POLN

Summary

Gene Symbol: POLN
Description: DNA polymerase nu
Alias: POL4P, DNA polymerase nu, DNA polymerase N, DNA polymerase POL4P, polymerase (DNA directed) nu, polymerase (DNA) nu
Species: human

Top Publications

  1. ncbi POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308
    Federica Marini
    University of Pittsburgh Cancer Institute, Hillman Cancer Center, Research Pavilion, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 278:32014-9. 2003
  2. pmc Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest
    Xiaohui Bi
    Department of Genetics and Genomics, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118, USA
    Mol Cell Biol 26:3527-40. 2006
  3. pmc A unique error signature for human DNA polymerase nu
    Mercedes E Arana
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 6:213-23. 2007
  4. pmc ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage
    Thomas Gohler
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, England, UK
    J Cell Biol 192:219-27. 2011
  5. doi Deciphering Carbamoylpolyoxamic Acid Biosynthesis Reveals Unusual Acetylation Cycle Associated with Tandem Reduction and Sequential Hydroxylation
    Jianzhao Qi
    Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China
    Cell Chem Biol 23:935-44. 2016
  6. pmc Structural and Kinetic Analysis of Miscoding Opposite the DNA Adduct 1,N6-Ethenodeoxyadenosine by Human Translesion DNA Polymerase η
    Amritraj Patra
    From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146
    J Biol Chem 291:14134-45. 2016
  7. pmc The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase η Mediates Its Interaction with the C-terminal Domain of Rev1
    Elizabeth M Boehm
    From the Departments of Biochemistry and
    J Biol Chem 291:8735-44. 2016
  8. pmc Mechanism of Ribonucleotide Incorporation by Human DNA Polymerase η
    Yan Su
    From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146
    J Biol Chem 291:3747-56. 2016
  9. pmc Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN)
    Kei ichi Takata
    From the Department of Epigenetics and Molecular Carcinogenesis, University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas 77030
    J Biol Chem 290:24278-93. 2015
  10. pmc Human DNA Polymerase ν Catalyzes Correct and Incorrect DNA Synthesis with High Catalytic Efficiency
    A S Prakasha Gowda
    From the Department of Biochemistry and Molecular Biology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
    J Biol Chem 290:16292-303. 2015

Research Grants

  1. Repair and Processing of DNA Crosslinks
    Richard Wood; Fiscal Year: 2007
  2. Mechanisms of DNA Lesion Bypass in Eukaryotes
    Zhigang Wang; Fiscal Year: 2006

Scientific Experts

  • Agnès Tissier
  • Kei ichi Takata
  • Richard D Wood
  • Zhigang Wang
  • Richard Wood
  • Mercedes E Arana
  • Yan Su
  • George Lucian Moldovan
  • Cyrus Vaziri
  • Laura R Barkley
  • Thomas A Kunkel
  • Masaoki Kohzaki
  • Michio Yoshimura
  • Elizabeth M Boehm
  • Martin Egli
  • F Peter Guengerich
  • Amritraj Patra
  • Jianzhao Qi
  • A S Prakasha Gowda
  • Jung Mi Park
  • Qian Song
  • Christopher B Whitehurst
  • Rémi Kazma
  • Xueying Sharon Liang
  • Leena Maddukuri
  • Thomas Gohler
  • Mark W Logue
  • Hwan Ki Park
  • Kinrin Yamanaka
  • Laura Zietlow
  • Eiichiro Sonoda
  • Mineaki Seki
  • Shunichi Takeda
  • Xiaohui Bi
  • Gudrun Schleiermacher
  • Paola Dugo
  • Wenqing Chen
  • Rong Gong
  • Xudong Qu
  • Federica Marini
  • Hongmin Ma
  • Maria Spies
  • Yuanzhen Liu
  • Kevin M Johnson
  • Zixin Deng
  • Qianqian Zhang
  • M Todd Washington
  • Kyle T Powers
  • Yuhui Sun
  • Christine M Kondratick
  • Jon C D Houtman
  • Dan Wan
  • Thomas E Spratt
  • Minoru Takata
  • Junya Tomida
  • Lisa M Swanhart
  • Neil A Hukriede
  • Shelley Reh
  • Seong Won Lee
  • Xuemin Sun
  • Jae Hong Seol
  • Young Joo Jeon
  • Seung Hyun Ka
  • Kyung Ryun Yu
  • Chin Ha Chung
  • Seung Wook Yang
  • Simone Benhamou
  • Sarah Eddy
  • Komaraiah Palle
  • Maroof K Zafar
  • Valerie Gaborieau
  • Andres Metspalu
  • Julia Shackelford
  • Rayjean J Hung
  • Endre Anderssen
  • Maiken B Elvestad
  • Zucai Suo
  • Xiaohong R Yang
  • Stephen Chanock
  • John R McLaughlin
  • Joseph S Pagano
  • Frank Skorpen
  • Naoko Kakusho
  • Amit Ketkar
  • John K Field
  • Alain Sarasin
  • Jianying Li
  • Dennis Maeder
  • Tonu Vooder
  • Ruth M Pfeiffer

Detail Information

Publications32

  1. ncbi POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308
    Federica Marini
    University of Pittsburgh Cancer Institute, Hillman Cancer Center, Research Pavilion, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 278:32014-9. 2003
    ..We report here the identification, cloning, and characterization of POLN and its gene product, a new mammalian DNA polymerase also related to Mus308...
  2. pmc Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest
    Xiaohui Bi
    Department of Genetics and Genomics, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118, USA
    Mol Cell Biol 26:3527-40. 2006
    ..Recruitment of Polkappa to ubiquitinated PCNA enables lesion bypass and eliminates stalled forks, thereby attenuating the S-phase checkpoint...
  3. pmc A unique error signature for human DNA polymerase nu
    Mercedes E Arana
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 6:213-23. 2007
    Human DNA polymerase nu (pol nu) is one of three A family polymerases conserved in vertebrates. Although its biological functions are unknown, pol nu has been implicated in DNA repair and in translesion DNA synthesis (TLS)...
  4. pmc ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage
    Thomas Gohler
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, England, UK
    J Cell Biol 192:219-27. 2011
    ..Taken together, our results provide evidence for a link between DNA damage-induced checkpoint activation and translesion synthesis in mammalian cells...
  5. doi Deciphering Carbamoylpolyoxamic Acid Biosynthesis Reveals Unusual Acetylation Cycle Associated with Tandem Reduction and Sequential Hydroxylation
    Jianzhao Qi
    Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China
    Cell Chem Biol 23:935-44. 2016
    ..We demonstrated that its biosynthesis is initiated by a versatile N-acetyltransferase, PolN, catalyzing L-glutamate (1) to N-acetyl glutamate (2)...
  6. pmc Structural and Kinetic Analysis of Miscoding Opposite the DNA Adduct 1,N6-Ethenodeoxyadenosine by Human Translesion DNA Polymerase η
    Amritraj Patra
    From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146
    J Biol Chem 291:14134-45. 2016
    ..Collectively, the results show a preference for purine pairing opposite 1,N(6)-ϵdA and for -1 frameshifts...
  7. pmc The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase η Mediates Its Interaction with the C-terminal Domain of Rev1
    Elizabeth M Boehm
    From the Departments of Biochemistry and
    J Biol Chem 291:8735-44. 2016
    ..These findings also suggest that PIP motifs are a more versatile protein interaction motif than previously believed. ..
  8. pmc Mechanism of Ribonucleotide Incorporation by Human DNA Polymerase η
    Yan Su
    From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146
    J Biol Chem 291:3747-56. 2016
    ..In addition, Tyr-92 was identified as a second line of defense to maintain the position of Phe-18. This is the first crystal structure of a DNA polymerase with an incoming rNTP opposite a DNA lesion. ..
  9. pmc Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN)
    Kei ichi Takata
    From the Department of Epigenetics and Molecular Carcinogenesis, University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas 77030
    J Biol Chem 290:24278-93. 2015
    DNA polymerase ν (POLN) is one of 16 DNA polymerases encoded in vertebrate genomes. It is important to determine its gene expression patterns, biological roles, and biochemical activities...
  10. pmc Human DNA Polymerase ν Catalyzes Correct and Incorrect DNA Synthesis with High Catalytic Efficiency
    A S Prakasha Gowda
    From the Department of Biochemistry and Molecular Biology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
    J Biol Chem 290:16292-303. 2015
    ..The presence of a mispair does not induce the polymerase to adopt a low catalytic conformation. pol ν catalyzes both correct and mispair formation with high catalytic efficiency. ..
  11. doi Modification of PCNA by ISG15 plays a crucial role in termination of error-prone translesion DNA synthesis
    Jung Mi Park
    School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151 742, South Korea
    Mol Cell 54:626-38. 2014
    ..These findings establish a crucial role of PCNA ISGylation in termination of error-prone TLS for preventing excessive mutagenesis. ..
  12. pmc Lung cancer and DNA repair genes: multilevel association analysis from the International Lung Cancer Consortium
    Rémi Kazma
    Universite Paris Sud, Villejuif, France
    Carcinogenesis 33:1059-64. 2012
    ..01): UBE2N, structural maintenance of chromosomes 1L2 and POLB. Two additional genes (RAD52 and POLN) are borderline significant...
  13. ncbi Uncoupling of 3'-phosphatase and 5'-kinase functions in budding yeast. Characterization of Saccharomyces cerevisiae DNA 3'-phosphatase (TPP1)
    J R Vance
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0602, USA
    J Biol Chem 276:15073-81. 2001
    ..plasmids by non-homologous end joining with considerable efficiency independently of the end-processing polymerase Pol4p. Homology searches and biochemical assays did not reveal a 5'-kinase that would account for this repair, however...
  14. ncbi Suppression of Saccharomyces cerevisiae rad27 null mutant phenotypes by the 5' nuclease domain of Escherichia coli DNA polymerase I
    Xuemin Sun
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Curr Genet 41:379-88. 2002
    ..the putative FEN-1 homologue in Escherichia coli is contained within the N-terminal region of the DNA polymerase I (PolN)...
  15. ncbi Stepwise occurrence of a complex unbalanced translocation in neuroblastoma leading to insertion of a telomere sequence and late chromosome 17q gain
    Gudrun Schleiermacher
    INSERM Unité 509, Laboratoire de Pathologie Moléculaire des Cancers, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Oncogene 24:3377-84. 2005
    ..the chromosome 4p telomere, and the (1;4) fusion disrupts the MACF1 (microtubule-actin crosslinking factor 1) and POLN genes, respectively...
  16. ncbi Determination of triacylglycerols in donkey milk by using high performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry
    Paola Dugo
    Dipartimento di Chimica Organica e Biologica, Facolta di Scienze, Universita di Messina, 98166 Messina, Italy
    J Sep Sci 28:1023-30. 2005
    ..Amongst the identified triacylglycerols, POLn, POO, PPO, CaPO, POL, and PPoO proved to be the main components of the TAG fraction of donkey milk.
  17. ncbi Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol
    Kei ichi Takata
    Department of Pharmacology, Hillman Cancer Center, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania 15213 1863, USA
    J Biol Chem 281:23445-55. 2006
    Human DNA polymerase N (POLN or pol nu) is the most recently discovered nuclear DNA polymerase in the human genome. It is an A-family DNA polymerase related to Escherichia coli pol I, human POLQ, and Drosophila Mus308...
  18. pmc Enhancement of human DNA polymerase η activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein
    Leena Maddukuri
    Department of Biochemistry and Molecular Biology, The University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 7199, USA
    J Biol Chem 287:42312-23. 2012
    ..In summary, WRN improves the efficiency and fidelity of hpol η to promote more effective replication of DNA...
  19. pmc Epstein-Barr virus BPLF1 deubiquitinates PCNA and attenuates polymerase η recruitment to DNA damage sites
    Christopher B Whitehurst
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 86:8097-106. 2012
    ..PCNA is the first cellular target identified for BPLF1 and its deubiquitinating activity...
  20. pmc c-Jun N-terminal kinase-mediated Rad18 phosphorylation facilitates Polη recruitment to stalled replication forks
    Laura R Barkley
    National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland
    Mol Biol Cell 23:1943-54. 2012
    ..Rad18 S409A also failed to fully complement the UV sensitivity of Rad18-depleted cells. Taken together, these results show that Rad18 phosphorylation by JNK represents a novel mechanism for promoting TLS and DNA damage tolerance...
  21. pmc Preparation of site-specific T=mCG cis-syn cyclobutane dimer-containing template and its error-free bypass by yeast and human polymerase η
    Qian Song
    Department of Chemistry, Washington University, St Louis, Missouri 63130, USA
    J Biol Chem 287:8021-8. 2012
    ..We also demonstrate that both yeast and human pol η synthesize past the 3'-(m)C CPD in a >99% error-free manner, consistent with the highly water-exposed nature of the active site...
  22. pmc A comprehensive genetic association study of Alzheimer disease in African Americans
    Mark W Logue
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Arch Neurol 68:1569-79. 2011
    ..To evaluate the association of genetic variation with late-onset Alzheimer disease (AD) in African Americans, including genes implicated in recent genome-wide association studies of whites...
  23. pmc Kinetic analysis of the unique error signature of human DNA polymerase ν
    Mercedes E Arana
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 50:10126-35. 2011
    ....
  24. pmc Genetic variants in DNA repair genes and the risk of cutaneous malignant melanoma in melanoma-prone families with/without CDKN2A mutations
    Xueying Sharon Liang
    Division of Cancer Epidemiology and Genetics, NCI NIH, Bethesda, MD, USA
    Int J Cancer 130:2062-6. 2012
    ..p-Values for SNPs in the same gene were combined to yield gene specific p-values. Two genes, POLN and PRKDC, were significantly associated with melanoma after Bonferroni correction for multiple testing (p = 0...
  25. pmc Novel enzymatic function of DNA polymerase nu in translesion DNA synthesis past major groove DNA-peptide and DNA-DNA cross-links
    Kinrin Yamanaka
    Department of Physiology and Pharmacology, Center for Research on Occupational and Environmental Toxicology, Oregon Health and Science University, L606, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Chem Res Toxicol 23:689-95. 2010
    b>DNA polymerase nu (POLN or pol nu) is a newly discovered A family polymerase that generates a high error rate when incorporating nucleotides opposite dG; its translesion DNA synthesis (TLS) capability has only been demonstrated for high ..
  26. pmc Evidence for the involvement of human DNA polymerase N in the repair of DNA interstrand cross-links
    Laura Zietlow
    The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Biochemistry 48:11817-24. 2009
    Human DNA polymerase N (PolN) is an A-family nuclear DNA polymerase whose function is unknown. This study examines the possible role of PolN in DNA repair in human cells treated with PolN-targeted siRNA...
  27. pmc Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage
    Michio Yoshimura
    Department of Radiation Genetics, CREST, Japan Science and Technology Laboratory, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Mol Cell 24:115-25. 2006
    ..We disrupted POLQ, and its homologs HEL308 and POLN in chicken DT40 cells, and also created polq/hel308 and polq/poln double mutants...
  28. pmc DNA polymerase POLN participates in cross-link repair and homologous recombination
    George Lucian Moldovan
    Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02215, USA
    Mol Cell Biol 30:1088-96. 2010
    ..We identified here the recently discovered polymerase POLN to be involved in repair of DNA cross-links...
  29. pmc Convergence of Rad6/Rad18 and Fanconi anemia tumor suppressor pathways upon DNA damage
    Hwan Ki Park
    The Hormel Institute, University of Minnesota, Austin, Minnesota, United States of America
    PLoS ONE 5:e13313. 2010
    ....
  30. pmc DNA polymerases nu and theta are required for efficient immunoglobulin V gene diversification in chicken
    Masaoki Kohzaki
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 189:1117-27. 2010
    ..Disruption of both polymerases causes a significant decrease in Ig gene conversion events, although POLN(-/-)/POLQ(-/-) cells exhibit no prominent defect in HR-mediated DNA repair, as indicated by no increase in ..
  31. doi Crosstalk between replicative and translesional DNA polymerases: PDIP38 interacts directly with Poleta
    Agnès Tissier
    CNRS, UPR, Conventionné par l Université d Aix Marseille, France
    DNA Repair (Amst) 9:922-8. 2010
    ..We thus suggest that PDIP38 serves as a mediator protein helping TLS Pols to transiently replace replicative polymerases at damaged sites...
  32. pmc Evolutionary conservation of residues in vertebrate DNA polymerase N conferring low fidelity and bypass activity
    Kei ichi Takata
    Department of Carcinogenesis, The University of Texas Graduate School of Biomedical Sciences at Houston, USA
    Nucleic Acids Res 38:3233-44. 2010
    b>POLN is a nuclear A-family DNA polymerase encoded in vertebrate genomes...

Research Grants2

  1. Repair and Processing of DNA Crosslinks
    Richard Wood; Fiscal Year: 2007
    ..Purified POLQ and POLN, a new enzyme discovered in our laboratory, will be tested for the ability to bypass DNA damage, including an ..
  2. Mechanisms of DNA Lesion Bypass in Eukaryotes
    Zhigang Wang; Fiscal Year: 2006
    ..We hypothesize that (a) Polzeta, Poln, Polkappa, and Rev1 are involved in DNA synthesis opposite the lesion in vivo; and (b) efficient bypass of some ..