RAD52

Summary

Gene Symbol: RAD52
Description: RAD52 homolog, DNA repair protein
Alias: DNA repair protein RAD52 homolog, recombination protein RAD52, rhabdomyosarcoma antigen MU-RMS-40.23
Species: human

Top Publications

  1. pmc A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52
    A A Firmenich
    Department of Biochemistry, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, California 94305
    Mol Cell Biol 15:1620-31. 1995
  2. ncbi Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM and c-Abl
    G Chen
    Department of Molecular Medicine Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    J Biol Chem 274:12748-52. 1999
  3. ncbi Regulation of ionizing radiation-induced Rad52 nuclear foci formation by c-Abl-mediated phosphorylation
    Hiroyuki Kitao
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 277:48944-8. 2002
  4. pmc Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence
    Mahrukh Azam
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Nucleic Acids Res 34:506-16. 2006
  5. ncbi Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair
    Sibgat A Choudhury
    Department of Oncology, Faculty of Medicine, McGill University, Montreal General Hospital, 1650 Avenue Cedar, Montreal, Quebec, Canada
    Mol Cell Biochem 300:215-26. 2007
  6. ncbi Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination
    D F Muris
    Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands
    Mutat Res 315:295-305. 1994
  7. ncbi Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells
    M S Park
    Life Sciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    J Biol Chem 271:18996-9000. 1996
  8. ncbi The human Rad52 protein exists as a heptameric ring
    A Z Stasiak
    Laboratory of Ultrastructural Analysis, University of Lausanne, Lausanne, CH 1015, Switzerland
    Curr Biol 10:337-40. 2000
  9. pmc Precise binding of single-stranded DNA termini by human RAD52 protein
    C A Parsons
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 19:4175-81. 2000
  10. ncbi Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA
    G Mer
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 103:449-56. 2000

Research Grants

  1. ROLES OF HUMAN RECOMBINATION GENES IN GENOME INSTABILITY
    David Chen; Fiscal Year: 2001
  2. Patrick Sung; Fiscal Year: 2016
  3. Simon N Powell; Fiscal Year: 2016
  4. Recombination and fork progression in bacteriophage T4
    Stephen W White; Fiscal Year: 2013
  5. Role of RPA Hyperphosphorylation in DNA Damage Responses
    MOISES ALEJANDRO SERRANO; Fiscal Year: 2012
  6. Development of RAD52 Inhibitors to Induce Lethality of BRCA2-Deficient Cells
    Alexander V Mazin; Fiscal Year: 2013
  7. RODNEY J ROTHSTEIN; Fiscal Year: 2014
  8. WILLIAM GAINES; Fiscal Year: 2014
  9. Philip A Hieter; Fiscal Year: 2016
  10. GENETIC AND PHYSICAL ANALYSIS OF RECOMBINATION REPAIR
    Jac Nickoloff; Fiscal Year: 2002

Detail Information

Publications193 found, 100 shown here

  1. pmc A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52
    A A Firmenich
    Department of Biochemistry, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, California 94305
    Mol Cell Biol 15:1620-31. 1995
    ..Characterization of rfa1-44 revealed that it is, like members of the RAD52 epistasis group, sensitive to X rays, high doses of UV, and HO-induced DSBs...
  2. ncbi Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM and c-Abl
    G Chen
    Department of Molecular Medicine Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    J Biol Chem 274:12748-52. 1999
    ..assays using purified components, phosphorylation of Rad51 by c-Abl enhances complex formation between Rad51 and Rad52, which cooperates with Rad51 in recombination and repair...
  3. ncbi Regulation of ionizing radiation-induced Rad52 nuclear foci formation by c-Abl-mediated phosphorylation
    Hiroyuki Kitao
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 277:48944-8. 2002
    The RAD52 epistasis group of proteins, including Rad51, Rad52, and Rad54, plays an important role in the homologous recombination repair of double strand breaks...
  4. pmc Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence
    Mahrukh Azam
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Nucleic Acids Res 34:506-16. 2006
    ..sgs1 and rad52 mutations are epistatic during senescence, indicating that Sgs1p participates in a RAD52-dependent recombinational ..
  5. ncbi Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair
    Sibgat A Choudhury
    Department of Oncology, Faculty of Medicine, McGill University, Montreal General Hospital, 1650 Avenue Cedar, Montreal, Quebec, Canada
    Mol Cell Biochem 300:215-26. 2007
    ..the TRM2 gene) displayed low sensitivity to methyl methane sulfonate (MMS) and suppressed the MMS sensitivity of rad52 mutants in trm2(Delta232-1920nt)rad52 double mutants...
  6. ncbi Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination
    D F Muris
    Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands
    Mutat Res 315:295-305. 1994
    The RAD52 gene of Saccharomyces cerevisiae is required for recombinational repair of double-strand breaks...
  7. ncbi Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells
    M S Park
    Life Sciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    J Biol Chem 271:18996-9000. 1996
    The yeast RAD52 protein is essential for DNA double-strand break repair, and meiotic and mitotic recombination...
  8. ncbi The human Rad52 protein exists as a heptameric ring
    A Z Stasiak
    Laboratory of Ultrastructural Analysis, University of Lausanne, Lausanne, CH 1015, Switzerland
    Curr Biol 10:337-40. 2000
    The RAD52 epistasis group was identified in yeast as a group of genes required to repair DNA damaged by ionizing radiation [1]...
  9. pmc Precise binding of single-stranded DNA termini by human RAD52 protein
    C A Parsons
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 19:4175-81. 2000
    The human RAD52 protein, which exhibits a heptameric ring structure, has been shown to bind resected double strand breaks (DSBs), consistent with an early role in meiotic recombination and DSB repair...
  10. ncbi Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA
    G Mer
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 103:449-56. 2000
    ..a specific surface that interacts in a similar manner with the DNA repair enzyme UNG2 and repair factors XPA and RAD52, each of which functions in a different repair pathway...
  11. ncbi Human RAD52 protein has extreme thermal stability
    W Ranatunga
    Department of Chemistry, The University of Toledo, Toledo, Ohio 43606 3390, USA
    Biochemistry 40:8557-62. 2001
    The human RAD52 protein plays an important role in the earliest stages of chromosomal double-strand break repair via the homologous recombination pathway. Individual subunits of RAD52 associate into seven-membered rings...
  12. pmc Visualization of recombination intermediates produced by RAD52-mediated single-strand annealing
    E Van Dyck
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO Rep 2:905-9. 2001
    ..In yeast, DSB repair requires RAD52, a protein that plays a critical role in homologous recombination...
  13. pmc Overexpression of human RAD51 and RAD52 reduces double-strand break-induced homologous recombination in mammalian cells
    P M Kim
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Nucleic Acids Res 29:4352-60. 2001
    ..RAD51 functions with RAD52 and other proteins to effect strand exchange during HR, forming heteroduplex DNA (hDNA) that is resolved by ..
  14. pmc Differential effects of Rad52p overexpression on gene targeting and extrachromosomal homologous recombination in a human cell line
    Rafael J Yáñez
    Gene Targeting Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK
    Nucleic Acids Res 30:740-8. 2002
    Overexpression of the RAD52 epistasis group of gene products is a convenient way to investigate their in vivo roles in homologous recombination (HR) and DNA repair...
  15. ncbi Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation
    Tomohiko Sugiyama
    Section of Microbiology and Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 277:31663-72. 2002
    ..contrast, RPA inhibits Rad51 nucleoprotein complex formation by prior binding to single-stranded DNA (ssDNA), but Rad52 protein alleviates this inhibition...
  16. ncbi Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form
    Wataru Kagawa
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama, Japan
    Mol Cell 10:359-71. 2002
    The human Rad52 protein forms a heptameric ring that catalyzes homologous pairing...
  17. pmc Structure of the single-strand annealing domain of human RAD52 protein
    Martin R Singleton
    Cancer Research U K, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Proc Natl Acad Sci U S A 99:13492-7. 2002
    In eukaryotic cells, RAD52 protein plays a central role in genetic recombination and DNA repair by (i) promoting the annealing of complementary single-stranded DNA and (ii) stimulation of the RAD51 recombinase...
  18. ncbi Human Rad52 facilitates a three-stranded pairing that follows no strand exchange: a novel pairing function of the protein
    Vasundhara M Navadgi
    Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India
    Biochemistry 42:15237-51. 2003
    Human Rad52 protein, by analogy with the genetics of yeast Rad52, is believed to mediate a pathway of homologous recombination even independent of Rad51...
  19. pmc Strand exchange activity of human recombination protein Rad52
    Jaspal K Kumar
    Department of Biological Sciences, University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA
    Proc Natl Acad Sci U S A 101:9562-7. 2004
    ..In eukaryotes, double-strand-break repair by homologous recombination requires the Rad52 group of proteins...
  20. ncbi Identification of residues important for DNA binding in the full-length human Rad52 protein
    Janice A Lloyd
    Department of Biochemistry and Molecular Pharmacology, Aaron Lazare Research Building, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605 2324, USA
    J Mol Biol 345:239-49. 2005
    Human Rad52 (HsRad52) is a DNA-binding protein (418 residues) that promotes the catalysis of DNA double strand break repair by the Rad51 recombinase...
  21. ncbi Effect of DNA sequence and nucleotide cofactors on hRad51 binding to ssDNA: role of hRad52 in recruitment
    Vasundhara M Navadgi
    Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India
    Biochem Biophys Res Commun 334:696-701. 2005
    ..Moreover, all the cooperative effects of hRad52 on hRad51 binding are highly specific to the latter's binding to ssDNA and not to dsDNA. These results help us to model important mechanistic steps of hRad51 presynapsis on ssDNA templates...
  22. ncbi DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52
    Vasundhara M Navadgi
    Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India
    FEBS J 273:199-207. 2006
    Purified human Rad51 and Rad52 proteins exhibit multiple oligomeric states, in vitro. Single-stranded DNA (ssDNA) renders high molecular weight aggregates of both proteins into smaller and soluble forms that include even the monomers...
  23. ncbi The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous recombination
    Yun Wu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 281:40001-9. 2006
    We examined the double-stranded DNA (dsDNA) binding preference of the Saccharomyces cerevisiae Rad52 protein and its homologue, the Rad59 protein...
  24. ncbi RPA mediates recombination repair during replication stress and is displaced from DNA by checkpoint signalling in human cells
    Kate M Sleeth
    The Institute for Cancer Studies, University of Sheffield, Sheffield, UK
    J Mol Biol 373:38-47. 2007
    ..We find that RPA is dispensable for checkpoint kinase 1 (Chk1) activation and that RPA directly binds RAD52 upon replication stress, suggesting a direct role in recombination repair...
  25. pmc Association between single-nucleotide polymorphisms in hormone metabolism and DNA repair genes and epithelial ovarian cancer: results from two Australian studies and an additional validation set
    Jonathan Beesley
    Queensland Institute of Medical Research, Herston, Queensland, Australia
    Cancer Epidemiol Biomarkers Prev 16:2557-65. 2007
    ..in steroid hormone synthesis (SRD5A2, CYP19A1, HSB17B1, and HSD17B4) and DNA repair (XRCC2, XRCC3, BRCA2, and RAD52) using two Australian ovarian cancer case-control studies, comprising a total of 1,466 cases and 1,821 controls of ..
  26. doi DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair
    Michael J McIlwraith
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Mol Cell 29:510-6. 2008
    ..strand of a D loop, leads to capture and annealing of the second end of a resected DSB in reactions mediated by RAD52 protein...
  27. pmc Identification of a second DNA binding site in the human Rad52 protein
    Wataru Kagawa
    RIKEN Systems and Structural Biology Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama, Japan
    J Biol Chem 283:24264-73. 2008
    b>Rad52 plays essential roles in homology-dependent double-strand break repair. Various studies have established the functions of Rad52 in Rad51-dependent and Rad51-independent repair processes...
  28. ncbi Genotype-phenotype relationship between DNA repair gene genetic polymorphisms and DNA repair capacity
    Aesun Shin
    Division of Cancer Prevention, National Cancer Control Research Institute, National Cancer Center, Goyang si, Gyeonggi Do, Korea
    Asian Pac J Cancer Prev 9:501-5. 2008
    ..repair genes included were those involved in double-strand break repair (ATM, XRCC2, XRCC4, XRCC6, LIG4, RAD51, RAD52), base excision repair (LIG1), nucleotide excision repair (ERCC1), and mismatch repair (hMLH1)...
  29. pmc Human Rad52-mediated homology search and annealing occurs by continuous interactions between overlapping nucleoprotein complexes
    Eli Rothenberg
    Howard Hughes Medical Institute and Center for the Physics of Living Cells, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 105:20274-9. 2008
    The Rad52 protein has critical functions in distinct pathways of the homology-directed DNA repair, one of which is to promote the annealing of complementary strands of DNA...
  30. ncbi RAD52 polymorphisms contribute to the development of papillary thyroid cancer susceptibility in Middle Eastern population
    A K Siraj
    Department of Human Cancer Genomics Research, Research Centre, KFNCCC and R, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
    J Endocrinol Invest 31:893-9. 2008
    ..homologous recombination pathway genes XRCC3 ARG94HIS and THR241MET, RAD51 UTR 15452658T>C, 15455419A>G, RAD52 2259 and GLN221GLU, conserved DNA damage response gene Tp53 PRO47SER, PRO72ARG, Tp53 UTR 7178189A>C and base ..
  31. pmc Human replication protein A-Rad52-single-stranded DNA complex: stoichiometry and evidence for strand transfer regulation by phosphorylation
    Xiaoyi Deng
    The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 987696 Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Biochemistry 48:6633-43. 2009
    ..b>Rad52 and RPA participate in the repair of double-stranded DNA breaks (DSBs)...
  32. pmc Human Rad52 binds and wraps single-stranded DNA and mediates annealing via two hRad52-ssDNA complexes
    Jill M Grimme
    Department of Biochemistry, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Nucleic Acids Res 38:2917-30. 2010
    b>Rad52 promotes the annealing of complementary strands of DNA bound by replication protein A (RPA) during discrete repair pathways...
  33. pmc Rad52 inactivation is synthetically lethal with BRCA2 deficiency
    Zhihui Feng
    Department of Radiation Oncology, Washington University, St Louis, MO 63108, USA
    Proc Natl Acad Sci U S A 108:686-91. 2011
    Synthetic lethality is a powerful approach to study selective cell killing based on genotype. We show that loss of Rad52 function is synthetically lethal with breast cancer 2, early onset (BRCA2) deficiency, whereas there was no impact ..
  34. pmc Influence of common genetic variation on lung cancer risk: meta-analysis of 14 900 cases and 29 485 controls
    Maria N Timofeeva
    International Agency for Research on Cancer, Lyon, France
    Hum Mol Genet 21:4980-95. 2012
    ..Several other genetic regions including variants of CHEK2 (22q12), TP53BP1 (15q15) and RAD52 (12p13) have been demonstrated to influence lung cancer risk in candidate- or pathway-based analyses...
  35. doi RAD52 inactivation is synthetically lethal with deficiencies in BRCA1 and PALB2 in addition to BRCA2 through RAD51-mediated homologous recombination
    B H Lok
    Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Oncogene 32:3552-8. 2013
    ..Previous work in our laboratory has shown that loss of RAD52 is synthetically lethal with BRCA2 deficiency, while exhibiting no impact on cell growth and viability in BRCA2-..
  36. pmc Personalized synthetic lethality induced by targeting RAD52 in leukemias identified by gene mutation and expression profile
    Kimberly Cramer-Morales
    Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Blood 122:1293-304. 2013
    ..HRR usually depends on BRCA1/2-RAD51, and RAD52-RAD51 serves as back-up...
  37. ncbi Heteroduplex formation by human Rad51 protein: effects of DNA end-structure, hRP-A and hRad52
    P Baumann
    Clare Hall Laboratories, Imperial Cancer Research Fund, South Mimms, Hertfordshire, EN6 3LD, UK
    J Mol Biol 291:363-74. 1999
    ..Joint molecule formation by hRad51 was stimulated or inhibited by hRad52, dependent upon the reaction conditions. The inhibitory effect could be overcome by the presence of hRP-A or excess heterologous DNA...
  38. ncbi Analysis of the human replication protein A:Rad52 complex: evidence for crosstalk between RPA32, RPA70, Rad52 and DNA
    Doba Jackson
    Department of Chemistry, University of Toledo, 2801 West Bancroft Street, OH 43606 3390, USA
    J Mol Biol 321:133-48. 2002
    ..b>Rad52 and RPA, along with other members of the Rad52 epistasis group of genes, repair double-stranded DNA breaks (DSBs)...
  39. doi Variants in DNA double-strand break repair and DNA damage-response genes and susceptibility to lung and head and neck cancers
    Patrick Danoy
    Centre National de Genotypage, Evry, France
    Int J Cancer 123:457-63. 2008
    ..Haplotype-analysis suggested potential associations (p < 0.05) between lung cancer and 2 genes (RECQL4 and RAD52), which came with q-value of 8%, and between H-N cancer and 1 gene (DNA-PK) but with q-value of 56%...
  40. ncbi Analysis of spontaneous and double-strand break-induced recombination in rad mutants of S. pombe
    E A Fortunato
    Department of Biology, University of California San Diego, La Jolla 92093 0322, USA
    Mutat Res 364:14-60. 1996
    ..In S. cerevisiae, the DSB/gap repair pathway is RAD52 dependent, and the RAD1 and RAD10 genes are involved in the SSA pathway...
  41. ncbi Cisplatin DNA cross-links do not inhibit S-phase and cause only a G2/M arrest in Saccharomyces cerevisiae
    K F Grossmann
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA
    Mutat Res 434:29-39. 1999
    ..to CDDP, as did controls with a defect in excision repair (rad1 and rad14) or a defect in recombination (rad51 and rad52)...
  42. pmc The role of DNA repair genes in recombination between repeated sequences in yeast
    B Liefshitz
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
    Genetics 140:1199-211. 1995
    ..The role of the RAD51, RAD52, RAD54, RAD55, and RAD57 genes, which are involved in recombinational repair, was investigated...
  43. pmc Specificities of the Saccharomyces cerevisiae rad6, rad18, and rad52 mutators exhibit different degrees of dependence on the REV3 gene product, a putative nonessential DNA polymerase
    H Roche
    Department of Microbiology, University of Manitoba, Winnipeg, Canada
    Genetics 140:443-56. 1995
    The Saccharomyces cerevisiae rad6, rad18, and rad52 mutants exhibit DNA repair deficiencies and distinct mutator phenotypes. DNA replication past unrepaired spontaneous damage might contribute to the specificities of these mutators...
  44. pmc The Sgs1 helicase of Saccharomyces cerevisiae inhibits retrotransposition of Ty1 multimeric arrays
    M Bryk
    Molecular Genetics Program, Wadsworth Center and School of Public Health, State University of New York at Albany, Albany, New York 12208, USA
    Mol Cell Biol 21:5374-88. 2001
    ..Formation of Ty1 multimers required the homologous recombination protein Rad52 but did not involve recombination between Ty1 cDNA and genomic Ty1 elements...
  45. ncbi Stability of YACs containing ribosomal or RCP/GCP locus DNA in wild-type S. cerevisiae and RAD mutant strains
    K Kohno
    Department of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan
    DNA Res 1:191-9. 1994
    ..5 rDNA tandem repeat units were transformed into hosts bearing lesions at the RAD1, RAD6, RAD51, or RAD52 loci...
  46. ncbi Involvement of SGS1 in DNA damage-induced heteroallelic recombination that requires RAD52 in Saccharomyces cerevisiae
    F Onoda
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Mol Gen Genet 264:702-8. 2001
    ..In this study, we found that SGS1 forms part of the RAD52 epistasis group when cells are exposed to MMS...
  47. pmc RAD50 and RAD51 define two pathways that collaborate to maintain telomeres in the absence of telomerase
    S Le
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genetics 152:143-52. 1999
    ..However, gene conversion mediated by the RAD52 pathway allows telomere lengthening in rare survivor cells...
  48. pmc Role of reciprocal exchange, one-ended invasion crossover and single-strand annealing on inverted and direct repeat recombination in yeast: different requirements for the RAD1, RAD10, and RAD52 genes
    F Prado
    , , Universidad de Sevilla, Spain
    Genetics 139:109-23. 1995
    ..Spontaneous and double-strand break (DSB)-induced deletions occur as RAD52-dependent and RAD52-independent events...
  49. pmc Repair of chromosome ends after telomere loss in Saccharomyces
    J L Mangahas
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 12:4078-89. 2001
    ..Both in a wild-type and a recombination deficient rad52 strain, most stabilization events were due to homologous recombination, whereas nonhomologous end joining was ..
  50. ncbi A Neurospora double-strand-break repair gene, mus-11, encodes a RAD52 homologue and is inducible by mutagens
    Y Sakuraba
    Department of Regulation Biology, Faculty of Science, Saitama University, Urawa, Japan
    Mol Gen Genet 264:392-401. 2000
    We isolated a Neurospora crassa cDNA that encodes a Rad52 homologue (ncRAD52) by PCR, using degenerate primers. RFLP mapping demonstrated that the cloned gene is located close to the ro-4 locus on the right arm of linkage group V (LGVR)...
  51. ncbi Therapeutic exploitation of tumor cell defects in homologous recombination
    Simon N Powell
    Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park, St Louis, MO 63108, USA
    Anticancer Agents Med Chem 8:448-60. 2008
    ..In the absence of BRCA2, the cell is more dependent on residual repair via Rad52, which makes Rad52 a target for therapy in BRCA-deficient tumors...
  52. pmc A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks
    P Schär
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, UK
    Genes Dev 11:1912-24. 1997
    ..marginally increased cellular sensitivity to several DNA damaging agents, and does not further sensitize cdc9 or rad52 mutant cells...
  53. ncbi Genotoxic and recombinogenic activities of the two beta-carboline alkaloids harman and harmine in Saccharomyces cerevisiae
    Jane Marlei Boeira
    Departamento de Biofisica e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, UFRGS, Av Bento Goncalves 9500, Prédio 43421, Campus do Vale, Caixa Postal 15005, CEP 91501 970, Porto Alegre, RS, Brazil
    Mutat Res 500:39-48. 2002
    ..in excision-resynthesis repair (rad3 and rad1), in error-prone repair (rad6) and in recombinational repair (rad52) showed enhanced sensitivity to harmine and harman, but the ranking of sensitivities was different for the two ..
  54. pmc Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA
    S L Hays
    Department of Biochemistry, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford University, Stanford, California 94305, USA
    Mol Cell Biol 18:4400-6. 1998
    ..a number of impaired recombination and repair phenotypes, all of which are suppressible by overexpression of RAD52. We demonstrate that a rad52 mutation is epistatic to the rfa1-44 mutation, placing RFA1 and RAD52 in the same ..
  55. pmc Different mating-type-regulated genes affect the DNA repair defects of Saccharomyces RAD51, RAD52 and RAD55 mutants
    Maria Valencia-Burton
    Department of Biology and Resenstiel Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 174:41-55. 2006
    ..deletions of RAD55 or RAD57, an ATPase-defective Rad51 mutation (rad51-K191R), and a C-terminal truncation of Rad52, rad52-Delta327...
  56. pmc Rsp5, a ubiquitin-protein ligase, is involved in degradation of the single-stranded-DNA binding protein rfa1 in Saccharomyces cerevisiae
    N Erdeniz
    Department of Genetics, College of Physicians and Surgeons, Columbia University, New York, New York 10032 2704, USA
    Mol Cell Biol 20:224-32. 2000
    In Saccharomyces cerevisiae, RAD1 and RAD52 are required for alternate pathways of mitotic recombination. Double-mutant strains exhibit a synergistic interaction that decreases direct repeat recombination rates dramatically...
  57. pmc Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes
    Zheng Dong
    Center for Immunology and Microbial Disease, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208 3479, USA
    Nucleic Acids Res 31:2576-85. 2003
    ..mechanisms, we determined whether spontaneous and DNA damage-associated SCE requires specific genes within the RAD52 and RAD3 epistasis groups in Saccharomyces cerevisiae strains containing two his3 fragments, his3-Delta5' and his3-..
  58. ncbi The HMG-domain protein Ixr1 blocks excision repair of cisplatin-DNA adducts in yeast
    M M McA'Nulty
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Mutat Res 362:75-86. 1996
    ..ixr1 strain is the result of enhanced repair was investigated in rad1, rad2, rad4, rad6, rad9, rad10, rad14 and rad52 backgrounds...
  59. pmc Genetic control of RNA polymerase I-stimulated recombination in yeast
    B R Zehfus
    Department of Biological Chemistry, Milton S Hershey Medical Center, Hershey, Pennsylvania 17033
    Genetics 126:41-52. 1990
    ..Mutations in RAD1 and RAD52 decrease the ability of HOT1 to stimulate intrachromosomal recombination while mutations in RAD4 and RAD50 do not ..
  60. ncbi Characterization of RAD52 homologs in the fission yeast Schizosaccharomyces pombe
    - van den Bosch M
    MGC Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands
    Mutat Res 461:311-23. 2001
    The RAD52 gene of Saccharomyces cerevisiae is essential for repair of DNA double-strand breaks (DSBs) by homologous recombination...
  61. ncbi The Escherichia coli RecA protein complements recombination defective phenotype of the Saccharomyces cerevisiae rad52 mutant cells
    Andrej Dudas
    Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 91 Bratislava 37, Slovak Republic
    Yeast 20:389-96. 2003
    The Saccharomyces cerevisiae rad52 mutants are sensitive to many DNA damaging agents, mainly to those that induce DNA double-strand breaks (DSBs). In the yeast, DSBs are repaired primarily by homologous recombination (HR)...
  62. ncbi The Saccharomyces cerevisiae checkpoint genes RAD9, CHK1 and PDS1 are required for elevated homologous recombination in a mec1 (ATR) hypomorphic mutant
    Michael Fasullo
    Ordway Research Institute, Albany, New York 12208, USA
    Cell Cycle 7:2418-26. 2008
    ..The mec1-21 hyper-recombination was partially reduced in rad9, pds1 and chk1 mutants, and abolished in rad52 mutants, suggesting the hyper-recombination results from RAD52-dependent recombination pathway(s) that require G(2)..
  63. doi Expression of double strand DNA breaks repair genes in pterygium
    Anna Lękawa-Ilczuk
    Department of Human Genetics, Medical University, Lublin, Poland
    Ophthalmic Genet 32:39-47. 2011
    ..This study was focused on the examination of the expression of genes and RAD50 protein taking part in homologous recombination...
  64. pmc Isolation and genetic analysis of extragenic suppressors of the hyper-deletion phenotype of the Saccharomyces cerevisiae hpr1 delta mutation
    H Santos-Rosa
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Spain
    Genetics 139:57-66. 1995
    ..characteristics we have found are the following: (1) one mutation, hrs1-1, reduces the frequency of deletions in rad52-1 strains 20-fold, suggesting that the HRS1 gene is involved in the formation of RAD52-independent deletions; (2) ..
  65. pmc Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis
    S L Gasior
    Department of Radiation and Cellular Oncology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 98:8411-8. 2001
    ..In meiosis, both Rad52 and Rad55/57 are required, whereas either Rad52 or Rad55/57 is sufficient to promote assembly of Rad51 in ..
  66. ncbi Further characterization of the yeast pso4-1 mutant: interaction with rad51 and rad52 mutants after photoinduced psoralen lesions
    M A de Morais
    Departamento de Biofisica e Centro de Biotecnologia, UFRGS, Av Bento Goncalves 9500, prédio 2A, B1 4, Campus do Vale, 91501 970 Porto Alegre, RS, Brasil
    Curr Genet 29:211-8. 1996
    ..The mode of interaction between pso4-1 and rad51 and between pso4-1 and rad52 mutants indicated that the PSO4 gene belongs to the RAD52 epistasis group for strand-break repair...
  67. ncbi Base analog 6-N-hydroxylaminopurine mutagenesis in the yeast Saccharomyces cerevisiae is controlled by replicative DNA polymerases
    P V Shcherbakova
    Department of Genetics, Sankt Petersburg University, Russia
    Mutat Res 369:33-44. 1996
    ..Null alleles of the PMS1, RAD6, REV3 and RAD52 genes did not affect HAP mutagenesis...
  68. pmc Homologous recombinational repair factors are recruited and loaded onto the viral DNA genome in Epstein-Barr virus replication compartments
    Ayumi Kudoh
    Division of Virology, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
    J Virol 83:6641-51. 2009
    ..we report that homologous recombinational repair (HRR) factors such as replication protein A (RPA), Rad51, and Rad52 as well as MRN complexes are recruited and loaded onto the newly synthesized viral genome in replication ..
  69. pmc Double-strand break repair pathways protect against CAG/CTG repeat expansions, contractions and repeat-mediated chromosomal fragility in Saccharomyces cerevisiae
    Rangapriya Sundararajan
    Program in Genetics, Sackler School of Biomedical Sciences, Tufts University, Boston, Massachusetts 02111, USA
    Genetics 184:65-77. 2010
    ..About two-thirds of the expansions that occurred in the absence of MRE11 were dependent on RAD52, implicating aberrant homologous recombination as a mechanism for generating expansions...
  70. pmc XRS2, a DNA repair gene of Saccharomyces cerevisiae, is needed for meiotic recombination
    E L Ivanov
    Institut Curie, Section de Biologie, Orsay, France
    Genetics 132:651-64. 1992
    ..The xrs2 mutation suppresses spore inviability of a spo13 rad52 strain suggesting that XRS2 acts prior to RAD52 in the meiotic recombination pathway...
  71. ncbi The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast
    Mika Yokoyama
    Institute for Virus Research, Kyoto University, Shogoinkawahara cho, Kyoto, Japan
    DNA Repair (Amst) 6:770-80. 2007
    ..Spontaneous Rad22 (recombination mediator protein RAD52 homolog) foci increase in S phase to late G2 phase in Deltamus7 and Deltamus81 cells...
  72. pmc Rad51-independent interchromosomal double-strand break repair by gene conversion requires Rad52 but not Rad55, Rad57, or Dmc1
    Thomas J Pohl
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Mol Cell Biol 28:897-906. 2008
    ..In yeast, HR is catalyzed by the Rad51 strand transferase and its "mediators," including the Rad52 single-strand DNA-annealing protein, two Rad51 paralogs (Rad55 and Rad57), and Rad54...
  73. ncbi Evidence that an endo-exonuclease controlled by the NUC2 gene functions in the induction of 'petite' mutations in Saccharomyces cerevisiae
    T Y Chow
    Department of Nuclear Medicine and Radiobiology, University of Sherbrooke, Quebec, Canada
    Curr Genet 20:39-44. 1991
    Defects in the RAD52 gene of the yeast Saccharomyces cerevisiae reduce the levels of the NUC2 endo-exonuclease by approximately 90% compared to the levels in wild-type strains...
  74. ncbi A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae
    Y Bai
    Columbia University College of Physicians and Surgeons, Department of Microbiology and Institute of Cancer Research, New York, New York 10032, USA
    Genes Dev 10:2025-37. 1996
    ..as a recombination reporter we have previously shown that mitotic recombination is dependent on the RAD52 gene...
  75. pmc Distinct RAD51 associations with RAD52 and BCCIP in response to DNA damage and replication stress
    Justin Wray
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Cancer Research and Treatment Center, Albuquerque, New Mexico, USA
    Cancer Res 68:2699-707. 2008
    ..In yeast, Rad51 function is facilitated by Rad52 and other "mediators...
  76. pmc Regulation of Rad51 recombinase presynaptic filament assembly via interactions with the Rad52 mediator and the Srs2 anti-recombinase
    Changhyun Seong
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 284:24363-71. 2009
    ..The Rad51 recombinase, a member of the RAD52 group of recombination proteins, catalyzes the homologous recombination reaction in the context of a helical ..
  77. pmc Rad1, rad10 and rad52 mutations reduce the increase of microhomology length during radiation-induced microhomology-mediated illegitimate recombination in saccharomyces cerevisiae
    Cecilia Y Chan
    Departments of Pathology, Environmental Health and Radiation Oncology, Geffen School of Medicine and School of Public Health, UCLA, Los Angeles, California 90095, USA
    Radiat Res 172:141-51. 2009
    ..Here we show that radiation-induced microhomology-mediated recombination is reduced by deletion of RAD52, RAD1 and RAD10 but is not affected by deletion of RAD51 and RAD2...
  78. doi Recovery of deficient homologous recombination in Brca2-depleted mouse cells by wild-type Rad51 expression
    Shauna A Lee
    Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1 Canada
    DNA Repair (Amst) 8:170-81. 2009
    ..the roles of BRCA2 in regulating RAD51 and how other proteins implicated in RAD51 regulation, such as RAD52 and RAD54 function relative to BRCA2 is not known...
  79. pmc Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination
    J P McDonald
    Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York 10032
    Genetics 137:393-405. 1994
    ..Unrepaired heteroduplex increases significantly only in rad52 mutant strains...
  80. pmc Detection of loss of heterozygosity at RAD51, RAD52, RAD54 and BRCA1 and BRCA2 loci in breast cancer: pathological correlations
    R Gonzalez
    Department of Medical Oncology, Clinica Puerta de Hierro, Madrid, Spain
    Br J Cancer 81:503-9. 1999
    ..1, 12p13, 1p32, 17q21 and 13q12-13 regions may collaborate in the inactivation of RAD51, RAD52, RAD54, BRCA1, BRCA2 and possibly other genes implicated in the repair of double-stranded DNA and in DNA ..
  81. pmc SUMO modification of Rad22, the Schizosaccharomyces pombe homologue of the recombination protein Rad52
    J C Ho
    Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
    Nucleic Acids Res 29:4179-86. 2001
    ..response, which may be modified by Pmt3 and have identified Rad22, the fission yeast homologue of the recombination protein Rad52. Purification of myc + His-tagged Rad22 protein from cells expressing HA-tagged Pmt3 identifies an 83 ..
  82. ncbi Heteroduplex joint formation by a stoichiometric complex of Rad51 and Rad52 of Saccharomyces cerevisiae
    Naoto Arai
    Department of Applied Biological Science, Nihon University College of Bioresource Sciences, Fujisawa shi, Kanagawa, Japan
    J Biol Chem 280:32218-29. 2005
    Both Rad51 and Rad52 are required for homologous genetic recombination in Saccharomyces cerevisiae. Rad51 promotes heteroduplex joint formation, a general step in homologous recombination...
  83. ncbi Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31
    Niloofar Davoodi Vijeh Motlagh
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Sendai, Miyagi 980 8578, Japan
    DNA Repair (Amst) 5:1459-74. 2006
    ..Deletion of SGS1, RAD52, SRS2, MRC1 or RAD24 had a deleterious effect only in combination with those pol31 alleles that had a phenotype as ..
  84. pmc Loss and fragmentation of chromosome 5 are major events linked to the adaptation of rad52-DeltaDelta strains of Candida albicans to sorbose
    Encarnación Andaluz
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Fungal Genet Biol 44:789-98. 2007
    Candida albicans can adapt and grow on sorbose plates by losing one copy of Chr5. Since rad52 mutants of Saccharomyces cerevisiae lose chromosomes at a high rate, we have investigated the ability of C. albicans rad52 to adapt to sorbose...
  85. ncbi Error-free RAD52 pathway and error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiae
    Kingo Endo
    Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    Genes Genet Syst 82:35-42. 2007
    ..Using the CAN1 gene in haploid cells or heterozygous diploid cells, we characterized the effects of mutations in the RAD52 and REV3 genes of Saccharomyces cerevisiae in spontaneous mutagenesis...
  86. ncbi Homologous recombination and the yKu70/80 complex exert opposite roles in resistance against the killer toxin from Pichia acaciae
    Roland Klassen
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, D 48149 Munster, Germany
    DNA Repair (Amst) 6:1864-75. 2007
    ..e. histone H2A phosphorylation and formation of subnuclear repair foci by GFP tagged recombination protein Rad52. As only moderate chromosome fragmentation could be detected by PFGE, transient occurrence and ..
  87. pmc Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators
    Yumiko Kurokawa
    Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Kanagawa, Japan
    PLoS Biol 6:e88. 2008
    In the fission yeast Schizosaccharomyces pombe, genetic evidence suggests that two mediators, Rad22 (the S. pombe Rad52 homolog) and the Swi5-Sfr1 complex, participate in a common pathway of Rhp51 (the S...
  88. pmc Telomerase deficiency affects the formation of chromosomal translocations by homologous recombination in Saccharomyces cerevisiae
    Damon H Meyer
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California, USA
    PLoS ONE 3:e3318. 2008
    ..This decrease correlated with a sequestration of the central homologous recombination factor, Rad52, to telomeres determined by chromatin immuno-precipitation...
  89. doi RAD51 loss of function abolishes gene targeting and de-represses illegitimate integration in the moss Physcomitrella patens
    D G Schaefer
    Institut Jean Pierre Bourgin, Station de Genetique et d Amelioration des plantes, UR254, INRA, Route de St Cyr, 78026 Versailles, France
    DNA Repair (Amst) 9:526-33. 2010
    ..Different mechanisms of HR have been described which depend on the Rad52 epistasis group of genes, but which specific mechanism is used by the cell for GT remains unclear...
  90. doi The putative nuclear localization signal of the human RAD52 protein is a potential sumoylation site
    Kengo Saito
    Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    J Biochem 147:833-42. 2010
    b>RAD52, a key factor in homologous recombination (HR), plays important roles in both RAD51-dependent and -independent HR pathways...
  91. ncbi DNA photodamage, repair, gene induction and genotoxicity following exposures to 254 nm UV and 8-methoxypsoralen plus UVA in a eukaryotic cell system
    D Averbeck
    Institut Curie Section de Recherche, Paris, France
    Photochem Photobiol 68:289-95. 1998
    ..of the DNA repair gene RAD51 in RAD51-LACZ fusion strains using the dsb repair and recombination deficient mutant rad52 and the corresponding wild type, and we determined the formation of dsb by pulsed-field gel electrophoresis...
  92. pmc Mechanisms of Rad52-independent spontaneous and UV-induced mitotic recombination in Saccharomyces cerevisiae
    Eric Coïc
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Genetics 179:199-211. 2008
    ..In rad52 strains, gene conversion is reduced 75-fold and the majority of His+ recombinants are crossover associated, with ..
  93. pmc Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae
    E L Ivanov
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254 9110
    Mol Cell Biol 14:3414-25. 1994
    In Saccharomyces cerevisiae, a large number of genes in the RAD52 epistasis group has been implicated in the repair of chromosomal double-strand breaks and in both mitotic and meiotic homologous recombination...
  94. pmc Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae
    Y Tsukamoto
    Department of Molecular Biology, Univesity of Tokyo, Japan
    Genetics 142:383-91. 1996
    ..of several rad mutations showed that the recombination rate was reduced by 30-, 10-, 10-, and 10-fold in the rad52, rad50, mre11, and xrs2 mutants, respectively, while in the rad51, 54, 55, and 57 mutants, the rate was comparable ..
  95. pmc SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae
    P M Watt
    Imperial Cancer Research Fund Laboratories, University of Oxford, John Radcliffe Hospital, United Kingdom
    Genetics 144:935-45. 1996
    ..Hyperrecombination was partially independent of both RAD52 and RAD1...
  96. ncbi Mutations in the RAD54 recombination gene in primary cancers
    M Matsuda
    Department of Molecular Pathology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Japan
    Oncogene 18:3427-30. 1999
    ..Since RAD51 forms a complex with other members of the RAD52 epistasis group and with BRCA proteins, it is reasonable to ask if alterations of members of the RAD52 epistasis ..
  97. pmc DNA damage-inducible and RAD52-independent repair of DNA double-strand breaks in Saccharomyces cerevisiae
    C W Moore
    Department of Microbiology and Immunology, City University of New York Medical School Sophie Davis School of Biomedical Education and Graduate Programs in Biochemistry and Biology, New York, New York 10031, USA
    Genetics 154:1085-99. 2000
    Chromosomal repair was studied in stationary-phase Saccharomyces cerevisiae, including rad52/rad52 mutant strains deficient in repairing double-strand breaks (DSBs) by homologous recombination...
  98. pmc Replication protein A is required for meiotic recombination in Saccharomyces cerevisiae
    Christine Soustelle
    Institut Curie, Section de Recherche, CNRS UMR144, 75248 Paris Cedex 05, France
    Genetics 161:535-47. 2002
    ..DSBs undergo a 5' --> 3' resection to produce 3' single-stranded DNA ends that serve to channel DSBs into the RAD52 recombinational repair pathway...
  99. ncbi Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre
    Michael Lisby
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032 2704, USA
    Nat Cell Biol 5:572-7. 2003
    ..Using this system, we demonstrate for the first time that Rad52 DNA repair foci and DSBs colocalize...
  100. ncbi In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination
    Neal Sugawara
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 12:209-19. 2003
    ....
  101. pmc Increase in Ty1 cDNA recombination in yeast sir4 mutant strains at high temperature
    Sarah J Radford
    Department of Biology, Juniata College, Huntingdon, Pennsylvania 16652, USA
    Genetics 168:89-101. 2004
    ..the apparent increase in transposition activity in sir4 mutant strains at high temperature is dependent on the RAD52 gene and is thus likely resulting from an increase in Ty1 cDNA recombination, rather than in IN-mediated ..

Research Grants45

  1. ROLES OF HUMAN RECOMBINATION GENES IN GENOME INSTABILITY
    David Chen; Fiscal Year: 2001
    ..In the yeast S erevisiae, there is strong evidence that genes of the RAD52 epistasis group, which are essential for DNA double-stand break repair and homologous recombination, are also ..
  2. Patrick Sung; Fiscal Year: 2016
    ..Proteins encoded by evolutionarily conserved genes of the RAD52 epistasis group catalyze the HR reaction...
  3. Simon N Powell; Fiscal Year: 2016
    ..The Rad52 protein is redundant for homologous recombination in mammalian cells, but in the absence of a functioning BRCA1-..
  4. Recombination and fork progression in bacteriophage T4
    Stephen W White; Fiscal Year: 2013
    ..UvsW mediate the core of the homologous recombination reaction and are related to the eukaryotic proteins Rad51, Rad52 and Rad54, respectively...
  5. Role of RPA Hyperphosphorylation in DNA Damage Responses
    MOISES ALEJANDRO SERRANO; Fiscal Year: 2012
    ..1) the role of hyperphosphorylated RPA in the suppression of origin firing;2) the interaction between Rad51/Rad52 and RPA and its effect by RPA hyperphosphorylatlon;and 3) the cellular molecular interactions stimulated and/or ..
  6. Development of RAD52 Inhibitors to Induce Lethality of BRCA2-Deficient Cells
    Alexander V Mazin; Fiscal Year: 2013
    ..In yeast, Rad52 protein plays a key role in HR...
  7. RODNEY J ROTHSTEIN; Fiscal Year: 2014
    ..The specific aims of this proposal are focused on the following three subjects: (1) The Rad52 DNA repair protein: We will continue our molecular genetic characterization of the Rad52 DNA repair pathway using ..
  8. WILLIAM GAINES; Fiscal Year: 2014
    ..Proteins encoded by evolutionarily conserved genes of the RAD52 epistasis group catalyze the HR reaction...
  9. Philip A Hieter; Fiscal Year: 2016
    ..Five subunits of the mRNA cleavage and polyadenylation factor (CPF) exhibit CIN and high rates of spontaneous Rad52-foci while splicing factors show wild-type levels of damage...
  10. GENETIC AND PHYSICAL ANALYSIS OF RECOMBINATION REPAIR
    Jac Nickoloff; Fiscal Year: 2002
    ..We will employ both knock-out and overexpression strategies to investigate the roles of XRS2, RAD51, and RAD52 in recombinational repair...
  11. ROLES OF UBIQUITIN CONJUGATING ENZYMES IN DNA REPAIR
    Zhiyuan Shen; Fiscal Year: 2001
    The yeast RAD52 epistasis group of genes are involved in DNA recombination and repair of DNA damaged by radiation and other environmental agents such as cross-linking and alkylating agents...
  12. Repair of DNA Ends Following Damage in vivo
    Justin Courcelle; Fiscal Year: 2009
    ..coli belongs to a ubiquitous group of recombination mediators (RMs) that include eukaryotic proteins such as Rad52 and BRCA2 and are required to maintain genome stability in the presence of DNA damage...
  13. RAD52 FUNCTION IN MAMMALIAN SOMATIC CELLS
    Colin Campbell; Fiscal Year: 2001
    ..Adapted from the Investigator's Abstract) The objective of this proposal is to determine the function of RAD52 in mammalian somatic cells...
  14. Role of Rad51 Paralogs in Mammalian DNA Repair
    Patrick Sung; Fiscal Year: 2006
    ..and proteins that function in concert to mediated homologous recombination are collectively referred to as the RAD52 epistasis group...
  15. ANALYSIS OF LARGE DNA PALINDROME FORMATION IN YEAST
    David Butler; Fiscal Year: 2000
    ..A mutation in RAD52, a gene required for repair of DNA double-strand breaks (DSBs) by homologous recombination, blocks palindrome ..
  16. GENETIC CONTROL OF IMMUNOGLOBULIN VARIABLE REGIONS
    Katherine Knight; Fiscal Year: 2002
    ..molecules involved in somatic gene conversion by cloning and characterizing the rabbit homologues of yeast RAD52 epistasis genes, genes known to be involved in gene conversion...
  17. The Genetics of Gene Targeting in Vertebrate Cells
    Matthew Porteus; Fiscal Year: 2006
    ..include understanding: 1) the cell cycle regulation of GT; 2) the rate of GT in DT40 cells; 3) the role of Rad52 in inhibiting GT and; 4) the rate of GT in cells with mutations involved in DNA double-stranded break repair...
  18. RODNEY J ROTHSTEIN; Fiscal Year: 2016
    ..We will use yeast as outlined in the specific aims: (1) The Rad52 DNA repair protein: We will continue our molecular genetic characterization of the Rad52 DNA repair pathway using ..
  19. Transcription-enhanced recombination in mammalian cells
    Zhiyuan Shen; Fiscal Year: 2006
    ..Current models suggest that binding of Ku proteins to DNA ends initiates NHEJ whereas binding of RAD52 solicits HRR, and that HRR is more frequently associated with replication or transcriptionally active loci...
  20. Role of Human Rad52 and Rad51- paralogs in DNA Repair
    KENDALL KNIGHT; Fiscal Year: 2007
    ..This study focuses on the functional organization of the human Rad52 protein (HsRad52) as well as the functional relationships between HsRad52 and other human recombination proteins...
  21. A Tripartite Approach to Elucidate Rdh54 function
    Rebecca Burgess; Fiscal Year: 2007
    Members of the RAD52 epistasis group are necessary for maintaining genomic integrity. Through homologous recombination (HR), RAD52 group proteins faithfully repair lesions caused by spontaneous or induced DNA damage...
  22. Regulation of Double Strand Break Repair
    JACQUELINE BARLOW; Fiscal Year: 2007
    ..DESCRIPTION (provided by applicant): In response to DNA damage causing double strand breaks (DSBs), the recombination protein Rad52 forms discrete nuclear foci after the initiation of DNA replication and S phase entry...
  23. Mechanisms of Cellular Transduction with AAV Vectors
    Daniel Miller; Fiscal Year: 2006
    ..Finally, we plan to disrupt genes involved in homologous recombination and DSBR (Rad52, Rad54, and Ku70) using homologous integration of AAV vectors and define cellular pathways involved in homologous ..
  24. NMR STUDIES OF PROTEIN INTERACTIONS IN UBIQUITINATION
    Yuan Chen; Fiscal Year: 2002
    ..of a ubiquitin conjugation enzyme UBC9 (E2) with UBL-1 (a ubiquitin homologue) and target proteins Rad51, Rad52 and p53. We will characterize the UBL1- UBC9 conjugate and its interactions with target proteins...
  25. RECOMBINATION ASSOCIATED WITH YEAST RETROTRANSPOSONS
    Susan Liebman; Fiscal Year: 1990
    ..The effect of the recombination deficient mutation, rad52-, on the types of recombination that occur between repeated Tys, deltas, and non-transposable sequences will be ..
  26. Genetic Recombination/Genomic Rearrangements Conference
    LORRAINE SYMINGTON; Fiscal Year: 2005
    ..abstract_text> ..
  27. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    Michael Fasullo; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  28. RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEAST
    Michael Fasullo; Fiscal Year: 2004
    ..abstract_text> ..
  29. Mechanisms of Double-strand Break Repair in Yeast
    LORRAINE SYMINGTON; Fiscal Year: 2009
    ..b>Rad52, Rad54, Rad55 and Rad57 function in formation and/or stabilization of the Rad51 nucleoprotein filament, whereas ..
  30. Genetic Recombination and Chromosome Rearrangements
    LORRAINE SYMINGTON; Fiscal Year: 2007
    ..Much of the conference will be devoted to topics of concern both to the National Cancer Institute and to the Institute of Environmental and Health Sciences. [unreadable] [unreadable] [unreadable] [unreadable]..
  31. Structure-functional studies of recombination/replication mediator proteins
    Sergey Korolev; Fiscal Year: 2010
    ..It will help formulate future studies of similar processes in eukaryotes where their malfunctions are highly associated with tumorigenesis. ..
  32. SYNTHESIS OF BLEOMYCIN GROUP ANTIBIOTICS AND ANALOGUES
    SIDNEY HECHT; Fiscal Year: 2007
    ..These libraries will be used to select BLMs having specific desirable properties and the identified analogues will be characterized thoroughly as potential anti-tumor agents. ..
  33. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2004
    ..This assay, in vitro biochemical assays, and cellular responses to chemical mutagens will evaluate the hypothesis that ORF YMR156c is the 3' phosphatase portion of yeast polynucleotide kinase. ..
  34. MECHANISM AND INHIBITION OF HUMAN DNA TOPOISOMERASE I
    SIDNEY HECHT; Fiscal Year: 2003
    ..abstract_text> ..
  35. MECHANISM OF INDUCTION OF MALIGNANT GLIOMAS
    Demetrius Kokkinakis; Fiscal Year: 2002
    ..Genetic differences between tumor cells and those capable of infiltrating the normal parenchyma will also be identified and compared to those of initiated multipotent progenitor cells in order to understand their lineage. ..
  36. Roles of BRCA1, BRCA2 in Homologous Recombination
    Simon Powell; Fiscal Year: 2008
    ..Ultimately, the defects in HR that promote carcinogenesis by causing genetic instability may also offer a novel therapeutic target against the cancers that arise in this setting. ..
  37. NATURAL PRODUCTS DISCOVERY - NUCLEAR AND SIGNALING TARGE
    SIDNEY HECHT; Fiscal Year: 2004
    ..preparing in quantity and evaluating promising compounds in tumor cells (for mechanism, biochemical pharmacology, interaction with known antineoplastic agents) and in animal tumor models (Programs 1-3) ..
  38. Radiation Oncology 2008 Gordon Research Conference
    Simon Powell; Fiscal Year: 2008
    ..Improving the effectiveness of radiation therapy, which is used in the treatment of 50% of all human cancers, will have a major impact on current approaches to treatment. [unreadable] [unreadable] [unreadable] [unreadable]..
  39. ELABORATION OF MODIFIED PROTEINS USING MISACYLATED TRNAS
    SIDNEY HECHT; Fiscal Year: 2007
    ..Also of interest is the exploitation of a new method for introducing a broader range of amino acid analogues into the derived proteins, and the elaboration of the modified proteins in an intact cellular system ..
  40. Alternate Spliced Repair Transcripts & Genome Stability
    Rodney Rothstein; Fiscal Year: 2006
    ..In addition, the approach outlined here is applicable to other tissue types and it is anticipated that this research will lead to further study of other human tumors. ..
  41. INTERACTION OF BLEOMYCIN WITH RNA AND DNA
    SIDNEY HECHT; Fiscal Year: 2006
    ..In spite of the obvious utility and importance of the bleomycins as antitumor agents, there are clear opportunities to alter the molecular behavior of bleomycin, and thereby potentially improve its antitumor efficacy. ..
  42. GENOME STABILITY IN RAD MUTANTS
    ADAM BAILIS; Fiscal Year: 2006
    ..This combination of genetic and physical methods will promote a more thorough understanding of several of the molecular interactions critical for SSR, and the maintenance of genome stability. ..
  43. Estrogen Bioactivation and Risk of Ovarian Cancer
    Thomas Sellers; Fiscal Year: 2005
    ..It is innovative, plausible, and important to evaluate similar associations with epithelial ovarian cancer. The results could have profound implications for our understanding of the disease. ..
  44. Mechanisms of Nonhomologous Repair of Damaged DNA
    Sang Eun Lee; Fiscal Year: 2005
    ..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..
  45. New Methods for Using Gene Disruption Libraries
    Rodney Rothstein; Fiscal Year: 2004
    ..g., Candida or one of many bacterial strains). Successful completion of these aims will greatly expand the methods available in the molecular geneticist's tool kit. ..