PLA2G5

Summary

Gene Symbol: PLA2G5
Description: phospholipase A2 group V
Alias: FRFB, GV-PLA2, PLA2-10, hVPLA(2), calcium-dependent phospholipase A2, Ca2+-dependent phospholipase A2, phosphatidylcholine 2-acylhydrolase 5
Species: human

Top Publications

  1. ncbi Tagging SNP haplotype analysis of the secretory PLA2-V gene, PLA2G5, shows strong association with LDL and oxLDL levels, suggesting functional distinction from sPLA2-IIA: results from the UDACS study
    Peter T E Wootton
    Division of Cardiovascular Genetics, Department of Medicine, Royal Free and University College Medical School, 5 University Street, London WC1E 6JF, UK
    Hum Mol Genet 16:1437-44. 2007
  2. ncbi Transcellular secretion of group V phospholipase A2 from epithelium induces beta 2-integrin-mediated adhesion and synthesis of leukotriene C4 in eosinophils
    Nilda M Munoz
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, Chicago, IL 60637, USA
    J Immunol 177:574-82. 2006
  3. ncbi Systematic evaluation of transcellular activities of secretory phospholipases A2. High activity of group V phospholipases A2 to induce eicosanoid biosynthesis in neighboring inflammatory cells
    Gihani T Wijewickrama
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 281:10935-44. 2006
  4. ncbi Group V secretory phospholipase A2-modified low density lipoprotein promotes foam cell formation by a SR-A- and CD36-independent process that involves cellular proteoglycans
    Boris B Boyanovsky
    Department of Internal Medicine and Veterans Affairs Medical Center, Graduate Center for Nutritional Sciences, University of Kentucky Medical Center, Lexington, Kentucky 40536 0200, USA
    J Biol Chem 280:32746-52. 2005
  5. ncbi Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human
    J A Tischfield
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202 5251, USA
    Genomics 32:328-33. 1996
  6. pmc Group V phospholipase A2 mediates barrier disruption of human pulmonary endothelial cells caused by LPS in vitro
    Steven M Dudek
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, IL 60637, USA
    Am J Respir Cell Mol Biol 44:361-8. 2011
  7. ncbi Human group V phospholipase A2 induces group IVA phospholipase A2-independent cysteinyl leukotriene synthesis in human eosinophils
    Nilda M Munoz
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 278:38813-20. 2003
  8. ncbi Group V phospholipase A2 induces leukotriene biosynthesis in human neutrophils through the activation of group IVA phospholipase A2
    Young Jun Kim
    Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    J Biol Chem 277:36479-88. 2002
  9. pmc Group V secretory phospholipase A2 is involved in macrophage activation and is sufficient for macrophage effector functions in allergic pulmonary inflammation
    Shin Ohta
    Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 190:5927-38. 2013
  10. ncbi Cell-specific expression of group X and group V secretory phospholipases A(2) in human lung airway epithelial cells
    M C Seeds
    Departments of Internal Medicine Section on Pulmonary and Critical Care, Pathology, and Anesthesiology, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    Am J Respir Cell Mol Biol 23:37-44. 2000

Research Grants

  1. Barbara Balestrieri; Fiscal Year: 2016
  2. Marcia E Newcomer; Fiscal Year: 2014
  3. PHOSPHOLIPID TURNOVER IN EXOCRINE PANCREAS
    Ronald Rubin; Fiscal Year: 1991
  4. TRANSGENIC MOUSE MODELS OF PANCREATIC EXOCRINE FUNCTION
    ROBERT DE LISLE; Fiscal Year: 2003
  5. PATHOGENEISIS OF CYSTIC FIBROSIS IN THE GI SYSTEM
    ROBERT DE LISLE; Fiscal Year: 2003
  6. Structure Function Studies Of Membrane Binding Proteins
    Wonhwa Cho; Fiscal Year: 2004
  7. Studies of Diacylglycerol-Binding Proteins
    Wonhwa Cho; Fiscal Year: 2009

Scientific Experts

  • W Pruzanski
  • Galina D Mironova
  • Mario Menschikowski
  • Yi Wei
  • Steven M Dudek
  • Daniela N Amora
  • M C Seeds
  • Frances Bosetti
  • S A Farber
  • W Cho
  • Yukio Ishikawa
  • ROBERT DE LISLE
  • B Burlando
  • Lilian Leistad
  • E Mavoungou
  • L Levine
  • Hae Sook Shin
  • Barbara Balestrieri
  • Nilda M Munoz
  • Sonia Chalbot
  • Marcia E Newcomer
  • Ronald Rubin
  • Hiroyasu Sato
  • Alan R Leff
  • Makoto Murakami
  • Angelo Y Meliton
  • Khalid Iqbal
  • Kaj Blennow
  • Inge Grundke-Iqbal
  • Henrik Zetterberg
  • Tormod Fladby
  • Eva Hurt-Camejo
  • Young Jun Kim
  • João Luiz Silva-Filho
  • Munehiro Yamaguchi
  • Qi Huang
  • Ng Jun Bin
  • Koji Suzuki
  • Ichiro Kudo
  • Meilang Xue
  • Ewa Pniewska
  • Chun Ching Chiu
  • I Tamayo
  • Shin Ohta
  • Hae Jin Rhee
  • Massimiliano Mancini
  • Katariina Lähdesmäki
  • Daisuke Fujioka
  • Ho Sun Song
  • Wei Xing
  • Joshua A Boyce
  • Tetsuyuki Kobayashi
  • Kei Yamamoto
  • Kazutaka Ikeda
  • Yuki Isogai
  • Shuntaro Hara
  • Yoshitaka Taketomi
  • Panagiotis I Sergouniotis
  • Ryo Taguchi
  • Xiangdong Zhu
  • Giorgio Giannattasio
  • Indranil Chattopadhyay
  • H Ott
  • Michael H Gelb
  • C M Mounier
  • Peter T E Wootton
  • Seiko Masuda
  • Jonathan P Arm
  • Gihani T Wijewickrama
  • Toshiharu Ishii
  • Bruno L Diaz
  • Birgitta Rosengren
  • Frederick C de Beer
  • Michiko Mitsuishi
  • Nancy R Webb
  • Joseph V Bonventre
  • Fang Li
  • Badreddine Kriem
  • Ursula A Kessen
  • Boris B Boyanovsky
  • Evan Boetticher
  • E A Dennis
  • Edward A Dennis
  • Masamichi Ito
  • Leandro Souza Silva
  • Tanya M Laidlaw
  • Ana Acacia Sá Pinheiro
  • Jennifer Zacharia
  • Felipe Moraes-Santos
  • Gabriela Modenesi Sirtoli

Detail Information

Publications74

  1. ncbi Tagging SNP haplotype analysis of the secretory PLA2-V gene, PLA2G5, shows strong association with LDL and oxLDL levels, suggesting functional distinction from sPLA2-IIA: results from the UDACS study
    Peter T E Wootton
    Division of Cardiovascular Genetics, Department of Medicine, Royal Free and University College Medical School, 5 University Street, London WC1E 6JF, UK
    Hum Mol Genet 16:1437-44. 2007
    ..human studies suggest that both secretory PLA2 (sPLA2)-V and sPLA2-IIA (encoded, respectively, by the neighbouring PLA2G5 and PLA2G2A genes) contribute to atherogenesis...
  2. ncbi Transcellular secretion of group V phospholipase A2 from epithelium induces beta 2-integrin-mediated adhesion and synthesis of leukotriene C4 in eosinophils
    Nilda M Munoz
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, Chicago, IL 60637, USA
    J Immunol 177:574-82. 2006
    ..We also demonstrate that gVPLA(2), endogenously secreted from activated epithelial cells, promotes secretion of LTC(4) in cocultured eosinophils...
  3. ncbi Systematic evaluation of transcellular activities of secretory phospholipases A2. High activity of group V phospholipases A2 to induce eicosanoid biosynthesis in neighboring inflammatory cells
    Gihani T Wijewickrama
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 281:10935-44. 2006
    ....
  4. ncbi Group V secretory phospholipase A2-modified low density lipoprotein promotes foam cell formation by a SR-A- and CD36-independent process that involves cellular proteoglycans
    Boris B Boyanovsky
    Department of Internal Medicine and Veterans Affairs Medical Center, Graduate Center for Nutritional Sciences, University of Kentucky Medical Center, Lexington, Kentucky 40536 0200, USA
    J Biol Chem 280:32746-52. 2005
    ..Our data point to a physiological modification of LDL that has the potential to promote macrophage foam cell formation independent of scavenger receptors...
  5. ncbi Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human
    J A Tischfield
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202 5251, USA
    Genomics 32:328-33. 1996
    ..Unlike its rodent counterpart, human PLA2G2C appears to be a nonfunctional pseudogene...
  6. pmc Group V phospholipase A2 mediates barrier disruption of human pulmonary endothelial cells caused by LPS in vitro
    Steven M Dudek
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, IL 60637, USA
    Am J Respir Cell Mol Biol 44:361-8. 2011
    ....
  7. ncbi Human group V phospholipase A2 induces group IVA phospholipase A2-independent cysteinyl leukotriene synthesis in human eosinophils
    Nilda M Munoz
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 278:38813-20. 2003
    ..As such, this study represents a unique example in which a secretory phospholipase induces the eicosanoid formation in inflammatory cells, completely independent of cPLA2 activation...
  8. ncbi Group V phospholipase A2 induces leukotriene biosynthesis in human neutrophils through the activation of group IVA phospholipase A2
    Young Jun Kim
    Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    J Biol Chem 277:36479-88. 2002
    ....
  9. pmc Group V secretory phospholipase A2 is involved in macrophage activation and is sufficient for macrophage effector functions in allergic pulmonary inflammation
    Shin Ohta
    Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 190:5927-38. 2013
    We reported that Pla2g5-null mice lacking group V secretory phospholipase A2 (gV-sPLA2) showed reduced eosinophilic pulmonary inflammation and Th2 cytokine generation when challenged with an extract from house dust mite Dermatophagoides ..
  10. ncbi Cell-specific expression of group X and group V secretory phospholipases A(2) in human lung airway epithelial cells
    M C Seeds
    Departments of Internal Medicine Section on Pulmonary and Critical Care, Pathology, and Anesthesiology, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    Am J Respir Cell Mol Biol 23:37-44. 2000
    ....
  11. pmc PLA2G5 regulates transglutaminase activity of human IL-4-activated M2 macrophages through PGE2 generation
    Munehiro Yamaguchi
    Department of Medicine, Harvard Medical School, Boston Massachusetts, USA and the Jeff and Penny Vinik Center for Allergic Disease Research, Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Boston, Massachusetts, USA
    J Leukoc Biol 100:131-41. 2016
    ....
  12. doi Human group V secretory phospholipase A2 is associated with lipid rafts and internalized in a flotillin‑dependent pathway
    Hae Jin Rhee
    Institute of Natural Medicine, Hallym University, Chunchon, Kangwon Do 200 702, Republic of Korea
    Int J Mol Med 32:1126-36. 2013
    ....
  13. pmc Endothelial protein C receptor-associated invasiveness of rheumatoid synovial fibroblasts is likely driven by group V secretory phospholipase A2
    Meilang Xue
    Arthritis Res Ther 16:R44. 2014
    ..The aim of this study was to investigate the expression and function of EPCR on RASFs...
  14. pmc The adipocyte-inducible secreted phospholipases PLA2G5 and PLA2G2E play distinct roles in obesity
    Hiroyasu Sato
    Lipid Metabolism Project, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan Department of Health Chemistry, Showa University, School of Pharmacy, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    Cell Metab 20:119-32. 2014
    ..genes whose expressions are associated with obesity, we found that two secreted phospholipase A2s (sPLA2s), PLA2G5 and PLA2G2E, were robustly induced in adipocytes of obese mice...
  15. ncbi The expression of groups IIE and V phospholipase A2 is associated with an increased expression of osteogenic molecules in human calcified aortic valves
    Koji Suzuki
    Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine
    J Atheroscler Thromb 21:1308-25. 2014
    ..We therefore sought to determine whether PLA2s are expressed in human aortic valves and, if so, whether the expression of PLA2s is related to the expression of osteogenic molecules in these tissues...
  16. ncbi Compound 48/80 is a potent inhibitor of phospholipase C and a dual modulator of phospholipase A2 from human platelet
    C Bronner
    Biochim Biophys Acta 920:301-5. 1987
    ....
  17. pmc Group V Secretory Phospholipase A2 Is Involved in Tubular Integrity and Sodium Handling in the Kidney
    João Luiz Silva-Filho
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
    PLoS ONE 11:e0147785. 2016
    ..We used mice lacking the gene encoding GV sPLA2 (Pla2g5-/-) and wild-type breeding pairs in the experiments...
  18. doi Epigenetic control of group V phospholipase A2 expression in human malignant cells
    Mario Menschikowski
    Institute of Clinical Chemistry and Laboratory Medicine, Carl Gustav Carus University Hospital, Technical University of Dresden, Fetscherstr 74, D 01307, Dresden, Germany
    Tumour Biol 37:8097-105. 2016
    ..In conclusion, our results suggest that epigenetic mechanisms such as DNA methylation and histone modification play an important role in downregulation of GV-PLA2 expression in cancer cells. ..
  19. ncbi Edema-inducing activity of phospholipase A2 purified from human synovial fluid and inhibition by aristolochic acid
    B S Vishwanath
    Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298 0614
    Inflammation 12:549-61. 1988
    ..These results clearly demonstrate that the neutral-active, Ca2+-dependent PLA2 isolated from human synovial fluid is proinflammatory and that catalytic activity is positively correlated with in vivo proinflammatory effects...
  20. doi Group V secretory phospholipase A2 impairs endothelial protein C receptor-dependent protein C activation and accelerates thrombosis in vivo
    I Tamayo
    Division of Cardiovascular Sciences, Laboratory of Thrombosis and Hemostasis, Center for Applied Medical Research, University of Navarra, Pamplona, Spain
    J Thromb Haemost 12:1921-7. 2014
    ..Studies in purified systems have demonstrated that the substitution of PC notably impairs EPCR function in a process called EPCR encryption...
  21. doi Hydrolysis of lipoproteins by sPLA2's enhances mitogenesis and eicosanoid release from vascular smooth muscle cells: Diverse activity of sPLA2's IIA, V and X
    Waldemar Pruzanski
    St Michael s Hopital, Toronto, Canada University of Toronto, Toronto, Canada Electronic address
    Prostaglandins Other Lipid Mediat 122:64-8. 2016
    ..Our study demonstrates a diverse interaction of pro-inflammatory sPLA2's with HDL and LDL affecting both mitogenesis and eicosanoid release from VSMC, therefore potentially enhancing their pro-atherogenic activity. ..
  22. doi Phospholipase A2 group v in benign familial fleck retina in a set of triplets
    Ng Jun Bin
    Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia and Department of Ophthalmology, University Malaya Medical Center, Kuala Lumpur, Malaysia
    Retina 35:1266-72. 2015
    To evaluate the association of phospholipase A2, Group V (PLA2G5), with benign familial fleck retina in a consanguineous family with triplets.
  23. pmc Phylogenetic and structural analysis of the phospholipase A2 gene family in vertebrates
    Qi Huang
    Department of Neurology, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, P R China
    Int J Mol Med 35:587-96. 2015
    ..were observed between PLA2G16 and PLA2G6, PLA2G7 and PLA2G4, PLA2G3 and PLA2G12, as well as among PLA2G10, PLA2G5 and PLA2G15; ii) PLA2G5 appeared to be the origin of the PLA2 family, and PLA2G7 was one of the most ..
  24. pmc Effects of human Parvovirus B19 and Bocavirus VP1 unique region on tight junction of human airway epithelial A549 cells
    Chun Ching Chiu
    Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan Department of Neurology and Department of Medical Intensive Care Unit, Chunghua Christian Hospital, Chunghua, Taiwan
    PLoS ONE 9:e107970. 2014
    ..Meanwhile, this discrepancy appears not to be associated with the secreted phospholipase A2 (sPLA2)-like enzymatic activity. ..
  25. pmc The step further to understand the role of cytosolic phospholipase A2 alpha and group X secretory phospholipase A2 in allergic inflammation: pilot study
    Ewa Pniewska
    Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, 7 9, Zeligowskiego Street, 90 752 Lodz, Poland
    Biomed Res Int 2014:670814. 2014
    ..Summarizing, Der p1 and Fel d1 involve phospholipase A2 enzymes in their action. sPLA2X seems to be one of important PLA2 isoform in allergic inflammation, especially caused by house dust mite allergens. ..
  26. doi Competitive inhibition of cytosolic Ca2+-dependent phospholipase A2 by acteoside in RBL-2H3 cells
    Ho Sun Song
    College of Pharmacy, Chung Ang University, Seoul, Korea
    Arch Pharm Res 35:905-10. 2012
    ..9 μM and the inhibitory pattern appeared to be a competitive inhibitor. The above data, suggests that acteoside acts as a competitive inhibitor of cPLA(2) in RBL-2H3 cells...
  27. doi Mapping genetic determinants of coronary microvascular remodeling in the spontaneously hypertensive rat
    Massimiliano Mancini
    Department of Radiology, Oncology and Pathology, Sapienza University of Rome, Rome, Italy
    Basic Res Cardiol 108:316. 2013
    ..lt;5%), but not with blood pressure indices, were prioritized and four candidate genes were identified (Rtel1, Pla2g5, Dnaja4 and Rcn2) according to their expression levels and biological functions...
  28. doi Isoforms of secretory group two phospholipase A (sPLA2) in mouse ocular surface epithelia and lacrimal glands
    Yi Wei
    Department of Ophthalmology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Invest Ophthalmol Vis Sci 53:2845-55. 2012
    ..To compare and contrast the distribution patterns of select secretory group two phospholipase A (sPLA2) isoforms in corneal epithelia (CN), conjunctival epithelia (CNJ), and lacrimal glands (LG) of BALB/c and C57BL/6 mice...
  29. doi Acidity and lipolysis by group V secreted phospholipase A(2) strongly increase the binding of apoB-100-containing lipoproteins to human aortic proteoglycans
    Katariina Lähdesmäki
    Wihuri Research Institute, Kalliolinnantie 4, FIN 00140 Helsinki, Finland
    Biochim Biophys Acta 1821:257-67. 2012
    ..This is likely to provoke extracellular accumulation of lipids derived from these atherogenic lipoprotein particles and to increase the progression of the atherosclerotic lesions...
  30. pmc Distinct expression pattern of the full set of secreted phospholipases A2 in human colorectal adenocarcinomas: sPLA2-III as a biomarker candidate
    C M Mounier
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS UNSA UMR6097, Sophia Antipolis, Valbonne, France
    Br J Cancer 98:587-95. 2008
    ..In normal colon, PLA2G2A and PLA2G12A were expressed at high levels, PLA2G2D, PLA2G5, PLA2G10 and PLA2G12B at moderate levels, and PLA2G1B, PLA2G2F and PLA2G3 at low levels...
  31. pmc Analyses of group III secreted phospholipase A2 transgenic mice reveal potential participation of this enzyme in plasma lipoprotein modification, macrophage foam cell formation, and atherosclerosis
    Hiroyasu Sato
    Biomembrane Signaling Project, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Tokyo 113 8613, Japan
    J Biol Chem 283:33483-97. 2008
    ..showed that phosphatidylcholine was efficiently converted to lysophosphatidylcholine by PLA2G3 as well as by PLA2G5 and PLA2G10, to a lesser extent by PLA2G2F, and only minimally by PLA2G2A and PLA2G2E...
  32. doi Altered eicosanoid metabolism in the cystic fibrosis mouse small intestine
    Robert C De Lisle
    Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, KS 66160, USA
    J Pediatr Gastroenterol Nutr 47:406-16. 2008
    ..Imbalances in essential fatty acid levels have been reported in cystic fibrosis (CF), which may relate to elevated proinflammatory eicosanoid generation. The aim of this work was to better define eicosanoid metabolism in the CF intestine...
  33. ncbi Secretory phospholipase A2 group V: lesion distribution, activation by arterial proteoglycans, and induction in aorta by a Western diet
    Birgitta Rosengren
    AstraZeneca, R and D, Molecular Pharmacology, Mölndal S 431 83, Sweden
    Arterioscler Thromb Vasc Biol 26:1579-85. 2006
    ..In addition, we also investigated the effect of a Western diet and lipopolysaccharide challenge on the aortic expression of these enzymes in mouse models...
  34. pmc Secretory group V phospholipase A2 regulates acute lung injury and neutrophilic inflammation caused by LPS in mice
    Nilda M Munoz
    Dept of Medicine, M6076, Univ of Chicago, 5841 S Maryland Ave, Chicago, IL 60637, USA
    Am J Physiol Lung Cell Mol Physiol 296:L879-87. 2009
    ..Experiments were conducted in gVPLA(2) knockout (pla2g5(-/-)) mice, which lack the gene, and gVPLA(2) wild-type littermate control (pla2g5(+/+)) mice...
  35. pmc Cerebrospinal fluid secretory Ca2+-dependent phospholipase A2 activity is increased in Alzheimer disease
    Sonia Chalbot
    New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314 6399, USA
    Clin Chem 55:2171-9. 2009
    ....
  36. doi Genome-wide analysis of chromosomal alterations in patients with esophageal squamous cell carcinoma exposed to tobacco and betel quid from high-risk area in India
    Indranil Chattopadhyay
    Institute of Pathology, New Delhi 110029, India
    Mutat Res 696:130-8. 2010
    ..The candidate genes located at amplified regions of chromosomes or low-level gain regions such as PLA2G5 (1p36-p34), COL11A1 (1p21), KCNK2 (1q41), S100A3 (1q21), ENAH (1q42...
  37. doi High-resolution transcriptional profiling of chemical-stimulated dendritic cells identifies immunogenic contact allergens, but not prohaptens
    H Ott
    Department of Dermatology and Allergology, RWTH Aachen University Hospital, Aachen, Germany hagen ott post rwth aachen de
    Skin Pharmacol Physiol 23:213-24. 2010
    ..revealed coregulation of 11 marker genes which were related to immunological reactions (IL-8, CD1e, CD200R1, PLA2G5, TNFRSF11A), oxidative or metabolic stress responses (AKR1C3, SLC7A11, GCLM) or other processes (DPYLS3, TFPI, ..
  38. pmc Cerebrospinal fluid secretory Ca2+-dependent phospholipase A2 activity: a biomarker of blood-cerebrospinal fluid barrier permeability
    Sonia Chalbot
    New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314 6399, USA
    Neurosci Lett 478:179-83. 2010
    ..Though the CSF sPLA2 activity and Q(Alb) had over 95% agreement, the former was found to be more sensitive than the latter in measuring low levels of BCB impairment...
  39. pmc Group V secretory phospholipase A2 reveals its role in house dust mite-induced allergic pulmonary inflammation by regulation of dendritic cell function
    Giorgio Giannattasio
    Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 185:4430-8. 2010
    ..In this study, we report that group V sPLA(2) (Pla2g5)-null mice exposed to an extract of house dust mite Dermatophagoides farinae had markedly reduced pulmonary ..
  40. pmc Blood-cerebrospinal fluid barrier permeability in Alzheimer's disease
    Sonia Chalbot
    New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York, NY 10314 6399, USA
    J Alzheimers Dis 25:505-15. 2011
    ..These results suggest that the BCB damage occurs independent of and probably precedes both Aβ and tau pathologies in a restricted subgroup of AD patients...
  41. pmc Biallelic mutations in PLA2G5, encoding group V phospholipase A2, cause benign fleck retina
    Panagiotis I Sergouniotis
    Institute of Ophthalmology, University College London, London, UK
    Am J Hum Genet 89:782-91. 2011
    ..133G>T (p.Gly45Cys), in PLA2G5, a gene encoding a secreted phospholipase (group V phospholipase A(2))...
  42. ncbi Essential role of Ca2+ -dependent phospholipase A2 in estradiol-induced lysosome activation
    Bruno Burlando
    Dipartimento di Scienze e Tecnologie Avanzate, Universita del Piemonte Orientale Amedeo Avogadro, 15100 Alessandria, Biologia e Genetica, Universita di Genova, 16132 Genoa, Italy
    Am J Physiol Cell Physiol 283:C1461-8. 2002
    ..This mechanism promotes lysosome fusion and catabolic activities and may mediate short-term estradiol effects...
  43. ncbi Oligomers of prostaglandin B1 inhibit in vitro phospholipase A2 activity
    R C Franson
    Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298
    Biochim Biophys Acta 1006:272-7. 1989
    ....
  44. ncbi Cloning and recombinant expression of a novel human low molecular weight Ca(2+)-dependent phospholipase A2
    J Chen
    Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202
    J Biol Chem 269:2365-8. 1994
    ....
  45. ncbi HIV and SIV envelope glycoproteins induce phospholipase A2 activation in human and macaque lymphocytes
    E Mavoungou
    Department of Immunology and Immunogenetics, International Center for Medical Research, Franceville, Gabon
    J Acquir Immune Defic Syndr Hum Retrovirol 16:1-9. 1997
    ..However, this effect is not sufficient to accomplish fusion. These data indicate that the specific step of fusion may be downstream from PLA2 activation...
  46. ncbi Cloning and expression of a group IV cytosolic Ca2+-dependent phospholipase A2 from rat pancreatic islets. Comparison of the expressed activity with that of an islet group VI cytosolic Ca2+-independent phospholipase A2
    Z Ma
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Biochim Biophys Acta 1391:384-400. 1998
    ..The cPLA2 is thus one of at least three classes of PLA2 enzymes with distinct properties expressed in beta-cells...
  47. ncbi Expression and characterization of human group V phospholipase A2
    Y Chen
    Department of Chemistry and Biochemistry, School of Medicine and Revelle College, University of California, San Diego, La Jolla, CA 92093 0601, USA
    Biochim Biophys Acta 1394:57-64. 1998
    ....
  48. ncbi Large-scale sequencing of the rabbit corneal endothelial cDNA library
    T Fujimaki
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
    Cornea 18:109-14. 1999
    ..This study sought to identify novel or active genes in corneal endothelial cells with description of the gene-expression profile...
  49. ncbi Characterization of Ca2+-dependent phospholipase A2 activity during zebrafish embryogenesis
    S A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Biol Chem 274:19338-46. 1999
    ..Biol. Chem. 269, 18239-18249). By using a quenched BODIPY-labeled phosphatidylcholine that fluoresces only upon cleavage by PLA2, lipase activity was visualized in the cells of living embryos where it localized to perinuclear membranes...
  50. ncbi Low molecular weight group IIA and group V phospholipase A(2) enzymes have different intracellular locations in mouse bone marrow-derived mast cells
    C O Bingham
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 274:31476-84. 1999
    ..The spatial segregation of group IIA PLA(2) and group V PLA(2) implies that these enzymes are not functionally redundant...
  51. ncbi Expression of cytosolic and secreted forms of phospholipase A(2) and cyclooxygenases in human placenta, fetal membranes, and chorionic cell lines
    B Johansen
    UNIGEN Center for Molecular Biology, Department of Botany, Norwegian University of Science and Technology, N 7489, Trondheim, Norway
    Prostaglandins Other Lipid Mediat 60:119-25. 2000
    ..These results suggest that both cytosolic and distinct secreted forms of PLA(2) could be involved in arachidonic acid (AA) release preceding prostaglandin production at the fetal/maternal interface...
  52. pmc The molecular basis of phosphatidylcholine preference of human group-V phospholipase A2
    K P Kim
    Department of Chemistry M C 111, University of Illinois at Chicago, 845 West Taylor Street, Chicago, IL 60607 7061, USA
    Biochem J 348:643-7. 2000
    ....
  53. ncbi Structure, function, and regulation of group V phospholipase A(2)
    W Cho
    Department of Chemistry M C 111, University of Illinois at Chicago, 60607 7061, USA
    Biochim Biophys Acta 1488:48-58. 2000
    ..Finally, heparan sulfate proteoglycans in neutrophils appear to play a role of internalizing and degrading the cell surface-bound gVPLA(2) to protect the cells from extensive lipolytic damage...
  54. pmc VEGF stimulation of endothelial cell PAF synthesis is mediated by group V 14 kDa secretory phospholipase A2
    P N Bernatchez
    Research Center, Montreal Heart Institute, Montreal QC, Canada
    Br J Pharmacol 134:197-205. 2001
    ..7. These results provide evidence that with VEGF activation of EC cells, the group V sPLA2 provides substrate for EC PAF formation...
  55. ncbi Groups IV, V, and X phospholipases A2s in human neutrophils: role in eicosanoid production and gram-negative bacterial phospholipid hydrolysis
    Norbert Degousee
    Division of Vascular Surgery, Max Bell Research Center, Toronto General Hospital, University Health Network, Toronto M5G 2C4, Canada
    J Biol Chem 277:5061-73. 2002
    ....
  56. ncbi Human neutrophil migration in vitro induced by secretory phospholipases A2: a role for cell surface glycosaminoglycans
    Alessandra Gambero
    Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, PO Box 6111, 13081 970, Campinas, SP, Brazil
    Biochem Pharmacol 63:65-72. 2002
    ..Our results suggest that neutrophil migration in response to sPLA2s is independent of PLA activity, and involves an interaction of sPLA2s with cell surface heparin/heparan binding sites triggering the release of LTB4 and PAF...
  57. ncbi Purification and characterization of a cytosolic, 42-kDa and Ca2+-dependent phospholipase A2 from bovine red blood cells: its involvement in Ca2+-dependent release of arachidonic acid from mammalian red blood cells
    Hae Sook Shin
    Department of Environmental and Health Chemistry, College of Pharmacy, Chung Ang University, Dongjak Ku, Seoul 156 756, South Korea
    J Biol Chem 277:21086-94. 2002
    ..Together, our results suggest that the 42-kDa rPLA(2) identified as a novel form of Ca(2+)-dependent PLA(2) may play an important role in hemostasis, thrombosis, and/or erythropoiesis through the Ca(2+)-dependent release of AA...
  58. ncbi Deletion of secretory group V phospholipase A2 attenuates cell migration and airway hyperresponsiveness in immunosensitized mice
    Nilda M Munoz
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA
    J Immunol 179:4800-7. 2007
    ..Blockade of bronchoconstriction by MCL-3G1 was replicated in allergic pla2g5-/- mice, which lack the gene encoding gVPLA2...
  59. ncbi Role of three isoforms of phospholipase A2 in capacitative calcium influx in human T-cells
    Aziz Hichami
    UPRES Lipides and Nutrition, Université de Bourgogne 21000 Dijon, France Central Jalma Research Institute for Leprosy, Agra, UP, India
    Eur J Biochem 269:5557-63. 2002
    ..These results suggest that stimulation of three isoforms of PLA2 by thapsigargin liberates free AA that, in turn, induces capacitative calcium influx in human T-cells...
  60. ncbi Localization of group V phospholipase A2 in caveolin-enriched granules in activated P388D1 macrophage-like cells
    María A Balboa
    Department of Chemistry and Biochemistry, School of Medicine, University of California at San Diego, La Jolla, California 92093 0601, USA
    J Biol Chem 278:48059-65. 2003
    ....
  61. ncbi Presence of secretory group IIa and V phospholipase A2 and cytosolic group IValpha phospholipase A2 in chondrocytes from patients with rheumatoid arthritis
    Lilian Leistad
    Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    Clin Chem Lab Med 42:602-10. 2004
    ..These results suggest that each phospholipase A2 enzyme has distinct roles in both healthy and diseased joint tissue, and that the diversity and amount of enzyme correlate with the grade of inflammation and disease severity...
  62. ncbi Distribution of type V secretory phospholipase A2 expression in human hepatocytes damaged by liver disease
    Masamichi Ito
    Department of Pathology, Toho University School of Medicine, Tokyo, Japan
    J Gastroenterol Hepatol 19:1140-9. 2004
    ..However, the distribution of sPLA2-V in human liver has not yet been investigated. In this study, the significance of sPLA2-V expression in human hepatocytes damaged by liver disease was investigated...
  63. ncbi Cytosolic phospholipase A2 mediates neuronal apoptosis induced by soluble oligomers of the amyloid-beta peptide
    Badreddine Kriem
    INSERM EMI 0014, Universite de Nancy I, Vandoeuvre, France
    FASEB J 19:85-7. 2005
    ..This may prove to be crucial in AD processes and could provide important targets for drug development...
  64. ncbi Prostaglandin E2 and microsomal prostaglandin E synthase-2 expression are decreased in the cyclooxygenase-2-deficient mouse brain despite compensatory induction of cyclooxygenase-1 and Ca2+-dependent phospholipase A2
    Francesca Bosetti
    Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Neurochem 91:1389-97. 2004
    ..Overall, the data suggest that compensatory mechanisms exist in COX-2(-/-) mice and that mPGES-2 is functionally coupled with COX-2...
  65. ncbi Angiotensin II-induced Akt activation is mediated by metabolites of arachidonic acid generated by CaMKII-stimulated Ca2(+)-dependent phospholipase A2
    Fang Li
    Department of Pharmacology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Am J Physiol Heart Circ Physiol 288:H2306-16. 2005
    ....
  66. pmc Tetrandrine and thapsigargin release arachidonic acid from cells in culture and stimulate prostacyclin production in rat liver cells, but may do so by different pathways
    Lawrence Levine
    Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    BMC Pharmacol 5:12. 2005
    ..The effects of tetrandrine and thapsigargin on arachidonic acid release from human colon carcinoma and rat liver cells and prostacyclin production by rat liver cells are compared in the current studies...
  67. ncbi Expression of type V secretory phospholipase A in myocardial remodelling after infarction
    Y Ishikawa
    Department of Pathology, School of Dentistry, Nihon University, Tokyo, Japan
    Histopathology 47:257-67. 2005
    ..The significance of sPLA2-V in myocardial infarction was investigated histopathologically...
  68. pmc Group V and X secretory phospholipase A2 prevents adenoviral infection in mammalian cells
    Michiko Mitsuishi
    Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    Biochem J 393:97-106. 2006
    ....
  69. ncbi PGE2 release is independent of upregulation of Group V phospholipase A2 during long-term stimulation of P388D1 cells with LPS
    Ursula A Kessen
    Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA
    J Lipid Res 46:2488-96. 2005
    ..LPS stimulation also results in increased expression and secretion of a secretory PLA2, specifically GV PLA2. To test whether GV PLA2 contributes to PGE2 production and whether GIVA PLA2 activation increases the expression ..
  70. ncbi Effects of Crotalus durissus collilineatus venom in the isolated rat kidney
    Daniela N Amora
    Department of Physiology and Pharmacology, Institute of Biomedicine and Clinical Research Unit, Federal University of Ceara, Fortaleza, Ceara, Brazil
    Toxicon 47:260-4. 2006
    ..We suggest that crotoxin and phospholipase A2 were involved in this process, since the renal effects observed would be due to the synergistic action of the components of the venom...
  71. pmc Fusion of neurotransmitter vesicles with target membrane is calcium independent in a cell-free system
    U O Karli
    Friedrich Miescher Institut, Basel, Switzerland
    Proc Natl Acad Sci U S A 87:5912-5. 1990
    ....
  72. ncbi Inflammation and atherosclerosis: Group IIa and Group V sPLA2 are not redundant
    Frederick C de Beer
    Arterioscler Thromb Vasc Biol 26:1421-2. 2006
  73. pmc Group V secretory phospholipase A2 amplifies the induction of cyclooxygenase 2 and delayed prostaglandin D2 generation in mouse bone marrow culture-derived mast cells in a strain-dependent manner
    Bruno L Diaz
    Department of Medicine Harvard Medical School and the Division of Rheumatology Immunology and Allergy, Brigham and Women s Hospital, Boston, MA 02115, USA
    Biochim Biophys Acta 1761:1489-97. 2006
    ..We used mice lacking the gene encoding group V sPLA(2) (Pla2g5-/-) to definitively test its role in eicosanoid generation by BMMC...

Research Grants8

  1. Barbara Balestrieri; Fiscal Year: 2016
    ..Now our preliminary data indicate that mice genetically modified to lack group V sPLA2 (Pla2g5-null mice) do not develop alternative activated macrophages (AAMacs) in the lung following Df exposure and that ..
  2. Marcia E Newcomer; Fiscal Year: 2014
    ..The proposed studies will provide both structural and mechanistic information for this key enzyme. ..
  3. PHOSPHOLIPID TURNOVER IN EXOCRINE PANCREAS
    Ronald Rubin; Fiscal Year: 1991
    ..These studies should render support for the association between inositol phospholipid breakdown mediated by PLC and PLA2, CA2+ metabolism, protein phosphorylation, and amylase secretion...
  4. TRANSGENIC MOUSE MODELS OF PANCREATIC EXOCRINE FUNCTION
    ROBERT DE LISLE; Fiscal Year: 2003
    ..That over expresses Muclin in the pancreatic acinar cell using the rat elastase promoter to direct cell specific expression. ..
  5. PATHOGENEISIS OF CYSTIC FIBROSIS IN THE GI SYSTEM
    ROBERT DE LISLE; Fiscal Year: 2003
    ..Pathology and Muclin expression will be compared in CF mice, and CF mice crossed with gastrin deficient mice (lack gastric acid secretion) to normalize intestinal Ph. ..
  6. Structure Function Studies Of Membrane Binding Proteins
    Wonhwa Cho; Fiscal Year: 2004
    ....
  7. Studies of Diacylglycerol-Binding Proteins
    Wonhwa Cho; Fiscal Year: 2009
    ....