CPS1), an enzyme, ..
  86. pmc Proteome profile of functional mitochondria from human skeletal muscle using one-dimensional gel electrophoresis and HPLC-ESI-MS/MS
    Natalie Lefort
    Center for Metabolic Biology, Arizona State University, Tempe, AZ, USA
    J Proteomics 72:1046-60. 2009
    ..proteins were detected, and 487 of these were assigned to the mitochondrion, including the newly characterized SIRT5, MitoNEET and RDH13...
  87. doi Function and regulation of the mitochondrial sirtuin isoform Sirt5 in Mammalia
    Melanie Gertz
    Department of Physiological Chemistry, Ruhr University Bochum, Bochum, Germany
    Biochim Biophys Acta 1804:1658-65. 2010
    ..Mammals have seven Sirtuin isoforms, three of them - Sirt3, Sirt4, and Sirt5 - located to mitochondria, our centers of energy metabolism and apoptosis initiation...
  88. doi Mitochondrial sirtuins
    Jing Yi Huang
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    Biochim Biophys Acta 1804:1645-51. 2010
    ..Three sirtuins, SIRT3, 4 and 5, are located within the mitochondrial matrix. SIRT3 and SIRT5 are NAD(+)-dependent deacetylases that remove acetyl groups from acetyllysine-modified proteins and yield 2'-O-..
  89. doi Overexpression of SIRT5 confirms its involvement in deacetylation and activation of carbamoyl phosphate synthetase 1
    Masahito Ogura
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 393:73-8. 2010
    ..In mammals, among the seven SIR2 homologues (SIRT1-7), SIRT3, 4, and 5 are localized to mitochondria. As SIRT5 mRNA levels in liver are increased by fasting, the physiological role of SIRT5 was investigated in liver of SIRT5-..
  90. pmc Urea cycle regulation by mitochondrial sirtuin, SIRT5
    Takashi Nakagawa
    Paul F Glenn Laboratory for the Science of Aging and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Aging (Albany NY) 1:578-81. 2009
    Mammalian sirtuins have diverse roles in aging, metabolism and disease. Recently we reported a new function for SIRT5 in urea cycle regulation...
  91. pmc Sirtuin regulation of mitochondria: energy production, apoptosis, and signaling
    Eric Verdin
    Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA
    Trends Biochem Sci 35:669-75. 2010
    ..Three sirtuins, SIRT3, SIRT4 and SIRT5, are located in the mitochondria, dynamic organelles that function as the primary site of oxidative metabolism and ..
  92. pmc Brain molecular aging, promotion of neurological disease and modulation by sirtuin 5 longevity gene polymorphism
    Christin Glorioso
    Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Neurobiol Dis 41:279-90. 2011
    ..that subjects carrying a newly-characterized low-expressing polymorphism in a putative longevity gene (Sirtuin5; SIRT5(prom2)) have older brain molecular ages...
  93. doi The proteomic response of the mussel congeners Mytilus galloprovincialis and M. trossulus to acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress
    Lars Tomanek
    California Polytechnic State University, Department of Biological Sciences, Center for Coastal Marine Sciences, Environmental Proteomics Laboratory, 1 Grand Avenue, San Luis Obispo, CA 93407 0401, USA
    J Exp Biol 213:3559-74. 2010
    ..galloprovincialis, which is consistent with its expanding range in warmer waters...
  94. pmc Mechanism-based affinity capture of sirtuins
    Yana Cen
    Department of Pharmacology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA
    Org Biomol Chem 9:987-93. 2011
    ..that these tools are cross reactive against a variety of human sirtuin isoforms including SIRT1, SIRT2, SIRT3, SIRT5, SIRT6 and can react with microbial derived sirtuins as well...
  95. doi Zebrafish as a model organism to evaluate drugs potentially able to modulate sirtuin expression
    Talita Carneiro Brandão Pereira
    Laboratório de Biologia Genômica e Molecular, Departamento de Biologia Celular e Molecular, Faculdade de Biociencias, Pontificia Universidade Catolica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
    Zebrafish 8:9-16. 2011
    ..SIRT1, SIRT3, SIRT5, and SIRT6 genes were expressed in all tissues, and SIRT1 exhibited the highest level of expression in all organs...
  96. doi Histone deacetylase inhibitors demonstrate significant preclinical activity as single agents, and in combination with bortezomib in Waldenström's macroglobulinemia
    Jenny Y Sun
    Bing Center for Waldenstrom s Macroglobulinemia, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Clin Lymphoma Myeloma Leuk 11:152-6. 2011
    ..of bone marrow CD19+ cells from 30 patients and 10 healthy donors showed overexpression of HDAC4, HDAC9, and Sirt5, with validation of HDAC9 overexpression by q-PCR in primary and BCWM.1 cells...
  97. ncbi Advances in characterization of human sirtuin isoforms: chemistries, targets and therapeutic applications
    Y Cen
    Department of Pharmacology, Weill Medical College of Cornell University, New York, NY 10065, USA
    Curr Med Chem 18:1919-35. 2011
    ..There are seven human sirtuin isoforms called SIRT1-SIRT7. The biochemical functions of SIRT1, SIRT2, SIRT3, SIRT5 and SIRT6 have been reported and NAD+ dependent deacetylase activities confirmed...
  98. pmc Mammalian sirtuins and energy metabolism
    Xiaoling Li
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Int J Biol Sci 7:575-87. 2011
    ..This review focuses on the involvement of two nuclear sirtuins, SIRT1 and SIRT6, and three mitochondrial sirtuins, SIRT3, SIRT4, and SIRT5, in regulation of diverse metabolic processes.
  99. doi SIRT1 and SIRT5 activity expression and behavioral responses to calorie restriction
    Yi Qun Geng
    Central Laboratory, Shantou University and The Chinese University of Hong Kong Joint Shantou International Eye Center, Shantou, Guangdong 515041, China
    J Cell Biochem 112:3755-61. 2011
    To investigate the effects of calorie restriction (CR) on behavioral performance and expression of SIRT1 and SIRT5 in rat cerebral tissues...
  100. pmc Mitochondrial sirtuins in the regulation of mitochondrial activity and metabolic adaptation
    David B Lombard
    Department of Pathology, Institute of Gerontology, 3015 BSRB, Ann Arbor, MI 48109, USA
    Handb Exp Pharmacol 206:163-88. 2011
    ..In mammals, three members of the sirtuin class of NAD(+)-dependent deacetylases - SIRT3, SIRT4, and SIRT5 - localize to mitochondria and regulate targets involved in a diverse array of biochemical pathways...
  101. pmc Old enzymes, new tricks: sirtuins are NAD(+)-dependent de-acylases
    Matthew D Hirschey
    Sarah W Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, NC 27710, USA
    Cell Metab 14:718-9. 2011
    ..Two new studies by Du et al. (2011) and Peng et al. (2011) identify a new enzymatic activity for SIRT5, expanding the cellular repertoire of posttranslational modifications targeted by the sirtuins.

Research Grants19

  1. Eric S Goetzman; Fiscal Year: 2016
    ..Specific Aim 1 will investigate interactions between the mitochondrial sirtuins Sirt3, Sirt4, and Sirt5 and the enzymes very long-chain acyl-CoA dehydrogenase (VLCAD), medium chain-acyl-CoA dehydrogenase (MCAD), and ..
  2. High-throughput assays for the development of SIRT5-specific inhibitors
    Hening Lin; Fiscal Year: 2012
    ..Our laboratory has recently demonstrated that SIRT5, which has very weak deacetylase activity, can catalyze the hydrolysis of malonyl and succinyl groups from lysine ..
  3. DAVID BENNER LOMBARD; Fiscal Year: 2016
    ..The application is based on two novel observations. First, the mitochondrial sirtuin SIRT5 plays a hitherto undescribed role in deacetylating and suppressing activity of Pyruvate Dehydrogenase Complex (PDC)..
  4. Chemical Tools to Visualize Sirtuin Activity in Cells
    Anthony A Sauve; Fiscal Year: 2013
    ..For example, sirtuins are naturally compartmentalized;SIRT1, SIRT6 and SIRT7 are nuclear, whereas SIRT3, SIRT4 and SIRT5 are mitochondrial. SIRT2 is cytosolic...
  5. Kelly E Sullivan; Fiscal Year: 2015
    ..As a long-term goal, the ability of KGA to contribute to transformation and regulate GAC activity will be investigated. ..
  6. Etienne L Sibille; Fiscal Year: 2015
    ..This will provide (iii) evidence-based hypotheses to monitor critical functional domains in old age, and (iv) biological leads for rational experimental design to investigate successful aging. ..
  7. Robert S Weiss; Fiscal Year: 2015
    ..These modifications appear to be regulated by the mitochondrial protein Sirt5, a member of the Sir2 family of proteins (for Silent information regulator 2)...
  8. Robert T Kennedy; Fiscal Year: 2015
    ..Important innovations include segmented flow sample introduction for MS and electrophoresis, high-throughput sample preparation, and development of assays for novel protein targets. ..
  9. Synergistic Nutraceutical Effects of DLPC and SAMe
    Charles Lieber; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  10. ROLE OF CHROMATIN IN HIV TRANSCRIPTIONAL REGULATION
    ERIC M VERDIN; Fiscal Year: 2010
    ..These experiments have the potential of identifying novel factors mediating HIV transcriptional regulation that could be targeted therapeutically in the future. ..
  11. MODULATION OF T CELL FUNCTIONS BY HIV1 TAT
    Eric Verdin; Fiscal Year: 2001
    ..These studies will further define the role of Tat as modulator of T cell differentiated functions and establish its role in HIV pathogenesis. ..
  12. Antiviral/Antifibrotic Liver Therapy of Hepatitis C positive Alcohol Drinkers
    Charles Lieber; Fiscal Year: 2007
    ..Accordingly, support is requested for the completion of this novel approach for the treatment of HCV+ patients who become abstinent or who continue to consume alcohol moderately. [unreadable] [unreadable]..
  13. Regulation of HIV transcription and replication by SDF-1gamma
    Eric Verdin; Fiscal Year: 2006
    ..We will determine whether a correlations exist between SDF-1gamma expression, HIV replication and the presence of the SDF1-3'A allele. ..
  14. Liver Fibrosis, Inflammation & Oxidative Stress Markers
    Charles Lieber; Fiscal Year: 2006
    ..abstract_text> ..
  15. ANTIVIRAL & ANTIFIBROTIC LIVER THERAPY OF HCV+ DRINKERS
    Charles Lieber; Fiscal Year: 2005
    ..Funds are requested for special laboratory tests, study nurses, travel to meetings, patient monitoring expenses and a core office. ..
  16. DLPC Treatment of Alcoholic and Non-Alcoholic Fibrosis
    Charles Lieber; Fiscal Year: 2006
    ..abstract_text> ..
  17. A New In Vitro Model for HIV Latency
    Eric Verdin; Fiscal Year: 2004
    ..These studies should further establish the validity of our model for the study of HIV latency in patients. ..
  18. MODULATION OF CD8 T CELL FUNCTION BY HIV ENVELOPE PROTEI
    Eric Verdin; Fiscal Year: 2003
    ..abstract_text> ..
  19. MECHANISM AND ROLE OF MICROSOMAL ETHANOL OXIDATION
    Charles Lieber; Fiscal Year: 2001
    ..Such information is aimed at elucidating key aspects of alcohol mediated pathology in a way which may eventually affect alcohol related medical complications, their prevention and treatment. ..