RAD51

Summary

Gene Symbol: RAD51
Description: RAD51 recombinase
Alias: BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA, DNA repair protein RAD51 homolog 1, BRCA1/BRCA2-containing complex, subunit 5, RAD51 homolog A, RecA, E. coli, homolog of, RecA-like protein, recombination protein A
Species: human

Top Publications

  1. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000
  2. ncbi Insights into DNA recombination from the structure of a RAD51-BRCA2 complex
    Luca Pellegrini
    University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1GA, UK
    Nature 420:287-93. 2002
  3. doi Single nucleotide polymorphisms of RAD51 G135C, XRCC2 Arg188His and XRCC3 Thr241Met homologous recombination repair genes and the risk of sporadic endometrial cancer in Polish women
    Hanna Romanowicz-Makowska
    Laboratory of Molecular Genetics, Department of Pathology, Medical University of Lodz, Lodz, Poland
    J Obstet Gynaecol Res 38:918-24. 2012
  4. ncbi Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro
    P Baumann
    Imperial Cancer Research Fund, South Mimms, Herts, United Kingdom
    Cell 87:757-66. 1996
  5. pmc Counting RAD51 proteins disassembling from nucleoprotein filaments under tension
    Joost van Mameren
    Laser Centre and Department of Physics and Astronomy, VU University, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands
    Nature 457:745-8. 2009
  6. ncbi Potential role for the BLM helicase in recombinational repair via a conserved interaction with RAD51
    L Wu
    Imperial Cancer Research Fund Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    J Biol Chem 276:19375-81. 2001
  7. ncbi T-antigen of the human polyomavirus JC attenuates faithful DNA repair by forcing nuclear interaction between IRS-1 and Rad51
    Joanna Trojanek
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Biology Life Science Building, Philadelphia, Pennsylvania 19122, USA
    J Cell Physiol 206:35-46. 2006
  8. ncbi S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2414-20. 2002
  9. ncbi RAD51 homologous recombination repair gene haplotypes and risk of acute myeloid leukaemia
    Sara Rollinson
    Division of Laboratory and Regenerative Medicine, Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    Leuk Res 31:169-74. 2007
  10. doi RAD51 G135C polymorphism is associated with breast cancer susceptibility: a meta-analysis involving 22,399 subjects
    Haiming Sun
    Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin 150081, China
    Breast Cancer Res Treat 125:157-61. 2011

Research Grants

  1. Michael M Cox; Fiscal Year: 2014
  2. Timothy M Lohman; Fiscal Year: 2016
  3. UNDERSTANDING HOMOLOGY IN GENETIC RECOMBINATION
    Scott F Singleton; Fiscal Year: 2012
  4. Recombination and fork progression in bacteriophage T4
    Stephen W White; Fiscal Year: 2013
  5. The MRAD9 Radioresistance Gene
    Howard B Lieberman; Fiscal Year: 2013
  6. The Novel Role of Branch Migration Protein Rad54 in Maintaining Genome Stability
    MATTHEW JOHN ROSSI; Fiscal Year: 2011
  7. Patrick Sung; Fiscal Year: 2016
  8. MECHANISTIC STUDIES OF GENETIC RECOMBINATION
    STEPHEN KOWALCZYKOWSKI; Fiscal Year: 1999
  9. DNA HELICASES IN RECOMBINATION AND REPAIR
    Hannah L Klein; Fiscal Year: 2010
  10. Simon N Powell; Fiscal Year: 2016

Detail Information

Publications306 found, 100 shown here

  1. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000
    ..Recently it has been reported that a member of the histone H2A family, H2AX, becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites...
  2. ncbi Insights into DNA recombination from the structure of a RAD51-BRCA2 complex
    Luca Pellegrini
    University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1GA, UK
    Nature 420:287-93. 2002
    The breast cancer susceptibility protein BRCA2 controls the function of RAD51, a recombinase enzyme, in pathways for DNA repair by homologous recombination...
  3. doi Single nucleotide polymorphisms of RAD51 G135C, XRCC2 Arg188His and XRCC3 Thr241Met homologous recombination repair genes and the risk of sporadic endometrial cancer in Polish women
    Hanna Romanowicz-Makowska
    Laboratory of Molecular Genetics, Department of Pathology, Medical University of Lodz, Lodz, Poland
    J Obstet Gynaecol Res 38:918-24. 2012
    The genes RAD51, XRCC2 and XRCC3 encode proteins that are important for the repair of double-strand DNA breaks by homologous recombination...
  4. ncbi Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro
    P Baumann
    Imperial Cancer Research Fund, South Mimms, Herts, United Kingdom
    Cell 87:757-66. 1996
    ..Using circular ssDNA and linear dsDNA (3.0 kb in length), we demonstrate that hRad51 promotes homologous pairing and strand exchange reactions in vitro...
  5. pmc Counting RAD51 proteins disassembling from nucleoprotein filaments under tension
    Joost van Mameren
    Laser Centre and Department of Physics and Astronomy, VU University, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands
    Nature 457:745-8. 2009
    The central catalyst in eukaryotic ATP-dependent homologous recombination consists of RAD51 proteins, polymerized around single-stranded DNA. This nucleoprotein filament recognizes and invades a homologous duplex DNA segment...
  6. ncbi Potential role for the BLM helicase in recombinational repair via a conserved interaction with RAD51
    L Wu
    Imperial Cancer Research Fund Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    J Biol Chem 276:19375-81. 2001
    ..In eukaryotes, a central step in homologous recombination is catalyzed by the RAD51 protein...
  7. ncbi T-antigen of the human polyomavirus JC attenuates faithful DNA repair by forcing nuclear interaction between IRS-1 and Rad51
    Joanna Trojanek
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Biology Life Science Building, Philadelphia, Pennsylvania 19122, USA
    J Cell Physiol 206:35-46. 2006
    ..Following T-antigen-mediated nuclear translocation, IRS-1 binds Rad51 at the site of damaged DNA...
  8. ncbi S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2414-20. 2002
    ..Monoubiquitinated FANCD2 colocalizes with BRCA1 and RAD51 in S-phase-specific nuclear foci...
  9. ncbi RAD51 homologous recombination repair gene haplotypes and risk of acute myeloid leukaemia
    Sara Rollinson
    Division of Laboratory and Regenerative Medicine, Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    Leuk Res 31:169-74. 2007
    ..leukaemia (AML) risk, we have examined the frequency of two variants in the 5' untranslated region (UTR) of RAD51 (RAD51 135 G>C and the RAD51 172 G>T) in a large case-control study of acute myeloid leukaemia (AML)...
  10. doi RAD51 G135C polymorphism is associated with breast cancer susceptibility: a meta-analysis involving 22,399 subjects
    Haiming Sun
    Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin 150081, China
    Breast Cancer Res Treat 125:157-61. 2011
    Several studies have investigated the associations between RAD51 G135C polymorphism and the susceptibility to breast cancer, but results have been inconclusive...
  11. pmc Activation of HIV-1 LTR by Rad51 in microglial cells
    Inna Rom
    Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, Philadelphia, PA, USA
    Cell Cycle 9:3715-22. 2010
    ..Here we demonstrate that expression of Rad51, a major component of the homologous recombination-directed DNA repair (HRR) pathway, is induced upon HIV-1 ..
  12. doi RAD51 and XRCC3 gene polymorphisms and the risk of developing acute myeloid leukemia
    Mona S Hamdy
    Department of Clinical and Chemical Pathology, Faculty of Medicine, El Kasr El Aini Hospital, Cairo, Egypt
    J Investig Med 59:1124-30. 2011
    b>RAD51 (Rec A homolog of E. coli) is a polymorphic gene and one of the central proteins in homologous recombination-DNA-double-stand breaks (HR-DNA-DSB) repair pathway, which is vital in maintaining genetic stability within a cell...
  13. doi RAD51 overexpression is a negative prognostic marker for colorectal adenocarcinoma
    Pierre Tennstedt
    Institute of Pathology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Int J Cancer 132:2118-26. 2013
    b>RAD51 is the central protein in the homologous recombination pathway and is therefore of great relevance in terms of both therapy resistance as well as genomic stability...
  14. ncbi RAD51 genotype and triple-negative breast cancer (TNBC) risk in Polish women
    Beata Smolarz
    Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish Mother s Memorial Hospital, Lodz, Poland
    Pol J Pathol 64:39-43. 2013
    ..The aim of this work was to evaluate the single nucleotide polymorphism -135G>C (rs1801320) of the RAD51 gene encoding DNA repair proteins by homologous recombination (HR) in triple-negative breast cancer (TNBC)...
  15. pmc Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes
    O V Kovalenko
    Department of Genetics, Yale University School of Medicine, New Haven CT 06510, USA
    Proc Natl Acad Sci U S A 93:2958-63. 1996
    ..According to the Ga14 two-hybrid system analysis, HsUbc9 protein interacts with human recombination protein Rad51. A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein...
  16. ncbi Characterisation of the promoter region of the human DNA-repair gene Rad51
    L Hasselbach
    Institute of Pathology, University Clinic Schleswig Holstein, Campus Luebeck, Germany
    Eur J Gynaecol Oncol 26:589-98. 2005
    Regulatory elements of the 5'-flanking region of the DNA-repair gene Rad51 were analysed to characterise pathological alterations of Rad51 mRNA expression during tumour development.
  17. pmc Polymorphisms in RAD51, XRCC2 and XRCC3 genes of the homologous recombination repair in colorectal cancer--a case control study
    Renata Krupa
    Laboratory of DNA Repair, Department of Molecular Genetics, University of Lodz, and Department of Surgical Oncology, N Copernicus Hospital, Banacha 12 16, 90 237, Lodz, Poland
    Mol Biol Rep 38:2849-54. 2011
    XRCC2 and XRCC3 proteins are structurally and functionally related to RAD51 which play an important role in the homologous recombination, the process frequently involved in cancer transformation...
  18. doi DNA repair gene expression and risk of locoregional relapse in breast cancer patients
    Romuald Le Scodan
    Department of Radiation Oncology, Centre Rene Huguenin, Saint Cloud, France
    Int J Radiat Oncol Biol Phys 78:328-36. 2010
    ..Radiation therapy appears to kill cells mainly by inducing DNA double-strand breaks. We investigated whether the DNA repair gene expression status might influence the risk of locoregional recurrence (LRR) in breast cancer patients...
  19. doi The importance of XRCC2 in RAD51-related DNA damage repair
    Cathryn E Tambini
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxon OX11 0RD, United Kingdom
    DNA Repair (Amst) 9:517-25. 2010
    ..The central HR protein is RAD51, which ensures high fidelity DNA repair by facilitating strand exchange between damaged and undamaged homologous ..
  20. ncbi BCR/ABL regulates mammalian RecA homologs, resulting in drug resistance
    A Slupianek
    Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA
    Mol Cell 8:795-806. 2001
    b>RAD51 is one of six mitotic human homologs of the E. coli RecA protein (RAD51-Paralogs) that play a central role in homologous recombination and repair of DNA double-strand breaks (DSBs)...
  21. pmc The carboxyl terminus of Brca2 links the disassembly of Rad51 complexes to mitotic entry
    Nabieh Ayoub
    Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK
    Curr Biol 19:1075-85. 2009
    The Rad51 recombinase assembles on DNA to execute homologous DNA recombination (HR)...
  22. ncbi Identification of Rad51 alteration in patients with bilateral breast cancer
    M Kato
    Department of Molecular Diagnosis, Cancer Institute, Tokyo, Japan
    J Hum Genet 45:133-7. 2000
    The human Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair...
  23. ncbi Elevated levels of Rad51 recombination protein in tumor cells
    Elke Raderschall
    Max Planck Institute of Molecular Genetics, 14195 Berlin, Germany
    Cancer Res 62:219-25. 2002
    b>Rad51 is the key enzyme for homologous recombination, an evolutionarily conserved mechanism for the repair of DNA damage and the generation of genetic diversity...
  24. ncbi Polymorphisms of DNA repair genes and risk of glioma
    Li E Wang
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 64:5560-3. 2004
    ..genetic variation in genes involved in DNA strand breaks, such as XRCC1 in single-strand break repair, XRCC3 and RAD51 in homologous recombination repair, and XRCC7 in nonhomologous end joining double-strand break repair...
  25. pmc Overexpression of RAD51 occurs in aggressive prostatic cancer
    Anita Mitra
    Translational Cancer Genetics Team, Section of Cancer Genetics, The Institute of Cancer Research, Sutton, Surrey, UK
    Histopathology 55:696-704. 2009
    To test the hypothesis that, in a matched series of prostatic cancers, either with or without BRCA1 or BRCA2 mutations, RAD51 protein expression is enhanced in association with BRCA mutation genotypes.
  26. ncbi CDK-dependent phosphorylation of BRCA2 as a regulatory mechanism for recombinational repair
    Fumiko Esashi
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 434:598-604. 2005
    ..show that the carboxy-terminal region of BRCA2, which interacts directly with the essential recombination protein RAD51, contains a site (serine 3291; S3291) that is phosphorylated by cyclin-dependent kinases...
  27. pmc A region of human BRCA2 containing multiple BRC repeats promotes RAD51-mediated strand exchange
    Mahmud K K Shivji
    Cancer Research UK Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, University of Cambridge, Hills Road, Cambridge CB2 2XZ, UK
    Nucleic Acids Res 34:4000-11. 2006
    Human BRCA2, a breast and ovarian cancer suppressor, binds to the DNA recombinase RAD51 through eight conserved BRC repeats, motifs of approximately 30 residues, dispersed across a large region of the protein...
  28. ncbi Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation
    Tomohiko Sugiyama
    Section of Microbiology and Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 277:31663-72. 2002
    The Rad51 nucleoprotein filament mediates DNA strand exchange, a key step of homologous recombination...
  29. ncbi HIV-1 Tat increases cell survival in response to cisplatin by stimulating Rad51 gene expression
    Galina Chipitsyna
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, 1900 North 12th Street, 015 96, Philadelphia, PA 19122, USA
    Oncogene 23:2664-71. 2004
    ..Examination of several proteins involved in the control of DNA repair showed elevated levels of Rad51, a key regulator of homologous recombination in cells expressing Tat...
  30. ncbi Cooperativity between Rad51 and C/EBP family transcription factors modulates basal and Tat-induced activation of the HIV-1 LTR in astrocytes
    Galina Chipitsyna
    Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania 19122, USA
    J Cell Physiol 207:605-13. 2006
    ..Recently, we demonstrated that expression of Tat in astrocytes and other neural cells leads to upregulation of Rad51, a major component of DNA repair via homologous recombination...
  31. ncbi DNA repair polymorphisms might contribute differentially on familial and sporadic breast cancer susceptibility: a study on a Portuguese population
    Sandra Costa
    ICVS, Life and Health Sciences Research Institute, Health Science School, Minho University, Braga, 4710 057, Portugal
    Breast Cancer Res Treat 103:209-17. 2007
    ..analysed DNA samples from 285 breast cancer patients and 442 control subjects, for XRCC1 Arg399Gln, XPD Lys751Gln, RAD51 G135C and XRCC3 Thr241Met polymorphisms using PCR-RFLP...
  32. ncbi Evaluation of genetic variation in the double-strand break repair pathway and bladder cancer risk
    Jonine D Figueroa
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Bethesda, MD, USA
    Carcinogenesis 28:1788-93. 2007
    ..BRIP1 and ZNF350), non-homologous end-joining (NHEJ) DNA repair (XRCC4) and homologous recombination (HR) repair (RAD51, XRCC2 and XRCC3). SNPs for RAD51 and XRCC2 covered most of the common variation...
  33. pmc DNA repair polymorphisms and outcome of chemotherapy for acute myelogenous leukemia: a report from the Children's Oncology Group
    D Bhatla
    Division of Hematology Oncology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Leukemia 22:265-72. 2008
    Polymorphisms of DNA repair genes RAD51 and XRCC3 increase susceptibility to acute myeloid leukemia (AML) in adults, an effect enhanced by deletion of the glutathione-S-transferase M1 (GSTM1) gene...
  34. doi Single-nucleotide polymorphisms in DNA double-strand break repair genes: association with head and neck cancer and interaction with tobacco use and alcohol consumption
    Joke Werbrouck
    Department of Anatomy, Embryology, Histology and Medical Physics, Ghent University, and Department of Radiation Oncology, Ghent University Hospital, Proeftuinstraat 86, B 9000 Gent, Belgium
    Mutat Res 656:74-81. 2008
    ..1843 A>G, XRCC3 c.562-14 A>G, XRCC3 c.722 C>T, Rad51 c.-3429 G>C, Rad51 c.-3392 G>T) and 4 SNPs in the non- homologous end joining DNA repair pathway (Lig4 c...
  35. doi Association between DNA damage, DNA repair genes variability and clinical characteristics in breast cancer patients
    Ewelina Synowiec
    Department of Molecular Genetics, University of Lodz, Lodz, Poland
    Mutat Res 648:65-72. 2008
    ..of basal (total endogenous) and endogenous oxidative DNA damage as well as polymorphism of the DNA repair genes: RAD51 (135 G/C), XRCC3 (Thr241Met), OGG1 (Ser326Cys) and XPD (Lys751Gln) in peripheral blood lymphocytes of 41 breast ..
  36. pmc Rad51 inhibition is an effective means of targeting DNA repair in glioma models and CD133+ tumor-derived cells
    Susan C Short
    UCL Cancer Institute, University College London, London, UK
    Neuro Oncol 13:487-99. 2011
    High grade gliomas (HGGs) are characterized by resistance to radiotherapy and chemotherapy. Targeting Rad51-dependent homologous recombination repair may be an effective target for chemo- and radiosensitization...
  37. doi Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51
    Kazuhiko Ochiai
    Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan
    FEBS Lett 585:1771-7. 2011
    ..BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks...
  38. doi Elevated expression of Rad51 is correlated with decreased survival in resectable esophageal squamous cell carcinoma
    Yong Li
    Department of Thoracic Surgery, Sun Yat sen University Cancer Center, Guangzhou City, Guangdong Province, PR China
    J Surg Oncol 104:617-22. 2011
    b>Rad51 plays a critical role in homologous recombination and correlates with many human malignancies. However, its role and clinical significance in esophageal squamous cell carcinoma (ESCC) has not been clarified...
  39. pmc Stimulation of the human RAD51 nucleofilament restricts HIV-1 integration in vitro and in infected cells
    O Cosnefroy
    Laboratoire MFP, UMR 5234, CNRS Université Victor Segalen Bordeaux 2, IFR 66 Pathologies Infectieuses et Cancers, Bordeaux, France
    J Virol 86:513-26. 2012
    ..The human RAD51 (hRAD51) protein plays a major role in homologous recombination (HR) DNA repair and was previously shown to interact with ..
  40. ncbi 135G>C and 172G>T polymorphism in the 5' untranslated region of RAD51 and sporadic endometrial cancer risk in Polish women
    Beata Smolarz
    Department of Pathology, Institute of Polish Mother s Memorial Hospital, Łódź, Poland
    Pol J Pathol 62:157-62. 2011
    ..The genetic variability in RAD51 may contribute to the appearance and progression of various cancers including EC.
  41. doi Role of the RAD51 G172T polymorphism in the clinical outcome of cervical cancer patients under concomitant chemoradiotherapy
    Augusto Nogueira
    Molecular Oncology GRP and Virology CI, Portuguese Institute of Oncology, Rua Dr Ant Bernardino Almeida, Porto, Portugal
    Gene 504:279-83. 2012
    ..The RAD51 protein is required for meiotic and mitotic recombination and plays a central role in homology-dependent ..
  42. doi Lack of association between the 135G/C RAD51 gene polymorphism and the risk of colorectal cancer among Polish population
    Bartosz Mucha
    Department of Clinical Chemistry and Biochemistry, Medical University in Łodz
    Pol Przegl Chir 84:358-62. 2012
    One of the major causes of carcinogenesis is loss of genome stability. RAD51 in process of homologous recombination (HR) played crucial role in maintenance integrity of genome through initiate of DNA double strand breaks repair...
  43. ncbi RAD51 gene polymorphisms and sporadic colorectal cancer risk in Poland
    Hanna Romanowicz-Makowska
    Department of Pathology, Institute of Polish Mother s Memorial Hospital, Lodz, Poland
    Pol J Pathol 63:193-8. 2012
    ..The genetic variability in RAD51 may contribute to the appearance and progression of various cancers including CRC...
  44. pmc A novel nucleic acid-binding protein that interacts with human rad51 recombinase
    O V Kovalenko
    Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA
    Nucleic Acids Res 25:4946-53. 1997
    ..system, we isolated a cDNA encoding a novel human protein, named Pir51, that strongly interacts with human Rad51 recombinase. Analysis in vitro confirmed the interaction between Rad51 and Pir51...
  45. ncbi Mutational analyses of the human Rad51-Tyr315 residue, a site for phosphorylation in leukaemia cells
    Yoshimasa Takizawa
    Graduate School of Integrated Science, Yokohama City University, 1 7 29 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Genes Cells 9:781-90. 2004
    The human Rad51 protein, which plays a central role in homologous recombination, catalyses homologous pairing...
  46. ncbi MDC1 interacts with Rad51 and facilitates homologous recombination
    Junran Zhang
    Department of Radiation Oncology, MGH Cancer Center, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Struct Mol Biol 12:902-9. 2005
    ..MDC1 forms a complex with Rad51 through a direct interaction with the forkhead-associated domain of MDC1, not the BRCA1 C-terminal domain...
  47. pmc Structure of human Rad51 protein filament from molecular modeling and site-specific linear dichroism spectroscopy
    Anna Reymer
    Physical Chemistry, Department of Chemical and Biological Engineering, Chalmers University of Technology, SE 41296 Goteborg, Sweden
    Proc Natl Acad Sci U S A 106:13248-53. 2009
    ..into the DNA strand-exchange reaction of homologous recombination, we solved a filament structure of a human Rad51 protein, combining molecular modeling with experimental data...
  48. doi RAD51 135G>C does not modify breast cancer risk in non-BRCA1/2 mutation carriers: evidence from a meta-analysis of 12 studies
    Ke Da Yu
    Department of Breast Surgery, Cancer Center Cancer Institute, Fudan University, 399 Ling Ling Road, Shanghai, 200032, People s Republic of China
    Breast Cancer Res Treat 126:365-71. 2011
    A single-nucleotide polymorphism (SNP) in the 5'-untranslated region (UTR) of RAD51, 135G>C (rs1801320), was reported to be associated with an increased risk of breast cancer among BRCA2 as well as BRCA1 carriers...
  49. pmc Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2
    Eloïse Dray
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut, USA
    Nat Struct Mol Biol 17:1255-9. 2010
    Homologous recombination mediated by RAD51 recombinase helps eliminate chromosomal lesions, such as DNA double-strand breaks induced by radiation or arising from injured DNA replication forks...
  50. ncbi M phase-specific phosphorylation of BRCA2 by Polo-like kinase 1 correlates with the dissociation of the BRCA2-P/CAF complex
    Horng Ru Lin
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245, USA
    J Biol Chem 278:35979-87. 2003
    ..that BRCA2 has a critical role in homologous recombination of DNA double-stranded breaks by interacting with RAD51. In addition, BRCA2 may have chromatin modifying activity through interaction with a histone acetyltransferase ..
  51. pmc FANCJ uses its motor ATPase to destabilize protein-DNA complexes, unwind triplexes, and inhibit RAD51 strand exchange
    Joshua A Sommers
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, National Institutes of Health Biomedical Research Center, Baltimore, Maryland 21224, USA
    J Biol Chem 284:7505-17. 2009
    ..Consistent with this, we show that FANCJ can inhibit RAD51 strand exchange, an activity that is likely to be important for its role in controlling DNA repair through ..
  52. ncbi Biochemical characterization of the human RAD51 protein. I. ATP hydrolysis
    Gregory Tombline
    Genetics and Molecular Biology Program, Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 277:14417-25. 2002
    ..A human RecA homolog, human RAD51 protein (hRAD51), facilitates eukaryotic recombination/repair, although its ability to hydrolyze ATP and/or promote strand ..
  53. ncbi Genetic polymorphisms of selected DNA repair genes, estrogen and progesterone receptor status, and breast cancer risk
    Kyoung Mu Lee
    Department of Preventive Medicine, Seoul National University College of Medicine, Chongno Gu, Seoul, Korea
    Clin Cancer Res 11:4620-6. 2005
    ..To evaluate the role of genetic polymorphisms of DNA repair genes in breast cancer, we conducted a hospital-based case-control study of Korean women...
  54. pmc Rad51 overexpression rescues radiation resistance in BRCA2-defective cancer cells
    Erika T Brown
    Department of Pathology, University of Colorado Health Sciences Center, RC 1 South Tower, 12801 East 17th Avenue, Aurora, Colorado 80010 7163, USA
    Mol Carcinog 48:105-9. 2009
    ..BRCA2 interacts with Rad51 in a complex manner involving internal BRC and C-terminal TR2 domains which play a key role in homologous ..
  55. pmc Single-molecule imaging brings Rad51 nucleoprotein filaments into focus
    Anthony L Forget
    Departments of Microbiology, and of Molecular and Cellular Biology, University of California, Davis, CA 95616 8665, USA
    Trends Cell Biol 20:269-76. 2010
    The Rad51 protein is essential for DNA repair by homologous recombination. After DNA damage, Rad51 localizes to nuclear foci that represent sites of DNA repair in vivo...
  56. pmc DNA damage and repair in endometrial cancer in correlation with the hOGG1 and RAD51 genes polymorphism
    Renata Krupa
    Laboratory of DNA Repair, Department of Molecular Genetics, University of Lodz, Banacha 12 16, 90 237, Lodz, Poland
    Mol Biol Rep 38:1163-70. 2011
    ..The hOGG1 gene encodes a glycosylase of base excision repair and RAD51 specifies a key protein in homologues recombination repair...
  57. pmc Bloom syndrome helicase stimulates RAD51 DNA strand exchange activity through a novel mechanism
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 284:26349-59. 2009
    ..Previously, we demonstrated that BLM can disrupt the RAD51-single-stranded DNA filament that promotes the initial steps of homologous recombination...
  58. pmc Real-time measurements of the nucleation, growth and dissociation of single Rad51-DNA nucleoprotein filaments
    Judith Mine
    Laboratoire Physico Chimie Curie, UMR CNRS 168, Institut Curie, Paris, France
    Nucleic Acids Res 35:7171-87. 2007
    Human Rad51 (hRad51), the protein central to DNA pairing and strand exchange during homologous recombination, polymerizes on DNA to form nucleoprotein filaments...
  59. pmc SUMO modification regulates BLM and RAD51 interaction at damaged replication forks
    Karen J Ouyang
    Committee on Genetics, Genomics, and Systems Biology, University of Chicago, Chicago, Illinois, USA
    PLoS Biol 7:e1000252. 2009
    ..At damaged forks, BLM interacts with RAD51 recombinase, the essential enzyme in HR that catalyzes homology-dependent strand invasion...
  60. ncbi Analysis of the G/C polymorphism in the 5'-untranslated region of the RAD51 gene in breast cancer
    Janusz Blasiak
    Department of Molecular Genetics, University of Lodz, Lodz, Poland
    Acta Biochim Pol 50:249-53. 2003
    The breast cancer suppressor proteins BRCA1 and BRCA2 interact with RAD51, a protein essential for maintaining genomic stability by playing a central role in homology-dependent recombinational repair of the DNA double-strand breaks...
  61. pmc Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA
    F E Benson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 13:5764-71. 1994
    ..coli recA (and its yeast homologue RAD51) has been cloned from a testis cDNA library, and its 37 kDa product (hRad51) purified to homogeneity...
  62. ncbi Role of BRCA2 in control of the RAD51 recombination and DNA repair protein
    A A Davies
    Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire EN6 3LD, South Mimms, United Kingdom
    Mol Cell 7:273-82. 2001
    ..Here, we show that BRCA2 plays a dual role in regulating the actions of RAD51, a protein essential for homologous recombination and DNA repair...
  63. ncbi P53 inhibits strand exchange and replication fork regression promoted by human Rad51
    Dennis Yoon
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    J Mol Biol 336:639-54. 2004
    ..explore the effects of p53 on strand exchange as well as regression of stalled replication forks promoted by human Rad51. We have found that p53 specifically inhibits strand exchange mediated by human Rad51, but not by Escherichia coli ..
  64. ncbi Genotype-phenotype relationship between DNA repair gene genetic polymorphisms and DNA repair capacity
    Aesun Shin
    Division of Cancer Prevention, National Cancer Control Research Institute, National Cancer Center, Goyang si, Gyeonggi Do, Korea
    Asian Pac J Cancer Prev 9:501-5. 2008
    ..DNA repair genes included were those involved in double-strand break repair (ATM, XRCC2, XRCC4, XRCC6, LIG4, RAD51, RAD52), base excision repair (LIG1), nucleotide excision repair (ERCC1), and mismatch repair (hMLH1)...
  65. pmc PALB2 functionally connects the breast cancer susceptibility proteins BRCA1 and BRCA2
    Fan Zhang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Mol Cancer Res 7:1110-8. 2009
    ..physically and functionally connects BRCA1 and BRCA2 into a DNA damage response network that also includes the RAD51 recombinase. PALB2 directly binds BRCA1, as determined with bacterially expressed fragments of each protein...
  66. pmc Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity
    Dmitry V Bugreev
    Department of Biochemistry, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    Proc Natl Acad Sci U S A 101:9988-93. 2004
    Human Rad51 (hRad51) protein plays a key role in homologous recombination and DNA repair...
  67. ncbi Analysis of RAD51 polymorphism and BRCA1 mutations in Polish women with breast cancer
    H Romanowicz-Makowska
    Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish Mother s Memorial Hospital, Lodz, Poland
    Exp Oncol 28:156-9. 2006
    Breast cancer is one of the major killers worldwide. The objectives of this study were to determine the frequency of BRCA1 germ-line mutations and the RAD51 G/C polymorphism in patients with breast cancer.
  68. pmc Interplay of Rad51 with NF-κB pathway stimulates expression of HIV-1
    Rafal Kaminski
    Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 9:e98304. 2014
    ..Here we demonstrate that the interplay between Rad51, a key regulator of the homologous recombination pathway of DNA repair and whose level is induced upon HIV-1 ..
  69. ncbi Expression of the human RAD51 gene during the cell cycle in primary human peripheral blood lymphocytes
    J Flygare
    Department of Bioscience at Novum, Karolinska Institutet, Huddinge, Sweden
    Biochim Biophys Acta 1312:231-6. 1996
    ..We have studied the expression of the human RAD51 (HsRAD51) gene in primary human peripheral blood lymphocytes (PBLs)...
  70. ncbi Biochemical characterization of the human RAD51 protein. III. Modulation of DNA binding by adenosine nucleotides
    Gregory Tombline
    Genetics and Molecular Biology Program, Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 277:14434-42. 2002
    ..and ATP/ATPgammaS affect the DNA binding and aggregation properties of the human RecA homolog human RAD51 protein (hRAD51). These studies have revealed significant differences between hRAD51 and RecA...
  71. ncbi Altered DNA binding by the human Rad51-R150Q mutant found in breast cancer patients
    Takako Ishida
    Laboratory of Structural Biology, Graduate School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biol Pharm Bull 30:1374-8. 2007
    The human Rad51 protein (HsRad51) catalyzes homologous pairing and strand exchange between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) during recombinational repair of double-stranded DNA breaks...
  72. ncbi Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen
    H Maacke
    Present address Clinic for Internal Medicine II, Friedrich Schiller University Jena, Erlanger Allee 101, 07743 Jena, Germany
    J Cancer Res Clin Oncol 128:219-22. 2002
    The recombination factor Rad51 is highly expressed in human pancreatic adenocarcinoma...
  73. doi Knockdown of Rad51 expression induces radiation- and chemo-sensitivity in osteosarcoma cells
    Li Qing Du
    Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union, Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin, 300192, China
    Med Oncol 28:1481-7. 2011
    ..b>Rad51 is the key protein of DNA homologous recombination repair...
  74. doi Lack of association of RAD51 genetic variations with hepatitis B virus clearance and occurrence of hepatocellular carcinoma in a Korean population
    Charisse Flerida A Pasaje
    Department of Life Science, Sogang University, Seoul, Republic of Korea
    J Med Virol 83:1892-9. 2011
    The RecA homolog, E. coli (S. cerevisiae) (RAD51) may modulate hepatitis B virus (HBV) infection by maintaining genome integrity and mediating homologous DNA repairs...
  75. ncbi Association of BRCA1 with Rad51 in mitotic and meiotic cells
    R Scully
    The Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 88:265-75. 1997
    BRCA1 immunostaining reveals discrete, nuclear foci during S phase of the cell cycle. Human Rad51, a homolog of bacterial RecA, behaves similarly. The two proteins were found to colocalize in vivo and to coimmunoprecipitate...
  76. pmc Fission yeast rad51 and dmc1, two efficient DNA recombinases forming helical nucleoprotein filaments
    Synthia Sauvageau
    Genome Stability Laboratory, Laval University Cancer Research Center, Hotel Dieu de Quebec, 9 McMahon, Quebec City, Quebec G1R 2J6, Canada
    Mol Cell Biol 25:4377-87. 2005
    ..Eukaryotic cells require two homologs of Escherichia coli RecA protein, Rad51 and Dmc1, for meiotic recombination...
  77. pmc Long-distance lateral diffusion of human Rad51 on double-stranded DNA
    Annette Graneli
    Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, 650 West 168th Street, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 103:1221-6. 2006
    b>Rad51 is the primary eukaryotic recombinase responsible for initiating DNA strand exchange during homologous recombination...
  78. ncbi Germline BRCA1 mutations and G/C polymorphism in the 5'-untranslated region of the RAD51 gene in Polish women with breast cancer
    Hanna Romanowicz-Makowska
    Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish Mother s Memorial Hospital, Lodz
    Pol J Pathol 56:161-5. 2005
    ..In the present work the distribution of genotypes and frequency of alleles of the RAD51 G/C polymorphism and the frequency of BRCA1 germ-line mutations in patients with breast cancer were investigated...
  79. doi Do BRCA1 modifiers also affect the risk of breast cancer in non-carriers?
    Anna Jakubowska
    International Hereditary Cancer Centre IHCC, Department of Genetics and Pathology, Pomeranian Medical University, Szczecin, Poland
    Eur J Cancer 45:837-42. 2009
    ..We genotyped seven SNPs in six distinct genes (PHB, RAD51, ITGB3, TGFB1, VEGF, MTHFR) in 1100 unselected Polish breast cancer patients and 1100 controls...
  80. ncbi [Association of polymorphisms of N372H in BRCA2 gene and 135G/C in RAD51 gene and breast cancers]
    Rui Hu
    Department of Mastologic Surgery, Sichuan Cancer Hospital, Sichuan Province, Chengdu 610041, China
    Sichuan Da Xue Xue Bao Yi Xue Ban 39:973-5. 2008
    To determine the associations between the polymorphisms of N372H in BRCA2 gene and 135G/C in RAD51 gene and breast cancers.
  81. doi RAD51 135G>C polymorphism and breast cancer risk: a meta-analysis
    Guo Wu Zhou
    Department of Respiration, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People s Republic of China
    Breast Cancer Res Treat 125:529-35. 2011
    Mutations in RAD51 gene are believed to be associated with elevated breast cancer risk. However, several case-control studies focusing on the association between RAD51 135G>C and breast cancer risk failed to achieve consensus...
  82. ncbi A single nucleotide polymorphism in the 5' untranslated region of RAD51 and ovarian cancer risk in Polish women
    H Romanowicz-Makowska
    Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish Mother s Memorial Hospital, Lodz, Poland
    Eur J Gynaecol Oncol 33:406-10. 2012
    DNA repair gene polymorphisms are known to influence cancer risk. The RAD51 gene encodes proteins essential for maintaining genomic stability by playing a central role in holmology-dependent recombinational repair of the DNA double-..
  83. pmc Differential contributions of mammalian Rad54 paralogs to recombination, DNA damage repair, and meiosis
    Joanna Wesoly
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Mol Cell Biol 26:976-89. 2006
    Homologous recombination is a versatile DNA damage repair pathway requiring Rad51 and Rad54...
  84. doi RAD51 135G>C polymorphism contributes to breast cancer susceptibility: a meta-analysis involving 26,444 subjects
    Zhanwei Wang
    Department of Epidemiology and Biostatistics, Nanjing Medical University, Nanjing, China
    Breast Cancer Res Treat 124:765-9. 2010
    b>RAD51 plays a key role in homologous recombination repair of double-stranded DNA breaks which may cause chromosomal breaks and genomic instability...
  85. ncbi A specific RAD51 haplotype increases breast cancer risk in Jewish non-Ashkenazi high-risk women
    Inabr Gal
    The Susanne Levy Gertner Oncogenetics Unit, The Danek Gertner Institute of Human Genetics, Chaim Sheba Medical Center, Tel Hashomer 52621, Israel
    Eur J Cancer 42:1129-34. 2006
    ..in determining familial breast cancer risk in addition to BRCA1/2 are mostly unknown, one strong candidate is RAD51. Jewish non-Ashkenazi women at high-risk for breast/ovarian cancer and ethnically matched controls were genotyped ..
  86. ncbi Polymorphisms of the BRCA2 and RAD51 genes in breast cancer
    Tomasz Sliwinski
    Department of Molecular Genetics, University of Lodz, Lodz, Poland
    Breast Cancer Res Treat 94:105-9. 2005
    ..a case-control study (150 cases and 150 controls) to test the association between three polymorphisms in BRCA2 and RAD51 genes and breast cancer risk. Genotypes were determined in DNA from blood cells by PCR-RFLP...
  87. pmc Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51
    S Desfarges
    UMR 5097 CNRS, Bordeaux, France
    Nucleic Acids Res 34:6215-24. 2006
    ..We demonstrated physical interactions between HIV-1 IN and RAD51 protein and showed that HIV-1 integrase activity could be inhibited both in the cell and in vitro by RAD51 protein...
  88. pmc The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment
    P L Chen
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Proc Natl Acad Sci U S A 95:5287-92. 1998
    ..Furthermore, direct binding of human RAD51 to each of the four single 30-amino acid BRC repeats located at the 5' portion of exon 11 of BRCA2 was ..
  89. ncbi RAD51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca2
    A K Wong
    Myriad Genetics, Inc, Salt Lake City, Utah 84108, USA
    J Biol Chem 272:31941-4. 1997
    Recent work has shown that the murine BRCA2 tumor suppressor protein interacts with the murine RAD51 protein. This interaction suggests that BRCA2 participates in DNA repair...
  90. ncbi Dynamic control of Rad51 recombinase by self-association and interaction with BRCA2
    David S Yu
    CR UK Department of Oncology and the Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
    Mol Cell 12:1029-41. 2003
    Here, we visualize GFP-Rad51 fusion proteins in the nucleus of living cells to demonstrate the dynamic compartmentalization of Rad51 by self-association or by binding to BRCA2...
  91. pmc RAD51 localization and activation following DNA damage
    Madalena Tarsounas
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, UK
    Philos Trans R Soc Lond B Biol Sci 359:87-93. 2004
    ..In response to ionizing radiation and other DNA-damaging agents, the RAD51 protein, which is essential for homologous recombination, relocalizes within the nucleus to form distinct foci ..
  92. ncbi hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51
    Kang Sup Shim
    Genetics and Molecular Biology Program, Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 279:30385-94. 2004
    The assembly of bacterial RecA, and its human homolog hRAD51, into an operational ADP/ATP-regulated DNA-protein (nucleoprotein) filament is essential for homologous recombination repair (HRR)...
  93. pmc A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments
    Sean D Sheridan
    Committee on Genetics, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 36:4057-66. 2008
    The eukaryotic RecA homologs Rad51 and Dmc1 are essential for strand exchange between homologous chromosomes during meiosis...
  94. doi Structural analysis of the human Rad51 protein-DNA complex filament by tryptophan fluorescence scanning analysis: transmission of allosteric effects between ATP binding and DNA binding
    Axelle Renodon-Corniere
    UMR 6204, Centre National de la Recherche Scientifique and Universite de Nantes, 44322 Nantes Cedex 3, France
    J Mol Biol 383:575-87. 2008
    Human Rad51 (HsRad51) catalyzes the strand exchange reaction, a crucial step in homologous recombination, by forming a filamentous complex with DNA...
  95. doi Emodin enhances gefitinib-induced cytotoxicity via Rad51 downregulation and ERK1/2 inactivation
    Ruey Shyang Chen
    Molecular Genetics of Microorganisms Laboratory, Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan
    Exp Cell Res 315:2658-72. 2009
    ..The recombinase protein Rad51 is essential for homologous recombination repair, and Rad51 overexpression is resistant to DNA double-strand break-..
  96. doi Variants in DNA double-strand break repair genes and risk of familial breast cancer in a South American population
    Lilian Jara
    Human Genetics Program, Institute of Biomedical Sciences ICBM, School of Medicine, University of Chile, Av Independencia 1027, P O Box 70061, Santiago, Chile
    Breast Cancer Res Treat 122:813-22. 2010
    The double-strand break (DSB) DNA repair pathway has been implicated in breast cancer (BC). RAD51 and its paralogs XRCC3 and RAD51D play an important role in the repair of DSB through homologous recombination (HR)...
  97. pmc Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA
    Jie Liu
    Department of Microbiology, University of California Davis, Davis, CA, USA
    Nat Struct Mol Biol 17:1260-2. 2010
    ..BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology ..
  98. ncbi Rapid exchange of A:T base pairs is essential for recognition of DNA homology by human Rad51 recombination protein
    R C Gupta
    Department of Genetics, Yale University, New Haven, Connecticut 06510, USA
    Mol Cell 4:705-14. 1999
    Human Rad51 belongs to a ubiquitous family of proteins that enable a single strand to recognize homology in duplex DNA, and thereby to initiate genetic exchanges and DNA repair, but the mechanism of recognition remains unknown...
  99. ncbi Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA
    A Shinohara
    Department of Biology, Faculty of Science, Osaka University, Japan
    Nat Genet 4:239-43. 1993
    b>Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double-strand breaks of DNA...
  100. pmc Interaction of p53 with the human Rad51 protein
    S Buchhop
    Institut für Humangenetik Universität zu Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Nucleic Acids Res 25:3868-74. 1997
    ..We recently reported that p53 can directly bind in vivo to human Rad51 (hRad51) protein and in vitro to its bacterial homologue RecA...
  101. pmc Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA
    E I Golub
    Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA
    Nucleic Acids Res 26:5388-93. 1998
    ..and stimulates homologous pairing and DNA strand exchange promoted in vitro by human recombination protein HsRad51. Co-immunoprecipitation revealed that purified RPA interacts physically with HsRad51, as well as with HsDmc1, the ..

Research Grants67

  1. Michael M Cox; Fiscal Year: 2014
    ..The competing proposal is focused on the bacterial RecA protein and proteins that regulate or augment RecA function...
  2. Timothy M Lohman; Fiscal Year: 2016
    ..In this manner we can examine the effects of the different SSB binding modes on the ability of RecA protein and SIPs to gain access to SSB-coated ssDNA under identical solution conditions and thus isolate the ..
  3. UNDERSTANDING HOMOLOGY IN GENETIC RECOMBINATION
    Scott F Singleton; Fiscal Year: 2012
    ..Recently, the Escherichia coli RecA protein's activities in SOS induction and homologous recombination have revealed RecA as a crucial player in ..
  4. Recombination and fork progression in bacteriophage T4
    Stephen W White; Fiscal Year: 2013
    ..UvsY and UvsW mediate the core of the homologous recombination reaction and are related to the eukaryotic proteins Rad51, Rad52 and Rad54, respectively...
  5. The MRAD9 Radioresistance Gene
    Howard B Lieberman; Fiscal Year: 2013
    ..We found that Rad9 physically interacts with Rad51 and functions in homologous recombination repair, and others reported that Rad9 can bind and regulate the activity ..
  6. The Novel Role of Branch Migration Protein Rad54 in Maintaining Genome Stability
    MATTHEW JOHN ROSSI; Fiscal Year: 2011
    ..The goals of this proposal are to understand how Rad51 affects the function of Rad54 and BLM in homologous recombination and investigate the non-canonical helicase ..
  7. Patrick Sung; Fiscal Year: 2016
    ..RECQ5 physically interacts with the RAD51 recombinase and displaces RAD51 from DNA...
  8. MECHANISTIC STUDIES OF GENETIC RECOMBINATION
    STEPHEN KOWALCZYKOWSKI; Fiscal Year: 1999
    ..The focus of this proposal is on the Escherichia coli recA protein and on the contributions that other recombinationally important proteins such as SSB protein, recBCD enzyme,..
  9. DNA HELICASES IN RECOMBINATION AND REPAIR
    Hannah L Klein; Fiscal Year: 2010
    ..The Srs2 DNA helicase and the SWI/SNF-like proteins Rad54, Rdh54 and Tid4 act against Rad51, an essential factor in homologous recombination...
  10. Simon N Powell; Fiscal Year: 2016
    ..The goals of this second aim are to understand the Rad52-Rad51 pathway in mammalian cells as it may give insight on how tumor cells defective in BRCA1-BRCA2 pathway survive ..
  11. RECOMBINATION IN VITRO: ENZYMOLOGY AND INTERMEDIATES
    CHARLES RADDING; Fiscal Year: 1993
    The enzymology of homologous pairing and strand exchange promoted by E. coli recA protein's unique...
  12. Kamel Khalili; Fiscal Year: 2015
    ..DNA damage is well documented, recent observations (shown here) point to the ability of Tat in the induction of Rad51 expression in primary microglia and astrocytes, the two cell types that play an important role in ..
  13. Cellular functions of the essential PcrA helicase in Staphylococcus aureus
    Saleem A Khan; Fiscal Year: 2013
    ..conditional knockouts of pcrA are hyperrecombinogenic, suggesting an essential role for PcrA in the regulation of RecA-mediated DNA recombination. We have characterized the PcrA protein of S...
  14. Nuclear IRS-1-DNA repair and mutagenesis in medulloblastoma
    Krzysztof Reiss; Fiscal Year: 2012
    ..component of DNA repair (homologous recombination DNA repair, HRR) via a direct interaction between IRS-1 and Rad51 detected at the sites of damaged DNA...
  15. Gary M Kupfer; Fiscal Year: 2014
    ..FA pathway with other more defined pathways involved in cellular response to DNA damage, such as ATR, BRCA1, and RAD51. Recent work has served to shift focus of FA biology and the normal functions of these proteins onto homologous ..
  16. Marja T Nevalainen; Fiscal Year: 2014
    ..Inhibition of Stat5a/b increases radiatio-induced DNA- damage in PCa cells and 7) Stat5a/b up-regulates Rad51 which is the key protein in Homologous Recombination (HR)-DNA repair in mammalian cells...
  17. Roles of hMSH5 in DNA recombination and cellular response to anticancer treatmen
    Chengtao Her; Fiscal Year: 2013
    ..of endogenous hMSH5 and hMSH4 proteins, and the DSB- induced hMSH5 loading is dependent on functional hMRE11 and hRad51 proteins...
  18. Wolf Dietrich Heyer; Fiscal Year: 2016
    ..Specifically, we focus on the mechanism and regulation of the assembly of the Rad51-ssDNA filament, a key intermediate in recombinational repair...
  19. Wolf Dietrich Heyer; Fiscal Year: 2016
    ..The Rad54 motor protein plays a key role in HR, dissociating Rad51 from heteroduplex DNA to allow DNA polymerases access to the invading 3'end...
  20. Role of RPA Hyperphosphorylation in DNA Damage Responses
    MOISES ALEJANDRO SERRANO; Fiscal Year: 2012
    ..determine: 1) the role of hyperphosphorylated RPA in the suppression of origin firing;2) the interaction between Rad51/Rad52 and RPA and its effect by RPA hyperphosphorylatlon;and 3) the cellular molecular interactions stimulated and/..
  21. Understanding the role of RAD51C complexes in recombination and repair
    Yilun Liu; Fiscal Year: 2012
    ..Equally, we need to define the roles of the known DNA break repair proteins, such as the RAD51 paralogs, as mutations in these proteins have been associated with increased cancer risks and decreased survival ..
  22. Priscilla K Cooper; Fiscal Year: 2016
    ..is known to be important for maintaining genomic integrity during S-phase, and also interacts directly with the RAD51 recombinase...
  23. Edward H Egelman; Fiscal Year: 2016
    ..What emerges in our studies of proteins like bacterial RecA and human Rad51 is that the polymers they form are highly dynamic, and that these polymers cannot be reduced to a single structure...
  24. STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2016
    ..and function of RecBCD enzyme, and also to establish when and where the [unreadable]- activated enzyme binds RecA protein, and how it is loaded onto the [unreadable]-containing single-stranded DNA...
  25. DNA DAMAGE, REPAIR, AND MUTAGENESIS
    IRWIN TESSMAN; Fiscal Year: 1992
    ..The RecA protein of E...
  26. RECOMBINATION IN VITRO--ENZYMOLOGY AND INTERMEDIATES
    CHARLES RADDING; Fiscal Year: 2003
    E. Coli RecA protein forms a right-handed helical nucleoprotein filament on single-stranded DNA which catalyzes a search for homology, assimilates homologous duplex DNA, and effects the complete exchange of strands to produce a new ..
  27. RECA PROTEIN STRUCTURE AND FUNCTION
    KEVIN MCENTEE; Fiscal Year: 1993
    ..proposal are to elucidate and characterize fundamental interactions that are important for the activities of the RecA protein of Escherichia coli...
  28. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2004
    ..This proposal was funded to examine the mechanisms used by the Gc RecA protein to mediate recombination and repair in this human specific pathogen and to determine how gonococcal RecA ..
  29. TARGETING NATIVE DOUBLE HELIX STRUCTURES AND FUNCTIONS
    DAVID ZARLING; Fiscal Year: 1999
    ..The PI is developing native DNA targeting reactions mediated by E. coli RecA protein which catalyze the hybridization of pairs of complementary single-stranded (css) DNA probes to homologous ..
  30. RADIOBIOLOGY OF D RADIODURANS--GENETIC APPROACH
    Michael Daly; Fiscal Year: 2001
    ..Surprisingly, we detected two DSB- recombinational repair pathways, one that is recA-dependent and another that is recA-independent...
  31. MECHANISM OF INDUCTION OF VIRAL AND CELLULAR FUNCTION
    David Mount; Fiscal Year: 1993
    ..the interaction of LexA protein with such operators will be studied using methods previously established for the recA operator...
  32. GENETIC ANALYSIS OF THE RECA PROTEIN OF E. COLI
    George Weinstock; Fiscal Year: 1991
    ..The long-term goals of this project are to understand cellular responses to DNA damage. In bacteria, the RecA protein controls the synthesis of enzymes that repair DNA and also directly participates in several repair pathways...
  33. Structure/function of RecA protein from P. aeruginosa
    Michael Cox; Fiscal Year: 2006
    ..The RecA protein of E...
  34. Polymerization and Activation of Bacterial RecA Proteins
    FREDERICK WHIPPLE; Fiscal Year: 2004
    Bacterial genetic techniques will be used to investigate the mechanism by which molecules of the RecA protein of the bacterium Escherichia coli polymerize around DNA to form an active nucleoprotein complex...
  35. DNA RECOMBINASE REACTION MECHANISMS
    FLOYD BRYANT; Fiscal Year: 2002
    The goal of this research project is to determine how the recA protein of E. coli utilizes the energy of ATP hydrolysis to promote DNA strand pairing reactions during homologous genetic recombination and recombinational DNA repair...
  36. Repair of DNA breaks in humans: the role of Rad54 protein
    Alexander V Mazin; Fiscal Year: 2013
    ..Previously, it was shown that hRad54 stimulates DNA strand exchange activity of hRad51, a key protein of HR...
  37. FASEB SRC on Genetic Recombination and Genome Rearrangements
    JOHN HJ PETRINI; Fiscal Year: 2011
    ..the product of the BRCA2 gene, which is mutated in a subset of hereditary breast cancers, interacts directly with Rad51 and is essential for homologous recombination...
  38. Identification of inhibitors of RAD54, an important DNA repair protein
    Alexander V Mazin; Fiscal Year: 2013
    ..The specificity of th selected inhibitors will be examined using human RAD51 protein that is structurally unrelated to RAD54...
  39. Philip P Connell; Fiscal Year: 2016
    ..Our on-going research plan involves a multistep screen for identifying small molecule inhibitors of human RAD51, which is the central protein involved in HR...
  40. STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2015
    ..We will determine their DNA binding specificity, their capacity to stimulate DNA strand exchange promoted by hRAD51, and their ability to stabilize hRAD51 nucleoprotein filaments from disassembly both intrinsically and by ..
  41. Development of RAD52 Inhibitors to Induce Lethality of BRCA2-Deficient Cells
    Alexander V Mazin; Fiscal Year: 2013
    ..The specificity of the selected inhibitors will be examined using human RAD51 protein that is structurally unrelated to RAD52...
  42. Cellular and Biochemical Regulation of Human Rad51 Function
    Kendall L Knight; Fiscal Year: 2010
    The goal of our research is to provide a cellular and molecular understanding of human Rad51-mediated DNA repair...
  43. STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2016
    ..The proteins that will be examined include the DNA strand exchange proteins, RecA and Rad51;the mediators and modulators of RecA/Rad51 function;and the finally the nucleoprotein- and chromatin-remodeling ..
  44. The Roles of Trm9 and tRNA Methylation in the DNA Damage Response
    Thomas J Begley; Fiscal Year: 2010
    ..human Trm9 activity to the regulation of specific DNA repair proteins (Rad54, Brca2, Bccip, Ercc8, Tdg, Msh4, Rad51, and DNA Ligase IV), and we will study how Trm9 affects their damage-induced translation...
  45. Jack D Griffith; Fiscal Year: 2015
    ..In AIM II, experiments using transgenic mice will further probe role of TRF2, and work on the Rad51 paralogs and the WRN helicase will be conducted to examine the interaction of these repair factors with the ..
  46. Peter M Glazer; Fiscal Year: 2016
    ..of the DNA mismatch repair (MMR) factors, MLH1 and MSH2, and of the homology- dependent repair (HDR) factors, RAD51 and BRCA1. The MMR and HDR pathways are critical for maintaining genomic integrity...
  47. Nancy M Hollingsworth; Fiscal Year: 2016
    ..there are two recombinases required for interhomolog (IH) recombination, the meiosis-specific Dmc1 protein and Rad51, which is also used in vegetative cells...
  48. Lorraine S Symington; Fiscal Year: 2016
    ..The single-stranded DNA formed functions as a sensor for the DNA damage response and is the substrate for Rad51 catalyzed strand exchange between the ssDNA bound by Rad51 and homologous duplex DNA...
  49. Scott Keeney; Fiscal Year: 2016
    ..which ATM is activated in response to SPO11- generated DSBs will be determined, focusing on the role of the MRE11-RAD51-NBS1 complex...
  50. Douglas K Bishop; Fiscal Year: 2016
    ..There are two RecA-like recombinases in most eukaryotic organisms, including budding yeast and humans;Rad51 is the only RecA-like recombinase involved in mitotic recombination...
  51. DNA Recombinational Repair: Role of the p53 Tumor Suppressor Protein
    Simon N Powell; Fiscal Year: 2010
    ..We determined that p53 was not acting directly via the Rad51 recombinase, but through the Replication Protein A (RPA)...
  52. Susan S Wallace; Fiscal Year: 2014
    ..repair enzymes the HhH-GPD superfamily of DNA glycosylases, the Fpg/Nei family of DNA glycosylases and the RecA-RAD51 family of recombinases, recognize and process their substrates and how germ line and tumor associated variants of ..
  53. Neil Hunter; Fiscal Year: 2016
    ..We will also test the idea that Srs2 is prevented from disrupting filaments of RecA proteins, Rad51 and Dmc1, during meiosis by the so-called "mediator" proteins...
  54. Claudia Wiese; Fiscal Year: 2016
    ..1, a region that is commonly gained in breast cancer. Based on sequence homology to RAD51 Associated Protein 1 (RAD51AP1), a critical factor in homologous recombination repair (HRR), we have tested and ..
  55. William K Holloman; Fiscal Year: 2015
    ..Primary components of this pathway in eukaryotes include Rad51 whose function is to search for DNA sequence homology and promote strand exchange, plus factors that enable, ..
  56. BRCA2 model system
    William K Holloman; Fiscal Year: 2013
    ..Primary components of this pathway in eukaryotes include Rad51 whose function is to search for DNA sequence homology and promote strand exchange, plus factors that enable, ..
  57. Zhong Yun; Fiscal Year: 2014
    ..including the DNA mismatch repair (MMR) factors, MLH1 and MSH2, and the homology-dependent repair (HDR) factors, RAD51 and BRCA1...
  58. DNA Repair in Cancer Biology and Therapy
    Peter M Glazer; Fiscal Year: 2012
    ..will lead a project that focuses on the transcriptional regulation of the homology-dependent repair (HDR) genes, RAD51 and BRCA1, in hypoxic cancer cells...
  59. Douglas E Koshland; Fiscal Year: 2015
    ..Remarkably, the formation of these hybrids is mediated by the RAD51 repair pathway...
  60. Richard Fishel; Fiscal Year: 2015
    ..Determine the kinetics and mechanism of the RAD51 homology search, strand exchange and remodeling function(s) within chromatin, and 2...
  61. Single Molecule Studies of Recombination and Chromosome Pairing in Meiosis
    Richard Fishel; Fiscal Year: 2013
    ..DSB repair requires the RAD51 recombinase to identify homology and perform strand exchange between homologous chromosomes that results in a D-..
  62. Plasmodium Recombination of Machinery
    Nirbhay Kumar; Fiscal Year: 2009
    ..In other eukaryotes, homologous recombination (HR) plays a major role in chromosomal rearrangements, and Rad51 and Dmc1 proteins, the eukaryotic counterparts of bacterial RecA recombinase, are central molecules involved in HR ..
  63. Use of RecA to Promote Gene Targeting
    Jeffrey J Essner; Fiscal Year: 2010
    ..Our method uses a significantly modified version of the bacterial recombinase protein RecA that has greatly enhanced activities...
  64. Structural Studies of RecA-DNA Complexes
    Charles Bell; Fiscal Year: 2009
    ..E. coil RecA is 30% identical in amino acid sequence to the human enzyme (Rad51) and therefore will have a closely related biochemical mechanism...
  65. HTS for Protein Splicing Inhibitors as TB Drugs
    Henry Paulus; Fiscal Year: 2003
    ..tuberculosis DnaB and RecA proteins by protein splicing...
  66. Mechanisms of Carcinogenesis in BRCA2 Mutant Cells
    Jeffrey Holt; Fiscal Year: 2005
    ..Our preliminary data in mouse BRCA2-- cells shows that Rad51 is rapidly cleaved by caspase-3 in BRCA2 defective cells and that overexpression of BRCA2 or Rad51, or inhibition ..
  67. Mechanistic Studies of BRCA2-RAD51 Mediated DNA Repair
    Erika Brown; Fiscal Year: 2006
    ..Her studies have shown that the DNA repair protein RAD51, which associates with the protein BRCA2, fails to move from the cytoplasm into the nucleus after induction of ..