PIK3C3

Summary

Gene Symbol: PIK3C3
Description: phosphatidylinositol 3-kinase catalytic subunit type 3
Alias: VPS34, Vps34, hVps34, phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, phosphatidylinositol 3-kinase p100 subunit, phosphoinositide-3-kinase, class 3
Species: human

Top Publications

  1. pmc The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells
    Ping Gao
    Joint Laboratory of Apoptosis and Cancer Biology, The State Key Laboratory of Biomembrane and Membrane Biotechnology, Chinese Academy of Sciences, Beijing 100101, China
    J Biol Chem 285:25570-81. 2010
  2. ncbi HIV-1 gp120-induced TNF-{alpha} production by primary human macrophages is mediated by phosphatidylinositol-3 (PI-3) kinase and mitogen-activated protein (MAP) kinase pathways
    ChuHee Lee
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Leukoc Biol 78:1016-23. 2005
  3. ncbi Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells
    Naveed Ahmed Khan
    Division of Pediatric Infectious Diseases, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Neurovirol 9:584-93. 2003
  4. pmc Signaling mechanism of HIV-1 gp120 and virion-induced IL-1beta release in primary human macrophages
    Ricky Cheung
    Pulmonary, Allergy, and Critical Care, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 180:6675-84. 2008
  5. doi Akt/Nox2/NF-κB signaling pathway is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation
    Hong Sheng Zhang
    Beijing University of Technology, District of Chaoyang, China
    Arch Biochem Biophys 505:266-72. 2011
  6. ncbi HIV-1 Nef blocks transport of MHC class I molecules to the cell surface via a PI 3-kinase-dependent pathway
    S A Swann
    Departments of Medicine and Microbiology and Immunology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Virology 282:267-77. 2001
  7. ncbi Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells
    P Borgatti
    Institute of Human Anatomy, University of Ferrara, Italy
    Eur J Immunol 27:2805-11. 1997
  8. ncbi HIV-1 gp120-mediated apoptosis of T cells is regulated by the membrane tyrosine phosphatase CD45
    Appakkudal R Anand
    Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:12289-99. 2006
  9. pmc A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system
    S Volinia
    Ludwig Institute for Cancer Research, London, UK
    EMBO J 14:3339-48. 1995
  10. ncbi Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex
    C Panaretou
    Ludwig Institute of Cancer Research, University College London, Riding House Street, London W1P 8BT, UK
    J Biol Chem 272:2477-85. 1997

Research Grants

  1. Insulin Signaling and the Heart in Diabetes
    EVAN DALE ABEL; Fiscal Year: 2011
  2. Role of Endocytosis and Vesicular Trafficking in Podocyte Health and Filter Integ
    Puneet Garg; Fiscal Year: 2012
  3. Eric H Baehrecke; Fiscal Year: 2015
  4. Signaling by Vps34 in vertebrates
    Rachel S Salamon; Fiscal Year: 2012
  5. Cell Surface Receptors on T Cells and Macrophages
    CORNELIS P TERHORST; Fiscal Year: 2013
  6. RONALD K LIEM; Fiscal Year: 2016
  7. Henrik G Dohlman; Fiscal Year: 2014
  8. Tony Wyss-Coray; Fiscal Year: 2015
  9. Characterization of LC3-Associated Phagocytosis
    Jennifer Martinez; Fiscal Year: 2013
  10. Kun Liang Guan; Fiscal Year: 2016

Detail Information

Publications226 found, 100 shown here

  1. pmc The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells
    Ping Gao
    Joint Laboratory of Apoptosis and Cancer Biology, The State Key Laboratory of Biomembrane and Membrane Biotechnology, Chinese Academy of Sciences, Beijing 100101, China
    J Biol Chem 285:25570-81. 2010
    ..We then show that knockdown of Vps34 reduces gossypol-induced autophagy in both cell lines, and consistent with this, the phosphatidylinositol 3-..
  2. ncbi HIV-1 gp120-induced TNF-{alpha} production by primary human macrophages is mediated by phosphatidylinositol-3 (PI-3) kinase and mitogen-activated protein (MAP) kinase pathways
    ChuHee Lee
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Leukoc Biol 78:1016-23. 2005
    ..These gp120-triggered signaling pathways may be responsible for inappropriate production of proinflammatory cytokines by macrophages, which are believed to play a role in immunopathogenesis and in neurological sequelae of AIDS...
  3. ncbi Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells
    Naveed Ahmed Khan
    Division of Pediatric Infectious Diseases, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Neurovirol 9:584-93. 2003
    ..Further understanding and characterization of Tat-HBMEC interactions should help us understand HIV-1 neuropathogenesis and develop strategies to prevent HIV-1 encephalopathy...
  4. pmc Signaling mechanism of HIV-1 gp120 and virion-induced IL-1beta release in primary human macrophages
    Ricky Cheung
    Pulmonary, Allergy, and Critical Care, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 180:6675-84. 2008
    ..This study identifies a novel signaling mechanism for HIV-1-induced IL-1beta production by primary human macrophages that may be involved in the neuropathogenesis of HIV-associated dementia...
  5. doi Akt/Nox2/NF-κB signaling pathway is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation
    Hong Sheng Zhang
    Beijing University of Technology, District of Chaoyang, China
    Arch Biochem Biophys 505:266-72. 2011
    ....
  6. ncbi HIV-1 Nef blocks transport of MHC class I molecules to the cell surface via a PI 3-kinase-dependent pathway
    S A Swann
    Departments of Medicine and Microbiology and Immunology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Virology 282:267-77. 2001
    ..We propose that Nef diverts MHC-1 proteins into a PI 3-kinase-dependent transport pathway that prevents expression on the cell surface...
  7. ncbi Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells
    P Borgatti
    Institute of Human Anatomy, University of Ferrara, Italy
    Eur J Immunol 27:2805-11. 1997
    ..Taken together, these data suggest that the anti-apoptotic activity of low concentrations of Tat protein on Jurkat cells is mediated by a PI 3-kinase/Akt pathway...
  8. ncbi HIV-1 gp120-mediated apoptosis of T cells is regulated by the membrane tyrosine phosphatase CD45
    Appakkudal R Anand
    Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:12289-99. 2006
    ..These results suggest a novel CD45-mediated mechanism for the HIV envelope-induced apoptosis of T cells...
  9. pmc A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system
    S Volinia
    Ludwig Institute for Cancer Research, London, UK
    EMBO J 14:3339-48. 1995
    ..These results suggest that the major components of the yeast Vps intracellular trafficking complex are conserved in humans...
  10. ncbi Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex
    C Panaretou
    Ludwig Institute of Cancer Research, University College London, Riding House Street, London W1P 8BT, UK
    J Biol Chem 272:2477-85. 1997
    Genetic and biochemical studies have shown that the phosphatidylinositol (PtdIns) 3-kinase encoded by the yeast VPS34 gene is required for the efficient sorting and delivery of proteins to the vacuole...
  11. ncbi HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells
    Maria Chiara Deregibus
    Cattedra di Nefrologia, Dipartimento di Medicina Interna Università di Torino, Centro Ricerca Medicina Sperimentale CeRMS, Italy
    J Biol Chem 277:25195-202. 2002
    ....
  12. pmc Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade
    Isabelle Vergne
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 915 Camino de Salud, NE, Albuquerque, NM 87131, USA
    J Exp Med 198:653-9. 2003
    ..Ca2+ and calmodulin were required for a newly uncovered Ca2+/calmodulin phosphatidylinositol (PI)3 kinase hVPS34 cascade, essential for production of PI 3 phosphate (PI3P) on liposomes in vitro and on phagosomes in vivo...
  13. ncbi Human VPS34 and p150 are Rab7 interacting partners
    Mary Pat Stein
    Molecular Trafficking Laboratory, Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA
    Traffic 4:754-71. 2003
    ..Rab7 is required for late endosomal transport and here we establish that the phosphatidylinositol 3'-kinase hVPS34 and its adaptor protein p150 are rab7 interacting partners...
  14. ncbi Identification of PIK3C3 promoter variant associated with bipolar disorder and schizophrenia
    Pavla Stopkova
    Psychiatric Clinic, First Medical Faculty, Charles University, Prague, Czech Republic
    Biol Psychiatry 55:981-8. 2004
    ..One is PIK3C3, a member of the phosphatidylinositide 3-kinase family that maps closely to markers on 18q linked to both BD and ..
  15. ncbi hVps34 is a nutrient-regulated lipid kinase required for activation of p70 S6 kinase
    Maya P Byfield
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:33076-82. 2005
    ..We now present evidence that the Class III PI 3-kinase, hVps34, also regulates S6K1, and is a critical component of the nutrient sensing apparatus...
  16. pmc Amino acids mediate mTOR/raptor signaling through activation of class 3 phosphatidylinositol 3OH-kinase
    Takahiro Nobukuni
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Proc Natl Acad Sci U S A 102:14238-43. 2005
    ..amino acids control mTOR signaling is distinct from that of insulin and that, instead of signaling through components of the insulin/class 1 PI3K pathway, amino acids mediate mTOR activation by signaling through class 3 PI3K, hVps34.
  17. ncbi Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy
    Sophie Pattingre
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Cell 122:927-39. 2005
    ..This antiautophagy function of Bcl-2 may help maintain autophagy at levels that are compatible with cell survival, rather than cell death...
  18. ncbi The evolutionarily conserved domain of Beclin 1 is required for Vps34 binding, autophagy and tumor suppressor function
    Norihiko Furuya
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York, USA
    Autophagy 1:46-52. 2005
    ..function as a VPS and autophagy protein as part of a complex with Class III phosphatidylinositol 3'-kinase (PI3K)/Vps34. However, nothing is known about which domains of Atg6/Beclin 1 are required for its functional activity and ..
  19. ncbi Ambra1 regulates autophagy and development of the nervous system
    Gian Maria Fimia
    National Institute for Infectious Diseases, IRCCS L Spallanzani, 00149 Rome, Italy
    Nature 447:1121-5. 2007
    ....
  20. pmc Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 19:5360-72. 2008
    ..In yeast, two distinct PI3-kinase complexes are known: complex I (Vps34, Vps15, Vps30/Atg6, and Atg14) is involved in autophagy, and complex II (Vps34, Vps15, Vps30/Atg6, and Vps38) ..
  21. pmc Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase
    Qiming Sun
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:19211-6. 2008
    ....
  22. doi Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages
    Kohichi Matsunaga
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Nat Cell Biol 11:385-96. 2009
    Beclin 1, a protein essential for autophagy, binds to hVps34/Class III phosphatidylinositol-3-kinase and UVRAG. Here, we have identified two Beclin 1 associated proteins, Atg14L and Rubicon...
  23. pmc Human immunodeficiency virus type 1 Tat accelerates Kaposi sarcoma-associated herpesvirus Kaposin A-mediated tumorigenesis of transformed fibroblasts in vitro as well as in nude and immunocompetent mice
    Xiuying Chen
    Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, PR China
    Neoplasia 11:1272-84. 2009
    ....
  24. doi PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Cell Biol 12:362-71. 2010
    ..Depletion of the VPS34 or Beclin 1 subunits of PI(3)K-III causes cytokinesis arrest and an increased number of binucleate and ..
  25. doi A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic
    Sigrid B Thoresen
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, Oslo, Norway
    Exp Cell Res 316:3368-78. 2010
    ..with siRNA-mediated depletion of individual subunits indicated that a specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates both receptor degradation and cytokinesis, whereas ATG14L, a PI3K-III subunit ..
  26. pmc The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy
    Sabrina Di Bartolomeo
    Dulbecco Telethon Institute, University of Rome Tor Vergata, 00133 Rome, Italy
    J Cell Biol 191:155-68. 2010
    ..Autophagy initiation signaling requires both the ULK1 kinase and the BECLIN 1-VPS34 core complex to generate autophagosomes, double-membraned vesicles that transfer cellular contents to lysosomes...
  27. pmc The RUN domain of rubicon is important for hVps34 binding, lipid kinase inhibition, and autophagy suppression
    Qiming Sun
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    J Biol Chem 286:185-91. 2011
    ..Here, we demonstrate that Rubicon interacts with the PI3KC3 catalytic subunit hVps34 via its RUN domain...
  28. pmc RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly
    Brian O Bodemann
    Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390 9039, USA
    Cell 144:253-67. 2011
    ..Through direct binding to Exo84, RalB induces the assembly of catalytically active ULK1 and Beclin1-VPS34 complexes on the exocyst, which are required for isolation membrane formation and maturation...
  29. pmc Differential regulation of distinct Vps34 complexes by AMPK in nutrient stress and autophagy
    Joungmok Kim
    Department of Oral Biochemistry and Molecular Biology, Research Center for Tooth and Periodontal Tissue Regeneration, School of Dentistry, Kyung Hee University, Seoul 130 701, Korea
    Cell 152:290-303. 2013
    ..The class III phosphatidylinositol-3 kinase, Vps34, forms multiple complexes and regulates both intracellular vesicle trafficking and autophagy induction...
  30. pmc WASH inhibits autophagy through suppression of Beclin 1 ubiquitination
    Pengyan Xia
    Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    EMBO J 32:2685-96. 2013
    ..The lysine 437 ubiquitination of Beclin 1 enhances the association with Vps34 to promote Vps34 activity...
  31. pmc PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the beclin 1 complex
    Shanshan He
    1 Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, California 90033, USA 2
    Nat Cell Biol 15:1206-19. 2013
    ....
  32. doi Investigation of variants in the promoter region of PIK3C3 in schizophrenia
    Ruqi Tang
    Bio X Center, Shanghai Jiao Tong University, Shanghai, China
    Neurosci Lett 437:42-4. 2008
    The PIK3C3 gene has been implicated as a candidate gene for schizophrenia by functional evidence and genetic association studies. A series of previous studies have found susceptibility SNPs in promoter region...
  33. pmc hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells
    Ying Yan
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochem J 417:747-55. 2009
    The mammalian Class III PI3K (phosphoinositide 3-kinase), hVps34 [mammalian Vps (vacuolar protein sorting) 34 homologue], is an important regulator of vesicular trafficking, autophagy and nutrient sensing...
  34. ncbi HIV-1 Tat promotes monocyte chemoattractant protein-1 secretion followed by transmigration of monocytes
    I W Park
    Division of Experimental Medicine and Hematology Oncology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, MA 02115, USA
    Blood 97:352-8. 2001
    ..These data indicate that the HIV-1 Tat protein may act as a protocytokine by causing the release of MCP-1 from the endothelial monolayer, and thereby facilitating monocyte transmigration into tissues via a PKC signaling pathway...
  35. pmc Schizophrenia susceptibility genes directly implicated in the life cycles of pathogens: cytomegalovirus, influenza, herpes simplex, rubella, and Toxoplasma gondii
    C J Carter
    176 Downs Road, Hastings, East Sussex, TN34 2DZ, UK
    Schizophr Bull 35:1163-82. 2009
    ..bind to genes or proteins encoded by CALR, FEZ1, FYN, HSPA1B, IL2, HTR2A, KPNA3, MED12, MED15, MICB, NQO2, PAX6, PIK3C3, RANBP5, or TP53, while the cerebral infectivity of the herpes simplex virus is modified by Apolipoprotein E (APOE)..
  36. ncbi Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities
    J H Stack
    Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California, San Diego School of Medicine, La Jolla 92093 0668
    J Biol Chem 269:31552-62. 1994
    The Vps15 protein kinase and the Vps34 phosphatidylinositol 3-kinase have been shown to function as a membrane-associated complex which facilitates the delivery of proteins to the vacuole in yeast...
  37. doi Qualitative and quantitative characterization of autophagy in Caenorhabditis elegans by electron microscopy
    Timea Sigmond
    Laboratory of Developmental Genetics, Department of Genetics, Eotvos Lorand University, Budapest, Hungary
    Methods Enzymol 451:467-91. 2008
    ..Some autophagy-related gene mutants show characteristic ultrastuctural features, such as autophagosomes with membrane abnormalities (unc-51/Atg1) or the hypertrophy of multivesicular bodies (let-512/Vps34, bec-1/Atg6).
  38. doi Akt induces apoptosis in neuroblastoma cells expressing a C98X vasopressin mutant following autophagy suppression
    R Castino
    Laboratorio di Patologia Molecolare, Dipartimento di Scienze Mediche, Universita del Piemonte Orientale A Avogadro, Novara, Italy
    J Neuroendocrinol 20:1165-75. 2008
    ..This was shown by the expression of a Vps34 dominant negative, which down-regulates the PI3k class III-dependent signalling needed for autophagosome (APH) ..
  39. ncbi Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology
    K Takegawa
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093 0668, USA
    J Cell Sci 108:3745-56. 1995
    We have cloned the gene, vps34+, from the fission yeast Schizosaccharomyces pombe which encodes an 801 amino acid protein with phosphatidylinositol 3-kinase activity. The S...
  40. ncbi Inhibition of autophagy in mitotic animal cells
    Eeva Liisa Eskelinen
    Centre for High Resolution Imaging and Processing, MSI WTB complex, University of Dundee, School of Life Sciences, Dundee DD1 5EH, Scotland
    Traffic 3:878-93. 2002
    ..or in PDK1-/- embryonic stem cells, by microinjection of inhibitory antibodies against the class III PI3 kinase VPS34, or in cell lines lacking the p85 regulatory subunits of class IA PI3 kinases...
  41. pmc Roles of phosphatidylinositol 3-kinase in root hair growth
    Yuree Lee
    POSTECH UZH Global Research Laboratory, Division of Molecular Life Sciences, POSTECH, Pohang 790 784, Korea
    Plant Physiol 147:624-35. 2008
    ..elongation, we attempted to isolate Arabidopsis (Arabidopsis thaliana) mutant plants that did not express the gene VPS34 encoding the PI3K enzyme...
  42. pmc CD40 induces macrophage anti-Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes
    Rosa M Andrade
    Division of Infectious Diseases, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Clin Invest 116:2366-77. 2006
    ..Genetic and pharmacological approaches that impaired phosphoinositide-3-class 3 (PIK3C3), Rab7, vacuolar ATPase, and lysosomal enzymes revealed that vacuole/lysosome fusion mediated antimicrobial ..
  43. ncbi Myotubularin, a phosphatase deficient in myotubular myopathy, acts on phosphatidylinositol 3-kinase and phosphatidylinositol 3-phosphate pathway
    F Blondeau
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 1 rue Laurent Fries, BP 163, 67404 Illkirch Cedex, CU de Strasbourg, France
    Hum Mol Genet 9:2223-9. 2000
    ..phenotype similar to that of mutants of the vacuolar protein sorting (VPS) pathway and notably of mutants of VPS34, a phosphatidylinositol 3-kinase (PI3K). In S...
  44. doi Membrane phospholipid metabolism during phagocytosis in human neutrophils
    Reiko Minakami
    Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Genes Cells 15:409-24. 2010
    ..a putative participant in phagosome maturation, emerges at closed phagosomes as does the class III PtdIns 3-kinase Vps34. Although the small GTPases Rab5 and Rab7 are thought to contribute to phagosome maturation in macrophages, Rab5 ..
  45. pmc Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast
    Sangiliyandi Gurunathan
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    EMBO J 21:602-14. 2002
    ..e. VPS34 and VAM3, respectively) have no effect...
  46. pmc The receptor for advanced glycation end products (RAGE) sustains autophagy and limits apoptosis, promoting pancreatic tumor cell survival
    R Kang
    Department of Surgery, Hillman Cancer Center, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15232, USA
    Cell Death Differ 17:666-76. 2010
    ..is associated with decreased phosphorylation of mammalian target of rapamycin (mTOR) and increased Beclin-1/VPS34 autophagosome formation...
  47. pmc Aspergillus RabB Rab5 integrates acquisition of degradative identity with the long distance movement of early endosomes
    Juan F Abenza
    Departamento de Medicina Celular y Molecular, Centro de Investigaciones Biológicas del CSIC, Madrid 28040, Spain
    Mol Biol Cell 21:2756-69. 2010
    ..However, RabB-GTP is the sole recruiter to endosomes of Vps34 PI3K (phosphatidylinositol-3-kinase) and the phosphatidylinositol-3-phosphate [PI(3)P] effector AnVps19 and rabB ..
  48. ncbi Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
    P V Schu
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine
    Science 260:88-91. 1993
    The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae...
  49. ncbi Osmotic stress activates phosphatidylinositol-3,5-bisphosphate synthesis
    S K Dove
    Centre for Clinical Research in Immunology and Signalling, University of Birmingham, UK
    Nature 390:187-92. 1997
    ..This PtdIns(3,5)P2 accumulation only occurs in yeasts that have an active vps34-encoded PtdIns 3-OH kinase, showing that this latter kinase makes the PtdIns3P needed for PtdIns(3,5)P2 synthesis ..
  50. ncbi Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 126:191-203. 2006
    ..Our analysis reveals a requirement for both the catalytic (Vps34) and regulatory (Vps15) subunits of the sole phosphatidylinositol 3-kinase in yeast...
  51. pmc Rab5 modulates aggregation and toxicity of mutant huntingtin through macroautophagy in cell and fly models of Huntington disease
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 0XY, UK
    J Cell Sci 121:1649-60. 2008
    ..acts at an early stage of autophagosome formation in a macromolecular complex that contains beclin 1 (BECN1) and Vps34. Interestingly chemical or genetic inhibition of endocytosis also impeded macroautophagy, and enhanced aggregation ..
  52. pmc mVps34 is activated following high-resistance contractions
    Matthew G Mackenzie
    James Black Centre, University of Dundee, Dundee, DD1 5EH, UK
    J Physiol 587:253-60. 2009
    ..One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an ..
  53. pmc Structure and function of Vps15 in the endosomal G protein signaling pathway
    Erin J Heenan
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    Biochemistry 48:6390-401. 2009
    ..signaling at endosomes and requires two components of the sole phosphatidylinositol-3-kinase in yeast, Vps15 and Vps34. Vps15 contains multiple WD repeats and also binds to Gpa1 preferentially in the GDP-bound state; these ..
  54. ncbi Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
    M R Peterson
    Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    Curr Biol 9:159-62. 1999
    ..Vac1p/Pep7p/Vps19p, associates with Pep12p and binds phosphatidylinositol 3-phosphate (PI(3)P), the product of the Vps34 phosphatidylinositol 3-kinase (PI 3-kinase) [1] [2]...
  55. pmc The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting
    G G Tall
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235 9038, USA
    Mol Biol Cell 10:1873-89. 1999
    ..Vac1p FYVE finger mutant missorting phenotypes were suppressed by a defective allele of VPS34. These data indicate that PtdIns(3)P may perform a regulatory role, possibly involved in mediating Vac1p protein-..
  56. pmc Role of inositol trisphosphate receptors in autophagy in DT40 cells
    M Tariq Khan
    Department of Pathology and Cell Biology, Thomas Jefferson University School of Medicine, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 285:16912-20. 2010
    ..The amount of immunoprecipitated complexes of Bcl-2-Beclin-1 and Beclin-1-Vps34 were also not different between the two cell lines...
  57. ncbi Multiple genes and factors associated with bipolar disorder converge on growth factor and stress activated kinase pathways controlling translation initiation: implications for oligodendrocyte viability
    C J Carter
    Neurochem Int 50:461-90. 2007
    ..Several form most of the components of a phosphatidyl-inositol signalling/AKT1 survival pathway (PIK3C3, PIP5K2A, PLCG1, SYNJ1, IMPA2, AKT1, GSK3B, TCF4) which is activated by growth factors (BDNF, NRG1) and also by ..
  58. pmc SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages
    Scott B Berger
    Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Nat Immunol 11:920-7. 2010
    ..SLAM recruited a complex containing the intracellular class III phosphatidylinositol kinase Vps34, its regulatory protein kinase Vps15 and the autophagy-associated molecule beclin-1 to the phagosome, which was ..
  59. pmc Lipid binding requirements for oxysterol-binding protein Kes1 inhibition of autophagy and endosome-trans-Golgi trafficking pathways
    Marissa A LeBlanc
    Department of Pediatrics and Biochemistry and Molecular Biology, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Biol Chem 285:33875-84. 2010
    ..regulation of the autophagy/cytoplasm to the vacuole trafficking pathway was prevented by increasing expression of the PI3K Vps34, suggesting that it is the Kes1-mediated decrease in PI3P level that contributes to this regulation.
  60. pmc Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy
    Andrew E Wurmser
    Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California at San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA
    J Cell Biol 158:761-72. 2002
    ..Current evidence suggests that the Vps34 phosphatidylinositol 3-kinase is essential for macroautophagy, a starvation-induced autophagy pathway (Kihara et ..
  61. ncbi The phosphatidylinositol 3-kinase Vps34p of the human pathogenic yeast Candida albicans is a multifunctional protein that interacts with the putative vacuolar H+ -ATPase subunit Vma7p
    Raimund Eck
    Department of Infection Biology, Hans Knöll Institute for Natural Products Research, Beutenbergstrasse 11, D 07745 Jena, Germany
    Int J Med Microbiol 295:57-66. 2005
    ..In addition, the vps34 null mutant of C. albicans shows defective autophagocytosis. The relevance of these functions for virulence of C...
  62. pmc The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila
    Gabor Juhasz
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    J Cell Biol 181:655-66. 2008
    Degradation of cytoplasmic components by autophagy requires the class III phosphatidylinositol 3 (PI(3))-kinase Vps34, but the mechanisms by which this kinase and its lipid product PI(3) phosphate (PI(3)P) promote autophagy are unclear...
  63. pmc Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway
    Xiang Zhou
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:9424-9. 2010
    The lipid kinase PIK3C3 (also called Vps34) regulates both the endosomal and autophagic pathways. However, the effect of inactivating PIK3C3 on neuronal endosomal versus autophagic processes in vivo has not been studied...
  64. pmc Membrane transport in Caenorhabditis elegans: an essential role for VPS34 at the nuclear membrane
    Lorenz Roggo
    Department of Biology, University of Fribourg, CH 1700 Fribourg, Switzerland
    EMBO J 21:1673-83. 2002
    Here we present a detailed genetic analysis of let-512/vps34 that encodes the Caenorhabditis elegans homologue of the yeast phosphatidylinositol 3-kinase Vps34p...
  65. pmc Shaping development of autophagy inhibitors with the structure of the lipid kinase Vps34
    Simon Miller
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Science 327:1638-42. 2010
    Phosphoinositide 3-kinases (PI3Ks) are lipid kinases with diverse roles in health and disease. The primordial PI3K, Vps34, is present in all eukaryotes and has essential roles in autophagy, membrane trafficking, and cell signaling...
  66. pmc Negative regulation of Vps34 by Cdk mediated phosphorylation
    Tsuyoshi Furuya
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 38:500-11. 2010
    Vacuolar protein sorting 34 (Vps34) complexes, the class III PtdIns3 kinase, specifically phosphorylate the D3 position of PtdIns to produce PtdIns3P...
  67. doi Inhibition of PI3k class III-dependent autophagy prevents apoptosis and necrosis by oxidative stress in dopaminergic neuroblastoma cells
    Roberta Castino
    Laboratorio di Patologia Molecolare, Dipartimento di Scienze Mediche, Universita del Piemonte Orientale A Avogadro, Novara, Italy
    Toxicol Sci 117:152-62. 2010
    ..SH-SY5Y cells, 200microM H(2)O(2) rapidly induced the formation of LC3-positive autophagic vacuoles and of beclin1-Vps34 double-positive macroaggregates...
  68. pmc Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast
    J H Stack
    Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California, San Diego School of Medicine, La Jolla 92093 0668, USA
    J Cell Biol 129:321-34. 1995
    A membrane-associated complex composed of the Vps15 protein kinase and the Vps34 phosphatidylinositol 3-kinase (PtdIns 3-kinase) is essential for the delivery of proteins to the yeast vacuole...
  69. ncbi The HIV-1 nef protein interferes with phosphatidylinositol 3-kinase activation 1
    A Graziani
    Institute for Cancer Research and Treatment IRCC, University of Torino Medical School, 10126 Torino, Italy
    J Biol Chem 271:6590-3. 1996
    ..These data show that Nef affects selectively the PI 3-kinase signaling pathway and suggest that this interference results in some of the HIV adverse effects on host cell functions...
  70. ncbi Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains
    C G Burd
    Howard Hughes Medical Institute, University of California, San Diego School of Medicine, La Jolla 92093 0668, USA
    Mol Cell 2:157-62. 1998
    ..Using an in vivo assay in yeast that detects Vps34 PI(3)K-dependent intracellular localization of a GFP reporter protein, and in vitro lipid-binding assays, we ..
  71. pmc An enzymatic cascade of Rab5 effectors regulates phosphoinositide turnover in the endocytic pathway
    Hye Won Shin
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany
    J Cell Biol 170:607-18. 2005
    ..The small GTPase Rab5 interacts with two PI 3-kinases, Vps34 and PI3Kbeta, suggesting that it regulates the production of 3-PIs at various stages of the early endocytic ..
  72. doi Autophagy by hepatitis B virus and for hepatitis B virus
    Donna Sir
    Department of Molecular Microbiology and Immunology lt University of Southern California lt Los Angeles, CA, USA
    Autophagy 6:548-9. 2010
    ..The persistent activation of autophagy in hepatocytes by HBV during chronic infection may play an important role in HBV pathogenesis...
  73. pmc Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor
    Simon Miller
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Autophagy 6:805-7. 2010
    b>Vps34 is the ancestral phosphatidylinositol 3-kinase (PtdIns3K) isoform and is essential for endosomal trafficking of proteins to the vacuole/lysosome, autophagy and phagocytosis...
  74. pmc Discovery of potent and selective covalent inhibitors of JNK
    Tinghu Zhang
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Chem Biol 19:140-54. 2012
    ..Potential lead compounds have also been identified for kinases, including IRAK1, PIK3C3, PIP4K2C, and PIP5K3.
  75. pmc The autophagy regulator Rubicon is a feedback inhibitor of CARD9-mediated host innate immunity
    Chul Su Yang
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
    Cell Host Microbe 11:277-89. 2012
    ..Beclin-1-interacting cysteine-rich-containing Rubicon protein associates constitutively with the Beclin-UVRAG-Vps34 complex under normal conditions to regulate autophagy...
  76. pmc DEDD, a novel tumor repressor, reverses epithelial-mesenchymal transition by activating selective autophagy
    Qi Lv
    Molecular Immunology and Pharmacology Group, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Autophagy 8:1675-6. 2012
    ..We found that DEDD physically interacts with the class III PtdIns 3-kinase complex containing PIK3C3 and BECN1, which controls critical aspects of autophagy; this interaction activates autophagy and induces the ..
  77. pmc Deletion of autophagy-related 5 (Atg5) and Pik3c3 genes in the lens causes cataract independent of programmed organelle degradation
    Hideaki Morishita
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    J Biol Chem 288:11436-47. 2013
    ..We also produced lens-specific Pik3c3 knock-out mice to elucidate the possible involvement of Atg5-independent alternative autophagy, which is proposed ..
  78. doi mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6
    Francesca Nazio
    Dulbecco Telethon Institute at the Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy
    Nat Cell Biol 15:406-16. 2013
    ..mTOR (mammalian target of rapamycin) inhibition, autophagosome formation is primed by the ULK1 and the beclin-1-Vps34-AMBRA1 complexes, which are linked together by a scaffold platform, the exocyst...
  79. doi Class III PI3K Vps34: essential roles in autophagy, endocytosis, and heart and liver function
    Nadia Jaber
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794, USA
    Ann N Y Acad Sci 1280:48-51. 2013
    ..One family member, Vps34, is of particular interest because it is the only PI3-kinase identified in yeast and it has been evolutionarily ..
  80. pmc Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease
    Ivan Nemazanyy
    INSERM, U845, Paris, France
    EMBO Mol Med 5:870-90. 2013
    The complex of Vacuolar Protein Sorting 34 and 15 (Vps34 and Vps15) has Class III phosphatidylinositol 3-kinase activity and putative roles in nutrient sensing, mammalian Target Of Rapamycin (mTOR) activation by amino acids, cell growth, ..
  81. doi Nuclear trafficking of EGFR by Vps34 represses Arf expression to promote lung tumor cell survival
    D Daydé
    Equipe Bases Moléculaires de la Progression des Cancers du Poumon, Centre de Recherche INSERM U823, Grenoble, France
    Oncogene 35:3986-94. 2016
    ..We unraveled the vesicular trafficking protein Vps34 as a mediator of EGFR nuclear trafficking and showed that its neutralization prevents the accumulation of EGFR to ..
  82. pmc PtdIns(4,5)P2 signaling regulates ATG14 and autophagy
    Xiaojun Tan
    Program in Molecular and Cellular Pharmacology, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53706
    Proc Natl Acad Sci U S A 113:10896-901. 2016
    ..The Barkor/ATG14(L)-VPS34 (a class III phosphatidylinositol 3-kinase) complex and its product phosphatidylinositol 3-phosphate [PtdIns(3)P] ..
  83. ncbi HIV-1 Nef plays an essential role in two independent processes in CD4 down-regulation: dissociation of the CD4-p56(lck) complex and targeting of CD4 to lysosomes
    Y H Kim
    Laboratory of Molecular Virology, Samsung Biomedical Research Institute, Seoul, Korea
    Virology 257:208-19. 1999
    ....
  84. ncbi Direct involvement of phosphatidylinositol 4-phosphate in secretion in the yeast Saccharomyces cerevisiae
    H Hama
    Department of Biology, Utah State University, Logan, Utah 84322 5305, USA
    J Biol Chem 274:34294-300. 1999
    ..This effect is specific because overexpression of the other PtdIns 4-kinase gene (STT4) or a PtdIns 3-kinase gene (VPS34) did not rescue sec14-3 cells...
  85. ncbi HIV-1 Nef downregulates MHC-I by a PACS-1- and PI3K-regulated ARF6 endocytic pathway
    Anastassia D Blagoveshchenskaya
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Cell 111:853-66. 2002
    ..These data provide new insights into the cellular basis of HIV-1 immunoevasion...
  86. ncbi TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation
    Belinda S Hall
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
    J Biol Chem 281:27600-12. 2006
    ..The yeast class III phosphatidylinositol 3-kinase Vps34p, and its human orthologue hVPS34, are implicated in control of several key pathways, including endosome to lysosome transport, retrograde endosome ..
  87. pmc FADD and caspase-8 control the outcome of autophagic signaling in proliferating T cells
    Bryan D Bell
    Department of Molecular Biology and Biochemistry and Center for Immunology, University of California, Irvine, Irvine, CA 92697 3900, USA
    Proc Natl Acad Sci U S A 105:16677-82. 2008
    ..Inhibition of autophagic signaling with 3-methyladenine, dominant-negative Vps34, or Atg7 shRNA rescued T cells expressing a dominant-negative FADD protein...
  88. pmc The Beclin 1-VPS34 complex--at the crossroads of autophagy and beyond
    Sarah F Funderburk
    Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Trends Cell Biol 20:355-62. 2010
    ..Beclin 1 performs these roles as part of a core complex that contains vacuolar sorting protein 34 (VPS34), a class III phosphatidylinositol-3 kinase...
  89. pmc Erythropoietin plus insulin-like growth factor-I protects against neuronal damage in a murine model of human immunodeficiency virus-associated neurocognitive disorders
    Yeon Joo Kang
    Del E Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford Burnham Medical Research Institute, La Jolla, CA, USA
    Ann Neurol 68:342-52. 2010
    ..Here, we report that chronic treatment with erythropoietin (EPO) and insulin-like growth factor-I (IGF-I) protects against HIV/gp120-mediated neuronal damage in culture and in vivo...
  90. pmc A novel mass assay to quantify the bioactive lipid PtdIns3P in various biological samples
    Gaëtan Chicanne
    INSERM, U1048 and Université Toulouse 3, I2MC, Avenue Jean Poulhès BP84225, 31432 Toulouse cedex 04, France
    Biochem J 447:17-23. 2012
    ..Using this assay, we show an increase in the mass level of PtdIns3P in mouse and human platelets following stimulation, loss of this lipid in Vps34-deficient yeasts and its relative enrichment in early endosomes isolated from BHK cells.
  91. pmc Sunitinib-induced morpho-functional changes and drug effectiveness in malignant solitary fibrous tumours
    Rosalin D Spagnuolo
    Laboratory of Experimental Molecular Pathology, Department of Diagnostic Pathology and Laboratory, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy
    Oncotarget 7:45015-45026. 2016
    ..Finally, we also found that PDGFRB is one of the components of a complex that includes Beclin 1 and VPS34.The results of these tissue-based analyses provide new insights into sunitinib's mechanism of action in SFT ..
  92. pmc Dynamics and architecture of the NRBF2-containing phosphatidylinositol 3-kinase complex I of autophagy
    Lindsey N Young
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720
    Proc Natl Acad Sci U S A 113:8224-9. 2016
    ..NRBF2 enhances the lipid kinase activity of the catalytic subunit, VPS34, by roughly 10-fold...
  93. pmc Angiogenic Factor AGGF1 Activates Autophagy with an Essential Role in Therapeutic Angiogenesis for Heart Disease
    Qiulun Lu
    Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, P R China
    PLoS Biol 14:e1002529. 2016
    ..Mechanistically, AGGF1 initiates autophagy by activating JNK, which leads to activation of Vps34 lipid kinase and the assembly of Becn1-Vps34-Atg14 complex involved in the initiation of autophagy...
  94. pmc Vps34 regulates Rab7 and late endocytic trafficking through recruitment of the GTPase-activating protein Armus
    Nadia Jaber
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook NY11794, USA
    J Cell Sci 129:4424-4435. 2016
    The class III phosphoinositide 3-kinase (PI3K) Vps34 (also known as PIK3C3 in mammals) produces phosphatidylinositol 3-phosphate [PI(3)P] on both early and late endosome membranes to control membrane dynamics...
  95. ncbi Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function
    Daniele E Ejzykowicz
    Department of Biological Sciences, California State University Long Beach, 1250 Bellflower Blvd, Long Beach, CA, 90840, USA
    Curr Genet . 2016
    ..inhibition and establish a super HHY (s-HHY) subgroup of genes comprised of ARF1, CHC1, DRS2, SAC1, VPS1, VPS34, VPS45, VPS52, and VPS54 that function exclusively or inclusively at trans-Golgi and late endosome interface...
  96. pmc A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development
    K Zhou
    Department of Biology, Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0634, USA
    Mol Cell Biol 15:5645-56. 1995
    ..Antisense expression of DdPIK5 (a putative homolog of the Saccharomyces cerevisiae VPS34) led to a defect in the growth of D. discoideum cells on bacterial lawns and abnormal development...
  97. ncbi Characterization of staurosporine-sensitive mutants of Saccharomyces cerevisiae: vacuolar functions affect staurosporine sensitivity
    S Yoshida
    Central Laboratories for Key Technology, Kirin Brewery Co Ltd, Japan
    Mol Gen Genet 263:877-88. 2000
    ..integration mapping showed that the genes STT2/ STT6, STT5, STT7, STT8 and STT9 are allelic to VPS18, ERG10, GPI1, VPS34 and VPS11, respectively...
  98. pmc Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells
    D J Gillooly
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    EMBO J 19:4577-88. 2000
    ..While yeast cells deficient in PI3K activity (vps15 and vps34 mutants) were not labelled, PI(3)P was found on intralumenal vesicles of endosomes and vacuoles of wild-type yeast...
  99. pmc Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae
    A Kihara
    Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444 8585, Japan
    J Cell Biol 152:519-30. 2001
    ..These results indicate that Vps30p functions as a subunit of a Vps34 PtdIns 3-kinase complex(es)...
  100. ncbi Assignment of the phosphoinositide-3-kinase, class 3 (PIK3C3) gene to porcine chromosome 6q22-->q23 by somatic cell and radiation hybrid panel mapping
    J H Kim
    Division of Applied Life Science, Gyeongsang National University, Jinju, Korea
    Cytogenet Genome Res 108:362. 2005
  101. ncbi Phosphatidylinositol 3-kinase VPS34 of Candida albicans is involved in filamentous growth, secretion of aspartic proteases, and intracellular detoxification
    Ana Kitanovic
    Clinic for Internal Medicine II, Molecular Biology Laboratory, Friedrich Schiller University Jena, Erlanger Allee 101, D 07747 Jena, Germany
    FEMS Yeast Res 5:431-9. 2005
    ..albicans vps34 null mutant strain...

Research Grants29

  1. Insulin Signaling and the Heart in Diabetes
    EVAN DALE ABEL; Fiscal Year: 2011
    ..activation of Akt will increase the association of Beclin1 with Bcl2, thereby inhibiting the activity of the Vps34 complex that promotes autophagic vesicle enucleation...
  2. Role of Endocytosis and Vesicular Trafficking in Podocyte Health and Filter Integ
    Puneet Garg; Fiscal Year: 2012
    ..b>Vps34 (PI3K class III) is member of the phosphoinositides 3-kinsae (PI3K) family of lipid kinases and is important in ..
  3. Eric H Baehrecke; Fiscal Year: 2015
    ..Beclin1 (Atg6 in flies) is a core component of the evolutionarily conserved Vps34/class III phosphatidylinositol 3 (PI3) kinase complex that regulates the formation of PI3 phosphate (PI3P) lipids...
  4. Signaling by Vps34 in vertebrates
    Rachel S Salamon; Fiscal Year: 2012
    ..The mammalian Class III PI 3-kinase, hVps34, is required for both vesicular trafficking and autophagy...
  5. Cell Surface Receptors on T Cells and Macrophages
    CORNELIS P TERHORST; Fiscal Year: 2013
    ..typhimurium. In the lipid bilayer of the phagosome Slamf1 recruits the intracellular Class III PI-3'kinase Vps34/15 together with several proteins that partake in autophagy to its cytoplasmic tail...
  6. RONALD K LIEM; Fiscal Year: 2016
    ..PI3P is a phosphoinositide primarily synthesized by lipid kinase Vps34 and acts as a master regulator of the endosomal and autophagy pathways...
  7. Henrik G Dohlman; Fiscal Year: 2014
    ..4-kinases (Pik1 &Stt4) and two subunits of the phosphatidylinositol 3-kinase complex (Vps15 &Vps34). We postulate that these enzymes promote the selective activation of scaffolded MAP kinases...
  8. Tony Wyss-Coray; Fiscal Year: 2015
    ..These studies show that full length beclin 1 together with its binding partner vps34 is strongly reduced in AD brains and that reducing beclin 1 in AD model mice results in more A[unreadable] ..
  9. Characterization of LC3-Associated Phagocytosis
    Jennifer Martinez; Fiscal Year: 2013
    ..In short, ATG proteins, with Beclin-1 and the class III PI3K, VPS34, initiate the formation of the isolation membrane or autophagophore...
  10. Kun Liang Guan; Fiscal Year: 2016
    ..of AMPK in regulating ULK1 activation and function in autophagy 3 To investigate the mechanism of AMPK in VPS34 regulation during autophagy PUBLIC HEALTH RELEVANCE: Autophagy is a regulated catabolic process in which the cell ..
  11. Wei Xing Zong; Fiscal Year: 2015
    ..Class III PI3K is composed of a sole member, Vps34, that converts PtdIns to PI(3)P...
  12. Zhenyu Yue; Fiscal Year: 2016
    ..We have a long-standing interest in understanding Beclin1/Vps34 (class III PtdIns-3-kinase!, which is a key regulator of autophagy...
  13. Cross-regulation of apoptosis and autophagy as a molecular basis for reversal of
    Hannah Rabinowich; Fiscal Year: 2012
    ..hypothesize that essential components of the autophagosome formation process, particularly Beclin-1/Atg6, UVRAG, Vps34 and Atg7, are involved in a constant crosstalk between autophagy and apoptosis through their dual function of ..
  14. Nadia Jaber; Fiscal Year: 2015
    DESCRIPTION (provided by applicant): Vps34 is a lipid kinase that regulates membrane trafficking pathways like endocytosis and autophagy by generating phosphoinositol 3-phosphate, a lipid signaling molecule...
  15. Jae U Jung; Fiscal Year: 2015
    ..While Rubicon primarily associates with the Beclin1-Vps34 autophagy complex and suppresses autophagosome maturation step under normal conditions, it is rapidly recruited to ..
  16. Endosome sorting of human beta2-adrenoceptors
    Robert Moore; Fiscal Year: 2009
    ..elucidate the role of the human Vps34-interacting protein, the rab7 GPTase, in the regulation of P2AR recycling;(3)...
  17. ANALYSIS OF BECLIN 1 IN AUTOPHAGY AND TUMOR SUPPRESSION
    Beth Levine; Fiscal Year: 2009
    ..human MCF7 breast carcinoma cell model to determine whether the Beclin 1-binding partner, Class III PI3-K/hVps34, (involved in autophagic vesicle nucleation) and whether autophagy genes involved in a later stage of autophagy (..
  18. Regulation and Function of hVps34 in Insulin Signaling
    Jonathan M Backer; Fiscal Year: 2010
    ..the function of p85/p110 PI 3-kinases in insulin responsive cells is well documented, the Class III PI 3-kinase, hVps34, also plays an significant role in insulin action...
  19. Training in proteomics of novel kinase substrates for neurodegeneration
    Charleen Chu; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  20. LRRK2 Proteomics: Kinase/GTPase Hypothesis and Interaction Partners
    Zhenyu Yue; Fiscal Year: 2007
    ..It will also reveal roles of LRRK2 in regulating or interacting with other molecular pathways. This proposal will provide valuable information for PD therapeutic design directed towards enzymatic activity of LRRK2. [unreadable]..
  21. Relationship of Apo B and BHMT: Nutrition and Physiology
    Janet Sparks; Fiscal Year: 2004
    ..A potential link of two pathways whose products or intermediates are associated with increased cardiovascular disease risk could be established. ..
  22. Hepatic Steatosis Modulation by Apo B Gene Transcription
    JANET DEHOFF SPARKS; Fiscal Year: 2010
    ....
  23. THE PARKINSONIAN 6 HYDROXYDOPAMINE MODEL
    Charleen Chu; Fiscal Year: 2004
    ....
  24. Autophagy: A Novel Antiviral Host Defense Pathway
    Beth Levine; Fiscal Year: 2007
    ..Together, these studies will provide novel insights about the role of autophagy in antiviral host defense and the role of viral evasion of autophagy in neurovirulence. ..
  25. Regulation of PTEN-induced kinase 1 (PINK 1)
    Charleen Chu; Fiscal Year: 2007
    ..In addition, a group of immunochemical and molecular reagents will have been created that can facilitate the work of other investigators studying mechanisms of Parkinson's and related diseases. [unreadable] [unreadable]..
  26. BCL2 AND BECLIN AND ALPHAVIRUS PATHOGENESIS
    Beth Levine; Fiscal Year: 2003
    ..The proposed studies will provide novel insights into host cellular mechanisms that regulate whether neurons live or die in responses to viral infection. ..
  27. Identifying & Characterizing Prodromal Schizophrenia
    Todd Lencz; Fiscal Year: 2007
    ..This study will provide the candidate with the knowledge and research experience necessary to apply for an R01 to expand upon this research from a neurodevelopmental perspective. ..
  28. Identifying Molecular Subtypes of Schizophrenia: A Novel Genomic Approach
    Todd Lencz; Fiscal Year: 2010
    ..Findings will create new opportunities for diagnosis and prediction of schizophrenia, and for understanding its biology. ..