Genomes and Genes
Gene Symbol: rev
Alias: p19, Rev
Species: Human immunodeficiency virus 1
Publications130 found, 100 shown here
- Nuclear RNA export and packaging functions of HIV-1 Rev revisitedMaik Blissenbach
Department of Molecular and Medical Virology, Ruhr University Bochum, Universitaetsstr 150, D 44780 Bochum, Germany
J Virol 84:6598-604. 2010Although the viral Rev protein is necessary for HIV replication, its main function in the viral replication cycle has been controversial...
- Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAsVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
Proc Natl Acad Sci U S A 107:14787-92. 2010..CRM-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNAs...
- Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expressionVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, Maryland 20892 0460, USA
Retrovirology 8:61. 2011..Specific signals on the viral RNAs, such as instability sequences (INS) and Rev responsive element (RRE), are binding sites for viral and cellular factors that serve to regulate RNA-export...
- Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response elementC Fankhauser
Department of Biochemistry, University of Geneva, Switzerland
Mol Cell Biol 11:2567-75. 1991The human immunodeficiency virus type 1 (HIV) Rev protein is thought to be involved in the export of unspliced or singly spliced viral mRNAs from the nucleus to the cytoplasm...
- Single-molecule studies reveal that DEAD box protein DDX1 promotes oligomerization of HIV-1 Rev on the Rev response elementRae M Robertson-Anderson
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Mol Biol 410:959-71. 2011Oligomeric assembly of Rev on the Rev response element (RRE) is essential for the nuclear export of unspliced and singly spliced human immunodeficiency virus type 1 viral mRNA transcripts...
- Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1H P Bogerd
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Virol 72:8627-35. 1998The hypothesis that the cellular protein Crm1 mediates human immunodeficiency virus type 1 (HIV-1) Rev-dependent nuclear export posits that Crm1 can directly interact both with the Rev nuclear export signal (NES) and with cellular ..
- The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particlesAdam S Cockrell
Gene Therapy Center University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
Retrovirology 8:51. 2011..Although conventionally associated with nuclear export of HIV-1 RNA, there is a burgeoning role for the Rev/RRE in the encapsidation process...
- Rev-dependent association of the intron-containing HIV-1 gag mRNA with the nuclear actin bundles and the inhibition of its nucleocytoplasmic transport by latrunculin-BT Kimura
Departments of Microbiology and Physiology, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
Genes Cells 5:289-307. 2000A hallmark of HIV-1 gene expression is that unspliced genomic RNA, which also acts as mRNA for the expression of Gag/Pol, is exported to the cytoplasm. Rev directs this transport through the nuclear export signal (NES).
- The cellular HIV-1 Rev cofactor hRIP is required for viral replicationZhong Yu
Program in Molecular Medicine and Center for AIDS Research CFAR, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 102:4027-32. 2005..The HIV-1 Rev protein facilitates the cytoplasmic accumulation of the intron-containing viral gag-pol and env mRNAs and is ..
- Rev-ing up post-transcriptional HIV-1 RNA expressionVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, MD, USA
RNA Biol 8:195-9. 2011..HIV-1 has evolved a protein, Rev, that participates in the export of unspliced / partially spliced viral RNAs from the nucleus...
- HIV-1 Rev transactivator: a beta-subunit directed substrate and effector of protein kinase CK2F Meggio
Dipartimento di Chimica Biologica, Centro di Studio delle Biomembrane del CNR, Padova, Italy
Mol Cell Biochem 227:145-51. 2001The phosphorylation of HIV-1 Rev by protein kinase CK2 is strictly dependent on the regulatory beta subunit of the kinase and is deeply affected by conformational changes of the substrate outside the phosphorylation site...
- Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA)Ruonan Zhang
Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America
PLoS ONE 5:e15620. 2010..pore complex and has been implicated in various nucleo-cytoplasmic transport pathways including those for HIV Rev-protein. We sought to investigate the role of RanBP2 in HIV-1 replication...
- Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNAKim Marsh
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
Retrovirology 5:97. 2008....
- A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleusF Stutz
Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
Mol Cell Biol 16:7144-50. 1996The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae...
- In vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32T O Tange
Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, DK 8000 Aarhus C, Denmark
J Biol Chem 271:10066-72. 1996Continuous replication of human immunodeficiency virus type 1 requires the expression of the regulatory protein Rev, which binds to the Rev response element (RRE) and up-regulates the cytoplasmic appearance of singly spliced and ..
- Functional domains of the HIV-1 rev gene required for trans-regulation and subcellular localizationL K Venkatesh
Institute for Molecular Virology, St Louis University School of Medicine, Missouri 63110
Virology 176:39-47. 1990The rev gene of human immunodeficiency virus type 1 (HIV-1) encodes a 116 amino acid nuclear regulatory protein (Rev) that increases the cytoplasmic expression of viral mRNAs containing the Rev response element (RRE) and coding for the ..
- Binding of human prothymosin alpha to the leucine-motif/activation domains of HTLV-I Rex and HIV-1 RevS Kubota
Laboratory of Molecular Virology and Carcinogenesis, NCI Frederick Cancer Research and Development Center, MD 21702, USA
Eur J Biochem 233:48-54. 1995Rex of human T-cell leukemia virus type I (HTLV-I) and Rev of human immunodeficiency virus 1 (HIV-1) are post-transcriptional regulators of viral gene expression...
- Distinct domains of IkappaB-alpha inhibit human immunodeficiency virus type 1 replication through NF-kappaB and RevB Y Wu
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
J Virol 71:3161-7. 1997..HIV-1) replication, and unexpectedly, IkappaB-alpha was found both to suppress HIV-1 transcription and to inhibit Rev function. The relative contributions and specificities of these mechanisms to HIV replication were unknown...
- High frequency of defective vpu compared with tat and rev genes in brain from patients with HIV type 1-associated dementiaElaine R Thomas
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
AIDS Res Hum Retroviruses 23:575-80. 2007..Here we analyzed sequences of tat, rev, and vpu genes in 55 subgenomic clones previously shown to encode functional env genes from brain and lymphoid ..
- Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAsRui Yi
Department of Microbiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Virol 76:2036-42. 2002..that encode the late, structural proteins of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev regulatory protein...
- The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAsU Fischer
Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
Cell 82:475-83. 1995HIV-1 Rev protein directs nuclear export of pre-mRNAs and mRNAs containing its binding site, the Rev response element (RRE)...
- Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export functionSilvio Urcuqui-Inchima
Grupo de Inmunovirología, Corporación Biogénesis, Universidad de Antioquia, Medellin, Colombia
Retrovirology 3:83. 2006The HIV Rev protein is known to facilitate export of incompletely spliced and unspliced viral transcripts to the cytoplasm, a necessary step in virus life cycle...
- Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutantsA M Szilvay
National Centre for Research in Virology, Gade Institute, University of Bergen, Norway
J Virol 69:3315-23. 1995The human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev moves repeatedly between the cytoplasm, a perinuclear zone, the nucleoli, and nucleoplasmic speckles...
- Direct participation of Sam68, the 68-kilodalton Src-associated protein in mitosis, in the CRM1-mediated Rev nuclear export pathwayJinliang Li
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA
J Virol 76:8374-82. 2002..efficient nuclear export of incompletely spliced and unspliced HIV-1 mRNA transcripts, which is achieved by Rev expression at an early stage of the viral life cycle...
- Interaction of nucleolar protein B23 with peptides related to nuclear localization signalsA Szebeni
Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216 4505, USA
Biochemistry 34:8037-42. 1995..A peptide containing the sequence required for nucleolar localization of the HIV-1 Rev protein had an affinity for B23 approximately 10-fold greater than that of the SV40 T-NLS...
- The road to chromatin - nuclear entry of retrovirusesYouichi Suzuki
Laboratory for Host Factors, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin kawara cho, Sakyo ku, Kyoto 606 8507, Japan
Nat Rev Microbiol 5:187-96. 2007..Before integration into the host genome can occur, this complex must be transported to the nucleus and must cross the nuclear envelope. This Review summarizes our current knowledge of how this journey is accomplished...
- Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteomeCheryl Bolinger
Center for Retrovirus Research, Department of Veterinary Biosciences, Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH, USA
Retrovirology 6:8. 2009..By contrast, nuclear protein hnRNP E1 negatively modulates HIV-1 Gag, Env, and Rev protein synthesis...
- The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev proteinM Dundr
Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216, USA
J Cell Sci 108:2811-23. 1995The human immunodeficiency virus 1 (HIV-1) Rev transactivator protein plays a critical role in the regulation of expression of structural proteins by controlling the pathway of mRNA transport...
- HIV-1 Rev is capable of shuttling between the nucleus and cytoplasmN Richard
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
Virology 204:123-31. 1994The mechanism by which Rev facilitates the export, and consequently, the translation of the structural protein mRNAs of the human immunodeficiency virus type 1 remains undefined...
- The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assemblyJ Kjems
Department of Molecular Biology, University of Aarhus, Denmark
J Virol 67:4769-76. 1993Human immunodeficiency virus type 1 (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA...
- Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathwayR A Fridell
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 93:4421-4. 1996The Rev protein of HIV-1 is essential for the nuclear export of incompletely spliced viral mRNAs...
- Specific binding of polypyrimidine tract binding protein and hnRNP A1 to HIV-1 CRS elementsA C Black
Virus Genes 12:275-85. 1996The human immunodeficiency virus (HIV) Rev and human T-cell leukemia virus (HTLV) Rex proteins regulate viral RNA processing...
- Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1A S Zolotukhin
Human Retrovirus Pathogenesis Group, NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, Frederick, Maryland 21702 1201, USA
J Biol Chem 272:11356-60. 1997..is responsible for the cytoplasmic accumulation of RanBP1 and can functionally replace the nuclear export signal of Rev of human immunodeficiency virus type 1...
- A role for Rev in the association of HIV-1 gag mRNA with cytoskeletal beta-actin and viral protein expressionT Kimura
Department of Microbiology, Kansai Medical University, Osaka, Japan
Biochimie 78:1075-80. 1996Human immunodeficiency virus type-1 (HIV-1) Rev acts by inducing the specific nucleocytoplasmic transport of a class of incompletely spliced RNAs that encodes the viral structural proteins...
- Eukaryotic initiation factor 5A activity and HIV-1 Rev functionD Bevec
Novartis Research Institute, Department of Immunodermatology, Vienna, Austria
Biol Signals 6:124-33. 1997..The unexpected finding that eIF-5A is a cellular cofactor of the HIV-1 Rev trans-activator protein may, however, provide a novel opportunity to reveal precisely what function eIF-5A performs ..
- Interaction of eukaryotic initiation factor 5A with the human immunodeficiency virus type 1 Rev response element RNA and U6 snRNA requires deoxyhypusine or hypusine modificationY P Liu
Department of Chemistry, Rutgers State University of New Jersey 08855 0939, USA
Biol Signals 6:166-74. 1997..Recent studies have suggested that eIF-5A may be the cellular target of the human immunodeficiency virus type 1 Rev and human T cell leukemia virus type 1 Rex proteins...
- Signal peptide fragments of preprolactin and HIV-1 p-gp160 interact with calmodulinB Martoglio
Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Postfach 106249, 69052 Heidelberg, Germany
EMBO J 16:6636-45. 1997..The functional consequences of such interactions remain to be established. However, our data suggest that signal sequences may be functionally more versatile than anticipated up to now...
- Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 RevT R Reddy
Department of Medicine, University of California San Diego, La Jolla 92093, USA
Nat Med 5:635-42. 1999The HIV-1 Rev protein facilitates the nuclear export of mRNA containing the Rev response element (RRE) through binding to the export receptor CRM-1...
- Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1O Rosorius
Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Schlossgarten 4, D 91054 Erlangen, Germany
J Cell Sci 112:2369-80. 1999..The finding that eIF-5A is an essential cofactor of the human immunodeficiency virus type 1 (HIV-1) Rev RNA transport factor suggested that eIF-5A is part of a specific nuclear export pathway...
- HIV-1 rev depolymerizes microtubules to form stable bilayered ringsN R Watts
Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD, USA
J Cell Biol 150:349-60. 2000We describe a novel interaction between HIV-1 Rev and microtubules (MTs) that results in the formation of bilayered rings that are 44-49 nm in external diameter, 3.4-4.2 MD (megadaltons) in mass, and have 28-, 30-, or 32-fold symmetry...
- Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAsDusan Cmarko
Centre of Electron Microscopy, University of Lausanne, 27 Bugnon, CH 1005 Lausanne, Switzerland
J Virol 76:10473-84. 2002..RNA maturation pathways, we analyzed the intracellular distribution of HIV-1 RNA and the viral regulatory proteins Rev and Tat in transfected COS cells and HIV-1-infected lymphoid C8166 cells by means of ultrastructural in situ ..
- hnRNP A1 controls HIV-1 mRNA splicing through cooperative binding to intron and exon splicing silencers in the context of a conserved secondary structureChristian Kroun Damgaard
Department of Molecular and Structural Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
RNA 8:1401-15. 2002The removal of the second intron in the HIV-1 rev/tat pre-mRNAs, which involves the joining of splice site SD4 to SA7, is inhibited by hnRNP A1 by a mechanism that requires the intronic splicing silencer (ISS) and the exon splicing ..
- hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear regionNuria Sánchez-Velar
University of Massachusetts Medical School, Program in Molecular Medicine and the UMASS Center for AIDS Research CFAR, Worcester, Massachusetts 01605, USA
Genes Dev 18:23-34. 2004Human immunodeficiency virus Rev facilitates the cytoplasmic accumulation of viral RNAs that contain a Rev binding site...
- Regulation of HIV-1 env mRNA translation by Rev proteinCelia Perales
Centro de Biologia Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
Biochim Biophys Acta 1743:169-75. 2005We have examined the effect of Rev on the regulation of the expression of RRE containing mRNAs when they were synthesised in the nucleus or directly in the cytoplasm...
- Identification of a novel Rev-interacting cellular proteinSusanne Kramer-Hammerle
Institute of Molecular Virology, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
BMC Cell Biol 6:20. 2005..The HIV Rev protein is a post-transcriptional inducer of HIV gene expression and an important target for interaction with ..
- Functional analysis of backbone cyclic peptides bearing the arm domain of the HIV-1 Rev protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expressionElana Hariton-Gazal
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9104, Israel
Biochemistry 44:11555-66. 2005..the synthesis and activity of a novel backbone cyclic (BC) peptide library based on the sequence of the HIV-1 Rev arginine-rich motif (ARM)...
- RTE and CTE mRNA export elements synergistically increase expression of unstable, Rev-dependent HIV and SIV mRNAsSergey Smulevitch
Human Retrovirus Pathogenesis Section, National Cancer Institute Frederick, MD 21702 1201, USA
Retrovirology 3:6. 2006..RTE and CTE are potent in nucleocytoplasmic mRNA transport and expression, neither element is as powerful as the Rev-RRE posttranscriptional control...
- Inhibition of multidrug-resistant HIV-1 by interference with cellular S-adenosylmethionine decarboxylase activityBirgit Schäfer
Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse, Hamburg, Germany
J Infect Dis 194:740-50. 2006..which is a cellular cofactor of the essential human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev. In the present study, we show that SAM486A efficiently suppresses HIV-1 replication, including the replication of ..
- Evidence for CTL-mediated selection of Tat and Rev mutants after the onset of the asymptomatic period during HIV type 1 infectionC Guillon
CNRS, FRE2736 CNRS bioMérieux, IFR128 Biosciences Lyon Gerland, Tour CERVI, 69007 Lyon, France
AIDS Res Hum Retroviruses 22:1283-92. 2006..We investigated the contribution of both selection processes to the overall evolution of the Tat and Rev regulatory gene sequences from four individuals, ranging in time from just prior to seroconversion to stable ..
- Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteinsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Mol Med 16:34-44. 2010The present work describes a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Rev protein and the cellular lens epithelium-derived growth factor p75 (LEDGF/p75) protein in vitro and in virus-infected cells...
- Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
Virus Genes 40:341-6. 2010Expression of the human immunodeficiency virus type 1 (HIV-1) Rev protein is essential for completion of the viral life cycle...
- The cytotoxicity of human immunodeficiency virus type 1 Rev: implications for its interaction with the nucleolar protein B23Y Miyazaki
Institute for Virus Research, Kyoto University, Japan
Exp Cell Res 219:93-101. 1995..type 1 (HIV-1) encodes several regulatory proteins, including two essential trans-activators for viral replication, Rev and Tat. Both Rev and Tat have a nucleolar targeting signal and are actually located predominantly in the nucleoli...
- Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteinsH P Bogerd
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
Cell 82:485-94. 1995HIV-1 Rev is the prototype of a class of retroviral regulatory proteins that induce the sequence-specific nuclear export of target RNAs...
- A human nucleoporin-like protein that specifically interacts with HIV RevC C Fritz
Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605, USA
Nature 376:530-3. 1995The Rev protein of human immunodeficiency virus type 1 (HIV-1) facilitates the nuclear export of unspliced and partly spliced viral RNAs...
- The human immunodeficiency virus type 1 Rev protein shuttles between the cytoplasm and nuclear compartmentsK H Kalland
Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Centre, University of Bergen, Norway
Mol Cell Biol 14:7436-44. 1994A retroviral regulatory protein, Rev (regulator of virion protein expression), is made in cells infected by human immunodeficiency virus (HIV)...
- The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasmB E Meyer
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
Genes Dev 8:1538-47. 1994The HIV-1 Rev protein is a nuclear trans-activator essential for the transport of unspliced viral transcripts to the cytoplasm...
- Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activationM Ruhl
Sandoz Research Institute, Vienna, Austria
J Cell Biol 123:1309-20. 1993..virus type 1 (HIV-1) structural proteins requires the presence of the viral trans-activator protein Rev. Rev is localized in the nucleus and binds specifically to the Rev response element (RRE) sequence in viral RNA...
- Role of polyadenylation in nucleocytoplasmic transport of mRNAY Huang
Department of Microbiology, University of Connecticut Health Center, Farmington, 06030, USA
Mol Cell Biol 16:1534-42. 1996To examine the role of polyadenylation in the nuclear export of mRNA, we have replaced the poly(A) signal in a Rev-responsive human immunodeficiency virus type 1-based reporter gene with a cis-acting hammerhead ribozyme...
- Phosphorylation of HIV-1 Rev protein: implication of protein kinase CK2 and pro-directed kinasesF Meggio
Dipartimento di Chimica Biologica, Universita di Padova, Italy
Biochem Biophys Res Commun 226:547-54. 1996HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase...
- Nucleolar protein B23 stimulates nuclear import of the HIV-1 Rev protein and NLS-conjugated albuminA Szebeni
Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216, USA
Biochemistry 36:3941-9. 1997..1994) J. Biol. Chem. 269, 4910-4916] using NLS peptide-conjugated bovine serum albumin (NLS-BSA) or the HIV-1 Rev protein as substrates for import into isolated rat liver nuclei...
- The post-transcriptional regulator Rev of HIV: implications for its interaction with the nucleolar protein B23Y Miyazaki
Institute for Virus Research, Kyoto University, Japan
Biochimie 78:1081-6. 1996..HIV-1 encodes several regulatory proteins, including two essential trans-activations for viral replication, Rev and Tat. Both Rev and Tat have a nucleolar targeting signal and are actually located predominantly in the nucleoli...
- Site-specific phosphorylation of the human immunodeficiency virus type-1 Rev protein accelerates formation of an efficient RNA-binding conformationD E Fouts
Department of Microbiology and College of Medicine, University of Illinois at Urbana Champaign, USA
Biochemistry 36:13256-62. 1997..In this report, we demonstrate that phosphorylation of a recombinant human immunodeficiency virus type-1 Rev protein promotes rapid formation of an efficient RNA-binding state...
- The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear exportM Neville
Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
Curr Biol 7:767-75. 1997The human immunodeficiency virus (HIV-1) uses the viral protein Rev to regulate gene expression by promoting the export of unspliced and partially spliced viral transcripts...
- Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-betaB R Henderson
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England
J Mol Biol 274:693-707. 1997The human immunodeficiency virus type 1 (HIV-1) Rev protein binds to unspliced HIV-1 pre-mRNA and exports it from the nucleus. Rev itself can "shuttle" between the nucleus and cytoplasm...
- Subcellular localization of human immunodeficiency virus type 1 RNAs, Rev, and the splicing factor SC-35S O Bøe
Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Center, University of Bergen, Norway
Virology 244:473-82. 1998The HIV-1 protein Rev regulates the cytoplasmic levels of incompletely spliced HIV-1 mRNAs. The plasmid pSVc21, which contains a HIV-1 provirus, was introduced into COS cells by transient transfection...
- The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTPP Askjaer
Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
J Biol Chem 273:33414-22. 1998Nuclear export of intron-containing human immunodeficiency virus type 1 (HIV-1) RNA is mediated by the viral Rev protein that contains both an RNA binding domain specific for the viral Rev response element (RRE) and a nuclear export ..
- Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 RevA S Zolotukhin
Human Retrovirus Pathogenesis Group, ABL Basic Research Program, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
J Virol 73:120-7. 1999Human immunodeficiency virus type 1 (HIV-1) Rev contains a leucine-rich nuclear export signal that is essential for its nucleocytoplasmic export mediated by hCRM1...
- The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin beta-dependent nuclear localization signalsR Truant
Howard Hughes Medical Institute and Department of Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cell Biol 19:1210-7. 1999..that the arginine-rich NLS sequences present in the human immunodeficiency virus type 1 regulatory proteins Tat and Rev fail to interact with Imp alpha and instead bind directly to Imp beta...
- A role for RNA helicase A in post-transcriptional regulation of HIV type 1J Li
Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 96:709-14. 1999..by a cis-acting constitutive transport element (CTE) for simple retroviruses and by the trans-acting viral protein Rev in concert with its response element (RRE) for complex retroviruses (e.g., HIV)...
- Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1I Najera
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
J Mol Biol 285:1951-64. 1999..Efficient transport of these mRNAs from the nucleus requires the binding of the viral nuclear transport protein Rev to an RNA stem-loop structure called the RRE (Rev response element)...
- The nucleoporin nup153 plays a critical role in multiple types of nuclear exportK S Ullman
Department of Biology, University of California at San Diego, La Jolla, California 92093 0347, USA
Mol Biol Cell 10:649-64. 1999..Nup153 antibodies also block the NES protein export pathway, specifically the export of the HIV Rev protein, as well as Rev-dependent RNA export...
- Regulation of human immunodeficiency virus type 1 infectivity by the ERK mitogen-activated protein kinase signaling pathwayX Yang
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Virol 73:3460-6. 1999..The HIV-1 Rev, Tat, p17(Gag), and Nef proteins were directly phosphorylated by MAPK in vitro, suggesting that other HIV-1 ..
- A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1G Farjot
Laboratoire de Virologie, ENS Lyon, Unité INSERM U 412, 46, Allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 274:17309-17. 1999The HIV-1 Rev is a shuttling protein required for the nuclear export of unspliced and partially spliced viral mRNA...
- Rev-mediated nuclear export of RNA is dominant over nuclear retention and is coupled to the Ran-GTPase cycleU Fischer
Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
Nucleic Acids Res 27:4128-34. 1999The human immunodeficiency virus type-1 Rev protein induces the nuclear export of intron-containing viral mRNAs that harbor its binding site, the Rev response element (RRE)...
- Sam68, RNA helicase A and Tap cooperate in the post-transcriptional regulation of human immunodeficiency virus and type D retroviral mRNAT R Reddy
Department of Medicine, University of California, San Diego, La Jolla 92093 0665, USA
Oncogene 19:3570-5. 2000..nuclear export pathways for retroviral mRNA have been described: a CRM-1 dependent pathway mediated by the HIV-1 Rev protein and the Rev Response Element (RRE), and a CRM-1 independent pathway mediated by the Constitutive Transport ..
- General effect of Sam68 on Rev/Rex regulated expression of complex retrovirusesT R Reddy
Department of Medicine, University of California San Diego, La Jolla, California, CA 92093 0665, USA
Oncogene 19:4071-4. 2000..demonstrated that overexpression of Sam68 functionally substitutes for, as well as synergizes with, HIV-1 Rev in RRE-mediated gene expression and virus replication...
- Unique features of HIV-1 Rev protein phosphorylation by protein kinase CK2 ('casein kinase-2')O Marin
Dipartmento di Chimica Biologica and Centro del CNR per lo Studio delle Biomembrane, Università delgi Studi di Padova, Padua, Italy
FEBS Lett 481:63-7. 2000The HIV-1 Rev transactivator is phosphorylated in vitro by protein kinase CK2 at two residues, Ser-5 and Ser-8; these sites are also phosphorylated in vivo...
- Identification of a domain in human immunodeficiency virus type 1 rev that is required for functional activity and modulates association with subnuclear compartments containing splicing factor SC35D M D'Agostino
Dipartimento di Scienze Oncologiche e Chirurgiche, Sezione di Oncologia, University of Padova, Italy
J Virol 74:11899-910. 2000The activity of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a leucine-rich nuclear export ..
- Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actinW Hofmann
Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
J Cell Biol 152:895-910. 2001..Here, we investigate human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export and Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE)-mediated nuclear ..
- Inhibition of human immunodeficiency virus type 1 Rev function by a dominant-negative mutant of Sam68 through sequestration of unspliced RNA at perinuclear bundlesV B Soros
Department of Medical and Molecular Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8
J Virol 75:8203-15. 2001Human immunodeficiency virus (HIV) type 1 encodes an essential protein, Rev, which functions to transport unspliced and singly spliced viral transcripts from the nucleus to the cytoplasm to allow expression of the viral structural ..
- Expression of exogenous Sam68, the 68-kilodalton SRC-associated protein in mitosis, is able to alleviate impaired Rev function in astrocytesJinliang Li
Department of Microbiology and Immunology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
J Virol 76:4526-35. 2002..Impaired Rev function has been documented in these cells...
- The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functionsYoshiyuki Hakata
Institute for Genetic Medicine, Hokkaido University, Japan
J Virol 76:8079-89. 2002The human immunodeficiency virus type 1 (HIV-1) regulatory protein, Rev, mediates the nuclear export of unspliced and singly spliced viral mRNAs by bridging viral RNA and export receptor human CRM1 (hCRM1)...
- A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative bindingVirginie Marchand
Laboratoire de Maturation des ARN et Enzymologie Moleculaire, UMR 7567 UHP CNRS, Universite Henri Poincare Nancy 1, Boulevard des Aiguillettes, BP239, 54506 Cedex, Vandoeuvre les Nancy, France
J Mol Biol 323:629-52. 2002..The HIV-1 acceptor site A7 is required for tat and rev mRNA production...
- A nuclear kinesin-like protein interacts with and stimulates the activity of the leucine-rich nuclear export signal of the human immunodeficiency virus type 1 rev proteinL K Venkatesh
Institute for Molecular Virology, Saint Louis University School of Medicine, Saint Louis, Missouri 63108, USA
J Virol 77:7236-43. 2003The Rev protein of human immunodeficiency virus type 1 (HIV-1) is essential for the nucleocytoplasmic transport of unspliced and partially spliced HIV mRNAs containing the Rev response element (RRE)...
- Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactorsA Kiss
Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
Virology 314:591-600. 2003Human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export of viral RNAs involves the interaction of its leucine-rich nuclear export sequence (NES) with nuclear cofactors...
- Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export functionVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
Cell 119:381-92. 2004..export of intron-containing cellular transcripts is restricted in mammalian cells, HIV-1 has evolved the viral Rev protein to overcome this restriction for viral transcripts...
- Within you, without you: HIV-1 Rev and RNA exportAndrew I Dayton
Center for Biologics Evaluation and Research, Food and Drug Administration, USA
Retrovirology 1:35. 2004..This suggests the ubiquity of RNA helicases in RNA export from the nucleus, and has novel mechanistic implications...
- A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of RevJianhua Fang
Division of Infectious Diseases and Environmental Medicine, Department of Medicine, The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
Virology 330:471-80. 2004HIV-1 Rev escorts unspliced viral mRNAs out of the nucleus of infected cells, which allows formation of infectious HIV-1 virions...
- Sam68 is absolutely required for Rev function and HIV-1 productionSuhasini Modem
Department of Immunology and Microbiology, Wayne State University School of Medicine Detroit, MI 48201, USA
Nucleic Acids Res 33:873-9. 2005Sam68 functionally complements for, as well as synergizes with, HIV-1 Rev in Rev response element (RRE)-mediated gene expression and virus production...
- The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytesJianhua Fang
The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
Virology 336:299-307. 2005..Of note, a low ratio of unspliced versus spliced HIV-1-specific RNA was also investigated, as Rev appeared to act aberrantly in astrocytes, via loss of nuclear and/or nucleolar localization and diminished Rev-..
- Requirement of an additional Sam68 domain for inhibition of human immunodeficiency virus type 1 replication by Sam68 dominant negative mutants lacking the nuclear localization signalJizhong Zhang
Department of Microbiology and Immunology, Indiana University School of Medicine, R2 302, 950 W Walnut St, Indianapolis, IN 46202, United States
Gene 363:67-76. 2005..This process is evolutionally made possible by expression of HIV-1 Rev, one of the three HIV-1 proteins encoded by completely spliced HIV-1 RNAs...
- Modulation of HIV-1 Rev protein abundance and activity by polyubiquitination with unconventional Lys-33 branchingAnne Laure Vitte
Laboratoire de Biologie Moleculaire de la Cellule, UMR5161 CNRS ENS de Lyon, IFR 128 Biosciences Lyon Gerland, 46 allee d Italie, 69364 Lyon Cedex 07, France
FEBS Lett 580:6155-60. 2006The HIV-1 Rev protein plays a key role in virus replication by allowing export to the cytoplasm of unspliced or singly-spliced RNAs. In this report, we investigated whether Rev is modified by ubiquitination or sumoylation...
- PRMT6 diminishes HIV-1 Rev binding to and export of viral RNACédric F Invernizzi
McGill University AIDS Centre, Lady Davis Institute for Medical Research, Sir Mortimer B, Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
Retrovirology 3:93. 2006The HIV-1 Rev protein mediates nuclear export of unspliced and partially spliced viral RNA through interaction with the Rev response element (RRE) by means of an arginine rich motif that is similar to the one found in Tat...
- hnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expressionKathryn Woolaway
Department of Molecular and Medical Genetics, University of Toronto Toronto, Ontario, Canada
Retrovirology 4:28. 2007..We show that hnRNP E1/E2 are able to interact with the ESS3a element of the bipartite ESS in tat/rev exon 3 of HIV-1 and that modulation of hnRNP E1 expression alters HIV-1 structural protein synthesis...
- Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and RevSergei Nekhai
Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biolology, Howard University, NW Washington, DC 20059, USA
Future Microbiol 1:417-26. 2006..This article reviews the interplay between viral proteins Tat and Rev and their cellular cofactors in the transcriptional and post-transcriptional regulation of HIV-1 gene expression...
- An anti-apoptotic protein, Hax-1, inhibits the HIV-1 rev function by altering its sub-cellular localizationSuhasini Modem
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
J Cell Physiol 214:14-9. 2008The human immunodeficiency virus type 1 (HIV-1) Rev protein facilitates the nuclear export of viral mRNA containing the Rev response element (RRE)...
- Knockdown of cellular RNA helicase DDX3 by short hairpin RNAs suppresses HIV-1 viral replication without inducing apoptosisMusarat Ishaq
State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China
Mol Biotechnol 39:231-8. 2008..The DEAD-box RNA helicase DDX3 is an essential Rev co-factor in the CRM1-Rev-RRE complex that promotes the export of unspliced and single-spliced HIV-1 RNAs from the ..
- Role of cellular RNA processing factors in human immunodeficiency virus type 1 mRNA metabolism, replication, and infectivityJoseph A Jablonski
Basic Science Department, College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA
J Virol 83:981-92. 2009..This work demonstrates that cellular factors regulating mRNA processing have wide-ranging effects on human immunodeficiency virus type 1 replication and should be considered novel therapeutic targets...
- Cellular proteins and HIV-1 Rev functionModem Suhasini
Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA
Curr HIV Res 7:91-100. 2009..This process, mediated by the Rev protein, interfaces with cellular components involved in post-transcriptional gene regulation...
- Inhibition of HIV-1 gene expression by Sam68 Delta C: multiple targets but a common mechanism?Alan Cochrane
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
Retrovirology 6:22. 2009..Understanding how this Sam68 mutant discriminates among the multiple viral mRNAs promises to reveal unique properties of HIV-1 RNA metabolism...
- Sam68 functions in nuclear export and translation of HIV-1 RNAJohnny J He
Center for AIDS Research and Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
RNA Biol 6:384-6. 2009..is mainly controlled at the transcriptional level by HIV-1 Tat and at the post-transcriptional level by HIV-1 Rev. A number of host factors have been identified to be involved in these processes...
- Typhoon 8600 Variable Mode Imager WorkstationJeffrey Wilusz; Fiscal Year: 2002..abstract_text> ..
- Regulation of mRNA Decapping in Human CellsJeffrey Wilusz; Fiscal Year: 2004..In total, these studies should provide pivotal insights into this important area of post-transcriptional regulation of gene expression in mammalian cells. ..
- MECHANISMS OF HIV-1 ENTRY AND PATHOGENESIS IN ASTROCYTESJohnny He; Fiscal Year: 2008..They should also aid in the development of strategies for treating neurologic symptoms of AIDS patients. ..
- Virus-Mosquito mRNA StabilityJeffrey Wilusz; Fiscal Year: 2009..In summary, this information will provide fundamental insights into insect molecular biology and virus-host interactions that will provide the groundwork for novel avenues of arbovirus control. ..
- MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATIONAlan Engelman; Fiscal Year: 2001..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
- DELETION MUTAGENESIS WITHIN NONCODING SEGMENTS OF HIV1Mark Wainberg; Fiscal Year: 2001..6. To determine whether these attenuated SIV constructs may protect against subsequent challenge with virulent forms of SIV and SIV/HIV hybrids. ..
- LEDGF-Integrase Structural BiologyAlan Engelman; Fiscal Year: 2005..abstract_text> ..
- Tat Interaction with LRP and Its Role in AIDS DementiaJohnny He; Fiscal Year: 2006..In addition, these studies will also yield new clues for developing anti-HIV therapeutic strategies for treating HIV-associated neurological disorders occurring in the majority of AIDS patients. ..
- Use a novel Tat transgenic model to develop neuroAIDS therapeuticsJohnny He; Fiscal Year: 2007..The proposed study is to explore the possibility of using a mouse model to develop, evaluate, and validate therapeutic strategies specifically targeted at these diseases. [unreadable] [unreadable] [unreadable]..
- Drug Abuse and NeuroAIDS in ChinaJohnny He; Fiscal Year: 2008..The social and economic impact can not be overemphasized. The current study seeks to have a better understanding of the prevalence and nature of these diseases in the selected population. [unreadable] [unreadable] [unreadable]..
- BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATIONAlan Engelman; Fiscal Year: 2009..abstract_text> ..