PLA2G4A

Summary

Gene Symbol: PLA2G4A
Description: phospholipase A2 group IVA
Alias: PLA2G4, cPLA2, cPLA2-alpha, cytosolic phospholipase A2, calcium-dependent phospholipid-binding protein, lysophospholipase, phosphatidylcholine 2-acylhydrolase, phospholipase A2, group IVA (cytosolic, calcium-dependent)
Species: human

Top Publications

  1. ncbi Cleavage of human cytosolic phospholipase A2 by caspase-1 (ICE) and caspase-8 (FLICE)
    S Lüschen
    Institut fur Immunologie, Christian Albrechts Universitat Kiel, Germany
    Biochem Biophys Res Commun 253:92-8. 1998
  2. pmc The RNA-binding protein HuR stabilizes cytosolic phospholipase A2α mRNA under interleukin-1β treatment in non-small cell lung cancer A549 Cells
    Wan Lin Liao
    Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan
    J Biol Chem 286:35499-508. 2011
  3. doi Cigarette smoke extract regulates cytosolic phospholipase A2 expression via NADPH oxidase/MAPKs/AP-1 and p300 in human tracheal smooth muscle cells
    Shin Ei Cheng
    Department of Physiology and Pharmacology, Chang Gung University, Kwei San, Tao Yuan, Taiwan
    J Cell Biochem 112:589-99. 2011
  4. ncbi Attenuated translocation of group IVa phospholipase A2 and up-regulated annexin-1 synthesis by glucocorticoid blocks beta 2-integrin adhesion in neutrophils
    Angelo Y Meliton
    Department of Medicine, The University of Chicago, Chicago, IL 60637, USA
    J Leukoc Biol 83:344-51. 2008
  5. ncbi A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP
    J D Clark
    Genetics Institute, Inc, Cambridge, Massachusetts 02140
    Cell 65:1043-51. 1991
  6. ncbi Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2
    J D Sharp
    Lilly Research Laboratories, Indianapolis, Indiana 46285
    J Biol Chem 269:23250-4. 1994
  7. ncbi Mechanism of group IVA cytosolic phospholipase A(2) activation by phosphorylation
    Sudipto Das
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 278:41431-42. 2003
  8. ncbi Cytosolic phospholipase A2 translocates to forming phagosomes during phagocytosis of zymosan in macrophages
    Milena Girotti
    Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 279:19113-21. 2004
  9. ncbi Cytosolic phospholipase A2 Group IValpha but not secreted phospholipase A2 Group IIA, V, or X induces interleukin-8 and cyclooxygenase-2 gene and protein expression through peroxisome proliferator-activated receptors gamma 1 and 2 in human lung cells
    Rafal Pawliczak
    Critical Care Medicine Department, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:48550-61. 2004
  10. ncbi Activation of cPLA2 is required for leukotriene D4-induced proliferation in colon cancer cells
    Ladan Parhamifar
    Experimental Pathology, The Department of Laboratory Medicine and Surgery, Lund University, Malmo University Hospital, Malmo, Sweden
    Carcinogenesis 26:1988-98. 2005

Research Grants

  1. Role of a Novel Lysophospholipase in Tumorigenesis
    Ying Huang; Fiscal Year: 2012
  2. Dennis E Hallahan; Fiscal Year: 2014
  3. LIPID METABOLISM IN STIMULATED HUMAN PLATELETS
    M Broekman; Fiscal Year: 1991
  4. Characterization of a Novel Lysophospholipase
    Ying Huang; Fiscal Year: 2003
  5. Susan S Smyth; Fiscal Year: 2014
  6. Regulation of Lysolipid Signaling by Autotaxin-Integrin Interactions on Platelets
    ZACHARY FULKERSON; Fiscal Year: 2013
  7. Lysolipid Signaling in Cardiovascular Disease
    Susan S Smyth; Fiscal Year: 2013
  8. Arie Kaffman; Fiscal Year: 2014
  9. Gabor J Tigyi; Fiscal Year: 2016
  10. ERYTHROCYTE METABOLISM IN MUSCULAR DISEASE
    Akira Omachi; Fiscal Year: 1980

Detail Information

Publications260 found, 100 shown here

  1. ncbi Cleavage of human cytosolic phospholipase A2 by caspase-1 (ICE) and caspase-8 (FLICE)
    S Lüschen
    Institut fur Immunologie, Christian Albrechts Universitat Kiel, Germany
    Biochem Biophys Res Commun 253:92-8. 1998
    ..In a previous study, we have reported that human cPLA2 is proteolytically inactivated during apoptosis through cleavage by a caspase-3-like activity...
  2. pmc The RNA-binding protein HuR stabilizes cytosolic phospholipase A2α mRNA under interleukin-1β treatment in non-small cell lung cancer A549 Cells
    Wan Lin Liao
    Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan
    J Biol Chem 286:35499-508. 2011
    ..This inhibitory effect was also observed in binding with COX-2 mRNA. This result indicated that p38 MAPK-mediated Thr-118 phosphorylation may play a key role in regulating the interaction of HuR with its target mRNAs in inflammation...
  3. doi Cigarette smoke extract regulates cytosolic phospholipase A2 expression via NADPH oxidase/MAPKs/AP-1 and p300 in human tracheal smooth muscle cells
    Shin Ei Cheng
    Department of Physiology and Pharmacology, Chang Gung University, Kwei San, Tao Yuan, Taiwan
    J Cell Biochem 112:589-99. 2011
    ..These results demonstrated that CSE-induced cPLA(2) expression was mediated through NOX2-dependent p42/p44 MAPK and p38 MAPK/c-Fos and JNK1/2/c-Jun/p300 pathways in HTSMCs...
  4. ncbi Attenuated translocation of group IVa phospholipase A2 and up-regulated annexin-1 synthesis by glucocorticoid blocks beta 2-integrin adhesion in neutrophils
    Angelo Y Meliton
    Department of Medicine, The University of Chicago, Chicago, IL 60637, USA
    J Leukoc Biol 83:344-51. 2008
    ....
  5. ncbi A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP
    J D Clark
    Genetics Institute, Inc, Cambridge, Massachusetts 02140
    Cell 65:1043-51. 1991
    We report the cloning and expression of a cDNA encoding a high molecular weight (85.2 kd) cytosolic phospholipase A2 (cPLA2) that has no detectable sequence homology with the secreted forms of PLA2...
  6. ncbi Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2
    J D Sharp
    Lilly Research Laboratories, Indianapolis, Indiana 46285
    J Biol Chem 269:23250-4. 1994
    The Ca(2+)-sensitive cytosolic phospholipase A2 (cPLA2) displays both a phospholipase A2 and a lysophospholipase activity...
  7. ncbi Mechanism of group IVA cytosolic phospholipase A(2) activation by phosphorylation
    Sudipto Das
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 278:41431-42. 2003
    Group IVA cytosolic phospholipase A2 (cPLA2) has been shown to play a critical role in the agonist-induced release of arachidonic acid...
  8. ncbi Cytosolic phospholipase A2 translocates to forming phagosomes during phagocytosis of zymosan in macrophages
    Milena Girotti
    Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 279:19113-21. 2004
    ..The results demonstrate that cPLA(2)alpha targets the phagosome membrane, which may serve as a source of arachidonic acid for eicosanoid production...
  9. ncbi Cytosolic phospholipase A2 Group IValpha but not secreted phospholipase A2 Group IIA, V, or X induces interleukin-8 and cyclooxygenase-2 gene and protein expression through peroxisome proliferator-activated receptors gamma 1 and 2 in human lung cells
    Rafal Pawliczak
    Critical Care Medicine Department, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:48550-61. 2004
    ..We have shown previously that cytosolic phospholipase A2 (cPLA2) is able to activate gene expression through PPAR-gamma response elements (Pawliczak, R., Han, C...
  10. ncbi Activation of cPLA2 is required for leukotriene D4-induced proliferation in colon cancer cells
    Ladan Parhamifar
    Experimental Pathology, The Department of Laboratory Medicine and Surgery, Lund University, Malmo University Hospital, Malmo, Sweden
    Carcinogenesis 26:1988-98. 2005
    ..The present data reveal that cPLA(2)alpha, an important intracellular signal activated by inflammatory mediators, is an important regulator of colon tumor growth...
  11. doi Group IVA phospholipase A2 is necessary for the biogenesis of lipid droplets
    Albert Gubern
    Institut de Neurociències and Departament de Bioquímica i Biologia Molecular, Universitat Autonoma de Barcelona, E 08193 Barcelona, Spain
    J Biol Chem 283:27369-82. 2008
    ....
  12. pmc Role of phosphorylation and basic residues in the catalytic domain of cytosolic phospholipase A2alpha in regulating interfacial kinetics and binding and cellular function
    Dawn E Tucker
    Program in Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colorado 80206, USA
    J Biol Chem 284:9596-611. 2009
    ..The results demonstrate that Ser-505 phosphorylation and basic residues in the catalytic domain principally act to regulate cPLA(2)alpha hydrolytic activity...
  13. pmc Group IV phospholipase A(2)alpha controls the formation of inter-cisternal continuities involved in intra-Golgi transport
    Enrica San Pietro
    Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Chieti, Italy
    PLoS Biol 7:e1000194. 2009
    ..These findings identify cPLA(2)alpha as the first component of the machinery that is responsible for the formation of intercisternal tubular continuities and support a role for these continuities in transport through the Golgi complex...
  14. pmc Impacts of cytosolic phospholipase A2, 15-prostaglandin dehydrogenase, and cyclooxygenase-2 expressions on tumor progression in colorectal cancer
    Sung Chul Lim
    Department of Pathology, College of Medicine, Chosun University, Gwangju, Korea
    Yonsei Med J 51:692-9. 2010
    ..to cyclooxygenase-2 (COX-2) which is related to prostaglandin E2 synthesis, other enzymes such as cytosolic phospholipase A2 (cPLA2), microsomal prostaglandin E2 synthase-1 (mPGES-1), and 15-prostaglandin dehydrogenase (15-PGDH) ..
  15. doi HT-29 human colon cancer cell proliferation is regulated by cytosolic phospholipase A(2)α dependent PGE(2)via both PKA and PKB pathways
    Liz Kisslov
    Clinical Biochemistry Department, Soroka Medical University Center, Beer Sheva, Israel
    Biochim Biophys Acta 1821:1224-34. 2012
    ..PGE(2)via EP induces CREB phosphorylation by the PKA pathway and regulates β-catenin and cyclin D1 cellular localization by PKB/Akt pathway...
  16. ncbi cPLA2 is phosphorylated and activated by MAP kinase
    L L Lin
    Genetics Institute, Small Molecule Drug Discovery Group, Cambridge, Massachusetts 02140
    Cell 72:269-78. 1993
    ..that stimulate the release of arachidonic acid causes increased serine phosphorylation and activation of cytosolic phospholipase A2 (cPLA2). Here we report that cPLA2 is a substrate for mitogen-activated protein (MAP) kinase...
  17. ncbi Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2. Probing the role of histidine, aspartic acid, cysteine, and arginine
    R T Pickard
    Lilly Research Laboratories, Indianapolis, Indiana 46285, USA
    J Biol Chem 271:19225-31. 1996
    b>Cytosolic phospholipase A2 (cPLA2) hydrolyzes the sn-2-acyl ester bond of phospholipids and shows a preference for arachidonic acid-containing substrates...
  18. ncbi Expression of cytosolic and group X secretory phospholipase A(2) genes in human colorectal adenocarcinomas
    Anna Osterström
    Department of Biomedicine and Surgery, Division of Cell Biology, Faculty of Health Sciences, Linkoping, Sweden
    Cancer Lett 182:175-82. 2002
    ..We found an up-regulation of cPLA(2) mRNA but the induction in tumour tissues does not correlate with Ras-gene mutations. Moreover, our results cannot consistently reflect an overexpression of sPLA(2)-X gene in colorectal cancer tissues...
  19. ncbi The confluence-dependent interaction of cytosolic phospholipase A2-alpha with annexin A1 regulates endothelial cell prostaglandin E2 generation
    Shane P Herbert
    Faculty of Biological Sciences, Institute of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom
    J Biol Chem 282:34468-78. 2007
    ..Thus the confluence-dependent interaction of cPLA(2)alpha and annexin A1 at the Golgi acts as a novel molecular switch controlling cPLA(2)alpha activity and endothelial cell prostaglandin generation...
  20. doi Impact of group IVA cytosolic phospholipase A2 gene polymorphisms on phenotypic features of patients with familial adenomatous polyposis
    Junji Umeno
    Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka, 812 8582, Japan
    Int J Colorectal Dis 25:293-301. 2010
    ..The aim of this study was to elucidate the possible associations between cPLA ( 2 )alpha gene polymorphisms and phenotypic features of patients with familial adenomatous polyposis (FAP)...
  21. ncbi Nuclear localisation of cytosolic phospholipase A2-alpha in the EA.hy.926 human endothelial cell line is proliferation dependent and modulated by phosphorylation
    Seema Grewal
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK
    J Cell Sci 115:4533-43. 2002
    ....
  22. pmc Phosphatidylinositol 4,5-bisphosphate anchors cytosolic group IVA phospholipase A2 to perinuclear membranes and decreases its calcium requirement for translocation in live cells
    Javier Casas
    Institute of Molecular Biology and Genetics, Spanish Research Council, 47003 Valladolid, Spain
    Mol Biol Cell 17:155-62. 2006
    ..PtdIns(4,5)P2 anchors the enzyme to perinuclear membranes and allows for a proper interaction with its phospholipid substrate to release arachidonic acid...
  23. ncbi Cigarette smoke extract induces cytosolic phospholipase A2 expression via NADPH oxidase, MAPKs, AP-1, and NF-kappaB in human tracheal smooth muscle cells
    Shin Ei Cheng
    Department of Pharmacology, Chang Gung University, Kwei San, Tao Yuan, Taiwan
    Free Radic Biol Med 46:948-60. 2009
    Up-regulation of cytosolic phospholipase A2 (cPLA2) by cigarette smoke extract (CSE) may play a critical role in airway inflammatory diseases...
  24. pmc Ceramide 1-phosphate is required for the translocation of group IVA cytosolic phospholipase A2 and prostaglandin synthesis
    Nadia F Lamour
    Department of Biochemistry, Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298 0614, USA
    J Biol Chem 284:26897-907. 2009
    ..Therefore, this study provides a paradigm shift in how cPLA(2)alpha is activated during inflammation...
  25. pmc Golgi-associated cPLA2alpha regulates endothelial cell-cell junction integrity by controlling the trafficking of transmembrane junction proteins
    Elsa Regan-Klapisz
    Cellular Architecture and Dynamics, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Mol Biol Cell 20:4225-34. 2009
    ....
  26. doi Subcellular localization and role of lipin-1 in human macrophages
    Martín Valdearcos
    Instituto de Biologia y Genetica Molecular, Consejo Superior de Investigaciones Cientificas, 47003 Valladolid, Spain
    J Immunol 186:6004-13. 2011
    ..Collectively, these data suggest that lipin-1 associates with LDs and regulates the activation of cytosolic group IVA phospholipase A(2)α in human monocyte-derived macrophages...
  27. pmc cPLA(2)α gene activation by IL-1β is dependent on an upstream kinase pathway, enzymatic activation and downstream 15-lipoxygenase activity: a positive feedback loop
    Jewell N Walters
    Department of Neuroscience, University of Florida, Gainesville, FL 32610, USA
    Cell Signal 23:1944-51. 2011
    ..Overall, these data support an MKK3/MKK6→p38 MAPK→MSK-1→cPLA(2)α→15-LOX2-dependent, positive feedback loop where a protein's enzymatic activity is required to regulate its own gene induction by a pro-inflammatory stimulus...
  28. ncbi Identification of a cellular protein that functionally interacts with the C2 domain of cytosolic phospholipase A(2)alpha
    Y Nakatani
    Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Biol Chem 275:1161-8. 2000
    ..These results suggest that vimentin is an adaptor for cPLA(2)alpha to function properly during the eicosanoid-biosynthetic process...
  29. doi The manifold phospholipases A of Legionella pneumophila - identification, export, regulation, and their link to bacterial virulence
    Sangeeta Banerji
    Research Group Pathogenesis of Legionella Infections, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Int J Med Microbiol 298:169-81. 2008
    ..lung pathogen Legionella pneumophila expresses secreted and cell-associated phospholipase A (PLA) and lysophospholipase A (LPLA) activities belonging to at least three enzyme families...
  30. ncbi Purification of a new, calcium-independent, high molecular weight phospholipase A2/lysophospholipase (phospholipase B) from guinea pig intestinal brush-border membrane
    A Gassama-Diagne
    Institut National de la Santé et de la Recherche Médicale Unité 101, Biochimie des Lipides, Toulouse, France
    J Biol Chem 264:9470-5. 1989
    ..the two hydrolytic activities were detected at the same position as 97-kDa protein, the enzyme is thus considered as a phospholipase A2 with lysophospholipase activity (phospholipase B), which might be involved in phospholipid digestion.
  31. pmc Distinct phospholipase A2 enzymes regulate prostaglandin E2 and F2alpha production by bovine endometrial epithelial cells
    Patricia K Tithof
    The University of Tennessee College of Veterinary Medicine, TN, USA
    Reprod Biol Endocrinol 5:16. 2007
    ..the expression and activities of calcium-independent Group VI and Group IVC PLA2 (PLA2G6 and PLA2G4C) and calcium-dependent Group IVA PLA2 (PLA2G4A) enzymes in the regulation of bovine uterine endometrial epithelial cell PG production.
  32. pmc Phospholipase A2 regulation of bovine endometrial (BEND) cell prostaglandin production
    James D Godkin
    Department of Animal Science, The University of Tennessee, Knoxville, TN, USA
    Reprod Biol Endocrinol 6:44. 2008
    ..The objectives of this investigation were to evaluate the expression and activities of calcium-dependent group IVA (PLA2G4A) and calcium-independent group VI (PLA2G6) enzymes in the regulation of BEND cell PG production.
  33. ncbi Further characterization of a novel phospholipase B (phospholipase A2--lysophospholipase) from intestinal brush-border membranes
    S Pind
    Department of Biochemistry, University of Toronto, Ont, Canada
    Biochem Cell Biol 69:346-57. 1991
    ..and guinea pigs possess a high molecular weight, calcium-independent phospholipase B (phospholipase A2 - lysophospholipase activities) with the characteristics of a digestive ectoenzyme...
  34. pmc Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans
    S C Chen
    Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Westmead, NSW 2145, Australia
    Biochem J 347:431-9. 2000
    ..A highly active extracellular phospholipase, demonstrating phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities, was purified to homogeneity from C...
  35. ncbi Lysophospholipase from the human blood fluke, Schistosoma japonicum
    Jinjiang Fan
    Molecular Parasitology Unit, Queensland Institute of Medical Research, and Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia
    Int J Infect Dis 12:143-51. 2008
    ....
  36. ncbi Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily
    S J Ackerman
    Department of Medicine, Beth Israel Hospital, Boston, MA
    J Immunol 150:456-68. 1993
    ..and sequenced a 598-bp full length cDNA clone for the human Charcot-Leyden crystal (CLC) protein (eosinophil lysophospholipase), the unique and prominent constituent of human eosinophils and basophils that forms the hexagonal ..
  37. pmc Polychlorinated biphenyls induce arachidonic acid release in human platelets in a tamoxifen sensitive manner via activation of group IVA cytosolic phospholipase A2-alpha
    Pontus K A Forsell
    Departments of Medical Biochemistry and Biophysics, Division of Medical Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Biochem Pharmacol 71:144-55. 2005
    ..We report, here, the effects of PCBs on eicosanoid formation, arachidonic acid release and cytosolic phospholipase A2-alpha (cPLA2-alpha) activation in human platelets...
  38. pmc Autotaxin/lysophospholipase D-mediated lysophosphatidic acid signaling is required to form distinctive large lysosomes in the visceral endoderm cells of the mouse yolk sac
    Seiichi Koike
    Department of Molecular Neurobiology, Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba, Ibaraki 305 8577, Japan
    J Biol Chem 284:33561-70. 2009
    Autotaxin, a lysophospholipase D encoded by the Enpp2 gene, is an exoenzyme that produces lysophosphatidic acid in the extracellular space...
  39. ncbi Purification and properties of a membrane-bound phospholipase B from Mycobacterium lepraemurium
    S Maeda
    National Institute for Leprosy Research, Tokyo, Japan
    Biochim Biophys Acta 1303:31-8. 1996
    ..and monoacyl phospholipids showing that this enzyme was classified to phospholipase B (phospholipase A1/lysophospholipase)...
  40. ncbi Serine hydrolase targets of organophosphorus toxicants
    John E Casida
    Environmental Chemistry and Toxicology Laboratory, Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720 3112, USA
    Chem Biol Interact 157:277-83. 2005
    ..is established mainly from observations with humans (butyrylcholinesterase and neuropathy target esterase-lysophospholipase) and studies with mice (cannabinoid CB1 receptor, carboxylesterase, lysophospholipase and platelet ..
  41. pmc bdhA-patD operon as a virulence determinant, revealed by a novel large-scale approach for identification of Legionella pneumophila mutants defective for amoeba infection
    P Aurass
    Robert Koch Institute, Berlin, Germany
    Appl Environ Microbiol 75:4506-15. 2009
    ..pneumophila showing phospholipase A and lysophospholipase A activities...
  42. ncbi RNA expression induced by cisplatin in an organ of Corti-derived immortalized cell line
    Maurizio Previati
    Department of Morphology and Embryology, Human Anatomy Division, Ferrara University Via Fossato di Mortara 66, 44100 Ferrara, Italy
    Hear Res 196:8-18. 2004
    ..mobilization including phospholipase A2, group IV and V, phospholipase A2 activating protein and lysophospholipase I and III, as well as lipoxygenation like arachidonate 12-lipoxygenase and arachidonate 5-lipoxygenase ..
  43. ncbi Charcot-Leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion
    Steven J Ackerman
    Department of Biochemistry and Molecular Biology, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    J Biol Chem 277:14859-68. 2002
    Charcot-Leyden crystal (CLC) protein, initially reported to possess weak lysophospholipase activity, is still considered to be the eosinophil's lysophospholipase, but it shows no sequence similarities to any known lysophospholipases...
  44. pmc Cloning and characterization of the gene encoding the major cell-associated phospholipase A of Legionella pneumophila, plaB, exhibiting hemolytic activity
    Antje Flieger
    Robert Koch Institut, Berlin Institut für Molekulare Infektionsbiologie, Julius Maximilians Universitat Wurzburg, Wurzburg, Germany
    Infect Immun 72:2648-58. 2004
    ..pneumophila possess phospholipase A activity; however, only the major secreted lysophospholipase A PlaA has been investigated on the molecular level. We therefore generated isogenic L...
  45. ncbi Acyltransferases and transacylases involved in fatty acid remodeling of phospholipids and metabolism of bioactive lipids in mammalian cells
    A Yamashita
    Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa
    J Biochem 122:1-16. 1997
    ..acyl-CoA: lysophospholipid acyltransferases, CoA-dependent and CoA-independent transacylation systems and lysophospholipase/transacylase are involved in the biosynthesis of these molecular species...
  46. ncbi Cross-talk between cytosolic phospholipase A2 alpha (cPLA2 alpha) and secretory phospholipase A2 (sPLA2) in hydrogen peroxide-induced arachidonic acid release in murine mesangial cells: sPLA2 regulates cPLA2 alpha activity that is responsible for arachido
    Won K Han
    Medical Services, Massachusetts General Hospital, Department of Medicine and Anesthesia, Harvard Medical School, Massachusetts, USA
    J Biol Chem 278:24153-63. 2003
    ..Group IIa and V PLA2s are regulatory and cPLA2alpha is responsible for AA release...
  47. doi Influence of lysophospholipid hydrolysis by the catalytic domain of neuropathy target esterase on the fluidity of bilayer lipid membranes
    Aaron J Greiner
    Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA
    Biochim Biophys Acta 1798:1533-9. 2010
    ..In vitro, NTE has phospholipase/lysophospholipase activity that hydrolyses exogenously added single-chain lysophospholipids in preference to dual-chain ..
  48. pmc Characterization of the gene encoding the major secreted lysophospholipase A of Legionella pneumophila and its role in detoxification of lysophosphatidylcholine
    Antje Flieger
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, Germany
    Infect Immun 70:6094-106. 2002
    ..acids from lysophosphatidylcholine and lysophosphatidylglycerol, implying that PlaA is the major secreted lysophospholipase A of L. pneumophila...
  49. pmc Identification of phospholipase B from Dictyostelium discoideum reveals a new lipase family present in mammals, flies and nematodes, but not yeast
    Clive P Morgan
    Department of Physiology, Rockefeller Building, University College London, University St, London WC1E 6JJ, UK
    Biochem J 382:441-9. 2004
    The social amoeba Dictyostelium discoideum exhibits high activities of phospholipase and lysophospholipase [Ferber, Munder, Fischer and Gerisch (1970) Eur. J. Biochem. 14, 253-257]...
  50. doi Maternal exposure to anti-androgenic compounds, vinclozolin, flutamide and procymidone, has no effects on spermatogenesis and DNA methylation in male rats of subsequent generations
    Kunifumi Inawaka
    Environmental Health Science Laboratory, Sumitomo Chemical Co, Ltd, Osaka, Japan
    Toxicol Appl Pharmacol 237:178-87. 2009
    ..postnatal day (PND) 6 for DNA methylation analysis of the region (210 bp including 7 CpG sites) within the lysophospholipase gene by bisulfite DNA sequencing method...
  51. ncbi Crystal structure of Escherichia coli thioesterase I/protease I/lysophospholipase L1: consensus sequence blocks constitute the catalytic center of SGNH-hydrolases through a conserved hydrogen bond network
    Yu Chih Lo
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Neihu 114, Taiwan
    J Mol Biol 330:539-51. 2003
    ..is a multifunctional enzyme possessing activities of thioesterase, esterase, arylesterase, protease, and lysophospholipase. In particular, TAP has stereoselectivity for amino acid derivative substrates, hence it is useful for the ..
  52. doi Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures
    Sanjeev Kumar Chandrayan
    Protein Science and Engineering Division, Institute of Microbial Technology IMTECH, Sector 39A, Chandigarh 160036, India
    Protein Expr Purif 59:327-33. 2008
    A putative lysophospholipase (PF0480) encoded by the Pyrococcus furiosus genome has previously been cloned and expressed in Escherichia coli...
  53. ncbi Ultrastructural localization of Charcot-Leyden crystal protein (lysophospholipase) and peroxidase in macrophages, eosinophils, and extracellular matrix of the skin in the hypereosinophilic syndrome
    A M Dvorak
    Department of Pathology, Beth Israel Hospital, Boston, Massachusetts
    Lab Invest 62:590-607. 1990
    ..abstract truncated at 400 words)..
  54. ncbi Pyrularia thionin increases arachidonate liberation and prolactin and growth hormone release from anterior pituitary cells
    A M Judd
    Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908
    Toxicon 30:1563-73. 1992
    ..increases stearate liberation from the pituitary cells, either diacylglycerol lipase, phospholipase A1 or lysophospholipase may be directly or indirectly activated by this toxin...
  55. ncbi Isolation and characterization of Escherichia coli K-12 mutants lacking both 2-acyl-glycerophosphoethanolamine acyltransferase and acyl-acyl carrier protein synthetase activity
    L Hsu
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105
    J Biol Chem 266:13783-8. 1991
    ..Acylphosphatidylglycerol accumulation was due to the transacylase activity of lysophospholipase L2 (the pldB gene product) since aas pldB double mutants accumulated 2-acyl-GPE, but not ..
  56. ncbi The catabolism of exogenous lysophosphatidylcholine in isolated perfused rat and guinea pig hearts: a comparative study
    T Mock
    Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada
    Biochim Biophys Acta 1084:167-72. 1991
    ..in lysoPC clearance by acylation catalyzed by acyl-CoA:lysoPC acyltransferase and deacylation catalyzed by lysophospholipase, respectively...
  57. doi Effect of inhibition of neuropathy target esterase in mouse nervous tissues in vitro on phosphatidylcholine and lysophosphatidylcholine homeostasis
    Wei Yuan Hou
    Institute of Zoology, CAS, Beijing 100101, PR China
    Int J Toxicol 28:417-24. 2009
    Neuropathy target esterase has been shown to be a lysophospholipase in mouse...
  58. ncbi Activated neutrophil-mediated sickle red blood cell adhesion to lung vascular endothelium: role of phosphatidylserine-exposed sickle red blood cells
    Johnson Haynes
    Jr, USA Comprehensive Sickle Cell Center, Univ of South Alabama Medical Center, 2451 Fillingim St, MCSB 1530, Mobile, AL 36617, USA
    Am J Physiol Heart Circ Physiol 291:H1679-85. 2006
    ..Zileuton attenuation of AN-mediated SRBC PS externalization suggests that a 5-lipoxygenase product(s), secreted by the AN, plays a vital role in altering the adhesive properties of PS-exposed SRBCs to vascular endothelium...
  59. doi The homeostasis of phosphatidylcholine and lysophosphatidylcholine was not disrupted during tri-o-cresyl phosphate-induced delayed neurotoxicity in hens
    Wei Yuan Hou
    Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datunlu Road, Beijing 100101, PR China
    Toxicology 252:56-63. 2008
    ..of OPIDN because new clues to possible mechanisms of OPIDN have recently been discovered that NTE acts as lysophospholipase (LysoPLA) in mice and phospholipase B (PLB) in cultured mammalian cells...
  60. ncbi An enzymatic assay for lysophosphatidylcholine concentration in human serum and plasma
    Tatsuya Kishimoto
    Diagnostic Research and Development Dept, R and D Division, Nesco Company, Azwell Inc, Osaka, Japan
    Clin Biochem 35:411-6. 2002
    ..However, these methods are not practical because they are either too complicated and/or too time-consuming for LPC determinations in human serum and plasma...
  61. ncbi Peritoneal fluids from patients with certain gynecologic tumor contain elevated levels of bioactive lysophospholipase D activity
    Akira Tokumura
    Department of Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1 78 1 Shomachi, Tokushima, Japan
    Life Sci 80:1641-9. 2007
    ..Although LPA in human serum seems mainly to be generated by lysophospholipase D (lysoPLD), the source and pathway for LPA in the ascitic fluid remain still obscure...
  62. ncbi The PLB2 gene of Saccharomyces cerevisiae confers resistance to lysophosphatidylcholine and encodes a phospholipase B/lysophospholipase
    H Fyrst
    Children s Hospital Oakland Research Institute, California 94609 1809, USA
    Biochemistry 38:5864-71. 1999
    The PLB1 gene of Saccharomyces cerevisiae encodes a protein that demonstrates phospholipase B, lysophospholipase, and transacylase activities. Several genes with significant homology to PLB1 exist in the S...
  63. ncbi Biochemical and molecular characterization of a novel choline-specific glycerophosphodiester phosphodiesterase belonging to the nucleotide pyrophosphatase/phosphodiesterase family
    Hideki Sakagami
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
    J Biol Chem 280:23084-93. 2005
    ..Previously we showed that lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) to produce lysophosphatidic acid, is identical to NPP2...
  64. ncbi Structure of human milk bile salt activated lipase
    T Baba
    Oklahoma Medical Research Foundation, University of Oklahoma Health Science Center, Oklahoma City 73104
    Biochemistry 30:500-10. 1991
    ..The amino acid sequence of human BAL is related to that of pancreatic lysophospholipase, cholesterol esterase, cholinesterase, acetylcholinesterase, and thyroglobulin...
  65. ncbi Characterization of the transacylase activity of rat liver 60-kDa lysophospholipase-transacylase. Acyl transfer from the sn-2 to the sn-1 position
    H Sugimoto
    Department of Biochemistry, Gunma University School of Medicine, Maebashi 371 8511, Japan
    Biochim Biophys Acta 1438:264-72. 1999
    Rat liver 60-kDa lysophospholipase-transacylase catalyzes not only the hydrolysis of 1-acyl-sn-glycero-3-phosphocholine, but also the transfer of its acyl chain to a second molecule of 1-acyl-sn-glycero-3-phosphocholine to form ..
  66. ncbi Molecular cloning and expression of cDNA for rat pancreatic cholesterol esterase
    J A Kissel
    Department of Pathology, University of Cincinnati, College of Medicine, OH 45267 0529
    Biochim Biophys Acta 1006:227-36. 1989
    ..with other proteins revealed that the pancreatic cholesterol esterase is identical to rat pancreatic lysophospholipase. The primary structure of cholesterol esterase displayed no significant homology with other lipases, ..
  67. ncbi Functional analyses of placental protein 13/galectin-13
    Nandor G Than
    First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary
    Eur J Biochem 271:1065-78. 2004
    ..Similar to human eosinophil Charcot-Leyden crystal protein/galectin-10 but not other galectins, its weak lysophospholipase activity was confirmed by 31P-NMR...
  68. pmc Restoration of on-time embryo implantation corrects the timing of parturition in cytosolic phospholipase A2 group IVA deficient mice
    Naoko Brown
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Biol Reprod 81:1131-8. 2009
    b>Cytosolic phospholipase A2 (cPLA2, PLA2G4A) catalyzes the release of arachidonic acid for prostaglandin synthesis by cyclooxygenase 1 (PTGS1) and cyclooxygenase 2 (PTGS2)...
  69. ncbi Secretion and lysophospholipase D activity of autotaxin by adipocytes are controlled by N-glycosylation and signal peptidase
    Jean Philippe Pradere
    INSERM, U586, Unité de Recherches sur les Obésités, Toulouse, F 31432, France
    Biochim Biophys Acta 1771:93-102. 2007
    Autotaxin (ATX) is a lysophospholipase D involved in synthesis of lysophosphatidic acid (LPA). ATX is secreted by adipocytes and is associated with adipogenesis and obesity-associated diabetes...
  70. ncbi Structural characterization of a biologically active human lipocortin 1 expressed in Escherichia coli
    R Arcone
    CEINGE, Biotecnologie Avanzate, Napoli, Italy
    Eur J Biochem 211:347-55. 1993
    ..Recombinant human lipocortin 1 reduces the carrageenin-induced paw oedema in rat in vivo and inhibits porcine pancreatic phospholipase A2 activity in vitro; in both cases, a dose-related response is observed...
  71. ncbi Purification and characterization of bovine pancreatic bile salt-activated lipase
    H Tanaka
    Technology and Research Institute, Snow Brand Milk Products Co Ltd, Kawagoe, 350 1165, Japan
    J Biochem 125:883-90. 1999
    ..Previously from bovine pancreas a lysophospholipase has been purified and a gene was cloned and sequenced encoding an enzyme with cholesterol esterase/..
  72. pmc Autotaxin is released from adipocytes, catalyzes lysophosphatidic acid synthesis, and activates preadipocyte proliferation. Up-regulated expression with adipocyte differentiation and obesity
    Gilles Ferry
    Institut de Recherche Servier, Centre de Recherche de Croissy, 78290 Croissy sur Seine, France
    J Biol Chem 278:18162-9. 2003
    ..S. (2002) J. Lipid Res. 43, 904-910) the presence, in adipocyte conditioned-medium, of a soluble lysophospholipase d-activity (LPLDact) involved in synthesis of the bioactive phospholipid lysophosphatidic acid (LPA)...
  73. pmc Legionella pneumophila type II protein secretion promotes virulence in the A/J mouse model of Legionnaires' disease pneumonia
    Ombeline Rossier
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 72:310-21. 2004
    ..that the type II system controls the secretion of protease, RNase, lipase, phospholipase A, phospholipase C, lysophospholipase A, and tartrate-sensitive and tartrate-resistant acid phosphatase activities and influences the appearance ..
  74. ncbi Combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
    Mark A Kwatia
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607 7170, USA
    J Allergy Clin Immunol 119:838-47. 2007
    ..Phosphatidylcholine, the principal component of pulmonary surfactant that maintains small airway patency, is hydrolyzed by sPLA(2). The lysophosphatidylcholine product is the substrate for eosinophil lysophospholipases...
  75. doi Acyl-CoA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis
    Wei Gao
    School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Plant J 62:989-1003. 2010
    ..Arabidopsis thaliana lysophospholipase 2 (lysoPL2) (At1g52760) was identified as a protein interactor of acyl-CoA-binding protein 2 (ACBP2) in ..
  76. ncbi Charcot-Leyden crystal protein in the degranulation and recovery of activated basophils
    L M Golightly
    Department of Medicine, Beth Israel Hospital, Boston, Massachusetts
    J Leukoc Biol 51:386-92. 1992
    ..crystal (CLC) protein, a prominent cell constituent unique to eosinophils and basophils, possesses lysophospholipase activity...
  77. ncbi Purification and characterization of a catalytic domain of rat intestinal phospholipase B/lipase associated with brush border membranes
    H Tojo
    Department of Molecular Physiological Chemistry, Osaka University Medical School, Japan
    J Biol Chem 273:2214-21. 1998
    ..The purified enzyme exhibited broad substrate specificity including esterase, phospholipase A2, lysophospholipase, and lipase activities...
  78. pmc Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids
    Karen Côtes
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025 CNRS, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Biochem J 408:417-27. 2007
    The Rv0183 gene of the Mycobacterium tuberculosis H37Rv strain, which has been implicated as a lysophospholipase, was cloned and expressed in Escherichia coli...
  79. doi The homeostasis of phosphatidylcholine and lysophosphatidylcholine in nervous tissues of mice was not disrupted after administration of tri-o-cresyl phosphate
    Wei Yuan Hou
    Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China
    Toxicol Sci 109:276-85. 2009
    Neuropathy target esterase (NTE) is proven to act as a lysophospholipase (LysoPLA) in mice and phospholipase B (PLB) in cultured mammalian cells...
  80. doi Purification and characterization of lysophospholipase C from pig brain
    Nguyen Dang Hung
    College of Pharmacy, Chungnam National University, Yuseong Ku, Taejon 305 764, Korea
    Neurochem Res 35:50-9. 2010
    In present study, lysophospholipase C (lysoPLC) was purified from homogenate of pig brain...
  81. ncbi Lysophospholipase activity of Mycobacterium smegmatis
    R A Harris
    Department of Microbiology, Southeastern College of Osteopathic Medicine, North Miami Beach, Florida 33162 3097
    Zentralbl Bakteriol Mikrobiol Hyg A 264:294-300. 1987
    b>Lysophospholipase (LPL) activity found in Mycobacterium smegmatis was characterized. LPL activity was found to exist in high levels in two-to-10-day-old cultures. The enzyme assay was run using 1...
  82. ncbi Lysophospholipase L1 from Escherichia coli K-12 overproducer
    K Karasawa
    Faculty of Pharmaceutical Sciences, University of Tokyo
    J Biochem 109:288-93. 1991
    After screening 900 E. coli strains of the Clarke and Carbon collection for by lysophospholipase L1 activities, we isolated a clone bearing the plasmid pLC6-34, which showed an increased level of lysophospholipase L1 activity...
  83. pmc Synthesis and secretion of the pancreatic-type carboxyl ester lipase by human endothelial cells
    F Li
    Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, OH 45267 0529, USA
    Biochem J 329:675-9. 1998
    ..The protective effect of CEL is related to its bile-salt-independent lysophospholipase activity. However, CEL hydrolysis of lysophosphatidylcholine can be inhibited by excess cholesterol...
  84. ncbi Molecular characterization of cytosolic phospholipase A2-beta
    C Song
    Small Molecule Drug Discovery Group, Genetics Institute, Cambridge, Massachusetts 02140, USA
    J Biol Chem 274:17063-7. 1999
    We have isolated a cDNA encoding a 1012-amino acid polypeptide cPLA2-beta, that has significant homology with cPLA2-alpha in both the calcium-dependent lipid binding domain as well as in the catalytic domain...
  85. ncbi A metabolic path for the degradation of lysophosphatidic acid, an inhibitor of lysophosphatidylcholine lysophospholipase, in neuronal nuclei of cerebral cortex
    R R Baker
    Department of Biochemistry, Room 5202, Medical Sciences Bldg, University of Toronto, Toronto, Ont, Canada
    Biochim Biophys Acta 1483:58-68. 2000
    ..LysoPA is an inhibitor of the nuclear lysophosphatidylcholine (lysoPC) lysophospholipase, and by preserving lysoPC levels, lysoPA boosted the nuclear production of the acyl analogue of platelet-..
  86. pmc PLIP, a novel splice variant of Tip60, interacts with group IV cytosolic phospholipase A(2), induces apoptosis, and potentiates prostaglandin production
    A M Sheridan
    Medical Services, Massachusetts General Hospital, and Department of Medicine, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Mol Cell Biol 21:4470-81. 2001
    ..Thus PLIP, a splice variant of Tip60, interacts with cPLA(2) and potentiates cPLA(2)-mediated PGE(2) production and apoptosis...
  87. pmc Evidence that mouse brain neuropathy target esterase is a lysophospholipase
    Gary B Quistad
    Environmental Chemistry and Toxicology Laboratory, Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720 3112, USA
    Proc Natl Acad Sci U S A 100:7983-7. 2003
    ..a recombinant protein preferentially hydrolyzes lysolecithin, suggesting that this enzyme may be a type of lysophospholipase (LysoPLA) with lysolecithin as its physiological substrate...
  88. ncbi Characterization of nucleotide pyrophosphatase-5 as an oligomannosidic glycoprotein in rat brain
    Yoshihide Ohe
    Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan
    Biochem Biophys Res Commun 308:719-25. 2003
    ..However, we could not detect either the NPP activity or the lysophospholipase D activity in the immunoprecipitates with antibodies to NPP-5 from rat brain membranes...
  89. ncbi Cytosolic phospholipase A2 type IVA is present in human red cells
    Donald J Macdonald
    Biochemistry Department, Victoria Infirmary, Glasgow, G42 9TY, United Kingdom
    Blood 103:3562-4. 2004
    ..We show that IVAPLA(2) is present in red cells as a 90-kDa protein...
  90. ncbi Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU
    Miki Tamura
    Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco, CA 94143 0542, USA
    Biochem Biophys Res Commun 316:323-31. 2004
    ..was mixed with secretory type PLA(2), more phospholipase activity was observed, suggesting that ExoU has lysophospholipase A (lysoPLA) activity. A significant increase in lysoPLA activity was also observed...
  91. ncbi Mechanisms of lysophosphatidic acid production
    Junken Aoki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Semin Cell Dev Biol 15:477-89. 2004
    ..In each pathway, at least two phospholipase activities are required: phospholipase A1 (PLA1)/PLA2 plus lysophospholipase D (lysoPLD) activities are involved in the first pathway and phospholipase D (PLD) plus PLA1/PLA2 activities ..
  92. pmc Cryptococcal phospholipases: a novel lysophospholipase discovered in the pathogenic fungus Cryptococcus gattii
    Lesley C Wright
    Centre for Infectious Diseases and Microbiology, University of Sydney at Westmead, Westmead, NSW 2145, Australia
    Biochem J 384:377-84. 2004
    ..A novel phospholipase (LPL1) with only LPL (lysophospholipase) and LPTA (transacylase) activities has now been characterized in C...
  93. ncbi Lysophospholipase I identified as a ghrelin deacylation enzyme in rat stomach
    Yuka Shanado
    Tsukuba Research Institute, Novartis Institutes for BioMedical Research, Tsukuba, Japan
    Biochem Biophys Res Commun 325:1487-94. 2004
    ..and expressing candidate proteins, the ghrelin deacylation enzyme in the stomach was identified as lysophospholipase I (LysoPLA I)...
  94. ncbi Proteolytic maturation and activation of autotaxin (NPP2), a secreted metastasis-enhancing lysophospholipase D
    Silvia Jansen
    Division of Biochemistry, Flanders Interuniversity Institute for Biotechnology, Faculty of Medicine, K U Leuven, B 3000 Leuven, Belgium
    J Cell Sci 118:3081-9. 2005
    ..stimulates cell proliferation, cell motility and angiogenesis, which is accounted for by its intrinsic lysophospholipase-D activity that generates the lipid mediators lysophosphatidic acid and sphingosine-1-phosphate...
  95. ncbi Autotaxin (lysoPLD/NPP2) protects fibroblasts from apoptosis through its enzymatic product, lysophosphatidic acid, utilizing albumin-bound substrate
    Jaehwi Song
    Department of Pathology, College of Medicine and Microdissection Genomics Research Center, The Catholic University of Korea, Banpo dong number 505, Seocho Gu, Seoul 137 701, Republic of Korea
    Biochem Biophys Res Commun 337:967-75. 2005
    ..multiple enzymatic activities including ATPase, Type I nucleotide pyrophosphatase/phosphodiesterase, and lysophospholipase D, depending on its substrates...
  96. ncbi Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis
    Changling Ma
    Department of Parasitology, Zhongshan School of Medicine, SunYat sen University, Guangzhou, 510080, People s Republic of China
    Parasitol Res 101:419-25. 2007
    A cDNA clone encoding a novel lysophospholipase with a predicted molecular weight of 25.2 kDa was isolated from a Clonorchis sinensis adult cDNA library...
  97. ncbi Validation of an autotaxin enzyme immunoassay in human serum samples and its application to hypoalbuminemia differentiation
    Kazuhiro Nakamura
    Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan
    Clin Chim Acta 388:51-8. 2008
    ..of lysophosphatidic acid (LPA), an important and multi-functional bioactive lipid, through its lysophospholipase D activity (lysoPLD)...
  98. ncbi Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase
    Petra C Kienesberger
    Institute of Molecular Biosciences, University of Graz 8010, Graz, Austria
    J Biol Chem 283:5908-17. 2008
    ..Here we show that the structurally related murine protein NTE-related esterase (NRE) is a potent lysophospholipase. The enzyme efficiently hydrolyzes sn-1 esters in lysophosphatidylcholine and lysophosphatidic acid...
  99. doi TLR2 ligands augment cPLA2alpha activity and lead to enhanced leukotriene release in human monocytes
    Sabine C Lindner
    Institute of Pharmaceutical Chemistry Center for Drug Research, Development, and Safety ZAFES, Goethe University, Frankfurt Main, Germany
    J Leukoc Biol 86:389-99. 2009
    ..TLR2 ligand-induced increase of AA release was blocked by a neutralizing anti-hTLR2 antibody, indicating that TLR2 mediates augmented cPLA(2) activation and subsequent LT biosynthesis...
  100. doi Increased expression and activation of cytosolic phospholipase A2 in the spinal cord of patients with sporadic amyotrophic lateral sclerosis
    Noriyuki Shibata
    Department of Pathology, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku 162 8666, Tokyo, Japan
    Acta Neuropathol 119:345-54. 2010
    Compelling evidence identifies a link between cytotoxic effects of cytosolic phospholipase A2 (cPLA2) activity and neuron death in cell cultures...
  101. doi Deficiency of autotaxin/lysophospholipase D results in head cavity formation in mouse embryos through the LPA receptor-Rho-ROCK pathway
    Seiichi Koike
    Department of Molecular Neurobiology, Graduate School of Comprehensive Human Sciences, Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8577, Japan
    Biochem Biophys Res Commun 400:66-71. 2010
    ..These results reveal the signal transduction defects that underlie the abnormalities in Enpp2(-/-) embryos...

Research Grants34

  1. Role of a Novel Lysophospholipase in Tumorigenesis
    Ying Huang; Fiscal Year: 2012
    ..We have cloned a cDNA that encodes a novel putative lysophospholipase. In view of its predicted molecular mass of 34 kDa, we have named it LPL34 (Lysophospholipase 34)...
  2. Dennis E Hallahan; Fiscal Year: 2014
    ..We have recently shown that the substrate for LysoPLD, LPC, is induced by 2 Gy x-irradiation. Cytosolic phospholipase A2 (cPLA2) is activated by 2 Gy and knock down, knock out or inhibition of cPLA2 improves the cytotoxicity ..
  3. LIPID METABOLISM IN STIMULATED HUMAN PLATELETS
    M Broekman; Fiscal Year: 1991
    ..re)synthesis of platelet and neutrophil phospholipids 2) the activity of lysophospholipase(s) in platelets and neutrophils, and how these enzymes may regulate levels of lysophospholipids, which are ..
  4. Characterization of a Novel Lysophospholipase
    Ying Huang; Fiscal Year: 2003
    ..In view of its predicted molecular mass of 34 kDa, we have named it LPL34 (lysophospholipase 34)...
  5. Susan S Smyth; Fiscal Year: 2014
    ..The secreted lysophospholipase D autotaxin (autotaxin/lysoLPD) is responsible for production of extracellular, biologically active LPA, ..
  6. Regulation of Lysolipid Signaling by Autotaxin-Integrin Interactions on Platelets
    ZACHARY FULKERSON; Fiscal Year: 2013
    ..The production of LPA in circulation is dependent tipon autotaxin (ATX), an enzyme with lysophospholipase D activity...
  7. Lysolipid Signaling in Cardiovascular Disease
    Susan S Smyth; Fiscal Year: 2013
    ..We report a novel pathway for regulation of platelet LPA responses that involves recruitment of the lysophospholipase D enzyme, autotaxin, responsible for LPA production to activated platelets...
  8. Arie Kaffman; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Autotaxin (ATX) is a secreted lysophospholipase that is essential for normal proliferation, migration, and survival of neural stem cells (NSC) during embryonic development...
  9. Gabor J Tigyi; Fiscal Year: 2016
    ..ATX, a lysophospholipase D that generates LPA, is among the 40 most upregulated genes in metastatic cancers...
  10. ERYTHROCYTE METABOLISM IN MUSCULAR DISEASE
    Akira Omachi; Fiscal Year: 1980
    ..LDC concentrations that cause lysis in MMD, DMD, and control erythrocytes will also be determined. Phospholipase and lysophospholipase activites will be assessed in membrane preparations from MMD, DMD, and control erythrocytes.
  11. BIOCHEMICAL RELATIONSHIPS OF BASOPHILS AND EOSINOPHILS
    Steven Ackerman; Fiscal Year: 1990
    ..of the subcellular localization, enzymatic activity, uptake and secretion of Charcot-Leyden crystal protein (lysophospholipase), eosinophil granule major basic protein and eosinophil peroxidase in the basophil will utilize ..
  12. REGULATION AND FUNCTION OF CPLA2 IN UV INJURY
    Alice Pentland; Fiscal Year: 2000
    ..Recent work in our laboratory suggests that cytosolic phospholipase A2 (cPLA2) is an active participant in this process...
  13. CHARACTERIZATION OF PHOSPHOLIPASES FROM VENOM
    Robert Noble; Fiscal Year: 1980
    ..In contrast, phospholipase B (PLB; lysophospholipase) has not been studied due to the lack of availability of large amounts of purified enzyme...
  14. MOLECULAR PHARMACOLOGY OF LYSOPHOSPHATIDIC ACID
    Kevin Lynch; Fiscal Year: 2006
    ..an interaction strengthened by the molecular definition of target proteins including the LPA receptors, lysophospholipase D and lipid phosphohydrolases...
  15. Workshop on Molecular Aspects of Myeloid Stem Cell Development and Leukemia
    Steven Ackerman; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  16. GABAERGIC INSECTICIDE TOXICOLOGY
    JOHN CASIDA; Fiscal Year: 2002
    ....
  17. SQUALENE AND OXIDOSQUALENE CYCLASES
    Glenn Prestwich; Fiscal Year: 2002
    ....
  18. Third Biennial International Eosinophil Symposium 2003
    Steven Ackerman; Fiscal Year: 2003
    ..abstract_text> ..
  19. Liposome Overlays for Protein-Ligand Selectivity
    Colin Ferguson; Fiscal Year: 2004
    ..Finally we will develop PolyPIPosomes TM and PIPosomes TM research products and establish collaborations for beta-testing products to facilitate their introduction to the market. ..
  20. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Andrew Morris; Fiscal Year: 2004
    ..For the past 10 years this field has generated about 2000 peer reviewed research publications each year. The funds requested in this proposal will make an invaluable contribution to the support of this conference. ..
  21. Airway Inflammation: Bacterial Epithelial Interactions
    BRYAN HURLEY; Fiscal Year: 2004
    ..Results obtained from the following proposal may shed light on diseases involving bacterial mediated lung inflammation such as cystic fibrosis and should also provide new strategies directed towards treating lung disease. ..
  22. Computational Approach to Ligand Discovery for LPA GPCR and PPAR
    Abby L Parrill; Fiscal Year: 2010
    ..This research can therefore lead to new treatments for cardiovascular disease and cancer. ..
  23. Thermo/Finnigan TSQ AM Triple Stage Quadrupole Mass Spectrometer
    Andrew Morris; Fiscal Year: 2008
    ..Acquisition of this instrument would allow this group of investigators to complete their current and future research projects far more efficiently to generate optimal research outcomes. [unreadable] [unreadable] [unreadable]..
  24. Crosslinkable Hydrogels for Tympanic Membrane Repair
    Glenn Prestwich; Fiscal Year: 2004
    ..Third, we will evaluate the biocompability and otocompability of each gel in vivo by evaluating recovery of auditory function and middle ear histology. ..
  25. Phosphoinositide and Inositol Polyphosphate Probes
    Glenn Prestwich; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  26. Lysophospholipid Mediators in Ovarian Cancer
    Andrew Morris; Fiscal Year: 2005
    ..Successful completion of the research will provide the framework and impetus to develop novel effective therapeutics for ovarian cancer. ..
  27. Organophosphorus Pesticide Toxicology
    JOHN CASIDA; Fiscal Year: 2006
    ..of toxicity from disruption of signal transduction pathways, and more specifically those mediated by lysophospholipase, diacylglycerol lipase, and the muscarinic acetylcholine receptor...
  28. Molecular Aspects of Myeloid Development and Leukemia
    Steven Ackerman; Fiscal Year: 2005
    ..abstract_text> ..
  29. FASEB SUMMER RESEARCH CONFERENCE: BIOACTIVE LYSOLIPIDS
    Andrew Morris; Fiscal Year: 2005
    ..abstract_text> ..
  30. Fluorogenic Assays for Measuring the Activity of Ceramidase and Sphingomyelinase
    Colin Ferguson; Fiscal Year: 2008
    ..These assays will help to further basic research in this growing field and potentially be used for developing new drugs for diseases such as cancer, diabetes, and Alzheimer's disease. [unreadable] [unreadable] [unreadable]..
  31. CHOLINERGIC INSECTICIDE TOXICOLOGY
    JOHN CASIDA; Fiscal Year: 2006
    ....
  32. MOLECULAR BIOLOGY AND FUNCTIONS OF EOSINOPHIL PROTEINS
    Steven Ackerman; Fiscal Year: 2005
    ..CLC protein was originally identified as eosinophil lysophospholipase (LPLase), but we have now shown it to be a member of the galectin superfamily of animal lectins based on ..
  33. CYTOKINE REGULATION OF HUMAN EOSINOPHIL GENES
    Steven Ackerman; Fiscal Year: 2006
    ....
  34. Thiol-Reactive Crosslinkers for Biomaterials
    Glenn Prestwich; Fiscal Year: 2004
    ..Fourth, these new materials will be evaluated for cytocompatibility and cell growth in vitro with murine fibroblasts. Finally, selected materials will be seeded with fibroblasts and injected into nude mice for in vivo tissue generation. ..