SDHA

Summary

Gene Symbol: SDHA
Description: succinate dehydrogenase complex flavoprotein subunit A
Alias: CMD1GG, PGL5, SDH1, SDH2, SDHF, succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, flavoprotein subunit of complex II, succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Species: human

Top Publications

  1. pmc Selection of reliable reference genes for qPCR studies on chondroprotective action
    Stefan Toegel
    Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A 1090 Vienna, Austria
    BMC Mol Biol 8:13. 2007
  2. ncbi Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency
    T Bourgeron
    Unite de Recherches sur les Handicaps Genetiques de l Enfant, INSERM U 393, Institut Necker, Hopital des Enfants Malades, Paris, France
    Nat Genet 11:144-9. 1995
  3. ncbi Compound heterozygous mutations in the flavoprotein gene of the respiratory chain complex II in a patient with Leigh syndrome
    B Parfait
    INSERM U393, Hopital Necker Enfants Malades, Paris, France
    Hum Genet 106:236-43. 2000
  4. ncbi A role for mitochondrial enzymes in inherited neoplasia and beyond
    Charis Eng
    Clinical Cancer Genetics Program, Comprehensive Cancer Center, and Division of Human Genetics, Department of Internal Medicine, The Ohio State University, 420 W 12th Avenue, Ste 690 TMRF, Columbus, Ohio 43210, USA
    Nat Rev Cancer 3:193-202. 2003
  5. ncbi Homozygous Gly555Glu mutation in the nuclear-encoded 70 kDa flavoprotein gene causes instability of the respiratory chain complex II
    Rudy Van Coster
    Department of Pediatrics, Division of Pediatric Neurology and Metabolism, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Am J Med Genet A 120:13-8. 2003
  6. ncbi Succinate dehydrogenase deficiency in human
    J J Brière
    INSERM U676, Batiment Ecran, Hopital Robert Debre, 48, boulevard Serurier, 75019, Paris, France
    Cell Mol Life Sci 62:2317-24. 2005
  7. pmc Leigh syndrome caused by mutations in the flavoprotein (Fp) subunit of succinate dehydrogenase (SDHA)
    R Horvath
    Metabolic Disease Centre Munich Schwabing, Institute of Clinical Chemistry, Academic Hospital Schwabing, Kolner Platz 1, 80804 Munich, Germany
    J Neurol Neurosurg Psychiatry 77:74-6. 2006
  8. pmc SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma
    Huai Xiang Hao
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Science 325:1139-42. 2009
  9. pmc Familial neonatal isolated cardiomyopathy caused by a mutation in the flavoprotein subunit of succinate dehydrogenase
    Aviva Levitas
    Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Eur J Hum Genet 18:1160-5. 2010
  10. doi SDHA loss-of-function mutations in KIT-PDGFRA wild-type gastrointestinal stromal tumors identified by massively parallel sequencing
    Maria A Pantaleo
    Department of Hematology and Oncological Sciences L A Seragnoli, Sant Orsola Malpighi Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy
    J Natl Cancer Inst 103:983-7. 2011

Research Grants

  1. MOLEC GENETICS OF COMPLEX I IN MAMMALIAN MITOCHONDRIA
    Immo Scheffler; Fiscal Year: 2004
  2. Using Synthetic Lethality to Select Cancer Drug Targets
    William Kaelin; Fiscal Year: 2005
  3. 2006 Cancer Model Mechanisms
    William Kaelin; Fiscal Year: 2006
  4. Regulation of Streptococcus pyogenes exoproteins
    MICHAEL CHAUSSEE; Fiscal Year: 2007
  5. O2 Sensing in Hypoxic Pulmonary Vasoconstriction
    Paul Schumacker; Fiscal Year: 2009
  6. Proximal determinants of risk for tularemia outbreaks
    Sam Telford; Fiscal Year: 2004
  7. Perpetuation of Francisella tularensis
    Sam R Telford; Fiscal Year: 2010
  8. OXYGEN SENSING AND CELL SIGNALING IN HYPOXIA
    Paul Schumacker; Fiscal Year: 2001
  9. O2 SENSING BY MITOCHONDRIA DURING INTERMITTENT HYPOXIA
    Paul Schumacker; Fiscal Year: 2003
  10. Real-Time Imaging of Hypoxia based on VHL Activity
    William Kaelin; Fiscal Year: 2006

Detail Information

Publications180 found, 100 shown here

  1. pmc Selection of reliable reference genes for qPCR studies on chondroprotective action
    Stefan Toegel
    Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A 1090 Vienna, Austria
    BMC Mol Biol 8:13. 2007
    ..GAPDH, Glyceraldehyde-3-phosphate; B2M, Beta-2-microglobulin; HPRT1, Hypoxanthine phosphoribosyl-transferase I; SDHA, Succinate dehydrogenase complex, subunit A; YWHAZ, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation ..
  2. ncbi Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency
    T Bourgeron
    Unite de Recherches sur les Handicaps Genetiques de l Enfant, INSERM U 393, Institut Necker, Hopital des Enfants Malades, Paris, France
    Nat Genet 11:144-9. 1995
    ..This is the first report of a nuclear gene mutation causing a mitochondrial respiratory chain deficiency in humans...
  3. ncbi Compound heterozygous mutations in the flavoprotein gene of the respiratory chain complex II in a patient with Leigh syndrome
    B Parfait
    INSERM U393, Hopital Necker Enfants Malades, Paris, France
    Hum Genet 106:236-43. 2000
    ..So far, profound deficiencies in complex II activity resulting from mutations in the Fp gene of the SDH present only as LS, a striking observation in view of the ubiquitous expression of this typical housekeeping gene in humans...
  4. ncbi A role for mitochondrial enzymes in inherited neoplasia and beyond
    Charis Eng
    Clinical Cancer Genetics Program, Comprehensive Cancer Center, and Division of Human Genetics, Department of Internal Medicine, The Ohio State University, 420 W 12th Avenue, Ste 690 TMRF, Columbus, Ohio 43210, USA
    Nat Rev Cancer 3:193-202. 2003
    ..Understanding this link between inherited cancer syndromes and neurological disease could provide further insights into the mechanisms by which mitochondrial deficiencies lead to tumour development...
  5. ncbi Homozygous Gly555Glu mutation in the nuclear-encoded 70 kDa flavoprotein gene causes instability of the respiratory chain complex II
    Rudy Van Coster
    Department of Pediatrics, Division of Pediatric Neurology and Metabolism, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Am J Med Genet A 120:13-8. 2003
    ..This observation prompts speculation of a labile interaction between Ip and Fp polypeptides and of a key role of the amino acid at position 555 in the interacting domain...
  6. ncbi Succinate dehydrogenase deficiency in human
    J J Brière
    INSERM U676, Batiment Ecran, Hopital Robert Debre, 48, boulevard Serurier, 75019, Paris, France
    Cell Mol Life Sci 62:2317-24. 2005
    ..Mutations in the four genes, SDHA, B, C and D, have been reported, resulting in strikingly diverse clinical presentations...
  7. pmc Leigh syndrome caused by mutations in the flavoprotein (Fp) subunit of succinate dehydrogenase (SDHA)
    R Horvath
    Metabolic Disease Centre Munich Schwabing, Institute of Clinical Chemistry, Academic Hospital Schwabing, Kolner Platz 1, 80804 Munich, Germany
    J Neurol Neurosurg Psychiatry 77:74-6. 2006
    ..Biochemical measurement of skeletal muscle showed a severe decrease in mitochondrial complex II. Sequencing of SDHA revealed compound heterozygosity for a nonsense mutation in exon 4 (W119X) and a missense mutation in exon 3 (A83V),..
  8. pmc SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma
    Huai Xiang Hao
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Science 325:1139-42. 2009
    ..Sdh5 is required for SDH-dependent respiration and for Sdh1 flavination (incorporation of the flavin adenine dinucleotide cofactor)...
  9. pmc Familial neonatal isolated cardiomyopathy caused by a mutation in the flavoprotein subunit of succinate dehydrogenase
    Aviva Levitas
    Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Eur J Hum Genet 18:1160-5. 2010
    ..In this study, we present the association of a mutation in the SDHA gene with recessive neonatal isolated DCM in 15 patients of two large consanguineous Bedouin families...
  10. doi SDHA loss-of-function mutations in KIT-PDGFRA wild-type gastrointestinal stromal tumors identified by massively parallel sequencing
    Maria A Pantaleo
    Department of Hematology and Oncological Sciences L A Seragnoli, Sant Orsola Malpighi Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy
    J Natl Cancer Inst 103:983-7. 2011
    ..mutations in SDHB and SDHC (which encode succinate dehydrogenase subunits B and C, respectively) but not in SDHA and SDHD (which encode subunits A and D, respectively) were identified in KIT/PDGFRA wild-type GISTs...
  11. pmc The SDH mutation database: an online resource for succinate dehydrogenase sequence variants involved in pheochromocytoma, paraganglioma and mitochondrial complex II deficiency
    Jean Pierre Bayley
    Department of Human Genetics, Center for Human and Clinical Genetics, Leiden University Medical Center, P, O, Box 9503, 2300 RA, Leiden, The Netherlands
    BMC Med Genet 6:39. 2005
    The SDHA, SDHB, SDHC and SDHD genes encode the subunits of succinate dehydrogenase (succinate: ubiquinone oxidoreductase), a component of both the Krebs cycle and the mitochondrial respiratory chain...
  12. ncbi Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii
    R A Heinzen
    Department of Microbiology, Washington State University, Pullman 99164 4233, USA
    Gene 155:27-34. 1995
    ..6% for SdhD to a high of 61.2% for SdhA and SdhB. Primer extension identified transcription start points (tsp) for sdh and sucA...
  13. pmc A proteomic study of Corynebacterium glutamicum AAA+ protease FtsH
    Alja Lüdke
    Institut fur Biochemie, Universitat zu Koln, Zülpicher Str, 47, 50674 Koln, Germany
    BMC Microbiol 7:6. 2007
    ..For the analysis of the membrane fraction, anion exchange chromatography was combined with SDS-PAGE, while the cytoplasmic protein fraction was studied by conventional two-dimensional gel electrophoresis...
  14. ncbi Fumarate reductase and succinate oxidase activity of Escherichia coli complex II homologs are perturbed differently by mutation of the flavin binding domain
    Elena Maklashina
    Molecular Biology Division, Veterans Affairs Medical Center, San Francisco, California 94121, USA
    J Biol Chem 281:11357-65. 2006
    ..there is a Glu (FrdA Glu-49) near the covalently bound FAD cofactor in most QFRs, which is replaced with a Gln (SdhA Gln-50) in SQRs...
  15. ncbi Preservation of RNA and destruction of infectivity in microdissected brain tissues of Lewis rats infected with the Borna disease virus
    Doris Porombka
    Department of Pathology, University of Veterinary Medicine Hannover, Bunteweg 17, 30559 Hannover, Germany
    J Virol Methods 135:247-53. 2006
    ..BDV-N) and the cellular genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), succinate-ubiquinone reductase (SDHA) and hypoxanthine phosphoribosyl-transferase-1 (HPRT) was measured by real-time RT-PCR and compared to BDV-infected ..
  16. doi Biochemical and biophysical characterization of succinate: quinone reductase from Thermus thermophilus
    Olga Kolaj-Robin
    Chemical and Environmental Sciences Department and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland
    Biochim Biophys Acta 1807:68-79. 2011
    ..It consists of four polypeptides with apparent molecular masses of 64, 27, 14 and 15kDa, corresponding to SdhA (flavoprotein), SdhB (iron-sulfur protein), SdhC and SdhD (membrane anchor proteins), respectively...
  17. pmc The dual-functioning fumarate reductase is the sole succinate:quinone reductase in Campylobacter jejuni and is required for full host colonization
    Rebecca A Weingarten
    Department of Microbiology, North Carolina State University, Campus Box 7615, Raleigh, NC 27695, USA
    J Bacteriol 191:5293-300. 2009
    ..The sdhA::cat(+) strain exhibited wild-type levels of succinate dehydrogenase activity both in vivo and in vitro...
  18. pmc Nuclear SDH2-1 and SDH2-2 genes, encoding the iron-sulfur subunit of mitochondrial complex II in Arabidopsis, have distinct cell-specific expression patterns and promoter activities
    Alvaro Elorza
    Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Casilla 114 D, Santiago, Chile
    Plant Physiol 136:4072-87. 2004
    ..To find clues about their role, we have undertaken a detailed analysis of their expression. Two genes (SDH2-1 and SDH2-2) that likely arose via a relatively recent duplication event are expressed in all organs from adult ..
  19. ncbi A rapid method for gene expression analysis of Borna disease virus in neurons and astrocytes using laser microdissection and real-time RT-PCR
    Doris Porombka
    Department of Pathology, University of Veterinary Medicine Hannover, Bunteweg 17, D 30559 Hannover, Germany
    J Virol Methods 148:58-65. 2008
    ..different reference genes (glyceraldehyde-3-phosphate dehydrogenase [GAPDH], succinate-ubiquinone reductase [SDHA], hypoxanthine phosphoribosyl-transferase-1 [HPRT]) were tested...
  20. pmc Gene mutations in the succinate dehydrogenase subunit SDHB cause susceptibility to familial pheochromocytoma and to familial paraganglioma
    D Astuti
    Section of Medical and Molecular Genetics, Department of Paediatrics and Child Health, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Am J Hum Genet 69:49-54. 2001
    ..of two components of succinate dehydrogenase, SDHC and SDHD, anchor the gene products of two other components, SDHA and SDHB, which form the catalytic core, to the inner-mitochondrial membrane...
  21. doi Functional study in a yeast model of a novel succinate dehydrogenase subunit B gene germline missense mutation (C191Y) diagnosed in a patient affected by a glomus tumor
    Paola Goffrini
    Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, Parma, Italy
    Hum Mol Genet 18:1860-8. 2009
    ..The pathogenic significance of the human mutation was validated in a yeast model. SDH2(C184Y) mutant allele equivalent to human SDHB(C191Y) did not restore the OXPHOS phenotype of the Deltasdh2 null ..
  22. ncbi Insertion of transposon Tn5tac1 in the Sinorhizobium meliloti malate dehydrogenase (mdh) gene results in conditional polar effects on downstream TCA cycle genes
    Sergiy I Dymov
    Department of Natural Resource Sciences, McGill University, QC, Canada
    Mol Plant Microbe Interact 17:1318-27. 2004
    ..Cosmid clone pDS15 restored MDH activity to Rm30049, complemented both the mutant growth and symbiotic phenotypes, and was found to carry six complete (sdhB, mdh, sucCDAB) and two partial (IpdA, sdhA) tricarboxylic acid cycle genes.
  23. pmc A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II is regulated by B3 domain transcription factors during seed development in Arabidopsis
    Hannetz Roschzttardtz
    Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Plant Physiol 150:84-95. 2009
    ..One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the ..
  24. ncbi Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana
    P Figueroa
    Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, P Universidad Catolica de Chile, Santiago
    Plant Mol Biol 46:241-50. 2001
    ..In Arabidopsis thaliana, the homologous protein is now found to be encoded by three nuclear genes. Two genes (sdh2-1 and sdh2-2) likely arose from a relatively recent duplication event since they have similar structures, encode ..
  25. pmc Pervasive survival of expressed mitochondrial rps14 pseudogenes in grasses and their relatives for 80 million years following three functional transfers to the nucleus
    Han Chuan Ong
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    BMC Evol Biol 6:55. 2006
    ..the rps14 gene of three grasses (rice, maize, and wheat), which has been relocated to the intron of the nuclear sdh2 gene and which is expressed and targeted to the mitochondrion via alternative splicing and usage of the sdh2 ..
  26. pmc The triad of paragangliomas, gastric stromal tumours and pulmonary chondromas (Carney triad), and the dyad of paragangliomas and gastric stromal sarcomas (Carney-Stratakis syndrome): molecular genetics and clinical implications
    C A Stratakis
    Section on Endocrinology and Genetics, Program on Developmental Endocrinology and Genetics, NICHD, NIH, Building 10, CRC, Room 1 3330, 10 Center Dr, MSC1103, Bethesda, MD 20892, USA
    J Intern Med 266:43-52. 2009
    ..series of patients with CT, 34 females and three males (median age of presentation 21 years) who did not carry SDHA, SDHB, SDHC, SDHD, KIT or PDGFRA gene mutations...
  27. ncbi The Saccharomyces cerevisiae succinate-ubiquinone reductase contains a stoichiometric amount of cytochrome b562
    K S Oyedotun
    The Medical Research Council of Canada Group in the Molecular Biology of Membranes, Department of Biochemistry, University of Alberta, Edmonton
    FEBS Lett 442:203-7. 1999
    ..reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes. In most organisms, SDH contains one or two endogenous b-type hemes...
  28. pmc Genes encoding the same three subunits of respiratory complex II are present in the mitochondrial DNA of two phylogenetically distant eukaryotes
    G Burger
    Program in Evolutionary Biology, Canadian Institute for Advanced Research, Departement de Biochimie, Universite de Montreal, Quebec, Canada
    Proc Natl Acad Sci U S A 93:2328-32. 1996
    ..These complex II genes, sdh2, sdh3, and sdh4, are homologs, respectively, of Escherichia coli sdhB, sdhC, and sdhD. In E...
  29. pmc Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model
    Kristina Langnaese
    Institute of Medical Neurobiology, University of Magdeburg, Leipziger Str, 44, D 39120 Magdeburg, Germany
    BMC Mol Biol 9:53. 2008
    ..hypoxanthine guanine phosphoribosyl transferase; Pgk1, phosphoglycerate kinase 1; Rpl13a, ribosomal protein L13A; Sdha, succinat dehydrogenase complex, subunit a, flavoprotein (Fp); Tbp, TATA box binding protein; Ywhaz, tyrosine 3-..
  30. doi Subchronic exposure to arsenic decreased Sdha expression in the brain of mice
    Yan Hong
    Department of Occupational and Environmental Health, Dalian Medical University, No 9 Western Section of Lushun South Road, Dalian, Liaoning 116044, China
    Neurotoxicology 30:538-43. 2009
    ..Our results showed that immunoreactivity of 8-OHdG increased remarkably. Succinate dehydrogenase subunit A (Sdha), ubiquinol-cytochrome c oxidoreductase gene (Uqcr), cytochrome oxidase genes (Cox6a2, Cox17) and ATP Synthase ..
  31. doi Succinate dehydrogenase in Arabidopsis thaliana is regulated by light via phytochrome A
    Vasily N Popov
    Department of Biochemistry and Cell Physiology, Voronezh State University, Voronezh, Russia
    FEBS Lett 584:199-202. 2010
    ..The white or red light were found to be down-regulating factors for the mRNA content of the sdh1-2 and sdh2-3 genes and SDH catalytic activity both in A...
  32. ncbi Cytopathies involving mitochondrial complex II
    Brian A C Ackrell
    Department of Veterans Affairs Medical Center, Molecular Biology Division, San Francisco, CA 94121, USA
    Mol Aspects Med 23:369-84. 2002
    ..oxidoreductase) is the smallest complex in the respiratory chain and contains four nuclear-encoded subunits SdhA, SdhB, SdhC, and SdhD. It functions both as a respiratory chain component and an essential enzyme of the TCA cycle...
  33. pmc Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells
    Claude A Piantadosi
    Department of Medicine, Duke University School of Medicine and the Durham Veteran s Administration Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 283:10967-77. 2008
    ..NRF-1 was found to bind to the gene promoters of two of four nuclear-encoded Complex II subunits: SDHa and SDHd, but the enzyme activity was dynamically regulated through the catalytic SDHa flavoprotein...
  34. doi Validation of adequate endogenous reference genes for the normalisation of qPCR gene expression data in human post mortem tissue
    Antje Koppelkamm
    Institute of Legal Medicine, University of Freiburg, Albertstr 9, 79104, Freiburg, Germany
    Int J Legal Med 124:371-80. 2010
    ..Considering cardiac muscle tissue, CYCA and TBP were identified as the most stable while in skeletal muscle tissue, SDHA and TBP, and in brain tissue, SDHA and HMBS turned out to be the most stable...
  35. ncbi [Dental knowledge and evaluation of school dental care by school graduates in Berne canton]
    O Kronenberg
    Universitat Bern, Klinik für Zahnerhaltung, Präventiv und Kinderzahnmedizin, Freiburgstr 7, 3010 Bern
    Schweiz Monatsschr Zahnmed 111:948-56. 2001
    ..Besides parents and the family, dentists and specifically trained auxiliaries (school dental health assistants, SDHA) were found to play a central role in the mediation of theoretical knowledge and in the practical instruction of ..
  36. pmc Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue
    David T R Coulson
    Division of Psychiatry and Neuroscience, School of Medicine and Dentistry, Queen s University Belfast, Whitla Medical Building, Belfast, BT9 7BL, Northern Ireland, UK
    BMC Mol Biol 9:46. 2008
    ..Given that expression of some commonly used reference genes is altered in certain conditions, this study aimed to establish which reference genes were stably expressed in post mortem brain tissue from individuals with AD, PD or DLB...
  37. doi Transcription of Corynebacterium glutamicum genes involved in tricarboxylic acid cycle and glyoxylate cycle
    Sung Ok Han
    Research Institute of Innovative Technology for the Earth, Kyoto, Japan
    J Mol Microbiol Biotechnol 15:264-76. 2008
    ..Northern hybridizations revealed that gltA-fkb, odhA-orfA, sucC-sucD, sdhC-sdhA-sdhB-orfB and mdh-orfC were transcribed as polycistronic mRNAs of size 1.9, 4.5, 2.5, 4.0 and 1.7 kb, respectively...
  38. pmc Diversity of Francisella species in environmental samples from Martha's Vineyard, Massachusetts
    Zenda L Berrada
    Tufts Cummings School of Veterinary Medicine, North Grafton, MA, USA
    Microb Ecol 59:277-83. 2010
    ..by polymerase chain reaction (PCR) targeting Francisella species 16S rRNA gene and succinate dehydrogenase A (sdhA) sequences; evidence of the agent of tularemia was sought by amplification of Francisella tularensis-specific ..
  39. pmc Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
    Nicholas Silver
    Molecular Haematology, Division of Gene and Cell Based Therapy, King s College London School of Medicine at King s College Hospital, Denmark Hill, London, SE5 9PJ, UK
    BMC Mol Biol 7:33. 2006
    ..On this basis, stability of the candidate housekeeping genes was ranked according to repeatability of the gene expression differences among 31 samples...
  40. ncbi Cellular responses and proteomic analysis of Escherichia coli exposed to green tea polyphenols
    Y S Cho
    Department of Biotechnology, Soonchunhyang University, PO Box 97, Asan, Chung Nam 336 600, Republic of Korea
    Curr Microbiol 55:501-6. 2007
    ..was downregulated by exposure to TPP (including proteins involved in carbon and energy metabolism, such as Eno, SdhA, and UgpQ, as well as those involved in amino-acid biosynthesis, such as GltK and TyrB)...
  41. ncbi A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination
    Alvaro Elorza
    Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, P Universidad Catolica de Chile, Casilla 114 D, Santiago
    Plant Cell Physiol 47:14-21. 2006
    Three nuclear genes, SDH2-1, SDH2-2 and SDH2-3, encode the essential iron-sulfur subunit of mitochondrial complex II in Arabidopsis thaliana...
  42. ncbi Physical and transcript map of the dominant optic atrophy (OPA1) gene critical region at 3q28-q29
    J T Behnam
    Division of Medical and Molecular Genetics, Guy s Hospital, London, SE1 9RT, United Kingdom
    Genomics 72:213-6. 2001
    ..We found the OPA1 critical interval to be 450-550 kb. It contains 2 known genes, RPL35a and SDHA, which thus constitute candidate genes.
  43. pmc Reliable transcript quantification by real-time reverse transcriptase-polymerase chain reaction in primary neuroblastoma using normalization to averaged expression levels of the control genes HPRT1 and SDHA
    Matthias Fischer
    University Children s Hospital Cologne, Department of Pediatric Oncology, Joseph Stelzmann Str 9, 50924 Cologne, Germany
    J Mol Diagn 7:89-96. 2005
    ..stability of six supposed control genes was evaluated in 64 samples of primary neuroblastoma and HPRT1 and SDHA mRNA levels were shown to exhibit the least expression variability among the samples...
  44. ncbi Quality of life and prognosis in heart failure: results of the Beta-Blocker Evaluation of Survival Trial (BEST)
    Charles W Tate
    Division of Cardiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Card Fail 13:732-7. 2007
    ..The goal of the current study was to determine the relationship between the different questionnaires, assess the effect of treatment on health status, and evaluate the association between changes in health status and prognosis...
  45. pmc Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis
    Stefan Cikos
    Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4, 04001 Kosice, Slovakia
    BMC Mol Biol 8:113. 2007
    ..Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity of target mRNA. The method chosen for data analysis can strongly influence results of the quantification...
  46. ncbi The Saccharomyces cerevisiae TCM62 gene encodes a chaperone necessary for the assembly of the mitochondrial succinate dehydrogenase (complex II)
    E Dibrov
    Medical Research Council of Canada Group in the Molecular Biology of Membranes, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 273:32042-8. 1998
    ..SDH is encoded by the SDH1, SDH2, SDH3, and SDH4 genes. The holoenzyme is composed of two domains...
  47. ncbi Transcriptome analysis of acetate metabolism in Corynebacterium glutamicum using a newly developed metabolic array
    Mikiro Hayashi
    Tokyo Research Laboratories, Kyowa Hakko Kogyo Co, Ltd, Machida, Japan
    Biosci Biotechnol Biochem 66:1337-44. 2002
    ..For example, several TCA cycle genes, such as gltA, sdhA, sdhB, fumH, and mdh, and the gluconeogenic gene pck were up-regulated in the acetate medium...
  48. pmc Functional interactions between the carbon and iron utilization regulators, Crp and Fur, in Escherichia coli
    Zhongge Zhang
    Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0116, USA
    J Bacteriol 187:980-90. 2005
    ..Several other genes including the sdhA-D, sucA-D, and fumA genes, encoding key constituents of the Krebs cycle, proved to be repressed by the loss of both ..
  49. doi The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme
    L M Garcia
    Departamento de Bioquimica, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico, DF Mexico
    Can J Microbiol 54:456-66. 2008
    ..cereus belongs to the type-B group of SQR enzymes, consisting of a 65-kDa flavoprotein (SdhA), a 29-kDa iron-sulphur protein (SdhB), and a 19-kDa subunit containing 2 b-type cytochromes (SdhC)...
  50. doi Identification of suitable normalizing genes for quantitative real-time RT-PCR analysis of gene expression in fetal mouse gonads
    T Svingen
    Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
    Sex Dev 3:194-204. 2009
    ..5 to 14.5 dpc irrespective of sex. Based on our analysis, we suggest that Rn18s, Rps29, Tbp and Sdha are suitable reference genes for qRT-PCR expression studies during early gonad differentiation in the mouse.
  51. doi Patients with Leber hereditary optic neuropathy fail to compensate impaired oxidative phosphorylation
    Alex Korsten
    Department of Neurology, Erasmus MC Rotterdam, The Netherlands
    Biochim Biophys Acta 1797:197-203. 2010
    ..An increase in mitochondrial density per cell compensated for the dysfunctional mitochondria in LHON carriers but was insufficient to result in a normal biochemical phenotype in early-onset LHON patients...
  52. doi Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive D-galactose pathway and essential for D-sorbitol catabolism
    Outi M Koivistoinen
    VTT Technical Research Centre of Finland, Finland
    FEBS Lett 586:378-83. 2012
    ..We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in D-galactose and D-..
  53. pmc SdhE is a conserved protein required for flavinylation of succinate dehydrogenase in bacteria
    Matthew B McNeil
    Department of Microbiology and Immunology, University of Otago, Dunedin 9054, New Zealand
    J Biol Chem 287:18418-28. 2012
    ..SdhE interacted with the flavoprotein SdhA, directly bound the flavin adenine dinucleotide co-factor, and was required for the flavinylation of SdhA...
  54. doi Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat model
    Manuela Cabiati
    Laboratory of Cardiovascular Biochemistry, CNR Institute of Clinical Physiology, Via Giuseppe Moruzzi 1, 56124 Pisa, Italy
    J Mol Endocrinol 48:251-60. 2012
    ..The aim of this study was to select among ten candidates (Actb, Gapdh, Polr2a, Ywhag, Rpl13a, Sdha, Ppia, Tbp, Hprt1 and Tfrc) a set of reference genes that can be used for the normalization of mRNA expression data ..
  55. doi Succinate dehydrogenase (SDH) and mitochondrial driven neoplasia
    Anthony J Gill
    Department of Anatomical Pathology and Northern Cancer Translational Research Unit, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Pathology 44:285-92. 2012
    The genes for the succinate dehydrogenase (SDH) subunits SDHA, SDHB, SDHC and SDHD are encoded in the autosome...
  56. doi Somatic NF1 inactivation is a frequent event in sporadic pheochromocytoma
    Nelly Burnichon
    INSERM, UMR970, Paris Cardiovascular Research Center, Paris, France
    Hum Mol Genet 21:5397-405. 2012
    Germline mutations in the RET, SDHA, SDHAF2, SDHB, SDHC, SDHD, MAX, TMEM127, NF1 or VHL genes are identified in about 30% of patients with pheochromocytoma or paraganglioma and somatic mutations in RET, VHL or MAX genes are reported in 17%..
  57. pmc SDHA loss of function mutations in a subset of young adult wild-type gastrointestinal stromal tumors
    Antoine Italiano
    Departments of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    BMC Cancer 12:408. 2012
    ..A recent report identified four non-syndromic, KIT/PDGFRA WT GIST harboring compound heterozygous or homozygous mutations in SDHA encoding the main subunit of the SDH complex II.
  58. doi Prokaryotic assembly factors for the attachment of flavin to complex II
    Matthew B McNeil
    Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand
    Biochim Biophys Acta 1827:637-47. 2013
    ..Complex II consists of four subunits including a catalytic flavoprotein (SdhA), an iron-sulphur subunit (SdhB) and two hydrophobic membrane anchors (SdhC and SdhD)...
  59. ncbi [Mitochondrial respiratory chain complex Ⅱ deficiency and diseases]
    Yan Yan Ma
    Department of Pediatrics, Peking University First Hospital, Beijing 100034, China
    Zhongguo Dang Dai Er Ke Za Zhi 14:723-7. 2012
    ..Complex Ⅱ activities decreased in affected tissues. Pathogenic mutations in SDHA gene and SDHAF1 gene encoding assembly factor have been found so far...
  60. doi Succinate dehydrogenase deficiency is associated with decreased 5-hydroxymethylcytosine production in gastrointestinal stromal tumors: implications for mechanisms of tumorigenesis
    Emily F Mason
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Mod Pathol 26:1492-7. 2013
    ..were examined via immunohistochemistry in a cohort of 30 genotyped GISTs, including 10 SDH-deficient tumors (5 SDHA mutant; 1 SDHB mutant; 1 SDHC mutant; 3 unknown), 14 tumors with KIT mutations (10 in exon 11; 3 in exon 9; 1 in ..
  61. pmc Next-generation sequencing in the clinical genetic screening of patients with pheochromocytoma and paraganglioma
    Joakim Crona
    Department of Surgical Sciences Uppsala University S 751 85, Uppsala Sweden
    Endocr Connect 2:104-11. 2013
    ..PGLs) are caused by germline or somatic mutations in one of the 11 hitherto known susceptibility genes: SDHA, SDHB, SDHC, SDHD, SDHAF2, VHL, HIF2A (EPAS1), RET, NF1, TMEM127 and MAX...
  62. doi Expression stability of reference genes for quantitative RT-PCR of healthy and diseased pituitary tissue samples varies between humans, mice, and dogs
    Sarah J van Rijn
    Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, PO Box 80154, 3508 TD, Utrecht, The Netherlands
    Mol Neurobiol 49:893-9. 2014
    ..hydroxymethylbilane synthase (hmbs), beta-2-microglobulin (b2m), succinate dehydrogenase complex subunit A (sdha), and glyceraldehyde 3 phosphate dehydrogenase 1 (gapdh), was studied in pituitary tissue (normal and adenoma) from ..
  63. doi Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA
    Ayenachew Bezawork-Geleta
    2Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia K N T
    FASEB J 28:1794-804. 2014
    ..Although SDH5(G78R) is deficient in its ability to promote SDHA flavinylation, it has remained unclear whether impairment to its import, structure, or stability contributes to its ..
  64. pmc Radiation-induced alterations in mitochondria of the rat heart
    Vijayalakshmi Sridharan
    a University of Arkansas for Medical Sciences, Department of Pharmaceutical Sciences, Division of Radiation Health, Little Rock, Arkansas
    Radiat Res 181:324-34. 2014
    ..PTEN induced putative kinase-1 (PINK-1); mitochondrial respiration and protein levels of succinate dehydrogenase A (SDHA); and the 70 kDa subunit of complex II...
  65. doi Rare germline mutations identified by targeted next-generation sequencing of susceptibility genes in pheochromocytoma and paraganglioma
    Jenny Welander
    Department of Clinical and Experimental Medicine J W, O G, P S, Faculty of Health Sciences, Linkoping University, Departments of Surgery O G and Clinical Genetics P S, County Council of Ostergotland, Linköping SE 58185, Sweden Department of Oncology Pathology A A, C C J, A H, C L, Karolinska Institutet, Cancer Center Karolinska A A, C C J, A H, C L, Karolinska University Hospital Solna, and Department of Molecular Medicine and Surgery A A, C C J, M B, C L, Karolinska Institutet, Karolinska University Hospital, Stockholm SE 17176, Sweden and Wisconsin National Primate Research Center R W W, University of Wisconsin Madison, Madison, Wisconsin 53715
    J Clin Endocrinol Metab 99:E1352-60. 2014
    ..Pheochromocytomas and paragangliomas have a highly diverse genetic background, with a third of the cases carrying a germline mutation in 1 of 14 identified genes...
  66. doi Exploring the association of succinate dehydrogenase complex mutations with lymphoid malignancies
    R Renella
    Department of Pediatric Oncology, Dana Farber Cancer Institute, 450 Brookline Ave, Boston, MA, USA
    Fam Cancer 13:507-11. 2014
    ..SDH) complex exerts a fundamental role in mitochondrial cellular respiration and mutations in its encoding genes (SDHA, SDHB, SDHC, SDHD, collectively referred to as SDHx) lead to a number of inherited endocrine cancer predisposition ..
  67. doi Expression stability of common housekeeping genes is differently affected by bowel inflammation and cancer: implications for finding suitable normalizers for inflammatory bowel disease studies
    Malgorzata Krzystek-Korpacka
    Departments of Medical Biochemistry, and Gastrointestinal and General Surgery, Wroclaw Medical University, Wroclaw, Poland Department of Food Hygiene and Consumer Health, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland and Wroclaw Research Center EIT, Wroclaw, Poland
    Inflamm Bowel Dis 20:1147-56. 2014
    ..Expression stability of 15 HKG (ACTB, B2M, GAPDH, GUSB, HPRT1, IPO8, MRPL19, PGK1, PPIA, RPLP0, RPS23, SDHA, TBP, UBC, and YWHAZ) in 166 bowel specimens (91 normal, 35 cancerous, and 40 inflamed) was ranked by coefficients ..
  68. pmc SDHAF4 promotes mitochondrial succinate dehydrogenase activity and prevents neurodegeneration
    Jonathan G Van Vranken
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Cell Metab 20:241-52. 2014
    ..specifically with the catalytic Sdh1 subunit in the mitochondrial matrix, facilitating its association with Sdh2 and the subsequent assembly of the SDH holocomplex...
  69. doi Targeting mitochondria with methylene blue protects mice against acetaminophen-induced liver injury
    Kang Kwang Lee
    Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT
    Hepatology 61:326-36. 2015
    ..APAP treatment inhibited complex II activity ex vivo, but did not alter the protein expression levels of subunits SdhA or SdhC after 4 hours.
  70. doi Selection of appropriate reference genes for RT-qPCR analysis in Berkshire, Duroc, Landrace, and Yorkshire pigs
    Sang Je Park
    National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363 883, Republic of Korea Electronic address
    Gene 558:152-8. 2015
    ..of 15 well-known candidate reference genes (ACTB, ALDOA, B2M, GAPDH, HPAR1, HSPCB, PGK1, POLR2G, PPIA, RPL4, RPS18, SDHA, TBP, TOP2B, and YWHAZ) in seven body tissues (liver, lung, kidney, spleen, stomach, small intestine, and large ..
  71. doi The Flower Tea Coreopsis tinctoria Increases Insulin Sensitivity and Regulates Hepatic Metabolism in Rats Fed a High-Fat Diet
    Baoping Jiang
    Institute of Medicinal Plant Development B J, L L, W W, W Z, K H, L X, P X, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100194, China and Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine B J, W W, L X, P X, Ministry of Education, Beijing 100194, China
    Endocrinology 156:2006-18. 2015
    ..Liquid chromatography-mass spectrometry-based metabolomics approaches are helpful to further understand diabetes-related mechanisms. ..
  72. pmc Urinary Bladder Paragangliomas: How Immunohistochemistry Can Assist to Identify Patients With SDHB Germline and Somatic Mutations
    Alessio Giubellino
    Translational Surgical Pathology section, Laboratory of Pathology, National Cancer Institute Eunice Kennedy Shriver, National Institute of Child Health and Human Development Urologic Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD
    Am J Surg Pathol 39:1488-92. 2015
    ..They were found to be either sporadic tumors or part of von Hippel-Lindau syndrome. Staining for SDHA was positive in all cases...
  73. doi Renal carcinoma associated with a novel succinate dehydrogenase A mutation: a case report and review of literature of a rare subtype of renal carcinoma
    Yasemin Ozluk
    Department of Pathology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey
    Hum Pathol 46:1951-5. 2015
    Renal cell carcinoma (RCC) linked to germline mutation of succinate dehydrogenase subunits A, B, C, and D (SDHA, SDHB, SDHC, and SDHD, respectively) has been recently included as a provisional entity in the 2013 International Society of ..
  74. pmc Cardiac-specific succinate dehydrogenase deficiency in Barth syndrome
    Jan Dudek
    Department of Cellular Biochemistry, University Medical Center Gottingen, Gottingen, Germany
    EMBO Mol Med 8:139-54. 2016
    ..As a similar defect of SDH is apparent in patient cell-derived cardiomyocytes, we conclude that these defects represent a molecular basis for the cardiac pathology in Barth syndrome. ..
  75. doi [Succinate dehydrogenase (SDH)-deficient renal cell carcinoma]
    A Agaimy
    Pathologisches Institut, Friedrich Alexander Universitat Erlangen Nurnberg, Universitatsklinikum Erlangen, Krankenhausstrasse 8 10, 91054, Erlangen, Deutschland
    Pathologe 37:144-52. 2016
    ..The complex is composed of four major subunits, SDHA, SDHB, SDHC and SDHD...
  76. pmc Reference Genes for Addressing Gene Expression of Bladder Cancer Cell Models under Hypoxia: A Step Towards Transcriptomic Studies
    Luis Lima
    Experimental Pathology and Therapeutics Group Research Center, Portuguese Institute of Oncology of Porto IPO Porto, 4200 072, Porto, Portugal
    PLoS ONE 11:e0166120. 2016
    ..and experimental conditions out of a panel of seven putative candidates (HPRT, ACTB, 18S, GAPDH, TBP, B2M, and SDHA)...
  77. ncbi Induction of apoptosis in cells expressing exogenous Hippi, a molecular partner of huntingtin-interacting protein Hip1
    Pritha Majumder
    Structural Genomics Section, Saha Institute of Nuclear Physics, 1 AF Bidhan Nagar, Kolkata 700 064, India
    Neurobiol Dis 22:242-56. 2006
    ..Besides, the expression SDHA and SDHB, nuclear genes, subunits of mitochondrial complex II were decreased in GFP-Hippi expressing cells...
  78. doi Succinate dehydrogenase gene arrangement and expression in Anaplasma phagocytophilum
    Robert F Massung
    Rickettsial Zoonoses Branch, CDC, Atlanta, GA 30333, USA
    Gene 414:41-8. 2008
    ..The recently completed sequence of the A. phagocytophilum genome confirmed our findings and indicated that the sdhA and sdhB genes are duplicated in a tandem orientation, and located distant from the sdhC and sdhD genes...
  79. pmc The Hfq-dependent small noncoding RNA NrrF directly mediates Fur-dependent positive regulation of succinate dehydrogenase in Neisseria meningitidis
    Matteo M E Metruccio
    Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    J Bacteriol 191:1330-42. 2009
    ..Recently, a Fur-regulated sRNA, NrrF, was identified that is involved in the iron regulation of the sdhA and sdhC succinate dehydrogenase genes...
  80. pmc Inhibition of α-KG-dependent histone and DNA demethylases by fumarate and succinate that are accumulated in mutations of FH and SDH tumor suppressors
    Mengtao Xiao
    Molecular and Cell Biology Laboratory, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
    Genes Dev 26:1326-38. 2012
    ..These epigenetic alterations associated with mutations of FH and SDH likely contribute to tumorigenesis...
  81. doi Yeast model for evaluating the pathogenic significance of SDHB, SDHC and SDHD mutations in PHEO-PGL syndrome
    Elena Panizza
    Department of Life Sciences, University of Parma, Parma 43124, Italy
    Hum Mol Genet 22:804-15. 2013
    ..Heterozygous germline mutations in SDHA, SDHB, SDHC, SDHD and in the assembly factor encoding gene SDHAF2 have all been shown to predispose to heritable ..
  82. doi 123 changes in expression of genes associated with genetic variation in pre-implantation development of the bovine embryo
    M S Ortega
    Department of Animal Sciences, University of Florida, Gainesville, FL, USA
    Reprod Fertil Dev 26:175. 2013
    ..The RNA was purified and synthesised into cDNA for real-time qPCR analysis. The YWHAZ, GAPDH, and SDHA were used as steady-state controls of expression. A total of 5 pools were analysed for each of the 6 stages...
  83. doi Expression of metabolism-related proteins in invasive lobular carcinoma: comparison to invasive ductal carcinoma
    Yon Hee Kim
    Department of Pathology, Severance Hospital, Yonsei University College of Medicine, 50 Yonsei ro, Seodaemun gu, Seoul, 120 752, South Korea
    Tumour Biol 35:10381-93. 2014
    ..glycolysis (Glut-1, hexokinase II, CAIX, MCT4), glutaminolysis (GLS1, GDH, ASCT2), mitochondria (ATP synthase, SDHA, SDHB), and serine/glycine metabolism (PHGDH, PSAT1, PSPH, SHMT1, GLDC) related proteins...
  84. doi The validation of housekeeping genes as a reference in quantitative Real Time PCR analysis: application in the milk somatic cells and frozen whole blood of goats infected with caprine arthritis encephalitis virus
    Justyna Jarczak
    Department of Animal Sciences, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec Postępu 36A, 05 552 Magdalenka, Poland Electronic address
    Gene 549:280-5. 2014
    ..RPLP0) was determined in milk somatic cells, while ACTB, PPIA, RPLP0, succinate dehydrogenase complex subunit A (SDHA), zeta polypeptide (YWHAZ), battenin (CLN3), eukaryotic translation initiation factor 3K (EIF3K) and TATA box-..
  85. doi Caffeic acid attenuates rat liver reperfusion injury through sirtuin 3-dependent regulation of mitochondrial respiratory chain
    Hong Na Mu
    Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China
    Free Radic Biol Med 85:237-49. 2015
    ..CA attenuated rat liver microcirculatory disturbance and oxidative injury through regulation of Sirt3 and the mitochondrial respiratory chain...
  86. pmc Identification of reliable reference genes for qRT-PCR studies of the developing mouse mammary gland
    Anoeska Agatha Alida van de Moosdijk
    Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
    Sci Rep 6:35595. 2016
    ..Clock, Ctbp1, Phf7, Prdx1, Sugp2, Taf11 and Usp7) to eight traditional ones (18S, Actb, Gapdh, Hmbs, Hprt, Rpl13a, Sdha and Tbp) and analysed all genes for stable expression in the mouse mammary gland from pre-puberty to adulthood ..
  87. ncbi Characterisation of Phaseolus symbionts isolated from Mediterranean soils and analysis of genetic factors related to pH tolerance
    U B Priefer
    RWTH Aachen, Okologie des Bodens, Worringer Weg 1, 52056, Aachen, Germany
    J Biotechnol 91:223-36. 2001
    ..low pH tolerance were found to be mutated in genes related to cobalmin biosynthesis and in succinate dehydrogenase (sdhA)...
  88. ncbi Hereditary paraganglioma/pheochromocytoma and inherited succinate dehydrogenase deficiency
    Judith Favier
    Département de génétique, Hopital Europeen Georges Pompidou, Assistance Publique Hopitaux de Paris, Université Paris V and INSERM U36, College de France, France
    Horm Res 63:171-9. 2005
    ..Germline succinate dehydrogenase subunit A (SDHA) mutations have been reported in a few patients with a classical mitochondrial neurodegenerative disease...
  89. ncbi Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization?
    Falk Ohl
    Department of Urology, Charite Universitatsmedizin Berlin, Campus Mitte, Schumannstrasse 20 21, 10098 Berlin, Germany
    J Mol Med (Berl) 83:1014-24. 2005
    ..Expression of mRNA encoding ACTB, ALAS1, ALB, B2M, G6PD, GAPD, HMBS, HPRT1, K-ALPHA-1, POLR2A, PPIA, RPL13A, SDHA, TBP, UBC, and YWHAZ was examined in matched, microdissected malignant and nonmalignant tissue specimens obtained ..
  90. ncbi Technical note: validation of internal control genes for gene expression analysis in bovine polymorphonuclear leukocytes
    A De Ketelaere
    Department of Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    J Dairy Sci 89:4066-9. 2006
    ..In this study, 8 commonly used reference genes (ACT, GAPD, H2A, TBP, HPRT1, SDHA, YWHAZ, and 18S rRNA) were evaluated in bovine polymorphonuclear leukocytes...
  91. doi Calcium signalling-dependent mitochondrial dysfunction and bioenergetics regulation in respiratory chain Complex II deficiency
    E Mbaya
    INSERM U 807, Paris, F 75015 France
    Cell Death Differ 17:1855-66. 2010
    ..These results revealed the primary function for Ca(2+) signalling in the control of mitochondrial dysfunction and cellular bioenergetics outcomes linked to respiratory chain Complex II deficiency...
  92. doi Genetics and molecular pathogenesis of pheochromocytoma and paraganglioma
    S R Galan
    Division of Endocrinology and Diabetology, Faculty of Medicine, Philipp s University Marburg, University Hospital Giessen and Marburg, Marburg, Germany
    Clin Endocrinol (Oxf) 78:165-75. 2013
    ..gene (NF1), genes encoding the succinate dehydrogenase (SDH) complex subunits SDHB, SDHC, and SDHD, but also SDHA, the gene encoding the enzyme responsible for the flavination of SDHA (SDHAF2 or hSDH5), and the newly described ..
  93. doi Reference gene for primary culture of prostate cancer cells
    Aline Francielle Damo Souza
    Laboratório de Biologia Molecular Endócrina e Tumoral, Departamento de Fisiologia, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, 2º andar, laboratório 09, Porto Alegre, RS 90050 170, Brazil
    Mol Biol Rep 40:2955-62. 2013
    ..hypoxanthine phosphoribosyltransferase 1 (HPRT1); succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA); TATA box binding protein (TBP); ubiquitin C (UBC); tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation ..
  94. pmc Immunohistochemical loss of succinate dehydrogenase subunit A (SDHA) in gastrointestinal stromal tumors (GISTs) signals SDHA germline mutation
    Markku Miettinen
    Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892, USA
    Am J Surg Pathol 37:234-40. 2013
    ..More recently, germline mutations in SDH subunit A gene (SDHA) have also been reported in few patients with KIT/PDGFRA wild-type GISTs...
  95. pmc Identification of cell-specific patterns of reference gene stability in quantitative reverse-transcriptase polymerase chain reaction studies of embryonic, placental and neural stem models of prenatal ethanol exposure
    Mindy N Carnahan
    College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
    Alcohol 47:109-20. 2013
    ..This list included: Actb, B2m, Gapdh, Gusb, H2afz, Hk2, Hmbs, Hprt, Mrpl1, Pgk1, Ppia, Sdha, Tbp, and Ywhaz...
  96. pmc Malate and fumarate extend lifespan in Caenorhabditis elegans
    Clare B Edwards
    Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida Tampa, Florida, USA
    PLoS ONE 8:e58345. 2013
    ..malate addition did not occur in fumarase (fum-1), glyoxylate shunt (gei-7), succinate dehydrogenase flavoprotein (sdha-2), or soluble fumarate reductase F48E8.3 RNAi knockdown worms...
  97. pmc Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli
    M G Darlison
    Biochem J 223:507-17. 1984
    ..It is separated by a 15 base-pair intergenic region from the preceding flavoprotein gene (sdhA) and is the distal gene of an operon that also includes genes (sdhC and D) encoding two hydrophobic subunits, ..
  98. doi Loss of expression of SDHA predicts SDHA mutations in gastrointestinal stromal tumors
    Andrew J Wagner
    Dana Farber Cancer Institute, Center for Sarcoma and Bone Oncology, Harvard Medical School, Boston, MA, USA
    Mod Pathol 26:289-94. 2013
    ..Because of the complexity of its locus (15 exons) and the presence of three pseudogenes, SDHA is rarely analyzed...
  99. doi Integrative genomics reveals frequent somatic NF1 mutations in sporadic pheochromocytomas
    Jenny Welander
    Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden
    Hum Mol Genet 21:5406-16. 2012
    ..of copy number alterations, gene expression, promoter methylation and somatic mutations in the genes RET, VHL, NF1, SDHA, SDHB, SDHC, SDHD, SDHAF2, KIF1Bβ, TMEM127 and MAX, which have been associated with hereditary pheochromocytoma or ..
  100. doi Expression of reference genes and T helper 17 associated cytokine genes in the equine intestinal tract
    Bernt Hjertner
    Section of Immunology, Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P O Box 588, SE 751 23 Uppsala, Sweden Electronic address
    Vet J 197:817-23. 2013
    ..phosphoribosyltransferase (HPRT), 60S ribosomal protein L32 (RPL32), succinate dehydrogenase complex subunit A (SDHA) and transferrin receptor 1 protein coding (TFRC)in the equine intestine was evaluated by quantitative PCR...

Research Grants13

  1. MOLEC GENETICS OF COMPLEX I IN MAMMALIAN MITOCHONDRIA
    Immo Scheffler; Fiscal Year: 2004
    ..Such mice promise to become model systems for mitochondrial diseases. 5) Known cDNAs will be tested to identify the defective gene in the other four complementation groups with complex I defects. ..
  2. Using Synthetic Lethality to Select Cancer Drug Targets
    William Kaelin; Fiscal Year: 2005
    ..Moreover, they may establish a new paradigm for the selection of anticancer drug targets based on inactivating mutations of TSG. ..
  3. 2006 Cancer Model Mechanisms
    William Kaelin; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  4. Regulation of Streptococcus pyogenes exoproteins
    MICHAEL CHAUSSEE; Fiscal Year: 2007
    ..Specific Aim 4. Determine if Rgg binds to promoter regions of genes encoding exoproteins to control expression. Electrophoretic mobility-shift assays will be used to determine if Rgg binds to the promoter regions of mf-1 and speB. ..
  5. O2 Sensing in Hypoxic Pulmonary Vasoconstriction
    Paul Schumacker; Fiscal Year: 2009
    ..Collectively, these studies will test whether a common O2 sensing mechanism functions in PA myocytes and endothelial cells to trigger their diverse responses in HPV. ..
  6. Proximal determinants of risk for tularemia outbreaks
    Sam Telford; Fiscal Year: 2004
    ..tularensis in the environment; and, ultimately, a better understanding of F. tularensis-host interactions. ..
  7. Perpetuation of Francisella tularensis
    Sam R Telford; Fiscal Year: 2010
    ..Taken together, these observations will provide a basis for understanding how the agent of tularemia is perpetuated on Martha's Vineyard, and why pneumonic human disease is prevalent there. ..
  8. OXYGEN SENSING AND CELL SIGNALING IN HYPOXIA
    Paul Schumacker; Fiscal Year: 2001
    ..These studies will identify a novel pathway of cellular O2 detection, and may help clarify understanding of how cells adapt to lowered O2 conditions. ..
  9. O2 SENSING BY MITOCHONDRIA DURING INTERMITTENT HYPOXIA
    Paul Schumacker; Fiscal Year: 2003
    ..Collectively, these studies could identify a novel mechanism of O2 sensing in the lung, and provide a mechanistic explanation for the activation of gene transcritpion and cellular proliferation during intermittent hypoxia. ..
  10. Real-Time Imaging of Hypoxia based on VHL Activity
    William Kaelin; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  11. Lung Injury Protection by Coagulation Blockade
    CLAUDE PIANTADOSI; Fiscal Year: 2008
    ..A successfully optimized strategy could greatly attenuate persistence of pulmonary inflammation and facilitate the resolution of human ARDS. ..
  12. A Luciferase Fusion Protein Library to Identify & Monitor Ubiquitylation Targets
    William Kaelin; Fiscal Year: 2008
    ..Reisolation of HIF in aims 2 and 3 would constitute proof of concept with respect to the potential utility of this approach. ..
  13. Nitric oxide and mitochondrial biogenesis in sepsis
    Claude A Piantadosi; Fiscal Year: 2010
    ..abstract_text> ..