PKN3

Summary

Gene Symbol: PKN3
Description: protein kinase N3
Alias: UTDP4-1, serine/threonine-protein kinase N3, protein kinase PKN-beta, protein-kinase C-related kinase 3
Species: human

Top Publications

  1. ncbi Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha
    K Oishi
    Graduate School of Science and Technology, Faculty of Science, Kobe, 657 8501, Japan
    Biochem Biophys Res Commun 261:808-14. 1999
  2. pmc PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase
    Frauke Leenders
    Atugen AG, Berlin, Germany
    EMBO J 23:3303-13. 2004
  3. ncbi PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family
    H Shibata
    Department of Biology, Faculty of Science, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    J Biochem 130:23-31. 2001
  4. doi PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts
    Sally Danno
    Graduate School of Medicine, Kobe University, Kobe, 650 0017, Japan
    Genes Cells . 2017
  5. ncbi Identification of a putative target for Rho as the serine-threonine kinase protein kinase N
    M Amano
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    Science 271:648-50. 1996
  6. ncbi First-in-human phase I study of Atu027, a liposomal small interfering RNA formulation, targeting protein kinase N3 (PKN3) in patients with advanced solid tumors
    D Strumberg
    Marienhospital Herne, Herne, Germany University of Bochum, Marienhospital Herne, Herne, Germany Silence Therapeutics AG, Berlin, Germany Gemeinschaftskrankenhaus Herdecke, Herdecke, Germany
    J Clin Oncol 29:3057. 2011
  7. pmc Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors
    Matthew D Falk
    Pfizer Oncology Research Unit, Pfizer Inc, San Diego, CA 92121, U S A
    Biosci Rep 34:. 2014
  8. pmc PKN3 is the major regulator of angiogenesis and tumor metastasis in mice
    Hideyuki Mukai
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Sci Rep 6:18979. 2016
  9. pmc Identification of Host Kinase Genes Required for Influenza Virus Replication and the Regulatory Role of MicroRNAs
    Abhijeet Bakre
    Department of Infectious Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 8:e66796. 2013
  10. doi The interaction of PKN3 with RhoC promotes malignant growth
    Keziban Unsal-Kaçmaz
    Oncology Research Unit, Pfizer Oncology, Pfizer Worldwide Research and Development, Pearl River, NY 10965, USA
    Mol Oncol 6:284-98. 2012

Scientific Experts

  • Hideyuki Mukai
  • Sally Danno
  • Kristin Möpert
  • Keziban Unsal-Kaçmaz
  • Alejandra Collazos
  • Anke Klippel
  • Ansgar Santel
  • Jörg Kaufmann
  • Koji Kubouchi
  • Mona Mehruba
  • K Oishi
  • Manuela Aleku
  • Matthew D Falk
  • Abhijeet Bakre
  • Sylvie Lachmann
  • Peter J Parker
  • D Strumberg
  • Nadine Röder
  • Norma J Nowak
  • Kathrin Löffler
  • H Mukai
  • Wolfgang Arnold
  • Klaus Giese
  • Sibylle Dames
  • H Shibata
  • Frauke Leenders
  • Y Ono
  • Masahiro Oka
  • Kenji Sakimura
  • Manabu Abe
  • Hiroki Yasuda
  • Rie Natsume
  • Masanori Hirashima
  • Satoshi Eguchi
  • Go Shioi
  • Yasunori Kanaho
  • Ryosuke Satoh
  • Shozo Nishida
  • Aiko Muramatsu
  • Tsunaki Hongu
  • Rana Mashud
  • Reiko Sugiura
  • Masanobu Tsubaki
  • Sho Tsujimoto
  • Ben Bolaños
  • Stephan Grant
  • Alexei Brooun
  • Wei Liu
  • Sergei Timofeevski
  • Jackelyn Crabtree
  • Victoria Meliopoulos
  • S Mark Tompkins
  • Paula Brooks
  • Keegan Coleman
  • Xiuzhen Yan
  • Ralph A Tripp
  • Lauren E Andersen
  • Stephen Dann
  • Shoba Ragunathan
  • Erik Upeslacis
  • Richard Hernandez
  • Mary Grillo
  • Fiona Mack
  • Edward Rosfjord
  • Amy Jevons
  • Richard D H Whelan
  • Nick Totty
  • Manu De Rycker
  • C Vank
  • U Traugott
  • A Santel
  • Adele Casamassima
  • J Drevs
  • B Schultheis
  • Harry Mellor
  • K Giese
  • O Keil
  • Leon C H Pang
  • Simone Radtke
  • J Kaufmann
  • Gavin Kelly
  • Nicholas Michael
  • Sheila N J Sait
  • Dan Gaile
  • Eunice S Wang
  • Meir Wetzler
  • Song Liu
  • Amer Zeidan
  • M Takahashi
  • Paul K Wallace

Detail Information

Publications17

  1. ncbi Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha
    K Oishi
    Graduate School of Science and Technology, Faculty of Science, Kobe, 657 8501, Japan
    Biochem Biophys Res Commun 261:808-14. 1999
    ..The identification of this novel isoform underscores the diversity of PKN signaling pathway...
  2. pmc PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase
    Frauke Leenders
    Atugen AG, Berlin, Germany
    EMBO J 23:3303-13. 2004
    ..Using this approach we identified and validated PKN3, a barely characterized protein kinase C-related molecule, as a novel effector mediating malignant cell growth ..
  3. ncbi PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family
    H Shibata
    Department of Biology, Faculty of Science, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    J Biochem 130:23-31. 2001
    ..The catalytically active form of PKNbeta phosphorylated Graf and Graf2 in vitro. The interplay of PKNbeta and the GTPase-activating proteins, Graf and Graf2, may offer a novel mechanism regulating the Rho-mediated signaling...
  4. doi PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts
    Sally Danno
    Graduate School of Medicine, Kobe University, Kobe, 650 0017, Japan
    Genes Cells . 2017
    ..Comparison of the PKN2-/- phenotype with the phenotypes of PKN1 and PKN3 knockout strains suggests that PKN2 has distinct nonredundant functions in vivo, despite the structural similarity ..
  5. ncbi Identification of a putative target for Rho as the serine-threonine kinase protein kinase N
    M Amano
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    Science 271:648-50. 1996
    ..PKN was phosphorylated in Swiss 3T3 cells stimulated with LPA, and this phosphorylation was blocked by treatment of cells with botulinum C3 exoenzyme. Activation of Rho may be linked directly to a serine-threonine kinase pathway...
  6. ncbi First-in-human phase I study of Atu027, a liposomal small interfering RNA formulation, targeting protein kinase N3 (PKN3) in patients with advanced solid tumors
    D Strumberg
    Marienhospital Herne, Herne, Germany University of Bochum, Marienhospital Herne, Herne, Germany Silence Therapeutics AG, Berlin, Germany Gemeinschaftskrankenhaus Herdecke, Herdecke, Germany
    J Clin Oncol 29:3057. 2011
    ..is a novel RNAi therapeutic based on cationic lipoplexes containing chemically stabilized siRNAs, which targets PKN3 gene expression in the vascular endothelium...
  7. pmc Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors
    Matthew D Falk
    Pfizer Oncology Research Unit, Pfizer Inc, San Diego, CA 92121, U S A
    Biosci Rep 34:. 2014
    ..Inhibition of tumour PKN activity has been explored as an oncology therapeutic approach, with a PKN3-targeted RNAi (RNA interference)-derived therapeutic agent in Phase I clinical trials...
  8. pmc PKN3 is the major regulator of angiogenesis and tumor metastasis in mice
    Hideyuki Mukai
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Sci Rep 6:18979. 2016
    ..Furthermore, the enrichment of PKN3 mRNA in some cancer cell lines as well as its requirement in malignant prostate cell growth suggested its ..
  9. pmc Identification of Host Kinase Genes Required for Influenza Virus Replication and the Regulatory Role of MicroRNAs
    Abhijeet Bakre
    Department of Infectious Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 8:e66796. 2013
    ..HPKs (NPR2, MAP3K1, DYRK3, EPHA6, TPK1, PDK2, EXOSC10, NEK8, PLK4, SGK3, NEK3, PANK4, ITPKB, CDC2L5 (CDK13), CALM2, PKN3, and HK2) were validated as essential for A/WSN/33 influenza virus replication, and 6 HPKs (CDK13, HK2, NEK8, PANK4,..
  10. doi The interaction of PKN3 with RhoC promotes malignant growth
    Keziban Unsal-Kaçmaz
    Oncology Research Unit, Pfizer Oncology, Pfizer Worldwide Research and Development, Pearl River, NY 10965, USA
    Mol Oncol 6:284-98. 2012
    b>PKN3 is an AGC-family protein kinase implicated in growth of metastatic prostate cancer cells with phosphoinositide 3-kinase pathway deregulation...
  11. doi Depletion of protein kinase N3 (PKN3) impairs actin and adherens junctions dynamics and attenuates endothelial cell activation
    Kristin Möpert
    Silence Therapeutics AG, 13125 Berlin, Germany
    Eur J Cell Biol 91:694-705. 2012
    ..Here we report that protein kinase N3 (PKN3) appears to play a pivotal role in maintaining endothelial cell morphology, cell-cell junctions and motility...
  12. ncbi Purification and kinase assay of PKN
    Hideyuki Mukai
    Biosignal Research Center, Kobe University, Japan
    Methods Enzymol 406:234-50. 2006
    ..PKN has three isoforms each derived from different genes, PKN1 (PKNalpha/PRK1/PAK1), PKN2 (PRK2/PAK2/PKNgamma), and PKN3 (PKNbeta)...
  13. pmc Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration
    Sylvie Lachmann
    Protein Phosphorylation Laboratory, London Research Institute, London, United Kingdom
    PLoS ONE 6:e21732. 2011
    ..It has been reported that PKN3 is required for prostate tumour cell invasion but not PKN1 or 2...
  14. doi Site recognition and substrate screens for PKN family proteins
    Alejandra Collazos
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 438:535-43. 2011
    ..In the present study, we have re-evaluated and compared the specificity of PKN1 and PKN3 and assessed the predictive value in substrates...
  15. pmc Recurrent deletion of 9q34 in adult normal karyotype precursor B-cell acute lymphoblastic leukemia
    Norma J Nowak
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Cancer Genet Cytogenet 199:15-20. 2010
    ..The results demonstrate cryptic deletions of 9q34 involving SET, PKN3, NUP188, ABL1, and NUP214 in three of the samples...
  16. doi Atu027, a liposomal small interfering RNA formulation targeting protein kinase N3, inhibits cancer progression
    Manuela Aleku
    Silence Therapeutics AG, Berlin, Germany
    Cancer Res 68:9788-98. 2008
    ..Here we report preclinical data for Atu027, a siRNA-lipoplex directed against protein kinase N3 (PKN3), currently under development for the treatment of advanced solid cancer...
  17. ncbi PKN regulates phospholipase D1 through direct interaction
    K Oishi
    Graduate School of Science and Technology, and the Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Biol Chem 276:18096-101. 2001
    ..The stimulation of PLD1 activity by PKNalpha was slightly enhanced by the addition of arachidonic acid. These results suggest that the PKN family functions as a novel intracellular player of PLD1 signaling pathway...