PYGM

Summary

Gene Symbol: PYGM
Description: glycogen phosphorylase, muscle associated
Alias: glycogen phosphorylase, muscle form, myophosphorylase, phosphorylase, glycogen, muscle
Species: human

Top Publications

  1. ncbi Role of the sympathoadrenal system in the regulation of glycogen metabolism in resting and exercising skeletal muscles
    C Lavoie
    Research Group on Diabetes and Metabolic Regulation IRCM (Clinical Research Institute of Montreal, , Canada
    Horm Metab Res 24:266-71. 1992
  2. ncbi Hypogammaglobulinemia in McArdle myopathy (glycogenosis type V)
    M E Westarp
    Ulm University, Department of Neurology, Federal Republic of Germany
    Eur Arch Psychiatry Clin Neurosci 240:159-62. 1991
  3. ncbi Phosphorylase and creatine kinase modification by thiol-disulfide exchange and by xanthine oxidase-initiated S-thiolation
    R M Miller
    Department of Biochemistry and Biophysics, Iowa State University, Ames 50011
    Arch Biochem Biophys 276:355-63. 1990
  4. ncbi Freezing tolerance/intolerance and cryoprotectant synthesis in terrestrially overwintering anurans in the Great Plains, USA
    D L Swanson
    Department of Biology, University of South Dakota, Vermillion 57069 239, USA
    J Comp Physiol B 166:110-9. 1996
  5. ncbi Stimulation by anaphylatoxin C5a of glycogen phosphorylase in rat hepatocytes via prostanoid release from hepatic stellate cells but not sinusoidal endothelial cells
    H L Schieferdecker
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat Gottingen, Germany
    FEBS Lett 434:245-50. 1998
  6. ncbi Molecular diagnosis of McArdle disease: revised genomic structure of the myophosphorylase gene and identification of a novel mutation
    C Kubisch
    Center for Molecular Neurobiology, University of Hamburg, Germany
    Hum Mutat 12:27-32. 1998
  7. ncbi Molecular genetic heterogeneity of myophosphorylase deficiency (McArdle's disease)
    S Tsujino
    H Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Diseases, Department of Neurology, Columbia Presbyterian Medical Center, New York, NY 10032
    N Engl J Med 329:241-5. 1993
  8. ncbi Expression of the muscle glycogen phosphorylase gene in patients with McArdle disease: the role of nonsense-mediated mRNA decay
    Gisela Nogales-Gadea
    Departament de Patologia Mitocondrial i Neuromuscular, Institut de Recerca, Hospital Universitari Vall d Hebron, Barcelona, Spain
    Hum Mutat 29:277-83. 2008
  9. doi Novel mutations in patients with McArdle disease by analysis of skeletal muscle mRNA
    I Garcia-Consuegra
    Centro de Investigacion, Hospital Universitario 12 de Octubre, Avda Cordoba s n, 28041 Madrid, Spain
    J Med Genet 46:198-202. 2009
  10. ncbi Enzymic pattern of preneoplastic and neoplastic lesions induced in the kidney of CBA mice by 1,2-dimethylhydrazine
    Y S Ahn
    , Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Toxicol Pathol 22:415-22. 1994

Research Grants

  1. EVOLUTION OF ALLOSTERIC REGULATION IN PHOSPHORYLASE
    Robert Fletterick; Fiscal Year: 1999
  2. SUBUNIT INTERACTIONS OF PHOSPHORYLASE KINASE
    Gerald M Carlson; Fiscal Year: 2013
  3. Gerald M Carlson; Fiscal Year: 2014
  4. ENZYMIC AND NON-ENZYMIC GLYCOSYL TRANSFER
    Michael Sinnott; Fiscal Year: 1993
  5. STRUCTURE-FUNCTION RELATIONSHIPS IN PHOSPHORYLASE A
    Robert Fletterick; Fiscal Year: 1993
  6. HORMONAL REGULATION OF PHOSPHOINOSITIDE METABOLISM
    JOHN FAIN; Fiscal Year: 1991
  7. CONTROL OF PEPTIDE HORMONE LEVELS IN INSECTS
    Rolf Ziegler; Fiscal Year: 1991
  8. GLUCOCORTICOID PERMISSIVE ACTION ON LIVER METABOLISM
    Timothy Chan; Fiscal Year: 1980
  9. GUANIDINE SPECIFIC MONO ADP-RIBOSYLATION REACTIONS
    Donald Graves; Fiscal Year: 1990
  10. CONTROL OF ENZYME BIOSYNTHESIS--ROLE OF CYCLIC AMP
    HOWARD RICKENBERG; Fiscal Year: 1980

Detail Information

Publications316 found, 100 shown here

  1. ncbi Role of the sympathoadrenal system in the regulation of glycogen metabolism in resting and exercising skeletal muscles
    C Lavoie
    Research Group on Diabetes and Metabolic Regulation IRCM (Clinical Research Institute of Montreal, , Canada
    Horm Metab Res 24:266-71. 1992
    ..These data indicate that catecholamines do not play a primary role in skeletal muscle glycogen breakdown during acute exercise and suggest that allosteric regulators may be of prime importance...
  2. ncbi Hypogammaglobulinemia in McArdle myopathy (glycogenosis type V)
    M E Westarp
    Ulm University, Department of Neurology, Federal Republic of Germany
    Eur Arch Psychiatry Clin Neurosci 240:159-62. 1991
    ..It is characterized by a myophosphorylase defect...
  3. ncbi Phosphorylase and creatine kinase modification by thiol-disulfide exchange and by xanthine oxidase-initiated S-thiolation
    R M Miller
    Department of Biochemistry and Biophysics, Iowa State University, Ames 50011
    Arch Biochem Biophys 276:355-63. 1990
    ..For proteins typified by phosphorylase b, the accumulation of S-thiolated forms may be more independent of GSH...
  4. ncbi Freezing tolerance/intolerance and cryoprotectant synthesis in terrestrially overwintering anurans in the Great Plains, USA
    D L Swanson
    Department of Biology, University of South Dakota, Vermillion 57069 239, USA
    J Comp Physiol B 166:110-9. 1996
    ..Thus, P. triseriata, but not B. cognatus, exhibited winter increment of liver phosphorylase activity that is correlated with the development of freezing tolerance...
  5. ncbi Stimulation by anaphylatoxin C5a of glycogen phosphorylase in rat hepatocytes via prostanoid release from hepatic stellate cells but not sinusoidal endothelial cells
    H L Schieferdecker
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat Gottingen, Germany
    FEBS Lett 434:245-50. 1998
    ....
  6. ncbi Molecular diagnosis of McArdle disease: revised genomic structure of the myophosphorylase gene and identification of a novel mutation
    C Kubisch
    Center for Molecular Neurobiology, University of Hamburg, Germany
    Hum Mutat 12:27-32. 1998
    ..Until now, a total number of 11 different mutations in the coding region or splice sites of the myophosphorylase gene have been identified...
  7. ncbi Molecular genetic heterogeneity of myophosphorylase deficiency (McArdle's disease)
    S Tsujino
    H Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Diseases, Department of Neurology, Columbia Presbyterian Medical Center, New York, NY 10032
    N Engl J Med 329:241-5. 1993
    b>Myophosphorylase deficiency (McArdle's disease) is one of the most common causes of exercise intolerance, muscle cramps, and recurrent myoglobinuria...
  8. ncbi Expression of the muscle glycogen phosphorylase gene in patients with McArdle disease: the role of nonsense-mediated mRNA decay
    Gisela Nogales-Gadea
    Departament de Patologia Mitocondrial i Neuromuscular, Institut de Recerca, Hospital Universitari Vall d Hebron, Barcelona, Spain
    Hum Mutat 29:277-83. 2008
    Nearly 35% of all mutations identified in the muscle glycogen phosphorylase gene (PYGM) in patients with McArdle disease result in premature termination codons (PTCs), particularly the p.R50X mutation...
  9. doi Novel mutations in patients with McArdle disease by analysis of skeletal muscle mRNA
    I Garcia-Consuegra
    Centro de Investigacion, Hospital Universitario 12 de Octubre, Avda Cordoba s n, 28041 Madrid, Spain
    J Med Genet 46:198-202. 2009
    To identify pathogenic mutant alleles of the PYGM gene in "genetic manifesting heterozygous" patients with McArdle disease-that is, those in whom we could only find a sole mutant allele by genomic DNA analysis.
  10. ncbi Enzymic pattern of preneoplastic and neoplastic lesions induced in the kidney of CBA mice by 1,2-dimethylhydrazine
    Y S Ahn
    , Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Toxicol Pathol 22:415-22. 1994
    ....
  11. ncbi High frequency of missense mutations in glycogen storage disease type VI
    N J Beauchamp
    Academic Unit of Child Health, University of Sheffield, Stephenson Wing, Sheffield Children s NHS Trust, Western Bank, Sheffield, S10 2TH, UK
    J Inherit Metab Dis 30:722-34. 2007
    ..mutations compared to 68-80% of affected alleles of the highly homologous muscle glycogen phosphorylase gene, PYGM, that underlie McArdle disease. There was heterogeneity in the clinical symptoms observed in affected individuals...
  12. doi Effect of changes in fat availability on exercise capacity in McArdle disease
    Susanne T Andersen
    Neuromuscular Research Unit, Department of Neurology, and Copenhagen Muscle Research Center, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Arch Neurol 66:762-6. 2009
    ..The major fuel for exercising muscle at low exercise intensities is fat...
  13. ncbi Glycogen phosphorylase in the fat body of two cockroach species, Periplaneta americana and Nauphoeta cinerea: isolation, partial characterization of three forms and activation by hypertrehalosaemic hormones
    G GADe
    Zoology Department, University of Cape Town, Rondebosch, Republic of South Africa
    Z Naturforsch C 46:149-62. 1991
    ..021, 0.019 and 0.073% for glycogen and 8.3, 6.3 and 17.9 mM for Pi (a-, ab- and b-form). Phosphorylase a exhibits a more or less hyperbolic response to AMP and needs 70 microM AMP for maximal stimulation.(ABSTRACT TRUNCATED AT 400 WORDS)..
  14. ncbi Creatine therapy in myophosphorylase deficiency (McArdle disease): a placebo-controlled crossover trial
    M Vorgerd
    Department of Neurology, Ruhr University Bochum, Kliniken Bergmannsheil, Burkle de la Camp Platz 1, 44789 Bochum, Germany
    Arch Neurol 57:956-63. 2000
    To determine whether treatment with creatine can improve exercise intolerance in myophosphorylase deficiency (McArdle disease).
  15. doi McArdle disease: what do neurologists need to know?
    Alejandro Lucia
    Department of Physiology, Universidad Europea de Madrid, Madrid, Spain
    Nat Clin Pract Neurol 4:568-77. 2008
    ..disease (also known as glycogen storage disease type V) is a pure myopathy caused by an inherited deficit of myophosphorylase, the skeletal muscle isoform of the enzyme glycogen phosphorylase...
  16. ncbi A proposed molecular diagnostic flowchart for myophosphorylase deficiency (McArdle disease) in blood samples from Spanish patients
    Juan C Rubio
    Centro de Investigacion, Hospital Universitario 12 de Octubre, Madrid, Spain
    Hum Mutat 28:203-4. 2007
    McArdle disease is a metabolic myopathy due to molecular defects in the myophosphorylase gene (PYGM), usually diagnosed in muscle biopsy...
  17. ncbi Mitochondrial respiratory mutants of Saccharomyces cerevisiae accumulate glycogen and readily mobilize it in a glucose-depleted medium
    B Enjalbert
    , UMR CNRS 5504, UR INRA 792, , Complexe Scientifique de Rangueil, 31077 Toulouse, France
    Microbiology 146:2685-94. 2000
    ..It is also proposed that this degradation provides the energy for the viability of respiratory mutants in glucose-starved medium...
  18. ncbi Changes in lipid and carbohydrate metabolism during starvation in adult Manduca sexta
    R Ziegler
    , , Federal Republic of Germany
    J Comp Physiol B 161:125-31. 1991
    ....
  19. ncbi Electrical stimulation of the suprachiasmatic nucleus of the hypothalamus causes hyperglycemia
    K Nagai
    Institue for Protein Research, Osaka University, Japan
    Horm Metab Res 20:37-9. 1988
    ..These findings suggest that the SCN is important in control of glucose homeostasis through effects on glucagon and insulin secretions and liver glycogen metabolism...
  20. ncbi Analogous activation of bovine liver glycogen phosphorylase by AMP and IMP
    A Camara-Artigas
    Departamento de Quimica Fisica, Bioquimica y Quimica Inorganica, Facultad de Ciencias Experimentales, Universidad de Almeria, Spain
    Int J Biochem Cell Biol 29:849-56. 1997
    ....
  21. ncbi Response of glucose metabolism enzymes in an acute porphyria model. Role of reactive oxygen species
    Sandra M Lelli
    Salud Humana y Medio Ambiente DIMXSA, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Ciudad Autonoma de Buenos Aires, Argentina
    Toxicology 216:49-58. 2005
    ....
  22. ncbi Muscle fiber distribution, capillary density, and enzymatic activities in the lumbar paravertebral muscles of young men. Significance for isometric endurance
    K Jørgensen
    August Krogh Institute University of Copenhagen, Denmark
    Spine (Phila Pa 1976) 18:1439-50. 1993
    ..No obvious bilateral differences in the histochemical and biochemical results were present. The isometrical trunk extensor endurance time is markedly increased compared to other human skeletal muscles...
  23. ncbi Glucose analog inhibitors of glycogen phosphorylases as potential antidiabetic agents: recent developments
    Laszlo Somsak
    Department of Organic Chemistry, Faculty of Science, Egyetem ter 1, POB 20, H 4010 Debrecen, H 4026 Debrecen, Hungary
    Curr Pharm Des 9:1177-89. 2003
    ..Computational methods suggest to synthesize further anomerically bifunctional glucose derivatives which may be good inhibitors of GP...
  24. ncbi The role of protein kinase A in anaerobic energy production during liver storage
    T A Churchill
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    J Hepatol 32:473-80. 2000
    ....
  25. ncbi Impact of copper on the oxidative metabolism of the fry of common carp, Cyprinus carpio (Linn.) at different pH
    A Sreenivasa Reddy
    Department of Zoology, Sri Krishnadevaraya University, Anantapur 515 003, India
    J Environ Biol 29:721-4. 2008
    ..These results indicated that copper suppresses the energetics of the fish fry at pH 6.0, elevates at pH 9.0 relative to the changes at pH 7.5 suggesting that the toxicity of copper is dependent on pH of the water...
  26. ncbi The content of glycogen phosphorylase and glycogen in preparations of sarcoplasmic reticulum-glycogenolytic complex is enhanced in diabetic rat skeletal muscle
    E Garduno
    Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Extremadura, Badajoz, Spain
    Diabetologia 44:1238-46. 2001
    ..We have examined the effect of diabetes and pharmacological insulin treatment on the content of glycogen phosphorylase and glycogen associated with the sarcoplasmic reticulum-glycogenolytic complex from rat skeletal muscle...
  27. pmc Fatty acid and amino acid modulation of glucose cycling in isolated rat hepatocytes
    L A Gustafson
    Department of Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Biochem J 358:665-71. 2001
    ..Variation in glucose 6-phosphate does not always result in activity changes of GSa. Activation of glucose 6-phosphatase flux by fatty acids may contribute to the increased hepatic glucose production as seen in Type 2 diabetes...
  28. ncbi A putative hyperglycemic factor from the cerebral ganglia of Otala lactea (Mollusca: Pulmonata)
    A M Abdraba
    Department of Biology, York University, Toronto, Ontario, Canada
    J Comp Physiol B 170:219-24. 2000
    ..The molecular weight of this factor, estimated by size-exclusion chromatography, was approximately 10,000. Mammalian glucagon had no significant effect on glycogen synthesis by the mantle pieces...
  29. pmc Control of phosphorylase b conformation by a modified cofactor: crystallographic studies on R-state glycogen phosphorylase reconstituted with pyridoxal 5'-diphosphate
    D D Leonidas
    Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
    Protein Sci 1:1112-22. 1992
    ..The observed position in the crystal suggests that it is too far from the substrate site to represent a site for catalysis...
  30. ncbi Increase by anaphylatoxin C5a of glucose output in perfused rat liver via prostanoids derived from nonparenchymal cells: direct action of prostaglandins and indirect action of thromboxane A(2) on hepatocytes
    H L Schieferdecker
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat Gottingen, Gottingen, Germany
    Hepatology 30:454-61. 1999
    ..Thus, in the perfused rat liver PGs released after C5a-stimulation from KCs and HSCs directly activated glycogen phosphorylase in HCs, and TXA(2) enhanced glucose output indirectly mainly by causing hypoxia as a result of flow reduction...
  31. ncbi Idazoxan activates rat forebrain glycogen phosphorylase in vivo: a histochemical study
    Maria Fara-On
    Psychology Department, Memorial University of Newfoundland, St John s, Canada NL A1B 3X9
    Brain Res 1059:83-92. 2005
    ..They contrast with earlier evidence showing that idazoxan reduces 2-deoxyglucose uptake in these brain areas. Idazoxan, and norepinephrine, may preferentially recruit glycolytic over oxidative metabolism in the rat forebrain...
  32. ncbi Schistosoma haematobium: histochemistry of glycogen, glycogen phosphorylase a and glycogen branching enzyme in niridazole-treated females
    T Moczon
    University of Geneva, Switzerland
    Int J Parasitol 22:55-63. 1992
    ..1.3.17); a decrease in glycogen branching enzyme activity; and a relatively rapid degeneration of parasite cells possibly due to their loss of endogenous energy reserves...
  33. ncbi The structure of glycogen phosphorylase b with an alkyldihydropyridine-dicarboxylic acid compound, a novel and potent inhibitor
    S E Zographos
    Institute of Biological Research and Biotechnology, The National Hellenic Research Foundation 48, vas Constantinou Avenue, Athens, 11635, Greece
    Structure 5:1413-25. 1997
    ..The inhibitor has no structural similarity to the natural regulators of GP. We have carried out structural studies in order to elucidate the mechanism of inhibition...
  34. ncbi Kinetic analysis of the separate phosphorylation events in the phosphorylase kinase reaction
    W R Harris
    Department of Biochemistry and Biophysics, Iowa State University, Ames 50011
    Arch Biochem Biophys 276:102-8. 1990
    ..In this context, the effect of AMP is to reduce the conformational differences between phosphorylases b and ab, and the gamma delta complex is less sensitive to the conformational differences between the two forms of phosphorylase...
  35. ncbi Endocrine control of TAG lipase in the fat body of the migratory locust, Locusta migratoria
    Lutz Auerswald
    Zoology Department, University of Cape Town, Rondebosch 7701, South Africa
    Insect Biochem Mol Biol 36:759-68. 2006
    ....
  36. pmc The regulatory beta subunit of phosphorylase kinase interacts with glyceraldehyde-3-phosphate dehydrogenase
    Igor G Boulatnikov
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Biochemistry 47:7228-36. 2008
    ..The interactions described herein between the beta subunit of PhK and GAPDH provide a possible mechanism for the direct linkage of glycogenolysis and glycolysis in skeletal muscle...
  37. pmc Mutations in the liver glycogen phosphorylase gene (PYGL) underlying glycogenosis type VI
    B Burwinkel
    Institut fur Physiologische Chemie, Ruhr Universitat Bochum, Bochum, Germany
    Am J Hum Genet 62:785-91. 1998
    ..coding sequence, sequence polymorphisms, and a partial PYGL gene structure with introns in the same positions as in PYGM, the gene of the muscle isoform of phosphorylase...
  38. ncbi Liver hyperplasia and paradoxical regulation of glycogen metabolism and glucose-sensitive gene expression in GLUT2-null hepatocytes. Further evidence for the existence of a membrane-based glucose release pathway
    R Burcelin
    Institute of Pharmacology and Toxicology, University of Lausanne, 27, Rue du Bugnon, CH 1005 Lausanne, Switzerland
    J Biol Chem 275:10930-6. 2000
    ..In the absence of this equilibration, the control of hepatic glucose metabolism by G6P is dominant over that by plasma hormone concentrations...
  39. pmc Allosteric inhibition of glycogen phosphorylase a by the potential antidiabetic drug 3-isopropyl 4-(2-chlorophenyl)-1,4-dihydro-1-ethyl-2-methyl-pyridine-3,5,6-tricarbo xylate
    N G Oikonomakos
    Institute of Biological Research and Biotechnology, The National Hellenic Research, Athens, Greece
    Protein Sci 8:1930-45. 1999
    ..The structures provide a rational for the potency of the inhibitor and explain GPa allosteric inhibition of activity upon W1807 binding...
  40. pmc FR258900, a potential anti-hyperglycemic drug, binds at the allosteric site of glycogen phosphorylase
    Costas Tiraidis
    Institute of Organic and Pharmaceutical Chemistry, National Hellenic Research Foundation, Athens, Greece
    Protein Sci 16:1773-82. 2007
    ..The ligand-binding mode is different from those of the potent phenoxy-phthalate and acyl urea inhibitors, previously described, illustrating the broad specificity of the allosteric site...
  41. doi The binding of β-d-glucopyranosyl-thiosemicarbazone derivatives to glycogen phosphorylase: A new class of inhibitors
    Kyra Melinda Alexacou
    Institute of Organic and Pharmaceutical Chemistry, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece
    Bioorg Med Chem 18:7911-22. 2010
    ..In addition, 14 out of the 15 studied inhibitors were found bound at the new allosteric site of the enzyme...
  42. ncbi Adjustments of the enzymatic complement for polyol biosynthesis and accumulation in diapausing cold-acclimated adults of Pyrrhocoris apterus
    V Kostal
    Institute of Entomology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic
    J Insect Physiol 50:303-13. 2004
    ..Different electrophoretic mobilities in PAGE gel suggested that KR and PDH are two different enzymes with specific requirement for NADP(H) or NAD(H), respectively, as co-factors...
  43. ncbi The binding of 2-deoxy-D-glucose 6-phosphate to glycogen phosphorylase b: kinetic and crystallographic studies
    N G Oikonomakos
    Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
    J Mol Biol 254:900-17. 1995
    ....
  44. ncbi Stimulating effects of low-dose fructose on insulin-stimulated hepatic glycogen synthesis in humans
    K F Petersen
    Department of Internal Medicine, Yale University School of Medicine, 333 Cedar St, FMP 104, New Haven, CT 06520 8020, USA
    Diabetes 50:1263-8. 2001
    ..Because net hepatic glycogen synthesis has been shown to be diminished in patients with poorly controlled type 1 and type 2 diabetes, stimulation of hepatic glycogen synthesis by this mechanism may be of potential therapeutic value...
  45. ncbi Structural mechanism for glycogen phosphorylase control by phosphorylation and AMP
    D Barford
    Laboratory of Molecular Biophysics, University of Oxford, U K
    J Mol Biol 218:233-60. 1991
    ..abstract truncated at 400 words)..
  46. ncbi Human liver glycogen phosphorylase inhibitors bind at a new allosteric site
    V L Rath
    Department of Exploratory Medicinal Sciences, Global Research and Development, Pfizer Inc, Groton, CT 06340, USA
    Chem Biol 7:677-82. 2000
    ..Maintaining control of blood glucose levels is critical in minimizing the debilitating effects of diabetes, making liver glycogen phosphorylase a potential therapeutic target...
  47. ncbi Muscle- and fibre type-specific expression of glucose transporter 4, glycogen synthase and glycogen phosphorylase proteins in human skeletal muscle
    Jens R Daugaard
    Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    Pflugers Arch 447:452-6. 2004
    ..These findings imply that it is not possible to generalize fibre type-dependent protein expression on the basis of biopsies from only one muscle...
  48. ncbi Pyridoxal phosphate site in glycogen phosphorylase b: structure in native enzyme and in three derivatives with modified cofactors
    N G Oikonomakos
    Laboratory of Molecular Biophysics, University of Oxford, U K
    Biochemistry 26:8381-9. 1987
    ..abstract truncated at 250 words)..
  49. ncbi Allosteric effectors and trehalose protect larval Manduca sexta fat body glycogen phosphorylase B against thermal denaturation
    J R Meyer-Fernandes
    Department of Biochemistry and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, USA
    Insect Biochem Mol Biol 30:473-8. 2000
    ..Among the polyols tested, trehalose was the most effective in conferring thermal stability. In fact, in the presence of 20 mM AMP and 600 mM trehalose, 90% of the enzyme activity remained after 20 min at 60 degrees C...
  50. doi Disruption of the allosteric phosphorylase a regulation of the hepatic glycogen-targeted protein phosphatase 1 improves glucose tolerance in vivo
    Ian R Kelsall
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee, Scotland, UK
    Cell Signal 21:1123-34. 2009
    ....
  51. ncbi Enzymatic deimination of glycogen phosphorylase and a peptide of the phosphorylation site: identification of modification and roles in phosphorylation and activity
    S Luo
    Department of Biochemistry and Biophysics, Iowa State University, Ames 50011, USA
    Arch Biochem Biophys 318:362-9. 1995
    ..The results show the importance of the guanidino group of arginine-16 of the protein substrate in modulating the phosphorylase kinase reaction...
  52. ncbi Thermodynamic characterization of 5'-AMP binding to bovine liver glycogen phosphorylase a
    L García-Fuentes
    Departamento de Quimica Fisica, Bioquimica y Quimica Inorganica, Facultad de Ciencias Experimentales, Universidad de Almeria, Almeria, Spain
    J Biol Chem 271:27569-74. 1996
    ..45 +/- 0.02 kJ K-1 (mol of 5'-AMP bound)-1, which suggests significant changes in the polar and apolar surfaces accessible to the solvent...
  53. ncbi Site-directed mutants of glycogen phosphorylase are altered in their interaction with phosphorylase kinase
    A C Biorn
    Department of Biochemistry, Biophysics, and Molecular Biology, 4232 Molecular Biology Building, Iowa State University, Ames, Iowa 50011, USA mail med upenn edu
    Biochemistry 39:15887-94. 2000
    ..A specific interaction between Lys 11 of phosphorylase and Glu 110 of gamma(1-300) was also confirmed. In addition, I13G and R16A were able to be phosphorylated by protein kinase A, which does not recognize native phosphorylase...
  54. ncbi Glutathione-dependent reduction of arsenate by glycogen phosphorylase responsiveness to endogenous and xenobiotic inhibitors
    Zoltan Gregus
    Department of Pharmacology and Pharmacotherapy, Toxicology Section, University of Pecs, Medical School, Szigeti ut 12, H 7624 Pecs, Hungary
    Toxicol Sci 100:44-53. 2007
    ..It was also shown that a GPa-rich extract of rat liver contained GSH-dependent As(V)-reducing activity that was inhibited by specific GPIs, suggesting that the liver-type GPa can also catalyze reduction of As(V)...
  55. ncbi Physiological control of liver glycogen metabolism: lessons from novel glycogen phosphorylase inhibitors
    Loranne Agius
    Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle upon Tyne NE24HH, UK
    Mini Rev Med Chem 10:1175-87. 2010
    ....
  56. ncbi Amphibian glucagon family peptides: potent metabolic regulators in fish hepatocytes
    T P Mommsen
    Department of Biochemistry and Microbiology, University of Victoria, P O Box 3055, V8W 3P6, Victoria, BC, Canada
    Regul Pept 99:111-8. 2001
    ..We conclude that the broad physiological effectiveness of GLP-1 has been retained throughout vertebrate evolution, and that both insulinotropic activity and glycogenolytic actions belong to the repertoire of GLP-1...
  57. ncbi Purification and crystallization of glycogen phosphorylase from Saccharomyces cerevisiae
    V L Rath
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448
    J Mol Biol 225:1027-34. 1992
    ..1 A and c = 175.5 A Based on the density of the crystals, the solvent content is 49.7%, indicating that the asymmetric unit contains the functional dimer of yeast phosphorylase...
  58. ncbi Studies on the expression and evolution of the glycogen phosphorylase gene family in the rat
    M M Crerar
    Department of Biology, York University, North York, Ont, Canada
    Genome 30:582-90. 1988
    ..Differences in the relative rates of change of the three isozymes were observed and this may reflect different constraints on their evolution perhaps related to their functional roles and (or) tissue-specific expression...
  59. ncbi Genetic and radiation-reduced somatic cell hybrid sublocalization of the human GSTP1 gene
    C M Smith
    Department of Genetics, Washington University School of Medicine, St Louis MO 63110, USA
    Cytogenet Cell Genet 71:235-9. 1995
    ..74. Linkage analysis of the Venezuelan reference pedigree places GSTP1 5 cM distal to PYGM and 4 cM proximal to FGF3 thereby providing a genetic marker half-way between these two loci...
  60. ncbi Purification and properties of glycogen phosphorylase from the fat body of larval Manduca sexta
    E L Arrese
    Department of Biochemistry, University of Arizona, Tucson 85721
    Insect Biochem Mol Biol 25:209-16. 1995
    ..6-1.7-fold higher than the specific activity of the b form under the same conditions. Thus, 5'-AMP activates the a form by about 20%, whereas ATP has no effect on the phosphorylase a activity...
  61. pmc Regulation of human basophil function by phosphatase inhibitors
    M J Peirce
    Department of Medicine and Pharmacology, University of Sheffield, Royal Hallamshire Hospital Floor L
    Br J Pharmacol 119:446-53. 1996
    ..7. In total these data suggest that PPs are resident in human basophils and that PPs may be important in the regulation of basophil function...
  62. pmc Characterization of a gene cluster for glycogen biosynthesis and a heterotetrameric ADP-glucose pyrophosphorylase from Bacillus stearothermophilus
    H Takata
    Biochemical Research Laboratory, Ezaki Glico Co, Ltd, Nishiyodogawa ku, Osaka, Japan
    J Bacteriol 179:4689-98. 1997
    ..Glycogen metabolism in B. stearothermophilus is discussed on the basis of these results...
  63. ncbi Structural and functional characterization of the Drosophila glycogen phosphorylase gene
    G Tick
    Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, 6701, Hungary
    Biochem Biophys Res Commun 257:34-43. 1999
    ..All the residues of the allosteric effector binding sites, the active site, and the site of phosphorylation are exactly conserved, but some of the residues of the glycogen storage site are not...
  64. ncbi Effects of acute handling stress on whitefish Coregonus lavaretus after prolonged exposure to biologically treated and untreated bleached kraft mill effluent
    J Lappivaara
    Department of Biological and Environmental Science, University of Jyvaskyla, P O Box 35, FIN 40351 Jyvaskyla, Finland
    Arch Environ Contam Toxicol 41:55-64. 2001
    ..According to the present findings, the sensitivity of some widely used cellular and physiological variables may be improved by time-dependent standardization when interpreting data obtained following delayed sampling...
  65. ncbi Effects of arginine on pancreatic hormones and hepatic metabolism in rainbow trout
    T P Mommsen
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
    Physiol Biochem Zool 74:668-78. 2001
    ..Our data clearly show the dual role of Arg in fish metabolism...
  66. ncbi Three-dimensional structure of phosphorylase kinase at 22 A resolution and its complex with glycogen phosphorylase b
    Catherine Venien-Bryan
    Laboratory of Molecular Biophysics, Oxford Centre for Molecular Sciences, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Structure 10:33-41. 2002
    ..The PhK/GPb model provides an explanation for the formation of hybrid GPab intermediates in the PhK-catalyzed phosphorylation of GPb...
  67. pmc The effect of glucose on the potency of two distinct glycogen phosphorylase inhibitors
    Birgitte Andersen
    Department of Hepatic Biochemistry, Novo Nordisk A S, Novo Nordisk Park, DK 2760 Måløv, Denmark
    Biochem J 367:443-50. 2002
    ..In conclusion, the present study shows that the two GP inhibitors are kinetically very distinct and neither of the inhibitors demonstrates a physiologically relevant glucose dependence in vitro...
  68. ncbi Glycogen phosphorylase as a molecular target for type 2 diabetes therapy
    Nikos G Oikonomakos
    Institute of Biological Research and Biotechnology, The National Hellenic Research Foundation, 48 Vas Constantinou Avenue, Athens 11635, Greece
    Curr Protein Pept Sci 3:561-86. 2002
    ....
  69. ncbi [Analysis of 12 cases of McArdle's disease diagnosed after 30 years]
    M Pavic
    Service de Medecine Interne, Hopital d Instruction des Armees Desgenettes, 108, boulevard Pinel, 69998 Lyon Armées, France
    Rev Med Interne 24:716-20. 2003
    ..McArdle's disease (MAD) or glycogen storage disease type V, usually starts in childhood or adolescence. Generally diagnosis is made before the early adulthood because patients present well defined syndrome and are constrained...
  70. ncbi Cardioprotective effects of ingliforib, a novel glycogen phosphorylase inhibitor
    W Ross Tracey
    Pfizer Global Research and Development, MS8220 3125, Eastern Point Rd, Groton, CT 06340, USA
    Am J Physiol Heart Circ Physiol 286:H1177-84. 2004
    ....
  71. ncbi Effects of low-dose leptin and gender on the physiology of the mouse (Mus musculus)
    A M Mustonen
    Department of Biology, University of Joensuu, Finland
    J Anim Physiol Anim Nutr (Berl) 86:166-73. 2002
    ..Leptin seems to have minor but widespread effects on the energy metabolism of a nonmutant rodent. In nature, one function of leptin could be carbohydrate preservation...
  72. ncbi Dual-action hypoglycemic and hypocholesterolemic agents that inhibit glycogen phosphorylase and lanosterol demethylase
    H James Harwood
    Departments of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development, Groton Laboratories, Pfizer Inc, Eastern Point Road, Groton, CT 06340, USA
    J Lipid Res 46:547-63. 2005
    ..Dual-action glycogenolysis and cholesterolgenesis inhibitors therefore have the potential to favorably affect both the hyperglycemia and the dyslipidemia of type 2 diabetes...
  73. ncbi Role of beta-adrenoceptor signaling and AMP-activated protein kinase in glycolysis of postmortem skeletal muscle
    Min Du
    Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071, USA
    J Agric Food Chem 53:3235-9. 2005
    ..On the other hand, the activation of AMPK is important for maintaining the activity of glycogen phosphorylase and pyruvate kinase, leading to a sustained glycolysis and a low ultimate pH...
  74. doi Adenovirus and adeno-associated virus-mediated delivery of human myophosphorylase cDNA and LacZ cDNA to muscle in the ovine model of McArdle's disease: expression and re-expression of glycogen phosphorylase
    J McC Howell
    Department of Veterinary Biology and Biomedical Science, Murdoch University, Perth 6150, WA, Australia
    Neuromuscul Disord 18:248-58. 2008
    At present there is no satisfactory treatment for McArdle's disease, deficiency of myophosphorylase. Injection of modified adenovirus 5 (AdV5) and adeno-associated virus 2 (AAV2) vectors containing myophosphorylase expression cassettes, ..
  75. doi Shift in metabolic fuel in acylation-stimulating protein-deficient mice following a high-fat diet
    Christian Roy
    Centre de recherche Hopital Laval, Y 2186, Chemin Ste Foy, QC, Canada
    Am J Physiol Endocrinol Metab 294:E1051-9. 2008
    ..However, these in vivo data suggest that ASP inhibition could be a potentially viable approach in correcting muscle metabolic dysfunction in obesity...
  76. doi Structure-activity relationships of flavonoids as potential inhibitors of glycogen phosphorylase
    Atsushi Kato
    Department of Hospital Pharmacy, University of Toyama, Toyama 930 0194, Japan
    J Agric Food Chem 56:4469-73. 2008
    ..2 and 68.7 microM, respectively. These results suggested that as a group of novel GP inhibitors, flavonoids have potential to contribute to the protection or improvement of control of diabetes type II...
  77. doi Practical synthesis of bredemolic acid, a natural inhibitor of glycogen phosphorylase
    Keguang Cheng
    Center for Drug Discovery, College of Pharmacy, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing 210009, People s Republic of China
    J Nat Prod 71:1877-80. 2008
    ..Compounds 3 and 4 exhibited significant inhibitory activity against rabbit muscle GPa with IC(50) values of 6.25 and 1.1 μM, respectively...
  78. doi Anthranilimide-based glycogen phosphorylase inhibitors for the treatment of Type 2 diabetes: 2. Optimization of serine and threonine ether amino acid residues
    Steven M Sparks
    Metabolic Center for Excellence in Drug Discovery, GlaxoSmithKline, 5 Moore Drive, PO Box 13398, Research Triangle Park, NC 27705, USA
    Bioorg Med Chem Lett 19:981-5. 2009
    ..t-Butylthreonine analog 20 displayed potent in vitro inhibition of GPa, low potential for P450 inhibition, and excellent pharmacokinetic properties...
  79. doi Splice mutations preserve myophosphorylase activity that ameliorates the phenotype in McArdle disease
    John Vissing
    Department of Neurology 2082, University of Copenhagen, Rigshospitalet, Blegdamsvej 9, Copenhagen, Denmark
    Brain 132:1545-52. 2009
    Over 100 mutations in the myophosphorylase gene, which cause McArdle disease, are known...
  80. ncbi High-resolution melting facilitates mutation screening of PYGM in patients with McArdle disease
    Morten Duno
    Department of Clinical Genetics, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Ann Hum Genet 73:292-7. 2009
    Mutations in PYGM, encoding the muscle-specific glycogen phosphorylase (myophosphorylase), are responsible for McArdle disease...
  81. doi Investigation of a multimarker approach to the initial assessment of patients with acute chest pain
    C J McCann
    Royal Victoria Hospital, Belfast, Northern Ireland, UK
    Adv Ther 26:531-4. 2009
    ..Our research demonstrated that, in patients presenting within 4 hours from pain onset, H-FABP may improve detection of ACS. Measuring H-FABP and proBNP may help improve long-term risk stratification...
  82. doi Novel therapeutics based on inhibiting the interaction of glycogen phosphorylase and GL-subunit of glycogen-associated protein phosphatase 1: WO2009127723
    Lei Zhang
    Drug Discovery and Design Centre, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi Tech Park, Shanghai 201203, China
    Expert Opin Ther Pat 20:969-73. 2010
    ..Although lacking detailed in vivo biological data, WO2009127723 represents the first evidence for the novel rationale in antidiabetic pursuit, meriting the attention of the antidiabetic community...
  83. doi Organ-specific ATPase and phosphorylase enzyme activities in a food fish exposed to a carbamate insecticide and recovery response
    Ghousia Begum
    Fisheries Lab, Department of Zoology, College of Science, Osmania University, Hyderabad, AP, India
    Fish Physiol Biochem 37:61-9. 2011
    ..Recovery was not observed in phosphorylases. Thus, the results of the present study demonstrated the acute effect of carbofuran on a food fish and organ-specific recovery response to insecticidal treatment...
  84. ncbi Synthesis of N-glucopyranosidic derivatives as potential inhibitors that bind at the catalytic site of glycogen phosphorylase
    Thanasis Gimisis
    Organic Chemistry Laboratory, Department of Chemistry, School of Sciences, National and Kapodistrian University of Athens, 15771 Panepistimiopolis, Athens, Greece
    Mini Rev Med Chem 10:1127-38. 2010
    ....
  85. doi Small peptides released from muscle glycolytic enzymes during dry-cured ham processing
    L Mora
    Instituto de Agroquimica y Tecnologia de Alimentos CSIC, Avenida Agustín Escardino, Paterna, Valencia, Spain
    J Proteomics 74:442-50. 2011
    ....
  86. doi Involvement of nitric oxide in corticosterone release and glucose metabolism in food deprived rats
    B Mornagui
    Laboratoire de Physiologie des Agressions, Faculte des Sciences de Tunis, El Manar, Tunisia
    Horm Metab Res 43:369-73. 2011
    ..However, more studies are necessary to further clarify the mechanisms by which NO induces these responses...
  87. doi Does the crowded cell-like environment reduce the chaperone-like activity of α-crystallin?
    Svetlana G Roman
    Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr 33, Moscow 119071, Russia
    Biochemistry 50:10607-23. 2011
    ..However, crowding stimulates the rate of aggregation of these complexes, resulting in the above-mentioned decrease in the chaperone-like activity of α-crystallin...
  88. doi Binding evaluation of fragment-based scaffolds for probing allosteric enzymes
    Isabelle Krimm
    Institut des Sciences Analytiques, UMR CNRS 5280, Universite de Lyon, Universite Claude Bernard Lyon 1, Bât CPE Lyon, Domaine Scientifique de la Doua, F 69622 Villeurbanne, France
    J Med Chem 55:1287-95. 2012
    ..The work we report illustrates the power of fragmentlike ligands not only for probing the various binding pockets of proteins, but also for uncovering cooperativity between these various binding sites...
  89. doi Synthesis of heterocyclic N-(β-D-glucopyranosyl)carboxamides for inhibition of glycogen phosphorylase
    Bálint Kónya
    Department of Organic Chemistry, University of Debrecen, PO Box 20, H 4010 Debrecen, Hungary
    Carbohydr Res 351:56-63. 2012
    ..The best inhibitors of the two series were N-(β-D-glucopyranosyl)-1-(3,5-dimethyl-phenyl)-1,2,3-triazole-4-carboxamide (K(i)=34 μM) and N-(β-D-glucopyranosyl)-3-(indol-2-yl)-isoxazole-5-carboxamide (K(i)=164 μM)...
  90. doi Modulatory effect of green tea extract on hepatic key enzymes of glucose metabolism in streptozotocin and high fat diet induced diabetic rats
    Ramalingam Sundaram
    Department of Medical Biochemistry, Dr ALM P G, Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, Tamil Nadu, India
    Phytomedicine 20:577-84. 2013
    ..Thus, this study indicates that the administration of green tea extract to diabetic rats resulted in alterations in the metabolism of glucose with subsequent reduction in plasma glucose levels...
  91. ncbi [McArdle disease presenting with rhabdomyolisis and acute kidney injury]
    Rui Costa
    Serviço de Nefrologia Centro Hospitalar de Trás os Montes e Alto Douro Vila Real Portugal
    Acta Med Port 26:463-6. 2013
    ..Deficiency of myophosphorylase enzyme results in inability to degrade glycogen stores, causing glycogen accumulation in muscle tissue and ..
  92. pmc Body morphology, energy stores, and muscle enzyme activity explain cricket acoustic mate attraction signaling variation
    Ian R Thomson
    Department of Biology, Carleton University, Ottawa, Ontario, Canada
    PLoS ONE 9:e90409. 2014
    ..Our findings indicate that a physiological perspective may help us to understand some of the causes of variation in behaviour. ..
  93. doi Nymphayol increases glucose-stimulated insulin secretion by RIN-5F cells and GLUT4-mediated insulin sensitization in type 2 diabetic rat liver
    P Subash-Babu
    Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, P O Box 2460, Riyadh 11451, Saudi Arabia
    Chem Biol Interact 226:72-81. 2015
    ..05) reduction in blood glucose in STZ-nicotinamide induced diabetic rats. The results suggest that nymphayol may be a useful therapy for diabetes because it stimulates insulin secretion and promotes glucose absorption...
  94. doi Lowering Temperature is the Trigger for Glycogen Build-Up and Winter Fasting in Crucian Carp (Carassius carassius)
    Joonas Varis
    Department of Biology, University of Eastern Finland, Yliopistokatu 7, 80101 Joensuu, Natura building, Finland
    Zoolog Sci 33:83-91. 2016
    ..Shortened day-length and oxygen availability had practically no effects on measured variables. We conclude that lowering temperature is the main trigger in preparation for winter anoxia in crucian carp. ..
  95. doi Russelioside B, a pregnane glycoside ameliorates hyperglycemia in streptozotocin induced diabetic rats by regulating key enzymes of glucose metabolism
    Essam Abdel-Sattar
    Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt Electronic address
    Chem Biol Interact 252:47-53. 2016
    ..Hence, administration of russelioside B may represent a potentially useful strategy for the management of diabetes. ..
  96. ncbi Glycogen serves as an energy source that maintains astrocyte cell proliferation in the neonatal telencephalon
    Hitoshi Gotoh
    Department of Biology, Kyoto Prefectural University of Medicine, Sakyo ku, Kyoto, Japan
    J Cereb Blood Flow Metab . 2016
    ..These results suggest that glycogen accumulates in astrocytes located in specific areas during the prenatal stage and is used as an energy source to maintain normal development in the early postnatal stage...
  97. doi Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies
    Ana Mrkaić
    a Mountainview Hospital, Las Vegas, NV, USA
    J Matern Fetal Neonatal Med . 2017
    ....
  98. doi Discovery of new nanomolar inhibitors of GPa: Extension of 2-oxo-1,2-dihydropyridinyl-3-yl amide-based GPa inhibitors
    Wendy A Loughlin
    School of Natural Sciences, Griffith University, Brisbane, QLD, 4111, Australia Electronic address
    Eur J Med Chem 127:341-356. 2016
    ..Upon exploring a dimer strategy as part of the SAR analysis, the first extended 2-oxo-dihydropyridinyl-3-yl amide nanomolar based inhibitors of GPa (IC50 = 230 and 260 nM) were identified...
  99. ncbi Glycogenosis type V (McArdle's disease) mimicking atypical myositis
    G Horneff
    Department of Paediatrics, University Hospital, Martin Luther University Halle Wittenberg, Germany
    Clin Rheumatol 20:57-60. 2001
    ..b>Myophosphorylase activity was completely lacking, establishing the diagnosis of McArdle's disease...
  100. ncbi Daily variation in the distribution of glycogen phosphorylase in the suprachiasmatic nucleus of Syrian hamsters
    C W Harley
    Department of Psychology, Memorial University of Newfoundland, St John s, Newfoundland A1B 3X9, Canada
    J Comp Neurol 435:249-58. 2001
    ..Increased GP activity also occurs in astrocytes among optic fibers subjacent to the SCN during light input...
  101. ncbi Two novel mutations in the myophosphorylase gene in a patient with McArdle disease
    Marcus Deschauer
    Department of Neurology, Martin Luther Universitat Halle Wittenberg, Ernst Grube Str 40, 06097 Halle Saale, Germany
    Muscle Nerve 27:105-7. 2003
    We identified two novel mutations in exon 2 of the myophosphorylase gene in a 33-year-old German women with McArdle disease...

Research Grants30

  1. EVOLUTION OF ALLOSTERIC REGULATION IN PHOSPHORYLASE
    Robert Fletterick; Fiscal Year: 1999
    ..These basic mechanisms have strong eventual implications for developing therapy to help persons with diabetes and other metabolic disorders, such as hepatic glycogen storage diseases. ..
  2. SUBUNIT INTERACTIONS OF PHOSPHORYLASE KINASE
    Gerald M Carlson; Fiscal Year: 2013
    ....
  3. Gerald M Carlson; Fiscal Year: 2014
    ....
  4. ENZYMIC AND NON-ENZYMIC GLYCOSYL TRANSFER
    Michael Sinnott; Fiscal Year: 1993
    ....
  5. STRUCTURE-FUNCTION RELATIONSHIPS IN PHOSPHORYLASE A
    Robert Fletterick; Fiscal Year: 1993
    ..The structure role of the covalent phosphorylation of these enzymes and its relationship role to the allosteric regulation by small molecule ligands will be determined...
  6. HORMONAL REGULATION OF PHOSPHOINOSITIDE METABOLISM
    JOHN FAIN; Fiscal Year: 1991
    ..This project is primarily a metabolic investigation using biochemical techniques. The studies are done in experimental animals and should provide significant insights into various diseases of metabolism and especially diabetes...
  7. CONTROL OF PEPTIDE HORMONE LEVELS IN INSECTS
    Rolf Ziegler; Fiscal Year: 1991
    ..Using antibodies, the cDNA and the gene coding for AKH will be isolated and will be characterized. The structure of the gene will help us to understand the synthesis of AKH...
  8. GLUCOCORTICOID PERMISSIVE ACTION ON LIVER METABOLISM
    Timothy Chan; Fiscal Year: 1980
    ..The effects of adrenalectomy on glucagon action on phosphofructokinase and mitochondrial pyruvate metabolism will be examined in the context of the lack of glucagon stimulation of gluconeogenesis. ..
  9. GUANIDINE SPECIFIC MONO ADP-RIBOSYLATION REACTIONS
    Donald Graves; Fiscal Year: 1990
    ..Kinetic studies B. Chemical characterization III. Chemical aspects of mono ADP-ribosylation and factors influencing the specificity of the enzymatic reactions A. Specificity B. Affinity HPLC C. Active site reagents - ADP-ribosylation..
  10. CONTROL OF ENZYME BIOSYNTHESIS--ROLE OF CYCLIC AMP
    HOWARD RICKENBERG; Fiscal Year: 1980
    ..In unrelated experiments with Escherichia coli the effect of the cyclic AMP-CRP complex on the synthesis of ribosomal proteins will be studied...
  11. FUNCTIONAL METABOLIC ACTIVITY IN GLIA IN VIVO
    Gerald Dienel; Fiscal Year: 2003
    ..abstract_text> ..
  12. FUNCTIONAL METABOLISM IN WORKING BRAIN
    Gerald Dienel; Fiscal Year: 2007
    ..abstract_text> ..
  13. ADAPTATION OF SENESCENT MUSCLE TO EXERCISE TRAINING
    Todd Trappe; Fiscal Year: 2002
    ....
  14. Analgesics and resistance training in older individuals
    Todd Trappe; Fiscal Year: 2008
    ..This investigation will provide important information for clinicians and older individuals with regard to expected benefits from physical activity during periods of analgesic and anti-inflammatory based therapy. ..
  15. Molecular Mechanims of the Menin Tumor Suppressor Gene
    Mark Sawicki; Fiscal Year: 2004
    ..2. Characterize the function of menin as a DDKI (Dbf4 dependent kinase inhibitor). 3. Identify physiological factors and their underlying molecular mechanisms important for modulating menin DDKI function...
  16. METABOLIC CONTROL AND MOLECULAR ADAPTATION IN DROSOPHILA
    WALTER EANES; Fiscal Year: 2005
    ..Studies will begin to identify the genes responsible for the variation in this suite of traits that have cascading effects into the metabolic pathways. ..
  17. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2006
    ..abstract_text> ..
  18. Myosin-based kinetic differences between muscle types
    David Maughan; Fiscal Year: 2006
    ..abstract_text> ..
  19. DO LIGHT CHAIN EXTENSIONS ENHANCE MUSCLE POWER OUTPUT?
    David Maughan; Fiscal Year: 2004
    ..This research will contribute to understanding and treating human muscle diseases in which power output is compromised. ..
  20. MECHANISMS OF NO-MEDIATED PROTEIN S-NITROSYLATION IN EAE
    OSCAR BIZZOZERO; Fiscal Year: 2007
    ..abstract_text> ..
  21. CLINICAL RESEARCH CENTER FOR NEUROMUSCULAR DISEASE
    Salvatore DiMauro; Fiscal Year: 2007
    ....
  22. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2007
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  23. MOLECULAR BASIS OF DIABETIC CARDIOMYOPATHY
    David Maughan; Fiscal Year: 2003
    ..This research will contribute to understanding and treating human myocardial pathologies that arise from diabetes mellitus, with particular attention to diastolic dysfunctions. (End of Abstract) ..
  24. Triple threat screening for modifiers of Protein Ser/Thr Phosphatase 2C
    DAVID BRAUTIGAN; Fiscal Year: 2008
    ..Chemical modifiers of PP2C have potential medicinal applications and will be used as research tools to study signaling in normal and cancer cells. [unreadable] [unreadable] [unreadable]..
  25. NMR STUDIES:HEPATIC GLUCOSE METABOL IN HUMANS EXERCISING
    Kitt Petersen; Fiscal Year: 2003
    ..The results of these studies should provide important new insight into the regulation of hepatic glucose metabolism in healthy and type 1 diabetic subjects. ..
  26. NITRIC OXIDE, PLP ACYLATION & THE PATHOPHYSIOLOGY OF MS
    OSCAR BIZZOZERO; Fiscal Year: 2003
    ..abstract_text> ..