PSMD4

Summary

Gene Symbol: PSMD4
Description: proteasome 26S subunit, non-ATPase 4
Alias: AF-1, ASF, MCB1, Rpn10, S5A, pUB-R5, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit S5A, S5a/antisecretory factor protein, angiocidin, antisecretory factor 1, multiubiquitin chain-binding protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Species: human

Top Publications

  1. pmc Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation
    M Lamar Seibenhener
    Program in Cell and Molecular Biosciences, Department of Biological Sciences, Auburn University, AL 36849, USA
    Mol Cell Biol 24:8055-68. 2004
  2. pmc Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP
    K Matthew Scaglione
    Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 43:599-612. 2011
  3. pmc Solution structure of the E3 ligase HOIL-1 Ubl domain
    Steven A Beasley
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Protein Sci 21:1085-92. 2012
  4. ncbi The effects of the polyglutamine repeat protein ataxin-1 on the UbL-UBA protein A1Up
    Brigit E Riley
    Department of Biochemistry, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:42290-301. 2004
  5. pmc The UBL domain of PLIC-1 regulates aggresome formation
    Renu Heir
    Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Montreal, Quebec H3A2B2, Canada
    EMBO Rep 7:1252-8. 2006
  6. pmc HIV-1 replication through hHR23A-mediated interaction of Vpr with 26S proteasome
    Ge Li
    Department of Pathology, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 5:e11371. 2010
  7. ncbi Characterization of two polyubiquitin binding sites in the 26 S protease subunit 5a
    P Young
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    J Biol Chem 273:5461-7. 1998
  8. ncbi Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome
    H Hiyama
    Institute for Molecular and Cellular Biology, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 274:28019-25. 1999
  9. pmc Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes
    H Kawahara
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    EMBO J 19:4144-53. 2000
  10. ncbi Targeting of NEDD8 and its conjugates for proteasomal degradation by NUB1
    T Kamitani
    Department of Cardiology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 276:46655-60. 2001

Research Grants

  1. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
  2. ALBERT HONG JAE KIM; Fiscal Year: 2016
  3. Molecular Mechanisms that Cause Muscle Atrophy
    Alfred L Goldberg; Fiscal Year: 2012
  4. Steven R Grossman; Fiscal Year: 2014
  5. SYSTEMATIC ANALYSIS OF PROTEOLYSIS PATHWAYS FOR CULLIN TARGETS
    JEFFREY WADE HARPER; Fiscal Year: 2012
  6. NMR Structural Studies of Ubiquitin Receptor Protein Complexes
    Kylie J Walters; Fiscal Year: 2013
  7. Ilker Kudret Sariyer; Fiscal Year: 2016
  8. VAISHALI MOULTON; Fiscal Year: 2016
  9. ASF/SF2: Phosphorylation and RNA Binding
    Gourisankar Ghosh; Fiscal Year: 2012
  10. Regulation of tumorigenic spliced isoforms in cancer
    Aixa Tapia Santos; Fiscal Year: 2010

Detail Information

Publications212 found, 100 shown here

  1. pmc Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation
    M Lamar Seibenhener
    Program in Cell and Molecular Biosciences, Department of Biological Sciences, Auburn University, AL 36849, USA
    Mol Cell Biol 24:8055-68. 2004
    ..Altogether, our results support the hypothesis that p62 may act as a critical ubiquitin chain-targeting factor that shuttles substrates for proteasomal degradation...
  2. pmc Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP
    K Matthew Scaglione
    Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 43:599-612. 2011
    ..In addition, the results shed light on disease pathogenesis in SCA3, a neurodegenerative disorder caused by polyglutamine expansion in ataxin-3...
  3. pmc Solution structure of the E3 ligase HOIL-1 Ubl domain
    Steven A Beasley
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Protein Sci 21:1085-92. 2012
    ..Further, the Ubl domain of both HOIL-1 and parkin has been shown to interact with the S5a subunit of the 26S proteasome...
  4. ncbi The effects of the polyglutamine repeat protein ataxin-1 on the UbL-UBA protein A1Up
    Brigit E Riley
    Department of Biochemistry, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:42290-301. 2004
    ..Similar to other UbL-containing proteins, the UbL of A1Up is essential for the interaction of A1Up with the S5a subunit of the 19S proteasome...
  5. pmc The UBL domain of PLIC-1 regulates aggresome formation
    Renu Heir
    Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Montreal, Quebec H3A2B2, Canada
    EMBO Rep 7:1252-8. 2006
    ..is a ubiquitin-like protein that binds to the ubiquitin-interacting motif (UIM) of the proteasomal subunit S5a. Here, we show that PLIC-1 also binds to the UIM proteins ataxin 3--a deubiquitinating enzyme--HSJ1a--a co-..
  6. pmc HIV-1 replication through hHR23A-mediated interaction of Vpr with 26S proteasome
    Ge Li
    Department of Pathology, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 5:e11371. 2010
    ..further confirmed in mammalian cells where Vpr associates with the mammalian orthologues of fission yeast Mts4 and S5a. Consistently, depletion of hHR23A interrupts interaction of Vpr with proteasome in mammalian cells...
  7. ncbi Characterization of two polyubiquitin binding sites in the 26 S protease subunit 5a
    P Young
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    J Biol Chem 273:5461-7. 1998
    ..One of these proteins, subunit 5a (S5a), has been shown to bind ubiquitin-lysozyme conjugates and free polyubiquitin chains...
  8. ncbi Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome
    H Hiyama
    Institute for Molecular and Cellular Biology, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 274:28019-25. 1999
    ..that the human NER factor hHR23B as well as another human homolog of RAD23, hHR23A, interact specifically with S5a, a subunit of the human 26 S proteasome using the yeast two-hybrid system...
  9. pmc Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes
    H Kawahara
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    EMBO J 19:4144-53. 2000
    ..To date only a single species of Rpn10, which possibly functions as a multiubiquitin chain-binding subunit, has been identified in various organisms...
  10. ncbi Targeting of NEDD8 and its conjugates for proteasomal degradation by NUB1
    T Kamitani
    Department of Cardiology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 276:46655-60. 2001
    ..276, 20603-20609). Here, we show that NUB1 possesses a ubiquitin-like domain at the N-terminal region and binds to S5a of the 19 S proteasome activator (PA700)...
  11. ncbi Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a
    Kylie J Walters
    Department of Pathology and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 41:1767-77. 2002
    ..We have focused on how the S5a component of the 19S complex interacts with different ubiquitin-like (ubl) modules, to advance our understanding of ..
  12. pmc Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain
    Eri Sakata
    Department of Structural Biology and Biomolecular Engineering, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya 467 8603, Japan
    EMBO Rep 4:301-6. 2003
    ..Inspection of chemical-shift-perturbation data showed that the parkin Ubl domain binds the Rpn10 subunit of 26S proteasomes via the region of parkin that includes position 42...
  13. pmc Structural determinants for the binding of ubiquitin-like domains to the proteasome
    Thomas D Mueller
    Department of Chemistry and Biochemistry, 405 Hilgard Avenue, PO Box 951569, University of California, Los Angeles, CA 90095 1569, USA
    EMBO J 22:4634-45. 2003
    ..The Ubl domain binds with high affinity to the second ubiquitin-interacting motif (UIM) of the S5a subunit of the proteasome...
  14. ncbi Diverse polyubiquitin interaction properties of ubiquitin-associated domains
    Shahri Raasi
    Department of Biochemistry and Molecular Biology, Johns Hopkins School of Public Health, Baltimore, Maryland 21205, USA
    Nat Struct Mol Biol 12:708-14. 2005
    ..Conversely, non-UBA sequences can modulate the interaction properties of a UBA domain...
  15. ncbi Interaction of NUB1 with the proteasome subunit S5a
    Kunikazu Tanji
    Department of Cardiology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Biochem Biophys Res Commun 337:116-20. 2005
    ..Since NUB1 possesses a UBL domain, which was previously shown to be an S5a-interacting motif in RAD23/HHR23, we initially hypothesized that NUB1 interacts with the S5a subunit of the ..
  16. ncbi Adrm1, a putative cell adhesion regulating protein, is a novel proteasome-associated factor
    Jakob Ploug Jørgensen
    Institute of Molecular Biology and Physiology, University of Copenhagen, Universitetsparken 13, DK 2100 Copenhagen Ø, Denmark
    J Mol Biol 360:1043-52. 2006
    ..This indicates that Adrm1 has a specialised role in proteasome function...
  17. pmc A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    EMBO J 25:4524-36. 2006
    ..Knockdown of hRpn13 caused no obvious proteolytic defect but loss of UCH37 proteins and decrease in deubiquitinating activity of 26S proteasomes. Our results indicate that hRpn13 is essential for the activity of UCH37...
  18. pmc hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37
    Xiao Bo Qiu
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, 5 Dongdan Santiao, Beijing, China
    EMBO J 25:5742-53. 2006
    ..Thus in human 26S proteasomes, hRpn13 appears to be important for the binding of UCH37 to the 19S complex and for efficient proteolysis...
  19. pmc Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Mol Cell Biol 27:6629-38. 2007
    b>Rpn10 is a subunit of the 26S proteasome that recognizes polyubiquitinated proteins. The importance of Rpn10 in ubiquitin-mediated proteolysis is debatable, since a deficiency of Rpn10 causes different phenotypes in different organisms...
  20. pmc The ubiquitin-interacting motif protein, S5a, is ubiquitinated by all types of ubiquitin ligases by a mechanism different from typical substrate recognition
    Tomoaki Uchiki
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 284:12622-32. 2009
    S5a/Rpn10 is a ubiquitin (Ub)-binding protein that is a subunit of the 26S proteasome but also exists free in the cytosol. It binds poly-Ub chains through its two Ub-interacting motifs (UIMs)...
  21. pmc Thioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome
    Katrine M Andersen
    Department of Biology, University of Copenhagen, Universitetsparken 13, DK 2100 Copenhagen, Denmark
    J Biol Chem 284:15246-54. 2009
    ..Hence, Txnl1 is the first example of a direct connection between protein reduction and proteolysis, two major intracellular protein quality control mechanisms...
  22. doi Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones
    Takeumi Kaneko
    Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Cell 137:914-25. 2009
    ..These chaperones dissociate before completion of 26S proteasome formation. Our results demonstrate that base assembly is facilitated by multiple proteasome-dedicated chaperones, like CP assembly...
  23. pmc Differential interaction of the E3 ligase parkin with the proteasomal subunit S5a and the endocytic protein Eps15
    Susan S Safadi
    Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 285:1424-34. 2010
    ..The N-terminal ubiquitin-like domain (Ubld) of parkin functions with the S5a proteasomal subunit, positioning substrate proteins for degradation...
  24. doi Cross-species divergence of the major recognition pathways of ubiquitylated substrates for ubiquitin/26S proteasome-mediated proteolysis
    Antony S Fatimababy
    Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
    FEBS J 277:796-816. 2010
    ..of ubiquitin/26S proteasome-mediated proteolysis (UPP), which is mediated directly by the proteasome subunit RPN10 and/or RPN13, or indirectly by ubiquitin receptors containing ubiquitin-like and ubiquitin-associated domains...
  25. doi Functional differences between two major ubiquitin receptors in the proteasome; S5a and hRpn13
    Muthukumar Elangovan
    School of Life Sciences, Gwangju Institute of Science and Technology, 1 Oryong dong Buk ku, Gwangju 500 712, Republic of Korea
    Biochem Biophys Res Commun 396:425-8. 2010
    It is well known that S5a and hRpn13 are two major ubiquitin (Ub) receptors in the proteasome but little is known about their functional difference in recruiting ubiquitinated substrates...
  26. pmc Mdm2 facilitates the association of p53 with the proteasome
    Roman Kulikov
    Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, P O Box 3640, 76021 Karlsruhe, Germany
    Proc Natl Acad Sci U S A 107:10038-43. 2010
    ..We propose that Mdm2 facilitates, or at least enhances, the association of p53 with the proteasome and that phosphorylation of the central domain of Mdm2 regulates this process...
  27. pmc Monoubiquitination of RPN10 regulates substrate recruitment to the proteasome
    Marta Isasa
    Institut de Biologia Molecular de Barcelona, CSIC, Barcelona Scientific Park, Baldiri i Reixac 15 21, 08028 Barcelona, Spain
    Mol Cell 38:733-45. 2010
    The proteasome recognizes its substrates via a diverse set of ubiquitin receptors, including subunits Rpn10/S5a and Rpn13...
  28. doi Parkin directly modulates 26S proteasome activity
    Ji Won Um
    Department of Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea
    J Neurosci 30:11805-14. 2010
    ..The current findings point to a novel role for parkin for 26S proteasome assembly and suggest that parkin mutations contribute to the proteasomal dysfunction in PD...
  29. pmc Pharmacogenomics of bortezomib test-dosing identifies hyperexpression of proteasome genes, especially PSMD4, as novel high-risk feature in myeloma treated with Total Therapy 3
    John D Shaughnessy
    Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, Little Rock AR, USA
    Blood 118:3512-24. 2011
    ..PMSD4 was 1 of 3 genes common to both models. Residing on chromosome 1q21, PSMD4 expression is highly sensitive to copy number...
  30. doi FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis
    Neha Rani
    Division of Immunology, Department of Biology, University of Constance, 78457 Konstanz, Germany
    Nat Commun 3:749. 2012
    ..We identify the 26S proteasome subunit hRpn10/S5a as the receptor for FAT10, whereas NUB1L can bind to both Rpn10 and Rpn1/S2. Unexpectedly, FAT10 and NUB1L both interact with hRpn10 via the VWA domain...
  31. pmc Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes
    Gustavo Martinez-Noel
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA
    Mol Cell Biol 32:3095-106. 2012
    ..These proteomic studies reveal a level of complexity for E6AP that has not been previously appreciated and identify a number of new cellular proteins through which E6AP may be regulated or functioning...
  32. pmc Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation
    Jonas Boehringer
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Biochem J 448:55-65. 2012
    ..essential component of the 19S regulatory particle and plays a role in recruiting the extrinsic ubiquitin receptor Rpn10. In the present paper we report the crystal structure of Rpn12, a proteasomal PCI-domain-containing protein...
  33. pmc The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a
    A Sparks
    Division of Cancer Research, Medical Research Institute, Jacqui Wood Cancer Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK
    Oncogene 33:4685-96. 2014
    ..In this study, we show that knockdown of the proteasomal ubiquitin receptor S5a/PSMD4/Rpn10 inhibits p53 protein degradation and results in the accumulation of ubiquitinated p53...
  34. pmc Inherent asymmetry in the 26S proteasome is defined by the ubiquitin receptor RPN13
    Dikla Berko
    From the Department of Biochemistry, The Rappaport Family Institute for Research in the Medical Sciences, Technion Israel Institute of Technology, Haifa 31096, Israel
    J Biol Chem 289:5609-18. 2014
    ..majority (if not all) of the double-capped 26S proteasomes, both 19S complexes, contain the ubiquitin receptor Rpn10/S5a, only one of these 19S particles contains the additional ubiquitin receptor Rpn13, thereby defining asymmetry ..
  35. pmc Autoregulation of the 26S proteasome by in situ ubiquitination
    Andrew D Jacobson
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045
    Mol Biol Cell 25:1824-35. 2014
    ..Here we report that the human 26S proteasome is ubiquitinated, by which the ubiquitin receptors Adrm1 and S5a, the ATPase subunit Rpt5, and the deubiquitinating enzyme Uch37 are ubiquitinated in situ by proteasome-associating ..
  36. pmc S5a promotes protein degradation by blocking synthesis of nondegradable forked ubiquitin chains
    Hyoung Tae Kim
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 28:1867-77. 2009
    ..b>S5a is a ubiquitin interacting motif (UIM) protein present in the cytosol and in the 26S proteasome...
  37. ncbi Cloning and characterization of angiocidin, a tumor cell binding protein for thrombospondin-1
    Jing Zhou
    Temple University, Center for Neurovirology and Cancer Biology, 354 Biology Life Sciences Building 015 96, 1900 North 12th Street, Philadelphia, Pennsylvania 19122, USA
    J Cell Biochem 92:125-46. 2004
    ..The purified recombinant protein, termed angiocidin, is a potent inhibitor of tumor growth of Lewis Lung carcinoma in vivo and tumor invasion and angiogenesis in ..
  38. ncbi A regulated interaction with the UIM protein Eps15 implicates parkin in EGF receptor trafficking and PI(3)K-Akt signalling
    Lara Fallon
    Centre for Neuronal Survival and Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada H3A 2B4
    Nat Cell Biol 8:834-42. 2006
    ..Considering the role of Akt in neuronal survival, our results have broad new implications for understanding the pathogenesis of Parkinson's disease...
  39. pmc An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine
    T Lewis
    Plum Island Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Greenport, New York 11944 0848, USA
    J Virol 74:1275-85. 2000
    ..All pigs previously infected with Delta9GL survived infection when subsequently challenged with a lethal dose of virulent parental MAL. Thus, ASFV 9GL gene deletion mutants may prove useful as live-attenuated ASF vaccines.
  40. ncbi [Influence of angiotensin on the secretion of aldosterone in idiopathic hyperaldosteronism]
    M Salvador
    , , Toulouse
    Arch Mal Coeur Vaiss 80:992-4. 1987
    ..an increase of adrenal sensitivity to angiotensin II infusions, and isolated an aldosterone-stimulating factor (ASF). Plasma aldosterone levels were related to increased ASF concentrations but there was no link between PRA and ASF...
  41. ncbi Prospective pulmonary function evaluation following open thoracotomy for anterior spinal fusion in adolescent idiopathic scoliosis
    E J Graham
    Washington University, Department of Orthopaedic Surgery, St Louis, MO, USA
    Spine (Phila Pa 1976) 25:2319-25. 2000
    ..sequential pulmonary function tests (PFTs) at a minimum 2-year follow-up after an open anterior spinal fusion (ASF) with instrumentation for thoracic AIS...
  42. doi Assessment of interactions between African swine fever virus, bushpigs (Potamochoerus larvatus), Ornithodoros ticks and domestic pigs in north-western Madagascar
    J Ravaomanana
    FOFIFA, Malagasy Centre for Research in Agronomy, Antananarivo, Madagascar
    Transbound Emerg Dis 58:247-54. 2011
    Since its introduction in Madagascar in 1998, African swine fever (ASF) has severely affected national pig production and persists as a common disease in that country...
  43. doi Design and verification of a highly reliable Linear-After-The-Exponential PCR (LATE-PCR) assay for the detection of African swine fever virus
    B Ronish
    Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    J Virol Methods 172:8-15. 2011
    ..African swine fever virus (ASFV) is a highly pathogenic DNA virus that is the causative agent of African swine fever (ASF), an infectious disease of domestic and wild pigs of all breeds and ages, causing a range of syndromes...
  44. ncbi Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5
    T Maeda
    Department of Radiology and Radiation Oncology, Osaka University School of Dentistry, 1 8 Yamadaoka, Suita, Osaka 565 0871, Japan
    Carcinogenesis 20:2341-4. 1999
    ..Gene expression of three splicing regulatory factors, alternative splicing factor/splicing factor 2 (ASF/SF2), heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) and the 65 kDa subunit of U2 small nuclear ..
  45. ncbi Apical uptake of organic cations by human intestinal Caco-2 cells: putative involvement of ASF transporters
    F Martel
    Department of Biochemistry, Faculty of Medicine, Porto, Portugal
    Naunyn Schmiedebergs Arch Pharmacol 363:40-9. 2001
    ..organic cations, by testing the possibility of involvement of known members of the amphiphilic solute facilitator (ASF) family in this process...
  46. ncbi Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality. A controlled clinical study
    N B Arweiler
    Department of Periodontology and Conservative Dentistry, University of the Saarland, Homburg, Germany
    J Clin Periodontol 28:168-74. 2001
    ..of this study was to evaluate the clinical and antibacterial properties of alcohol-free mouthrinses, an amine fluoride/stannous fluoride mouthrinse (ASF) and two triclosan solutions in comparison with a chlorhexidine and a placebo rinse.
  47. pmc An RNA splicing enhancer-like sequence is a component of a splicing inhibitor element from Rous sarcoma virus
    L M McNally
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Mol Cell Biol 18:3103-11. 1998
    ..The second is a purine-rich region in the 5' half that interacts with the splicing factor SF2/ASF. In this study we investigated the possibility that this purine-rich region provides an RNA splicing enhancer ..
  48. pmc Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production
    Sandrine Jacquenet
    Laboratoire de Médecine et Thérapeutique Moléculaire, INSERM CIC9501, 15 rue du Bois de la Champelle, 54500 Vandoeuvre les Nancy, France
    Retrovirology 2:33. 2005
    ..and virion production, we used a human cell line expressing high levels of complete HIV-1 and either one of the ASF/SF2, SC35, and 9G8 SR proteins...
  49. pmc Ubiquitin docking at the proteasome through a novel pleckstrin-homology domain interaction
    Patrick Schreiner
    Center for Integrated Protein Science at the Department Chemie, Lehrstuhl für Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    Nature 453:548-52. 2008
    ..proteasome recognizes its substrates, as the only established ubiquitin receptor intrinsic to the proteasome is Rpn10/S5a (ref. 1), which is not essential for ubiquitin-mediated protein degradation in budding yeast...
  50. ncbi Serum concentrations of C-reactive protein, serum amyloid A, and haptoglobin in pigs inoculated with African swine fever or classical swine fever viruses
    Pedro J Sánchez-Cordón
    Departamento de Anatomia y Anatomia Patologica Comparadas, Facultad de Veterinaria, Universidad de Cordoba, Edificio de Sanidad Animal, Campus de Rabanales, 14014, Cordoba, Spain
    Am J Vet Res 68:772-7. 2007
    ..serum concentrations of the selected acute-phase proteins (APPs) haptoglobin, serum amyloid A (SAA), and C-reactive protein (CRP) in pigs experimentally inoculated with classical swine fever (CSF) and African swine fever (ASF) viruses.
  51. pmc The persistence of African swine fever virus in field-infected Ornithodoros erraticus during the ASF endemic period in Portugal
    Fernando S Boinas
    Centro de Investigação Interdisciplinar em Medicina Veterinária, Faculdade de Medicina Veterinária da Universidade Técnica de Lisboa, Lisboa, Portugal
    PLoS ONE 6:e20383. 2011
    African swine fever (ASF) is an important disease of pigs and outbreaks of ASF have occurred in Europe on multiple occasions...
  52. pmc Mapping the interactions between Lys48 and Lys63-linked di-ubiquitins and a ubiquitin-interacting motif of S5a
    Aydin Haririnia
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742, USA
    J Mol Biol 368:753-66. 2007
    ..Here we use NMR to study the interaction of a ubiquitin-interacting motif (UIM) of the proteasomal subunit S5a with di-ubiquitin, the simplest model for polyubiquitin chain, to gain insights into the mechanism of polyubiquitin ..
  53. pmc Gait in thoracolumbar/lumbar adolescent idiopathic scoliosis: effect of surgery on gait mechanisms
    Philippe Mahaudens
    Rehabilitation and Physical Medicine Unit, Universite Catholique de Louvain, Unite de Readaptation, Tour Pasteur 5375, Avenue Mounier 53, 1200, Brussels, Belgium
    Eur Spine J 19:1179-88. 2010
    ..The use of anterior spinal fusion (ASF) instead of the classic posterior fusion (PSF) may preserve more distal spinal levels in attempt to limit the ..
  54. ncbi Glutathione reduces the toxicity associated with antitumor therapy of ascites fluid adsorbed over Staphylococcus aureus Cowan I in tumor bearing mice
    A S Verma
    Industrial Toxicology Research Centre, Lucknow, India
    Toxicol Lett 106:119-27. 1999
    ..p.) on alternate days for 15 days with adsorbed ascites fluid (ad-ASF) (0.1 ml) and glutathione (GSH) (250 mg/kg body weight) separately...
  55. ncbi Visual, long-latency auditory and brainstem auditory evoked potentials in migraine: relation to pattern size, stimulus intensity, sound and light discomfort thresholds and pre-attack state
    T Sand
    Department of Clinical Neurosciences, Trondheim University Hospital, Norwegian University of Science and Technology
    Cephalalgia 20:804-20. 2000
    ..30, P = 0.05). VEP an AEP amplitudes, habituation, and amplitude-intensity function (ASF) slopes did not differ between groups when ANOVA main factors were considered...
  56. pmc Exonic splicing enhancer-dependent selection of the bovine papillomavirus type 1 nucleotide 3225 3' splice site can be rescued in a cell lacking splicing factor ASF/SF2 through activation of the phosphatidylinositol 3-kinase/Akt pathway
    Xuefeng Liu
    HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:2105-15. 2003
    ..Two exonic splicing enhancers, each containing two ASF/SF2 (alternative splicing factor/splicing factor 2) binding sites, are located between the two 3' splice sites and ..
  57. doi The role of Amicar in same-day anterior and posterior spinal fusion for idiopathic scoliosis
    George H Thompson
    Division of Pediatric Orthopaedics, Rainbow Babies and Children s Hospital, University Hospital Case Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA
    Spine (Phila Pa 1976) 33:2237-42. 2008
    ..A retrospective study of the effectiveness of Amicar (epsilon aminocaproic acid)...
  58. ncbi Multiubiquitin chain binding and protein degradation are mediated by distinct domains within the 26 S proteasome subunit Mcb1
    H Fu
    Cellular and Molecular Biology Program, University of Wisconsin Madison 53706, USA
    J Biol Chem 273:1970-81. 1998
    ..We have identified previously a candidate for one such receptor from diverse species, designated here as Mcb1 for Multiubiquitin chain-binding protein, based on its ability to bind Lys48-linked multiubiquitin chains and its ..
  59. ncbi hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B
    Alain Expert-Bezançon
    Centre de Genetique Moleculaire, CNRS UPR 2167, Laboratoire propre associe a l Universite Pierre et Marie Curie, 91198 Gif sur Yvette, France
    J Biol Chem 279:38249-59. 2004
    ..RNA affinity chromatography, we identified heterogeneous nuclear ribonucleoprotein (hnRNP) A1, the SR proteins ASF/SF2 and SC35, and hnRNP F/H as specific components that are assembled onto the G-rich element...
  60. ncbi A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples
    Montserrat Agüero
    Laboratorio Central Veterinario, Ministerio de Agricultura, Pesca y Alimentación, Algete, 28110 Madrid, Spain
    Vet Res 35:551-63. 2004
    ..start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF)...
  61. pmc Entamoeba invadens: the requirement for galactose ligands during encystment
    N A Turner
    Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010, USA
    Exp Parasitol 116:467-74. 2007
    ..The role of serum components (and the gal-terminated ligand asialofetuin; ASF) adsorbed onto the surface upon which encystment proceeds, and their effect on the multi-cellular aggregation ..
  62. ncbi Molecular monitoring of African swine fever virus using surveys targeted at adult ornithodoros ticks: a re-evaluation of Mkuze game reserve, South Africa
    L F Arnot
    Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Pretoria, 0002 South Africa
    Onderstepoort J Vet Res 76:385-92. 2009
    The Mkuze Game Reserve (MGR), in north-eastern KwaZulu-Natal Province, South Africa is an African swine fever virus (ASF) controlled area...
  63. ncbi Metastable Se6 as a ligand for Ag+: from isolated molecular to polymeric 1D and 2D structures
    Damian Aris
    Department of Chemistry, University of New Brunswick, Fredericton N B, E3B 6E2, Canada
    Dalton Trans 40:5865-80. 2011
    ..selenium led to crystals of 1/∞{[Ag(Se(6))](∞)[Ag(2)(SbF(6))(3)](∞)} 3 and {1/∞[Ag(Se(6))Ag](∞)}[AsF(6)](2)4. Pure bulk 4 was best prepared by the reaction of Se(4)[AsF(6)](2), silver metal and elemental selenium...
  64. ncbi Co-circulation of two genetically distinct viruses in an outbreak of African swine fever in Mozambique: no evidence for individual co-infection
    A D S Bastos
    Department of Zoology and Entomology, Mammal Research Institute, University of Pretoria, Pretoria 0002, South Africa
    Vet Microbiol 103:169-82. 2004
    ..first 3 weeks of quarantine of what appeared clinically and on post mortem examination to be African swine fever (ASF)...
  65. pmc Identification and characterization of a Drosophila proteasome regulatory network
    Josefin Lundgren
    Department of Molecular Biology and Functional Genomics, Stockholm University, S 106 91 Stockholm, Sweden
    Mol Cell Biol 25:4662-75. 2005
    ..in Drosophila melanogaster cell lines, RNA interference (RNAi)-mediated inhibition of the 26S proteasome subunit Rpn10/S5a and suppression of proteasome activity through treatment with active-site inhibitors...
  66. pmc Synthetic lethality of rpn11-1 rpn10Δ is linked to altered proteasome assembly and activity
    Abhishek Chandra
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Curr Genet 56:543-57. 2010
    ..Multiubiquitinated proteins interact with proteasome receptors, such as Rpn10, which intriguingly is also required for promoting proteasome stability...
  67. doi Intensity dependence of auditory evoked potentials during light interference in migraine
    Anna Ambrosini
    Headache Clinic, IRCCS INM Neuromed, Via Atinense, 18, I 86077 Pozzilli, Isernia, Italy
    Neurosci Lett 492:80-3. 2011
    ..IDAP was measured as the amplitude/stimulus intensity function (ASF) slope...
  68. pmc Structural, functional, and protein binding analyses of bovine papillomavirus type 1 exonic splicing enhancers
    Z M Zheng
    Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    J Virol 71:9096-107. 1997
    ..Substitution of U residues for either A or G residues in and around potential ASF/SF2 binding sites in SE1 or SE2 resulted in a significant reduction of splicing enhancer activity...
  69. ncbi Systematic analysis of longitudinal serological responses of pigs infected experimentally with African swine fever virus
    Ana Luísa Reis
    Instituto Gulbenkian de Ciencia, Apartado 14, 2781 901 Oeiras, Portugal
    J Gen Virol 88:2426-34. 2007
    ..In this study, the role of antibodies in the pathogenicity and diagnosis of African swine fever (ASF) was explored...
  70. doi Nanoemulsions of an anti-oxidant synergy formulation containing gamma tocopherol have enhanced bioavailability and anti-inflammatory properties
    Fonghsu Kuo
    Biomedical Engineering and Biotechnology Ph D Program, Center for Health and Disease Research, Division of NanoMedicine, University of Massachusetts Lowell, Lowell, MA 01854, USA
    Int J Pharm 363:206-13. 2008
    ..study investigated whether MicroFluidizer Processor-based nanoemulsions of an antioxidant synergy formulation (ASF), containing delta, alpha and gamma tocopherol influenced inflammation and bioavailability in CD-1 mice...
  71. pmc African swine fever virus isolate, Georgia, 2007
    Rebecca J Rowlands
    Institute for Animal Health, Pirbright, UK
    Emerg Infect Dis 14:1870-4. 2008
    African swine fever (ASF) is widespread in Africa but is rarely introduced to other continents. In June 2007, ASF was confirmed in the Caucasus region of Georgia, and it has since spread to neighboring countries...
  72. ncbi A unified approach for morphometric and functional data analysis in young, old, and demented adults using automated atlas-based head size normalization: reliability and validation against manual measurement of total intracranial volume
    Randy L Buckner
    Department of Psychology, HHMI at Washington University, Campus Box 1125, One Brookings Drive, St Louis, MO 63130, USA
    Neuroimage 23:724-38. 2004
    ..Automated atlas transformation generated the Atlas Scaling Factor (ASF) defined as the volume-scaling factor required to match each individual to the atlas target...
  73. doi Anterior spinal fusion versus posterior spinal fusion for moderate lumbar/thoracolumbar adolescent idiopathic scoliosis: a prospective study
    Yipeng Wang
    Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
    Spine (Phila Pa 1976) 33:2166-72. 2008
    ..A prospective study...
  74. ncbi Purification of poly-ubiquitinated proteins by S5a-affinity chromatography
    R Layfield
    School of Biomedical Sciences, University of Nottingham Medical School, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Proteomics 1:773-7. 2001
    ..The S5a subunit of the 26S proteasome binds poly-ubiquitin chains containing four or more ubiquitins...
  75. ncbi The hormone-sensitive lipase gene and body composition: the HERITAGE Family Study
    C Garenc
    Division of Kinesiology, Department of Preventive Medicine, Laval University, Ste Foy, Quebec, Canada
    Int J Obes Relat Metab Disord 26:220-7. 2002
    ....
  76. pmc Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1
    Nanette Rooke
    Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, 1602 Molecular Sciences Building, 405 Hilgard Avenue, Los Angeles, CA 90095, USA
    Mol Cell Biol 23:1874-84. 2003
    ..Proteins binding to this element were identified as hnRNP A1, hnRNP H, hnRNP F, and SF2/ASF by site-specific cross-linking and immunoprecipitation...
  77. ncbi Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: implications for pre-mRNA processing
    Laura K Zerbe
    Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, Colorado 802262, USA
    Mol Carcinog 41:187-96. 2004
    ..Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and alternative splicing factor/splicing factor 2 (ASF/SF2) act antagonistically to modulate splice site selection...
  78. doi Developmental-stage-specific regulation of the polyubiquitin receptors in Drosophila melanogaster
    Zoltán Lipinszki
    Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
    J Cell Sci 122:3083-92. 2009
    ..The stoichiometry of the proteasomal p54/Rpn10 polyubiquitin receptor subunit, relative to that of other regulatory particle (RP) subunits falls suddenly at the ..
  79. ncbi One-step and two-step spin-crossover iron(II) complexes of ((2-methylimidazol-4-yl)methylidene)histamine
    Tetsuya Sato
    Department of Chemistry, Faculty of Science, Kumamoto University, Kurokami 2 39 1, Kumamoto 860 8555, Japan
    Inorg Chem 48:7211-29. 2009
    ..solvent = 2-PrOH and CH(3)CN) and [Fe(H(2)L(2-Me))(2)]X x Y x solvent (X = Cl and Br; Y = ClO(4), BF(4), PF(6), and AsF(6); solvent = EtOH and 2-PrOH)...
  80. doi Total extract and standardized fraction from the stem bark of Butea monosperma have osteoprotective action: evidence for the nonestrogenic osteogenic effect of the standardized fraction
    Rashmi Pandey
    Division of Endocrinology, Central Drug Research Institute Council of Scientific and Industrial Research, Lucknow, India
    Menopause 17:602-10. 2010
    ..of this study was to determine the skeletal effects of Butea total extract (BTE) and its acetone soluble fraction (ASF) from Butea monosperma, which is rich in methoxyisoflavones, in ovariectomized (OVx) rats, a model for ..
  81. ncbi The RNA splicing factor ASF/SF2 inhibits human topoisomerase I mediated DNA relaxation
    Felicie F Andersen
    Department of Molecular and Structural Biology, University of Aarhus, C F Møllers Alle, Denmark
    J Mol Biol 322:677-86. 2002
    Human topoisomerase I interacts with and phosphorylates the SR-family of RNA splicing factors, including ASF/SF2, and has been suggested to play an important role in the regulation of RNA splicing...
  82. ncbi Binding surface mapping of intra- and interdomain interactions among hHR23B, ubiquitin, and polyubiquitin binding site 2 of S5a
    Kyoung Seok Ryu
    Yusong gu, Gusong Dong 373 1, Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejon 305 701, South Korea
    J Biol Chem 278:36621-7. 2003
    ..On the other hand, the UbL domain interacts with the poly-Ub binding site 2 (PUbS2) domain of the S5a protein, which can carry polyubiquitinated substrates into the proteasome...
  83. ncbi Pig major acute-phase protein and apolipoprotein A-I responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky's disease
    Rakel Carpintero
    Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain
    Vet Res 38:741-53. 2007
    ..The animals were experimentally infected with African Swine Fever (ASF) or Aujeszky's disease (AD) viruses...
  84. ncbi A novel Physarum polycephalum SR protein kinase specifically phosphorylates the RS domain of the human SR protein, ASF/SF2
    Shide Liu
    Shenzhen Key Laboratory of Microbial and Genetic Engineering, College of Life Science Shenzhen University, Shenzhen, China
    Acta Biochim Biophys Sin (Shanghai) 41:657-67. 2009
    ..vitro phosphorylation assay to investigate the phosphorylation effect of recombinant PSRPK on the human SR protein, ASF/SF2. Western blot analysis showed that PSRPK could phosphorylate ASF/SF2 in E. coli cells...
  85. doi Molecular characterisation of African swine fever viruses from Nigeria (2003-2006) recovers multiple virus variants and reaffirms CVR epidemiological utility
    Olajide A Owolodun
    Department of Biochemistry and AMB, National Veterinary Research Institute, Vom, Plateau State, Nigeria
    Virus Genes 41:361-8. 2010
    ..from wild and domestic suids in Nigeria, over a 3-year period (2003-2006), were evaluated for African swine fever (ASF) virus genome presence by targeting three discrete genome regions, namely the 478-bp C-terminal p72 gene region ..
  86. ncbi Xe3OF3(+), a precursor to a noble-gas nitrate; syntheses and structural characterizations of FXeONO2, XeF2·HNO3, and XeF2·N2O4
    Matthew D Moran
    Department of Chemistry, McMaster University, Hamilton, Ontario L8S 4M1, Canada
    J Am Chem Soc 132:13823-39. 2010
    Xenon fluoride nitrate has been synthesized by reaction of NO(2)F with [FXeOXeFXeF][AsF(6)] at -50 °C...
  87. pmc Regulation of human neurotropic JC virus replication by alternative splicing factor SF2/ASF in glial cells
    Ilker Kudret Sariyer
    Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 6:e14630. 2011
    ....
  88. ncbi Expression of alphasmooth muscle actin in lens epithelia from human donors and cataract patients
    Elisabeth Rungger-Brandle
    Cell Biology Laboratory, University Eye Clinic, 22, Rue Alcide Jentzer, CH 1211 Geneve, Switzerland
    Exp Eye Res 81:539-50. 2005
    ..cataract (CAT), and cataractous lenses with posterior subcapsular opacities (PSO) or anterior subcapsular fibrosis (ASF)...
  89. ncbi Wax-matrix tablet for time-dependent colon-specific delivery system of sophora flavescens Aiton: preparation and in vivo evaluation
    Meijuan Zou
    School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P R China
    Drug Dev Ind Pharm 35:224-33. 2009
    ..expanding agent, carboxymethyl starch sodium (CMS-Na), and NaCl was prepared, and Sophora flavescens Aiton (ASF, extracts of traditional Chinese medicine) was used as model drug...
  90. pmc HIV Rev-dependent binding of SF2/ASF to the Rev response element: possible role in Rev-mediated inhibition of HIV RNA splicing
    D M Powell
    Gladstone Institute of Virology and Immunology, University of California, San Francisco 94141 9100, USA
    Proc Natl Acad Sci U S A 94:973-8. 1997
    ..Our studies now demonstrate that at least one member of the SR family of splicing factors, SF2/ASF, specifically binds to a subregion of the RRE in vitro in a Rev-dependent manner...
  91. ncbi The A-site finger in 23 S rRNA acts as a functional attenuator for translocation
    Taeko Komoda
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 281:32303-9. 2006
    Helix 38 (H38) in 23 S rRNA, which is known as the "A-site finger (ASF)," is located in the intersubunit space of the ribosomal 50 S subunit and, together with protein S13 in the 30 S subunit, it forms bridge B1a...
  92. doi Bone morphogenetic protein (RhBMP-2) as a substitute for iliac crest bone graft in multilevel adult spinal deformity surgery: minimum two-year evaluation of fusion
    Daniel S Mulconrey
    Department of Orthopaedic Surgery, Barnes Jewish Hospital and Washington University School of Medicine, St Louis, Missouri, USA
    Spine (Phila Pa 1976) 33:2153-9. 2008
    ..Prospective, single center, nonblinded radiographic analysis of anterior and posterior adult spinal deformity fusions performed with bone morphogenetic protein (rhBMP-2)...
  93. ncbi Modulation of the expression of p16INK4a and p14ARF by hnRNP A1 and A2 RNA binding proteins: implications for cellular senescence
    Deguang Zhu
    Department of Biology, The City College of CUNY, New York, New York 10031, USA
    J Cell Physiol 193:19-25. 2002
    ..We have found that increasing the ratio of either hnRNP A1 or A2 over that of splicing factor SF2/ASF results in the preferential generation of the p14(ARF) isoform...
  94. ncbi Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins
    Ming Chih Lai
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
    J Biol Chem 279:31745-9. 2004
    ..export receptor TAP associates preferentially with the hypophosphorylated form of shuttling SR proteins, including ASF/SF2...
  95. ncbi CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing
    Hiroshi Maita
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Exp Cell Res 303:375-87. 2005
    ..and studied their subcellular localizations and interactions with PAP-1 and other SR proteins, including SC35, SF2/ASF, and U2AF35...
  96. ncbi Cerebral information processing in personality disorders: I. Intensity dependence of auditory evoked potentials
    Wei Wang
    Division of Neuropsychology and Psychotherapy, Anhui Institute of Stereotactic Neurosurgery, Anhui Provincial Hospital, Hefei, China
    Psychiatry Res 141:173-83. 2006
    ..In addition, the histrionic group showed steeper amplitude/stimulus intensity function (ASF) slopes at three midline scalp electrodes than the healthy controls or the other patient groups...
  97. pmc Molecular anatomy of a speckle
    Lisa L Hall
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA
    Anat Rec A Discov Mol Cell Evol Biol 288:664-75. 2006
    ..Conditions that disperse SC35 also disperse poly(A) RNA, whereas the splicing factor ASF/SF2 can be dispersed under conditions in which SC35 or SRm300 remain as intact components of a core domain.
  98. ncbi Relationship between apical membrane elasticity and stress fiber organization in fibroblasts analyzed by fluorescence and atomic force microscopy
    S Kidoaki
    Division of Biomedical Engineering, Graduate School of Medicine, Kyushu University, Fukuoka 812 8582, Japan
    Biomech Model Mechanobiol 5:263-72. 2006
    ..of the upper nuclear region decreased with increased SF density in whole cells and the degree of orientation of ASF, although the pseudopodium region did not exhibit such a trend...
  99. ncbi Quantitative analysis of in vitro ubiquitinated cyclin B1 reveals complex chain topology
    Donald S Kirkpatrick
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Nat Cell Biol 8:700-10. 2006
    ..Together, our observations expand the context of what can be considered to be a sufficient degradation signal and provide unique insights into the mechanisms of substrate ubiquitination...
  100. ncbi SR protein kinase 1 is resilient to inactivation
    Jacky Chi Ki Ngo
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    Structure 15:123-33. 2007
    ..is a constitutively active kinase, which processively phosphorylates multiple serines within its substrates, ASF/SF2...
  101. pmc The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x
    Maria Paola Paronetto
    Department of Public Health and Cell Biology, Section of Anatomy, University of Rome Tor Vergata, 00133 Rome, Italy
    J Cell Biol 176:929-39. 2007
    ..Moreover, coexpression of ASF/SF2 with Sam68, or fusion with an RS domain, counteracted Sam68 splicing activity toward Bcl-x...

Research Grants63

  1. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
    ..For example, the gene encoding the splicing factor SF2/ASF is a proto-oncogene that can be activated by gene amplification in breast cancer...
  2. ALBERT HONG JAE KIM; Fiscal Year: 2016
    ..The first aim will define an exciting link between Cdc20-APC and the S5a subunit of the 26S proteasome, a multisubunit complex designed to destroy ubiquitinated substrates, in dendrite ..
  3. Molecular Mechanisms that Cause Muscle Atrophy
    Alfred L Goldberg; Fiscal Year: 2012
    ..We have identified new protein partners of MuRF1 (S5a and novel E2s) that enhance MuRF1- dependent proteolysis in vitro and protein substrates (including critical ..
  4. Steven R Grossman; Fiscal Year: 2014
    ..hHR23 proteins, in turn, are recognized by the S5a subunit of the proteasome, which may be the gateway to the proteasome for p53, leading to its destruction...
  5. SYSTEMATIC ANALYSIS OF PROTEOLYSIS PATHWAYS FOR CULLIN TARGETS
    JEFFREY WADE HARPER; Fiscal Year: 2012
    ..Using the Global Protein Stability (GPS) system, we will identify targets of the proteasome-associated Rpn10 "facilitator" protein from a library of cells expressing 12,000 human genes, thereby defining the repertoire of ..
  6. NMR Structural Studies of Ubiquitin Receptor Protein Complexes
    Kylie J Walters; Fiscal Year: 2013
    ..to their degradation, as their initial interaction with the proteasome is through RP ubiquitin receptors, S5a and Rpn13...
  7. Ilker Kudret Sariyer; Fiscal Year: 2016
    ..We recently identified the alternative splicing factor, SF2/ASF, as a potential regulator of JCV as its overexpression in glial cells strongly suppresses viral gene expression and ..
  8. VAISHALI MOULTON; Fiscal Year: 2016
    ..a promising candidate is the serine arginine (SR) family member Alternative Splicing Factor/Splicing Factor 2 (ASF/SF2)...
  9. ASF/SF2: Phosphorylation and RNA Binding
    Gourisankar Ghosh; Fiscal Year: 2012
    Alternating splicing factor/splicing factor 2 (ASF/SF2), a member of the serine-arginine (SR) protein family, plays important roles both in constitutive and alternative pre-mRNA splicing...
  10. Regulation of tumorigenic spliced isoforms in cancer
    Aixa Tapia Santos; Fiscal Year: 2010
    ..Mutations affecting sequences specific for the binding of splicing factors SC35 and SF2/ASF in exon 11 result in changes in the MDM2 alternative spliced isoform, both in normal and in stress conditions...
  11. Gross Hall Animal Resource Center
    Susan V Bryant; Fiscal Year: 2010
    ..requests funds to build-out and equip 23,385 gross square feet (GSF) of shell space at the UCI to create the 17,433 ASF Gross Hall Animal Resource Center (GHARC) to support PHS-funded researchers...
  12. James L Manley; Fiscal Year: 2015
    ..An ASF/SF2-CTD fusion protein has been used to show that the CTD can enhance splicing in vitro in the presence of a ..
  13. Splicing factors as therapeutic targets for the treatment of ovarian cancer
    William T Beck; Fiscal Year: 2012
    ..Indeed, several splicing factors, including PTB and SRp20, are overexpressed in tumors and one, SF2/ASF, was shown to be an oncogene...
  14. BLANTON SMITH TOLBERT; Fiscal Year: 2016
    ..to identify the molecular principles that HIV uses to co-opt the mutually antagonistic factors, hnRNP A1 (A1) and ASF, in order to regulate its 3'splice site A7 (ssA7)...
  15. STEWART H SHUMAN; Fiscal Year: 2016
    ..in sexual differentiation and vegetative growth;and (iii) the ability to bypass "CTD pathologies" caused by S2A and S5A mutations...
  16. Mechanism of APOBEC3-Mediated Innate Immunity to HIV-1
    Yong Hui Zheng; Fiscal Year: 2012
    ..This subfamily includes ASA,A3B, A3C, A3DE, ASF, A3G and ASH, which belong to the cytidine deaminase family...
  17. Development of a Novel Gastrointestinal Radiomitigator
    Gabor Tigyi; Fiscal Year: 2009
    ..in adult Rhesus macaques is consistent with the low toxicity we determined in mice (ICH guidance S2A, S2B, S4A, S5A &S5B)...
  18. ALAN KENNETH PERCY; Fiscal Year: 2016
    ..have active affiliation with the International Rett Syndrome Foundation (IRSF), the Angelman Syndrome Foundation (ASF), and the Prader-Willi Syndrome Association (PWSA). A website for this RDCRC is available at www.circ.uab.edu...
  19. Sphingolipid Regulation of Caspase 9 Alternative Splicing
    Charles Chalfant; Fiscal Year: 2009
    ..laboratory disclose that the alternative splicing of caspase 9 is intrinsically linked to the SR protein, SRp30a (ASF/SF2)...
  20. The Alport Syndrome Symposium for Physicians, Researchers and Families
    Clifford E Kashtan; Fiscal Year: 2010
    ..and Outcomes Registry (ASTOR), located at the University of Minnesota, and the Alport Syndrome Foundation (ASF)...
  21. HUMAN CYTOMEGALOVIRUS TEGUMENT PROTEINS
    Thomas E Shenk; Fiscal Year: 2013
    ..it is required for efficient replication in epithelial cells, and we have shown that it binds to the cellular SF2/ASF protein, a multi-functional splicing factor...
  22. Gregory J Phillips; Fiscal Year: 2016
    ..simplified mouse model whose GI tract is colonized with only eight bacterial species, the altered Schaedler flora (ASF). Collectively, the ASF facilitates normal physiological function and health of the mouse GI system...
  23. Rodent Holding for WIMR Cardiovascular Research
    Richard L Moss; Fiscal Year: 2013
    ..We seek to outfit three currently unequipped animal housing rooms (total 696 assignable square feet, ASF) with high-density caging and to properly equip the two adjacent procedure rooms (total 222 ASF)...
  24. Mechanism of SR Protein Binding to the Splicing Kinase SRPK1
    RYAN MATTHEW PLOCINIK; Fiscal Year: 2011
    ..Recent kinetic and crystallographic studies indicate that SRPK1 phosphorylates the RS domain of the SR protein, ASF/SF2, using a mechanism that is directional, regiospecific, and processive...
  25. PAR04-122, Extramural Research Facilities Improvement P*
    Michael R Grever; Fiscal Year: 2010
    ..The specific renovation project being proposed is for the "buildout" of a floor of the BRT (approximately 24,000 asf) to centralize and support one of the fastest growing and developing areas of cancer research, the Experimental ..
  26. Angiocidin, a Novel Differentiation Agent for the Treatment of Leukemia
    Charles S Swindell; Fiscal Year: 2013
    ..by applicant): This Phase 1 SBIR project is to perform go-/no-go evaluation of the proprietary protein, angiocidin, as a novel therapy for the treatment of acute myeloid leukemia (AML)...
  27. Mouse Caging System for the University of California Davis
    KC KENT LLOYD; Fiscal Year: 2010
    ..In anticipation of an award, UC Davis has provided a dedicated, 20,000 ASF laboratory building, including a 6000 sq ft, 5-room mouse vivarium, for mouse-related research and resource ..
  28. REGULATION OF ALTERNATIVE PROCESSING OF CALCITONIN/CGRP
    Susan Berget; Fiscal Year: 2002
    ..that the enhancer has a CORE resembling a pseudo exon and binds splicing factors, including U1 snRNPs, SR proteins (ASF/SF2 and SRp20), and PTB...
  29. Cardiovascular and Regenerative Sciences in WIMR Phase II
    ROBERT NEAL GOLDEN; Fiscal Year: 2010
    ..we will fully build-out and equip one floor of WIMR II, providing more than 25,000 assignable square feet (ASF) of highly integrated space...
  30. Nanospheres as Vehicles for Treatment of Neuroblastoma
    Thomas Shea; Fiscal Year: 2004
    ..In our ongoing studies we have developed an anti-oxidant synergistic formulation ("ASF"), comprised of alpha-tocopherol (vitamin E), sodium pyruvate and phosphatidyl choline, that supports axonal ..
  31. NEUROENDOCRINE REGULATION OF ENDOCRINE GLANDS
    NOELLE GRANGER; Fiscal Year: 1992
    ..factor (JH III ATF), which specifically stimulates the synthesis of JH III, and a JH I allatostatic factor (JH I ASF) which inhibits the synthesis of JH I...
  32. ANGIOCIDIN, A NEW ANGIOGENESIS INHIBITOR
    George Tuszynski; Fiscal Year: 2004
    ..The protein, termed angiocidin, inhibited endothelial cell viability and tube formation in vitro and prevented the growth of tumors in vivo...
  33. RR-03-011, Extramural Research Facilities Construction *
    Dallas Hyde; Fiscal Year: 2004
    ..The building will include 5,992 assignable square feet (ASF) of laboratory research space, 178 ASF of research support space and 1,200 ASF of office space...
  34. EXTRAMURAL RESEARCH FACILITIES CONSTRUCTION
    RIGHARD KRUGMAN; Fiscal Year: 2004
    This application requests $3,221,187 of matching funds to finish 31,000 asf/45,400 gsf on the second and third floors of the Davis building...
  35. Rederivation and Internal Quarantine Suite (RIQS)
    JEFFREY GOODWIN; Fiscal Year: 2009
    ..provided by applicant): This animal resource improvement application requests funds to renovate and equip 2,332 ASF of existing vivarium and procedure space at the University of California, Irvine (UCI) to create the Rederivation &..
  36. Novel Assays to Identify HIV Vif Inhibitors
    Dana Gabuzda; Fiscal Year: 2006
    ..Vif protein is required during virus replication to overcome the antiviral activity of APOBEC3G (A3G) and APOBEC3F (ASF), cytidine deaminases that induce G to A hypermutation in newly synthesized viral DNA...
  37. STAT FACTOR AND SIGNAL TRANSDUCTION AND CANCER
    Xin Yuan Fu; Fiscal Year: 2000
    ..Manley's laboratory at Columbia University, where he was the first to identify the alternative splicing factor, ASF. When he was a postdoctoral fellow with Dr...
  38. Design of molecules that promote SMN2 exon 7 inclusion
    ADRIAN KRAINER; Fiscal Year: 2005
    ..An SF2/ASF-dependent exonic splicing enhancer in exon 7 appears to be defective in SMN2, and recognition of this exon is very ..
  39. THE ROLE OF NUB1 IN PATHOGENESIS OF PARKINSON'S DISEASE
    Tetsu Kamitani; Fiscal Year: 2009
    ..a novel NEDD8-interacting protein, NUB1, that functioned like a molecular chaperone to recruit NEDD8 to the S5a subunit of the 26S proteasome for degradation...
  40. AUTOSTEREO FIBERSCOPE FOR 3-D MONITORING OF TUMORS
    WINSTON HO; Fiscal Year: 1993
    ..even more accurate diagnoses, Physical Optics Corporation (POC) proposes to develop an autostereo fiberscope (ASF) for real-time 3-D monitoring of cancerous tumors, in vivo, without the need for open surgery...
  41. Formation of Heterochromatin and Cellular Senescence Driven by HIRA and ASF 1a
    Gregory Enders; Fiscal Year: 2009
    ..Specific Aim 3. Investigate the role of GSK3 activity in localization of HIRA to PML bodies, formation of SAHF and onset of senescence. ..
  42. Microecology-murine gut-initiation & progression of IBD
    James Fox; Fiscal Year: 2005
    ..A standardized microbiota used in colonizing germfree rodents referred to "Altered Schaedler Flora" (ASF) was developed...
  43. FMRI FACILITY FOR THE BRAIN IMAGING CENTER
    David Bailey; Fiscal Year: 1999
    ..through gifts, campus discretionary and loan funds to support construction of a 4,390 assignable square foot (ASF) facility as an addition to the School's Basic Science Building...
  44. Identification and analysis of substrate activators of the Aurora B kinase
    JILL SCHUMACHER; Fiscal Year: 2009
    ..elegans cell cycle we will: 1) Determine the functional relationship between TLK-1, ASF-1, ICP-1, and AIR-2 in chromosome behavior, 2) Define the molecular differences between the TLK-1/ICP-1/AIR-2 ..
  45. FACILITIES CONSTRUCTION CENTERALIZED PHARMCOGENOMICS
    Palmer Taylor; Fiscal Year: 2003
    In this rewritten and resubmitted application, funds are requested to match State of California funds for an 8,059 ASF Centralized Pharmacogenomics and Toxicogenomics Animal Facility in a new pharmaceutical sciences building...
  46. EXTRAMURAL FACILITIES IMPROVEMENT PROGRAM
    Robert Golden; Fiscal Year: 2004
    ..matching support from its discretionary resources, the University of Wisconsin Medical School will construct 24,336 ASF of space devoted to translational cancer research in a new Interdisciplinary Research Complex (IRC)...
  47. Ventilated Mouse Cages for NIH Funded Construction Projects
    DENNIS CUNNINGHAM; Fiscal Year: 2006
    ..Neuroscience Research Facility and central to recent state-of-the-art laboratory construction, including two 50,000 ASF laboratory buildings (Sprague and Hewitt Hall), the requested 2,268 mouse cages, installed in a combined holding ..
  48. SPLICING ENHANCER REGULATION OF GROWTH HORMONE ISOFORMS
    Fritz Rottman; Fiscal Year: 1999
    ..the precise nucleotide sequence of the bGH exonic enhancer, and the role of important functional domains of SF2/ASF (RNA binding and Arg/Ser domains) will be defined. 2...
  49. EXTRAMURAL RESEARCH FACILITIES CONSTRUCTION
    MARTIN CADWALLADER; Fiscal Year: 2004
    ..8) Purchase of equipment: primate caging and cage washer Completion of this project (6,061 ASF) will enable efficient expansion and greater proximity of PHS supported research-using NHP, particularly in areas ..
  50. Structural Analysis of NAT Acetylation, Substrate Specificity and Polymorphisms
    Kylie J Walters; Fiscal Year: 2010
    ..We determine how certain arylamines evade NAT detoxification and why the NAT proteins of some people are destroyed before they can perform their protein function. ..
  51. CREB in Differentiation of Bronchial Mucous Cells
    Ja Seok Koo; Fiscal Year: 2007
    ..This application will address this hypothesis by determining the molecular mechanisms whereby CREB regulates normal and aberrant bronchial epithelial cell differentiation. ..
  52. Diet, Activity, Obesity & The Built Environment Dynamics
    Barry Popkin; Fiscal Year: 2009
    ..A central issue is determining modifiable environment factors that may reduce ethnic and socioeconomic differentials in health status. ..
  53. Physical Environment Dynamics, Inequality and Obesity
    Barry Popkin; Fiscal Year: 2006
    ..We will also consider issues related to sample selectivity, within race heterogeneity and acculturation. ..
  54. CHILD HEALTH EFFECTS OF RAPID SOCIO-DEMOGRAPHIC CHANGE
    Barry Popkin; Fiscal Year: 2004
    ..Finally, we will compare results across countries to determine whether there is a consistent pattern or structure of relationships across countries at different levels of development. ..
  55. An Interdisciplinary Strategy for Obesity (RMI)
    Barry Popkin; Fiscal Year: 2006
    ..We will apply the knowledge gained from our interdisciplinary research to achieve our long-term goal of developing novel treatment and prevention interventions for obesity. [unreadable] [unreadable]..
  56. BEYOND RACE--EXPLAINING INEQUALITY MANIFESTED AS OBESITY
    Barry Popkin; Fiscal Year: 2003
    ..This work will provide important insights in the relative importance of genetic and environmental determinants of overweight and activity. ..
  57. REGULATION OF THE STIMULATION OF MUCIN GENE EXPRESSION A
    Ja Seok Koo; Fiscal Year: 2003
    ....
  58. Biochemical Characterization of Parkin
    Kiran Madura; Fiscal Year: 2003
    ..iv). Our long-term objective is to characterize the ubiquitination of a-Syn and other physiological substrates by Parkin. ..
  59. ABI 3100 Genetic Analyzer for Nucleic Acid Sequencing
    Arthur Haas; Fiscal Year: 2003
    ..In addition, the proposed instrument will allow longer reads than currently available on the ABI 310 instrument. ..
  60. CARDIOVASCULAR NUTRITION IN MEDICAL EDUCATION
    Scott Grundy; Fiscal Year: 2002
    ..The long term goal is to produce graduates who routinely address nutrition and exercise for cardiovascular health with their patients. ..
  61. "Studies to examine Centrin's role in DNA repair"
    Kiran Madura; Fiscal Year: 2010
    ..However, the function of Rad4 is not well understood, and its characterization is the focus of this application. ..
  62. Targeting a novel silencer to correct SMN2 splicing in Spinal Muscular Atrophy
    Ravindra Singh; Fiscal Year: 2007
    ..To explore the therapeutic potential of antisense oligos, we will extend our study to the mice models of SMA. [unreadable] [unreadable] [unreadable]..
  63. ATP-Ubiquitin Dependent Proteolysis
    Arthur L Haas; Fiscal Year: 2011
    ....