PRKAG3

Summary

Gene Symbol: PRKAG3
Description: protein kinase AMP-activated non-catalytic subunit gamma 3
Alias: AMPKG3, 5'-AMP-activated protein kinase subunit gamma-3, 5'-AMP-activated protein kinase, gamma-3 subunit, AMPK gamma-3 chain, AMPK gamma3, AMPK subunit gamma-3, protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Species: human

Top Publications

  1. pmc Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding
    P C Cheung
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U K
    Biochem J 346:659-69. 2000
  2. ncbi AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism
    Barbara B Kahn
    Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Cell Metab 1:15-25. 2005
  3. ncbi Expression profiling of the gamma-subunit isoforms of AMP-activated protein kinase suggests a major role for gamma3 in white skeletal muscle
    Margit Mahlapuu
    Arexis AB, Gothenburg, Sweden
    Am J Physiol Endocrinol Metab 286:E194-200. 2004
  4. ncbi 5'-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle
    Jakob N Nielsen
    Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Denmark
    J Appl Physiol (1985) 94:631-41. 2003
  5. ncbi A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle
    D Milan
    Laboratoire de Genetique Cellulaire, Institut National de la Recherche Agronomique INRA, 31326 Castanet Tolosan, France
    Science 288:1248-51. 2000
  6. ncbi Changes in exercise-induced gene expression in 5'-AMP-activated protein kinase gamma3-null and gamma3 R225Q transgenic mice
    Brian R Barnes
    Department of Physiology and Pharmacology, Karolinska Institutet, von Eulers vag 4, 4th Floor, S 171 77 Stockholm, Sweden
    Diabetes 54:3484-9. 2005
  7. pmc Polymorphisms in epigenetic and meat quality related genes in fourteen cattle breeds and association with beef quality and carcass traits
    Xuan Liu
    Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
    Asian-Australas J Anim Sci 28:467-75. 2015
  8. ncbi Effects of Chromium-Loaded Chitosan Nanoparticles on Glucose Transporter 4, Relevant mRNA, and Proteins of Phosphatidylinositol 3-Kinase, Akt2-Kinase, and AMP-Activated Protein Kinase of Skeletal Muscles in Finishing Pigs
    Lujie Liu
    Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People s Republic of China
    Biol Trace Elem Res . 2016
  9. pmc A kinesin-1 binding motif in vaccinia virus that is widespread throughout the human genome
    Mark P Dodding
    Cell Motility Laboratory, London Research Institute, Cancer Research UK, London, UK
    EMBO J 30:4523-38. 2011
  10. doi PRKAG3 and CAST genetic polymorphisms and quality traits of dry-cured hams--II. Associations in French dry-cured ham Jambon de Bayonne and their dependence on salt reduction
    V Sante-Lhoutellier
    INRA, UR 370 QuaPA, 63122 Saint Genes Champanelle, France
    Meat Sci 92:354-9. 2012

Research Grants

Scientific Experts

Detail Information

Publications83

  1. pmc Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding
    P C Cheung
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U K
    Biochem J 346:659-69. 2000
    ..Labelling studies, using the reactive AMP analogue 8-azido-[(32)P]AMP, indicate that the gamma subunit may participate directly in the binding of AMP within the complex...
  2. ncbi AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism
    Barbara B Kahn
    Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Cell Metab 1:15-25. 2005
    ..Through signaling, metabolic, and gene expression effects, AMPK enhances insulin sensitivity and fosters a metabolic milieu that may reduce the risk for obesity and type 2 diabetes...
  3. ncbi Expression profiling of the gamma-subunit isoforms of AMP-activated protein kinase suggests a major role for gamma3 in white skeletal muscle
    Margit Mahlapuu
    Arexis AB, Gothenburg, Sweden
    Am J Physiol Endocrinol Metab 286:E194-200. 2004
    ..The observation that the AMPKgamma3 isoform is expressed primarily in white skeletal muscle, in which it is the predominant gamma-isoform, strongly suggests that gamma3 has a key role in this tissue...
  4. ncbi 5'-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle
    Jakob N Nielsen
    Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Denmark
    J Appl Physiol (1985) 94:631-41. 2003
    ..01). In conclusion, trained human skeletal muscle has increased alpha(1)-AMPK protein levels and blunted AMPK activation during exercise...
  5. ncbi A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle
    D Milan
    Laboratoire de Genetique Cellulaire, Institut National de la Recherche Agronomique INRA, 31326 Castanet Tolosan, France
    Science 288:1248-51. 2000
    ..Here, it is shown that the mutation is a nonconservative substitution (R200Q) in the PRKAG3 gene, which encodes a muscle-specific isoform of the regulatory gamma subunit of adenosine monophosphate-activated ..
  6. ncbi Changes in exercise-induced gene expression in 5'-AMP-activated protein kinase gamma3-null and gamma3 R225Q transgenic mice
    Brian R Barnes
    Department of Physiology and Pharmacology, Karolinska Institutet, von Eulers vag 4, 4th Floor, S 171 77 Stockholm, Sweden
    Diabetes 54:3484-9. 2005
    ..We used AMPKgamma3 mutant-overexpressing Tg-Prkag3(225Q) and AMPKgamma3-knockout Prkag3-/- mice to determine the role of the AMPKgamma3 isoform in exercise-induced ..
  7. pmc Polymorphisms in epigenetic and meat quality related genes in fourteen cattle breeds and association with beef quality and carcass traits
    Xuan Liu
    Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
    Asian-Australas J Anim Sci 28:467-75. 2015
    ..genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in ..
  8. ncbi Effects of Chromium-Loaded Chitosan Nanoparticles on Glucose Transporter 4, Relevant mRNA, and Proteins of Phosphatidylinositol 3-Kinase, Akt2-Kinase, and AMP-Activated Protein Kinase of Skeletal Muscles in Finishing Pigs
    Lujie Liu
    Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People s Republic of China
    Biol Trace Elem Res . 2016
    ..05) in 200 μg/kg treatment group. The mRNA of AMPK subunit gamma-3 (PRKAG3) and protein of AMPKα1 was significantly increased (P < 0.001) with the addition of Cr-CNP...
  9. pmc A kinesin-1 binding motif in vaccinia virus that is widespread throughout the human genome
    Mark P Dodding
    Cell Motility Laboratory, London Research Institute, Cancer Research UK, London, UK
    EMBO J 30:4523-38. 2011
    ..Our observations demonstrate that KLC binding can be conferred by a common set of features that are found in a wide range of proteins associated with diverse cellular functions and human diseases...
  10. doi PRKAG3 and CAST genetic polymorphisms and quality traits of dry-cured hams--II. Associations in French dry-cured ham Jambon de Bayonne and their dependence on salt reduction
    V Sante-Lhoutellier
    INRA, UR 370 QuaPA, 63122 Saint Genes Champanelle, France
    Meat Sci 92:354-9. 2012
    This study aimed to evaluate the effects of PRKAG3 Ile199Val and CAST Arg249Lys and CAST Ser638Arg polymorphisms on the quality traits of the French dry-cured ham Jambon de Bayonne and their interaction with salt reduction...
  11. pmc Association of AMP-activated protein kinase with risk and progression of non-Hodgkin lymphoma
    Aaron E Hoffman
    Department of Epidemiology, Tulane School of Public Health and Tropical Medicine and Tulane Cancer Center, New Orleans, LA, USA
    Cancer Epidemiol Biomarkers Prev 22:736-44. 2013
    ....
  12. doi Evidence for three highly significant QTL for meat quality traits in the Finnish Yorkshire pig breed
    P Uimari
    Agrifood Research Finland, MTT, Biotechnology and Food Research, FI 36100 Jokioinen, Finland
    J Anim Sci 91:2001-11. 2013
    ..Three genomic regions were significant for the meat quality traits. The PRKAG3 region on chromosome 15 was significant for pH measured from loin and ham and for a* (redness) measured from loin...
  13. doi Joint analysis of additive, dominant and first-order epistatic effects of four genes (IGF2, MC4R, PRKAG3 and LEPR) with known effects on fat content and fat distribution in pigs
    P Lopez-Buesa
    Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, Zaragoza, 50013, Spain
    Anim Genet 45:133-7. 2014
    LEPR, MC4R, IGF2 and PRKAG3 are genes with known effects on fat content and distribution in pig carcass and pork...
  14. pmc A combination of two variants in PRKAG3 is needed for a positive effect on meat quality in pigs
    Pekka Uimari
    MTT Agrifood Research Finland, Biotechnology and Food Research, FI 31600 Jokioinen, Finland
    BMC Genet 15:29. 2014
    ..Several amino acid substitutions in PRKAG3 have been associated with various meat quality traits...
  15. doi Association of AMPK subunit gene polymorphisms with growth, feed intake, and feed efficiency in meat-type chickens
    Sihua Jin
    National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
    Poult Sci 95:1492-7. 2016
    ..reported SNPs in the chicken adenosine monophosphate activated protein kinase (AMPK) subunits PRKAB1, PRKAG2, and PRKAG3 genes with body weight (BW), body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) in two ..
  16. pmc Genome-wide association studies (GWAS) identify a QTL close to PRKAG3 affecting meat pH and colour in crossbred commercial pigs
    Chunyan Zhang
    Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, AB, T6G 2P5, Canada
    BMC Genet 16:33. 2015
    ..GWAS have become a state-of-the-art approach to genetically improve economically important traits. However, GWAS focused on pork quality are still relatively rare...
  17. doi Variation in the ovine PRKAG3 gene
    Guo Yang
    Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China Gene Marker Laboratory, Faculty of Agriculture and Life Sciences, P O Box 84, Lincoln University, Lincoln 7647, New Zealand
    Gene 567:251-4. 2015
    ..The PRKAG3 gene (PRKAG3) encodes the γ3 subunit of the AMPK...
  18. doi Hypoxia-Induced Upregulation of miR-132 Promotes Schwann Cell Migration After Sciatic Nerve Injury by Targeting PRKAG3
    Chun Yao
    Jiangsu Key Laboratory of Neuroregeneration, Co innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China
    Mol Neurobiol 53:5129-39. 2016
    ..miR-132 downregulated PRKAG3 through binding to its 3'-UTR, and PRKAG3 knockdown compromised the reducing effect of miR-132 inhibition on SC ..
  19. pmc AMPKγ3 is dispensable for skeletal muscle hypertrophy induced by functional overload
    Isabelle Riedl
    Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Am J Physiol Endocrinol Metab 310:E461-72. 2016
    ..Using AMPKγ3 mutant-overexpressing transgenic Tg-Prkag3(225Q) and AMPKγ3-knockout (Prkag3(-/-)) mice, we investigated the requirement for the AMPKγ3 isoform in ..
  20. doi Effect of candidate gene polymorphisms on reproductive traits in a Large White pig population
    Shuji Sato
    National Livestock Breeding Center, Nishigo, Fukushima, Japan
    Anim Sci J 87:1455-1463. 2016
    ..Six candidate SNPs were associated with TNB; the only exception was a PRKAG3 polymorphism...
  21. doi PRKAG3 polymorphisms associated with sporadic Wolff-Parkinson-White syndrome among a Taiwanese population
    Ken Pen Weng
    Department of Pediatrics, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC Faculty of Medicine, National Yang Ming University, Taipei, Taiwan, ROC Electronic address
    J Chin Med Assoc 79:656-660. 2016
    The aim of this study was to investigate whether mutation in AMP-activated protein kinase (AMPK) subunit genes (PRKAG3-230) is associated with sporadic, isolated Wolff-Parkinson-White (WPW) syndrome.
  22. doi Two-hybrid analysis identifies PSMD11, a non-ATPase subunit of the proteasome, as a novel interaction partner of AMP-activated protein kinase
    Daniel Moreno
    Instituto de Biomedicina de Valencia, CSIC and CIBER de Enfermedades Raras CIBERER, Jaime Roig 11, 46010 Valencia, Spain
    Int J Biochem Cell Biol 41:2431-9. 2009
    ..Our results indicate that AMPK is able to interact physically with this subunit and modify its phosphorylation status, supporting a possible role for AMPK in regulating proteasome function...
  23. ncbi Molecular cloning and characterization of bovine PRKAG3 gene: structure, expression and single nucleotide polymorphism detection
    S L Yu
    Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Korea
    J Anim Breed Genet 122:294-301. 2005
    The protein kinase adenosine monophosphate-activated gamma3-subunit (PRKAG3) gene encodes a muscle-specific isoform of the regulatory gamma-subunit of adenosine monophosphate-activated protein kinase, which plays a key role in regulating ..
  24. doi Polymorphisms in AKT3, FIGF, PRKAG3, and TGF-β genes are associated with myofiber characteristics in chickens
    Sirui Chen
    National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
    Poult Sci 92:325-30. 2013
    ..vicinity of quantitative trait loci affecting breast muscle weight, including INS, IGF2, PIK3C2A, AKT3, PRKAB2, PRKAG3, VEGFA, RPS6KA2/3, FIGF, and TGF-β1/2/3, were chosen to be genotyped by high-throughput matrix-assisted laser ..
  25. doi PRKAG3 and CAST genetic polymorphisms and quality traits of dry-cured hams--III. Associations in Slovenian dry-cured ham Kraški pršut and their dependence on processing
    M Skrlep
    Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia
    Meat Sci 92:360-5. 2012
    The aim of the present study, the third in a series of three papers, is to show the effects of PRKAG3 and CAST gene polymorphisms on the quality traits of the Slovenian dry-cured ham "Kraški pršut" and their interaction with ham ..
  26. doi HDAC1/NFκB pathway is involved in curcumin inhibiting of Tat-mediated long terminal repeat transactivation
    Hong Sheng Zhang
    College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
    J Cell Physiol 226:3385-91. 2011
    ..Collectively, our data provide new insights into understanding of the molecular mechanisms of curcumin inhibited Tat-regulated transcription, suggesting that targeting AMPK/HDAC1/NFκB pathway could serve as new anti-HIV-1 agents...
  27. doi CARM1/PRMT4 is necessary for the glycogen gene expression programme in skeletal muscle cells
    Shu Ching Mary Wang
    Obesity Research Centre, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia
    Biochem J 444:323-31. 2012
    ..associated with decreased expression of the mRNAs encoding AMPK (AMP-activated protein kinase) α2/γ3 (Prkaa2 and Prkag3) and p38 MAPK (mitogen-activated protein kinase), previously implicated in Wolff-Parkinson-White syndrome and ..
  28. doi Evaluation of QTL for carcass merit and meat quality traits in a US commercial Duroc population
    Igseo Choi
    Department of Animal Science, Michigan State University, East Lansing, MI 48824 1225, USA
    Meat Sci 92:132-8. 2012
    ..The MDH1 SNP on SSC3 was associated with 45-min and ultimate pH (pHu), and pH decline. PRKAG3 on SSC15 was associated with pHu. The HSPG2 SNP on SSC6 was associated with marbling score and days to 113kg...
  29. ncbi The bovine 5' AMPK gene family: mapping and single nucleotide polymorphism detection
    Stephanie D McKay
    Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843 4467, USA
    Mamm Genome 14:853-8. 2003
    ..0. PRKAA1 mapped to BTA 20, PRKAA2 and PRKAB2 to BTA 3, PRKAB1 to BTA 17, PRKAG1 to BTA 5, PRKAG2 to BTA 4, and PRKAG3 to BTA 2. Five of the seven genes mapped to regions expected from human/cattle comparative maps...
  30. doi Association of single nucleotide polymorphism (SNP) markers in candidate genes and QTL regions with pork quality traits in commercial pigs
    G A Rohrer
    USDA, ARS, U S Meat Animal Research Center, Spur 18D, Clay Center, NE 68933 0166, USA
    Meat Sci 92:511-8. 2012
    ..Genes with the largest effects on pork quality were MC4R, IGF2, CAST and PRKAG3.
  31. doi PRKAG3 and CAST genetic polymorphisms and quality traits of dry-cured hams--I. Associations in Spanish dry-cured ham Jamón Serrano
    P Gou
    IRTA, Food Technology, XaRTA, Finca Camps i Armet, 17121 Monells, Spain
    Meat Sci 92:346-53. 2012
    ..slaughter, and the Ile199Val polymorphism identified in the coding region of the protein kinase AMP-activated (PRKAG3) gene has been proven to affect ultimate pH in muscle...
  32. doi Allelic frequencies of PRKAG3 in several pig breeds and its technological consequences on a Duroc × Landrace-Large White cross
    A Galve
    Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain
    J Anim Breed Genet 130:382-93. 2013
    The allelic frequencies of PRKAG3 gene (the RN gene) have been investigated in several pig breeds...
  33. doi SIRT1 regulates Tat-induced HIV-1 transactivation through activating AMP-activated protein kinase
    Hong Sheng Zhang
    Department of Virology and Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Pingleyuan 100, District of Chaoyang, Beijing 100124, China
    Virus Res 146:51-7. 2009
    ....
  34. pmc SNP variation in the promoter of the PRKAG3 gene and association with meat quality traits in pig
    Marion T Ryan
    School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
    BMC Genet 13:66. 2012
    The PRKAG3 gene encodes the γ3 subunit of adenosine monophosphate activated protein kinase (AMPK), a protein that plays a key role in energy metabolism in skeletal muscle...
  35. doi Genome-wide association of meat quality traits and tenderness in swine
    D J Nonneman
    USDA ARS, U S Meat Animal Research Center, Clay Center, NE 68933, USA
    J Anim Sci 91:4043-50. 2013
    ..Some of the other compelling candidate genes in regions associated with meat quality include CEBPA, SNAI1, and FAM132A for IMF, CAPN1 for SSF, GLUL for pH, and PRKAG3 and ITGB1 with cooking loss.
  36. pmc Identification of prognostic genes for recurrent risk prediction in triple negative breast cancer patients in Taiwan
    Lee H Chen
    Bioinformatics and Biostatistics Core, Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
    PLoS ONE 6:e28222. 2011
    ..from the combination of the initial filtered genes, we developed a prediction model based on the genes SLC22A23, PRKAG3, DPEP3, MORC2, GRB7, and FAM43A. The model had 91.7% accuracy, 81.8% sensitivity, and 94...
  37. ncbi Associations of DNA markers with meat quality traits in pigs with emphasis on drip loss
    G Otto
    Institute of Animal Breeding and Husbandry, Christian Albrechts University of Kiel, Hermann Rodewald Straße 6, D 24118 Kiel, Germany
    Meat Sci 75:185-95. 2007
    ..Genotypes on 12 DNA markers, including RYR1 and PRKAG3 I199V, were also obtained on all pigs to investigate the relationship between genetic markers and meat quality...
  38. pmc Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds
    Lu Sheng Huang
    Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, 330045, PR China
    Genet Sel Evol 36:481-6. 2004
    Four missense substitutions (T30N, G52S, V199I and R200Q) in the porcine PRKAG3 gene were considered as the likely candidate loci affecting meat quality...
  39. ncbi Investigation of candidate genes for glycolytic potential of porcine skeletal muscle: Association with meat quality and production traits in Italian Large White pigs
    L Fontanesi
    DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Via F lli Rosselli 107, 42100 Reggio Emilia, Italy
    Meat Sci 80:780-7. 2008
    ..Three mutations in the PRKAG3 gene (T30N, G52S and I199V) were investigated as well as single nucleotide polymorphisms in two other skeletal ..
  40. doi Analysis of acid-soluble glycogen in pork extracts of two PRKAG3 genotypes by 1H liquid-state NMR spectroscopy and biochemical methods
    Flemming H Larsen
    Department of Food Science, University of Copenhagen, Rolighedsvej 30, DK 1958 Frederiksberg C, Denmark
    J Agric Food Chem 59:11895-902. 2011
    ..Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN(-) and rn(+) genotype) were analyzed by both (1)H liquid-state NMR spectroscopy and a biochemical ..
  41. ncbi Association between polymorphism in bovine PRKAG3 gene and milk production traits
    I Kowalewska-Luczak
    Department of Genetics and Animal Breeding, Westpomeranian University of Technology, Szczecin 71466, Poland
    Genetika 47:1135-9. 2011
    The aim of the conducted study was to evaluate correlation between genotypes and PRKAG3 compound genotypes and milk production traits (yield of milk, milk fat and milk protein, and protein and fat content in milk)...
  42. ncbi Determination of PRKAG1 coding sequence and mapping of PRKAG1 and PRKAG2 relatively to porcine back fat thickness QTL
    O Demeure
    Laboratoire de Genetique Cellulaire, INRA, BP 27, 31326 Castanet Tolosan, France
    Anim Genet 35:123-5. 2004
    PRKAG1, PRKAG2 and PRKAG3 encode three isoforms of AMP-activated protein kinase gamma chain. A major effect on meat quality and a medium effect on back fat thickness of the RN- mutation in the PRKAG3 gene has previously been reported...
  43. doi Muscle glycogen resynthesis, signalling and metabolic responses following acute exercise in exercise-trained pigs carrying the PRKAG3 mutation
    Birgitta Essen-Gustavsson
    Section for Comparative Physiology and Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, PO Box 7018, SE 750 07 Uppsala, Sweden
    Exp Physiol 96:927-37. 2011
    Hampshire pigs carrying the PRKAG3 mutation in the AMP-activated protein kinase (AMPK) γ3 subunit exhibit excessive skeletal muscle glycogen storage and an altered glycogen synthesis signalling response following exercise...
  44. ncbi Molecular characterization and mutational screening of the PRKAG3 gene in the horse
    H B Park
    Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Cytogenet Genome Res 102:211-6. 2003
    The PRKAG3 gene encodes a muscle-specific isoform of the regulatory gamma subunit of AMP-activated protein kinase (AMPK)...
  45. ncbi Comparative sequence analysis of the PRKAG3 region between human and pig: evolution of repetitive sequences and potential new exons
    V Amarger
    Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Cytogenet Genome Res 102:163-72. 2003
    The PRKAG3 gene encodes the gamma3 chain of AMP-activated protein kinase (AMPK). A non-conservative missense mutation in the PRKAG3 gene causes a dominant phenotype involving abnormally high glycogen content in pig skeletal muscle...
  46. ncbi Study of candidate genes for glycolytic potential of porcine skeletal muscle: identification and analysis of mutations, linkage and physical mapping and association with meat quality traits in pigs
    L Fontanesi
    DIPROVAL Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Reggio Emilia, Italy
    Cytogenet Genome Res 102:145-51. 2003
    Several genes (PRKAA2, PRKAB1, PRKAB2, PRKAG3, GAA, GYS1, PYGM, ALDOA, GPI, LDHA, PGAM2 and PKM2), chosen according to their role in the regulation of the energy balance and in the glycogen metabolism and glycolysis of the skeletal muscle,..
  47. ncbi TSC2 mediates cellular energy response to control cell growth and survival
    Ken Inoki
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Cell 115:577-90. 2003
    ..These observations demonstrate a model where TSC2 functions as a key player in regulation of the common mTOR pathway of protein synthesis, cell growth, and viability in response to cellular energy levels...
  48. ncbi [A porcine linkage map of microsatellite markers using a commercial population as a reference]
    Xiao ling Guo
    College of Animal Science, Zhejiang University, Hangzhou 310029, China
    Yi Chuan Xue Bao 30:653-6. 2003
    ..The genetic markers used in this study include 172 microsatellite markers and 3 type- I markers (RYR1, PIT1 and PRKAG3). All microsatellite markers were genotyped using multiplex PCR reactions and visualized on ABI 377 sequencer...
  49. ncbi A large-insert porcine library with sevenfold genome coverage: a tool for positional cloning of candidate genes for major quantitative traits
    Jin Tae Jeon
    Division of Animal Science, College of Agriculture, Gyeongsang National University, Jinju 660 701, Korea
    Mol Cells 16:113-6. 2003
    ..The library is being used to isolate specific clones by screening with type I and type II marker clones located in the QTL region affecting intramuscular fat content on SSC6...
  50. ncbi Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases
    Barbara Burwinkel
    Institut fur Physiologische Chemie, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Eur J Hum Genet 11:516-26. 2003
    ..of glycogen phosphorylase (PYGM), of a muscle-specific regulatory subunit of the AMP-dependent protein kinase (PRKAG3), and the promoter regions of PHKA1, PHKB and PHKG1...
  51. ncbi Identification and characterization of AMPK gamma 3 mutations in the pig
    L Andersson
    Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, S 751 24 Uppsala, Sweden
    Biochem Soc Trans 31:232-5. 2003
    ..The protein kinase AMP-activated gamma 3 subunit gene, PRKAG3, which encodes the gamma 3 isoform of AMP-activated protein kinase (AMPK), was identified as the causative gene ..
  52. pmc Evidence for new alleles in the protein kinase adenosine monophosphate-activated gamma(3)-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality
    D Ciobanu
    Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
    Genetics 159:1151-62. 2001
    ..On the basis of the QTL location the PRKAG3 (protein kinase, AMP-activated, gamma(3)-subunit) gene was considered to be a good candidate for the observed ..
  53. ncbi Comparative analysis of a BAC contig of the porcine RN region and the human transcript map: implications for the cloning of trait loci
    J T Jeon
    Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, S 751 24, Sweden
    Genomics 72:297-303. 2001
    ..The contig was constructed as part of a successful positional cloning project, which identified PRKAG3 as the causative gene for the RN phenotype...
  54. ncbi Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro
    A Woods
    Medical Research Council Clinical Sciences Centre, Department of Molecular Medicine, Royal Postgraduate Medical School, DuCane Road, London W12 0NN, United Kingdom
    J Biol Chem 271:10282-90. 1996
    ..These results suggest that AMPKbeta mediates the association of the heterotrimeric AMPK complex in vitro, and will facilitate future studies aimed at investigating the regulation of AMPK in vivo...
  55. doi Relationship between AMPK and the transcriptional balance of clock-related genes in skeletal muscle
    Elaine Vieira
    Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Am J Physiol Endocrinol Metab 295:E1032-7. 2008
    ..AMPKgamma3 knockout (Prkag3(-/-)) mice or wild-type littermates were injected with saline or an AMPK activator, 5-amino-4-imidazole-..
  56. ncbi 5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics
    Brian R Barnes
    Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
    FASEB J 19:773-9. 2005
    ..We used AMPK3 transgenic (Tg-Prkag3225Q) and knockout (Prkag3-/-) mice to determine the relationship between AMPK activity, glycogen content, and ergogenics (ability to perform ..
  57. ncbi [QTL mapping for ear shape based on a commercial pig population]
    Xiao ling Guo
    College of Animal Science, Zhejiang University, Hangzhou 310029, China
    Yi Chuan Xue Bao 31:819-21. 2004
    ..which a linkage map of whole genome was created using 172 microsatellite markers and three type-1 markers (RYR1, PRKAG3, PIT1)...
  58. ncbi Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle
    Y C Long
    Department of Surgical Sciences, Section for Integrative Physiology, Karolinska Institute, Stockholm, Sweden
    Diabetologia 48:2354-64. 2005
    ..The aim of this study was to investigate whether AMPK modulates the expression of skeletal muscle genes that have been implicated in lipid and glucose metabolism under fed or fasting conditions...
  59. ncbi UDP-glucose pyrophosphorylase is upregulated in carriers of the porcine RN- mutation in the AMP-activated protein kinase
    Jakob Hedegaard
    The Department of Food Sciences, Danish Institute of Agricultural Sciences, Tjele, Denmark
    Proteomics 4:2448-54. 2004
    ..A dominant mutation, denoted RN(-), in the porcine PRKAG3 gene, encoding the regulatory gamma3 subunit of AMPK, results in hyperaccumulation of glycogen in glycolytic ..
  60. ncbi Opposite transcriptional regulation in skeletal muscle of AMP-activated protein kinase gamma3 R225Q transgenic versus knock-out mice
    Elisabeth C Nilsson
    Arexis AB, Biotech Center, Arvid Wallgrens Backe 20, SE 413 46 Goteborg, Sweden
    J Biol Chem 281:7244-52. 2006
    ..The muscle-specific isoform of the regulatory gamma-subunit of the kinase, AMPK gamma3, has an important role in glucose uptake, glycogen synthesis, and fat oxidation in white skeletal muscle, as ..
  61. ncbi Characterization of the bovine PRKAG3 gene: structure, polymorphism, and alternative transcripts
    Matthieu Roux
    Faculte des Sciences et Techniques, Unité de Génétique Moléculaire Animale, Unité Mixte de Recherches 1061 Institut national de la recherche agronomique INRA Université de Limoges, 123 av Albert Thomas, 87060 Limoges Cedex, France
    Mamm Genome 17:83-92. 2006
    The bovine PRKAG3 gene encodes the AMPK gamma3 subunit, one isoform of the regulatory gamma subunit of the AMP-activated protein kinase (AMPK)...
  62. ncbi A genome scan for loci affecting pork quality in a Duroc-Landrace F population
    G A Rohrer
    USDA, ARS, U S Meat Animal Research Center, Clay Center, NE, USA
    Anim Genet 37:17-27. 2006
    ..Fixed effects included sex and age at slaughter. For certain analyses carcass weight, RYR1 genotype and/or PRKAG3 genotype were also included as covariates...
  63. ncbi Identification of single-nucleotide polymorphisms in 5' end and exons of the PRKAG3 gene in Hubbard White broiler, Leghorn layer, and three Chinese indigenous chicken breeds
    C J Zhao
    Key Laboratories of Animal Breeding and Genetics of Ministry of Agriculture of P R China
    J Anim Breed Genet 123:349-52. 2006
    ..Previous studies revealed that polymorphisms in 5'-AMP-activated protein kinase gamma 3 subunit (PRKAG3) gene are associated with meat quality in pigs...
  64. ncbi Adenosine 5'-monophosphate kinase-activated protein kinase (PRKA) activators delay meiotic resumption in porcine oocytes
    Mario A Mayes
    Centre de Recherche en Biologie de la Reproduction, Departement des Sciences Animales, Faculté des Sciences de l Agriculture et d Alimentation, Universite Laval, Quebec, Canada G1K 7P4
    Biol Reprod 76:589-97. 2007
    ..The PRKAA2 subunit was detected in granulosa cells and COC, whereas the PRKAG3 subunit was not detected in granulosa cells, COC or DO, whereas it was detected in the heart...
  65. ncbi Regulation of the muscle-specific AMP-activated protein kinase alpha2beta2gamma3 complexes by AMP and implications of the mutations in the gamma3-subunit for the AMP dependence of the enzyme
    Kerstin Lindgren
    Discovery Research, Biovitrum AB, Biotech Center, Arvid Wallgrens Backe 20, SE 413 46 Goteborg, Sweden
    FEBS J 274:2887-96. 2007
    ....
  66. ncbi Genomic imbalances in Schistosoma-associated and non-Schistosoma-associated bladder carcinoma. An array comparative genomic hybridization analysis
    Gemma Armengol
    U Antropologia, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Faculty of Sciences, Universitat Autonoma de Barcelona, Bellaterra, Barcelona 08193, Spain
    Cancer Genet Cytogenet 177:16-9. 2007
    ..23) and some lost (PRKAG3 on 2q35 and PRDM6 on 5q23.2) in SA-SCC...
  67. ncbi Role of AMP-activated protein kinase gamma 3 genetic variability in glucose and lipid metabolism in non-diabetic whites
    P Weyrich
    Department of Internal Medicine, Division of Endocrinology, Metabolism, Pathobiochemistry and Clinical Chemistry, University of Tubingen, Otfried Muller Str 10, 72076, Tubingen, Germany
    Diabetologia 50:2097-106. 2007
    ..The AMPKgamma3 (protein kinase, AMP-activated, gamma 3 non-catalytic subunit, PRKAG3) isoform plays a role in gene regulation in glucose/lipid metabolism and skeletal muscle glycogen content...
  68. pmc Gain-of-function R225W mutation in human AMPKgamma(3) causing increased glycogen and decreased triglyceride in skeletal muscle
    Sheila R Costford
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
    PLoS ONE 2:e903. 2007
    ..Here we report the first gain-of-function mutation in the muscle-specific regulatory gamma(3) subunit in humans...
  69. pmc AMPK phosphorylation of raptor mediates a metabolic checkpoint
    Dana M Gwinn
    Molecular and Cell Biology Laboratory, Dulbecco Center for Cancer Research, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Mol Cell 30:214-26. 2008
    ..These findings uncover a conserved effector of AMPK that mediates its role as a metabolic checkpoint coordinating cell growth with energy status...
  70. ncbi The 5'-AMP-activated protein kinase gamma3 isoform has a key role in carbohydrate and lipid metabolism in glycolytic skeletal muscle
    Brian R Barnes
    Department of Surgical Sciences and the Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
    J Biol Chem 279:38441-7. 2004
    ..To determine the functional role of the AMPK gamma3 isoform, we generated transgenic mice with skeletal muscle-specific expression of wild type or mutant (225Q) ..
  71. ncbi Microarray of SNPs for diverse applications in commercial pig breeding
    S Kamińiski
    University of Warmia and Mazury, Department of Animal Genetics, 10 718 Olsztyn, Oczapowskiego 5, Poland
    Pol J Vet Sci 12:69-74. 2009
    ..Polymorphisms) which enables the determining of 1 genetic disease (PSS-Porcine Stress Syndrome), 4 QTLs genes: PRKAG3, CAST, MC4R and ESR, which together with the remaining SNPs create a panel useful in marker-assisted selection and ..
  72. pmc Association study of AMP-activated protein kinase subunit genes in polycystic ovary syndrome
    Kari Sproul
    Department of Obstetrics and Gynecology, Cedars Sinai Medical Center, Los Angeles, California 90048, USA
    Eur J Endocrinol 161:405-9. 2009
    To examine the genes for AMP-activated protein kinase (AMPK) subunits alpha2 (PRKAA2) and gamma3 (PRKAG3) as candidates for polycystic ovary syndrome (PCOS) and its component traits.
  73. doi Purification and characterization of truncated human AMPK alpha 2 beta 2 gamma 3 heterotrimer from baculovirus-infected insect cells
    Lata Ramanathan
    Protein Science Department, Schering Plough Research Institute, 320 Bent St, Cambridge, MA 02141, USA
    Protein Expr Purif 70:13-22. 2010
    ..The gamma 1 and gamma 3 isoforms showed comparable nucleotide binding affinities and solution behavior properties...
  74. doi Effects of exercise on muscle glycogen synthesis signalling and enzyme activities in pigs carrying the PRKAG3 mutation
    Anna Granlund
    Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, SE 750 07 Uppsala, Sweden
    Exp Physiol 95:541-9. 2010
    ..The mutation is situated in the PRKAG3 gene, which encodes a muscle-specific isoform of the AMP-activated protein kinase (AMPK) gamma3 subunit...
  75. doi Naturally occurring R225W mutation of the gene encoding AMP-activated protein kinase (AMPK)gamma(3) results in increased oxidative capacity and glucose uptake in human primary myotubes
    S A Crawford
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada
    Diabetologia 53:1986-97. 2010
    ..sought to characterise for the first time the effects of chronic AMPK activation on skeletal muscle carbohydrate metabolism in carriers of the rare gain-of-function mutation of the gene encoding AMPKgamma(3) subunit, PRKAG3 R225W.
  76. doi Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus
    W Reardon
    Teagasc, Ashtown Food Research Centre, Ashtown, Dublin 15, Ireland
    Meat Sci 86:270-5. 2010
    ..However, SNP in CAST, amp-activated protein kinase, gamma-3 subunit (PRKAG3), growth hormone receptor (GHR) and stearoyl coA desaturase (SCD) genes were significantly associated with colour ..
  77. doi Association study of energy homeostasis genes and antipsychotic-induced weight gain in patients with schizophrenia
    G Jassim
    Department of Clinical Medicine, University of Bergen, Bergen, Norway
    Pharmacopsychiatry 44:15-20. 2011
    ..Such adverse effects have been proposed to occur through drug-induced mechanisms involving both the central nervous system and different peripheral tissues...
  78. doi Updated estimates of HAL n and RN- effects on pork quality: fresh and processed loin and ham
    P Cherel
    FRANCE HYBRIDES, 100 Avenue Denis Papin, F 45800 Saint Jean de Braye, France
    Meat Sci 86:949-54. 2010
    ..Using the same methodology, significant effects of the PRKAG3 (RN) I199 allele on ultimate pH values but not on glycolytic potential were observed.
  79. doi Proteomic profile of dry-cured ham relative to PRKAG3 or CAST genotype, level of salt and pastiness
    Martin Skrlep
    Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia
    Meat Sci 88:657-67. 2011
    ..The PRKAG3 affected mainly muscle metabolic enzymes, indicating its possible influence on muscle metabolism with ..
  80. pmc The influence of the PRKAG3 mutation on glycogen, enzyme activities and fibre types in different skeletal muscles of exercise trained pigs
    Anna Granlund
    Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, SE 750 07, Uppsala, Sweden
    Acta Vet Scand 53:20. 2011
    ..Pigs carrying this PRKAG3 (R225Q) mutation have, compared to non-carriers, higher muscle glycogen levels and increased oxidative capacity in ..
  81. doi Validation of a paternally imprinted QTL affecting pH24h distinct from PRKAG3 on SSC15
    R M Qiao
    Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, 330045, China
    Anim Genet 42:316-20. 2011
    ..and it was also unclear whether the observed QTL effect was completely or partially caused by mutations in the PRKAG3 gene, which is the only major gene on SSC15 so far known to influence pH24h...
  82. ncbi [Comparison between the female- and male-linkage maps in pigs]
    Xiao ling Guo
    College of Animal Science, Zhejiang University, Hangzhou 310 029, China
    Yi Chuan 26:644-6. 2004
    ..based on a commercial porcine population and using 163 microsatellite markers as well as 3 type-I markers (RYR1, PRKAG3, PIT1), was statistic analyzed...
  83. pmc Joint analysis of quantitative trait loci and major-effect causative mutations affecting meat quality and carcass composition traits in pigs
    Pierre Cherel
    INRA, UMR0598, Génétique Animale, 35042 Rennes Cedex, France
    BMC Genet 12:76. 2011
    ..The RYR1 and PRKAG3 causative mutations, originally identified from major effects on meat characteristics, can be used both as ..

Research Grants3

  1. FASEB Research Conf: Glucose Transporter Biology
    Barbara Kahn; Fiscal Year: 2003
    ..abstract_text> ..
  2. GLUCOSE TRANSPORTER REGULATION IN OBESITY AND DIABETES
    Barbara Kahn; Fiscal Year: 2005
    ..Our goal is to find new therapeutic targets to prevent or ameliorate type 2 diabetes. ..
  3. Protein Tyrosine Phosphatase 1B and Insulin Action
    Barbara Kahn; Fiscal Year: 2005
    ..Our goal is to find new therapeutic targets to reduce insulin resistance and prevent or ameliorate type 2 diabetes. ..