RhoGDI

Summary

Gene Symbol: RhoGDI
Description: Rho GDP dissociation inhibitor alpha
Alias: GDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1, rho GDP-dissociation inhibitor 1, GDP-dissociation inhibitor, aplysia RAS-related 1, Rho GDP dissociation inhibitor (GDI) alpha, epididymis secretory sperm binding protein Li 47e
Species: human

Top Publications

  1. ncbi Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1
    L Y Lian
    Department of Biochemistry and Biological NMR Centre, University of Leicester, Leicester, LE1 7RH, UK
    Structure 8:47-55. 2000
  2. ncbi The HIV-1 vpr protein induces anoikis-resistance by modulating cell adhesion process and microfilament system assembly
    P Matarrese
    Department of Ultrastructures, Instituto Superiore di Sanita, Viale Regina Elena, 299 00161 Rome, Italy
    Cell Death Differ 7:25-36. 2000
  3. ncbi Modifications in the human T cell proteome induced by intracellular HIV-1 Tat protein expression
    Mayte Coiras
    AIDS Immunopathology Unit, National Centre of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    Proteomics 6:S63-73. 2006
  4. ncbi Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton
    H Leffers
    Institute of Medical Biochemistry, Aarhus University, Denmark
    Exp Cell Res 209:165-74. 1993
  5. ncbi Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein
    K Takahashi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
    J Biol Chem 272:23371-5. 1997
  6. ncbi Differential properties of D4/LyGDI versus RhoGDI: phosphorylation and rho GTPase selectivity
    J P Gorvel
    Centre d Immunologie INSERM CNRS de Marseille Luminy, France
    FEBS Lett 422:269-73. 1998
  7. ncbi Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI
    G R Hoffman
    Department of Molecular Medicine, Veterinary Medical Center, Cornell University, Ithaca, New York 14853, USA
    Cell 100:345-56. 2000
  8. ncbi Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI
    A P Golovanov
    Department of Biochemistry and Biological NMR Centre, University of Leicester, Leicester, LE1 7RH, UK
    J Mol Biol 305:121-35. 2001
  9. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001
  10. ncbi Crystal structure of the Rac1-RhoGDI complex involved in nadph oxidase activation
    S Grizot
    Institut de Biologie Structurale, CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Biochemistry 40:10007-13. 2001

Research Grants

  1. Celine DerMardirossian; Fiscal Year: 2016
  2. Alexander Staruschenko; Fiscal Year: 2016
  3. Role of Rho GDP dissociation inhibitors in androgen signaling in prostate cancer
    Allen C Gao; Fiscal Year: 2013
  4. Keith Burridge; Fiscal Year: 2016
  5. Friedhelm Hildebrandt; Fiscal Year: 2016
  6. MECHANISM AND FUNCTION OF PROTEIN PRENYLATION
    Mark D Distefano; Fiscal Year: 2010
  7. G Proteins and Endothelial Barrier Regulation
    Tatyana Voyno Yasenetskaya; Fiscal Year: 2004
  8. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
  9. REGULATION OF NEUTROPHIL FUNCTION
    J Lambeth; Fiscal Year: 2000
  10. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Celine DerMardirossian; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications174 found, 100 shown here

  1. ncbi Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1
    L Y Lian
    Department of Biochemistry and Biological NMR Centre, University of Leicester, Leicester, LE1 7RH, UK
    Structure 8:47-55. 2000
    ..The cytosolic guanine nucleotide dissociation inhibitors, RhoGDIs, regulate both the GDP/GTP exchange cycle and the membrane association/dissociation cycle...
  2. ncbi The HIV-1 vpr protein induces anoikis-resistance by modulating cell adhesion process and microfilament system assembly
    P Matarrese
    Department of Ultrastructures, Instituto Superiore di Sanita, Viale Regina Elena, 299 00161 Rome, Italy
    Cell Death Differ 7:25-36. 2000
    ..e. by inducing anoikis-resistance. These particular effects of vpr might enhance the homing, spreading and survival of the infected lymphocytes, thus contributing to virus persistence in the course of acute HIV-1 infection...
  3. ncbi Modifications in the human T cell proteome induced by intracellular HIV-1 Tat protein expression
    Mayte Coiras
    AIDS Immunopathology Unit, National Centre of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    Proteomics 6:S63-73. 2006
    ..In conclusion, in addition to its role in viral mRNA elongation, the proteomic approach has provided insight into the way that Tat modifies host cell gene expression...
  4. ncbi Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton
    H Leffers
    Institute of Medical Biochemistry, Aarhus University, Denmark
    Exp Cell Res 209:165-74. 1993
    ..Taken together, the results imply that Rho GDI's play a role in modulating the activity of the Rho proteins as their overexpression mimics phenotypic changes associated with the inactivation of these proteins...
  5. ncbi Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein
    K Takahashi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
    J Biol Chem 272:23371-5. 1997
    ..These results suggest that ERM as well as Rho GDI and the Rho GDP/GTP exchange factor are involved in the activation of the Rho subfamily members, which then regulate reorganization of actin filaments through the ERM system...
  6. ncbi Differential properties of D4/LyGDI versus RhoGDI: phosphorylation and rho GTPase selectivity
    J P Gorvel
    Centre d Immunologie INSERM CNRS de Marseille Luminy, France
    FEBS Lett 422:269-73. 1998
    ..b>RhoGDI is a ubiquitously expressed GDI, whereas D4/LyGDI is hematopoietic cell-specific and 10-fold less potent than ..
  7. ncbi Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI
    G R Hoffman
    Department of Molecular Medicine, Veterinary Medical Center, Cornell University, Ithaca, New York 14853, USA
    Cell 100:345-56. 2000
    The RhoGDI proteins serve as key multifunctional regulators of Rho family GTP-binding proteins. The 2.6 A X-ray crystallographic structure of the Cdc42/RhoGDI complex reveals two important sites of interaction between GDI and Cdc42...
  8. ncbi Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI
    A P Golovanov
    Department of Biochemistry and Biological NMR Centre, University of Leicester, Leicester, LE1 7RH, UK
    J Mol Biol 305:121-35. 2001
    The guanine dissociation inhibitors RhoGDI and D4GDI inhibit guanosine 5'-diphosphate dissociation from Rho GTPases, keeping these small GTPases in an inactive state...
  9. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001
    ..Targeting was regulated by binding to RhoGDI alpha in the case of RhoA, Rac1, Rac2, and Cdc42hs but not RhoB or TC10...
  10. ncbi Crystal structure of the Rac1-RhoGDI complex involved in nadph oxidase activation
    S Grizot
    Institut de Biologie Structurale, CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Biochemistry 40:10007-13. 2001
    ..Small angle neutron scattering experiments confirmed a 1:1 stoichiometry. The crystal structure of the Rac1-RhoGDI complex was determined at 2.7 A resolution...
  11. ncbi The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI
    Toshihide Yamashita
    Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nat Neurosci 6:461-7. 2003
    ..This peptide has potential as a therapeutic agent against the inhibitory cues that block regeneration in the central nervous system...
  12. ncbi Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase
    Celine DerMardirossian
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 15:117-27. 2004
    Selective activation of Rac GTPase signaling pathways requires the specific release of Rac from RhoGDI complexes. We identified a RhoGDI kinase from bovine brain as p21-activated kinase (Pak)...
  13. ncbi Uncoupling of inhibitory and shuttling functions of rho GDP dissociation inhibitors
    Estelle Dransart
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS UPR 9063, F 91198 Gif sur Yvette, France
    J Biol Chem 280:4674-83. 2005
    ..process involving the extraction of Rho from donor membranes, the formation of the inhibitory cytosolic Rho/rhoGDI complexes, and delivery of Rho to target membranes...
  14. ncbi Rho GDP dissociation inhibitor protects cancer cells against drug-induced apoptosis
    Baolin Zhang
    Laboratory of Biochemistry, Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Cancer Res 65:6054-62. 2005
    Rho GDP dissociation inhibitor (RhoGDI) plays an essential role in control of a variety of cellular functions through interactions with Rho family GTPases, including Rac1, Cdc42, and RhoA...
  15. ncbi Activity of the Bcr GTPase-activating domain is regulated through direct protein/protein interaction with the Rho guanine nucleotide dissociation inhibitor
    Soo Mi Kweon
    Section of Molecular Carcinogenesis, Division of Hematology Oncology, The Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, California 90027, USA
    J Biol Chem 283:3023-30. 2008
    ..Here, we report that in the presence of RhoGDIalpha, Bcr is unable to convert Rac-GTP to Rac-GDP because RhoGDI forms a direct protein complex with Bcr...
  16. doi RhoA-GDP regulates RhoB protein stability. Potential involvement of RhoGDIalpha
    T T Giang Ho
    Laboratory of Connective Tissues Biology, GIGA R, University of Liege, Tour de Pathologie, Sart Tilman, Belgium
    J Biol Chem 283:21588-98. 2008
    ..Our results highlight RhoGDIalpha-dependent cross-talks that regulate the stability of RhoGTPases...
  17. doi Overexpression of Rho GDP-dissociation inhibitor alpha is associated with tumor progression and poor prognosis of colorectal cancer
    Liang Zhao
    Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China
    J Proteome Res 7:3994-4003. 2008
    ..One member of GDIs was identified as RhoGDI (Rho GDP-dissociation inhibitor alpha, or RhoGDIalpha), but little is known about this protein in tumors...
  18. pmc Four-gene expression ratio test for survival in patients undergoing surgery for mesothelioma
    Gavin J Gordon
    Department of Surgery, Division of Thoracic Surgery, Harvard Medical School and Brigham and Women s Hospital, 75 Francis St, Boston, MA 02115, USA
    J Natl Cancer Inst 101:678-86. 2009
    ..In this study, we investigated the predictive value and technical assay performance of this test in patients with malignant pleural mesothelioma...
  19. pmc Differential phosphorylation of RhoGDI mediates the distinct cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion
    Zhanxiang Wang
    Herman B Wells Center for Pediatric Research, Basic Diabetes Research Group, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Biol Chem 285:6186-97. 2010
    ..Using a tandem affinity purification-based mass spectrometry screen for Cdc42 cycling factors in beta cells, RhoGDI was identified...
  20. pmc Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1
    Etienne Boulter
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 12:477-83. 2010
    ....
  21. pmc Serine 34 phosphorylation of rho guanine dissociation inhibitor (RhoGDIalpha) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion
    Athanassios Dovas
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
    J Biol Chem 285:23296-308. 2010
    ..Together with fluorescence resonance energy transfer microscopy sensing GTP-RhoA levels, the data reveal a common pathway in cell adhesion linking two essential mediators, conventional PKC and RhoA...
  22. pmc Semaphorin 5A and plexin-B3 inhibit human glioma cell motility through RhoGDIalpha-mediated inactivation of Rac1 GTPase
    Xinhua Li
    Department of Physiology, Yong Loo Lin School of Medicine, Neurobiology Ageing Program, Life Sciences Institute, National University of Singapore, Centre for Life Sciences, 28 Medical Drive, Singapore 117456
    J Biol Chem 285:32436-45. 2010
    ..for the underlying mechanism revealed direct interaction of plexin-B3 with RhoGDP dissociation inhibitor α (RhoGDIα), a negative regulator of RhoGTPases that blocks guanine nucleotide exchange and sequesters them away from the ..
  23. pmc The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac
    Fei Fei
    Section of Molecular Carcinogenesis, Division of Hematology Oncology, The Saban Research Institute of Childrens Hospital Los Angeles, CA 90027, USA
    BMC Biochem 11:48. 2010
    b>RhoGDI proteins are important regulators of the small GTPase Rac, because they shuttle Rac from the cytoplasm to membranes and also protect Rac from activation, deactivation and degradation...
  24. pmc X-linked inhibitor of apoptosis protein (XIAP) mediates cancer cell motility via Rho GDP dissociation inhibitor (RhoGDI)-dependent regulation of the cytoskeleton
    Jinyi Liu
    Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, New York 10987, USA
    J Biol Chem 286:15630-40. 2011
    ..that the regulation of cell motility by XIAP depends on its interaction with the Rho GDP dissociation inhibitor (RhoGDI) via the XIAP RING domain...
  25. pmc The 'invisible hand': regulation of RHO GTPases by RHOGDIs
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
    Nat Rev Mol Cell Biol 12:493-504. 2011
    ..The same metaphor can be used to describe the RHO-specific guanine nucleotide dissociation inhibitor (RHOGDI) family, which operates in the background, as an invisible hand, using similar forces to regulate the RHO GTPase ..
  26. pmc Phosphorylation of RhoGDI by Src regulates Rho GTPase binding and cytosol-membrane cycling
    Celine DerMardirossian
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 17:4760-8. 2006
    ..Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from RhoGDI complexes, followed by their reuptake after membrane cycling...
  27. ncbi Rho GDP dissociation inhibitor alpha interacts with estrogen receptor alpha and influences estrogen responsiveness
    Saad El Marzouk
    Department of Cell and Developmental Biology, University of Illinois, Urbana, Illinois 61801, USA
    J Mol Endocrinol 39:249-59. 2007
    ..One of these proteins, Rho guanosine diphosphate (GDP) dissociation inhibitor alpha (RhoGDI alpha), is a negative regulator of the Rho family of GTP-binding proteins...
  28. ncbi RhoGDI-3 is a new GDP dissociation inhibitor (GDI). Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG
    G Zalcman
    Unité INSERM 248, Section de Recherche, Institut Curie, 26 Rue d Ulm, 75231 Paris Cedex 05, France
    J Biol Chem 271:30366-74. 1996
    ..proteins interacting with RhoB, we identified a new mouse Rho GDP dissociation inhibitor, referenced as RhoGDI-3...
  29. ncbi Differential expression and regulation of GTPases (RhoA and Rac2) and GDIs (LyGDI and RhoGDI) in neutrophils from patients with severe congenital neutropenia
    B Kasper
    Department of Pediatric Hematology Oncology, Medical School Hannover, Hannover, Germany
    Blood 95:2947-53. 2000
    ..This study determined that the guanosine diphosphate (GDP)-dissociation inhibitor RhoGDI is overexpressed at the protein level in patients' neutrophils and that overexpression is a result of G-CSF ..
  30. doi Proteomic analysis of Legionella-containing phagosomes isolated from Dictyostelium
    Olga Shevchuk
    Institut fur Mikrobiologie, Technische Universitat Braunschweig, Braunschweig, Germany
    Int J Med Microbiol 299:489-508. 2009
    ..hackeliae revealed distinctive protein expression patterns, e.g., an abundance of RhoGDI in L. hackeliae degrading phagosomes versus little RhoGDI in L. pneumophila Corby replicative phagosomes...
  31. doi A cross-link between protein kinase A and Rho-family GTPases signaling mediates cell-cell adhesion and actin cytoskeleton organization in epithelial cancer cells
    Fernanda Leve
    Divisao de Biologia Celular, Coordenação de Pesquisa, Instituto Nacional de Cancer, Rio de Janeiro, Brazil
    J Pharmacol Exp Ther 327:777-88. 2008
    ..caused activation of Rho, as evidenced by their translocation to the membrane, an event concurrent to Rac and RhoGDI translocation, and this effect was also reverted by H-89...
  32. ncbi The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI
    K Scheffzek
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Otto Hahn Str 11, 44227 Dortmund, Germany
    Nat Struct Biol 7:122-6. 2000
    ..We have solved the crystal structure of a complex between the RhoGDI homolog LyGDI and GDP-bound Rac2, which are abundant in leukocytes, representing the cytosolic, resting pool of ..
  33. ncbi Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth
    Tamara J Richman
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, 95 Carrigan Dr, 202 Stafford Hall, Burlington, VT 05405, USA
    Curr Genet 45:339-49. 2004
    ..studies were performed in Saccharomyces cerevisiae using green fluorescent protein (GFP)-tagged Cdc42p and the RhoGDI Rdi1p...
  34. ncbi Localization of the Rho GTPases and some Rho effector proteins in the sperm of several mammalian species
    Carl C Ducummon
    Department of Animal Science, One Shields Avenue, University of California, Davis, Davis, CA 95616, USA
    Zygote 14:249-57. 2006
    ..In this study, the localization of the Rho GTPases (RhoA, RhoB, Rac1 and Cdc42) as well as the effectors RhoGDI, PI(4)P5K and ROCK-1, was determined in boar, human, rat, ram, bull and elephant sperm...
  35. ncbi Guanine nucleotide exchange regulates membrane translocation of Rac/Rho GTP-binding proteins
    G M Bokoch
    Department of Immunology, Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 269:31674-9. 1994
    GTP-binding proteins of the Rho family are maintained as cytosolic complexes with RhoGDI in resting cells, but are released and translocate to the membrane during the course of cell activation...
  36. pmc The role of ezrin-associated protein network in human sperm capacitation
    Lei Wang
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China
    Asian J Androl 12:667-76. 2010
    ..Furthermore, activated ezrin and the Rho dissociation inhibitor, RhoGDI, promote the formation of cortical cytoskeleton-polymerized actin through Rho activation...
  37. pmc cDNA cloning of a human mRNA preferentially expressed in hematopoietic cells and with homology to a GDP-dissociation inhibitor for the rho GTP-binding proteins
    J M Lelias
    Department of Medicine, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215
    Proc Natl Acad Sci U S A 90:1479-83. 1993
    ..D4 encodes a 201-amino acid protein with homology to rhoGDI, an inhibitor of GDP dissociation for the ras-homologous protein rho...
  38. pmc Facilitation of Ca(2+)-dependent exocytosis by Rac1-GTPase in bovine chromaffin cells
    Quanwen Li
    Department of Physiology, University of Michigan, Ann Arbor 48109, USA
    J Physiol 550:431-45. 2003
    ..A dominant negative Rac1 mutant (Rac1-N17) produced no effect on secretion. Expression of RhoGDI, a negative regulator of Rac1, inhibited secretory responses while overexpression of effectors of Rac1, notably, ..
  39. doi Apoptotic effects of a high performance liquid chromatography (HPLC) fraction of Antrodia camphorata mycelia are mediated by down-regulation of the expressions of four tumor-related genes in human non-small cell lung carcinoma A549 cell
    Yu Yi Chan
    Department of Biotechnology, Southern Taiwan University, Yungkang City, Tainan County, Taiwan
    J Ethnopharmacol 127:652-61. 2010
    ....
  40. pmc Recombinant Yersinia YopT leads to uncoupling of RhoA-effector interaction
    I Sorg
    Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Albert Ludwigs Universität Freiburg, D 79104 Freiburg, Germany
    Infect Immun 69:7535-43. 2001
    ..of lysate or cytosol with YopT caused inhibition of the RhoA-rhotekin interaction but led to increased RhoA-RhoGDI interaction...
  41. doi Age-related reductions in expression of serum response factor and myocardin-related transcription factor A in mouse skeletal muscles
    Kunihiro Sakuma
    Health Science Center, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Biochim Biophys Acta 1782:453-61. 2008
    ..RT)-PCR, Western blotting, and immunofluorescence microscopy, we investigated whether the amounts of RhoA, RhoGDI, SRF, MRTF-A, and MyoD in the triceps brachii and quadriceps muscles change with aging in mice...
  42. ncbi ExoS Rho GTPase-activating protein activity stimulates reorganization of the actin cytoskeleton through Rho GTPase guanine nucleotide disassociation inhibitor
    Jianjun Sun
    Medical College of Wisconsin, Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 279:42936-44. 2004
    ..The present study investigated the roles of Rho GTPase guanine nucleotide disassociation inhibitor (RhoGDI) in the reorganization of actin cytoskeleton mediated by ExoS RhoGAP...
  43. pmc Dissociation of Rac1(GDP).RhoGDI complexes by the cooperative action of anionic liposomes containing phosphatidylinositol 3,4,5-trisphosphate, Rac guanine nucleotide exchange factor, and GTP
    Yelena Ugolev
    Julius Friedrich Cohnheim Minerva Center for Phagocyte Research and the Ela Kodesz Institute of Host Defense against Infectious Diseases, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 283:22257-71. 2008
    ..In resting cells, Rac is found in the cytosol in complex with Rho GDP dissociation inhibitor (RhoGDI). NADPH oxidase assembly involves dissociation of the Rac.RhoGDI complex and translocation of Rac to the membrane...
  44. pmc Protein expression in human trabecular meshwork: downregulation of RhoGDI by dexamethasone in vitro
    Minbin Yu
    Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat Sen University, Guangzhou, P R China
    Mol Vis 16:213-23. 2010
    ..This study uses proteomic techniques to investigate the set of proteins expressed in normal human TM and to identify those differentially expressed in response to dexamethasone (DEX) treatment of TM cells (TMCs) in vitro...
  45. ncbi Phosphorylation of serine 188 protects RhoA from ubiquitin/proteasome-mediated degradation in vascular smooth muscle cells
    Malvyne Rolli-Derkinderen
    INSERM U 533, Faculte des Sciences, 2 rue de la Houssiniere, BP 92208, 44322 Nantes Cedex 3, France
    Circ Res 96:1152-60. 2005
    ..form of RhoA to proteasome-mediated degradation is because of its cytoplasmic sequestration through enhanced RhoGDI interaction...
  46. pmc Diacylglycerol kinase zeta regulates actin cytoskeleton reorganization through dissociation of Rac1 from RhoGDI
    Hanan Abramovici
    Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
    Mol Biol Cell 20:2049-59. 2009
    Activation of Rac1 GTPase signaling is stimulated by phosphorylation and release of RhoGDI by the effector p21-activated kinase 1 (PAK1), but it is unclear what initiates this potential feed-forward mechanism for regulation of Rac ..
  47. pmc Characterization of a Rac1- and RhoGDI-associated lipid kinase signaling complex
    K F Tolias
    Beth Israel Deaconess Medical Center, and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    Mol Cell Biol 18:762-70. 1998
    ..b>RhoGDI also associates with this lipid kinase complex both in vivo and in vitro, primarily via its interaction with Rac...
  48. pmc Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI
    Francisco Rivero
    Institut fur Biochemie I, Medizinische Fakultat, University of Cologne, Joseph Stelzmann Strasse 52, D 50931 Köln, Germany
    EMBO J 21:4539-49. 2002
    ..We identified two RhoGDI homologues in DICTYOSTELIUM: GDI1 shares 51-58% similarity to RhoGDIs from diverse species...
  49. ncbi The three-dimensional model of Dictyostelium discoideum racE based on the human rhoA-GDP crystal structure
    Madhavi Agarwal
    Department of Biology, Clark University, Worcester, MA 01610, USA
    J Mol Graph Model 21:3-18. 2002
    ..structural elements between models generated using various templates, such as h-rac1, h-rhoA and h-cdc42 bound to RhoGDI, all sharing only 50-55% sequence identity with racE, which suggests that this model is in all probability an ..
  50. ncbi Tumor cell pseudopodial protrusions. Localized signaling domains coordinating cytoskeleton remodeling, cell adhesion, glycolysis, RNA translocation, and protein translation
    Zongjian Jia
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3
    J Biol Chem 280:30564-73. 2005
    ..ubiquitin-mediated protein degradation, as well as protein chaperones (HSP90 and HSC70) and signaling proteins (RhoGDI and ROCK)...
  51. ncbi Radixin stimulates Rac1 and Ca2+/calmodulin-dependent kinase, CaMKII: cross-talk with Galpha13 signaling
    Guoquan Liu
    Department of Pharmacology, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 280:39042-9. 2005
    ..The N-terminal domain potentiated SRE activation induced by Galpha(13)Q226L; RhoGDI inhibited this effect. Surprisingly, the C-terminal domain also stimulated the SRE-dependent gene transcription...
  52. pmc TRAIL and Taurolidine induce apoptosis and decrease proliferation in human fibrosarcoma
    Adrien Daigeler
    Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil, Burkle de la Camp Platz 1, 44789 Bochum, Germany
    J Exp Clin Cancer Res 27:82. 2008
    ..Disseminated soft tissue sarcoma still represents a therapeutic dilemma because effective cytostatics are missing. Therefore we tested TRAIL and Tarolidine (TRD), two substances with apoptogenic properties on human fibrosarcoma (HT1080)...
  53. ncbi Loss of expression of LyGDI (ARHGDIB), a rho GDP-dissociation inhibitor, in Hodgkin lymphoma
    Liya Ma
    Department of Pathology, Schleswig Holstein University Hospitals, Kiel, Germany
    Br J Haematol 139:217-23. 2007
    The guanosine triphosphatase (GTPase) inhibitor LyGDI (ARHGDIB, Ly/D4-GDI, RhoGDIb or RhoGDI 2) is abundantly expressed in haematopoetic cells and possibly plays a role in the onset of apoptosis...
  54. pmc Gankyrin plays an essential role in Ras-induced tumorigenesis through regulation of the RhoA/ROCK pathway in mammalian cells
    Jiang Hong Man
    State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China
    J Clin Invest 120:2829-41. 2010
    ..following Ras activation increased the interaction between the RhoA GTPase and its GDP dissociation inhibitor RhoGDI, which resulted in inhibition of the RhoA effector kinase Rho-associated coiled coil-containing protein kinase (..
  55. ncbi Increased RhoGDI2 and peroxiredoxin 5 levels in asthmatic murine model of beta2-adrenoceptor desensitization: a proteomics approach
    Hua Liu
    Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China
    Chin Med J (Engl) 121:355-62. 2008
    ..It is not clear whether there are other mechanisms. This study aimed to investigate potential mechanisms of beta(2)AR desensitization...
  56. ncbi Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis
    F Bischoff
    Max Delbruck Laboratorium in der Max Planck Gesellschaft, Köln Vogelsang, Germany
    Plant Mol Biol 42:515-30. 2000
    ..As RhoGDIs are known to bind to the C-terminus of GTPases of the Rho family, we searched for Arabidopsis RhoGDI genes. We identified the AtRhoGDI1 gene and mapped it to chromosome 3. AtRhoGDI1 encodes a 22...
  57. ncbi Neurotrophin binding to the p75 receptor modulates Rho activity and axonal outgrowth
    T Yamashita
    Department of Neurobiochemistry, Max Planck Institute of Neurobiology, Martinsried, Federal Republic of Germany
    Neuron 24:585-93. 1999
    ..In vivo, axonal outgrowth was retarded in mice carrying a mutation in the p75NTR gene. These results indicate that p75NTR modulates in a ligand-dependent fashion the activity of intracellular proteins known to regulate actin assembly...
  58. ncbi p75 neurotrophin receptor signaling regulates growth cone filopodial dynamics through modulating RhoA activity
    Scott Gehler
    Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Neurosci 24:4363-72. 2004
    ..These observations suggest that neurotrophins regulate filopodial dynamics by depressing the activation of RhoA that occurs through p75NTR signaling...
  59. ncbi Gene expression profiles with cDNA microarray reveal RhoGDI as a predictive marker for paclitaxel resistance in ovarian cancers
    Tomoko Goto
    Department of Obstetrics and Gynecology, Saitama Medical College, Iruma gun, Saitama 350 0495, Japan
    Oncol Rep 15:1265-71. 2006
    ..of the ATP-binding cassette subfamily (MDR-1), Rho guanine dinucleotide phosphate dissociation inhibitor beta (RhoGDI) and insulin-like growth factor binding protein 3 (IGFBP-3) was observed in both paclitaxel-resistant cell lines...
  60. pmc In vivo dynamics of Rac-membrane interactions
    Konstadinos Moissoglu
    Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Mol Biol Cell 17:2770-9. 2006
    ..Both overexpression and small hairpin RNA-mediated suppression of RhoGDI strongly affected the amount of membrane-bound Rac but surprisingly had only slight effects on k(off)...
  61. ncbi Proteomics study reveals cross-talk between Rho guanidine nucleotide dissociation inhibitor 1 post-translational modifications in epidermal growth factor stimulated fibroblasts
    Ida Chiara Guerrera
    Department of Medicine, University College London, 5 University Street, London
    J Proteome Res 6:2623-30. 2007
    ..and serine and tyrosine phosphorylation was observed for Rho guanidine nucleotide dissociation inhibitor 1 (RhoGDI-1)...
  62. pmc Effects of lovastatin on Rho isoform expression, activity, and association with guanine nucleotide dissociation inhibitors
    Stephanie J Turner
    Department of Medicine and Cancer Center, University of California, San Diego, La Jolla, CA 92093 0652, USA
    Biochem Pharmacol 75:405-13. 2008
    ..8%. Lovastatin reduced Rho association with Rho guanine dissociation inhibitor (RhoGDI)-alpha and -beta, and prenylation-deficient Rho mutants did not associate with RhoGDI...
  63. doi The adaptive responses in several mediators linked with hypertrophy and atrophy of skeletal muscle after lower limb unloading in humans
    K Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, Toyohashi, Japan
    Acta Physiol (Oxf) 197:151-9. 2009
    ..we examined whether unilateral lower limb suspension changes the mRNA and protein levels of SRF-linked (RhoA, RhoGDI, STARS and SRF), myostatin-linked (myostatin, Smad2, Smad3 and FLRG) and Foxo-linked (P-Akt, Foxo1, Foxo3a and ..
  64. pmc RhoGDIα suppresses growth and survival of prostate cancer cells
    Yezi Zhu
    Department of Urology, University of California at Davis, Sacramento, California 95817, USA
    Prostate 72:392-8. 2012
    ..We have previously generated LNCaP-IL6+ cells by treating LNCaP cells chronically with interleukin-6 (IL-6), which have acquired the ability to grow in androgen-deprived conditions...
  65. doi RhoGDI2 confers gastric cancer cells resistance against cisplatin-induced apoptosis by upregulation of Bcl-2 expression
    Hee Jun Cho
    Department of Microbiology Research Institute of Life Science, College of Natural Sciences, Gyeongsang National University, Jinju, Republic of Korea
    Cancer Lett 311:48-56. 2011
    Rho GDP dissociation inhibitor (RhoGDI)2 has been identified as a regulator of Rho family GTPase. Recently, we suggested that RhoGDI2 could promote tumor growth and malignant progression in gastric cancer...
  66. pmc Quantitative analysis of prenylated RhoA interaction with its chaperone, RhoGDI
    Zakir Tnimov
    Department of Molecular Cell Biology, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St Lucia, Queensland 4072, Australia
    J Biol Chem 287:26549-62. 2012
    ..Membrane delivery and recycling of the Rho GTPases is mediated by Rho GDP dissociation inhibitor (RhoGDI), which forms a stable complex with prenylated Rho GTPases...
  67. doi miR-483-5p promotes invasion and metastasis of lung adenocarcinoma by targeting RhoGDI1 and ALCAM
    Qiancheng Song
    Authors Affiliations Department of Cell Biology, School of Basic Medical Sciences and Institute of Genetic Engineering, Southern Medical University, Guangzhou, China
    Cancer Res 74:3031-42. 2014
    ..Our findings reveal that miR-483-5p is a critical β-catenin-activated prometastatic miRNA and a negative regulator of the metastasis suppressors RhoGDI1 and ALCAM...
  68. doi RhoGDI2 expression in astrocytes after an excitotoxic lesion in the mouse hippocampus
    Min Hee Yi
    Department of Anatomy, Brain Research Institute, Chungnam National University School of Medicine, Daejeon, 301 747, South Korea
    Cell Mol Neurobiol 35:167-74. 2015
    The Rho GDP-dissociation inhibitor (RhoGDI) originally downregulates Rho family GTPases by preventing nucleotide exchange and membrane association...
  69. pmc RhoGDI deficiency induces constitutive activation of Rho GTPases and COX-2 pathways in association with breast cancer progression
    William P Bozza
    Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA
    Oncotarget 6:32723-36. 2015
    Rho GDP Dissociation Inhibitor (RhoGDI) is a key regulator of Rho GTPases. Here we report that loss of RhoGDI significantly accelerated xenograft tumor growth of MDA-MB-231 cells in animal models...
  70. pmc An adventitious interaction of filamin A with RhoGDI2(Tyr153Glu)
    Mia Song
    Hematology Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston MA, USA
    Biochem Biophys Res Commun 469:659-64. 2016
    ..A RhoGDI2 truncate with 81 N-terminal amino acid residues and a phosphomimetic mutant, RhoGDI(Tyr153Glu) interacted with the FLNA construct...
  71. ncbi Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells
    Sandra Schmieder
    Department of Cellular and Molecular Medicine, George Palade Laboratories Room 210, 9500 Gilman Drive, La Jolla, CA 92093 0651, USA
    J Am Soc Nephrol 15:2289-98. 2004
    ..By immunofluorescence redistribution of RhoA and RhoGDI was observed in the presence of both full-length PC and the PC mutant...
  72. ncbi Expression profiling of the estrogen responsive genes in response to phytoestrogens using a customized DNA microarray
    Ryota Ise
    InfoGenes Co, Ltd, Tsukuba, Ibaraki 305 0047, Japan
    FEBS Lett 579:1732-40. 2005
    ....
  73. pmc Identification of a novel prenyl and palmitoyl modification at the CaaX motif of Cdc42 that regulates RhoGDI binding
    Akiyuki Nishimura
    Department of Molecular Medicine, Cornell University College of Veterinary Medicine, Ithaca, New York, USA
    Mol Cell Biol 33:1417-29. 2013
    ..Prenylated and palmitoylated brain Cdc42 did not interact with RhoGDIα and was enriched in the plasma membrane relative to the classically processed form...
  74. pmc 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins
    Philippe Riou
    Randall Division of Cell and Molecular Biophysics, New Hunt s House, King s College London, London, UK
    Cell 153:640-53. 2013
    ..Several Rho GTPases are also regulated by RhoGDI binding and sequestering in the cytosol...
  75. pmc IκB kinase γ/nuclear factor-κB-essential modulator (IKKγ/NEMO) facilitates RhoA GTPase activation, which, in turn, activates Rho-associated KINASE (ROCK) to phosphorylate IKKβ in response to transforming growth factor (TGF)-β1
    Hee Jun Kim
    From the Department of Biochemistry, Hallym University College of Medicine, Chuncheon, Kangwon Do 200 702, Korea
    J Biol Chem 289:1429-40. 2014
    ..We observed that RhoA-GTP level was increased by TGF-β1 in RAW264.7 cells. RhoA-GDP and RhoGDI were bound to N- and C-terminal domains of IKKγ, respectively...
  76. doi Comparative proteomic analysis of anti-cancer mechanism by periplocin treatment in lung cancer cells
    Zejun Lu
    Department of Radiation Oncology, Naval General Hospital of PLA, Beijing, China
    Cell Physiol Biochem 33:859-68. 2014
    ..But the biological processes and molecular pathways by which periplocin induces these beneficial effects remain largely undefined...
  77. ncbi Identification of a novel protein with GDP dissociation inhibitor activity for the ras-like proteins CDC42Hs and rac I
    C N Adra
    Division of Hematology Oncology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215
    Genes Chromosomes Cancer 8:253-61. 1993
    We have recently cloned the human cDNA for a gene, denoted D4, that encodes a protein 67% identical to the bovine rhoGDI protein, a GDP dissociation inhibitor (GDI) for the ras-related rho-subtype proteins...
  78. pmc The complex of Arl2-GTP and PDE delta: from structure to function
    Michael Hanzal-Bayer
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Otto Hahn Strasse 11, D 44227 Dortmund, Germany
    EMBO J 21:2095-106. 2002
    ..PDE delta is structurally closely related to RhoGDI and contains a deep empty hydrophobic pocket...
  79. ncbi Tumor necrosis factor alpha stimulation of Rac1 activity. Role of isoprenylcysteine carboxylmethyltransferase
    Christopher Papaharalambus
    Department of Medicine, Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 280:18790-6. 2005
    ..of ICMT inhibition on the interaction of Rac1 with its inhibitor, Rho guanine nucleotide dissociation inhibitor (RhoGDI)...
  80. pmc Rac-ing to the plasma membrane: the long and complex work commute of Rac1 during cell signaling
    Xose R Bustelo
    Centro de Investigacion del Cancer, Instituto de Biologia Molecular y Celular del Cancer, CSIC University of Salamanca, Campus Unamuno, Salamanca, Spain
    Small Gtpases 3:60-6. 2012
    ..localizations, activation by GDP/GTP exchange, inactivation by GTP hydrolysis, and re-formation of cytosolic Rac1/RhoGDI inhibitory complexes. Here, we summarize the current knowledge about the regulation of those steps...
  81. pmc Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion
    Xihua Lin
    Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China
    Oncotarget 7:19483-98. 2016
    ..PCBP2 may facilitate miR-151-5p and miR-16 promotion of glioma cell migration and invasion through mitigating the function of ARHGDIA. ..
  82. pmc BNN27, a 17-Spiroepoxy Steroid Derivative, Interacts With and Activates p75 Neurotrophin Receptor, Rescuing Cerebellar Granule Neurons from Apoptosis
    Iosif Pediaditakis
    Department of Pharmacology, School of Medicine, University of CreteHeraklion, Greece Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology HellasHeraklion, Greece
    Front Pharmacol 7:512. 2016
    ..the recruitment of p75NTR receptor to its effector protein RIP2 and the simultaneous release of RhoGDI in primary neuronal cells...
  83. ncbi Integrin regulation of membrane domain trafficking and Rac targeting
    A Grande-García
    Centro Nacional de Investigaciones Cardiovasculares CNIC, Ronda de Poniente, 5, 28760 Tres Cantos, Madrid, Spain
    Biochem Soc Trans 33:609-13. 2005
    ..This step is essential for Rac binding to effectors. Integrins increase membrane affinity for Rac, leading to RhoGDI dissociation and effector coupling locally, in the vicinity of activated/bound integrins...
  84. ncbi Mechanisms of statin-mediated inhibition of small G-protein function
    Andrew Cordle
    Department of Neuroscience, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Biol Chem 280:34202-9. 2005
    ..disrupted the interaction of Rac with its negative regulator the Rho guanine nucleotide dissociation inhibitor (RhoGDI), an interaction that is dependent on protein isoprenylation...
  85. ncbi Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro
    Henry E Pelish
    Department of Cell Biology and The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Chem Biol 2:39-46. 2006
    ..Cdc42, a protein involved in membrane traffic, by a mechanism dependent upon the guanine dissociation inhibitor RhoGDI. RhoGDI binds Cdc42 and antagonizes its membrane association, nucleotide exchange and effector binding...
  86. ncbi RhoGDI-1 modulation of the activity of monomeric RhoGTPase RhoA regulates endothelial barrier function in mouse lungs
    Matvey Gorovoy
    University of Illinois College of Medicine, Department of Pharmacology, 835 S Wolcott Ave, Chicago, IL 60612, USA
    Circ Res 101:50-8. 2007
    ..In the present study, we addressed the in vivo role of RhoGDI-1 in regulating pulmonary microvascular permeability using RhoGDI-1(-/-) mice...
  87. ncbi Stimulus-dependent regulation of the phagocyte NADPH oxidase by a VAV1, Rac1, and PAK1 signaling axis
    Kirstine Roepstorff
    Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, 2200N Copenhagen, Denmark
    J Biol Chem 283:7983-93. 2008
    ..In contrast PAK1 substrates LIMK-1 or RhoGDI are not likely to contribute to the PAK1 effect on NADPH oxidase activation...
  88. pmc Synergistic roles of amelogenin and ameloblastin
    J Hatakeyama
    Functional Genomics Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Dent Res 88:318-22. 2009
    ..Proteomic analysis of the double-null teeth revealed increased levels of RhoGDI (Arhgdia), a Rho-family-specific guanine nucleotide dissociation inhibitor, which is involved in important ..
  89. pmc Morphological and proliferative abnormalities in renal mesangial cells lacking RhoGDI
    Heike Bielek
    Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, United States
    Cell Signal 21:1974-83. 2009
    ..Rho GTPase activities and cytosol-membrane cycling are regulated by Rho GDP Dissociation Inhibitor (RhoGDI), and RhoGDI knockout mice develop defects in kidney structure and function that lead to death due to renal ..
  90. doi Functional analysis of RhoGDI inhibitory activity on vacuole membrane fusion
    Michael R Logan
    Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Biochem J 434:445-57. 2011
    ..We have shown previously that purified yeast RhoGDI Rdi1p, can inhibit vacuole membrane fusion in vitro...
  91. doi Rho kinase-related proteins in human vaginal arteries: an immunohistochemical and functional study
    Stefan Uckert
    Hannover Medical School, Division of Surgery, Dept of Urology and Urological Oncology, Hannover, Germany
    J Sex Med 8:2739-45. 2011
    ..Until now, it is not known whether ROKs and related proteins play a role in the control of vaginal blood flow...
  92. doi Cell migration: PKA and RhoA set the pace
    Karen A Newell-Litwa
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Curr Biol 21:R596-8. 2011
    ..PKA activity near the leading edge of protrusions phosphorylates RhoA and inhibits its activity via increased association with RhoGDI.
  93. pmc ARHGDIA: a novel gene implicated in nephrotic syndrome
    Indra Rani Gupta
    Department of Pediatrics, Division of Nephrology, Montreal Children s Hospital and McGill University, 2300, rue Tupper E222, Montreal, Quebec, Canada H3H 1P3
    J Med Genet 50:330-8. 2013
    ..While most cases are due to mutations in one of five genes, in up to 15% of cases, a genetic cause is not identified. We investigated two sisters with a presumed recessive form of congenital nephrotic syndrome...
  94. pmc Role of the Rho GTPase Rac in the activation of the phagocyte NADPH oxidase: outsourcing a key task
    Edgar Pick
    Julius Friedrich Cohnheim Laboratory of Phagocyte Research Department of Clinical Microbiology and Immunology Sackler School of Medicine Tel Aviv University Tel Aviv, Israel
    Small Gtpases 5:e27952. 2014
    ..p22(phox)) and 4 cytosolic components: p47(phox), p67(phox), p40(phox), and the small GTPase, Rac, in complex with RhoGDI. Superoxide is produced by the NADPH-driven reduction of molecular oxygen, via a redox gradient located in Nox2...
  95. doi Correlation of the Rac1/RhoA pathway with ezrin expression in osteosarcoma
    Caterina Chiappetta
    Department of Medical Surgical Sciences and Biotechnologies, Pathology Unit, ICOT, Sapienza University of Rome, Latina, Italy
    Appl Immunohistochem Mol Morphol 22:162-70. 2014
    ..Ezrin interacts with RhoGDI by dissociating it from RhoGTPases, which allow GTPases to load with GTP, activate RhoA to increase cell migration,..
  96. doi RhoGDI facilitates geranylgeranyltransferase-I-mediated RhoA prenylation
    Zakir Tnimov
    Department of Molecular Cell Biology, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St Lucia, QLD 4072, Australia
    Biochem Biophys Res Commun 452:967-73. 2014
    ..One such carrier is Rho GDP dissociation inhibitor (RhoGDI)...
  97. doi Loss of Rho-GDIα sensitizes podocytes to lipopolysaccharide-mediated injury
    Richard Robins
    Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada and
    Am J Physiol Renal Physiol 308:F1207-16. 2015
    ....
  98. pmc Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants
    Reshu Saxena
    Toxicology Division, CSIR Central Drug Research Institute, Sector 10, Janakipuram Extension, Sitapur Road, Lucknow, India
    PLoS ONE 10:e0122994. 2015
    ..Downregulation of α-enolase (ENO1), VDAC1 and OTUB1 was more significant by Nef RP01 whereas Cyclophilin A and RhoGDI were found to be more downregulated by Nef RP14...
  99. pmc Dual regulation by microRNA-200b-3p and microRNA-200b-5p in the inhibition of epithelial-to-mesenchymal transition in triple-negative breast cancer
    Lyndsay V Rhodes
    Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, FL, USA
    Oncotarget 6:16638-52. 2015
    ..In addition, a compensatory mechanism for miR-200b-3p/200b-5p targeting, where both miRNAs target the RHOGDI pathway leading to non-canonical repression of EMT, was demonstrated...
  100. doi Sustainable inflammation transforms hepatic cells by causing oxidative stress injury and potential epithelial-mesenchymal transition
    Kun Lu
    Department of Basic Medicine, Medical College of Xiamen University Cancer Research Center of Xiamen University, Xiamen, Fujian 361102, P R China
    Int J Oncol 49:971-80. 2016
    ..signaling, signaling by Rho family GTPases, IL-8 signaling, and ILK signaling, as well as the inhibition of RhoGDI signaling...
  101. pmc Immunological and Functional Characterization of RhoGDI3 and Its Molecular Targets RhoG and RhoB in Human Pancreatic Cancerous and Normal Cells
    Mercedes Piedad de León-Bautista
    Departamento de Biomedicina Molecular, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Avenida Instituto Politecnico Nacional 2508, Col San Pedro Zacatenco, C P 07360, Mexico City, Mexico
    PLoS ONE 11:e0166370. 2016
    b>RhoGDI proteins have been implicated in several human cancers; changes in their expression levels have shown pro- or anti-tumorigenic effects...

Research Grants18

  1. Celine DerMardirossian; Fiscal Year: 2016
    ..These studies will lead to a greater understanding of how these important components of renal function are controlled, ultimately guiding to unique and increasingly selective therapeutic approaches to intervene in nephritic disorders. ..
  2. Alexander Staruschenko; Fiscal Year: 2016
    ..role of this pathway in the development of salt-sensitive hypertension;2) To establish the physiological role of RhoGDI and Rac1 in regulation of ENaC and determine the role of Rac1 in mediating EGF effects on ENaC;and 3) To define ..
  3. Role of Rho GDP dissociation inhibitors in androgen signaling in prostate cancer
    Allen C Gao; Fiscal Year: 2013
    ..androgen signaling in prostate cancer KEYWORDS (MeSH terms only;minimum three) Prostate cancer, androgen receptor, RhoGDI, castration-resistance, proliferation BRIEF STATEMENT OF RESEARCH OBJECTIVES (Do not use continuation sheets) ..
  4. Keith Burridge; Fiscal Year: 2016
    ..In the final aim, we will focus on the Rho GTPase sequestering protein RhoGDI, which we have recently found contributes to crosstalk between different Rho family members...
  5. Friedhelm Hildebrandt; Fiscal Year: 2016
    ..SA 3. For newly identified SRNS/SSNS genes study the gene function and therapeutic approaches in zebrafish and mouse models, including Coq6-/-. ..
  6. MECHANISM AND FUNCTION OF PROTEIN PRENYLATION
    Mark D Distefano; Fiscal Year: 2010
    ..in cancer cells treated with inhibitors of protein prenylation;(5) Augment the affinity of peptides that bind to RhoGDI and study the mechanism by which they cause cell death;(6) Study the effect of prenyl group structure on ..
  7. G Proteins and Endothelial Barrier Regulation
    Tatyana Voyno Yasenetskaya; Fiscal Year: 2004
    ..effector in signaling the G-alpha 13-induced Rho activation via the Rho guanine nucleotide dissociation inhibitor, RhoGDI. We will address a new and potentially important signaling pathway for the G-alpha13-dependent Rho activation and ..
  8. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
    ..e. Lys and Glu). Preliminary results, obtained with the human protein RhoGDI (Rho Guanine nucleotide Dissociation Inhibitor), the LH domain from PDZ-RhoGEF, and the BH domain from GRAF, show ..
  9. REGULATION OF NEUTROPHIL FUNCTION
    J Lambeth; Fiscal Year: 2000
    ..plasma membrane and from the cytosol using a combination of immunoprecipitation and extraction with recombinant RhoGDI (GDP Dissociation Inhibitor)...
  10. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Celine DerMardirossian; Fiscal Year: 2010
    ..We will study how a newly identified group of Src target proteins act to organize Nox assembly and localized activity. Understanding Nox regulation will have important implications for disease therapy. ..
  11. delta-Catenin and Cell-Cell Adhesion in Prostate Cancer
    Qun Lu; Fiscal Year: 2010
    ..Understanding the posttranslational modifications that control 8-catenin functions will unravel mechanisms by which S-catenin modulates gene expression and promotes CaP in vivo. ..
  12. Dynamic Analysis of Rho GTPase-Rho GDI Cycling
    Leslie M Loew; Fiscal Year: 2010
    A key regulator of both the Rho GTPase GDP/GTP activity cycle and the cytosol/membrane localization cycle is RhoGDI. Our current understanding of RhoGDI regulation and the dynamics of its interactions with Rho GTPases during in vivo cell ..
  13. Allosteric, Small-Molecule Inhibitors of Actin Nucleation by the Formin mDia1
    Jeffrey Peterson; Fiscal Year: 2008
    ..Future work will apply this screen to a much larger compound collection and we envisage applying a similar strategy to develop inhibitors for other formins. [unreadable] [unreadable] [unreadable]..
  14. Role of Vav family proteins in cell signaling and cancer
    XOSE BUSTELO; Fiscal Year: 2009
    ..abstract_text> ..
  15. Role of Myelin in Spinal Cord Regeneration
    Marie Filbin; Fiscal Year: 2008
    ..Through these studies a molecular insight will be gained into encouraging axons to regenerate in vivo and possible therapies may be identified. ..
  16. Delta-Catenin Cleavage by Presenilin and Synaptic Remodeling
    Qun Lu; Fiscal Year: 2006
    ..It will also lay the foundation for future R01 investigation of the roles of delta-catenin proteolytic fragments in AD pathogenesis. ..
  17. Myelin Gordon Conference
    Marie Filbin; Fiscal Year: 2002
    ..Each session has 3 or 4 speakers and a discussion leader. ..
  18. Cofilin/ADF Regulation in Rho GTPase Signaling
    Celine DerMardirossian; Fiscal Year: 2010
    ..Our studies will investigate a novel regulator of this important process. In addition, this protein is likely to play important roles in ischemic disorders and other stress responses. ..