RAP1GDS1

Summary

Gene Symbol: RAP1GDS1
Description: Rap1 GTPase-GDP dissociation stimulator 1
Alias: GDS1, SmgGDS, rap1 GTPase-GDP dissociation stimulator 1, RAP1, GTP-GDP dissociation stimulator 1, SMG GDS protein, SMG P21 stimulatory GDP/GTP exchange protein, exchange factor smgGDS
Species: human

Top Publications

  1. ncbi SmgGDS regulates cell proliferation, migration, and NF-kappaB transcriptional activity in non-small cell lung carcinoma
    Gaik Wei Tew
    Department of Pharmacology and Toxicology, Cardiovascular Center, Medical College of Wisconsin, Milwaukee, 53226, USA
    J Biol Chem 283:963-76. 2008
  2. ncbi Molecular cloning of the human cDNA for a stimulatory GDP/GTP exchange protein for c-Ki-ras p21 and smg p21
    A Kikuchi
    Department of Biochemistry, Kobe University School of Medicine, Japan
    Oncogene 7:289-93. 1992
  3. pmc Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
    Wei Wu
    Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China
    Biomed Res Int 2016:2789245. 2016
  4. pmc SmgGDS-558 regulates the cell cycle in pancreatic, non-small cell lung, and breast cancers
    Nathan J Schuld
    Department of Pharmacology and Toxicology Medical College of Wisconsin Milwaukee, WI USA
    Cell Cycle 13:941-52. 2014
  5. doi NUP98/11p15 translocations affect CD34+ cells in myeloid and T lymphoid leukemias
    Barbara Crescenzi
    Laboratory of Molecular Medicine, CREO, University of Perugia and A O Perugia, 06132 Perugia, Italy
    Leuk Res 39:769-72. 2015
  6. doi Statins up-regulate SmgGDS through β1-integrin/Akt1 pathway in endothelial cells
    Tatsuro Minami
    Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, Izunokuni, Japan
    Cardiovasc Res 109:151-61. 2016
  7. pmc The Tumor-suppressive Small GTPase DiRas1 Binds the Noncanonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases
    Carmen Bergom
    From the Cancer Center, The Departments of Radiation Oncology
    J Biol Chem 291:6534-45. 2016
  8. doi SmgGDS as a Crucial Mediator of the Inhibitory Effects of Statins on Cardiac Hypertrophy and Fibrosis: Novel Mechanism of the Pleiotropic Effects of Statins
    Shun Kudo
    From the Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
    Hypertension 67:878-89. 2016
  9. pmc microRNA regulatory circuits in a mouse model of inherited retinal degeneration
    Arpad Palfi
    Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland
    Sci Rep 6:31431. 2016
  10. pmc Vimar Is a Novel Regulator of Mitochondrial Fission through Miro
    Lianggong Ding
    State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing, China
    PLoS Genet 12:e1006359. 2016

Research Grants

  1. CAROL LUCILLE WILLIAMS; Fiscal Year: 2014
  2. REGULATION OF RHO AND RAC BY MUSCARINIC RECEPTORS
    Carol Williams; Fiscal Year: 2003
  3. Small GTPase Polybasic Regions: Function and Regulation
    Carol Williams; Fiscal Year: 2008

Scientific Experts

  • CAROL LUCILLE WILLIAMS
  • S P Romana
  • Ioannis Panagopoulos
  • Ellen L Lorimer
  • Andrew D Hauser
  • Nathan J Schuld
  • Shun Kudo
  • Tatsuro Minami
  • C Bergom
  • Carmen Bergom
  • Jessica M Wilson
  • Hiroaki Shimokawa
  • Shin ichi Tanaka
  • Elizabeth Ntantie
  • R Li
  • Tracy J Berg
  • Cathy Cole Lanning
  • Dae Won Kim
  • Arpad Palfi
  • Kimio Satoh
  • Jeremy W Prokop
  • Lianggong Ding
  • Wei Wu
  • Satoshi Miyata
  • Masamichi Nogi
  • Fotis Tsetsos
  • Yoshitaka Ogita
  • Barbara Crescenzi
  • Alexander C MacKinnon
  • Patrick Gonyo
  • Yu Fan Hsieh
  • Aarthi Ravichandran
  • Adam J Gastonguay
  • A C MacKinnon
  • E L Lorimer
  • Brant Hamel
  • A Hauser
  • Elaine A McSherry
  • Cristina Mecucci
  • Lidiya Orlichenko
  • Margret Shirinian
  • C Schönherr
  • Holger Rehmann
  • Tatijana Zemunik
  • Rongshan Li
  • H Zhi
  • María T Branham
  • Gaik Wei Tew
  • Rebecca Thill
  • Laura J C M van Zutven
  • Eun Young Shin
  • Wenyue Sun
  • Shuliang Shi
  • Sanjay Tiwari
  • Yuri N Kobzev
  • Rebecca Ruiz-Velasco
  • Junichi Omura
  • Xunming Ji
  • Kathleen Noon
  • Jian lin Lou
  • Iordanis Karagiannidis
  • John Alexander
  • Ye Lei
  • Dimitrios Mantzaris
  • Yun Xiao
  • Sophia Millington-Ward
  • Amy Rymaszewski
  • Jia Ren Liu
  • Shohei Ikeda
  • Petros Drineas
  • Catherine M Greene
  • Elod Kortvely
  • Taijyu Satoh
  • Li jin Zhu
  • Hai Ling Xia
  • Sung Min Hwang
  • Shanmukha S Padmanabhuni
  • Marianthi Georgitsi
  • Kota Suzuki
  • Naomi Chadderton
  • Margaritis Tsifintaris
  • Idoya Lahortiga
  • Peristera Paschou
  • Stefanie M Hauck
  • Jong Chan Hong
  • Irene Aguilera-Barrantes
  • Paul F Kenna
  • Sebastian Vencken
  • Jeong Dong Bahk
  • Kim M T de Bruyn

Detail Information

Publications55

  1. ncbi SmgGDS regulates cell proliferation, migration, and NF-kappaB transcriptional activity in non-small cell lung carcinoma
    Gaik Wei Tew
    Department of Pharmacology and Toxicology, Cardiovascular Center, Medical College of Wisconsin, Milwaukee, 53226, USA
    J Biol Chem 283:963-76. 2008
    ..b>SmgGDS is an unusual guanine nucleotide exchange factor that activates many of these small GTPases, and thus may promote ..
  2. ncbi Molecular cloning of the human cDNA for a stimulatory GDP/GTP exchange protein for c-Ki-ras p21 and smg p21
    A Kikuchi
    Department of Biochemistry, Kobe University School of Medicine, Japan
    Oncogene 7:289-93. 1992
    ..The isolated cDNA is expressed in Escherichia coli, and the encoded protein shows the physical and functional properties similar to those of bovine smg GDS...
  3. pmc Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
    Wei Wu
    Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China
    Biomed Res Int 2016:2789245. 2016
    ..We also found increased mRNA level of RAP1GDS1, a critical factor of metabolism that has been identified as a fusion partner in various hematopoietic ..
  4. pmc SmgGDS-558 regulates the cell cycle in pancreatic, non-small cell lung, and breast cancers
    Nathan J Schuld
    Department of Pharmacology and Toxicology Medical College of Wisconsin Milwaukee, WI USA
    Cell Cycle 13:941-52. 2014
    ..Splice variants of SmgGDS, named SmgGDS-607 and SmgGDS-558, promote the prenylation and membrane trafficking of multiple Ras and Rho family ..
  5. doi NUP98/11p15 translocations affect CD34+ cells in myeloid and T lymphoid leukemias
    Barbara Crescenzi
    Laboratory of Molecular Medicine, CREO, University of Perugia and A O Perugia, 06132 Perugia, Italy
    Leuk Res 39:769-72. 2015
    ..e. NSD1, DDX10, RAP1GDS1, and LNP1, NUP98 translocations always affected a CD34+/CD133+ hematopoietic precursor...
  6. doi Statins up-regulate SmgGDS through β1-integrin/Akt1 pathway in endothelial cells
    Tatsuro Minami
    Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, Izunokuni, Japan
    Cardiovasc Res 109:151-61. 2016
    ..effects of statins are partly mediated through up-regulation of small GTP-binding protein dissociation stimulator (SmgGDS) with a resultant Rac1 degradation and reduced oxidative stress...
  7. pmc The Tumor-suppressive Small GTPase DiRas1 Binds the Noncanonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases
    Carmen Bergom
    From the Cancer Center, The Departments of Radiation Oncology
    J Biol Chem 291:6534-45. 2016
    ..In this study, we demonstrate that DiRas1 binds to SmgGDS, a protein that promotes the activation of several oncogenic GTPases...
  8. doi SmgGDS as a Crucial Mediator of the Inhibitory Effects of Statins on Cardiac Hypertrophy and Fibrosis: Novel Mechanism of the Pleiotropic Effects of Statins
    Shun Kudo
    From the Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
    Hypertension 67:878-89. 2016
    ..that cardioprotective effects of statins are mediated by small GTP-binding protein GDP dissociation stimulator (SmgGDS)...
  9. pmc microRNA regulatory circuits in a mouse model of inherited retinal degeneration
    Arpad Palfi
    Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland
    Sci Rep 6:31431. 2016
    ..Furthermore, levels of ~25% of the retinal Rac1 interactors were determined by LC-MS/MS; expression of Rap1gds1 and Cav1 was elevated. Our data suggest significant utilisation of miRNA-based regulation in retina...
  10. pmc Vimar Is a Novel Regulator of Mitochondrial Fission through Miro
    Lianggong Ding
    State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing, China
    PLoS Genet 12:e1006359. 2016
    ..Furthermore, we found that the mammalian homolog of Vimar, RAP1GDS1, played a similar role in regulating mitochondrial morphology, suggesting a functional conservation of this GEF ..
  11. ncbi SmgGDS splice variants: Potential novel targets for breast cancer therapeutics
    C Bergom
    Medical College of Wisconsin, Milwaukee, WI Plus Diagnostics, Union, NJ
    J Clin Oncol 29:10567. 2011
    ..We recently reported that the activity of these GTPases is regulated by two splice variants of SmgGDS, named SmgGDS-558 and SmgGDS-607...
  12. ncbi The (4;11)(q21;p15) translocation fuses the NUP98 and RAP1GDS1 genes and is recurrent in T-cell acute lymphocytic leukemia
    D J Hussey
    Department of Haematology Oncology, University of Adelaide, The Queen Elizabeth Hospital, Woodville, Australia
    Blood 94:2072-9. 1999
    ..The fusion partner of NUP98 was identified as the RAP1GDS1 gene using 3' RACE...
  13. doi The C3H-type zinc finger protein GDS1/C3H42 is a nuclear-speckle-localized protein that is essential for normal growth and development in Arabidopsis
    Dae Won Kim
    Division of Applied Life Science BK21plus, PMBBRC, Graduate School of Gyeongsang National University, Jinju 660 701, Republic of Korea
    Plant Sci 250:141-53. 2016
    ..However, compared to mammalian, only a few plant Znfs have been functionally characterized. Here, T-DNA inserted gds1 (growth, development and splicing 1) mutant, displayed abnormal growth throughout the lifecycle owing to the ..
  14. pmc Meta-Analysis of Tourette Syndrome and Attention Deficit Hyperactivity Disorder Provides Support for a Shared Genetic Basis
    Fotis Tsetsos
    Department of Molecular Biology and Genetics, Democritus University of Thrace Alexandroupolis, Greece
    Front Neurosci 10:340. 2016
    ..We identify 19 significant SNPs, with the top four implicated genes being TBC1D7, GUCY1A3, RAP1GDS1, and CHST11. TBCD17 harbors the top scoring SNP, rs1866863 (p:3...
  15. pmc Di-Ras2 Protein Forms a Complex with SmgGDS Protein in Brain Cytosol in Order to Be in a Low Affinity State for Guanine Nucleotides
    Yoshitaka Ogita
    From the Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113 0033, Japan
    J Biol Chem 290:20245-56. 2015
    ..In the present study we found that endogenous Di-Ras2 co-purifies with SmgGDS from rat brain cytosol...
  16. pmc Differences in the Phosphorylation-Dependent Regulation of Prenylation of Rap1A and Rap1B
    Jessica M Wilson
    Department of Pharmacology and Toxicology, Cancer Center and Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Mol Biol 428:4929-4945. 2016
    ..versus Rap1B, due in part to their different phosphorylation-dependent interactions with the chaperone protein SmgGDS-607...
  17. ncbi SmgGDS splice variants: Potential novel targets for breast cancer therapeutics
    C Bergom
    Medical College of Wisconsin, Milwaukee, WI Plus Diagnostics, Union, NJ
    J Clin Oncol 29:225. 2011
    ..We recently reported that the activity of these GTPases is regulated by two splice variants of SmgGDS, named SmgGDS-558 and SmgGDS-607...
  18. pmc The chaperone protein SmgGDS interacts with small GTPases entering the prenylation pathway by recognizing the last amino acid in the CAAX motif
    Nathan J Schuld
    From the Departments of Pharmacology and Toxicology
    J Biol Chem 289:6862-76. 2014
    ..We previously reported that SmgGDS splice variants promote prenylation and trafficking of GTPases containing a C-terminal polybasic region and ..
  19. pmc The SmgGDS splice variant SmgGDS-558 is a key promoter of tumor growth and RhoA signaling in breast cancer
    Andrew D Hauser
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226
    Mol Cancer Res 12:130-42. 2014
    Breast cancer malignancy is promoted by the small GTPases RhoA and RhoC. SmgGDS is a guanine nucleotide exchange factor that activates RhoA and RhoC in vitro...
  20. pmc Breast cancer cell migration is regulated through junctional adhesion molecule-A-mediated activation of Rap1 GTPase
    Elaine A McSherry
    Department of Surgery, Royal College of Surgeons in Ireland, RCSI Education and Research Centre, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland
    Breast Cancer Res 13:R31. 2011
    ..As cell migration is an early requirement for tumor metastasis, we sought to identify the JAM-A signalling events regulating migration in breast cancer cells...
  21. pmc SmgGDS antagonizes BPGAP1-induced Ras/ERK activation and neuritogenesis in PC12 cell differentiation
    Aarthi Ravichandran
    Cell Signaling and Developmental Biology Laboratory, Department of Biological Sciences, National University of Singapore, Singapore 117543, Republic of Singapore
    Mol Biol Cell 24:145-56. 2013
    ..mutants of Mek2 (Mek2-K101A) and K-Ras (K-Ras-S17N) and also by the small G-protein GDP dissociation stimulator (SmgGDS)...
  22. pmc Statins exert the pleiotropic effects through small GTP-binding protein dissociation stimulator upregulation with a resultant Rac1 degradation
    Shin ichi Tanaka
    Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan
    Arterioscler Thromb Vasc Biol 33:1591-600. 2013
    ..In this study, we tested our hypothesis that small GTP-binding protein GDP dissociation stimulator (SmgGDS) plays a crucial role in the molecular mechanisms of the Rac1 signaling pathway inhibition by statins in ..
  23. pmc An adenosine-mediated signaling pathway suppresses prenylation of the GTPase Rap1B and promotes cell scattering
    Elizabeth Ntantie
    1Department of Pharmacology and Toxicology, Cancer Center, and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Sci Signal 6:ra39. 2013
    ..prenylation of Rap1B through phosphorylation of Rap1B, which decreased its interaction with the chaperone protein SmgGDS (small GTPase guanosine diphosphate dissociation stimulator)...
  24. ncbi Identification of differentially expressed genes in human lung squamous cell carcinoma using suppression subtractive hybridization
    Wenyue Sun
    Department of Etiology and Carcinogenesis, Cancer Institute Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, P O Box 2258, Beijing 100021, China
    Cancer Lett 212:83-93. 2004
    ..Among them, the IQGAP1, RAP1GDS1, PAICS, MLF1, and MARK1 genes showed a consistent expression pattern with that of the SSH analysis...
  25. pmc Transglutaminase 2 contributes to apoptosis induction in Jurkat T cells by modulating Ca2+ homeostasis via cross-linking RAP1GDS1
    Yu Fan Hsieh
    Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan
    PLoS ONE 8:e81516. 2013
    ..Transglutaminase 2 (TG2) is a protein cross-linking enzyme known to be associated with the in vivo apoptosis program of T cells. However, its role in the T cell apoptosis program was not investigated yet...
  26. ncbi Identification of NUP98 abnormalities in acute leukemia: JARID1A (12p13) as a new partner gene
    Laura J C M van Zutven
    Department of Genetics, Centre for Biomedical Genetics, Erasmus MC, Rotterdam, The Netherlands
    Genes Chromosomes Cancer 45:437-46. 2006
    ..both showing 11p abnormalities in the diagnostic karyotype: a t(4;11)(q1?3;p15) with expression of the NUP98-RAP1GDS1 fusion product detected in a 60-year-old woman with AML-M0, and an add(11)(p15) with a der(21)t(11;21)(p15;p13) ..
  27. ncbi Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangements
    Yuri N Kobzev
    Section of Hematology Oncology, Department of Medicine, Biological Sciences Division, University of Chicago, 5841 S Maryland Avenue, Chicago, IL 60637, USA
    Genes Chromosomes Cancer 41:339-52. 2004
    ..FISH probes, in 6 cases, we identified the partner genes, which were PRRX1 (PMX1; in 2 cases), HOXD13, RAP1GDS1, HOXC13, and TOP1...
  28. ncbi The Rac1 C-terminal polybasic region regulates the nuclear localization and protein degradation of Rac1
    Cathy Cole Lanning
    Molecular Pharmacology Laboratory, Guthrie Research Institute, Sayre, Pennsylvania 18840, USA
    J Biol Chem 279:44197-210. 2004
    ..Rac1(pbrRhoA) does not greatly differ from Rac1 in its ability to stimulate membrane ruffling or to interact with SmgGDS and IQGAP1-calmodulin complexes...
  29. ncbi Novel mechanism of the co-regulation of nuclear transport of SmgGDS and Rac1
    Cathy Cole Lanning
    Molecular Pharmacology Laboratory, Guthrie Research Institute, Sayre, Pennsylvania 18840, USA
    J Biol Chem 278:12495-506. 2003
    The armadillo protein SmgGDS promotes guanine nucleotide exchange by small GTPases containing a C-terminal polybasic region (PBR), such as Rac1 and RhoA...
  30. pmc Genome-wide association study of biochemical traits in Korcula Island, Croatia
    Tatijana Zemunik
    University of Split School of Medicine, Soltanska 2, 21000 Split, Croatia
    Croat Med J 50:23-33. 2009
    ....
  31. pmc SmgGDS is a guanine nucleotide exchange factor that specifically activates RhoA and RhoC
    Brant Hamel
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7365, USA
    J Biol Chem 286:12141-8. 2011
    b>SmgGDS is an atypical guanine nucleotide exchange factor (GEF) that promotes both cell proliferation and migration and is up-regulated in several types of cancer...
  32. pmc Splice variants of SmgGDS control small GTPase prenylation and membrane localization
    Tracy J Berg
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 285:35255-66. 2010
    ..entrance and passage of these small GTPases through the prenylation pathway is regulated by two splice variants of SmgGDS, a protein that has been reported to promote GDP/GTP exchange by PBR-possessing GTPases and to be up-regulated in ..
  33. ncbi The polybasic region of Ras and Rho family small GTPases: a regulator of protein interactions and membrane association and a site of nuclear localization signal sequences
    Carol L Williams
    Molecular Pharmacology Laboratory, Guthrie Research Institute, One Guthrie Square, Sayre, PA 18840, USA
    Cell Signal 15:1071-80. 2003
    ..The PBR promotes the interactions of small GTPases with SmgGDS, which is a nucleocytoplasmic shuttling protein that stimulates guanine nucleotide exchange by small GTPases...
  34. ncbi Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins
    T Yamamoto
    Department of Biochemistry, Kobe University School of Medicine, Japan
    J Biol Chem 265:16626-34. 1990
    ..smg p21 GDS1 and -2 also stimulated the binding of [35S]GTP gamma S to the GDP-bound form of smg p21s but not that to the ..
  35. ncbi Chromosomal assignment of the human smg GDP dissociation stimulator gene to human chromosome 4q21-q25
    O Riess
    Abteilung für Molekulare Humangenetik, Ruhr Universitat, Bochum, Germany
    Hum Genet 92:629-30. 1993
    ..This chromosomal localization of smg GDP to 4q21-25 overlaps with a region of allele loss in primary hepatocellular carcinoma (4q13-q26)...
  36. ncbi Nucleotide sequence of the GDS1 gene of Saccharomyces cerevisiae
    A Konopinska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Yeast 11:1513-8. 1995
    We have cloned and sequenced the GDS1 gene located on the right arm of chromosome XV of Saccharomyces cerevisiae. The gene codes for a 522 amino acid serine-rich protein with no obvious homology to proteins in the database...
  37. ncbi SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase
    K Shimizu
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
    J Biol Chem 271:27013-7. 1996
    ..These results suggest that SMAP serves as an adaptor for both Smg GDS and kinesin II or its related protein and links them with both the Smg GDS-regulated small G protein and Src tyrosine kinase signalings...
  38. ncbi YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance
    X Pan
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    J Cell Sci 111:2137-47. 1998
    ..By analogy to the function of beta-catenin in cell-cell adhesion, alpha-karyopherin in nuclear transport, and smgGDS in the control of ras-like GTPases, Yeb3p may provide a link between vacuoles and the actin cytoskeleton during ..
  39. ncbi Identification and characterization in Xenopus of XsmgGDS, a RalB binding protein
    N Iouzalen
    Institut Jacques Monod CNRS, Universite Paris VII, France
    Biochem Biophys Res Commun 250:359-63. 1998
    ..We have identified a full-length cDNA encoding a protein homologous to mammalian smgGDS. The Xenopus gene shows 84.6% identity with the mammalian counterpart and contains one additional armadillo repeat...
  40. pmc Identification of darlin, a Dictyostelium protein with Armadillo-like repeats that binds to small GTPases and is important for the proper aggregation of developing cells
    K K Vithalani
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 9:3095-106. 1998
    ..revealed that it contained multiple armadillo-like repeats most closely related to the mammalian GTP exchange factor smgGDS. We named this protein darlin (Dictyostelium armadillo-like protein)...
  41. ncbi SmgGDS displays differential binding and exchange activity towards different Ras isoforms
    Haris G Vikis
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, MI 48109 0606, USA
    Oncogene 21:2425-32. 2002
    ..for novel guanine nucleotide exchange factors of HRas and isolated small G-protein dissociation stimulator (smgGDS), a guanine nucleotide exchange factor known to act on numerous Ras and Rho family GTPases...
  42. ncbi Fusion of the NUP98 gene and the homeobox gene HOXC13 in acute myeloid leukemia with t(11;12)(p15;q13)
    Ioannis Panagopoulos
    Department of Clinical Genetics, Lund University Hospital, Sweden
    Genes Chromosomes Cancer 36:107-12. 2003
    ..gene at 11p15 is known to be fused to DDX10, HOXA9, HOXA11, HOXA13, HOXD11, HOXD13, LEDGF, NSD1, NSD3, PMX1, RAP1GDS1, and TOP1 in various hematologic malignancies...
  43. ncbi The small GTPase Rap1 is activated by turbulence and is involved in integrin [alpha]IIb[beta]3-mediated cell adhesion in human megakaryocytes
    Kim M T de Bruyn
    Department of Physiological Chemistry, University Medical Center, Utrecht, The Netherlands
    J Biol Chem 278:22412-7. 2003
    ..From these results we conclude that in megakaryocytes turbulence induces Rap1 activation that controls alphaIIbbeta3-mediated cell adhesion...
  44. ncbi NUP98 is fused to adducin 3 in a patient with T-cell acute lymphoblastic leukemia and myeloid markers, with a new translocation t(10;11)(q25;p15)
    Idoya Lahortiga
    Department of Genetics, University of Navarra, 31008 Pamplona, Spain
    Cancer Res 63:3079-83. 2003
    ..Twelve of them have been identified in patients with myeloid neoplasias and only 1, RAP1GDS1 (4q21), is fused with NUP98 in five patients with T-cell acute lymphoblastic leukemia (T-ALL)...
  45. pmc The 19-amino acid insertion in the tumor-associated splice isoform Rac1b confers specific binding to p120 catenin
    Lidiya Orlichenko
    Department of Cancer Biology, Mayo Clinic, Jacksonville, Florida 32224, USA
    J Biol Chem 285:19153-61. 2010
    ..Rac1b does not bind to many common regulators of Rho family GTPases but that it does display enhanced binding to SmgGDS, RACK1, and p120 catenin (p120(ctn)), proteins involved in cell-cell adhesion, motility, and transcriptional ..
  46. ncbi Among circulating hematopoietic cells, B-CLL uniquely expresses functional EPAC1, but EPAC1-mediated Rap1 activation does not account for PDE4 inhibitor-induced apoptosis
    Sanjay Tiwari
    Department of Medicine, Section of Hematology Oncology, Boston Medical Center, MA 02118, USA
    Blood 103:2661-7. 2004
    ..002). Our results demonstrate that B-CLL cells uniquely activate Rap1 in response to PDE4 inhibitors and suggest that physiologic stimuli that activate EPAC may transmit an antiapoptotic signal...
  47. ncbi Rap1 mutants with increased affinity for the guanine-nucleotide exchange factor C3G
    Shuliang Shi
    Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Oncogene 23:8711-9. 2004
    ..On the other hand, Rap1 activation mediated by two other GEFs, Epac and smgGDS, was not inhibited by Rap1-AGE...
  48. ncbi NUP98 rearrangements in hematopoietic malignancies: a study of the Groupe Francophone de Cytogénétique Hématologique
    S P Romana
    Service de Cytogénétique, Centre Hospitalier Universitaire CHU Necker Enfants Malades, Paris, France
    Leukemia 20:696-706. 2006
    ..were detected, respectively, in myeloid disorders and in T-cell acute lymphoblastic leukemia (T-ALL), whereas the RAP1GDS1 gene was fused to NUP98 in T-ALL. Three new chromosomal breakpoints: 3q22...
  49. ncbi betaPak-interacting exchange factor-mediated Rac1 activation requires smgGDS guanine nucleotide exchange factor in basic fibroblast growth factor-induced neurite outgrowth
    Eun Young Shin
    Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361 763, Korea
    J Biol Chem 281:35954-64. 2006
    ..We show here that betaPIX binds to Rac1 via the GBD and also activates the GTPase via an associated GEF, smgGDS, in a phosphorylation-dependent manner...
  50. ncbi Fusion of NUP98 and the SET binding protein 1 (SETBP1) gene in a paediatric acute T cell lymphoblastic leukaemia with t(11;18)(p15;q12)
    Ioannis Panagopoulos
    Department of Clinical Genetics, University Hospital, Lund, Sweden
    Br J Haematol 136:294-6. 2007
    ..previously been reported in T cell acute lymphoblastic leukaemia (T-ALL): NUP98/ADD3, NUP98/CCDC28A, and NUP98/RAP1GDS1. We report a T-ALL with t(11;18)(p15;q12) resulting in a novel NUP98 fusion...
  51. doi Identification and characterization of the unique guanine nucleotide exchange factor, SmgGDS, in vascular smooth muscle cells
    Rebecca Thill
    Department of Pharmacology and Toxicology, Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Cell Biochem 104:1760-70. 2008
    The guanine nucleotide exchange factor (GEF), SmgGDS, promotes nucleotide exchange by several GTPases in both the Ras and Rho families, especially by RhoA...
  52. doi SmgGDS is up-regulated in prostate carcinoma and promotes tumour phenotypes in prostate cancer cells
    H Zhi
    Department of Pathology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Pathol 217:389-97. 2009
    b>SmgGDS is a guanine nucleotide exchange factor with the unique ability to activate multiple small GTPases, implicating it in cancer development and progression...
  53. pmc Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis
    María T Branham
    Laboratorio de Biologia Celular y Molecular, Instituto de Histología y Embriología Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Medicas, CC 56, Universidad Nacional de Cuyo, 5500 Mendoza, Argentina
    J Biol Chem 284:24825-39. 2009
    ..We propose that Epac sits at a critical point during the exocytotic cascade after which the pathway splits into two limbs, one that assembles the fusion machinery into place and another that elicits intracellular calcium release...
  54. doi Anaplastic lymphoma kinase activates the small GTPase Rap1 via the Rap1-specific GEF C3G in both neuroblastoma and PC12 cells
    C Schönherr
    Department of Molecular Biology, Umea University, Umea, Sweden
    Oncogene 29:2817-30. 2010
    ..These results suggest that ALK activation of Rap1 may contribute to cell proliferation and oncogenesis of neuroblastoma driven by gain-of-function mutant ALK receptors...
  55. pmc The Rap1 guanine nucleotide exchange factor C3G is required for preservation of larval muscle integrity in Drosophila melanogaster
    Margret Shirinian
    Department of Molecular Biology, Umea University, Umea, Sweden
    PLoS ONE 5:e9403. 2010
    ..Taken together, we conclude that Drosophila C3G is a Rap1-specific GEF with important functions in maintaining muscle integrity during larval stages...

Research Grants3

  1. CAROL LUCILLE WILLIAMS; Fiscal Year: 2014
    ..Our research indicates that the unique guanine nucleotide exchange factor known as SmgGDS is a major regulator of small GTPase activity in NSCLC...
  2. REGULATION OF RHO AND RAC BY MUSCARINIC RECEPTORS
    Carol Williams; Fiscal Year: 2003
    ..alters the association altered by activating M3 mAChR or protein kinase C (PKC), or by microinjecting cDNA or SmgGDS, which is a guanine nucleotide exchange factor (GEF) for RhoA...
  3. Small GTPase Polybasic Regions: Function and Regulation
    Carol Williams; Fiscal Year: 2008
    ..Many of these small GTPases are activated by the guanine nucleotide exchange factor SmgGDS. The ability of SmgGDS to activate multiple small GTPases belonging to different families is very unique, ..