PPM1G

Summary

Gene Symbol: PPM1G
Description: protein phosphatase, Mg2+/Mn2+ dependent 1G
Alias: PP2CG, PP2CGAMMA, PPP2CG, protein phosphatase 1G, PP2C-gamma, protein phosphatase 1C, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform, protein phosphatase 2, catalytic subunit, gamma isoform, protein phosphatase 2C gamma isoform, protein phosphatase 2C isoform gamma, protein phosphatase magnesium-dependent 1 gamma
Species: human

Top Publications

  1. ncbi Crystal structures of protein phosphatase-1 bound to motuporin and dihydromicrocystin-LA: elucidation of the mechanism of enzyme inhibition by cyanobacterial toxins
    Jason T Maynes
    Canadian Institutes of Health Research, Group in Protein Structure and Function Department of Biochemistry, Faculty of Medicine, University of Alberta, Edmonton, Alta, Canada T6G 2H7
    J Mol Biol 356:111-20. 2006
  2. pmc Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex
    Wen Luo
    Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112 5550, USA
    EMBO J 26:1511-21. 2007
  3. doi Loss of protein phosphatase 1c{gamma} (PPP1CC) leads to impaired spermatogenesis associated with defects in chromatin condensation and acrosome development: an ultrastructural analysis
    Nicole Forgione
    Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada M5S 3G5
    Reproduction 139:1021-9. 2010
  4. pmc FIN13, a novel growth factor-inducible serine-threonine phosphatase which can inhibit cell cycle progression
    M A Guthridge
    Department of Microbiology and Kaplan Cancer Center, New York University School of Medicine, New York 10016, USA
    Mol Cell Biol 17:5485-98. 1997
  5. ncbi PP2C gamma: a human protein phosphatase with a unique acidic domain
    S M Travis
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
    FEBS Lett 412:415-9. 1997
  6. pmc ATM-dependent downregulation of USP7/HAUSP by PPM1G activates p53 response to DNA damage
    Svetlana V Khoronenkova
    Department of Oncology, Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford OX3 7DQ, UK
    Mol Cell 45:801-13. 2012
  7. pmc The type 2C Ser/Thr phosphatase PP2Cgamma is a pre-mRNA splicing factor
    M V Murray
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 USA
    Genes Dev 13:87-97. 1999
  8. pmc Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2Cgamma governs Cajal body localization and stability of the SMN complex
    Sebastian Petri
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, 69120 Heidelberg, Germany
    J Cell Biol 179:451-65. 2007
  9. ncbi Alternative splicing regulation by interaction of phosphatase PP2Cgamma with nucleic acid-binding protein YB-1
    Eric Allemand
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Struct Mol Biol 14:630-8. 2007
  10. ncbi PP2Cgamma-mediated S-phase accumulation induced by the proteasome-dependent degradation of p21(WAF1/CIP1)
    Eun Jung Suh
    Department of Biology, Kyung Hee University, Republic of Korea
    FEBS Lett 580:6100-4. 2006

Research Grants

  1. Danielle M Dick; Fiscal Year: 2016
  2. Sodium Channel Blockers as Inhibitors of Prostate Cancer
    Milton Brown; Fiscal Year: 2008

Scientific Experts

  • Milton Brown
  • Ralf Enz
  • Sergio Papa
  • S M Travis
  • Kazuyuki Nakamura
  • Hiroshi Kimura
  • Danielle M Dick
  • Swapna Aravind Gudipaty
  • Iván D'Orso
  • Ryan P McNamara
  • Tamara D Skene-Arnold
  • Eun Jung Suh
  • Alma Husedzinovic
  • Charles F B Holmes
  • Oliver J Gruss
  • Sophia M LeMaire
  • K Xu
  • Madalina Raducu
  • Tandekile Lubelwana Hafver
  • Chuang Sun
  • Thibaut Barnoud
  • Yasunori Sugiyama
  • Barbara Ruggeri
  • Vickie Hannig
  • Bernard P C Kok
  • Neelam Chaudhary
  • Benedikt M Kessler
  • William H Foster
  • Jianyu Liu
  • Chang Pan
  • Xiang Li
  • C Manser
  • David M Perry
  • Svetlana V Khoronenkova
  • Catherine Manser
  • Petra Beli
  • Liang Sun
  • H Henderson
  • Utz Fischer
  • Scott Grindrod
  • Martin Voss
  • Elizabeth Scotto-Lavino
  • Nicole Forgione
  • Michael N G James
  • Meritxell Gironella
  • Scoty M Hearst
  • Susannah Varmuza
  • Igor Tchivilev
  • Kyu Heo
  • Sang Hoon Kim
  • Peter Rellos
  • Eric Allemand
  • Sebastian Petri
  • Christopher Hrabchak
  • Ana Marija Hristovska
  • Patrice Arthur
  • Wen Luo
  • Jiayi Zhang
  • Jason T Maynes
  • Ivan Munoz
  • S Varmuza
  • Cameron R Grady
  • Mark A Knepper
  • Chung Lin Chou
  • Viswanathan Raghuram
  • Ezigbobiara N Umejiego
  • Christina M Pickering
  • Isamu Kameshita
  • John C Christianson
  • Noriyuki Sueyoshi
  • Per Kristian Lunde
  • Gaohang Wang
  • Katharine H Wrighton
  • Vincenzo D'Angiolella
  • Marianne Lunde
  • Marc Flajolet
  • Naoki Goshima
  • Syouichi Katayama
  • Sho Yamashita
  • Sébastien Serres
  • Bjørn Dalhus
  • Howard Donninger
  • W Feng
  • Lucia Di Marcotullio
  • Francesca M Buffa
  • Rune Toftgard
  • Nicola R Sibson
  • Jan Magnus Aronsen
  • H Kg Shu
  • Marie Laetitia Thézenas

Detail Information

Publications60

  1. ncbi Crystal structures of protein phosphatase-1 bound to motuporin and dihydromicrocystin-LA: elucidation of the mechanism of enzyme inhibition by cyanobacterial toxins
    Jason T Maynes
    Canadian Institutes of Health Research, Group in Protein Structure and Function Department of Biochemistry, Faculty of Medicine, University of Alberta, Edmonton, Alta, Canada T6G 2H7
    J Mol Biol 356:111-20. 2006
    ....
  2. pmc Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex
    Wen Luo
    Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112 5550, USA
    EMBO J 26:1511-21. 2007
    ..Specific inhibition of PP1 in this pathway may offer therapeutic approaches to disorders with increased beta-catenin signaling...
  3. doi Loss of protein phosphatase 1c{gamma} (PPP1CC) leads to impaired spermatogenesis associated with defects in chromatin condensation and acrosome development: an ultrastructural analysis
    Nicole Forgione
    Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada M5S 3G5
    Reproduction 139:1021-9. 2010
    ..Electron microscopy revealed major acrosomal and chromatin condensation defects in Ppp1cc mutants. Our observations are discussed in the context of known molecular changes in Ppp1cc mutant testes...
  4. pmc FIN13, a novel growth factor-inducible serine-threonine phosphatase which can inhibit cell cycle progression
    M A Guthridge
    Department of Microbiology and Kaplan Cancer Center, New York University School of Medicine, New York 10016, USA
    Mol Cell Biol 17:5485-98. 1997
    ....
  5. ncbi PP2C gamma: a human protein phosphatase with a unique acidic domain
    S M Travis
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
    FEBS Lett 412:415-9. 1997
    ..It is 34% identical to mammalian PP2C alpha and PP2C beta and we call it PP2C gamma. It more closely resembles PP2Cs from Paramecium tetraurelia and Schizosaccharomyces pombe than mammalian PP2Cs...
  6. pmc ATM-dependent downregulation of USP7/HAUSP by PPM1G activates p53 response to DNA damage
    Svetlana V Khoronenkova
    Department of Oncology, Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford OX3 7DQ, UK
    Mol Cell 45:801-13. 2012
    ..After ionizing radiation, dephosphorylation of USP7S by the ATM-dependent protein phosphatase PPM1G leads to USP7S downregulation, followed by Mdm2 downregulation and accumulation of p53...
  7. pmc The type 2C Ser/Thr phosphatase PP2Cgamma is a pre-mRNA splicing factor
    M V Murray
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 USA
    Genes Dev 13:87-97. 1999
    ..A component of SCF1 is shown to be PP2Cgamma, a type 2C Ser/Thr phosphatase of previously unknown function...
  8. pmc Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2Cgamma governs Cajal body localization and stability of the SMN complex
    Sebastian Petri
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, 69120 Heidelberg, Germany
    J Cell Biol 179:451-65. 2007
    ..Here, we show that the nuclear phosphatase PPM1G/PP2Cgamma interacts with and dephosphorylates the SMN complex...
  9. ncbi Alternative splicing regulation by interaction of phosphatase PP2Cgamma with nucleic acid-binding protein YB-1
    Eric Allemand
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Struct Mol Biol 14:630-8. 2007
    ..The human Ser/Thr phosphatase PP2Cgamma promotes spliceosome assembly...
  10. ncbi PP2Cgamma-mediated S-phase accumulation induced by the proteasome-dependent degradation of p21(WAF1/CIP1)
    Eun Jung Suh
    Department of Biology, Kyung Hee University, Republic of Korea
    FEBS Lett 580:6100-4. 2006
    ..However, the function of PP2Cgamma in cell cycle progression is still unclear...
  11. pmc A novel histone exchange factor, protein phosphatase 2Cgamma, mediates the exchange and dephosphorylation of H2A-H2B
    Hiroshi Kimura
    Nuclear Function and Dynamics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 175:389-400. 2006
    ..factors that assist in the incorporation of H2A-H2B, we identified protein phosphatase (PP) 2C gamma subtype (PP2Cgamma/PPM1G) as a histone chaperone that binds to and dephosphorylates H2A-H2B...
  12. pmc WWP2-WWP1 ubiquitin ligase complex coordinated by PPM1G maintains the balance between cellular p73 and ΔNp73 levels
    Neelam Chaudhary
    Laboratory of Cell Death and Cell Survival, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad, India
    Mol Cell Biol 34:3754-64. 2014
    ..Further, we identified phosphatase PPM1G as a functional switch that controls the balance between monomeric WWP2 and a WWP2/WWP1 heterodimeric state in the ..
  13. doi Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins
    Liang Sun
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1823:1092-101. 2012
    ..The proteins identified suggest that Aha1 may play roles in modulating RNA splicing and DNA repair, in addition to other cellular processes...
  14. doi Ppm1E is an in cellulo AMP-activated protein kinase phosphatase
    Martin Voss
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland, UK
    Cell Signal 23:114-24. 2011
    ..The data indicate that Ppm1E and probably PpM1F are in cellulo AMPK phosphatases and that Ppm1E is a potential anti-diabetic drug target...
  15. doi p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage
    Meritxell Gironella
    INSERM U 624, Stress Cellulaire, Case 915, Parc Scientifique et Technologique de Luminy, Marseille Cedex 9, France
    J Cell Physiol 221:594-602. 2009
    ..MSL1-dependent HAT activity, which is negatively regulated by the stress protein p8, induces chromatin remodeling and relaxation allowing access to DNA of the repair machinery...
  16. pmc Conserved residues in the N terminus of lipin-1 are required for binding to protein phosphatase-1c, nuclear translocation, and phosphatidate phosphatase activity
    Bernard P C Kok
    From the Signal Transduction Research Group, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S2, Canada and
    J Biol Chem 289:10876-86. 2014
    ..In conclusion, we provide novel evidence of the importance of the lipin-1 N-terminal domain for its catalytic activity, nuclear localization, and binding to PP-1cγ. ..
  17. pmc A Novel SHOC2 Variant in Rasopathy
    Vickie Hannig
    Division of Medical Genetics and Genomic Medicine, Vanderbilt University, Nashville, Tennessee
    Hum Mutat 35:1290-4. 2014
    ..significance of this SHOC2 variant revealed that the mutant protein has impaired capacity to interact with protein phosphatase 1c (PP1c), leading to insufficient activation of RAF-1 kinase...
  18. pmc The catalytically inactive tyrosine phosphatase HD-PTP/PTPN23 is a novel regulator of SMN complex localization
    Alma Husedzinovic
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Universitat Heidelberg, D 69120 Heidelberg, Germany
    Mol Biol Cell 26:161-71. 2015
    ..Several phosphatases had been implicated in the regulation of SMN, including the nuclear phosphatases PPM1G and PP1γ...
  19. pmc Association of Protein Phosphatase PPM1G With Alcohol Use Disorder and Brain Activity During Behavioral Control in a Genome-Wide Methylation Analysis
    Barbara Ruggeri
    From the Institute of Psychiatry, King s College London the MRC Social, Genetic, and Developmental Psychiatry Centre, London the Department of Public Health, the Department of Mental Health and Substance Abuse Services, and The Institute for Molecular Medicine, University of Helsinki, Helsinki the National Institute for Health and Welfare, Helsinki the Central Institute of Mental Health, Medical Faculty Mannheim, the Department of Addictive Behaviour and Addiction Medicine, and the Department of Cognitive and Clinical Neuroscience, Heidelberg University, Mannheim, Germany the Institute of Neuroscience, Trinity College Dublin, Dublin, Ireland Universitaetsklinikum Hamburg Eppendorf, Hamburg, Germany the Department of Psychiatry, University of Montreal, and CHU Sainte Justine Hospital, Montreal NeuroSpin, Alternative Energies and Atomic Energy Commission CEA, Paris the Department of Psychiatry and Psychotherapy, Campus Charite Mitte, Charite Universitatsmedizin Berlin, Germany the Departments of Psychiatry and Psychology, University of Vermont, Middlebury the School of Physics and Astronomy and the School of Psychology, University of Nottingham, Nottingham, United Kingdom Physikalisch Technische Bundesanstalt, Braunschweig und Berlin, Germany Institut National de la Santé et de la Recherche Médicale, CHRU de Nancy
    Am J Psychiatry 172:543-52. 2015
    ..Despite growing evidence associating epigenetics and psychiatric disorders, it is unclear how epigenetics, particularly DNA methylation, relate to brain function and behavior, including drinking behavior...
  20. pmc SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development
    Madalina Raducu
    Cancer Research UK and Medical Research Council Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, UK
    EMBO J 35:1400-16. 2016
    ..In summary, our findings reveal Fbxl17 as a novel regulator of Hh pathway and highlight the perturbation of the Fbxl17-Sufu axis in the pathogenesis of medulloblastoma. ..
  21. pmc Regulation of p27Kip1 phosphorylation and G1 cell cycle progression by protein phosphatase PPM1G
    Chuang Sun
    Department of Molecular Physiology and Biophysics, Baylor College of MedicineHouston, TX 77030, USA Michael E DeBakey Department of Surgery, Baylor College of MedicineHouston, TX 77030, USA
    Am J Cancer Res 6:2207-2220. 2016
    ..By using genomic phosphatase screening, we identified a PPM family phosphatase, PPM1G, which could reduce p27 phosphorylation at T198...
  22. pmc Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct
    Sophia M LeMaire
    Epithelial Systems Biology Laboratory, Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland and
    Am J Physiol Renal Physiol 312:F84-F95. 2017
    ..gene symbols corresponding to the top-ranked phosphatases (common names in parentheses) were: Ppp1cb (PP1-β), Ppm1g (PP2C), Ppp1ca (PP1-α), Ppp3ca (PP2-B or calcineurin), Ppp2ca (PP2A-α), Ppp1cc (PP1-γ), Ppp2cb (PP2A-β), Ppp6c ..
  23. doi Phosphorylated TandeMBP: A unique protein substrate for protein phosphatase assay
    Yasunori Sugiyama
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761 0795, Japan Electronic address
    Anal Biochem 513:47-53. 2016
    ..Moreover, P-TandeMBP served as an efficient substrate for PPM family phosphatases (PPM1A, PPM1B, PPM1D, PPM1F, PPM1G, PPM1H, PPM1K, and PPM1M) and PPP family phosphatase PP5...
  24. pmc PPM1G Binds 7SK RNA and Hexim1 To Block P-TEFb Assembly into the 7SK snRNP and Sustain Transcription Elongation
    Swapna Aravind Gudipaty
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Cell Biol 35:3810-28. 2015
    ..Here we report that the PPM1G phosphatase (inducibly recruited by nuclear factor κB [NF-κB] to target promoters) directly binds 7SK RNA and ..
  25. pmc Functional interplay between PPM1G and the transcription elongation machinery
    Swapna Aravind Gudipaty
    Huntsman Cancer Institute, Salt Lake City, UT 84112, USA
    RNA Dis 3:. 2016
    ..Here, we highlight recent findings signifying the role of the PPM1G/PP2Cγ phosphatase in activating and maintaining the active transcription elongation state by regulating the ..
  26. pmc Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3
    Marc Flajolet
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:16006-11. 2003
    ..However, phosphorylation of the mGluR3 cytoplasmic tail at Ser-845 inhibits the interaction with PP2C. These results indicate distinct functions for mGluR2 and mGluR3 and suggest a dynamic regulation of mGluR3 by PP2C...
  27. pmc Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase
    K Xu
    Department of Radiation Oncology and the Winship Cancer Institute, Emory University, Atlanta, GA, USA
    Oncogene 35:5807-5816. 2016
    ..We now identify a potential role for the serine-threonine phosphatase PPM1G in translational regulation of Id1 protein expression...
  28. pmc Proteomics Analysis Reveals Novel RASSF2 Interaction Partners
    Thibaut Barnoud
    Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, KY 40202, USA
    Cancers (Basel) 8:. 2016
    ..We identified several proteins, including C1QBP, Vimentin, Protein phosphatase 1G and Ribonuclease inhibitor that function in diverse biological processes, including protein post-..
  29. pmc Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman
    Tandekile Lubelwana Hafver
    From the Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway, the KG Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, 0316 Oslo, Norway
    J Biol Chem 291:4561-79. 2016
    ..In conclusion, NCX1 is a substrate-specifying PP1c regulator protein, indirectly regulating NCX1 activity through pSer-68-PLM dephosphorylation. ..
  30. pmc Transcription factors mediate the enzymatic disassembly of promoter-bound 7SK snRNP to locally recruit P-TEFb for transcription elongation
    Ryan P McNamara
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9048, USA
    Cell Rep 5:1256-68. 2013
    ..We demonstrate that Tat recruits PPM1G/PP2Cγ to locally disassemble P-TEFb from the 7SK snRNP at the HIV promoter via dephosphorylation of the kinase T ..
  31. pmc Protein phosphatase PPM1G regulates protein translation and cell growth by dephosphorylating 4E binding protein 1 (4E-BP1)
    Jianyu Liu
    Markey Cancer Center, University of Kentucky, Lexington, Kentucky 40536 0509, USA
    J Biol Chem 288:23225-33. 2013
    ..Here, we report the identification of PPM1G as the phosphatase of 4E-BP1...
  32. pmc Nuclear phosphatase PPM1G in cellular survival and neural development
    William H Foster
    Molecular, Cellular and Integrated Physiology Program, University of California at Los Angeles, Los Angeles, California, USA
    Dev Dyn 242:1101-9. 2013
    b>PPM1G is a nuclear localized serine/threonine phosphatase implicated to be a regulator of chromatin remodeling, mRNA splicing, and DNA damage. However, its in vivo function is unknown.
  33. pmc Cadmium is a potent inhibitor of PPM phosphatases and targets the M1 binding site
    Chang Pan
    Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, Shandong University, School of Medicine, Jinan, Shandong 250012, China
    Sci Rep 3:2333. 2013
    ..of metal ions on the phosphatase activity, we unexpectedly identified cadmium as a potent inhibitor of PPM1A and PPM1G. In contrast, low micromolar concentrations of cadmium did not inhibit PP1 or tyrosine phosphatases...
  34. pmc Examining post-translational modification-mediated protein-protein interactions using a chemical proteomics approach
    Xiang Li
    Laboratory of Chemistry and Cell Biology, New York, New York 10065, USA
    Protein Sci 22:287-95. 2013
    ..Together, our findings indicate the CLASPI approach can be broadly applied to profile protein-protein interactions mediated by PTMs...
  35. doi Molecular mechanisms underlying the interaction of protein phosphatase-1c with ASPP proteins
    Tamara D Skene-Arnold
    Department of Biochemistry, 3 37 Medical Sciences Building, School of Translational Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada, T6G 2H7
    Biochem J 449:649-59. 2013
    ..Overall, we provide the first direct evidence for PP-1c binding via its C-terminus to an SH3 protein domain...
  36. pmc Identification and characterization of protein phosphatase 2C activation by ceramide
    David M Perry
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Lipid Res 53:1513-21. 2012
    ..Proteomic analysis of this fraction revealed the identity of the phosphatase to be PP2Cγ/PPM1G. This was consistent with our findings that PP2Cγ 1-eluted in a high salt fraction due to its strongly acidic ..
  37. pmc Effects of a fluorescent Myosin light chain phosphatase inhibitor on prostate cancer cells
    Scott Grindrod
    Drug Discovery Program, Georgetown University Medical Center Washington, DC, USA
    Front Oncol 1:27. 2011
    ..We developed a fluorescent small molecule inhibitor of MLCP using structure based design in recombinant protein phosphatase 1C. Several best fit compounds were synthesized and evaluated by their inhibition of MLCP/(32)P-MLC ..
  38. pmc Lemur tyrosine kinase-2 signalling regulates kinesin-1 light chain-2 phosphorylation and binding of Smad2 cargo
    C Manser
    Department of Neuroscience P037, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, King s College London, London, UK
    Oncogene 31:2773-82. 2012
    ..We show that small interfering RNA loss of LMTK2 not only reduces binding of Smad2 to KLC2, but also inhibits TGFβ-induced Smad2 signalling. Thus, LMTK2 may regulate the activity of kinesin-1 motor function and Smad2 signalling...
  39. doi Cdk5/p35 phosphorylates lemur tyrosine kinase-2 to regulate protein phosphatase-1C phosphorylation and activity
    Catherine Manser
    Departments of Neuroscience and Clinical Neurosciences, Institute of Psychiatry, King s College London, London, UK
    J Neurochem 121:343-8. 2012
    ..We thus describe a new signalling pathway within the nervous system that links cdk5/p35 with PP1C and which has implications for a number of neuronal functions and neuronal dysfunction...
  40. pmc Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response
    Petra Beli
    The NNF Center for Protein Research, University of Copenhagen, Blegdamsvej 3b, DK 2200 Copenhagen, Denmark
    Mol Cell 46:212-25. 2012
    ..We show that the splicing-regulator phosphatase PPM1G is recruited to sites of DNA damage, while the splicing-associated protein THRAP3 is excluded from these regions...
  41. pmc Evidence for genes on chromosome 2 contributing to alcohol dependence with conduct disorder and suicide attempts
    Danielle M Dick
    Department of Psychiatry, Virginia Institute for Psychiatric and Behavioral Genetics, Virginia Commonwealth University, Richmond, VA 23298 0126, USA
    Am J Med Genet B Neuropsychiatr Genet 153:1179-88. 2010
    ..Further, none of the genes were located directly under the primary COGA linkage peak, which has implications for association tests following-up linkage peaks...
  42. ncbi Structure and regulation of the human Nek2 centrosomal kinase
    Peter Rellos
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford OX3 7LD, United Kingdom
    J Biol Chem 282:6833-42. 2007
    ..a role for dimerization-dependent allosteric regulation that combines with autophosphorylation and protein phosphatase 1c phosphatase activity to generate the precise spatial and temporal control required for Nek2 function in ..
  43. ncbi Regulation of protein phosphatase-1G from rabbit skeletal muscle. 1. Phosphorylation by cAMP-dependent protein kinase at site 2 releases catalytic subunit from the glycogen-bound holoenzyme
    M J Hubbard
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 186:701-9. 1989
    ....
  44. ncbi Genomic organization and functional analysis of the murine protein phosphatase 1c gamma (Ppp1cc) gene
    K Okano
    Department of Zoology, University of Toronto, Ontario, Canada
    Genomics 45:211-5. 1997
    ..Both isoforms retain phosphatase function since they are able to complement the cold-sensitive PP1 defect caused by the dis2-11 mutation in the fission yeast Schizosaccharomyces pombe...
  45. ncbi The NDUFS4 nuclear gene of complex I of mitochondria and the cAMP cascade
    Sergio Papa
    Department of Medical Biochemistry and Biology, University of Bari, Piazza Giulio Cesare n 11, 70124 Bari, Italy
    Biochim Biophys Acta 1555:147-53. 2002
    ..In the mitochondrial inner membrane a PP2Cgamma-type phosphatase has also been immunodetected, which dephosphorylates the 18 kDa subunit, phosphorylated by PKA...
  46. pmc Different binding motifs in metabotropic glutamate receptor type 7b for filamin A, protein phosphatase 1C, protein interacting with protein kinase C (PICK) 1 and syntenin allow the formation of multimeric protein complexes
    Ralf Enz
    Emil Fischer Zentrum, Institut fur Biochemie, Friedrich Alexander Universitat Erlangen Nurnberg, Fahrstrasse 17, D 91054 Erlangen, Germany
    Biochem J 372:183-91. 2003
    ..Our results represent a molecular description of the binding mechanisms of four mGluR7-associated proteins, and indicate the formation of ternary protein complexes composed of mGluR7b, PP1C, PICK1 and syntenin...
  47. ncbi Group I metabotropic glutamate receptors bind to protein phosphatase 1C. Mapping and modeling of interacting sequences
    Cristina Croci
    Emil Fischer Zentrum, Institut fur Biochemie, Friedrich Alexander Universitat Erlangen Nurnberg, Fahrstr 17, Erlangen 91054, Germany
    J Biol Chem 278:50682-90. 2003
    ..Here, we identified both catalytic gamma-isoforms of protein phosphatase 1C (PP1gamma1 and PP1gamma2) as binding partners of the group I metabotropic glutamate receptors type 1a, 5a, ..
  48. ncbi Functional characterization of the yeast Ppz1 phosphatase inhibitory subunit Hal3: a mutagenesis study
    Ivan Munoz
    Department de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Cerdanyola 08193, Barcelona, Spain
    J Biol Chem 279:42619-27. 2004
    ..It is unknown how Hal3 binds to Ppz1 and inhibits its activity. Although it contains a putative protein phosphatase 1c binding-like sequence (263KLHVLF268), mutagenesis analysis suggests that this motif is not required for ..
  49. ncbi Analysis on heat stress-induced hyperphosphorylation of stathmin at serine 37 in Jurkat cells by means of two-dimensional gel electrophoresis and tandem mass spectrometry
    Kazuyuki Nakamura
    Department of Biochemistry and Biomolecular Recognition, Yamaguchi University School of Medicine, Minami Kogushi 1 1 1, Ube, Yamaguchi 755 8505, Japan
    J Chromatogr A 1106:181-9. 2006
    ..The phosphorylation may cause the functional loss of stathmin for dynamic microtubule assembly and leads Jurkat cells to cell cycle arrest and apoptosis...
  50. ncbi Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L
    Jiayi Zhang
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, P R China
    Acta Biochim Pol 53:525-30. 2006
    ..transcripts of two adhesion-related genes (actin and parvin) and two apoptosis-related genes (cyclin 2 and protein phosphatase 1G) were up-regulated in AD293 cells...
  51. ncbi Oxytocin and parturition: a role for increased myometrial calcium and calcium sensitization?
    Patrice Arthur
    Perinatal Research Center, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Front Biosci 12:619-33. 2007
    ..and the 17-kDa PKC-potentiated inhibitor of protein phosphatase 1c (CPI-17)...
  52. doi New candidate targets of protein phosphatase-1c-gamma-2 in mouse testis revealed by a differential phosphoproteome analysis
    H Henderson
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Int J Androl 34:339-51. 2011
    ..Another potential target, tubulin, was found to be misregulated during Ppp1cc(-/-) spermatogenesis, disrupting manchette development. This work represents the first survey of the testicular phosphoproteome under pathological conditions...
  53. ncbi A testis specific isoform of endophilin B1, endophilin B1t, interacts specifically with protein phosphatase-1c gamma2 in mouse testis and is abnormally expressed in PP1c gamma null mice
    Christopher Hrabchak
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Biochemistry 46:4635-44. 2007
    ..A potential role for endophilin B1t in mammalian spermatogenesis is discussed within the context of the PP1c gamma knockout testis phenotype...
  54. ncbi Prostaglandin E2 induces vascular relaxation by E-prostanoid 4 receptor-mediated activation of endothelial nitric oxide synthase
    Ana Marija Hristovska
    Department of Physiology and Pharmacology, University of Southern Denmark, Odense, Denmark
    Hypertension 50:525-30. 2007
    ..Inhibitors of the cAMP-protein kinase A pathway attenuated, whereas the inhibitor of protein phosphatase 1C, calyculin (10(-8) mol/L), abolished the PGE(2)-mediated relaxation...
  55. doi FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16
    Kyu Heo
    Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089, USA
    Mol Cell 30:86-97. 2008
    ..Thus, these data establish FACT as the major regulator involved in H2AX exchange process that is modulated by H2AX phosphorylation and Spt16 ADP-ribosylation...
  56. pmc Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines
    Scoty M Hearst
    Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216, USA
    J Cell Sci 122:1872-81. 2009
    ..mRNA levels of the nuclear phosphatase PPM1G are significantly reduced in primary cells and expression of PPM1G in primary cells induces CBs...
  57. doi Protein phosphatase 2Cgamma regulates the level of p21Cip1/WAF1 by Akt signaling
    Eun Jung Suh
    Department of Biology, Kyung Hee University, 1 Hoegi dong Dongdaemoon ku, Seoul 130 701, Republic of Korea
    Biochem Biophys Res Commun 386:467-70. 2009
    b>PP2Cgamma is a splicing factor that dephosphorylates specific substrates required for the formation of the spliceosome...
  58. pmc Basis for the isoform-specific interaction of myosin phosphatase subunits protein phosphatase 1c beta and myosin phosphatase targeting subunit 1
    Elizabeth Scotto-Lavino
    Department of Pharmacology, Stony Brook University, Stony Brook, New York 11794 5140, USA
    J Biol Chem 285:6419-24. 2010
    ..Dephosphorylation is mediated by myosin phosphatase (MP), a complex that consists of a catalytic subunit (protein phosphatase 1c, PP1c), a large subunit (myosin phosphatase targeting subunit, MYPT), and a small subunit of unknown ..
  59. ncbi Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3
    P Dent
    Department of Biochemistry, Medical Sciences Institute, University of Dundee, Scotland
    FEBS Lett 248:67-72. 1989
    ....
  60. pmc Identification of a protective role for protein phosphatase 1cgamma1 against oxidative stress-induced vascular smooth muscle cell apoptosis
    Igor Tchivilev
    Department of Medicine, Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, North Carolina 27599 7126, USA
    J Biol Chem 283:22193-205. 2008
    ..Together, these data indicate that PP1cgamma1 overexpression promotes VSMC survival by interfering with JNK1 and p53 phosphorylation cascades involved in apoptosis...

Research Grants2

  1. Danielle M Dick; Fiscal Year: 2016
    ..abstract_text> ..
  2. Sodium Channel Blockers as Inhibitors of Prostate Cancer
    Milton Brown; Fiscal Year: 2008
    ..abstract_text> ..