ROCK1

Summary

Gene Symbol: ROCK1
Description: Rho associated coiled-coil containing protein kinase 1
Alias: P160ROCK, ROCK-I, rho-associated protein kinase 1, p160 ROCK-1, renal carcinoma antigen NY-REN-35
Species: human

Top Publications

  1. ncbi Rocks: multifunctional kinases in cell behaviour
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
    Nat Rev Mol Cell Biol 4:446-56. 2003
  2. pmc Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor
    Fabrice Le Boeuf
    Le Centre de Recherche en Cancérologie de l Université Laval, Quebec, Quebec G1R 2J6, Canada
    Mol Biol Cell 17:3508-20. 2006
  3. doi MicroRNA-340 suppresses osteosarcoma tumor growth and metastasis by directly targeting ROCK1
    Xin Zhou
    Department of Orthopaedic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, China
    Biochem Biophys Res Commun 437:653-8. 2013
  4. doi HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9
    Ruifen Xu
    Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi an Jiaotong University, Xi an, China
    Brain Res 1436:13-9. 2012
  5. ncbi Roles of Rho guanosine 5'-triphosphatase A, Rho kinases, and extracellular signal regulated kinase (1/2) in prostaglandin E2-mediated migration of first-trimester human extravillous trophoblast
    Catalin Nicola
    Department of Anatomy, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Endocrinology 149:1243-51. 2008
  6. pmc Rho kinase-1 mediates cardiac fibrosis by regulating fibroblast precursor cell differentiation
    Sandra B Haudek
    DeBakey Heart Center, Baylor College of Medicine and The Methodist Hospital, One Baylor Plaza F620, Houston TX 77030, USA
    Cardiovasc Res 83:511-8. 2009
  7. pmc Rho kinase regulates the survival and transformation of cells bearing oncogenic forms of KIT, FLT3, and BCR-ABL
    Raghuveer Singh Mali
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, USA
    Cancer Cell 20:357-69. 2011
  8. ncbi Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics
    D C Edwards
    Department of Chemistry, University of California at San Diego, La Jolla 92093 0650, USA
    Nat Cell Biol 1:253-9. 1999
  9. pmc Rho-kinase mediates hyperglycemia-induced plasminogen activator inhibitor-1 expression in vascular endothelial cells
    Yoshiyuki Rikitake
    Vascular Medicine Research Unit, Brigham and Women s Hospital and Harvard Medical School, Boston, Mass, USA
    Circulation 111:3261-8. 2005
  10. ncbi Prostate-derived sterile 20-like kinase 2 (PSK2) regulates apoptotic morphology via C-Jun N-terminal kinase and Rho kinase-1
    Ceniz Zihni
    KCL School of Medicine and Dentistry, Rayne Institute, Kings College London, 123 Coldharbour Lane, London SE5 9NU, UK
    J Biol Chem 281:7317-23. 2006

Research Grants

  1. Rho Kinase and Airway Hyperresponsiveness
    Stephanie A Shore; Fiscal Year: 2013
  2. MATRIX MEDIATORS OF WOUND HEALING
    Paul J Higgins; Fiscal Year: 2010
  3. Young Bum Kim; Fiscal Year: 2016
  4. JAMES KUANG JAN LIAO; Fiscal Year: 2014
  5. JAMES KUANG JAN LIAO; Fiscal Year: 2014
  6. ROCK inhibitor suppression of GVHD with retention of GVL response
    Sujatha Iyengar; Fiscal Year: 2012
  7. Lei Wei; Fiscal Year: 2016
  8. Investigating the RhoA/ROCK pathway for the treatment of Alzheimer's disease
    JEREMY HARTFORD HERSKOWITZ; Fiscal Year: 2013
  9. THOMAS PATRICK LOUGHRAN; Fiscal Year: 2016
  10. Qun Lu; Fiscal Year: 2014

Detail Information

Publications209 found, 100 shown here

  1. ncbi Rocks: multifunctional kinases in cell behaviour
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
    Nat Rev Mol Cell Biol 4:446-56. 2003
    ..Recent experiments have defined new functions of ROCKs in cells, including centrosome positioning and cell-size regulation, which might contribute to various physiological and pathological states...
  2. pmc Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor
    Fabrice Le Boeuf
    Le Centre de Recherche en Cancérologie de l Université Laval, Quebec, Quebec G1R 2J6, Canada
    Mol Biol Cell 17:3508-20. 2006
    ..Then, activated Pyk2 triggers the phosphorylation of FAK on Tyr407, promoting cell migration...
  3. doi MicroRNA-340 suppresses osteosarcoma tumor growth and metastasis by directly targeting ROCK1
    Xin Zhou
    Department of Orthopaedic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, China
    Biochem Biophys Res Commun 437:653-8. 2013
    ..b>ROCK1 was identified as a target of miR-340, and ectopic expression of miR-340 downregulated ROCK1 by direct binding to ..
  4. doi HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9
    Ruifen Xu
    Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi an Jiaotong University, Xi an, China
    Brain Res 1436:13-9. 2012
    ..Taken together, these results show that the HIV-1 Tat protein disrupts BBB integrity, at least in part by decreasing the production of occludin...
  5. ncbi Roles of Rho guanosine 5'-triphosphatase A, Rho kinases, and extracellular signal regulated kinase (1/2) in prostaglandin E2-mediated migration of first-trimester human extravillous trophoblast
    Catalin Nicola
    Department of Anatomy, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Endocrinology 149:1243-51. 2008
    ..These results suggest that ROCK and ERK1/2 play more important roles than RhoA in PGE(2)-mediated migration stimulation of first-trimester trophoblasts...
  6. pmc Rho kinase-1 mediates cardiac fibrosis by regulating fibroblast precursor cell differentiation
    Sandra B Haudek
    DeBakey Heart Center, Baylor College of Medicine and The Methodist Hospital, One Baylor Plaza F620, Houston TX 77030, USA
    Cardiovasc Res 83:511-8. 2009
    ..Because Rho-associated kinase-1 (ROCK-1) has been implicated in fibrosis and leukocyte TEM, we investigated its involvement in I/RC...
  7. pmc Rho kinase regulates the survival and transformation of cells bearing oncogenic forms of KIT, FLT3, and BCR-ABL
    Raghuveer Singh Mali
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, USA
    Cancer Cell 20:357-69. 2011
    ..In summary we describe a pathway involving PI3K/Rho/ROCK/MLC that may contribute to myeloproliferative disease and/or acute myeloid leukemia in humans...
  8. ncbi Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics
    D C Edwards
    Department of Chemistry, University of California at San Diego, La Jolla 92093 0650, USA
    Nat Cell Biol 1:253-9. 1999
    ..A Pak1-specific inhibitor, corresponding to the Pak1 autoinhibitory domain, blocks LIM-kinase-induced cytoskeletal changes. Activated GTPases can thus regulate actin depolymerization through Pak1 and LIM-kinase...
  9. pmc Rho-kinase mediates hyperglycemia-induced plasminogen activator inhibitor-1 expression in vascular endothelial cells
    Yoshiyuki Rikitake
    Vascular Medicine Research Unit, Brigham and Women s Hospital and Harvard Medical School, Boston, Mass, USA
    Circulation 111:3261-8. 2005
    ..The induction of PAI-1 expression by hyperglycemia involves oxidative stress and protein kinase C (PKC). However, the mechanism by which hyperglycemia increases PAI-1 expression is unknown...
  10. ncbi Prostate-derived sterile 20-like kinase 2 (PSK2) regulates apoptotic morphology via C-Jun N-terminal kinase and Rho kinase-1
    Ceniz Zihni
    KCL School of Medicine and Dentistry, Rayne Institute, Kings College London, 123 Coldharbour Lane, London SE5 9NU, UK
    J Biol Chem 281:7317-23. 2006
    ..Our findings suggest that PSKs form a subgroup of sterile 20 (STE20)-like kinases that regulate different cytoskeletal processes...
  11. pmc Opposite effects of HIV-1 p17 variants on PTEN activation and cell growth in B cells
    Cinzia Giagulli
    Department of Experimental and Applied Medicine, University of Brescia, Brescia, Italy
    PLoS ONE 6:e17831. 2011
    ..Moreover, it provides the first evidence on the presence of a p17 natural variant mimicking the p17Δ36-induced signalling in B cells and displaying the capacity of promoting B cell growth and tumorigenesis...
  12. pmc Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin
    Andrew L Smith
    Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232, USA
    Mol Biol Cell 23:99-110. 2012
    ..or ReCLIP, we previously identified a physical interaction between p120 and Rho-associated protein kinase 1 (ROCK1), a major effector of RhoA...
  13. pmc The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase
    T Ishizaki
    Department of Pharmacology, Kyoto University Faculty of Medicine, Japan
    EMBO J 15:1885-93. 1996
    ..When co-expressed with RhoA in COS cells, p160 was co-precipitated with the expressed Rho and its kinase activity was activated, indicating that p160 can associate physically and functionally with Rho both in vitro and in vivo...
  14. ncbi Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progressio
    Lilly Y W Bourguignon
    Department of Medicine, University of California at San Francisco and the Endocrine Unit 111N, Veterans Affairs Medical Center, San Francisco, Calfornia 94121, USA
    J Biol Chem 278:29420-34. 2003
    ..PI 3-kinase membrane localization, AKT signaling, and cytokine (M-CSF) production during HA-mediated breast cancer progression...
  15. ncbi Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase
    J Feng
    First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514 8507, Japan
    J Biol Chem 274:37385-90. 1999
    ..In summary, these results offer strong support for a physiological role of Rho-kinase in regulation of MP activity...
  16. ncbi Phosphorylation of collapsin response mediator protein-2 by Rho-kinase. Evidence for two separate signaling pathways for growth cone collapse
    N Arimura
    Division of Signal Transduction, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma 630 0101, Japan
    J Biol Chem 275:23973-80. 2000
    ..These results demonstrate the existence of Rho-kinase-dependent and -independent pathways for growth cone collapse and suggest that CRMP-2 phosphorylation by Rho-kinase is involved in the former pathway...
  17. doi Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion
    Shuh Narumiya
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, 606 8501, Japan
    Cancer Metastasis Rev 28:65-76. 2009
    ..Thus, Rho mediates various phenotypes of malignant transformation by Ras and Src through its effectors, ROCK and mDia...
  18. doi Activating ROCK1 somatic mutations in human cancer
    P A Lochhead
    The Beatson Institute for Cancer Research, Glasgow, Scotland G61 1BD, UK
    Oncogene 29:2591-8. 2010
    ..b>ROCK1 is an essential effector kinase downstream of Rho GTPases, an important pathway involved in cell migration...
  19. ncbi Phosphorylation of glial fibrillary acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase
    H Kosako
    Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi 464, Japan
    J Biol Chem 272:10333-6. 1997
    ..Furthermore, the phosphorylation of GFAP by Rho-kinase results in a nearly complete inhibition of its filament formation in vitro. The possibility that Rho-kinase is a candidate for cleavage furrow kinase is discussed...
  20. doi Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection
    Tamia K Lapointe
    Department of Biological Sciences and Inflammation Research Network, University of Calgary, Alberta, Canada
    Cell Microbiol 12:692-703. 2010
    ..pylori disrupts gastric epithelial barrier structure via IL-1RI-dependent activation of ROCK, which in turn mediates tight junctional claudin-4 disruption...
  21. ncbi Balance between activities of Rho kinase and type 1 protein phosphatase modulates turnover of phosphorylation and dynamics of desmin/vimentin filaments
    H Inada
    Division of Biochemistry, Aichi Cancer Center Research Institute, 1 1 Chikusa ku, Nagoya, Aichi 464 8681, Japan
    J Biol Chem 274:34932-9. 1999
    ....
  22. pmc Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes
    Olga Barreiro
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 28006 Madrid, Spain
    J Cell Biol 157:1233-45. 2002
    ..These results provide the first characterization of an endothelial docking structure that plays a key role in the firm adhesion of leukocytes to the endothelium during inflammation...
  23. pmc Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis
    Jiang Chang
    Center for Molecular Development and Disease, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, 2121 West Holcombe Boulevard, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:14495-500. 2006
    ..These data suggest an obligatory role for ROCK-1 cleavage in promoting apoptotic signals in myocardial hypertrophy and/or failure...
  24. doi Phosphorylated caveolin-1 regulates Rho/ROCK-dependent focal adhesion dynamics and tumor cell migration and invasion
    Bharat Joshi
    Department of Cellular and Physiological Sciences, Life Sciences Institute, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    Cancer Res 68:8210-20. 2008
    ....
  25. ncbi Identification of the Rho-binding domain of p160ROCK, a Rho-associated coiled-coil containing protein kinase
    K Fujisawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 271:23022-8. 1996
    ..Furuyashiki, T., Ishizaki, T., Watanabe, G., Watanabe, N., Fujisawa, K., Morii, N., Madaule, P., and Narumiya, S. (1996) J. Biol. Chem. 271, 13556-13560) and may define a distinct class of Rho effector motif...
  26. pmc Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
    T Matsui
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 140:647-57. 1998
    ....
  27. ncbi Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis
    H Goto
    Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Aichi 464, Japan
    J Biol Chem 273:11728-36. 1998
    ..All these accumulating observations increase the possibility that Rho-kinase may have a definite role in governing regulatory processes in assembly-disassembly and turnover of vimentin filaments at the cleavage furrow during cytokinesis...
  28. ncbi Phosphorylation of moesin by rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures
    N Oshiro
    Division of Signal Transduction, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma 630 0101, Japan
    J Biol Chem 273:34663-6. 1998
    ..These results indicate that Rho-kinase regulates moesin phosphorylation downstream of Rho in vivo and that the phosphorylation of moesin by Rho-kinase plays a crucial role in the formation of microvilli-like structures...
  29. pmc Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility
    Y Fukata
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 0101, Japan
    J Cell Biol 145:347-61. 1999
    ....
  30. ncbi Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
    M Maekawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8315, Japan
    Science 285:895-8. 1999
    ..These results indicate that phosphorylation of LIM-kinase by ROCK and consequently increased phosphorylation of cofilin by LIM-kinase contribute to Rho-induced reorganization of the actin cytoskeleton...
  31. pmc Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo
    Y Kawano
    Division of Signal Transduction, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma 630 0101, Japan
    J Cell Biol 147:1023-38. 1999
    ....
  32. ncbi Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop
    K Ohashi
    Biological Institute, Graduate School of Science, Tohoku University, Sendai 980 8578, Japan
    J Biol Chem 275:3577-82. 2000
    ....
  33. ncbi Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase
    T Sumi
    Division of Biochemistry, Department of Oncology, Biomedical Research Center, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 276:670-6. 2001
    ..Together with the finding that LIMK1 is regulated by Pak1, LIMK1 and LIMK2 are regulated by different protein kinases downstream of distinct Rho family GTPases...
  34. ncbi Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing
    M Sebbagh
    INSERM U461, Faculte de Pharmacie, 92296 Chatenay Malabry, France
    Nat Cell Biol 3:346-52. 2001
    ..Here we identify ROCK I, an effector of the small GTPase Rho, as a new substrate for caspases...
  35. ncbi Expression of ROCK-1 in human pancreatic cancer: its down-regulation by morpholino oligo antisense can reduce the migration of pancreatic cancer cells in vitro
    Kenzo Kaneko
    Department of Gastroenterology, Division of Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan
    Pancreas 24:251-7. 2002
    ..Results of previous studies showed that the elevated activity of ROCK-1, one of the isomers of ROCK kinases, led to an increase in the activity of invasion and metastasis of cancer cell lines...
  36. ncbi ROCK and Dia have opposing effects on adherens junctions downstream of Rho
    Erik Sahai
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 4:408-15. 2002
    ..The ability of Dia1 to regulate the actin network is crucial for the localization of adherens junction components to the cell periphery...
  37. ncbi Phosphorylation of the regulatory subunit of smooth muscle protein phosphatase 1M at Thr850 induces its dissociation from myosin
    Guillermo Velasco
    Division of Signal Transduction Therapy, School of Life Sciences, University of Dundee, DD1 5EH, Dundee, UK
    FEBS Lett 527:101-4. 2002
    ..Here, we show that the phosphorylation of Thr850 by Rho kinase dissociates PP1M from myosin, providing a second mechanism by which myosin phosphatase activity is inhibited...
  38. pmc Phosphorylation of the myosin phosphatase targeting subunit and CPI-17 during Ca2+ sensitization in rabbit smooth muscle
    Toshio Kitazawa
    Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, DC 20007, USA
    J Physiol 546:879-89. 2003
    ....
  39. ncbi Structural insights into the interaction of ROCKI with the switch regions of RhoA
    Radovan Dvorsky
    Max Planck Institute fuer Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    J Biol Chem 279:7098-104. 2004
    ..The identified ROCK-binding site of RhoA strikingly supports the assumption of a common consensus-binding site for effector recognition...
  40. ncbi Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities
    Fabrice Le Boeuf
    Le Centre de Recherche en Cancérologie de l Université Laval, L Hôtel Dieu de Québec, Quebec G1R 2J6, Canada
    J Biol Chem 279:39175-85. 2004
    ..Our findings underscore for the first time the key role played by the VEGFR2-HSP90-RhoA-ROCK-FAK/Tyr(407) pathway in transducing the VEGF signal that leads to the assembly of focal adhesions and endothelial cell migration...
  41. ncbi Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion
    Simon Wilkinson
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 7:255-61. 2005
    ..Our data show that a Cdc42-MRCK signal mediates myosin-dependent cell motility and highlight convergence between Rho and Cdc42 signalling...
  42. ncbi Regulation of PTEN by Rho small GTPases
    Zhong Li
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Nat Cell Biol 7:399-404. 2005
    ..Furthermore, we have identified key residues on PTEN that are required for its regulation by the small GTPase, and show that small GTPase-mediated regulation of PTEN has a significant role in the regulation of chemotaxis...
  43. ncbi The structure of dimeric ROCK I reveals the mechanism for ligand selectivity
    Marc Jacobs
    Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139, USA
    J Biol Chem 281:260-8. 2006
    ..Hydroxyfasudil, a metabolite of fasudil, may be selective for ROCK over PKA through a reversed binding orientation...
  44. ncbi Inhibition of motile and invasive properties of ovarian cancer cells by ASODN against Rho-associated protein kinase
    Zhiqiang Han
    Cancer Biology Research Center, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, P R China
    Cell Commun Adhes 12:59-69. 2005
    b>p160ROCK, a kinase effector of Rho GTPase mediating RhoA-induced assembly of focal adhesions and stress fibers, plays an important role in the invasive process of various tumor cells...
  45. ncbi Plexin-B1 utilizes RhoA and Rho kinase to promote the integrin-dependent activation of Akt and ERK and endothelial cell motility
    John R Basile
    Oral and Pharyngeal Cancer Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:34888-95. 2007
    ..These findings provide evidence that Plexin-B1 promotes endothelial cell motility through RhoA and ROK by regulating the integrin-dependent signaling networks that result in the activation of PI3K and Akt...
  46. doi PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE
    Sophie Pinner
    Tumour Cell Biology Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Nat Cell Biol 10:127-37. 2008
    ..Depletion of PDK1 alters the localization of ROCK1 and reduces its ability to drive cortical acto-myosin contraction...
  47. doi Placenta growth factor, PLGF, influences the motility of lung cancer cells, the role of Rho associated kinase, Rock1
    Jinfeng Chen
    Department of Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, China
    J Cell Biochem 105:313-20. 2008
    ..Immunocytochemistry and Western blotting further revealed that the expression of ROCK1, Rho associated kinase, was also reduced in A549(DeltaPlGF) cells, in comparison with wild-type and control cells...
  48. pmc Deleted in liver cancer 1 (DLC1) negatively regulates Rho/ROCK/MLC pathway in hepatocellular carcinoma
    Carmen Chak Lui Wong
    Department of Pathology, SH Ho Foundation Research Laboratory and Jockey Club Clinical Research Centre, The University of Hong Kong, Hong Kong, China
    PLoS ONE 3:e2779. 2008
    ..In the present study, we aimed to investigate the effects of DLC1 on Rho/ROCK signaling pathway in hepatocellular carcinoma (HCC)...
  49. doi The Diaphanous-related Formin FHOD1 associates with ROCK1 and promotes Src-dependent plasma membrane blebbing
    Sebastian Hannemann
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:27891-903. 2008
    ..we report that FHOD1 physically associates via the N-terminal part of its FH2 domain with the central domain of ROCK1. Although FHOD1 does not affect the kinase activity of ROCK1, the DRF is an efficient substrate for ..
  50. ncbi The expression and prognostic value of ROCK I and ROCK II and their role in human breast cancer
    Jane Lane
    Metastasis and Angiogenesis Research Group, University Department of Surgery, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Int J Oncol 33:585-93. 2008
    ..This, together with significant correlation between ROCKI and poor outcome in clinical breast cancer, indicates that it is a potential target in human breast cancer...
  51. ncbi Rho-ROCK-dependent ezrin-radixin-moesin phosphorylation regulates Fas-mediated apoptosis in Jurkat cells
    Marylise Hebert
    Institut National de la Santé et de la Recherche Médicale U749, Faculte de Pharmacie, Chatenay Malabry, France
    J Immunol 181:5963-73. 2008
    ..Thus, phosphorylation of E/M by ROCK is involved in the early steps of apoptotic signaling following Fas triggering and regulates apoptosis induction...
  52. pmc Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structure
    David Komander
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 27:3175-85. 2008
    ..We propose that ROCK-I phosphorylation of RhoE forms part of a feedback loop to regulate RhoA signalling...
  53. pmc Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage
    Pat P Ongusaha
    Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
    Sci Signal 1:ra14. 2008
    ..of the JNK-interacting protein 3 (JIP-3) scaffolding protein as an interacting partner of Rho-associated kinase 1 (ROCK1), as determined by tandem affinity protein purification...
  54. pmc ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells
    Yuepeng Wang
    Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    Circ Res 104:531-40. 2009
    ..Two ROCK isoforms, ROCK1 and ROCK2, are expressed in many tissues, yet the isoform-specific roles of ROCK1 and ROCK2 in vascular smooth ..
  55. pmc Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation
    Frances E Lock
    School of Biosciences, University of Birmingham, Birmingham, United Kingdom
    PLoS ONE 4:e8190. 2009
    ..The mammalian serine/threonine kinases (ROCK1 and ROCK2) are well-characterised downstream effectors of the small GTPase RhoA...
  56. ncbi Rho-kinase inhibitor, fasudil, suppresses glioblastoma cell line progression in vitro and in vivo
    Lin Deng
    Department of Neurosurgery, Qilu Hospital of Shandong University, Shandong Province, China
    Cancer Biol Ther 9:875-84. 2010
    ..The Rho/ROCK signaling pathway may prove to be a promising target in anti-tumor therapy. Fasudil may be an attractive anti-tumor drug candidate for the treatment of GBM...
  57. pmc Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
    Mutsuki Amano
    Department of Cell Pharmacology, Nagoya University, Showa Ku, Japan
    Cytoskeleton (Hoboken) 67:545-54. 2010
    ..This review focuses on the structure, function, and modes of activation and action of Rho-kinase...
  58. pmc Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases invasion ability in human clear cell renal cell carcinoma
    K Ueno
    Department of Urology, San Francisco Veterans Affairs Medical Center, University of California, San Francisco, 4150 Clement Street, San Francisco, CA 94121, USA
    Br J Cancer 104:308-15. 2011
    ..The purpose of this study was to identify new tumour suppressor microRNAs (miRs) in clear cell renal cell carcinoma (ccRCC), carry out functional analysis of their suppressive role and identify their specific target genes...
  59. doi ROCK1 as a potential therapeutic target in osteosarcoma
    Xianzhe Liu
    Department of Orthopaedic Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Orthop Res 29:1259-66. 2011
    ..cell growth and proliferation have identified the Rho-associated coiled-coil containing protein kinase 1 (ROCK1) as a possible hit...
  60. pmc Crystal structure of a coiled-coil domain from human ROCK I
    Daqi Tu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 6:e18080. 2011
    ..Here we present the crystal structure of a large section of the central coiled-coil domain of human ROCK I (amino acids 535-700)...
  61. doi ROCK and JAK1 signaling cooperate to control actomyosin contractility in tumor cells and stroma
    Victoria Sanz-Moreno
    Oncogene Team, Institute of Cancer Research, Section of Cell and Molecular Biology, Cancer Research UK Tumour Cell Signalling Unit, 237 Fulham Road, London SW3 6JB, UK
    Cancer Cell 20:229-45. 2011
    ..Strikingly, actomyosin contractility itself positively modulates activity of the transcription factor STAT3 downstream of JAK1, demonstrating positive feedback within the signaling network...
  62. doi MicroRNA-148a suppresses tumor cell invasion and metastasis by downregulating ROCK1 in gastric cancer
    Biqiang Zheng
    Department of Gastric Cancer and Soft Tissue Sarcomas, Fudan University Shanghai Cancer Center and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    Clin Cancer Res 17:7574-83. 2011
    ..MicroRNAs (miRNA) have been documented playing a critical role in cancer development and progression. In this study, we investigate the role of miR-148a in gastric cancer metastasis...
  63. pmc MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis
    Yasuyuki Hosono
    Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan
    EMBO J 31:481-93. 2012
    ..of LIM domain kinase (LIMK), unexpectedly through its direct physical interaction with Rho kinase 1 (ROCK1) rather than with RLC...
  64. pmc Interplay between β1-integrin and Rho signaling regulates differential scattering and motility of pancreatic cancer cells by snail and Slug proteins
    Mario A Shields
    Division of Hematology Oncology, Department of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 287:6218-29. 2012
    ..In contrast, inhibiting Rho-associated kinase ROCK1/2 increased migration and scattering of Slug-expressing cells in three-dimensional collagen and thus phenocopied ..
  65. doi HIV-1 matrix protein p17 binds to the IL-8 receptor CXCR1 and shows IL-8-like chemokine activity on monocytes through Rho/ROCK activation
    Cinzia Giagulli
    Section of Microbiology, Department of Experimental and Applied Medicine, School of Medicine, University of Brescia, Italy
    Blood 119:2274-83. 2012
    ..Recent studies have highlighted the role of IL-8 and CXCR1 in HIV-1 replication and AIDS pathogenesis. Our findings herein call for an exploration of the therapeutic potential of blocking the p17/IL-8/CXCR1 axis in HIV-1 infection...
  66. pmc Differential regulation of adhesion complex turnover by ROCK1 and ROCK2
    Frances E Lock
    School of Biosciences, University of Birmingham, Edgbaston, United Kingdom
    PLoS ONE 7:e31423. 2012
    b>ROCK1 and ROCK2 are serine/threonine kinases that function downstream of the small GTP-binding protein RhoA...
  67. ncbi Inhibition of RhoA/ROCK signaling pathway promotes the apoptosis of gastric cancer cells
    Xiao Tao Xu
    Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, China
    Hepatogastroenterology 59:2523-6. 2012
    ..Ras homologue A (RhoA) plays a crucial role in the proliferation, apoptosis,adhesion and migration of gastric cancer cells. Rho associated kinase (ROCK) is an effector protein of RhoA...
  68. pmc RKI-1447 is a potent inhibitor of the Rho-associated ROCK kinases with anti-invasive and antitumor activities in breast cancer
    Ronil A Patel
    Drug Discovery Department, H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA
    Cancer Res 72:5025-34. 2012
    The Rho-associated kinases ROCK1 and ROCK2 are critical for cancer cell migration and invasion, suggesting they may be useful therapeutic targets...
  69. pmc ROCK1 and ROCK2 are required for non-small cell lung cancer anchorage-independent growth and invasion
    Dominico Vigil
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Cancer Res 72:5338-47. 2012
    Evidence is emerging that the closely related ROCK1 and ROCK2 serine/threonine kinases support the invasive and metastatic growth of a spectrum of human cancer types. Therefore, inhibitors of ROCK are under preclinical development...
  70. pmc RhoA modulates functional and physical interaction between ROCK1 and Erk1/2 in selenite-induced apoptosis of leukaemia cells
    F Li
    State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medicine Sciences and School of Basic Medicine, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
    Cell Death Dis 4:e708. 2013
    ..RhoA, regulating Rho-associated coiled-coil-forming kinase 1 (ROCK1), modulates multiple cell functions, including malignant transformation, metastasis and cell death...
  71. pmc Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress
    Aditi U Gurkar
    Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Nat Commun 4:2189. 2013
    The Ser/Thr Rho kinase 1 (ROCK1) is known to have major roles in a wide range of cellular activities, including those involved in tumour metastasis and apoptosis...
  72. doi miR-335 suppresses migration and invasion by targeting ROCK1 in osteosarcoma cells
    Yong Wang
    Department of Orthopaedics, Sheng Jing Hospital of China Medical University, Shenyang, 110004, Liaoning, People s Republic of China
    Mol Cell Biochem 384:105-11. 2013
    ..Moreover, we also showed that ROCK1 was negatively regulated by miR-335 at the posttranscriptional level, via a specific target site within the 3'UTR ..
  73. doi ROCK has a crucial role in regulating prostate tumor growth through interaction with c-Myc
    C Zhang
    1 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing, China 2 Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China
    Oncogene 33:5582-91. 2014
    ..We provided evidence demonstrating that ROCK1 directly interacted with and phosphorylated c-Myc, resulting in stabilization of the protein and activation of its ..
  74. pmc MicroRNA 135a suppresses lymph node metastasis through down-regulation of ROCK1 in early gastric cancer
    Ji Young Shin
    Center for Gastric Cancer, National Cancer Center, Goyang, Korea Department of life Science and Biotechnology, Paichai University, Daejeon, Korea
    PLoS ONE 9:e85205. 2014
    ..b>ROCK1 was a target of miRNA-135a and its expression was inversely correlated to that of miRNA-135a...
  75. pmc The mammalian formin FHOD1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells
    Ryu Takeya
    Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    EMBO J 27:618-28. 2008
    ....
  76. doi Regulation of autophagosome formation by Rho kinase
    Andrzej Mleczak
    Beatson Institute for Cancer Research, Glasgow, G61 1BD, UK
    Cell Signal 25:1-11. 2013
    ..We previously searched for novel autophagy regulators and identified Rho-kinase 1 (ROCK1) as a candidate. Here, we show that activated ROCK1 inhibits autophagy in human embryonic kidney 293 cells...
  77. pmc The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
    ....
  78. pmc RhoE binds to ROCK I and inhibits downstream signaling
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    Mol Cell Biol 23:4219-29. 2003
    ..Here, we show that RhoE binds ROCK I but none of the other RhoA targets tested...
  79. pmc Modulation of statin-activated shedding of Alzheimer APP ectodomain by ROCK
    Steve Pedrini
    Farber Institute for Neurosciences, Thomas Jefferson University Philadelphia, Pennsylvania, USA
    PLoS Med 2:e18. 2005
    ..However, the possibility that these enzymes play a role in statin-stimulated shedding has been excluded, leading us to investigate whether the Rho/ROCK1 protein phosphorylation pathway might be involved.
  80. ncbi FilGAP, a Rho- and ROCK-regulated GAP for Rac binds filamin A to control actin remodelling
    Yasutaka Ohta
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 8:803-14. 2006
    ..FilGAP is, therefore, a mediator of the well-established antagonism of Rac by RhoA that suppresses leading edge protrusion and promotes cell retraction to achieve cellular polarity...
  81. ncbi Organized migration of epithelial cells requires control of adhesion and protrusion through Rho kinase effectors
    Ann M Hopkins
    Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30322, USA
    Am J Physiol Gastrointest Liver Physiol 292:G806-17. 2007
    ....
  82. pmc RhoE is a pro-survival p53 target gene that inhibits ROCK I-mediated apoptosis in response to genotoxic stress
    Pat P Ongusaha
    Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Curr Biol 16:2466-72. 2006
    ..We found that RhoE inhibits ROCK I (Rho-associated kinase I) activity during genotoxic stress and thereby suppresses apoptosis...
  83. pmc Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation
    Jieya Shao
    Departments of Neurology and Cellular and Molecular Pharmacology, UCSF, San Francisco, California 94143, USA
    Mol Cell Biol 28:5196-208. 2008
    ..identified profilin, a small actin-binding factor that also interacts with Htt, as being a direct target of the ROCK1 isoform...
  84. pmc Heart-specific small subunit of myosin light chain phosphatase activates rho-associated kinase and regulates phosphorylation of myosin phosphatase target subunit 1
    Daisuke Shichi
    Department of Molecular Pathogenesis, Medical Research Institute, School of Biomedical Science, Tokyo Medical and Dental University, Bunkyo ku, Tokyo 113 8510, Japan
    J Biol Chem 285:33680-90. 2010
    ..In addition, hHS-M(21) bound ROCK and enhanced the ROCK activity. These findings suggest that hHS-M(21) is a heart-specific effector of ROCK and plays a regulatory role in the MYPT1 phosphorylation at Thr-696 by ROCK...
  85. ncbi Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells
    Najma Begum
    Diabetes Research Laboratory, Winthrop University Hospital, Mineola, New York 11501, USA
    J Biol Chem 277:6214-22. 2002
    ....
  86. ncbi Expression and modulation of Rho kinase in human pregnant myometrium
    C J Moran
    Department of Obstetrics and Gynaecology, National University of Ireland Galway, Clinical Science Institute, University College Hospital, Newcastle Road, Galway, Ireland
    Mol Hum Reprod 8:196-200. 2002
    ..The aims of this study were to investigate the expression of RhoA, and the Rho kinases ROCK I and ROCK II in human pregnant myometrium, to evaluate the effects of Rho kinase inhibition on pregnant human ..
  87. ncbi p80 ROKalpha binding protein is a novel splice variant of CRMP-1 which associates with CRMP-2 and modulates RhoA-induced neuronal morphology
    Thomas Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, 30 Medical Drive, 117609, Singapore
    FEBS Lett 532:445-9. 2002
    ..p80 CRMP-1 and CRMP-2 may be modulators of RhoA-dependent signaling, through interaction with and regulation of ROKalpha...
  88. ncbi Regulation of myosin phosphatase through phosphorylation of the myosin-binding subunit in platelet activation
    K Nakai
    2nd Department of Internal Medicine, Mie University School of Medicine, Tsu Mie, Japan
    Blood 90:3936-42. 1997
    ..These findings suggest a putative mechanism for agonist-induced regulation of myosin phosphatase activity in platelets...
  89. doi Roles of Necl-5/poliovirus receptor and Rho-associated kinase (ROCK) in the regulation of transformation of integrin alpha(V)beta(3)-based focal complexes into focal adhesions
    Yuichi Nagamatsu
    Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan
    J Biol Chem 283:14532-41. 2008
    ..We found that inhibition of Necl-5 expression and expression of a constitutively active mutant of ROCK1 enhanced, whereas treatment with a ROCK inhibitor Y-27632 inhibited the transformation of focal complexes into ..
  90. ncbi Contribution of protease-activated receptors 1 and 4 and glycoprotein Ib-IX-V in the G(i)-independent activation of platelet Rap1B by thrombin
    Paolo Lova
    Center of Excellence in Applied Biology, Department of Biochemistry, University of Pavia, via Bassi 21, 27100 Pavia
    J Biol Chem 279:25299-306. 2004
    ..By contrast, inhibition of MAP kinases or p160ROCK, which have been shown to be activated upon thrombin binding to GPIb-IX-V, did not affect agonist-induced ..
  91. doi 2,3,5-Trisubstituted pyridines as selective AKT inhibitors-Part I: Substitution at 2-position of the core pyridine for ROCK1 selectivity
    Hong Lin
    Oncology Medicinal Chemistry, GlaxoSmithKline, 1250 S Collegeville, Rd, Collegeville, PA 19426, United States
    Bioorg Med Chem Lett 20:673-8. 2010
    2,3,5-Trisubstituted pyridines have been designed as potent AKT inhibitors that are selective against ROCK1 based on the comparison between AKT and ROCK1 structures...
  92. pmc TGF-beta1-induced plasminogen activator inhibitor-1 expression in vascular smooth muscle cells requires pp60(c-src)/EGFR(Y845) and Rho/ROCK signaling
    Rohan Samarakoon
    Albany Medical College, Center for Cell Biology and Cancer Research, MC 165, 47 New Scotland Avenue, Albany, NY 12208, USA
    J Mol Cell Cardiol 44:527-38. 2008
    ..expression construct or pretreatment of VSMC with C3 transferase (a Rho inhibitor) or Y-27632 (an inhibitor of p160ROCK, a downstream effector of Rho) also dramatically attenuated the TGF-beta1-initiated PAI-1 inductive response...
  93. doi Identification of potential therapeutic targets in hypertension-associated bladder dysfunction
    Makoto Yono
    Department of Urology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    BJU Int 105:877-83. 2010
    ..To investigate differential gene expression profiles in the bladder of spontaneously hypertensive rat (SHR), as the underlying mechanisms involved in hypertension-associated bladder dysfunction remain to be clarified...
  94. doi Potentiation of nerve growth factor-induced neurite outgrowth by the ROCK inhibitor Y-27632: a possible role of IP₃ receptors
    Takahiko Minase
    Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Inohana, Chiba, Japan
    Eur J Pharmacol 648:67-73. 2010
    ..The results of double-staining immunocytochemistry suggested that both ROCK1 and type-1 IP₃ receptors may be co-localized in the cell body of PC12 cells...
  95. doi Participation of Rho-associated kinase in electrical stimulated and acetylcholine-induced contraction of feline esophageal smooth muscle
    Sun Young Park
    Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul 156 756, Republic of Korea
    Eur J Pharmacol 607:220-5. 2009
    ..cyclohexanecarboxamide dihydrochloride], ROCK inhibitor, and specific antibodies to ROCK1 and ROCK2 proteins, which are two isoforms of ROCK, were used...
  96. ncbi A p160ROCK-specific inhibitor, Y-27632, attenuates rat hepatic stellate cell growth
    H Iwamoto
    Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Hepatol 32:762-70. 2000
    b>p160ROCK, a serine/threonine protein kinase, is a direct RhoA target mediating RhoA-induced assembly of focal adhesions and stress fibers...
  97. pmc ROCK inhibitor (Y27632) increases apoptosis and disrupts the actin cortical mat in embryonic avian corneal epithelium
    Kathy K H Svoboda
    Department of Biomedical Science, Texas A and M Health Science Center, Baylor College of Dentistry, Dallas, Texas 75246, USA
    Dev Dyn 229:579-90. 2004
    ..In conclusion, ECM molecules decreased apoptosis markers and inhibiting the ROCK pathway blocked ECM stimulated actin cortical mat reformation and increased apoptosis in embryonic corneal epithelial cells...
  98. pmc Human neural progenitors express functional lysophospholipid receptors that regulate cell growth and morphology
    Jillian H Hurst
    Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, USA
    BMC Neurosci 9:118. 2008
    ..The goal of this study was to determine if hES-NEP cells express functional lysophospholipid receptors, and if activation of these receptors mediates cellular responses critical for neural development...
  99. doi Rho-kinase 2 is frequently overexpressed in hepatocellular carcinoma and involved in tumor invasion
    Carmen Chak Lui Wong
    Department of Pathology, Liver Cancer and Hepatitis Research Laboratory, The University of Hong Kong, Hong Kong
    Hepatology 49:1583-94. 2009
    ..We first examined the expression of the two Rho-kinases (ROCK1 and ROCK2) in human HCC, and found that ROCK2 was frequently overexpressed in primary HCCs (22/41 [53.66%])...
  100. doi Extraction of membrane cholesterol disrupts caveolae and impairs serotonergic (5-HT2A) and histaminergic (H1) responses in bovine airway smooth muscle: role of Rho-kinase
    Bettina Sommer
    Departamento de Investigacion en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico
    Can J Physiol Pharmacol 87:180-95. 2009
    ..The presence of caveolin-1 (Cav-1), H1, 5-HT(2A), and ROCK1 was corroborated by immunoblotting of membrane fractions from sucrose gradients and by confocal microscopy...
  101. pmc Inhibition of caspase-mediated anoikis is critical for basic fibroblast growth factor-sustained culture of human pluripotent stem cells
    Xiaofang Wang
    University of Connecticut Stem Cell Institute, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Biol Chem 284:34054-64. 2009
    ..of ERK and AKT and associated with inhibition of Bcl-2-interacting mediator of cell death and the caspase-ROCK1-myosin signaling. Anoikis is independent of pre-detachment apoptosis and differentiation of the cells...

Research Grants42

  1. Rho Kinase and Airway Hyperresponsiveness
    Stephanie A Shore; Fiscal Year: 2013
    ..Two ROCK isoforms exist, ROCK1 and ROCK2, that are differentially expressed, may be differentially regulated, and differ in some of the ..
  2. MATRIX MEDIATORS OF WOUND HEALING
    Paul J Higgins; Fiscal Year: 2010
    ..USF, furthermore, formed transient complexes with pERK and interference with EGFR, ppSO0"5"1 or p160ROCK activity or ERK/p38 signaling ablated induced PAI-1 expression...
  3. Young Bum Kim; Fiscal Year: 2016
    ..We found that ROCK1 deficiency causes systemic insulin resistance in vivo by impairing insulin signaling in skeletal muscle...
  4. JAMES KUANG JAN LIAO; Fiscal Year: 2014
    ..Two distinct ROCK isoforms, ROCK1 and ROCK2, have been identified, however, their role in energy metabolism and obesity are not known...
  5. JAMES KUANG JAN LIAO; Fiscal Year: 2014
    ..The Rho/Rho-associated coiled-coil forming kinases (ROCK1 and ROCK2) are important regulators of the actin cytoskeleton...
  6. ROCK inhibitor suppression of GVHD with retention of GVL response
    Sujatha Iyengar; Fiscal Year: 2012
    ..Our approach may accomplish this by inhibition of Rho associated coiled coil kinases (ROCK1&2) enzymes crucial for cytoskeletal function during leukocyte motility and loosening of endothelial and ..
  7. Lei Wei; Fiscal Year: 2016
    ..In addition, the two members of the ROCK family, ROCK1 and ROCK2, are inhibited by ROCK inhibitors with equal potency, and little is known about ROCK isoform functions ..
  8. Investigating the RhoA/ROCK pathway for the treatment of Alzheimer's disease
    JEREMY HARTFORD HERSKOWITZ; Fiscal Year: 2013
    ..The principle downstream effectors of RhoA are the Rho-associated coiled-coil containing protein kinases (ROCK), ROCK1 and ROCK2...
  9. THOMAS PATRICK LOUGHRAN; Fiscal Year: 2016
    ..Constitutive overexpression of Rac1, PAK3, RhoB and ROCK1, potential downstream effectors of S1PR5, is observed in leukemic NK cells...
  10. Qun Lu; Fiscal Year: 2014
    ..We have identified that suppression of RhoA signaling by p160ROCK/Rho kinase inhibitor Y-27632, which inhibits cancer cell motility and invasion, can reverse anti-cancer drug-..
  11. Qing Mei Wang; Fiscal Year: 2016
    ..have nonspecific effects on other signaling pathways, and cannot distinguish the effects of the two ROCK isoforms, ROCK1 and ROCK2...
  12. JOYCE MARIE SLINGERLAND; Fiscal Year: 2014
    ..vitro and p27T157AT198A bound RhoA poorly, suggesting that T198 phosphorylation may increase cell motility via Rho-ROCK1 inhibition. p27 also appears to mediate PI3K driven metastasis...
  13. Paul W Spearman; Fiscal Year: 2016
    ..Aim 3 will examine a related area, the contribution of ROCK1-LIMK1-cofilin pathway to particle budding and release, and will define the role of related actin regulatory ..
  14. Investigating the role of ROCK1 in epithelial cell inflammatory responses
    Dennis W McGee; Fiscal Year: 2010
    ..These experiments will be done using the RNA interference (RNAi) technique to knockdown ROCK1 and/or 2 expression in IEC cell lines...
  15. Zhaoyong Hu; Fiscal Year: 2016
    ..In this proposed project, I plan to study ROCK1 as a key molecule linked both to UPS activation and mitochondrial dysfunction...
  16. Jiang Chang; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): We identified Rho kinase ROCK1 as caspase-3 target in human failing hearts. The cleavage resulted in a constitutively active Rho kinase, ROCK?1...
  17. Targeted Rho kinase-1 KO Mouse Model for Vascular Smooth Muscle Remodeling
    DENIZ TOKSOZ-EXLEY; Fiscal Year: 2013
    ..On this basis, Specific Aim 1 will generate a unique vascular smooth muscle-targeted ROCK1 gene knock-out (KO) mouse strain. Specific Aim 2 will characterize the phenotype of the targeted KO strain...
  18. John T Li; Fiscal Year: 2016
    ..permeability changes in experimental ALI;(3) recombinant IL-17A stimulates increased protein expression of ROCK1, a downsteam effector of RhoA signaling, and increases permeability of pulmonary endothelial cell monolayers;and (..
  19. CONTRACTILE PROTEIN ACCUMULATION IN AIRWAY SMOOTH MUSCLE
    Julian Solway; Fiscal Year: 2002
    ..iii) Rho family GTPases activate SRF through a downstream intermediate, Rho kinase (p160ROCK-1). SA number 2...
  20. RHOA KINASES IN CARDIAC HYPERTROPHY
    Robert Schwartz; Fiscal Year: 2003
    ..In addition, we propose that the Ser/Thr kinase P160rock, recently identified as a downstream effector of Rho1A, is the primary signaling molecule mediating hypertrophic ..
  21. Rho Kinase in Mammalian Cardiac Development
    Lei Wei; Fiscal Year: 2007
    ..We observed that both p160ROCK and ROKalpha, two members of the Rho kinase family, are expressed in early mouse embryos before the onset of ..
  22. Regulation of Polyglutamine Aggregation and Toxicity
    Marc Diamond; Fiscal Year: 2009
    ..Previous work demonstrated that Y-27632, an inhibitor of the rho-associated kinase p160ROCK, reduced polyglutamine aggregation and toxicity in cell and Drosophila models...
  23. Prefrontal cortex and memory storage
    Pramod K Dash; Fiscal Year: 2010
    ....
  24. Working memory deficits following brain trauma
    Pramod Dash; Fiscal Year: 2009
    ..An understanding of the mechanisms by which TBI alters dopamine signaling is critical for the development of mechanism-based intelligent pharmacological treatments for working memory deficits for brain trauma patients. ..
  25. BIOLOGY OF THE EMBRYONIC CORNEAL EPITHELIUM
    Kathy Svoboda; Fiscal Year: 2001
    ..In summary, these experiments in our well defined corneal epithelial model will focus on how ECM mediated signal transduction stimulates actin bundle formation and also save the cells from programmed cell death. ..
  26. LONG TERM HIPPOCAMPAL MEMORY
    Pramod Dash; Fiscal Year: 2001
    ..Understanding the molecular basis of memory storage will be of value in the future development of pharmacological agents to treat amnesia associated with neurological disorders and other mental health related problems. ..
  27. G PROTEIN MEDIATED CA++ SENSITIZATION IN SMOOTH MUSCLE
    Toshio Kitazawa; Fiscal Year: 2002
    ....
  28. NUCLEOTIDE-MEDIATED LEUKOCYTE STIMULATION
    Satya Kunapuli; Fiscal Year: 2004
    ..The studies proposed in this application will also help us understand the mechanisms of neutrophil activation and contribute to the knowledge of better treatments and to the design of therapeutics for inflammatory diseases. ..
  29. Role of Rho Kinase in Aqueous Humor Outflow Pathway
    P Rao; Fiscal Year: 2004
    ..Identification of specific signaling mechanisms regulating function of outflow pathway tissues could enable the design of target-specific approaches to the treatment of glaucoma. ..
  30. Plasmid Delivery & Expression in Embryonic Eye Tissues
    Kathy Svoboda; Fiscal Year: 2005
    ..This project has a high risk because neither "two-in-one" vectors, nor transfection into whole tissues has been accomplished. ..
  31. TGFBeta Signaling in the Bladder Stroma
    Neil Bhowmick; Fiscal Year: 2005
    ..Results from these studies are directly relevant to potential non-invasive strategies in treating one of the most common pathologies of the bladder. ..
  32. Regulation of endothelial permeability via rhoA/ROCK
    JEROME BRESLIN; Fiscal Year: 2006
    ..We will also investigate how rhoA/ROCK inhibition affects these PMN-stimulated changes. This study will greatly increase our understanding of endothelial barrier regulation. [unreadable] [unreadable]..
  33. ICP-MS Instrument for Baylor College of Dentistry
    Kathy Svoboda; Fiscal Year: 2006
    ..We have identified two laboratories in the Dallas area that have appropriate instruments, but they cannot analyze our samples (see correspondence). [unreadable] [unreadable]..
  34. Mechanism of Ca2+ Sensitization in Smooth Muscle
    Toshio Kitazawa; Fiscal Year: 2006
    ..abstract_text> ..
  35. Role of PI3-Kinase in Estrogen-induced eNOS Activation
    James Liao; Fiscal Year: 2007
    ..These results may explain some of the actions of selective estrogen receptor modulators (SERMs) and provide a therapeutic basis for using estrogens in cardiovascular disease. ..
  36. In vivo role of Rho-kinase in glucose metabolism
    Young Bum Kim; Fiscal Year: 2007
    ..These experiments could lead to the identification of a potential target for new diabetes drugs. [unreadable] [unreadable] [unreadable]..
  37. Platelet ADP receptors
    Satya Kunapuli; Fiscal Year: 2008
    ..We further propose to evaluate the relative contribution of the P2Y1 and the P2Y12 receptors to thrombus formation, using P2Y1 or P2Y12 deficient mice and treatment with antagonists and models of in vivo thrombosis and thromboembolism. ..
  38. Endothelial Akt in Vascular Injury
    James Liao; Fiscal Year: 2009
    ..Three specific aims are proposed, which will study the role of endothelial Akt in models of vascular injury and atherosclerosis. ..
  39. Functional G Protein pathways in platelet activation
    Satya P Kunapuli; Fiscal Year: 2010
    ..These studies will enhance our understanding of the signaling pathways and their role in platelet activation, and might identify potential newer targets for the treatment of thrombosis. ..
  40. Activation of eNOS by Steroid Hormones
    James Liao; Fiscal Year: 2006
    ....
  41. MECHANISMS OF MEMORY DEFICITS FOLLOWING BRAIN INJURY
    Pramod Dash; Fiscal Year: 2006
    ..Furthermore, this project will broaden our understanding of the pathogenesis of neurodegenerative diseases such as stroke and Alzheimer's disease. ..
  42. Regulation of gp91phox by Anaplasma phagocytophila
    Venetta Thomas; Fiscal Year: 2007
    ..Aim 2. To define the effect of A. phagocytophila infection on the activity of the activator proteins and their binding capacity of the gp91phox promoter. ..