PKC alpha

Summary

Gene Symbol: PKC alpha
Description: protein kinase C alpha
Alias: AAG6, PKC-alpha, PKCA, PRKACA, protein kinase C alpha type, PKC-A, aging-associated gene 6
Species: human

Top Publications

  1. pmc Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A
    X Fang
    Department of Molecular Therapeutics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 97:11960-5. 2000
  2. ncbi Glycogen synthase kinase-3: properties, functions, and regulation
    A Ali
    Division of Experimental Therapeutics, Ontario Cancer Institute, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
    Chem Rev 101:2527-40. 2001
  3. ncbi Differential activation of the PI 3-kinase effectors AKT/PKB and p70 S6 kinase by compound 48/80 is mediated by PKCalpha
    Richard D Byrne
    Division of Cell and Molecular Biology, Imperial College, London SW7 2AZ, United Kingdom
    Cell Signal 19:321-9. 2007
  4. ncbi Expression and function of the HSD-3.8 gene encoding a testis-specific protein
    W Lin
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3 Tiao, Beijing 100005, People s Republic of China
    Mol Hum Reprod 7:811-8. 2001
  5. pmc D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanism
    D Communi
    Institute of Interdisciplinary Research, Free University of Brussels, Belgium
    EMBO J 16:1943-52. 1997
  6. ncbi Protein kinase A negatively modulates the nuclear accumulation of NF-ATc1 by priming for subsequent phosphorylation by glycogen synthase kinase-3
    Colleen M Sheridan
    Program in Immunology, Department of Molecular Pharmacology, Howard Hughes Medical Institute, California 94305, USA
    J Biol Chem 277:48664-76. 2002
  7. ncbi Nuclear export of NF-ATc enhanced by glycogen synthase kinase-3
    C R Beals
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA
    Science 275:1930-4. 1997
  8. ncbi Molecular mechanism of the induction of metalloproteinases 1 and 3 in human fibroblasts by basic calcium phosphate crystals. Role of calcium-dependent protein kinase C alpha
    Paul M Reuben
    Department of Medicine, University of Miami School of Medicine, Miami, Florida 33101, USA
    J Biol Chem 277:15190-8. 2002
  9. pmc Convergence of multiple signaling cascades at glycogen synthase kinase 3: Edg receptor-mediated phosphorylation and inactivation by lysophosphatidic acid through a protein kinase C-dependent intracellular pathway
    Xianjun Fang
    Department of Molecular Therapeutics, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Cell Biol 22:2099-110. 2002
  10. ncbi Phosphorylation of coronin 1B by protein kinase C regulates interaction with Arp2/3 and cell motility
    Liang Cai
    Lineberger Comprehensive Cancer Center and Department of Cell and Developmental Biology, University of North Carolina Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:31913-23. 2005

Research Grants

  1. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Awni Hannun; Fiscal Year: 2013
  2. Hepatoma derived growth factor and vascular smooth muscle cell proliferation
    Allen D Everett; Fiscal Year: 2010
  3. Jeff M Sands; Fiscal Year: 2014
  4. Jennifer D Black; Fiscal Year: 2014
  5. Invasion of C. neoformans into brain endothelial cells
    Ambrose Y Jong; Fiscal Year: 2012
  6. The Molecular Basis of Alcohol's Actions
    DAVID NIGEL JONES; Fiscal Year: 2010
  7. Debra Romberger; Fiscal Year: 2014
  8. The role of PKCepsilon in diabetic retinopathy
    Christian Rask-Madsen; Fiscal Year: 2012
  9. Epigenetics of the Retinoic Acid Paradox
    Nicoletta Sacchi; Fiscal Year: 2013
  10. MECHANISM OF DRUG TRANSPORT IN LUNG CANCER CELLS
    Sanjay Awasthi; Fiscal Year: 2011

Detail Information

Publications257 found, 100 shown here

  1. pmc Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A
    X Fang
    Department of Molecular Therapeutics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 97:11960-5. 2000
    ....
  2. ncbi Glycogen synthase kinase-3: properties, functions, and regulation
    A Ali
    Division of Experimental Therapeutics, Ontario Cancer Institute, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
    Chem Rev 101:2527-40. 2001
  3. ncbi Differential activation of the PI 3-kinase effectors AKT/PKB and p70 S6 kinase by compound 48/80 is mediated by PKCalpha
    Richard D Byrne
    Division of Cell and Molecular Biology, Imperial College, London SW7 2AZ, United Kingdom
    Cell Signal 19:321-9. 2007
    ..The latter is only activated by higher doses of c48/80 resulting in an inhibition of the c48/80 induced PKB phosphorylation, thus explaining the observed biphasic activation profile for PKB in response to this secretagogue...
  4. ncbi Expression and function of the HSD-3.8 gene encoding a testis-specific protein
    W Lin
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3 Tiao, Beijing 100005, People s Republic of China
    Mol Hum Reprod 7:811-8. 2001
    ..8 is a GTP binding protein. It was also shown to possess GTPase activity and to be phosphorylated by PKC in vitro. In conclusion, HSD-3.8 is a GTP binding protein and its activity may be regulated by phosphorylation...
  5. pmc D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanism
    D Communi
    Institute of Interdisciplinary Research, Free University of Brussels, Belgium
    EMBO J 16:1943-52. 1997
    ..In this study, direct evidence is provided for a novel regulation mechanism for Ins(1,4,5)P3 3-kinase (isoform A) in vitro and in intact cells...
  6. ncbi Protein kinase A negatively modulates the nuclear accumulation of NF-ATc1 by priming for subsequent phosphorylation by glycogen synthase kinase-3
    Colleen M Sheridan
    Program in Immunology, Department of Molecular Pharmacology, Howard Hughes Medical Institute, California 94305, USA
    J Biol Chem 277:48664-76. 2002
    ....
  7. ncbi Nuclear export of NF-ATc enhanced by glycogen synthase kinase-3
    C R Beals
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA
    Science 275:1930-4. 1997
    ..Because GSK-3 responds to signals initiated by Wnt and other ligands, NF-AT family members could be effectors of these pathways...
  8. ncbi Molecular mechanism of the induction of metalloproteinases 1 and 3 in human fibroblasts by basic calcium phosphate crystals. Role of calcium-dependent protein kinase C alpha
    Paul M Reuben
    Department of Medicine, University of Miami School of Medicine, Miami, Florida 33101, USA
    J Biol Chem 277:15190-8. 2002
    ..in human fibroblasts is dependent upon the calcium-dependent PKC signal transduction pathway and that the PKC alpha isozyme is specifically involved in the pathway...
  9. pmc Convergence of multiple signaling cascades at glycogen synthase kinase 3: Edg receptor-mediated phosphorylation and inactivation by lysophosphatidic acid through a protein kinase C-dependent intracellular pathway
    Xianjun Fang
    Department of Molecular Therapeutics, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Cell Biol 22:2099-110. 2002
    ..LPA and potentially other natural ligands primarily utilize a PKC-dependent pathway to modulate GSK-3...
  10. ncbi Phosphorylation of coronin 1B by protein kinase C regulates interaction with Arp2/3 and cell motility
    Liang Cai
    Lineberger Comprehensive Cancer Center and Department of Cell and Developmental Biology, University of North Carolina Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:31913-23. 2005
    ..Furthermore, Coronin 1B phosphorylation is responsible for a significant fraction of the effects of PMA on fibroblast motility...
  11. pmc Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge
    Liang Cai
    Lineberger Comprehensive Cancer Center and Department of Cell and Developmental Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 128:915-29. 2007
    ..We conclude that Coronin 1B's coordination of filament formation by Arp2/3 complex and filament turnover by Cofilin is required for effective lamellipodial protrusion and cell migration...
  12. ncbi Differential regulation of glycogen synthase kinase-3 beta by protein kinase C isotypes
    N Goode
    Imperial Cancer Research Fund, Lincoln s Inn Fields, London, United Kingdom
    J Biol Chem 267:16878-82. 1992
    ..Phosphorylation of GSK-3 beta by PKC results in its specific inactivation. These results are consistent with a model in which activation of PKC stimulates c-Jun DNA binding by inhibiting its phosphorylation by GSK-3 beta...
  13. ncbi Interaction of 14-3-3 protein with regulator of G protein signaling 7 is dynamically regulated by tumor necrosis factor-alpha
    Thomas Benzing
    Renal Division and Center for Clinical Research, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany
    J Biol Chem 277:32954-62. 2002
    ..Our findings are the first evidence that extracellular signals can modulate the activity of RGS proteins by regulating their interaction with 14-3-3...
  14. ncbi n-3 PUFAs modulate T-cell activation via protein kinase C-alpha and -epsilon and the NF-kappaB signaling pathway
    Anne Denys
    University of Burgundy, Department of Physiology, Unité Propre de Recherche et de l Enseignement Supérieur UPRES Lipids and Nutrition, Faculty of Life Sciences, Dijon 21000, France
    J Lipid Res 46:752-8. 2005
    ..Together, these results demonstrate that DHA and EPA, being released by two isoforms of phospholipase A(2), modulate IL-2 gene expression by exerting their action on two PKC isoforms and NF-kappaB in Jurkat T-cells...
  15. ncbi The MSP receptor regulates alpha6beta4 and alpha3beta1 integrins via 14-3-3 proteins in keratinocyte migration
    Massimo M Santoro
    Department of Medical Sciences, University of Piemonte Orientale A Avogadro, 28100, Novara, Italy
    Dev Cell 5:257-71. 2003
    ....
  16. doi Toll-like receptor 3 triggers apoptosis of human prostate cancer cells through a PKC-alpha-dependent mechanism
    Alessio Paone
    Department of Histology and Medical Embryology, Istituto Pasteur Fondazione Cenci Bolognetti, Sapienza University of Rome, 00161 Rome, Italy
    Carcinogenesis 29:1334-42. 2008
    ..The comprehension of the mechanisms underlying TLR3-mediated apoptosis can contribute tools to develop new agonists useful for the treatment of prostate cancer...
  17. ncbi Identification of sites phosphorylated in bovine cardiac troponin I and troponin T by protein kinase C and comparative substrate activity of synthetic peptides containing the phosphorylation sites
    T A Noland
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322
    J Biol Chem 264:20778-85. 1989
    ....
  18. ncbi Protein kinase C alpha activates RAF-1 by direct phosphorylation
    W Kolch
    Biological Research, Goedecke AG, Freiburg, Germany
    Nature 364:249-52. 1993
    ..Here we investigate the molecular mechanism and biological consequences of PKC alpha-mediated Raf-1 activation in NIH3T3 fibroblasts...
  19. ncbi Phosphorylation specificities of protein kinase C isozymes for bovine cardiac troponin I and troponin T and sites within these proteins and regulation of myofilament properties
    N M Jideama
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 271:23277-83. 1996
    ..The present study has demonstrated for the first time distinct specificities of PKC isozymes for phosphorylation of two physiological substrates in the myocardium, with functional consequences...
  20. ncbi A functional role for mitochondrial protein kinase Calpha in Bcl2 phosphorylation and suppression of apoptosis
    P P Ruvolo
    Sealy Center for Oncology and Hematology and the Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 273:25436-42. 1998
    ..Collectively, these findings identify a functional role for PKCalpha in Bcl2 phosphorylation and in resistance to chemotherapy and suggest a novel target for antileukemic strategies...
  21. ncbi Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals
    E Oancea
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 95:307-18. 1998
    ..The properties of this molecular decoding machine make PKCgamma responsive to persistent diacylglycerol increases combined with high- but not low-frequency calcium spikes...
  22. pmc Involvement of p21(Waf1/Cip1) in protein kinase C alpha-induced cell cycle progression
    A Besson
    Departments of Oncology and Clinical Neurosciences, University of Calgary, Canada
    Mol Cell Biol 20:4580-90. 2000
    ..Of the six PKC isoforms that were present in glioma cells, PKC alpha was both necessary and sufficient to promote cell cycle progression when stimulated with phorbol 12-myristate 13-..
  23. ncbi Activation of protein kinase Calpha inhibits growth of pancreatic cancer cells via p21(cip)-mediated G(1) arrest
    K M Detjen
    Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie, Universitatsklinikum Charite, Campus Virchow Klinikum, Augustenburger Platz 1, Germany
    J Cell Sci 113:3025-35. 2000
    ....
  24. ncbi Protein kinase C activation modulates alpha-calmodulin kinase II binding to NR2A subunit of N-methyl-D-aspartate receptor complex
    F Gardoni
    Institute of Pharmacological Sciences, University of Milano, Via Balzaretti 9, 20133 Milano, Italy
    J Biol Chem 276:7609-13. 2001
    ..Thus, our data provide clues on understanding the molecular basis of a direct cross-talk between alphaCaMKII and PKC pathways in the postsynaptic compartment...
  25. ncbi Sequential activation of protein kinase C (PKC)-alpha and PKC-epsilon contributes to sustained Raf/ERK1/2 activation in endothelial cells under mechanical strain
    J J Cheng
    Cardiovascular Division, Institute of Biomedical Sciences, Academia Sinica, 11529 Taipei, Taiwan, Republic of China
    J Biol Chem 276:31368-75. 2001
    ..Our findings conclude that a sequential activation of PKC isoform (alpha and epsilon) contribute to Raf/ERK1/2 activation, and PKC-epsilon appears to play a key role in endothelial adaptation to hemodynamic environment...
  26. ncbi Regulation of protein kinase C in Escherichia coli K1 invasion of human brain microvascular endothelial cells
    Sunil K Sukumaran
    Division of Infectious Diseases, Childrens Hospital Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles, California 90027, USA
    J Biol Chem 277:12253-62. 2002
    ..This is the first report of FAK- and PI3K-dependent PKC-alpha activation in bacterial invasion related to cytoskeletal reorganization...
  27. ncbi Phosphorylation of p47phox sites by PKC alpha, beta II, delta, and zeta: effect on binding to p22phox and on NADPH oxidase activation
    Alexandre Fontayne
    Institut National de la Santé et de la Recherche Médicale U 479, Centre Hospitalier Universitaire Xavier Bichat, Paris, France
    Biochemistry 41:7743-50. 2002
    ..In this study, we found that PKC isoforms known to be present in human neutrophils (PKC alpha, beta, delta, and zeta) phosphorylate p47(phox) in a time- and concentration-dependent manner, with apparent K(m)..
  28. pmc Extracellular signal-regulated kinase phosphorylates tumor necrosis factor alpha-converting enzyme at threonine 735: a potential role in regulated shedding
    Elena Diaz-Rodriguez
    Instituto de Microbiología Bioquímica and Centro de Investigación del Cáncer, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, 37007 Salamanca, Spain
    Mol Biol Cell 13:2031-44. 2002
    ....
  29. pmc Site-directed perturbation of protein kinase C- integrin interaction blocks carcinoma cell chemotaxis
    Maddy Parsons
    Richard Dimbleby Cancer Research UK Department of Cancer Research, GKT School of Medicine, St Thomas Hospital, London SE1 7EH, United Kingdom
    Mol Cell Biol 22:5897-911. 2002
    ..Protein kinase C alpha (PKC alpha) has been implicated in the promotion of a migratory cell phenotype...
  30. ncbi Alpha-tocopherol decreases superoxide anion release in human monocytes under hyperglycemic conditions via inhibition of protein kinase C-alpha
    Senthil Kumar Venugopal
    Laboratory for Atherosclerosis and Metabolic Research, Department of Pathology, UC Davis Medical Center, Sacramento, California 95817, USA
    Diabetes 51:3049-54. 2002
    ....
  31. ncbi Role of protein kinase C alpha in calcium induced keratinocyte differentiation: defective regulation in squamous cell carcinoma
    Lee Chuan Yang
    Department of Medicine, Veterans Affairs Medical Center, San Francisco, California, USA
    J Cell Physiol 195:249-59. 2003
    ..Failure to regulate PKC [alpha] in SCC4 may underlie at least part of the failure of calcium to promote differentiation in these cells...
  32. ncbi Ca2+-dependent inhibition of NHE3 requires PKC alpha which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexes
    Whaseon Lee-Kwon
    Johns Hopkins Univ School of Medicine, 925 Ross Research Bldg, 720 Rutland Ave, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 285:C1527-36. 2003
    ..These results suggest that PKCalpha is not necessary for the Ca2+-dependent formation of the NHE3 plasma membrane complex, although it is necessary for decreasing the membrane amounts of NHE3, probably by stimulating NHE3 endocytosis...
  33. ncbi Cooperation between PKC-alpha and PKC-epsilon in the regulation of JNK activation in human lung cancer cells
    Wenhua Lang
    University of Florida Shands Cancer Center, Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 1600 SW Archer Road, P O Box 100232, Gainesville, FL 32610 0232, USA
    Cell Signal 16:457-67. 2004
    ..Furthermore, using dominant negative mutants and pharmacological inhibitors, we define that a putative Rac1/Cdc42/PKC-alpha pathway is convergent with the PKC-epsilon/MEK1/2 pathway in terms of the activation of JNK by PMA...
  34. ncbi Serine and threonine phosphorylation of the low density lipoprotein receptor-related protein by protein kinase Calpha regulates endocytosis and association with adaptor molecules
    Sripriya Ranganathan
    Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 279:40536-44. 2004
    ..These results indicate that phosphorylation of LRP by PKCalpha modulates the endocytic and signaling function of LRP by modifying its association with adaptor proteins...
  35. ncbi Phosphorylation of NG2 proteoglycan by protein kinase C-alpha regulates polarized membrane distribution and cell motility
    Irwan T Makagiansar
    Cancer Research Center, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Biol Chem 279:55262-70. 2004
    ..PKC-alpha-mediated NG2 phosphorylation at Thr(2256) is therefore a key step for initiating cell polarization and motility...
  36. ncbi Activation-induced depletion of protein kinase C alpha provokes desensitization of monocytes/macrophages in sepsis
    Andreas von Knethen
    Department of Cell Biology, University Kaiserslautern, Kaiserslautern, Germany
    J Immunol 174:4960-5. 2005
    ..Inhibition of the oxidative burst and depletion of protein kinase C alpha (PKC alpha) were correlated in septic patients...
  37. ncbi Activation of protein kinase C alpha is required for TPA-triggered ERK (MAPK) signaling and growth inhibition of human hepatoma cell HepG2
    Wu Wen-Sheng
    Department of Medical Technology, Tzu Chi University, Taiwan
    J Biomed Sci 12:289-96. 2005
    ..Taken together, we concluded that PKCalpha is specifically required for TPA-induced ERK(MAPK) signaling to trigger gene expressions of p15(INK4b) and p16(INK4a) leading to HepG2 growth inhibition...
  38. ncbi The origin of C1A-C2 interdomain interactions in protein kinase Calpha
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607 7061, USA
    J Biol Chem 280:36452-63. 2005
    ..Collectively, these studies provide new structural insight into PKCalpha C1A-C2 interdomain interactions and the mechanism of lipid-mediated PKCalpha activation...
  39. pmc Neuronal ELAV proteins enhance mRNA stability by a PKCalpha-dependent pathway
    Alessia Pascale
    Department of Experimental and Applied Pharmacology, University of Pavia, Via Taramelli 14, 27100 Pavia, Italy
    Proc Natl Acad Sci U S A 102:12065-70. 2005
    ..These findings suggest a model for gene expression regulation by nELAV proteins through a PKCalpha-dependent pathway that is relevant for the cellular programs in which ARE-mediated control plays a pivotal role...
  40. pmc Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain
    John H Evans
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Boulder, CO 80309 0215, USA
    Mol Biol Cell 17:56-66. 2006
    ..Instead, targeting specificity is provided by basic residues on beta-strands 3-4, which bind to plasma membrane PIP2...
  41. ncbi Heterogeneity of cellular proliferation within transitional cell carcinoma: correlation of protein kinase C alpha/betaI expression and activity
    Vesa Aaltonen
    Department of Anatomy and Cell Biology, University of Oulu, University of Oulu, Finland
    J Histochem Cytochem 54:795-806. 2006
    ..Furthermore, these results suggest that even in carcinoma tissue, PKC expression and activity are under strict control...
  42. ncbi Glomerular clusterin is associated with PKC-alpha/beta regulation and good outcome of membranous glomerulonephritis in humans
    M P Rastaldi
    Renal Immunopathology Laboratory, Fondazione D Amico per la Ricerca sulle Malattie Renali, Nuova Nefrologia Research Association, c o San Carlo Borromeo Hospital, Milan, Italy
    Kidney Int 70:477-85. 2006
    ..Binding of clusterin to the LDL-R might offer an interpretative key for the pathogenesis of MGN in humans...
  43. ncbi Overexpression of protein kinase C alpha mRNA in human hepatocellular carcinoma: a potential marker of disease prognosis
    Trang Tiau Wu
    Department of Surgery, School of Medicine, Medical College, Chung Shan Medical University, Taichung, Taiwan
    Clin Chim Acta 382:54-8. 2007
    ..Members of the protein kinase C (PKC) isoenzyme family play a central role in the tumorigenesis of several tissues. However, little is known about subtype specific intracellular expression of PKC in human hepatocellular carcinomas...
  44. doi Overexpression of protein kinase Calpha mRNA may be an independent prognostic marker for gastric carcinoma
    Kai Yuan Lin
    Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan
    J Surg Oncol 97:538-43. 2008
    ..The aims of this study were to correlate the expression of protein kinase Calpha (PKCalpha) mRNA with clinicopathological parameters and to evaluate the significant value of PKCalpha in gastric carcinoma prognosis...
  45. doi Protein kinase C alpha (PKCalpha) regulates growth and invasion of endometrial cancer cells
    James M Haughian
    Division of Basic Reproductive Science, Department of Obstetrics and Gynecology, University of Colorado Denver School of Medicine Aurora, Colorado 80045, USA
    J Cell Physiol 220:112-8. 2009
    ....
  46. pmc ADAM-10-mediated N-cadherin cleavage is protein kinase C-alpha dependent and promotes glioblastoma cell migration
    Zachary A Kohutek
    Departments of Pathology and Molecular Physiology and Biological Physics, University of Virginia Health System, Charlottesville, VA 22908, USA
    J Neurosci 29:4605-15. 2009
    ..Together, these results suggest that N-cadherin cleavage is regulated by a PKC-alpha-ADAM-10 cascade in GBM cells and may be involved in mediating GBM cell migration...
  47. pmc Protein kinase C alpha-dependent signaling mediates endometrial cancer cell growth and tumorigenesis
    James M Haughian
    Department of Obstetrics and Gynecology, Section of Basic Reproductive Sciences, University of Colorado, Denver School of Medicine, Aurora, CO 80045, USA
    Int J Cancer 125:2556-64. 2009
    ..Thus, targeting PKCalpha may provide novel therapeutic options in endometrial tumors...
  48. doi Phosphorylation of NADPH oxidase activator 1 (NOXA1) on serine 282 by MAP kinases and on serine 172 by protein kinase C and protein kinase A prevents NOX1 hyperactivation
    Yolande Kroviarski
    INSERM U773, Centre de recherche Biomedicale Bichat Beaujon, Paris, France
    FASEB J 24:2077-92. 2010
    ..These results demonstrated a critical role of NOXA1 phosphorylation on Ser-282 and Ser-172 in preventing NOX1 hyperactivation through the decrease of NOXA1 interaction to NOX1 and Rac1...
  49. pmc PKCalpha expression is a marker for breast cancer aggressiveness
    Gry Kalstad Lønne
    Center for Molecular Pathology, Department of Laboratory Medicine, Lund University, Malmo University Hospital, SE 205 02 Malmo, Sweden
    Mol Cancer 9:76. 2010
    ..Protein kinase C (PKC) isoforms are potential targets for breast cancer therapy. This study was designed to evaluate which PKC isoforms might be optimal targets for different breast cancer subtypes...
  50. doi Up-regulation of cyclin D1 expression in asthma serum-sensitized human airway smooth muscle promotes proliferation via protein kinase C alpha
    Chun Ling Du
    Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Exp Lung Res 36:201-10. 2010
    ..The authors' previous studies have indicated that protein kinase C alpha (PKC alpha) is involved in the proliferation of passively sensitized human airway smooth muscle cells (HASMCs)...
  51. pmc Protein kinase Cα promotes cell migration through a PDZ-dependent interaction with its novel substrate discs large homolog 1 (DLG1)
    Audrey K O'Neill
    Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 286:43559-68. 2011
    ..Taken together, these data establish the requirement of scaffolding to DLG1 for PKCα to promote cellular migration...
  52. pmc Protein kinase C-α and arginase I mediate pneumolysin-induced pulmonary endothelial hyperpermeability
    Rudolf Lucas
    Vascular Biology Center and Dept of Pharmacology and Toxicology, Georgia Health Sciences University, 1459 Laney Walker Blvd, Augusta, GA 30912 2500, USA
    Am J Respir Cell Mol Biol 47:445-53. 2012
    ..These results identify PKC-α and arginase I as potential upstream and downstream therapeutic targets in PLY-induced pulmonary endothelial dysfunction...
  53. pmc PKCα is genetically linked to memory capacity in healthy subjects and to risk for posttraumatic stress disorder in genocide survivors
    Dominique J F de Quervain
    Division of Cognitive Neuroscience, Department of Psychology, University of Basel, 4055 Basel, Switzerland
    Proc Natl Acad Sci U S A 109:8746-51. 2012
    ..Our results indicate a role for PKCα in memory and suggest a genetic link between memory and the risk for PTSD...
  54. pmc Protein kinase Cα protects against multidrug resistance in human colon cancer cells
    Se Kyoung Lee
    School of Life Sciences, Kyungpook National University, Daegu 702 701, Korea
    Mol Cells 34:61-9. 2012
    ....
  55. doi Protein kinase Cα inhibitor protects against downregulation of claudin-1 during epithelial-mesenchymal transition of pancreatic cancer
    Daisuke Kyuno
    Department of Surgery, Sapporo Medical University School of Medicine, Sapporo 060 8556, Japan
    Carcinogenesis 34:1232-43. 2013
    ..Thus, PKCα inhibitors may be potential therapeutic agents against the malignancy of human pancreatic cancer cells...
  56. doi PKCα is involved in the progression of kidney carcinoma through regulating netrin-1/UNC5B signaling pathway
    Bo Zhan
    Department of Urology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang City, Liaoning Province, People s Republic of China, 110001
    Tumour Biol 34:1759-66. 2013
    ..Our data highly suggested that PMA-induced upregulation and calphostin C-induced reversion of netrin-1 and UNC5B in kidney carcinoma were accompanied by the activation of the netrin-1/UNC5B pathways...
  57. ncbi Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3':5'-monophosphate-dependent protein kinase
    A Kishimoto
    J Biol Chem 260:12492-9. 1985
    ..These results provide some clues to understanding the rationale that these kinases may show different but sometimes similar functions depending on the structure of target phosphate acceptor proteins...
  58. ncbi Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinase
    P J Woodring
    Department of Pharmacology and Cancer Research Center, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Biol Chem 272:30447-54. 1997
    ..Differential expression and regulation of the two inositol 1,4,5-trisphosphate 3-kinase isoforms provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells...
  59. ncbi 14-3-3Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells
    M V Autieri
    Department of Cardiology and Physiology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    DNA Cell Biol 18:555-64. 1999
    ..Thus, 14-3-3gamma may represent a signal transduction protein that is regulated transcriptionally and post-transcriptionally by growth factors...
  60. pmc Protein kinase C-dependent mobilization of the alpha6beta4 integrin from hemidesmosomes and its association with actin-rich cell protrusions drive the chemotactic migration of carcinoma cells
    I Rabinovitz
    Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Cell Biol 146:1147-60. 1999
    ....
  61. ncbi Selective association of protein kinase C with 14-3-3 zeta in neuronally differentiated PC12 Cells. Stimulatory and inhibitory effect of 14-3-3 zeta in vivo
    Laura Gannon-Murakami
    Department of Biology, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 277:23116-22. 2002
    ..Our results show that the association with 14-3-3 zeta has distinct effects on classical PKC and non-classical PKC activity...
  62. ncbi Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains
    Bharath Ananthanarayanan
    Department of Chemistry, University of Illinois at Chicago, 845 W Taylor Street, Chicago, IL 60607, USA
    J Biol Chem 278:46886-94. 2003
    ..These data suggest that differential activation mechanisms of PKC isoforms are determined by the DAG affinity and conformational flexibility of their C1 domains...
  63. pmc Roles of PLC-gamma2 and PKCalpha in TPA-induced apoptosis of gastric cancer cells
    Bing Zhang
    Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China
    World J Gastroenterol 9:2413-8. 2003
    ..To investigate the roles of PLCgamma2 and PKCalpha in TPA-induced apoptosis of gastric cancer cells...
  64. doi IL-1 beta induces urokinase-plasminogen activator expression and cell migration through PKC alpha, JNK1/2, and NF-kappaB in A549 cells
    Ching Yi Cheng
    Department of Pharmacology, Chang Gung University, Tao Yuan, Taiwan
    J Cell Physiol 219:183-93. 2009
    ..In this study, IL-1 beta significantly induced uPA expression and activity via PKC alpha-dependent JNK1/2 and NIK cascades, linking to IKK alpha/beta activation, p65 translocation and transcription ..
  65. pmc Reactive oxygen species-generating mitochondrial DNA mutation up-regulates hypoxia-inducible factor-1alpha gene transcription via phosphatidylinositol 3-kinase-Akt/protein kinase C/histone deacetylase pathway
    Nobuko Koshikawa
    Laboratory of Cancer Metastasis, Chiba Cancer Center Research Institute, 666 2 Nitona, Chuoh ku, Chiba 260 8717, Japan
    J Biol Chem 284:33185-94. 2009
    ..These results suggest that the ROS-generating ND6 mutation increases HIF-1alpha transcription via the PI3K-Akt/PKC/HDAC pathway, leading to HIF-1alpha protein accumulation in hypoxic tumor cells...
  66. doi The vanilloid capsaicin induces IL-6 secretion in prostate PC-3 cancer cells
    Sophie Malagarie-Cazenave
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Alcala, 28871 Alcala de Henares Madrid, Spain
    Cytokine 54:330-7. 2011
    ..These results raise the possibility that capsaicin-mediated IL-6 increase in prostate cancer PC-3 cells is regulated at least in part by TNF-α secretion and signaling pathway involving Akt, ERK and PKC-α activation...
  67. pmc Mechanisms for vascular cell adhesion molecule-1 activation of ERK1/2 during leukocyte transendothelial migration
    Hiam Abdala-Valencia
    Allergy Immunology Division, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America
    PLoS ONE 6:e26706. 2011
    ....
  68. ncbi Tumor cell-mediated induction of the stromal factor stromelysin-3 requires heterotypic cell contact-dependent activation of specific protein kinase C isoforms
    Krystel Louis
    INSERM U526, IFR50, Faculté de Médecine Pasteur, 06107 Nice, France
    J Biol Chem 280:1272-83. 2005
    ..Altogether, our data emphasize signaling changes occurring in the tumor microenvironment that may define new stromal targets for therapeutic intervention...
  69. ncbi Protein Kinase C alpha is a marker for antiestrogen resistance and is involved in the growth of tamoxifen resistant human breast cancer cells
    Lisa B Frankel
    Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, 2100 Copenhagen, Denmark
    Breast Cancer Res Treat 104:165-79. 2007
    ..Furthermore, our data suggest the potential for PKCalpha as a marker for antiestrogen resistance and as a promising therapeutic target in the treatment of tamoxifen resistant breast cancer...
  70. pmc Protein kinase C alpha-dependent phosphorylation of the mRNA-stabilizing factor HuR: implications for posttranscriptional regulation of cyclooxygenase-2
    Anke Doller
    Pharmazentrum frankfurt ZAFES, Klinikum der Johann Wolfgang Goethe Universitat, 60590 Frankfurt am Main, Germany
    Mol Biol Cell 18:2137-48. 2007
    ..protein kinase C (PKC) inhibitors and small interfering RNA approaches, we demonstrated that knockdown of PKC alpha efficiently blocked the ATP-dependent nuclear HuR export to the cytoplasm...
  71. ncbi Platelet P-selectin expression: requirement for protein kinase C, but not protein tyrosine kinase or phosphoinositide 3-kinase
    Danielle Libersan
    Montreal Heart Institute and the University of Montreal, Quebec, Canada
    Thromb Haemost 89:1016-23. 2003
    ..The novel epsilon and eta and atypical zeta, but not the conventional alpha and beta and the novel delta PKCs, may be involved in this process...
  72. ncbi Physical association and functional relationship between protein kinase C zeta and the actin cytoskeleton
    J Gomez
    Centro de Investigaciones Biologicas, Madrid, Spain
    Eur J Immunol 25:2673-8. 1995
    ..PKC isoforms have been classified into four groups: Ca(2+)-dependent conventional PKC alpha, beta I, beta II, gamma; Ca(2+)-independent, novel PKC delta, epsilon, eta, phi; atypical PKC zeta, lambda, iota ..
  73. ncbi The identification of four protein kinase C isoforms in human glioblastoma cell lines: PKC alpha, gamma, epsilon, and zeta
    H Xiao
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio
    J Neurosurg 81:734-40. 1994
    ..These data suggest that alteration in the expression of PKC-epsilon and PKC-zeta could be a factor in the conversion of normal glial cells to glioblastomas...
  74. ncbi Identification and characterization of protein kinase C zeta-immunoreactive proteins
    B G Allen
    Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada
    J Biol Chem 269:29288-98. 1994
    ..The 81.3-kDa protein copurified with PKC alpha, and the 79.4-kDa protein copurified with PKC beta and PKC gamma...
  75. pmc Extracellular ATP and UTP activation of phospholipase D is mediated by protein kinase C-epsilon in rat renal mesangial cells
    J Pfeilschifter
    Department of Pharmacology, University of Basel, Switzerland
    Br J Pharmacol 110:847-53. 1993
    ..Stimulation of phospholipase D appears to involve the PKC-epsilon isoenzyme, activated by DAG derived from phosphoinositide hydrolysis by phospholipase C...
  76. ncbi Localization of protein kinase C isozymes in rat colon
    Y H Jiang
    Faculty of Nutrition, Molecular and Cell Biology Group, Texas A and M University, College Station 77843-2471, USA
    Cell Growth Differ 6:1381-6. 1995
    ..In situ RT-PCR analysis revealed that PKC alpha, delta, epsilon, and zeta mRNAs are expressed in epithelial cells along the entire colonic crypt...
  77. ncbi Selective translocation of non-conventional protein kinase C isoenzymes by gonadotropin-releasing hormone (GnRH) in the gonadotrope-derived alpha T3-1 cell line
    M Kratzmeier
    Institute for Hormone and Fertility Research, University of Hamburg, Germany
    Mol Cell Endocrinol 118:103-11. 1996
    ..the PKC-activating phorbol esters, PMA and PDBu, caused concentration-dependent increases in the proportion of PKC alpha and PKC epsilon recovered from the particulate fraction of alpha T3-1 cells, but did not induce measurable ..
  78. ncbi Differentiation of pancreatic ductal carcinoma cells associated with selective expression of protein kinase C isoforms
    M G Franz
    Department of Surgery, University of South Florida College of Medicine, Tampa, USA
    Ann Surg Oncol 3:564-9. 1996
    ..CONCLUSIONS: TNF-alpha-induced growth arrest and differentiation in HPAC cells is associated with the selective downregulation of PKC-alpha and upregulation of PKC-gamma...
  79. pmc Protein kinase C bound to the Golgi apparatus supports the formation of constitutive transport vesicles
    P Westermann
    Department of Cell Biology, , Berlin, Federal Republic of Germany
    Biochem J 320:651-8. 1996
    ..Immunoelectron microscopy of ultrathin cryosections of HepG2 cells showed that PKC-zeta predominantly attaches to the trans-Golgi region, whereas PKC-alpha binds to the cis- and trans-Golgi area...
  80. ncbi Activation of protein kinase C (alpha, beta, and zeta) by insulin in 3T3/L1 cells. Transfection studies suggest a role for PKC-zeta in glucose transport
    G Bandyopadhyay
    J. A. Haley Veterans Hospital Research Service, and Departments of Internal Medicine and Biochemistry, University of South Florida, Tampa, Florida 33612, USA
    J Biol Chem 272:2551-8. 1997
    ..These findings suggested that: (a) insulin activates PKC-zeta, as well as PKC-alpha and beta; and (b) PKC-zeta is required for, and may contribute to, insulin effects on glucose transport in 3T3/L1 cells...
  81. ncbi Identification of protein kinase C isoenzymes in smooth muscle: partial purification and characterization of chicken gizzard PKC zeta
    O Clément-Chomienne
    Smooth Muscle Research Group, Faculty of Medicine, University of Calgary, AB, Canada
    Biochem Cell Biol 74:51-65. 1996
    ..b>PKC alpha associated with the particulate fraction in the presence of Ca2+ and was extracted by divalent cation chelators...
  82. ncbi Protein kinase C-zeta mediates angiotensin II activation of ERK1/2 in vascular smooth muscle cells
    D F Liao
    Department of Medicine, Division of Cardiology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 272:6146-50. 1997
    ..These results demonstrate an important difference in signal transduction by angII compared with PDGF and phorbol ester in VSMC, and suggest a critical role for PKC-zeta and Ras in angII stimulation of ERK1/2...
  83. ncbi PKC-lambda is the atypical protein kinase C isoform expressed by immature ventricle
    V O Rybin
    Department of Medicine, Columbia University, New York, NY 10032, USA
    Am J Physiol 272:H1636-42. 1997
    ..The results of this study further identify PKC-lambda as the atypical PKC isoform expressed by the immature ventricle...
  84. pmc Diacylglycerol generated by exogenous phospholipase C activates the mitogen-activated protein kinase pathway independent of Ras- and phorbol ester-sensitive protein kinase C: dependence on protein kinase C-zeta
    M van Dijk
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Biochem J 323:693-9. 1997
    ..The results suggest that DG generated by PC-PLC can activate the MAPK pathway independent of Ras and phorbol-ester-sensitive PKC but, instead, via PKC-zeta...
  85. ncbi Retinoic acid induced growth arrest of human breast carcinoma cells requires protein kinase C alpha expression and activity
    Y Cho
    Institute of Human Nutrition, Columbia University, New York, New York 10032, USA
    J Cell Physiol 172:306-13. 1997
    ..Retinoic acid arrested T-47D proliferation, induced PKC alpha expression and concomitantly repressed PKC zeta expression...
  86. ncbi Differential activation of PKC-delta and -zeta by alpha 1-adrenergic stimulation in human airway epithelial cells
    C M Liedtke
    Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106-4948, USA
    Am J Physiol 273:C937-43. 1997
    ..Methoxamine selectively induced a sustained increase in PKC-zeta activity but only a transient increase in PKC-delta. These results suggest that PKC-delta and -zeta mediate hormonal activation of Na-Cl-K cotransport...
  87. ncbi Antisense oligodeoxynucleotide to PKC-delta blocks alpha 1-adrenergic activation of Na-K-2Cl cotransport
    C M Liedtke
    Cystic Fibrosis Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Am J Physiol 273:C1632-40. 1997
    ..These results demonstrate the selective activation of Na-K-2Cl cotransport by cytosolic PKC-delta...
  88. ncbi Atypical protein kinase C isozyme zeta mediates carbachol-stimulated insulin secretion in RINm5F cells
    S H Tang
    Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Diabetes 47:905-12. 1998
    ..The data indicate that carbachol-stimulated insulin release in RINm5F cells is mediated to a large degree by the activation of the atypical PKC isozyme zeta...
  89. ncbi Strain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals
    O Han
    Department of Surgery, Yale University School of Medicine, New Haven 06520-8062, USA
    Am J Physiol 275:G534-41. 1998
    ..These results suggest that cyclic deformation may modulate intestinal epithelial proliferation and brush-border enzyme activity by regulating PKC and tyrosine kinase signals...
  90. ncbi The proliferative effect of vascular endothelial growth factor requires protein kinase C-alpha and protein kinase C-zeta
    M Wellner
    , Virchow Klinikum, Humboldt University, Berlin, Germany
    Arterioscler Thromb Vasc Biol 19:178-85. 1999
    ..Antisense ODN for these PKC isoforms block VEGF-induced proliferation. These findings suggest that PKC isoforms alpha and zeta are important for VEGF's angiogenic effects...
  91. ncbi Role of protein kinase C (PKC) in agonist-induced mu-opioid receptor down-regulation: II. Activation and involvement of the alpha, epsilon, and zeta isoforms of PKC
    H K Kramer
    Department of Psychiatry, New York University Medical Center, New York 10016, USA
    J Neurochem 72:594-604. 1999
    ....
  92. ncbi 1,25-dihydroxyvitamin D3 and TPA activate phospholipase D in Caco-2 cells: role of PKC-alpha
    S Khare
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Am J Physiol 276:G993-G1004. 1999
    ..Taken together, these observations indicate that PKC-alpha is intimately involved in the stimulation of PLD in Caco-2 cells by 1,25(OH)2D3 or TPA...
  93. ncbi Protein kinase C isoforms during the development of deciduomata in pregnant rats
    J C Shyu
    Department of Physiology, Chung Shan Medical and Dental College, Taichung, Taiwan, ROC
    Life Sci 64:2367-73. 1999
    ..At pregnant duration, PKC alpha was down-modulated in the deciduomata but not in the myometrium...
  94. ncbi Expression and activation of protein kinase C-zeta in eosinophils after allergen challenge
    D J Evans
    Thoracic Medicine, National Heart and Lung Institute at the Imperial College School of Medicine, London SW3 6LY, United Kingdom
    Am J Physiol 277:L233-9. 1999
    ....
  95. ncbi Changes in cardiac protein kinase C activities and isozymes in streptozotocin-induced diabetes
    X Liu
    Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R2H 2A6, Canada
    Am J Physiol 277:E798-804. 1999
    ..The results suggest that the increased myocardial PKC activity and increased protein contents of the cytosolic PKC isozymes are associated with subcellular alterations and cardiac dysfunction in the diabetic heart...
  96. ncbi The involvement of protein kinase C isoenzymes alpha, epsilon and zeta in the sensitivity to antitumor treatment and apoptosis induction
    M Spitaler
    Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Austria
    Anticancer Res 19:3969-76. 1999
    ..of cells to anticancer drugs and in the induction of apoptosis, we employed antisense oligonucleotides to PKC alpha and PKC zeta, CGP 53506, a new inhibitor of PKC alpha, and cells overexpressing PKC alpha, PKC epsilon and PKC ..
  97. ncbi Insulin stimulates NHE1 activity by sequential activation of phosphatidylinositol 3-kinase and protein kinase C zeta in human erythrocytes
    M Sauvage
    Laboratoire des Biomembranes et Messagers Cellulaires, , Orsay, France
    Eur J Biochem 267:955-62. 2000
    ..time-course, insulin also promoted both the translocation and activation of PKC zeta, but had no effect on PKC alpha. Inhibition of PtdIns 3-kinase with wortmannin prevented the activation of PKC zeta by insulin...
  98. ncbi Differential expressions of protein kinase C isozymes during proliferation and differentiation of human skeletal muscle cells in vitro
    J Boczan
    Department of Physiology and Cell Physiology, University Medical School of Debrecen, Hungary
    Acta Neuropathol 99:96-104. 2000
    ..In skeletal muscle cells we could identify five PKC isozymes (PKC alpha, -gamma, -etha, -theta and -zeta)...
  99. ncbi Protein kinase C-alpha mediates endothelial barrier dysfunction induced by TNF-alpha
    T Ferro
    Research Service, Stratton Veterans Affairs Medical Center, Albany Medical College, New York 12208, USA
    Am J Physiol Lung Cell Mol Physiol 278:L1107-17. 2000
    ..The data indicate that prolonged activation of PKC-alpha, maintained by a translation-dependent pool of PKC-alpha protein, mediates TNF-alpha-induced increases in endothelial permeability in PEM...
  100. ncbi Dopamine-induced translocation of protein kinase C isoforms visualized in renal epithelial cells
    S Nowicki
    Department of Woman and Child Health, Karolinska Institute, Astrid Lindgren Children's Hospital, S-171 76 Stockholm, Sweden
    Am J Physiol Cell Physiol 279:C1812-8. 2000
    ..In conclusion, this study visualizes in renal epithelial cells a very rapid activation of the PKC-alpha and -epsilon isoforms by the D(1) receptor subtype...
  101. ncbi Regulation of CYP11B2 gene expression by protein kinase C
    J G LeHoux
    Department of Biochemistry, Faculty of Medicine, University of Sherbrooke, Quebec, Canada
    Endocr Res 26:1027-31. 2000
    ..DAG-dependent PKC alpha and PKC epsilon, and atypical PKC zeta were detected in hamster adrenal zona glomerulosa, whereas the isoforms ..

Research Grants64

  1. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Awni Hannun; Fiscal Year: 2013
    ..Based on these observations, we hypothesize that receptor-induced sustained activation of PKCa/ssII induces the formation of this novel pericentriolar endocytic compartment in a PKC and PLD- dependent manner...
  2. Hepatoma derived growth factor and vascular smooth muscle cell proliferation
    Allen D Everett; Fiscal Year: 2010
    ..PKC serines (S) to alanine (A, loss of phosphorylation) or aspartic acid (D, phosphorylation mimic) mutations, PKCa deficient knockout mice, and phospho-specific HDGF antibodies...
  3. Jeff M Sands; Fiscal Year: 2014
    ....
  4. Jennifer D Black; Fiscal Year: 2014
    ..Our studies have identified protein kinase C a (PKCa) as a novel regulator of cyclin D1 expression in intestinal epithelial cells...
  5. Invasion of C. neoformans into brain endothelial cells
    Ambrose Y Jong; Fiscal Year: 2012
    ..Dominant-negative PKCa also inhibited yeast invasion into HBMEC...
  6. The Molecular Basis of Alcohol's Actions
    DAVID NIGEL JONES; Fiscal Year: 2010
    ..This may allow future development of novel approaches to prevent alcohols actions, and the identification of genetic markers associated with a predisposition to alcoholism. ..
  7. Debra Romberger; Fiscal Year: 2014
    ....
  8. The role of PKCepsilon in diabetic retinopathy
    Christian Rask-Madsen; Fiscal Year: 2012
    ..nitric oxide production and cell proliferation, with minor or opposite effects seen after downregulation of PKCa, (3, or 8...
  9. Epigenetics of the Retinoic Acid Paradox
    Nicoletta Sacchi; Fiscal Year: 2013
    ..pathways governed by non-RAR targets capable of interacting directly with RA, such as the Protein Kinase C alpha (PKCA) and PPAR beta/delta (PPARD) pathways...
  10. MECHANISM OF DRUG TRANSPORT IN LUNG CANCER CELLS
    Sanjay Awasthi; Fiscal Year: 2011
    ..differential sensitivity of SCLC to DOX as compared with NSCLC is related to lack of phosphorylation of Rlip by PKC alpha at T297, and that Rlip is required for the DOX-protective effects of PKC alpha...
  11. INTEGRIN FUNCTION AND SIGNALING IN CARCINOMA PROGRESSION
    Arthur M Mercurio; Fiscal Year: 2012
    ..The involvement of SOX9 in basal-like tumors and its relationship to the beta4 signature will also be assessed. ..
  12. AFAP-110 as a cSrc activator in breast cancer
    Jeffrey T Holt; Fiscal Year: 2012
    ..AFAP-110 is a substrate of PKCa. Phosphorylation by PKCa results in a conformational change in AFAP-110 that releases 'autoinhibition'and ..
  13. IL3 GROWTH FACTOR SIGNALING
    William Stratford May; Fiscal Year: 2010
    ..Dynamic (reversible) Bcl2 phosphorylation is regulated by multiple Bcl2 kinases (i.e. PKC alpha, ERK1/2 and JNK1) and a physiological Bcl2 phosphatase (PP2A)...
  14. Ceramide synthase as a therapy target in prostate cancer
    Adriana Haimovitz Friedman; Fiscal Year: 2009
    ..In this project, we propose to define the involvement of PKCa and PKC5 in TPAinduced apoptosis via activation of the ATM-CS pathway...
  15. Identifying all Meckel-like ciliopathy genes by established total exome capture a
    Friedhelm Hildebrandt; Fiscal Year: 2010
    ..Using this approach we already identified mutations in SDCCAG8, PRKACA and MAP2 as novel causes of Meckel-like ciliopathies...
  16. Respiratory syncytial virus targets rafts for penetration and immunomodulation
    HOMERO GABRIEL SAN JUAN VERGARA; Fiscal Year: 2010
    ..8% of the new cases of lower tract respiratory illness in infants. Understanding the mechanistic process of the RSV infection will help in the development of the vaccines and therapies. ..
  17. Rho Regulation of Pulmonary Vascular Permeability
    Dolly Mehta; Fiscal Year: 2013
    ..signaling mechanisms of TRPC6-induced increase in lung endothelial permeability involving the activation of PKCa and p60Src kinase and the effectors RhoA and MLCK-L isoform...
  18. Vasiliki Poulaki; Fiscal Year: 2016
    ..data establish Gaq- induced signaling as the oncogenic driver in Gaq-mutant UM cells, via a pathway that involves PKCa, PKD and the steroid receptor coactivator (SRC)-3, a multifunctional oncogenic protein with potent growth promoting ..
  19. Signaling in Thymocyte Selection
    Gerald R Crabtree; Fiscal Year: 2010
    ..receptor is mediated by signals mediated by the transcriptional activation of BIM by a Signaling pathway requiring PKCa. In contrast, BIM In contrast, transcriptional activation signaling pathway requiring positive selection appears to ..
  20. A mechanism for suppression of TNF induced endothelial dysfunction
    Arnold Johnson; Fiscal Year: 2013
    ..by TNF in vitro and in vivo, and (3) These responses to TNF are prevented by inhibition of PKC in vivo and PKCa in vitro. This proposal will test the paradigm that TNF-induced (~24...
  21. Metabolic Cellular Stress and its Regulatory Mechanism in Diabetic Embryopathy
    Peixin Yang; Fiscal Year: 2012
    ..activator, trehalose, reverses hyperglycemia-induced autophagy impairment and neural tube defects (NTDs);3) PKCa gene deletion, a p70S6K1 inhibitor, an ER chaperone (4-PBA) and overexpression of sirtuin 2 (SIRT2) histone ..
  22. Marcelo G Kazanietz; Fiscal Year: 2015
    ..neoplastic (PIN) lesions, whereas no obvious phenotype was observed in similar models that we developed for PKCa or PKCd overexpression...
  23. Steven R Houser; Fiscal Year: 2016
    ..A gene vector core will generate AAV6 vectors with novel therapeutics for testing in the pig Ml model. An administrative core will ensure data sharing and effective use of all resources. ..
  24. Structure and Function of Ocular Lacritin
    GORDON LAURIE; Fiscal Year: 2007
    ..Using different inhibitor techniques, we then determined that lacritin signals from Ga1, or Ga0 to PLC-PKCa/PLD/mTOR and PLCPKCa/ STIM1/calcineurin/Ca2+/NFATC1 to activate secretory and mitogenic responses...
  25. PDK1 and PPARdelta Signaling in Mammary Tumorigenesis
    Robert I Glazer; Fiscal Year: 2010
    ..We have reported that PDK1 and its downstream effector, PKCa, ncreased expression of the /?-cateninfi~CF target genes, cyclin D1 and c-Myc during transformation...
  26. ROLES OF PKC ISOFORMS IN COLONIC CARCINOGENESIS
    Marc Bissonnette; Fiscal Year: 2002
    ....
  27. BETA-CATENIN AND RAR INTERACT AT THE PKC ALPHA PROMOTER
    Frank Sinicrope; Fiscal Year: 2002
    ..We cloned the murine PKC alpha promoter, which contains a functional response element for retinoic acid receptor as well as two putative T-cell ..
  28. Investigating mechanisms of skeletal muscle degeneration in Myotonic Dystrophy
    James P Orengo; Fiscal Year: 2010
    ..A potential mechanism for increased CUGBP1 protein levels via phosphorylation through the PKC alpha pathway will also be examined...
  29. Effects of TPA on Leukemia and Solid Tumors
    Allan Conney; Fiscal Year: 2006
    ..or butyrate alone or as synergistic combinations are mediated through changes in the levels and translocation of PKC alpha, PKC beta and PKC delta, and on the expression of mRNAs for PKC alpha, PKC beta and PKC delta in TPA-sensitive ..
  30. PROTEOGLYCAN MEDIATED SIGNALING IN CELL ADHESION
    Anne Woods; Fiscal Year: 2006
    ..The goal is to identify new avenues for future interventional therapies to control adhesion phenotype that are more specific than at present. ..
  31. PKC as a Marker in Tamoxifen Resistant Brest Cancer
    Debra Tonetti; Fiscal Year: 2003
    ..We have previously reported that stable transfection of protein kinase C alpha (PKCa) into T47D human breast cancer cells results in a hormone-independent phenotype and TAM-resistant tumor growth...
  32. MOLECULAR ANALYSIS OF PLATELET INTEGRIN ACTIVATION
    James Chang; Fiscal Year: 2000
    ..Preliminary studies of stable HEL cell transfectants have demonstrated that heterologous expression of PKC alpha (which is absent in native HEL cells but abundant in platelets and therefore in terminally differentiated ..
  33. The 5-HT1A Receptor and Brain Development
    Probal Banerjee; Fiscal Year: 2005
    ..the mitogen activated protein kinase (MAPK) isozymes Erk1/2, which in turn, signal through protein kinase C alpha (PKCa) to cause inhibition of the proapoptotic protein caspase-3...
  34. Regulation of D-serine Neuromodulation by PKCalpha and PICK1
    ASIF MUSTAFA; Fiscal Year: 2009
    ..levels in vitro and from intact cells and monitor SR activity specifically with respect to phosphorylation by PKCa and interaction with PICK1...
  35. REGULATION AND DYNAMICS OF HEMIDESMOSOME DISASSEMBLY
    Isaac Rabinovitz; Fiscal Year: 2005
    ..Recent data suggest that integrin endocytosis is important for cell migration. We hypothesize PKC alpha regulates the disassembly of the hemidesmosome by regulating both the dissociation and internalization of the ..
  36. SUPPRESSION OF STEROIDOGENESIS BY PROTEIN KINASE C
    Mary Reyland; Fiscal Year: 2001
    ..We have previously shown that PKC alpha functions as a tonic suppressor of steroidogenesis by inhibiting expression of the steroid synthetic enzymes, ..
  37. REGULATION OF P GLYCOPROTEIN BY PHOSPHORYLATION
    Shakeel Ahmad; Fiscal Year: 1999
    ..The Third Aim will be to determine the effect of mutating the known or consensus PKC and PKA consensus phosphorylation sites in PGP on its ability to confer MDR to wild type or PKC-alpha-overexpressing MCF-7 cells. ..
  38. PEI Aerosol Delivery of ssDNA Expression Vector
    Yin Chen; Fiscal Year: 2004
    ..1. Production of PKCa targeting DNA enzyme in cells by ssDNA expression vector...
  39. MOLECULAR STUDIES OF GENE TARGETED OLIGONUCLEOTIDES
    CY STEIN; Fiscal Year: 2002
    ..Finally, in Specific Aim 3, we will amplify the delivery of alt-OMPs and chi-OMPs to cells by condensing them with a novel delivery agent, tetra (N-methylpyridyl) porphine. ..
  40. Detection of Metastatic Human Breast Cells by SECM
    SUSAN ROTENBERG; Fiscal Year: 2002
    ..The SECM will be harnessed to evaluate the role of protein kinase C alpha (pKC alpha) in the metastatic potential of normal and metastatic cultured human breast cells...
  41. C/EBP BETA IN HEPATOCYTE GENE EXPRESSION & PROLIFERATION
    Mario Chojkier; Fiscal Year: 2002
    ....
  42. EPA EFFECTS ON E-C COUPLING IN MESANGIAL CELLS
    Paul Schmitz; Fiscal Year: 2001
    ....
  43. Regulation of caveolae internalization
    JOHN ZEHMER; Fiscal Year: 2006
    ..Therefore, a deeper understanding of the regulation of caveolae budding could lead to clinical applications. [unreadable] [unreadable] [unreadable]..
  44. CELL SIGNAL TRANSDUCTION IN CORNEAL WOUND HEALING
    Haydee Bazan; Fiscal Year: 2006
    ..We will investigate the role of three specific protein kinase C (PKC) isoforms, PKCa, PKCe, and PKCu, as modulators of cell proliferation and migration and the relation between two MAPK cascades, ERK1/..
  45. METASTASIS SUPPRESSOR GENE IN HUMAN CUTANEOUS MELANOMA
    DANNY WELCH; Fiscal Year: 1999
    ..Third, they will identify critical KiSS-1 protein/macromolecule interactions using plaque library screening an immune co-precipitation experiments. ..
  46. TUMOR PROMOTION IN LLC PK1 EPITHELIAL CELLS
    Dan Rosson; Fiscal Year: 1999
    ..Preliminary data suggests that PKC alpha and delta isoforms are active in this process...
  47. PKC ISOZYMES AND INTESTINAL EPITHELIAL GROWTH CONTROL
    Jennifer Black; Fiscal Year: 2009
    ..PKC/PKC a signaling triggers a coordinated program of cell cycle withdrawal in intestinal epithelial cells, that PKC alpha activity regulates survival pathways in this system, that desensitization of PKC a signaling appears to be an ..
  48. CONFORMATIONAL PROBES & MOLECULAR PERCEPTION--PKC ALPHA
    J Ramer; Fiscal Year: 1999
    ..b>PKC alpha, a lipid and calcium dependent PKC isoform is important in normal cellular functions and as well as disease...
  49. PKC regulation of cell survival in intestinal cells
    MARYBETH PYSZ; Fiscal Year: 2007
    ..g., loss of PKC alpha and delta, increased levels of PKC iota) have been identified in colon carcinomas...
  50. ENERGY RESTRICTION, CELL SIGNALING AND CANCER PREVENTION
    Diane Birt; Fiscal Year: 2001
    ..tumor promotion by elevating glucocorticoid hormone (GCH) which reduces selected epidermal protein kinase C (PKC alpha and zeta) and blocks AP-1 induction...
  51. EXPRESSION OF PROTEIN KINASE C IN LENS EPITHELIAL CELLS
    Karen Gonzalez; Fiscal Year: 1999
    ..Enhancing the awareness of our students to continue graduate studies in Cellular and Molecular Biology will also be assessed. ..
  52. PKC Alpha as a Determinant of Ddrenergic Response
    Harvey Hahn; Fiscal Year: 2005
    ..Accomplishment of these aims will demonstrate that PKCalpha is a potentially important therapeutic target in ischemic heart disease and heart failure. ..
  53. Structure Function Studies Of Membrane Binding Proteins
    Wonhwa Cho; Fiscal Year: 2004
    ....
  54. Mast cell regulation by PKC and Akt
    Toshiaki Kawakami; Fiscal Year: 2005
    ..We also have preliminary data suggesting the role of C-terminal hydrophobic motif of PKCa in substrate recognition (Hypothesis 3)...
  55. Effects of Ang II and hypoxia on the blood-brain barrier
    MELISSA FLEEGAL DEMOTTA; Fiscal Year: 2006
    ..The goal is to investigate the effects of Ang II and hypoxia on PKC(alpha, beta-I, beta-II, gamma) expression and activity and its effects on tight junctions and BBB permeability. ..
  56. BONE FACTORS AND ENDOCHONDRAL OSSIFICATION
    Barbara Boyan; Fiscal Year: 2001
    ..It is suggested that these results will provide insight into the role these factors play in bone development and repair. ..
  57. Activation of the human neurtrophil
    HELEN KORCHAK; Fiscal Year: 2005
    ..V Establish the role of d-PKC in downregulation of signalling pathways triggered by the p60TNFR. Determine if d-PKC acts on a serine residue(s) in the juxtamembrane region or the death domain. ..
  58. PI and Wnt Signaling in Postmortem Brain of Biopolar and Schizophrenia Subjects
    Ghanshyam N Pandey; Fiscal Year: 2011
    ..This may eventually lead to i better understanding of the pathophysiology of BP or SZ illnesses and may aid in the development of more appropriate therapeutic agents for the treatment of these disorders. ..
  59. Influence of Sex and Gender on Musculoskeletal Health
    Barbara Boyan; Fiscal Year: 2004
    ..Worldwide current and future clinical trials will be discussed. Short papers on the workshop topics, future directions, and scientific updates will be published via Internet and summary papers in peer-reviewed journals. ..
  60. Molecular Mechanisms That Regulate CD11d Expression
    JOHN NOTI; Fiscal Year: 2004
    ..Transgenic analysis of the CD11d cis elements will be done to complement these studies. These studies will expand our understanding of normal myeloid development and leukemogenesis. ..
  61. ROLE OF MEMBRANES AND MINERALIZATION OF BONES AND TEETH
    Barbara Boyan; Fiscal Year: 2001
    ..This work will have major implications for our understanding of MV and how cells that calcify their matrix are able to regulate these extracellular organelles at distant sites. ..
  62. PKC Signaling in cAMP-Induced Pulmonary Vasodilation
    SCOTT BARMAN; Fiscal Year: 2010
    ..Progress of these studies will provide important knowledge towards the development of novel therapeutic agents that will help reduce the morbidity and mortality associated with pulmonary hypertension. ..
  63. PKC and Cellular Signaling in the Suicide Brain
    Ghanshyam Pandey; Fiscal Year: 2009
    ..This knowledge may aid in developing better therapeutic strategies for the treatment of suicidal behavior. ..