RAD21

Summary

Gene Symbol: RAD21
Description: RAD21 cohesin complex component
Alias: CDLS4, HR21, HRAD21, MCD1, NXP1, SCC1, hHR21, double-strand-break repair protein rad21 homolog, NXP-1, RAD21 homolog, SCC1 homolog, kleisin, nuclear matrix protein 1, protein involved in DNA double-strand break repair, sister chromatid cohesion 1
Species: human

Top Publications

  1. ncbi Analysis of spontaneous and double-strand break-induced recombination in rad mutants of S. pombe
    E A Fortunato
    Department of Biology, University of California San Diego, La Jolla 92093 0322, USA
    Mutat Res 364:14-60. 1996
  2. pmc Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation
    Mari Shimura
    Department of Intractable Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
    J Cell Biol 194:721-35. 2011
  3. ncbi Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse
    M J McKay
    MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, 3000DR, The Netherlands
    Genomics 36:305-15. 1996
  4. pmc Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans
    S Parisi
    Institute of General Microbiology, University of Bern, 3012 Bern, Switzerland
    Mol Cell Biol 19:3515-28. 1999
  5. pmc Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes
    A Losada
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Cell Biol 150:405-16. 2000
  6. pmc Characterization of vertebrate cohesin complexes and their regulation in prophase
    I Sumara
    Research Institute of Molecular Pathology IMP, A 1030 Vienna, Austria
    J Cell Biol 151:749-62. 2000
  7. ncbi Cohesin cleavage by separase required for anaphase and cytokinesis in human cells
    S Hauf
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 293:1320-3. 2001
  8. ncbi Caspase proteolysis of the cohesin component RAD21 promotes apoptosis
    Feng Chen
    Robert H Lurie Comprehensive Cancer Center and the Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA
    J Biol Chem 277:16775-81. 2002
  9. ncbi A chromatin remodelling complex that loads cohesin onto human chromosomes
    Mohamed Ali Hakimi
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 418:994-8. 2002
  10. ncbi Cohesin defects lead to premature sister chromatid separation, kinetochore dysfunction, and spindle-assembly checkpoint activation
    Md Tozammel Hoque
    Laboratory of Cell Cycle Regulation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Japan
    J Biol Chem 277:42306-14. 2002

Research Grants

  1. Matthew J O'Connell; Fiscal Year: 2015
  2. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
  3. Paul M Lieberman; Fiscal Year: 2015
  4. The Cytogenetics of Meiosis of Maize
    WILLIAM ZACHEUS CANDE; Fiscal Year: 2010
  5. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2010
  6. Ann J Feeney; Fiscal Year: 2014
  7. Molecular Basis of Aneuploidy
    Debananda Pati; Fiscal Year: 2010
  8. Role of MRK in DNA Damage Response
    Rosamaria Ruggieri; Fiscal Year: 2007
  9. Antigen Presentation Pathway by CD1 Molecules
    Mitchell Kronenberg; Fiscal Year: 2006
  10. Mitotic Regulation of Apoptosis in Leukemia
    Debananda Pati; Fiscal Year: 2010

Detail Information

Publications213 found, 100 shown here

  1. ncbi Analysis of spontaneous and double-strand break-induced recombination in rad mutants of S. pombe
    E A Fortunato
    Department of Biology, University of California San Diego, La Jolla 92093 0322, USA
    Mutat Res 364:14-60. 1996
    ..cerevisiae RAD3) and rad10 (homologue of S. cerevisiae RAD1) involved in nucleotide excision repair; rad21 and rad22 (homologue of S. cerevisiae RAD52) involved in the repair of ionizing radiation-induced DNA damage...
  2. pmc Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation
    Mari Shimura
    Department of Intractable Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
    J Cell Biol 194:721-35. 2011
    ..In Vpr-expressing cells, hRad21, hSgo1, and hMis12, which are crucial for proper chromosome segregation, were displaced from the centromeres of ..
  3. ncbi Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse
    M J McKay
    MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, 3000DR, The Netherlands
    Genomics 36:305-15. 1996
    ..The mHR21(sp) (mouse homolog of Rad21, S. pombe) and hHR21(sp) (human homolog of Rad21, S. pombe) predicted proteins were 96% identical, whereas the human and S...
  4. pmc Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans
    S Parisi
    Institute of General Microbiology, University of Bern, 3012 Bern, Switzerland
    Mol Cell Biol 19:3515-28. 1999
    Our work and that of others defined mitosis-specific (Rad21 subfamily) and meiosis-specific (Rec8 subfamily) proteins involved in sister chromatid cohesion in several eukaryotes, including humans...
  5. pmc Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes
    A Losada
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Cell Biol 150:405-16. 2000
    ..These results shed light on the mechanism by which sister chromatid cohesion is partially dissolved in early mitosis, far before the onset of anaphase, in vertebrate cells...
  6. pmc Characterization of vertebrate cohesin complexes and their regulation in prophase
    I Sumara
    Research Institute of Molecular Pathology IMP, A 1030 Vienna, Austria
    J Cell Biol 151:749-62. 2000
    ..Both complexes contain SMC1, SMC3, SCC1, and either one of the yeast Scc3p orthologs SA1 and SA2. SA1 is also a subunit of 14S cohesin in Xenopus...
  7. ncbi Cohesin cleavage by separase required for anaphase and cytokinesis in human cells
    S Hauf
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 293:1320-3. 2001
    ..We have identified two separase cleavage sites in the human cohesin subunit SCC1 and have conditionally expressed noncleavable SCC1 mutants in human cells...
  8. ncbi Caspase proteolysis of the cohesin component RAD21 promotes apoptosis
    Feng Chen
    Robert H Lurie Comprehensive Cancer Center and the Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA
    J Biol Chem 277:16775-81. 2002
    ..Using an expression cloning strategy we recently developed, we isolated human RAD21/SCC1/MCD1 as a novel caspase substrate...
  9. ncbi A chromatin remodelling complex that loads cohesin onto human chromosomes
    Mohamed Ali Hakimi
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 418:994-8. 2002
    ..We show that the hRAD21 subunit of the cohesin complex directly interacts with the ATPase subunit SNF2h...
  10. ncbi Cohesin defects lead to premature sister chromatid separation, kinetochore dysfunction, and spindle-assembly checkpoint activation
    Md Tozammel Hoque
    Laboratory of Cell Cycle Regulation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Japan
    J Biol Chem 277:42306-14. 2002
    Scc1/Mcd1 is a component of the cohesin complex that plays an essential role in sister chromatid cohesion in eukaryote cells...
  11. pmc Linking sister chromatid cohesion and apoptosis: role of Rad21
    Debananda Pati
    Texas Children s Cancer Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:8267-77. 2002
    ..We show that cleavage of human Rad21 (hRad21) also occurs during apoptosis induced by diverse stimuli...
  12. pmc Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2
    Silke Hauf
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e69. 2005
    Cohesin is a protein complex that is required to hold sister chromatids together. Cleavage of the Scc1 subunit of cohesin by the protease separase releases the complex from chromosomes and thereby enables the separation of sister ..
  13. ncbi Suppression of RAD21 gene expression decreases cell growth and enhances cytotoxicity of etoposide and bleomycin in human breast cancer cells
    Josephine M Atienza
    Sequenom, Inc, 3595 John Hopkins Court, San Diego, CA 92121, USA
    Mol Cancer Ther 4:361-8. 2005
    ..case-control association study done in our laboratory has identified a single nucleotide polymorphism located in RAD21 as being significantly associated with breast cancer susceptibility...
  14. ncbi Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates
    Susannah Rankin
    Systems Biology Department, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 18:185-200. 2005
    ..We speculate that the protein, which we have named sororin, regulates the ability of the cohesin complex to mediate sister chromatid cohesion, perhaps by altering the nature of the interaction of cohesin with the chromosomes...
  15. ncbi Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts
    Ana Losada
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    J Cell Sci 118:2133-41. 2005
    ..Depletion of the cohesin subunit Scc1 by RNA interference leads to the assembly of chromosomes with severe cohesion defects...
  16. pmc Metazoan Scc4 homologs link sister chromatid cohesion to cell and axon migration guidance
    Vlad C Seitan
    Institute of Human Genetics, University of Newcastle, Newcastle upon Tyne, United Kingdom
    PLoS Biol 4:e242. 2006
    ....
  17. ncbi Wapl controls the dynamic association of cohesin with chromatin
    Stephanie Kueng
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Cell 127:955-67. 2006
    ..Wapl depletion also increases the residence time of cohesin on chromatin in interphase. Our data indicate that Wapl is required to unlock cohesin from a particular state in which it is stably bound to chromatin...
  18. ncbi Sororin is required for stable binding of cohesin to chromatin and for sister chromatid cohesion in interphase
    Julia Schmitz
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 17:630-6. 2007
    ..Our data indicate that sororin interacts with chromatin-bound cohesin and functions during the establishment or maintenance of cohesion in S or G2 phase, respectively...
  19. pmc Cohesins localize with CTCF at the KSHV latency control region and at cellular c-myc and H19/Igf2 insulators
    William Stedman
    Gene Regulation Program, The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    EMBO J 27:654-66. 2008
    ..siRNA depletion of RAD21 from latently infected cells caused an increase in K14 and ORF74, and lytic inducers caused a rapid dissociation ..
  20. pmc Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1
    Keishi Shintomi
    Chromosome Dynamics Laboratory, RIKEN Advanced Science Institute, Wako, Saitama 351 0198, Japan
    Genes Dev 23:2224-36. 2009
    ..Evidence is also presented that Sgo1 plays a hitherto underappreciated role in stabilizing cohesin along chromosome arms, which is antagonized by the mitotic kinases polo-like kinsase (Plk1) and aurora B...
  21. doi Sororin mediates sister chromatid cohesion by antagonizing Wapl
    Tomoko Nishiyama
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 143:737-49. 2010
    ..The mechanism we describe here may therefore be widely conserved among different species...
  22. pmc Enhanced RAD21 cohesin expression confers poor prognosis and resistance to chemotherapy in high grade luminal, basal and HER2 breast cancers
    Huiling Xu
    Research Division, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett Street, Melbourne, VIC 8006, Australia
    Breast Cancer Res 13:R9. 2011
    b>RAD21 is a component of the cohesin complex, which is essential for chromosome segregation and error-free DNA repair...
  23. pmc Interaction of Sororin protein with polo-like kinase 1 mediates resolution of chromosomal arm cohesion
    Nenggang Zhang
    Texas Children s Cancer and Hematology Center, Department of Pediatric Hematology Oncology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 286:41826-37. 2011
    ....
  24. pmc Enhanced RAD21 cohesin expression confers poor prognosis in BRCA2 and BRCAX, but not BRCA1 familial breast cancers
    Max Yan
    Department of Anatomical Pathology, Prince of Wales Hospital, Barker Street, Randwick, 2031, Australia
    Breast Cancer Res 14:R69. 2012
    The RAD21 gene encodes a key component of the cohesin complex, which is essential for chromosome segregation, and together with BRCA1 and BRCA2, for high-fidelity DNA repair by homologous recombination...
  25. ncbi Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres
    M T Hoque
    Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, 4259 Nagatsuta, Midori ku, Yokohama, 226 8501, Japan
    J Biol Chem 276:5059-67. 2001
    ..b>hRad21 is a nuclear phosphorylated protein...
  26. ncbi RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in prostate cancer
    Kati P Porkka
    Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland
    Genes Chromosomes Cancer 39:1-10. 2004
    ..Two genes from chromosomal region 8q24-RAD21 and KIAA0196-showed increased expression in clinical prostate carcinomas and were also amplified in 30-40% of ..
  27. pmc Cell cycle control of Kaposi's sarcoma-associated herpesvirus latency transcription by CTCF-cohesin interactions
    Hyojeung Kang
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Virol 83:6199-210. 2009
    ..by the cellular chromatin boundary factor CTCF and chromosome structural maintenance proteins SMC1, SMC3, and RAD21, which comprise the cohesin complex...
  28. pmc Calpain-1 cleaves Rad21 to promote sister chromatid separation
    Anil K Panigrahi
    Department of Pediatric Hematology Oncology, Baylor College of Medicine, 1102 Bates Avenue, Suite 1220, Houston, TX 77030
    Mol Cell Biol 31:4335-47. 2011
    ..Sister chromatids are held together by cohesin, a multiprotein ring-like complex comprised of Rad21, Smc1, Smc3, and SA2 (or SA1)...
  29. pmc Cohesins determine the attachment manner of kinetochores to spindle microtubules at meiosis I in fission yeast
    Shihori Yokobayashi
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Mol Cell Biol 23:3965-73. 2003
    ..A multisubunit complex called cohesin, including Rad21/Scc1, plays a crucial role in sister chromatid cohesion and equational segregation at mitosis...
  30. pmc Sequential loading of cohesin subunits during the first meiotic prophase of grasshoppers
    Ana M Valdeolmillos
    Departamento de Biologia, Edificio de Biológicas, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS Genet 3:e28. 2007
    ..its localization resembles the location of the unsynapsed axial elements, whereas radiation-sensitive mutant 21 (RAD21) (sister chromatid cohesion protein 1, SCC1) and stromal antigen protein 1 (SA1) (sister chromatid cohesion ..
  31. pmc Cohesin Is limiting for the suppression of DNA damage-induced recombination between homologous chromosomes
    Shay Covo
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences NIEHS, National Institutes of Health NIH, Research Triangle Park, North Carolina, United States of America
    PLoS Genet 6:e1001006. 2010
    ..Unlike RAD50 and RAD51, which play a direct role in HR, a 4-fold reduction in the number of essential MCD1 sister chromatid cohesion subunit genes affected survival of gamma-irradiated G(2)/M cells...
  32. pmc A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction
    P Pasierbek
    Department of Cytology and Genetics, Institute of Botany, University of Vienna, A 1030 Vienna, Austria
    Genes Dev 15:1349-60. 2001
    We have studied four Caenorhabditis elegans homologs of the Rad21/Scc1/Rec8 sister-chromatid cohesion protein family. Based on the RNAi phenotype and protein localization, it is concluded that one of them, W02A2...
  33. ncbi Changes in the expression and localization of cohesin subunits during meiosis in a non-mammalian vertebrate, the medaka fish
    Toshiharu Iwai
    Laboratory of Molecular and Cellular Interactions, Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    Gene Expr Patterns 4:495-504. 2004
    ..subunits during meiosis in non-mammalian species, we isolated cDNA clones encoding SMC1alpha, SMC1beta, SMC3 and Rad21 in the medaka and produced antibodies against recombinant proteins...
  34. ncbi Two putative acetyltransferases, san and deco, are required for establishing sister chromatid cohesion in Drosophila
    Byron C Williams
    Department of Molecular Biology and Genetics, 427 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Curr Biol 13:2025-36. 2003
    ..However, many aspects of the mechanisms that establish and maintain cohesion during mitosis remain unclear...
  35. pmc Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G1 phase
    K Furuya
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606, Japan
    Genes Dev 12:3408-18. 1998
    The loss of sister chromatid cohesion triggers anaphase spindle movement. The budding yeast Mcd1/Scc1 protein, called cohesin, is required for associating chromatids, and proteins homologous to it exist in a variety of eukaryotes...
  36. ncbi Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast
    Yusuke Toyoda
    Department of Gene Mechanisms, Graduate School of Biostudies, 606 8502, Kyoto, Japan
    Curr Biol 12:347-58. 2002
    ..The failure to form cohesion in the S phase results in premature separation of chromatids in G2/M. Mitotic kinetochores free from microtubules or the lack of tension are known to activate spindle checkpoint...
  37. ncbi Studies on substrate recognition by the budding yeast separase
    Matt Sullivan
    Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 279:1191-6. 2004
    Sister chromatid cohesion is resolved at anaphase onset when separase, a site-specific protease, cleaves the Scc1 subunit of the chromosomal cohesin complex that is responsible for holding sister chromatids together...
  38. ncbi Cdc48 is required for the stability of Cut1/separase in mitotic anaphase
    Nobuyasu Ikai
    Department of Gene Mechanism, Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    J Struct Biol 156:50-61. 2006
    Separase, a large protease essential for sister chromatid separation, cleaves the cohesin subunit Scc1/Rad21 during anaphase and leads to dissociation of the link between sister chromatids...
  39. pmc Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved
    Jenny M Rhodes
    Department of Pathology, Dunedin School of Medicine, The University of Otago, Dunedin, New Zealand
    Dev Biol 344:637-49. 2010
    ..We analyzed zebrafish embryos null for cohesin subunit rad21 using microarrays to determine global effects of cohesin on gene expression during embryogenesis...
  40. ncbi Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion
    Dmitri Ivanov
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Curr Biol 12:323-8. 2002
    ..In Saccharomyces cerevisiae, a complex consisting of Scc1, Smc1, Smc3, and Scc3 proteins, called "cohesin," mediates the connection between sister chromatids...
  41. ncbi Localization of human SMC1 protein at kinetochores
    Heather C Gregson
    University of California, Irvine, College of Medicine, Department of Biological Chemistry, 240D Med Sci I, Irvine, CA 92697 1700, USA
    Chromosome Res 10:267-77. 2002
    ..of the structural maintenance of chromosomes (SMC) family proteins, hSMC1 and hSMC3, and non-SMC components, hRAD21 and SA1 (or SA2)...
  42. ncbi Cell cycle mechanisms of sister chromatid separation; roles of Cut1/separin and Cut2/securin
    M Yanagida
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Genes Cells 5:1-8. 2000
    ..In the budding yeast, Esp1/separin is thought to be a component of proteolysis against Scc1, an essential subunit of cohesin which is thought to link duplicated sister chromatids up to the anaphase...
  43. ncbi Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I
    María Teresa Parra
    Departamento de Biologia, Edificio de Biológicas, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Cell Sci 117:1221-34. 2004
    ..We have analysed the behaviour of SCP3 and the cohesin subunit Rad21 in mouse spermatocytes by means of a squashing technique...
  44. doi Identification of an ISR-related metabolite produced by rhizobacterium Klebsiella oxytoca C1036 active against soft-rot disease pathogen in tobacco
    Myung Ryeol Park
    Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally Friendly Agriculture Research Centre, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500 757, Republic of Korea
    Pest Manag Sci 65:1114-7. 2009
    ..carotovorum SCC1 (SCC1). However, microbial metabolites from C1036 involved in ISR activity remain unknown...
  45. ncbi Cohesin's ATPase activity is stimulated by the C-terminal Winged-Helix domain of its kleisin subunit
    Prakash Arumugam
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 16:1998-2008. 2006
    ..Whereas Smc1 and Smc3 heterodimerize via their hinge domains, the kleisin subunit Scc1 connects their ATPase heads, and this results in the formation of a large ring...
  46. ncbi The Drosophila RAD21 cohesin persists at the centromere region in mitosis
    W D Warren
    Peter MacCallum Cancer Institute, St Andrews Place, East, Victoria 3002, Melbourne, Australia
    Curr Biol 10:1463-6. 2000
    ..We conclude that in Drosophila, and possibly all metazoans, a minor pool of cohesin remains bound to centromere-proximal chromatin after prophase and maintains sister-chromatid cohesion until the metaphase-anaphase transition...
  47. ncbi Transcription of cholesterol side-chain cleavage cytochrome P450 in the placenta: activating protein-2 assumes the role of steroidogenic factor-1 by binding to an overlapping promoter element
    Micha Ben-Zimra
    Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel 91904
    Mol Endocrinol 16:1864-80. 2002
    ..To address this possibility, we used SCC1, a well conserved proximal region in the P450scc genes (-60/-32 in the rat gene) to purify PNF from human term ..
  48. ncbi Length control of the metaphase spindle
    Gohta Goshima
    Physiology Course 2004, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Curr Biol 15:1979-88. 2005
    ..Although a number of molecular perturbations have been shown to influence spindle length, a global understanding of the factors that determine metaphase spindle length has not been achieved...
  49. pmc In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiae
    John Mc Intyre
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 26:3783-93. 2007
    ..The circumference consists of the Smc1 and Smc3 subunits, while Scc1 is thought to close the ring by bridging the Smc (structural maintenance of chromosomes) ATPase head domains...
  50. pmc Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons
    Andrea Pauli
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Dev Cell 14:239-51. 2008
    ..At the metaphase to anaphase transition, proteolytic cleavage of the alpha kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid ..
  51. pmc Overexpression of Separase induces aneuploidy and mammary tumorigenesis
    Nenggang Zhang
    Department of Pediatric Hematology Oncology, Texas Children s Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:13033-8. 2008
    Separase is an endopeptidase that separates sister chromatids by cleaving cohesin Rad21 during the metaphase-to-anaphase transition...
  52. doi Suppression of interleukin 6 and 8 production in head and neck cancer cells with curcumin via inhibition of Ikappa beta kinase
    Alen N Cohen
    Division of Head and Neck Surgery, David Geffen School of Medicine, University of California, Los Angeles, 10833 Le Conte Ave, Los Angeles, CA 90095 1624, USA
    Arch Otolaryngol Head Neck Surg 135:190-7. 2009
    ..Interleukin 6 and IL-8 are cytokines induced by NF-kappa beta activation with elevated levels in the serum of patients with HNSCC...
  53. doi Cohesion establishment factor, Eco1 represses transcription via association with histone demethylase, LSD1
    Hyun Kyung Choi
    Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440 746, Republic of Korea
    Biochem Biophys Res Commun 394:1063-8. 2010
    ..Eco1 acetylates Smc3 and Mcd1 (Rad21) to establish cohesion during S phase and in response to DNA damage, respectively...
  54. doi Hos1 is a lysine deacetylase for the Smc3 subunit of cohesin
    Bo Xiong
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Curr Biol 20:1660-5. 2010
    ..is established during S phase, and this process requires the four subunits of the cohesin complex (Smc1, Smc3, Mcd1/Scc1, and Irr1/Scc3) and the acetyltransferase Eco1 [10-13]...
  55. ncbi A model for ATP hydrolysis-dependent binding of cohesin to DNA
    Stefan Weitzer
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Curr Biol 13:1930-40. 2003
    ..The mechanism by which cohesin binds to DNA, and how sister chromatid cohesion is established, is unknown...
  56. ncbi Separase-mediated cleavage of cohesin at interphase is required for DNA repair
    Koji Nagao
    Department of Biophysics, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nature 430:1044-8. 2004
    ..10) mutants were additive with cut2(EA2), whereas recombination repair rhp51 (ref. 11) and cohesin subunit rad21 (ref. 12) mutants were not. Cohesin was hyper-modified on ultraviolet irradiation in a Rad3 kinase-dependent way...
  57. pmc Cohesin is dispensable for centromere cohesion in human cells
    Laura A Diaz Martinez
    Department of Genetics, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
    PLoS ONE 2:e318. 2007
    ..Proper regulation of the cohesion at the centromeres of human chromosomes is essential for accurate genome transmission. Exactly how cohesion is maintained and is then dissolved in anaphase is not understood...
  58. pmc Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast
    T Takeda
    Departments of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Mol Biol Cell 12:1257-74. 2001
    ..of proper chromatin structures during mitotic S phase through regulating the functions of Rad3 (ATM)-Cds1 and Rad21 (cohesin), respectively, in addition to expected essential roles for initiation of mitotic DNA replication through ..
  59. ncbi Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O. hubbsi
    C Sakai
    Laboratory of Molecular and Cellular Interactions, Faculty of Advanced Life Science, Hokkaido University, Sapporo, 060 0810, Japan
    Chromosome Res 15:697-709. 2007
    ..gamma-tubulin, inner centromere protein, Cdc20, Mad2, phospho-histone H3 and cohesin subunits (SMC1alpha, SMC3 and Rad21) showed that the expression patterns of these proteins in the hybrid embryos are similar to those in the wild-type ..
  60. pmc Roles of human AND-1 in chromosome transactions in S phase
    Naoko Yoshizawa-Sugata
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    J Biol Chem 284:20718-28. 2009
    ..Furthermore, AND-1 interacts with cohesin proteins Smc1, Smc3, and Rad21/Scc1, consistent with proposed roles of yeast counterparts of AND-1 in sister chromatid cohesion...
  61. ncbi Fate of embryonal carcinoma cells injected into postimplantation mouse embryos
    Simonetta Astigiano
    S S Animali Transgenici, Istituto Nazionale per la Ricerca sul Cancro, Largo Rosanna Benzi 10, 16132 Genova, Italy
    Differentiation 73:484-90. 2005
    ..embryonic environment to "normalize" tumorigenic cells, and to study their homing, we transplanted F9, Nulli-SCC1, and P19 EC cells into 8 to 15-day allogenic CD1 mouse embryos, into allogenic CD1 newborns, and into syngeneic ..
  62. doi Malignant pleural mesothelioma: genome-wide expression patterns reflecting general resistance mechanisms and a proposal of novel targets
    Oluf Dimitri Røe
    Department of Oncology, St Olavs Hospital, Trondheim, Norway
    Lung Cancer 67:57-68. 2010
    ..We propose CHEK1, RAD21, FANCD2 and RAN as new co-targets for mesothelioma treatment...
  63. doi Plant-specific protein MCD1 determines the site of chloroplast division in concert with bacteria-derived MinD
    Hiromitsu Nakanishi
    Initiative Research Program, Advanced Science Institute, RIKEN, Wako, Saitama 351 0198, Japan
    Curr Biol 19:151-6. 2009
    ..Here we show that a plant-specific protein MULTIPLE CHLOROPLAST DIVISION SITE 1 (MCD1) regulates FtsZ ring positioning in Arabidopsis thaliana chloroplasts...
  64. pmc ATP7B detoxifies silver in ciliated airway epithelial cells
    Aida Ibricevic
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Toxicol Appl Pharmacol 243:315-22. 2010
    ..Toward this goal, we previously developed a methylated caffeine silver acetate compound, SCC1, that exhibits broad-spectrum antimicrobial activity against clinical strains of bacteria in vitro and when ..
  65. doi Centriole separation in DNA damage-induced centrosome amplification
    Chiara Saladino
    Centre for Chromosome Biology, Department of Biochemistry and NCBES, National University of Ireland Galway, University Road, Galway, Ireland
    Environ Mol Mutagen 50:725-32. 2009
    ..amplification occurs after ionising radiation (IR) in chicken DT40 cells that lack DNA-PK, Ku70, H2AX, Xpa, and Scc1, demonstrating that these activities are not required for centrosome amplification...
  66. ncbi Mouse CD1 is mainly expressed on hemopoietic-derived cells
    L Brossay
    Department of Microbiology and Immunology, University of California, Los Angeles 90095, USA
    J Immunol 159:1216-24. 1997
    The mouse CD1 (mCD1) is a class I-like molecule that is encoded outside the MHC. Recent studies demonstrate that mCD1 presents hydrophobic peptides to CD8+ T cells and also that it is recognized by a population of NK1...
  67. ncbi Securin is required for chromosomal stability in human cells
    P V Jallepalli
    The Johns Hopkins Oncology Center, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cell 105:445-57. 2001
    ..in the activation of separin, the sister-separating protease, rendering it unable to cleave the cohesin subunit Scc1 efficiently...
  68. ncbi Separase is required for chromosome segregation during meiosis I in Caenorhabditis elegans
    M F Siomos
    Research Institute of Molecular Pathology IMP, Dr Bohrgasse 7, Vienna 1030, Austria
    Curr Biol 11:1825-35. 2001
    ..mechanism removes cohesin along chromosome arms during prophase, while the proteolytic cleavage of cohesin's Scc1 subunit by separase removes centromeric cohesin at anaphase...
  69. ncbi SRC1: an intron-containing yeast gene involved in sister chromatid segregation
    Susana Rodriguez-Navarro
    Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E 46100 Burjassot, Spain
    Yeast 19:43-54. 2002
    ..Deletion of the gene induces a shortening of the anaphase and aggravates the phenotype of scc1 and esp1 conditional mutants, which suggests a direct role of the protein in sister chromatid separation.
  70. ncbi Characterization of gene expression profile associated with energy restriction-induced cold tolerance of heart
    Yasuhiko Wada
    Department of Hygiene, Hyogo College of Medicine, Nishinomiya, 663 8501, Japan
    Microsc Res Tech 59:313-6. 2002
    ..Gene expression analysis using cDNA array was useful for screening genes associated with ER-induced cold adaptation...
  71. ncbi Cohesin component dynamics during meiotic prophase I in mammalian oocytes
    Ignacio Prieto
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, UAM Campus de Cantoblanco, Madrid E 28049, Spain
    Chromosome Res 12:197-213. 2004
    ..Mitotic cohesin subunit RAD21 is also found in fibers that decorate the SC during prophase I in mouse oocytes, suggesting a role for this ..
  72. ncbi Drosophila cohesins DSA1 and Drad21 persist and colocalize along the centromeric heterochromatin during mitosis
    Ana Valdeolmillos
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, UAM Campus de Cantoblanco, Madrid 28049, Spain
    Biol Cell 96:457-62. 2004
    ..Drad21 and DSA1 are the Drosophila homologues of the yeast Scc1 and Scc3 cohesin subunits, respectively...
  73. ncbi Allelic association of the human homologue of the mouse modifier Ptprj with breast cancer
    Fabienne Lesueur
    Cancer Research UK Human Cancer Genetics Research Group, Department of Oncology, University of Cambridge, UK
    Hum Mol Genet 14:2349-56. 2005
    ..tyrosine phosphatase receptor type J (PTPRJ) has been recently identified as being the protein encoded by the Scc1 mouse gene (susceptibility to colon cancer-1)...
  74. ncbi The spindle midzone microtubule-associated proteins Ase1p and Cin8p affect the number and orientation of astral microtubules in Saccharomyces cerevisiae
    Armand de Gramont
    The Laboratory of Molecular and Cellular Biology, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell Cycle 6:1231-41. 2007
    ..When budding yeast cells express the non-cleavable cohesin subunit, Scc1-RRDD, sister chromatid separation is blocked, preventing the spindle from elongating...
  75. doi Identification and characterization of a type III secretion system in Chlamydophila psittaci
    Delphine Sylvie Anne Beeckman
    Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
    Vet Res 39:27. 2008
    ..Unfortunately, SctN was not investigated with this technique. Additionally, we sequenced 14 full-length T3S genes (scc1, sctW, sctJ, sctL, sctR, sctS, scc2, copD1, sctN, sctQ, sctC, incA, ca037, and cadd) and examined the transcription ..
  76. pmc Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast
    Rudra Narayan Dubey
    Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India
    J Biol Chem 284:7165-76. 2009
    ..isopeptide ligase (E3 ligase) followed by degradation of Cut2 releases Cut1, which cleaves the Rad21 subunit of Cohesin, facilitating sister chromatid separation during mitosis...
  77. doi Expression of cohesin and condensin genes during zebrafish development supports a non-proliferative role for cohesin
    Maren Mönnich
    Department of Pathology, The University of Otago, Dunedin, New Zealand
    Gene Expr Patterns 9:586-94. 2009
    ..Cohesin genes smc1a, rad21, pds5b and smc3 were expressed in the forebrain ventricular zone, the tectum, the mid-hindbrain boundary, the ..
  78. pmc Characterization of viral and human RNAs smaller than canonical MicroRNAs
    Zhihua Li
    Department of Computational Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Virol 83:12751-8. 2009
    ..a distinct, unusually small (17-base) RNA (usRNA) that can effectively downregulate a K12-1 miRNA target, human RAD21, suggesting that stable degradation-like products may also contribute to gene regulation...
  79. doi Many functions of the meiotic cohesin
    Amit Bardhan
    Chromosome Res 18:909-24. 2010
    ..In meiosis of most organisms, the mitotic Mcd1/Scc1/Rad21 subunit of the cohesin complex is largely replaced by its paralog named Rec8...
  80. pmc Large scale RNAi screen reveals that the inhibitor of DNA binding 2 (ID2) protein is repressed by p53 family member p63 and functions in human keratinocyte differentiation
    Ning Wu
    CEA, iRTSV, Laboratoire Biopuces, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    J Biol Chem 286:20870-9. 2011
    ..Three genes NBN, RAD21, and p63 lead to a repression of ID2 promoter activity...
  81. ncbi Comparison of clinicopathological features and hRad21 expression between telomerase-dependent and telomerase-independent colorectal cancer
    Bing Qiang Yi
    Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China
    Hepatogastroenterology 58:785-9. 2011
    To investigate the difference of clinicopathological features and expression of hRad21 between telomerase-dependent and telomerase-independent colorectal cancer (CRC).
  82. pmc Regulation of sororin by Cdk1-mediated phosphorylation
    Megan R Dreier
    Department of Biological Sciences, University of Toledo, 2801 W Bancroft Street, MS 601, Toledo, OH 43606, USA
    J Cell Sci 124:2976-87. 2011
    ..Cohesion of sister chromatids is maintained by the multi-protein complex cohesin, consisting of Smc1, Smc3, Scc1 and Scc3. Sororin associates with the cohesin complex and regulates the segregation of sister chromatids...
  83. pmc APRIN is a cell cycle specific BRCA2-interacting protein required for genome integrity and a predictor of outcome after chemotherapy in breast cancer
    Rachel Brough
    Cancer Research UK Gene Function and Regulation Group, London, UK
    EMBO J 31:1160-76. 2012
    ....
  84. doi The specific contributions of cohesin-SA1 to cohesion and gene expression: implications for cancer and development
    Ana Cuadrado
    Chromosome Dynamics Group, Molecular Oncology Programme, Spanish National Cancer Research Centre, Madrid, Spain
    Cell Cycle 11:2233-8. 2012
    ..Vertebrate somatic cells have two different versions of cohesin, containing Smc1, Smc3, Rad21/Scc1 and either SA1 or SA2, but their functional specificity has been largely ignored...
  85. doi Cellular localization of mitotic RAD21 with repetitive amino acid motifs in Allium cepa
    Go Suzuki
    Division of Natural Science, Osaka Kyoiku University, 4 698 1 Asahigaoka, Kashiwara, Osaka 582 8582, Japan
    Gene 514:75-81. 2013
    ..Here we cloned and characterized an onion homologue of mitotic RAD21 gene, AcRAD21-1, to develop a molecular marker of mitosis...
  86. pmc Synthesis, characterization, and in vivo efficacy of shell cross-linked nanoparticle formulations carrying silver antimicrobials as aerosolized therapeutics
    Parth N Shah
    Department of Pediatrics, Division of Pulmonary and Vascular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    ACS Nano 7:4977-87. 2013
    ..Ag(+) delivered over 5 doses at 12 h intervals was necessary to achieve a 60% survival advantage with a free-drug (SCC1) formulation...
  87. pmc Functional characterization of NIPBL physiological splice variants and eight splicing mutations in patients with Cornelia de Lange syndrome
    María E Teresa-Rodrigo
    Unit of Clinical Genetics and Functional Genomics, Departments of Pharmacology Physiology and Pediatrics, School of Medicine, University of Zaragoza, E 50009 Zaragoza, Spain
    Int J Mol Sci 15:10350-64. 2014
    ..Mutations in five genes encoding structural components (SMC1A, SMC3, RAD21) or functionally associated factors (NIPBL, HDAC8) of the cohesin complex have been found in patients with CdLS...
  88. pmc Compromised structure and function of HDAC8 mutants identified in Cornelia de Lange Syndrome spectrum disorders
    Christophe Decroos
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323 United States
    ACS Chem Biol 9:2157-64. 2014
    ..resulting from mutations in genes that encode the core components of the cohesin complex, SMC1A, SMC3, and RAD21, or two of its regulatory proteins, NIPBL and HDAC8...
  89. pmc Depletion of the chromatin looping proteins CTCF and cohesin causes chromatin compaction: insight into chromatin folding by polymer modelling
    Mariliis Tark-Dame
    Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
    PLoS Comput Biol 10:e1003877. 2014
    ..supra Mb) folding of human chromosomes, we knocked down the gene that codes for CTCF and the one coding for Rad21, an essential subunit of cohesin...
  90. doi Broadening of cohesinopathies: exome sequencing identifies mutations in ANKRD11 in two patients with Cornelia de Lange-overlapping phenotype
    I Parenti
    Medical Genetics, Department of Health Sciences, Universita degli Studi di Milano, Milan, Italy
    Clin Genet 89:74-81. 2016
    ..Mutations in one of the five genes NIPBL, SMC1A, SMC3, HDAC8 or RAD21, were identified in at least 70% of the patients with CdLS...
  91. doi Enhanced GAB2 Expression Is Associated with Improved Survival in High-Grade Serous Ovarian Cancer and Sensitivity to PI3K Inhibition
    Sally J Davis
    Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia
    Mol Cancer Ther 14:1495-503. 2015
    ..Tissue Analysis cohort for 11 copy number amplified genes: ATP13A4, BMP8B, CACNA1C, CCNE1, DYRK1B, GAB2, PAK4, RAD21, TPX2, ZFP36, and URI...
  92. pmc Chlamydia trachomatis Type III Secretion Proteins Regulate Transcription
    Brett R Hanson
    Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA
    J Bacteriol 197:3238-44. 2015
    ..are connected, we first investigated physical interactions between Chlamydia trachomatis Scc4 and the T3S chaperone Scc1 and a T3S substrate, CopN...
  93. pmc Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms
    Jasper Mullenders
    Howard Hughes Medical Institute, Department of Pathology, and Center for Health Informatics and Bioinformatics, School of Medicine and Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY 10016 Howard Hughes Medical Institute, Department of Pathology, and Center for Health Informatics and Bioinformatics, School of Medicine and Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY 10016
    J Exp Med 212:1833-50. 2015
    The cohesin complex (consisting of Rad21, Smc1a, Smc3, and Stag2 proteins) is critically important for proper sister chromatid separation during mitosis...
  94. doi Prenatal diagnosis and array comparative genomic hybridization characterization of interstitial deletions of 8q23.3-q24.11 and 8q24.13 associated with Langer-Giedion syndrome, Cornelia de Lange syndrome and haploinsufficiency of TRPS1, RAD21 and EXT1
    Chih Ping Chen
    Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan Department of Biotechnology, Asia University, Taichung, Taiwan School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan Institute of Clinical and Community Health Nursing, National Yang Ming University, Taipei, Taiwan Department of Obstetrics and Gynecology, School of Medicine, National Yang Ming University, Taipei, Taiwan Electronic address
    Taiwan J Obstet Gynecol 54:592-6. 2015
    The aim of this research was to present prenatal diagnosis of Langer-Giedion syndrome (LGS/TRPS type II) and Cornelia de Lange syndrome-4 (CDLS4).
  95. ncbi Degradation of a cohesin subunit by the N-end rule pathway is essential for chromosome stability
    H Rao
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Nature 410:955-9. 2001
    ..the metaphase-anaphase transition in the yeast Saccharomyces cerevisiae, the ESP1-encoded protease separin cleaves SCC1, a subunit of cohesin with a relative molecular mass of 63,000 (Mr 63K)...
  96. ncbi A nucleus-encoded suppressor defines a new factor which can promote petD mRNA stability in the chloroplast of Chlamydomonas reinhardtii
    D Esposito
    Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, NY 14853, USA
    Curr Genet 39:40-8. 2001
    Mutations in the Chlamydomonas reinhardtii nuclear gene MCD1 specifically destabilize the chloroplast petD mRNA, which encodes subunit IV of the cytochrome b6/f complex...
  97. ncbi Requirement of heterochromatin for cohesion at centromeres
    P Bernard
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Science 294:2539-42. 2001
    ..During cell division, newly replicated sister chromatids must cohere until anaphase when Scc1/Rad21-mediated cohesion is destroyed...
  98. ncbi Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest
    Shao Win Wang
    Imperial Cancer Research Fund Molecular Oncology Laboratory, University of Oxford Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, UK
    J Cell Sci 115:587-98. 2002
    ..The S. pombe Pds5 protein was localised to punctate nuclear foci in a manner that was dependent on the Rad21 cohesin component. This, together with additional genetic evidence, points towards an involvement of S...
  99. ncbi Dicer is essential for formation of the heterochromatin structure in vertebrate cells
    Tatsuo Fukagawa
    Precursory Research for Embryonic Science and Technology of Japan Science and Technology Agency, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 6:784-91. 2004
    ..Immunocytochemical analysis revealed abnormalities in the localization of two heterochromatin proteins, Rad21 cohesin protein and BubR1 checkpoint protein, but the localization of core kinetochore proteins such as centromere ..
  100. ncbi Regulation of Separase in meiosis: Separase is activated at the metaphase I-II transition in Xenopus oocytes during meiosis
    Heng Yu Fan
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Cycle 5:198-204. 2006
    ..Purified xSeparase can cleave the human alpha-kleisin subunit of cohesin in vitro but cannot bind to hSecurin when these two proteins are coexpressed in 293T cells...
  101. ncbi Evidence that loading of cohesin onto chromosomes involves opening of its SMC hinge
    Stephan Gruber
    Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 127:523-37. 2006
    ..N- and C-terminal domains within cohesin's kleisin subunit Scc1 bind to Smc3 and Smc1 ATPase heads respectively, thereby creating a huge tripartite ring...

Research Grants19

  1. Matthew J O'Connell; Fiscal Year: 2015
    ..Moreover, the same collection of rad mutants includes the cohesin gene rad21. Further, Smc5/6 is essential for cell viability, but DNA repair genes are not...
  2. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
    ..Sgo1 protects centromeric cohesin (Scc1/RAD21). BUB1 regulates the stability and centromeric localization of SGO1 in human cells...
  3. Paul M Lieberman; Fiscal Year: 2015
    ..g. smc1, smc3, rad21)...
  4. The Cytogenetics of Meiosis of Maize
    WILLIAM ZACHEUS CANDE; Fiscal Year: 2010
    ..We will use ChIP on tiling arrays to identify histone modifications and RAD21/REC8 binding sites in a defined chromosome region...
  5. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2010
    ..These genes are divided into three groups: structural factors, made up of the four-subunit cohesin complex (Smc1, Scc1, Smc3 and Scc3), which holds sister chromatids together after replication...
  6. Ann J Feeney; Fiscal Year: 2014
    ..To demonstrate whether CTCF and/or cohesin are necessary for IgH locus contraction, we will knock down CTCF or Rad21 in pro-B cells and assess whether locus contraction is affected using 3D-FISH...
  7. Molecular Basis of Aneuploidy
    Debananda Pati; Fiscal Year: 2010
    ..ESPL1, which encodes an endopeptidase called Separase that separates joined sister chromatids by cleaving cohesin Rad21/SCC1/MCD1 during the metaphase to anaphase transition...
  8. Role of MRK in DNA Damage Response
    Rosamaria Ruggieri; Fiscal Year: 2007
    ..The ATM-Chk2/Chkl-Cdc25A cascade, downstream of the BRCT-motif-containing proteins MCD1, Nbsl and 53BP1, plays an important role in gamma-radiation-induced cell cycle arrest in S and G2 phase...
  9. Antigen Presentation Pathway by CD1 Molecules
    Mitchell Kronenberg; Fiscal Year: 2006
    ..restricted intracellular trafficking, and carry out cell fractionation studies to determine where processing and mCD1 antigen binding occur...
  10. Mitotic Regulation of Apoptosis in Leukemia
    Debananda Pati; Fiscal Year: 2010
    ..Cohesin Rad21, a mitotic regulatory protein may play an important role in the interface between cell proliferation and apoptosis...
  11. Antigen Presentation Pathway by CD1 Molecules
    PING LAWTON; Fiscal Year: 2004
    ..We will analyze the intracellular trafficking of antigens and mCD1 d, glycolipid antigen processing, and the in vivo effects on NKT cells resulting from the alteration of the antigen ..
  12. Peptide Morphing: Rational Design of Separase Inhibitors
    Webster Santos; Fiscal Year: 2005
    Separase has recently been identified as a cysteine protease that cleaves the Scc1 subunit of cohesin, a complex protein that physically connects sister chromatids and serves important functions in mitotic spindle dynamics...
  13. PDS5P IN SISTER CHROMATID COHESION
    Vincent Guacci; Fiscal Year: 2005
    ..The types of genetic instability generated by mutations in pds5 and mcd1 will be quantified by measuring unequal sister chromatid recombination and sensitivity to DNA damaging agents...
  14. ANTIGEN PRESENTATION AND IMMUNE REGULATION BY CD1
    Mitchell Kronenberg; Fiscal Year: 2004
    ..1 (mCD1), in order to define how mCD1 can stimulate autoreactive, T cells and present both lipid and peptide antigens...
  15. Analysis of Cdc20-Mad2 in spindle checkpoint signalling
    CLINTON COPELAND; Fiscal Year: 2006
    ..We will use temperature sensitive mcd1-1 mutants to screen for genes involved in this process...
  16. Analyses of sigma factor 28 of C. trachomatis
    Li Shen; Fiscal Year: 2010
    ..Information derived from these studies may lead to a better understanding of global regulation of genes, intracellular parasitism, virulence, immune evasion and persistence of C. trachomatis. ..
  17. YEAST CHROMOSOME STRUCTURE, REPLICATION AND SEGREGATION
    Angelika Amon; Fiscal Year: 2004
    ..Our objective for this meeting is to continue the extremely high level of excitement and scientific excellence that has characterized the past seven conferences. ..
  18. THE ROLE OF APOPTOSIS IN CF PSEUDOMONAL LUNG INFECTION
    CAROLYN CANNON; Fiscal Year: 2004
    ..abstract_text> ..
  19. Silver carbene complexes as treatment of CF lung disease
    CAROLYN CANNON; Fiscal Year: 2008
    ..abstract_text> ..