perforin

Summary

Gene Symbol: perforin
Description: perforin 1
Alias: FLH2, HPLH2, PFN1, PFP, perforin-1, cytolysin, lymphocyte pore forming protein, perforin 1 (pore forming protein)
Species: human

Top Publications

  1. pmc Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products
    K Yamamoto
    Department of Bacteriology and Serology, Osaka University, Japan
    Infect Immun 58:4106-16. 1990
  2. ncbi [On-line sequential hemodiafiltration (HDF-OL-S): a new therapeutic option]
    J Kanter
    Servicio de Nefrologia, Unidad de Hemodiálisis, Hospital G U Gregorio Marañón, Madrid, Espana
    Nefrologia 28:433-8. 2008
  3. pmc Cytolysins augment superantigen penetration of stratified mucosa
    Amanda J Brosnahan
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 182:2364-73. 2009
  4. doi Characterization of pertussis toxoid by two-dimensional liquid chromatography-tandem mass spectrometry
    Manorama Tummala
    Baxter Healthcare, Technology Resources, Round Lake, IL 60073, USA
    Anal Biochem 401:295-302. 2010
  5. pmc Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture after myocardial infarction
    Jens Fielitz
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 104:4377-82. 2007
  6. ncbi Structure and function of human perforin
    M G Lichtenheld
    Department of Microbiology and Immunology, University of Miami, School of Medicine, Florida 33101
    Nature 335:448-51. 1988
  7. ncbi Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules
    C Andrin
    Molecular Biology of Membranes Research Group, University of Alberta, Edmonton, Canada
    Biochemistry 37:10386-94. 1998
  8. ncbi Perforin gene defects in familial hemophagocytic lymphohistiocytosis
    S E Stepp
    Department of Pathology and the Graduate Program in Immunology, University of Texas Southwestern Medical School, Dallas, TX 75235, USA
    Science 286:1957-9. 1999
  9. ncbi Cytotoxic cell granule-mediated apoptosis: perforin delivers granzyme B-serglycin complexes into target cells without plasma membrane pore formation
    Sunil S Metkar
    Evanston Northwestern Healthcare Research Institute, Evanston, IL 60201, USA
    Immunity 16:417-28. 2002
  10. ncbi HIV-specific CD8+ T cell proliferation is coupled to perforin expression and is maintained in nonprogressors
    Stephen A Migueles
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Nat Immunol 3:1061-8. 2002

Research Grants

  1. Epidemiology of Patellofemoral Pain Syndrome: Identifying Gender Specific Risk Fa
    MICHELLE CLARA BOLING; Fiscal Year: 2011
  2. Mechanisms of host defense against membrane damage by pore-forming toxins
    MARY O'RIORDAN; Fiscal Year: 2013
  3. Mechanism of Cell Membrane Targeting by Vibrio Cholerae Cytolysin
    RICHARD A OLSON; Fiscal Year: 2012
  4. Prevention of Acute Lung Injury by IL-6 activated STAT3
    Ana Moran; Fiscal Year: 2010
  5. NANCY ELIZABETH FREITAG; Fiscal Year: 2016
  6. Post-translation regulation of Listeria monocytogenes virulence factors
    NANCY ELIZABETH FREITAG; Fiscal Year: 2010
  7. Michael G Caparon; Fiscal Year: 2014
  8. SpyA, a novel S. pyogenes ADP-ribosyltransferase
    STEPHEN L MOSELEY; Fiscal Year: 2010
  9. ADAM JONATHAN RATNER; Fiscal Year: 2015
  10. MATTHEW EUGENE PIPKIN; Fiscal Year: 2015

Detail Information

Publications243 found, 100 shown here

  1. pmc Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products
    K Yamamoto
    Department of Bacteriology and Serology, Osaka University, Japan
    Infect Immun 58:4106-16. 1990
    Vibrio cholerae O1 biotype El Tor produces and secretes a 65-kDa cytolysin/hemolysin into the culture medium. We cloned the structural gene (hlyA) for the cytolysin from the total DNA of a V. cholerae O1 El Tor strain, N86...
  2. ncbi [On-line sequential hemodiafiltration (HDF-OL-S): a new therapeutic option]
    J Kanter
    Servicio de Nefrologia, Unidad de Hemodiálisis, Hospital G U Gregorio Marañón, Madrid, Espana
    Nefrologia 28:433-8. 2008
    ..OL-S-HDF initially begins in postdilution mode changing to predilution once the transmembrane pressure (TMP) reached 250 mmHg...
  3. pmc Cytolysins augment superantigen penetration of stratified mucosa
    Amanda J Brosnahan
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 182:2364-73. 2009
    ..The staphylococcal cytolysin alpha-toxin and the streptococcal cytolysin streptolysin O enhanced penetration of toxic shock syndrome toxin-1 ..
  4. doi Characterization of pertussis toxoid by two-dimensional liquid chromatography-tandem mass spectrometry
    Manorama Tummala
    Baxter Healthcare, Technology Resources, Round Lake, IL 60073, USA
    Anal Biochem 401:295-302. 2010
    ..An analytical-scale high-performance liquid chromatography (HPLC) instrument using a pentafluorophenyl (PFP) column was used as the first-dimensional LC for fraction collection...
  5. pmc Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture after myocardial infarction
    Jens Fielitz
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 104:4377-82. 2007
    ..These findings reveal an important role of MuRF3 in maintaining cardiac integrity and function after acute myocardial infarction and suggest that turnover of FHL2 and gamma-filamin contributes to this cardioprotective function of MuRF3...
  6. ncbi Structure and function of human perforin
    M G Lichtenheld
    Department of Microbiology and Immunology, University of Miami, School of Medicine, Florida 33101
    Nature 335:448-51. 1988
    b>Perforin (P1) is a cytolytic protein with similarity to complement component C9...
  7. ncbi Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules
    C Andrin
    Molecular Biology of Membranes Research Group, University of Alberta, Edmonton, Canada
    Biochemistry 37:10386-94. 1998
    ..In perforin knock-out mice calreticulin is still targeted into the granules...
  8. ncbi Perforin gene defects in familial hemophagocytic lymphohistiocytosis
    S E Stepp
    Department of Pathology and the Graduate Program in Immunology, University of Texas Southwestern Medical School, Dallas, TX 75235, USA
    Science 286:1957-9. 1999
    ..3-22, 10q21-22, or another as yet undefined locus. Sequencing of the coding regions of the perforin gene of eight unrelated 10q21-22-linked FHL patients revealed homozygous nonsense mutations in four patients and ..
  9. ncbi Cytotoxic cell granule-mediated apoptosis: perforin delivers granzyme B-serglycin complexes into target cells without plasma membrane pore formation
    Sunil S Metkar
    Evanston Northwestern Healthcare Research Institute, Evanston, IL 60201, USA
    Immunity 16:417-28. 2002
    The mechanism underlying perforin (PFN)-dependent delivery of apoptotic granzymes during cytotoxic cell granule-mediated death remains speculative...
  10. ncbi HIV-specific CD8+ T cell proliferation is coupled to perforin expression and is maintained in nonprogressors
    Stephen A Migueles
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Nat Immunol 3:1061-8. 2002
    ..This proliferation was coupled to increases in perforin expression...
  11. ncbi Serial peripheral blood perforin and granzyme B gene expression measurements for prediction of acute rejection in kidney graft recipients
    Tania Simon
    Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, D 69120 Heidelberg, Germany
    Am J Transplant 3:1121-7. 2003
    ..Recipients with acute rejection (n = 17) had higher levels of perforin and granzyme B transcript on days 5-7, 8-10, 11-13, 17-19, 20-22, and 26-29, as compared to patients without ..
  12. pmc Characterisation of diverse PRF1 mutations leading to decreased natural killer cell activity in North American families with haemophagocytic lymphohistiocytosis
    S Molleran Lee
    J Med Genet 41:137-44. 2004
  13. ncbi Calcium-dependent plasma membrane binding and cell lysis by perforin are mediated through its C2 domain: A critical role for aspartate residues 429, 435, 483, and 485 but not 491
    Ilia Voskoboinik
    Cancer Immunology Program, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett St 8006 and The Walter and Eliza Hall Institute for Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Biol Chem 280:8426-34. 2005
    The lymphocyte pore-forming protein perforin is essential for maintaining immune homeostasis and for effective defense against intracellular pathogens...
  14. ncbi A single amino acid change A91V in perforin: a novel, frequent predisposing factor to childhood acute lymphoblastic leukemia?
    Alessandra Santoro
    Haematologica 90:697-8. 2005
    ..100 children with acute lymphoblastic leukemia (ALL) to assess the incidence of single amino acid change A91V in perforin. Heterozygous A91V was found in 12/100 patients and 5/127 controls (OR, 3.4; 95%CI: 1.15-9.95; p=0.014)...
  15. pmc Aberrant maturation of mutant perforin underlies the clinical diversity of hemophagocytic lymphohistiocytosis
    Kimberly A Risma
    Division of Allergy Immunology and Division of Hematology Oncology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    J Clin Invest 116:182-92. 2006
    Missense mutations in perforin, a critical effector of lymphocyte cytotoxicity, lead to a spectrum of diseases, from familial hemophagocytic lymphohistiocytosis to an increased risk of tumorigenesis...
  16. ncbi Human keratinocytes acquire cellular cytotoxicity under UV-B irradiation. Implication of granzyme B and perforin
    Hélène Hernandez-Pigeon
    INSERM U563, CPTP, Bat B, Pavillon Lefebvre, Place du Dr Baylac, Centre Hospitalier Universitaire Purpan, BP 3028, 31024 Toulouse Cedex 3, France
    J Biol Chem 281:13525-32. 2006
    ..Granzyme B (GrB) and perforin (PFN) are two proteins contained in granules and implicated in one of the mechanisms by which cytotoxic ..
  17. ncbi Cytotoxic T lymphocytes from cathepsin B-deficient mice survive normally in vitro and in vivo after encountering and killing target cells
    Katherine Baran
    Cancer Immunology Program, Peter MacCallum Cancer Centre, St Andrew s Place, East Melbourne 3002, Victoria, Australia
    J Biol Chem 281:30485-91. 2006
    ..protease cathepsin B has been proposed to protect cytotoxic T lymphocytes from the membrane-disruptive effects of perforin secreted during the execution phase of target cell death...
  18. ncbi Germline mutations of the perforin gene are a frequent occurrence in childhood anaplastic large cell lymphoma
    Sonia Cannella
    Pediatric Hematology Oncology, Ospedale dei Bambini G Di Cristina, Palermo, Italy
    Cancer 109:2566-71. 2007
    ..To assess the possible role of PRF1 mutations in ALCL, the authors screened a series of patients collected by the Associazione Italiana di Ematologia Oncologia Pediatrica (AIEOP)...
  19. doi Variations of the perforin gene in patients with multiple sclerosis
    G Cappellano
    Department of Medical Sciences, Interdisciplinary Research Center of Autoimmune Diseases, A Avogadro University of Eastern Piedmont, Novara, Italy
    Genes Immun 9:438-44. 2008
    b>Perforin is involved in cell-mediated cytotoxicity and mutations of its gene (PRF1) cause familial hemophagocytic lymphohistiocytosis (FLH2)...
  20. doi Flow cytometric detection of perforin upregulation in human CD8 T cells
    Adam R Hersperger
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Cytometry A 73:1050-7. 2008
    b>Perforin and granzymes work synergistically to induce apoptosis in target cells recognized by cytotoxic T lymphocytes...
  21. doi Decidual CD8+CD28- T cells express CD103 but not perforin
    Tamara Tilburgs
    Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, The Netherlands
    Hum Immunol 70:96-100. 2009
    ..Unlike peripheral CD8+CD28- T cells, decidual CD8+CD28- T cells do not express the cytolytic molecule perforin. The lack of perforin expression may indicate that decidual CD8+ T cells are not able to elicit a cytotoxic ..
  22. pmc Rapid up-regulation and granule-independent transport of perforin to the immunological synapse define a novel mechanism of antigen-specific CD8+ T cell cytotoxic activity
    George Makedonas
    Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Immunol 182:5560-9. 2009
    CTL are endowed with the ability to eliminate pathogens through perforin-mediated cytotoxic activity...
  23. pmc Perforin expression directly ex vivo by HIV-specific CD8 T-cells is a correlate of HIV elite control
    Adam R Hersperger
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS Pathog 6:e1000917. 2010
    ..We have recently discovered the ability of human CD8(+) T-cells to rapidly upregulate perforin--an essential molecule for cell-mediated cytotoxicity--following antigen-specific stimulation...
  24. doi Human perforin permeabilizing activity, but not binding to lipid membranes, is affected by pH
    Tilen Praper
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    Mol Immunol 47:2492-504. 2010
    The various steps that perforin (PFN), a critical mediator of innate immune response, undertakes to form a transmembrane pore remains poorly understood. We have used surface plasmon resonance (SPR) to dissect mechanism of pore formation...
  25. doi Mutations of perforin gene in Chinese patients with acute lymphoblastic leukemia
    Liyun Yang
    Department of Hematology, Beijing 301 Hospital, Beijing, China
    Leuk Res 35:196-9. 2011
    To address whether mutations and single nucleotide polymorphisms (SNPs) in perforin gene (PRF1) are correlated with acute lymphoblastic leukemia (ALL) in Chinese, we screened mutations in codon region of PRF1 in 111 ALL patients, and ..
  26. pmc Human perforin employs different avenues to damage membranes
    Tilen Praper
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    J Biol Chem 286:2946-55. 2011
    b>Perforin (PFN) is a pore-forming protein produced by cytotoxic lymphocytes that aids in the clearance of tumor or virus-infected cells by a mechanism that involves the formation of transmembrane pores...
  27. doi Gender-associated differences of perforin polymorphisms in the susceptibility to multiple sclerosis
    Montse Camiña-Tato
    Centre d esclerosi multiple de Catalunya, Unitat de Neuroimmunologia Clinica, Hospital Universitari Vall d Hebron, Barcelona, Spain
    J Immunol 185:5392-404. 2010
    ..In the current study, we investigated the perforin 1 gene (PRF1) as a candidate for multiple sclerosis (MS) susceptibility in the Spanish population...
  28. pmc Perforin rapidly induces plasma membrane phospholipid flip-flop
    Sunil S Metkar
    Department of Medicine, NorthShore University HealthSystems Research Institute, Evanston, Illinois, United States of America
    PLoS ONE 6:e24286. 2011
    ..through barrel stave pores generated in the plasma membrane of the target cell by the pore forming protein perforin (PFN) and mediate apoptotic as well as additional biological effects...
  29. ncbi Human perforin (PRF1) maps to 10q22, a region that is syntenic with mouse chromosome 10
    T M Fink
    Deutsches Krebsforschungszentrum, Schwerpunkt Angewandte Tumorvirologie, Heidelberg, Germany
    Genomics 13:1300-2. 1992
    b>Perforin (PRF1) is a cytolytic, channel-forming protein of cytolytic T cells, natural killer cells, and granulated metrial gland cells and plays a crucial role in the killer cell-mediated elimination of virally infected host cells, tumor ..
  30. ncbi Purification and characterization of a cytolytic pore-forming protein from granules of cloned lymphocytes with natural killer activity
    J D Young
    Cell 44:849-59. 1986
    A cytolytic pore-forming protein (PFP, perforin) was purified from isolated granules of cloned NK-like cytolytic cells, which showed an apparent Mr of 70-75 kd (reduced) and 62-66 kd (nonreduced)...
  31. ncbi The ninth component of complement and the pore-forming protein (perforin 1) from cytotoxic T cells: structural, immunological, and functional similarities
    J D Young
    Science 233:184-90. 1986
    The ninth component of complement (C9) and the pore-forming protein (PFP or perforin) from cytotoxic T lymphocytes polymerize to tubular lesions having an internal diameter of 100 A and 160 A, respectively, when bound to lipid bilayers...
  32. ncbi A proportion of patients with lymphoma may harbor mutations of the perforin gene
    Rita Clementi
    University of Pavia and Istituto di Ricovero e Cura a Carattere Scientifico, Policlinico San Matteo, Italy
    Blood 105:4424-8. 2005
    b>Perforin mutations have been demonstrated in a proportion of patients diagnosed with the familial form of hemophagocytic lymphohistiocytosis (HLH)...
  33. ncbi Perforin activity and immune homeostasis: the common A91V polymorphism in perforin results in both presynaptic and postsynaptic defects in function
    Ilia Voskoboinik
    Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Australia
    Blood 110:1184-90. 2007
    b>Perforin (PRF), a pore-forming protein expressed in cytotoxic lymphocytes, plays a key role in immune surveillance and immune homeostasis. The A91V substitution has a prevalence of 8% to 9% in population studies...
  34. pmc Functional assessment of perforin C2 domain mutations illustrates the critical role for calcium-dependent lipid binding in perforin cytotoxic function
    Ramon Urrea Moreno
    Division of Immunology, Hospital General Universitario Gregorio Maranon, Universidad Complutense de Madrid, Madrid, Spain
    Blood 113:338-46. 2009
    b>Perforin-mediated lymphocyte cytotoxicity is critical for pathogen elimination and immune homeostasis. Perforin disruption of target cell membranes is hypothesized to require binding of a calcium-dependent, lipid-inserting, C2 domain...
  35. doi Transcriptional profiles in urine during acute rejection, bacteriuria, CMV infection and stable graft function after renal transplantation
    A Øzbay
    Research Laboratory C, Department of Nephrology, Aarhus University Hospital, Skejby, Denmark
    Scand J Immunol 69:357-65. 2009
    ..We measured mRNA of perforin (PRF), granzyme B (GZMB) and granulysin (GNLY) normalized to cyclophilin B in urine specimens from 24 renal ..
  36. pmc Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells
    Jerome Thiery
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Immunol 12:770-7. 2011
    How the pore-forming protein perforin delivers apoptosis-inducing granzymes to the cytosol of target cells is uncertain...
  37. pmc Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2
    Bo Zheng
    Department of Bacteriology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
    Antimicrob Agents Chemother 53:735-47. 2009
    ..to erythromycin (Em), gentamicin (Gm), kanamycin (Km), tetracycline (Tc), and vancomycin (Van) and produced cytolysin (Hly/Bac) and a bacteriocin (Bac) active against E. faecalis strains...
  38. ncbi Continuous scale physical functional performance test: appropriateness for middle-aged adults with and without Parkinson's disease
    Thomas M Hearty
    School of Medicine, Physical Therapy Program, University of Colorado Health Science Center, Denver, Colorado, USA
    J Neurol Phys Ther 31:64-70. 2007
    ..One potential measure is the Continuous Scale Physical Functional Performance Test (CS-PFP), which has been normalized and validated to older adults but performance is unknown for adults younger than the ..
  39. ncbi Hair analysis for drugs of abuse. XIX. Determination of ephedrine and its homologs in rat hair and human hair
    Y Nakahara
    National Institute of Health Sciences, Tokyo, Japan
    J Chromatogr B Biomed Sci Appl 700:83-91. 1997
    ..From the results comparing three GC-MS conditions, PFP-derivatives separated with a temperature gradient of 20 degrees C/min from 60 degrees C to 280 degrees C gave the ..
  40. ncbi Protein sequence analysis, cloning, and expression of flammutoxin, a pore-forming cytolysin from Flammulina velutipes. Maturation of dimeric precursor to monomeric active form by carboxyl-terminal truncation
    Toshio Tomita
    Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai 981 8555, Japan
    J Biol Chem 279:54161-72. 2004
    Flammutoxin (FTX), a 31-kDa pore-forming cytolysin from Flammulina velutipes, is specifically expressed during the fruiting body formation...
  41. pmc Survival of Enterococcus faecalis in mouse peritoneal macrophages
    C R Gentry-Weeks
    Department of Microbiology, Colorado State University, Fort Collins, Colorado, USA
    Infect Immun 67:2160-5. 1999
    ..All E. faecalis isolates were serum passaged to enhance the production of cytolysin. E. faecalis 418, FA2-2(pAM714), and FA2-2(pAM771) survived at a significantly higher level (P = 0...
  42. pmc Saxitoxin puffer fish poisoning in the United States, with the first report of Pyrodinium bahamense as the putative toxin source
    Jan H Landsberg
    Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, St Petersburg, Florida 33701, USA
    Environ Health Perspect 114:1502-7. 2006
    From January 2002 to May 2004, 28 puffer fish poisoning (PFP) cases in Florida, New Jersey, Virginia, and New York were linked to the Indian River Lagoon (IRL) in Florida...
  43. pmc Profile of Salmonella enterica subsp. enterica (subspecies I) serotype 4,5,12:i:- strains causing food-borne infections in New York City
    Alice Agasan
    New York City Department of Health, New York, New York, USA
    J Clin Microbiol 40:1924-9. 2002
    ..PFGE), the strains showed 11 or 12 resolvable genomic fragments with 18 banding patterns and three PFGE profile (PFP) clusters (i.e., PFP/A, PFP/B, and PFP/C)...
  44. ncbi Factor VIIa-mediated tenase function on activated platelets under flow
    M S Goel
    Institute for Medicine and Engineering, Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA, USA
    J Thromb Haemost 2:1402-10. 2004
    ..Tissue factor (TF) and/or active factor (F)VIIa may be stored inside resting platelets...
  45. doi Occurrence and characteristics of the cytolysin A gene in Shigella strains and other members of the family Enterobacteriaceae
    Christine von Rhein
    Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Klinikum der Johann Wolfgang Goethe Universitat, Paul Ehrlich Strasse 40, Frankfurt am Main, Germany
    FEMS Microbiol Lett 287:143-8. 2008
    b>Cytolysin A (ClyA, HlyE, SheA) is a hemolytic pore-forming toxin found in Escherichia coli and Salmonella enterica serovars Typhi and Paratyphi A...
  46. pmc The Vibrio cholerae cytolysin promotes chloride secretion from intact human intestinal mucosa
    Lucantonio Debellis
    Department of General and Environmental Physiology, University of Bari, Bari, Italy
    PLoS ONE 4:e5074. 2009
    ....
  47. ncbi [Association of peripheral facial paralysis in patients with human immunodeficiency virus infection]
    P Casanova Sotolongo
    , Beira, Mozambique
    Rev Neurol 32:327-30. 2001
    ..The objective of this study is to show the possible association of peripheral facial paralysis (PFP) in persons who test positive for HIV, which because of the characteristics of outpatient management is important ..
  48. pmc Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes
    Sita R Dewamitta
    Department of Microbiology, Kyoto University Graduate School of Medicine, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Infect Immun 78:1884-94. 2010
    Listeriolysin O (LLO), an hly-encoded cytolysin of Listeria monocytogenes, plays an essential role in the entry of L. monocytogenes into the host cell cytoplasm. L...
  49. ncbi Facial motor cortex plasticity in patients with unilateral peripheral facial paralysis
    S Yildiz
    Department of Clinical Neurophysiology, Medical School Hospital, Ege University, Izmir, Turkey
    NeuroRehabilitation 22:133-40. 2007
    ..area expands towards the area of the perioral facial motor cortex in patients with peripheral facial paralysis (PFP) and in hemifacial spasm cases treated with botulinum toxin...
  50. ncbi Determination of hair polyamines as N-ethoxycarbonyl-N-pentafluoropropionyl derivatives by gas chromatography-mass spectrometry
    M H Choi
    Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul, South Korea
    J Chromatogr A 897:295-305. 2000
    ..EOC) reaction of amino functions in aqueous solutions combined with subsequent pentafluoropropionyl (PFP) derivatization of the remaining active hydrogen atoms for the direct analysis by GC-SIM-MS...
  51. pmc Global genomic analysis of Pseudomonas savastanoi pv. savastanoi plasmids
    Isabel Pérez-Martínez
    Area de Genetica, Universidad de Malaga, Facultad de Ciencias, Campus de Teatinos s n, 29071 Malaga, Spain
    J Bacteriol 190:625-35. 2008
    ..Additionally, most strains contained at least one plasmid (designated non-PFP) that did not hybridize to the repA gene of pPT23A...
  52. ncbi Occurrence of pathogenic vibrios in coastal areas of France
    D Hervio-Heath
    IFREMER, DEL MP, Laboratoire de Microbiologie, Plouzane, France
    J Appl Microbiol 92:1123-35. 2002
    ..This study was carried out to investigate the occurrence of potentially pathogenic species of Vibrio in French marine and estuarine environments...
  53. ncbi Interaction of the Vibrio cholerae cytolysin (VCC) with cholesterol, some cholesterol esters, and cholesterol derivatives: a TEM study
    J Robin Harris
    Institute of Zoology, University of Mainz, 55099 Mainz, Germany
    J Struct Biol 139:122-35. 2002
    The Vibrio cholerae cytolysin (VCC) 63-kDa monomer has been shown to interact in aqueous suspension with cholesterol microcystals to produce a ring/pore-like heptameric oligomer approximately 8 nm in outer diameter...
  54. ncbi Construction of an immunotoxin with the pore forming protein StI and ior C5, a monoclonal antibody against a colon cancer cell line
    M Tejuca
    Centro de Estudios de Proteínas y Departamento de Bioquímica, Facultad de Biologia, Universidad de La Habana, Cuba
    Int Immunopharmacol 4:731-44. 2004
    Sticholysin I (StI), a potent cytolysin isolated from the sea anemone Stichodactyla helianthus, was linked to the monoclonal antibody (mAb) ior C5...
  55. pmc Functional and phylogenetic characterization of Vaginolysin, the human-specific cytolysin from Gardnerella vaginalis
    Shari E Gelber
    Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Bacteriol 190:3896-903. 2008
    ..vaginalis produces a toxin (vaginolysin [VLY]) that is a member of the cholesterol-dependent cytolysin (CDC) family, most closely related to intermedilysin from Streptococcus intermedius...
  56. doi MLST analysis reveals a highly conserved core genome among poultry isolates of Clostridium septicum
    Anthony P Neumann
    Agtech Products, Inc, W227 N752 Westmound Drive, Waukesha, WI 53186, USA
    Anaerobe 15:99-106. 2009
    ..Disease is primarily facilitated by secretion of numerous toxic products including a lethal pore-forming cytolysin. Spontaneously occurring clostridial myonecrosis involving C...
  57. ncbi Serratia marcescens hemolysin (ShlA) binds artificial membranes and forms pores in a receptor-independent manner
    R Hertle
    Microbiology and Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    J Membr Biol 189:1-14. 2002
    Both the inactive and active conformations of the hemolysin/cytolysin of Serratia marcescens (ShlA) binds membranes of erythrocytes, but only active ShlA is able to form pores. ShlA is unable to lyse prokaryotic membranes...
  58. ncbi Effect of sphingomyelin and cholesterol on the interaction of St II with lipidic interfaces
    Diana Martinez
    Facultad de Biologia, Universidad de La Habana, Centro de Estudio de Proteínas, Calle 25 No 455, CP 10400, Cuba
    Toxicon 49:68-81. 2007
    Sticholysin II (St II) is a cytolysin produced by the sea anemone Stichodactyla helianthus, characterized by forming oligomeric pores in natural and artificial membranes...
  59. ncbi Structural analysis of human specific cytolysin intermedilysin aiming application to cancer immunotherapy
    Kazuto Ohkura
    Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, Japan
    Anticancer Res 24:3343-53. 2004
    Intermedilysin (ILY) is a human specific cytolysin secreted by Streptococcus intermedius...
  60. ncbi Bladder reconstruction using a collagen patch prefabricated within the omentum
    Kazunori Hattori
    Department of Urology, University of Tsukuba, Tsukuba, Japan
    Int J Urol 13:529-37. 2006
    ..The aim of the study is to evaluate tissue regeneration of the reconstructed bladder and the effect of prefabricating the collagen patch within the omentum...
  61. ncbi Activated leukocytes and endothelial cells enhance retention of ultrasound contrast microspheres containing perfluoropropane in inflamed venules
    Takanori Yasu
    Department of Bioengineering, University of California San Diego, La Jolla, CA 92093 0412, USA
    Int J Cardiol 98:245-52. 2005
    To characterize the flow dynamics of albumin ultrasound contrast microspheres containing perfluoropropane (PFP) in normal and inflamed microvasculature.
  62. doi Functional movement training for recurrent low back pain: lessons from a pilot randomized controlled trial
    Margaret L Schenkman
    Department of Physical Medicine and Rehabilitation, University of Colorado Denver, Aurora, CO, USA
    PM R 1:137-46. 2009
    ....
  63. ncbi Abnormalities induced in reciprocal inhibition between orbicularis oculi and levator palpebrae following peripheral facial palsy
    J M Pardal-Fernández
    Electromyography Unit, Department of Clinical Neurophysiology, University General Hospital, Albacete, Spain
    Electromyogr Clin Neurophysiol 49:299-304. 2009
    Peripheral Facial palsy (PFP) is generally considered a benign condition with good recovery and no sequelae...
  64. doi Singlet-singlet energy transfer in self-assembled systems of the cationic poly{9,9-bis[6-N,N,N-trimethylammonium)hexyl]fluorene-co-1,4-phenylene} with oppositely charged porphyrins
    Sara M Pinto
    Departamento de Quimica, Universidade de Coimbra, 3004 535 Coimbra, Portugal
    J Phys Chem B 113:16093-100. 2009
    ..conjugated polyelectrolyte, poly{9,9-bis[6-N,N,N-trimethylammonium)hexyl]fluorene-co-1,4-phenylene} dibromide (HTMA-PFP), and three, oppositely charged meso-tetrakis-phenylporphyrinsulfonates in buffered (pH = 9...
  65. ncbi Clinical data and cerebrospinal fluid findings in Lyme meningitis versus aseptic meningitis
    David Tuerlinckx
    Department of Paediatrics, Cliniques Universitaires de Mont Godinne, Universite Catholique de Louvain, Mont Godinne, 5530 Yvoir, Belgium
    Eur J Pediatr 162:150-3. 2003
    ..with AM and 14 with LM, amongst which 7 presented with isolated LM and 7 with associated peripheral facial palsy (PFP)...
  66. ncbi Dependency of caspase-1 activation induced in macrophages by Listeria monocytogenes on cytolysin, listeriolysin O, after evasion from phagosome into the cytoplasm
    Hideki Hara
    Department of Microbiology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    J Immunol 180:7859-68. 2008
    Listeriolysin O (LLO), an hly-encoded cytolysin from Listeria monocytogenes, plays an essential role in the entry of this pathogen into the macrophage cytoplasm and is also a key factor in inducing the production of IFN-gamma during the ..
  67. pmc Vibrio cholerae cytolysin causes an inflammatory response in human intestinal epithelial cells that is modulated by the PrtV protease
    Gangwei Ou
    Department of Clinical Microbiology Immunology, Umea University, Umea, Sweden
    PLoS ONE 4:e7806. 2009
    ..The aim was to identify the secreted component(s) of V. cholerae that induces an epithelial inflammatory response and to define whether it is a substrate for PrtV...
  68. ncbi Clinical and electromyographic study of lateral preference in mastication in patients with longstanding peripheral facial paralysis
    Adriana Rahal
    Departamento de Otorrinolaringologia, Faculdade de Medicina da Universidade de Sao Paulo, SP, Brasil
    Int J Orofacial Myology 35:19-32. 2009
    Peripheral facial paralysis (PFP) usually affects the facial nerve in part or in whole on one side of the face...
  69. ncbi Development of the perforating pathway: an ipsilaterally projecting pathway between the medial septum/diagonal band of Broca and the cingulate cortex that intersects the corpus callosum
    T Shu
    Department of Anatomy and Neurobiology, School of Medicine, The University of Maryland, Baltimore, Baltimore, Maryland 21201, USA
    J Comp Neurol 436:411-22. 2001
    The perforating pathway (PFP) intersects the corpus callosum perpendicularly at the midline in the dorsoventral axis...
  70. ncbi Crystal structure of the Vibrio cholerae cytolysin (VCC) pro-toxin and its assembly into a heptameric transmembrane pore
    Rich Olson
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 W 168th Street, New York, NY 10032, USA
    J Mol Biol 350:997-1016. 2005
    Pathogenic Vibrio cholerae secrete V. cholerae cytolysin (VCC), an 80 kDa pro-toxin that assembles into an oligomeric pore on target cell membranes following proteolytic cleavage and interaction with cell surface receptors...
  71. pmc Efficient gene transfer and targeted mutagenesis in Fusobacterium nucleatum
    Susan Kinder Haake
    Section of Periodontics, UCLA School of Dentistry, Los Angeles, CA 90095 1668, USA
    Plasmid 55:27-38. 2006
    ..We previously reported construction of a shuttle plasmid, pHS17, using the native fusobacterial plasmid pFN1 and an erythromycin resistance cassette. However, the host range of pHS17 was restricted to F...
  72. pmc Enhanced immunity to Plasmodium falciparum circumsporozoite protein (PfCSP) by using Salmonella enterica serovar Typhi expressing PfCSP and a PfCSP-encoding DNA vaccine in a heterologous prime-boost strategy
    Magaly Chinchilla
    Center for Vaccine Development, University of Maryland, 685 W Baltimore Street, HSF I, Room 480, Baltimore, MD 21201, USA
    Infect Immun 75:3769-79. 2007
    ..version of Plasmodium falciparum circumsporozoite surface protein (tCSP) fused to Salmonella serovar Typhi cytolysin A (ClyA) were constructed as a first step in the development of a preerythrocytic malaria vaccine...
  73. ncbi Vibrio cholerae cytolysin is essential for high enterotoxicity and apoptosis induction produced by a cholera toxin gene-negative V. cholerae non-O1, non-O139 strain
    Hector Alex Saka
    Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Centro de Investigaciones en Bioquímica e Inmunología ClBICI CONICET, Cordoba, Argentina
    Microb Pathog 44:118-28. 2008
    ..V. cholerae cytolysin (VCC) is a pore-forming exotoxin encoded in the hlyA gene of V...
  74. pmc Antibody-based detection and inhibition of vaginolysin, the Gardnerella vaginalis cytolysin
    Tara M Randis
    Department of Pediatrics, Columbia University, New York, NY, USA
    PLoS ONE 4:e5207. 2009
    ..Furthermore, VLY antiserum functions as an inhibitor of VLY-CD59 interaction, mitigating cell lysis. These strategies may have a potential role in the diagnosis and treatment of BV...
  75. doi Synthesis of hetero-telechelic alpha,omega bio-functionalized polymers
    Peter J Roth
    Institute of Organic Chemistry, University of Mainz, Duesbergweg 10 14, 55099 Mainz, Germany
    Biomacromolecules 11:238-44. 2010
    ..14) with a pentafluorophenyl (PFP) activated ester and a dithioester end group...
  76. ncbi Circulating levels and ex vivo production of beta-chemokines, interferon gamma, and interleukin 2 in advanced human immunodeficiency virus type 1 infection: the effect of protease inhibitor therapy
    A De Luca
    Institute of Clinical Infectious Diseases, Catholic University, Rome, Italy
    AIDS Res Hum Retroviruses 16:835-43. 2000
    ..We studied platelet-free plasma (PFP) levels and ex vivo production of cytokines and beta-chemokines at different HIV disease stages and the influence ..
  77. ncbi Using peer feedback to improve handwashing and glove usage among Thai health care workers
    W Moongtui
    Faculty of Nursing, Chiangmai University, Thailand
    Am J Infect Control 28:365-9. 2000
    ..The purpose of this study was to evaluate the effectiveness of a peer feedback program (PFP) on handwashing and glove wearing (HW/GW) among Thai health care workers...
  78. doi Peptide-mediated energy transfer between an anionic water-soluble conjugated polymer and Texas red labeled DNA for protease and nuclease activity study
    Yong Zhang
    Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, Singapore 117576
    Anal Chem 81:3731-7. 2009
    ..peptide-mediated energy transfer between poly(9,9-bis(4'-sulfonatobutyl)fluorene-alt-1,4-phenylene) sodium salt (PFP-SO(3)Na) and Texas red (TR)-labeled single-stranded DNA (ssDNA), we develop a homogeneous assay for detection and ..
  79. pmc Mobilization and prevalence of a Fusobacterial plasmid
    Brianna M Claypool
    Sections of Periodontics, UCLA School of Dentistry, Los Angeles, CA 90095 1668, USA
    Plasmid 63:11-9. 2010
    ..Several plasmids isolated from F. nucleatum, including pFN1, harbor relaxase gene homologs that may function in plasmid mobilization...
  80. doi Orthogonal array design for antidepressant compatibility of polysaccharides from Banxia-Houpu decoction, a traditional Chinese herb prescription in the mouse models of depression
    Li Tao Yi
    State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China
    Arch Pharm Res 32:1417-23. 2009
    ..composed of rhizome pinelliae (PRP), magnolia bark (PMB), poria (PPO), ginger rhizome (PGR) and folium perillae (PFP) by a L(16)(2(5)) orthogonal array design with a two-factor interaction in the tail suspension and forced swimming ..
  81. doi The cholesterol-dependent cytolysin family of gram-positive bacterial toxins
    Alejandro P Heuck
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Subcell Biochem 51:551-77. 2010
    ..Recent studies support the concept that there is a dynamic interplay between the cholesterol-binding domain of the CDCs and the excess of cholesterol molecules in the target membrane...
  82. ncbi Serum neutralizing antibody titers of seropositive chimpanzees immunized with vaccines coformulated with natural fusion and attachment proteins of respiratory syncytial virus
    G E Hancock
    Department of Immunology Research, Wyeth Lederle Vaccines, West Henrietta, NY 14586 9728
    J Infect Dis 181:1768-71. 2000
    Subunit vaccines formulated with purified fusion proteins from the A2 (PFP-2) or attenuated 248/404 (PFP-3) strains of respiratory syncytial virus (RSV) were evaluated, either alone or in combination with native attachment (G) protein, ..
  83. ncbi Profilin 1 obtained by proteomic analysis in all-trans retinoic acid-treated hepatocarcinoma cell lines is involved in inhibition of cell proliferation and migration
    Nan Wu
    Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P R China
    Proteomics 6:6095-106. 2006
    ..We selected the up-regulated protein, profilin 1 (PFN1), for further studies. The PFN1 expression was increased in response to ATRA in a dose- and time-dependent manner...
  84. ncbi Influences of the recombinant artificial cell adhesive proteins on the behavior of human umbilical vein endothelial cells in serum-free culture
    Akiko Ishii-Watabe
    Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo, Japan
    Biologicals 35:247-57. 2007
    ..The recombinant proteins used were Pronectin F (PF), Pronectin F PLUS (PFP), Pronectin L (PL), Retronectin (RN), and Attachin (AN). HUVECs adhered more efficiently on PF or PFP than on FN...
  85. doi Fibrosis and adventitious bursae in plantar fat pad of forefoot: MR imaging findings in asymptomatic volunteers and MR imaging-histologic comparison
    Ueli Studler
    Department of Radiology, Orthopedic University Hospital Balgrist, Forchstrasse 340, Ch 8008 Zurich, Switzerland
    Radiology 246:863-70. 2008
    To retrospectively evaluate plantar fat pad (PFP) signal intensity alterations in magnetic resonance (MR) imaging studies of asymptomatic volunteers and to compare PFP alterations with histopathologic findings in cadavers and patients.
  86. ncbi [Effectiveness of physiotherapy treatment in peripheral facial palsy. A systematic review]
    R La Touche
    Departamento de Fisioterapia, Universidad San Pablo CEU, Facultad de Medicina, Madrid, Espana
    Rev Neurol 46:714-8. 2008
    To make a review and analysis of articles that used physical therapy methods for peripheral facial paralysis (PFP).
  87. doi Outer membrane vesicles of Vibrio vulnificus deliver cytolysin-hemolysin VvhA into epithelial cells to induce cytotoxicity
    Young Ran Kim
    Clinical Vaccine R and D Center, Chonnam National University Medical School, Gwangju 501 746, Republic of Korea
    Biochem Biophys Res Commun 399:607-12. 2010
    ..Previously, we reported that substantial amounts of V. vulnificus cytolysin-hemolysin (VvhA) are produced in vivo during the bacterial infection in mice and that this cytotoxin, in ..
  88. pmc Characterization of a streptococcal cholesterol-dependent cytolysin with a lewis y and b specific lectin domain
    Stephen Farrand
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Biochemistry 47:7097-107. 2008
    ..Hence, LLY represents a new class of CDC whose pore-forming mechanism is modulated by a glycan-binding domain...
  89. pmc Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems
    S K Haake
    Divisions of Associated Clinical Sciences, UCLA School of Dentistry, Los Angeles, California 90095 1668, USA
    J Bacteriol 182:1176-80. 2000
    Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F...
  90. ncbi Shikonin production by p-fluorophenylalanine resistant cells of Lithospermum erythrorhizon
    V P Bulgakov
    The Institute of Biology and Soil Science of the Far East Branch of Russian Academy of Sciences, 690022, Vladivostok, Russia
    Fitoterapia 72:394-401. 2001
    ..A selection of cell aggregates of BK-39 culture on a medium containing p-fluorophenylalanine (PFP) yields a cell line possessing a higher resistance to the inhibitor than the initial culture...
  91. ncbi Anaerobic power and physical function in strength-trained and non-strength-trained older adults
    Jill M Slade
    Department of Exercise Science, University of Georgia, Athens 30602, USA
    J Gerontol A Biol Sci Med Sci 57:M168-72. 2002
    ....
  92. pmc Autonomous expression of the slo gene of the bicistronic nga-slo operon of Streptococcus pyogenes
    Dragutin J Savic
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Infect Immun 70:2730-3. 2002
    A recent model for cytolysin-mediated translocation in Streptococcus pyogenes proposes that NAD-glycohydrolase is translocated through streptolysin O-generated pores into a host cell (J. Madden, N. Ruiz, and M...
  93. ncbi Contribution of histidine residues to oligomerization of theta-toxin (perfringolysin O), a cholesterol-binding cytolysin
    M Nakamura
    Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan
    Biosci Biotechnol Biochem 63:1640-3. 1999
    ..These results suggest that histidine residues of theta-toxin contribute their share to cytolysis, especially the oligomerization process...
  94. ncbi Cross-reactivity and inhibition of haemolysis by polyclonal antibodies raised against St II, a cytolysin from the sea anemone Stichodactyla helianthus
    María Cristina Pico
    Centro de Estudio de Proteínas, Facultad de Biologia, Universidad de La Habana, Calle 25 No 455, entre J e I Vedado, Ciudad Habana 10400, Cuba
    Toxicon 43:167-71. 2004
    ..the oil constituent of FA neutralized the St II haemolytic activity, immunizations with St II-Freund's emulsions were carried out with the inactivated cytolysin. Purified anti-St II IgG also neutralized the St II haemolytic activity.
  95. pmc The apoptotic response to pneumolysin is Toll-like receptor 4 dependent and protects against pneumococcal disease
    Amit Srivastava
    Division of Infectious Diseases, Department of Medicine, Children s Hospital, Boston, MA 02115, USA
    Infect Immun 73:6479-87. 2005
    Pneumolysin, the cholesterol-dependent cytolysin of Streptococcus pneumoniae, induces inflammatory and apoptotic events in mammalian cells...
  96. ncbi MAPkinase gene expression, as determined by microarray analysis, distinguishes uncomplicated from complicated reconstitution after major surgical trauma
    E Marion Schneider
    Section of Experimental Anesthesiology, Department of Clinical Anesthesiology, University Clinic Ulm, Steinhoevelstrasse 9, 89075 Ulm, Germany
    Ann N Y Acad Sci 1090:429-44. 2006
    ..NK) cell maturation (SCYA14 [HCC-1]), and activators of the lymphoid compartment (TNFRSF7 [CD27], CD3zeta and perforin [PRF1]) were increased...
  97. ncbi Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria
    Takamasa Nomura
    Department of Microbiology, Kyoto University Graduate School of Medicine, Kyoto 606 8501, Japan
    Microbiology 153:2250-8. 2007
    Listeriolysin O (LLO), a member of the cholesterol-dependent cytolysin (CDC) family, is a major virulence factor of Listeria monocytogenes and contributes to bacterial escape from intracellular killing of macrophages...
  98. doi Effect of iron on cytolysin A expression in Salmonella enterica serovar Typhi
    Jinghua Cui
    Department of Microbiology, Chonnam National University Medical School, Gwangju 501 746, Republic of Korea
    J Microbiol 47:479-85. 2009
    Previously, a novel protein ClyA (Cytolysin A) has been identified in Escherichia coli K-12, Salmonella enterica serovars Typhi and Paratyphi A and Shigella. Salmonella spp...
  99. ncbi Familial hemophagocytic lymphohistiocytosis in a 6-week-old male infant
    Gordana Jakovljevic
    Department of Hematology and Oncology, Pediatric Clinic, Zagreb Children s Hospital, Zagreb, Croatia
    Coll Antropol 34:631-4. 2010
    ..Familiar HLH is generally a progressive and fatal disease. Early diagnosis with molecular genetic analysis and chemoimmunotherapy followed by hematopoietic stem-cell transplantation is the best approach...
  100. doi Validation of a mutant of the pore-forming toxin sticholysin-I for the construction of proteinase-activated immunotoxins
    David Penton
    Faculty of Biology, Center for Protein Studies, University of Havana, Calle 25 455 e J e I, Vedado, Ciudad de La Habana, Cuba
    Protein Eng Des Sel 24:485-93. 2011
    ..tumour proteinase-activated ITs using a cysteine mutant at the membrane binding region of sticholysin-I (StI), a cytolysin isolated from the sea anemone Stichodactyla helianthus...
  101. doi Perforin-mediated cytotoxicity in non-ST elevation myocardial infarction
    G Laskarin
    Division of Cardiology, Hospital for Medical Rehabilitation of Hearth and Lung Diseases and Rheumatism Thalassotherapia Opatija, Opatija, Croatia
    Scand J Immunol 74:195-204. 2011
    The aim of this investigation was to examine the role of perforin (P)-mediated cytotoxicity in the dynamics of tissue damage in patients with non-ST-segment elevation myocardial infarction (NSTEMI) treated with anti-ischaemic drugs...

Research Grants54

  1. Epidemiology of Patellofemoral Pain Syndrome: Identifying Gender Specific Risk Fa
    MICHELLE CLARA BOLING; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Patellofemoral pain syndrome (PFP) is one of the most common causes of knee pain, affecting approximately 25% of the physically active population, with females being 2-3 times more likely to develop ..
  2. Mechanisms of host defense against membrane damage by pore-forming toxins
    MARY O'RIORDAN; Fiscal Year: 2013
    ..Caspase-7 activation is triggered during Listeria monocytogenes infection by the cholesterol dependent cytolysin, Listeriolysin O (LLO)...
  3. Mechanism of Cell Membrane Targeting by Vibrio Cholerae Cytolysin
    RICHARD A OLSON; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Vibrio cholerae Cytolysin (VCC) is a potent pore-forming toxin (PFT) that attacks human cells, although the specific targets of the toxin and role in pathogenesis are unknown...
  4. Prevention of Acute Lung Injury by IL-6 activated STAT3
    Ana Moran; Fiscal Year: 2010
    ....
  5. NANCY ELIZABETH FREITAG; Fiscal Year: 2016
    ..exhibit reduced secretion and activity of at least two key Lm virulence gene products, the cholesterol-dependent cytolysin listeriolysin O (LLO) and the broad specificity phospholipase PC-PLC...
  6. Post-translation regulation of Listeria monocytogenes virulence factors
    NANCY ELIZABETH FREITAG; Fiscal Year: 2010
    ..exhibit reduced secretion and activity of at least two key Lm virulence gene products, the cholesterol-dependent cytolysin listeriolysin O (LLO) and the broad specificity phospholipase PC-PLC...
  7. Michael G Caparon; Fiscal Year: 2014
    ..In this project, we have found that SLO plays a central role in the Cytolysin-Mediated Translocation (CMT) pathway, a novel pathway that acts to translocate the S...
  8. SpyA, a novel S. pyogenes ADP-ribosyltransferase
    STEPHEN L MOSELEY; Fiscal Year: 2010
    ..We proposed that SpyA gains entrance into the eukaryotic cell using a pore produced by the cholesterol dependent cytolysin SLO...
  9. ADAM JONATHAN RATNER; Fiscal Year: 2015
    ..We have recently characterized vaginolysin (VLY), a cholesterol-dependent cytolysin from Gardnerella vaginalis (a bacterial species present on the vaginal mucosa in the setting of BV and thought ..
  10. MATTHEW EUGENE PIPKIN; Fiscal Year: 2015
    ..effector and memory cytotoxic T lymphocytes (CTL) that control infections and tumors using the cytotoxic proteins perforin (Prf1), granzyme B (Gzmb), and effector cytokines such as Ifng...
  11. ROLE OF AEROMONAS HYDROPHILA VIRULENCE FACTORS
    CLIFFORD HOUSTON; Fiscal Year: 1992
    ..hydrophila mediated diseases. We have termed one of these toxins "cholera-toxin crossreactive (CTC) cytolysin" because of its ability to react with cholera antitoxin and cause fluid accumulation in rabbit intestinal loops ..
  12. Medial Collapse Movement Impairments associated with Patellofemoral Pain
    GRETCHEN SALSICH; Fiscal Year: 2009
    ..faults that define medial collapse, however, have not been identified in individuals with patellofemoral pain (PFP)...
  13. AJIT MOHAN WORTHEN CHAUDHARI; Fiscal Year: 2014
    ..of people in the US to battle obesity and maintain a healthy lifestyle, yet injuries such as patellofemoral pain (PFP) and iliotibial band syndrome (ITBS) often force many runners to quit the activity all together...
  14. RYAN MICHAEL MCCORMACK; Fiscal Year: 2016
    ..It was revealed that this mRNA encodes a protein domain, MACPF, which predicts a pore-forming, perforin-like molecule...
  15. Michael B Blank; Fiscal Year: 2016
    ..Previously we have conducted a Phase II trial of PATH for Positives (PFP) in which we observed broad and far-reaching effects of an intervention utilizing a nurse health navigator (NHN) ..
  16. MECHANISM OF PATHOGENESIS OF MYCOPLASMA PULMONIS
    F Minion; Fiscal Year: 1991
    ..virulence may be related to the activities of specific membrane proteins, specifically membrane bound hemolysis (cytolysin) and a membrane bound endonuclease...
  17. Zuoshang Xu; Fiscal Year: 2014
    ..Our preliminary experiments in cultured cells suggest that the PFN1 mutants can form intracellular aggregates, dominantly disrupt actin polymerization and growth cone morphology, ..
  18. Michael S Gilmore; Fiscal Year: 2016
    ..that one of the factors that makes hospital isolate of Enterococcus faecalis more virulent is a toxin termed "cytolysin." The E. faecalis cytolysin has antibacterial properties, as well as being a cytolytic toxin for human cells...
  19. Molecular Mechanisms of Natural Killer Cell Cytokine-Activation
    Todd A Fehniger; Fiscal Year: 2013
    ..Ley's laboratory identified two critical cytotoxic molecules, granzyme B (GzmB) and perforin (Prf1), that are post-transcriptionally regulated in NK cells...
  20. FDA FERN Laboratory Accreditation Project Grant(FDA-FLAP Grant)
    BRENDA SNODGRASS; Fiscal Year: 2012
    ..five years, and will follow the recommendations of the Partnership for Food Protection - Laboratory Task Group (PFP-LTG). The PFP is a joint multi-federal agency and state partnership, hosted by the FDA...
  21. NANCY E GLASS; Fiscal Year: 2016
    ..for example, through active participation in an adult animal husbandry microfinance program, Pigs for Peace (PFP)...
  22. ROBERTO ARIEL GOMEZ; Fiscal Year: 2016
    ..Pilot and feasibility projects (PFP) within the general theme of the program will be solicited from the Universities at large and the ASPN with the ..
  23. DEVELOPMENT OF NEW SCID MOUSE MODELS FOR AIDS RESEARCH
    Leonard Shultz; Fiscal Year: 1999
    ..allele; (b) the MHC class II (I-A-beta) disrupted allele; (c) the invariant chain (Ii) disrupted allele; (d) the perforin (Pfp) disrupted allele; and (e) three linked human transgenes encoding: stem cell factor, interleukin-3, and ..
  24. Daisuke Kihara; Fiscal Year: 2014
    ..The sequence-based methods we will develop and employ include our novel function prediction methods, PFP and its variant, which are shown to have higher sensitivity and higher function assignment coverage than ..
  25. Diabetes Research &Training Center
    Jean E Schaffer; Fiscal Year: 2012
    ..members, a similar increase in NIH funding for diabetes-related research, a vibrant Pilot and Feasibility Program (PFP), significant productivity in terms of total number of publications attributable to the DRTC Cores (872), and ..
  26. INTERACTION OF ENTEROCOCCUS FAECALIS WITH MACROPHAGES
    Claudia Gentry Weeks; Fiscal Year: 2003
    ..faecalis blood isolates, fecal isolates, and isogenic mutants lacking either cytolysin, gelatinase (protease) aggregation substance, UV resistance, or surface carbohydrate for the ability to survive ..
  27. CYTOLYSINS OF HAEMOPHILUS DUCREYI
    Patricia Totten; Fiscal Year: 1999
    ..mutagenesis of cytolytic strain 35000 resulted in nine transposon mutants with three phenotypes: two with no cytolysin expression, one with reduced cytolysin expression, and six with enhanced cytolysin expression...
  28. GENETIC SYSTEMS FOR FUSOBACTERIUM NUCLEATUM
    Susan Kinder Haake; Fiscal Year: 2002
    ..The Principal Investigator's laboratory has recently isolated and characterized a F. nucleatum plasmid, pFN1. In this proposal, the hypothesis to be tested is that pFN1 can be used to develop a system of gene transfer as ..
  29. Partha Roy; Fiscal Year: 2016
    ..applicant): The overall goal of the proposed project is to investigate the functional significance of profilin-1 (Pfn1 - a cytoskeleton regulatory protein) downregulation in breast cancer...
  30. SEX PHEROMONE INDUCED PLASMID TRANSFER
    DON CLEWELL; Fiscal Year: 2002
    ..faecalis and probably contribute significantly to the dissemination of antibiotic resistance and cytolysin (e.g. hemolysin/bacteriocin) production in this species...
  31. The Pore-forming Toxin of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2003
    ..Our data show that expression of the pore-forming toxin/cytolysin by intracellular L...
  32. SALMOLYSIN MEDIATED VIRULENCE IN SALMONELLA
    STEPHEN LIBBY; Fiscal Year: 1999
    The long term goal of this proposal is to understand the role salmolysin, a cytolysin identified in Salmonella, plays in Salmonella virulence...
  33. Enterococcus cytolysin: Regulation and structure
    Michael Gilmore; Fiscal Year: 2007
    ..the factors that has been thoroughly documented to contribute to the virulence of Enterococcus faecalis is the cytolysin. The cytolysin is a structurally novel toxin that consists of two dissimilar, small subunits...
  34. Regulation of cytolysin production in Enterococcus feacalis
    Shonna McBride; Fiscal Year: 2009
    ..One such factor that has been recognized for its contribution to the virulence of E. faecalis is the toxin, cytolysin. Cytolysin is a novel toxin, capable of lysing a wide variety of cell types and organisms - both bacterial and ..
  35. Molecular Mechanisms of anti Tumor and anti Bacterial Cytotoxicity
    Eckhard Podack; Fiscal Year: 2009
    ..b>Perforin-1, a pore forming protein expressed and secreted through granule exocytosis by NK and CTL, is a central player ..
  36. STRUCTURAL PROPERTIES OF MEMBRANES
    Robert Bittman; Fiscal Year: 2003
    ..with radiolabeled photoactivatable phospholipid probes, 3) to analyze interactions between Vibrio cholerae cytolysin and sphingolipids, 4) to examine the role of the trans double bond in the long chain base of ceramide in ..
  37. INTERACTIONS & CONFORMATIONS OF DNA AT HIGH PRESSURE
    ROBERT MACGREGOR; Fiscal Year: 1993
    ..of wild-type and mutant forms of EcoRI endonuclease with DNA will be studied by perturbation footprinting (PFP), i.e. quantitative footprinting as a function of pressure, temperature, and ionic strength...
  38. The Neuromechanics of Patellofemoral Pain
    Nicole Wilson; Fiscal Year: 2006
    Patellofemoral pain (PFP) syndrome is one of the most common abnormalities involving the knee...
  39. Human B cell homeostasis in a new transgenic xenochimera
    Robert Woodland; Fiscal Year: 2005
    ..We present preliminary experiments to show robust B cell engraftment in NOD-rag-/-Pfp-/- recipients of human PBL supplemented daily with recombinant human BLyS...
  40. Children of Depressed Parents: Family Based Prevention
    Guy Diamond; Fiscal Year: 2006
    ..this deficit by sup porting the development, manualization and pilot testing of the Protecting Families Program (PFP), a family-based, multi-component prevention program for low-income, urban, depressed parents and their offspring...
  41. Patellofemoral Pain: Tibiofemoral Rotation Impairments
    GRETCHEN SALSICH; Fiscal Year: 2007
    ..pain condition, the proposed studies are designed to test the general hypothesis that patellofemoral pain (PFP) develops in response to abnormal patellofemoral joint contact patterns, which presumably lead to altered joint ..
  42. Mechanics of non-contact ACL injuries in female athletes
    Christopher Powers; Fiscal Year: 2007
    ..abstract_text> ..
  43. Surface Shape Based Screening of Large Protein Databases
    Daisuke Kihara; Fiscal Year: 2009
    ..The docking algorithm will be applied to protein-protein interaction data of coli. ..
  44. Relationship Context and condom Use to Prevent STDs
    Jeanne Tschann; Fiscal Year: 2006
    ..The results will provide critical information to refine STD/HIV interventions that teach safer sex negotiation skills, and to develop culturally appropriate interventions for Latino adolescents. ..
  45. 2007 Phagocytes
    Joel Swanson; Fiscal Year: 2007
    ..abstract_text> ..
  46. Mechanisms of Anthrax Toxin Signaling in Macrophages
    Joel Swanson; Fiscal Year: 2003
    ..In doing so, they will guide the selection and design of effective, late-stage therapeutics. ..
  47. CONTROL OF EPIDEMIC INFLUENZA
    PEDRO PIEDRA; Fiscal Year: 2007
    ..abstract_text> ..
  48. Coordination of signaling during phagocytosis
    Joel A Swanson; Fiscal Year: 2010
    ..Because these studies will measure signal amplitudes as a function of particle size, they should identify size thresholds for FcR signaling and determine the relative contribution of 3'Pls to those thresholds. ..
  49. ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERY
    Natalya Y Rapoport; Fiscal Year: 2010
    ....
  50. PROTEIN QUALITY CONTROL IN THE ENDOPLASMIC RETICULUM
    KARL WITTRUP; Fiscal Year: 2001
    ....
  51. ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERY
    Natalya Rapoport; Fiscal Year: 2001
    ....
  52. A Novel Cholesterol-Dependent Cytolysin Receptor
    Rodney Tweten; Fiscal Year: 2008
    ..The discovery and study of a CDC receptor will have a significant impact on the study of CDCs and will alter our perception of their role in a large number of diseases caused by a wide variety of Gram-positive pathogens. ..
  53. Manipulation of Phagocytosis in Macrophage
    Joel Swanson; Fiscal Year: 2008
    ..Consequent effects on the efficiency of Lm escape from vacuoles will be quantified. ..
  54. Engineering and Analysis of T cell CD3 and IL2R Signals
    KARL WITTRUP; Fiscal Year: 2008
    ..These studies will provide insights into T cell regulation as well as develop CDS and IL-2 stimulatory molecules of potential therapeutic value. ..