pancreatic ribonuclease

Summary

Gene Symbol: pancreatic ribonuclease
Description: ribonuclease A family member 1, pancreatic
Alias: RAC1, RIB1, RNS1, ribonuclease pancreatic, HP-RNase, RIB-1, RNase 1, RNase A, RNase upI-1, ribonuclease 1, ribonuclease A C1, ribonuclease, RNase A family, 1 (pancreatic)
Species: human

Top Publications

  1. ncbi Degradation of double-stranded RNA by human pancreatic ribonuclease: crucial role of noncatalytic basic amino acid residues
    Salvatore Sorrentino
    Department of Biological Chemistry, University Federico II of Naples, Naples, Italy
    Biochemistry 42:10182-90. 2003
  2. ncbi Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, Barcelona, 08034, Spain
    J Mol Biol 303:49-60. 2000
  3. ncbi Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants
    D Gaur
    Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India
    J Biol Chem 276:24978-84. 2001
  4. ncbi Glycine 38 is crucial for the ribonucleolytic activity of human pancreatic ribonuclease on double-stranded RNA
    Deepak Gaur
    Immunochemistry Laboratory, National Institute of Immunology, New Delhi 110 067, India
    Biochem Biophys Res Commun 297:390-5. 2002
  5. ncbi Glycosylation of human pancreatic ribonuclease: differences between normal and tumor states
    Rosa Peracaula
    Departament de Biologia, Universitat de Girona, Campus de Montilivi s n 17071, Girona, Spain
    Glycobiology 13:227-44. 2003
  6. ncbi The amino acid sequence of human pancreatic ribonuclease
    J J Beintema
    Anal Biochem 136:48-64. 1984
  7. ncbi Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, 08034 Barcelona, Spain
    Acta Crystallogr D Biol Crystallogr 57:498-505. 2001
  8. ncbi Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1)
    Julien B P Landré
    INSERM EPI 0113, Universite Bordeaux I, Avenue des Facultes, Talence, France
    J Cell Biochem 86:540-52. 2002
  9. ncbi Killing of endothelial cells and release of arachidonic acid. Synergistic effects among hydrogen peroxide, membrane-damaging agents, cationic substances, and proteinases and their modulation by inhibitors
    I Ginsburg
    Department of Oral Biology, Hebrew University Hadassah School of Medicine, Jerusalem, Israel
    Inflammation 17:295-319. 1993
  10. pmc Molecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme
    Rosa A Vacca
    Institute of Biomembranes and Bioenergetics, CNR, Via Amendola 165 A, I 70126 Bari, Italy
    BMC Biochem 9:17. 2008

Research Grants

  1. Ronald T Raines; Fiscal Year: 2016
  2. UNFOLDING OF PANCREATIC RIBONUCLEASE AND DERIVATIVES
    FREDERICK BENZ; Fiscal Year: 1980
  3. LAUE DIFFRACTION STUDY OF BOVINE PANCREATIC RIBONUCLEASE
    Edwin Westbrook; Fiscal Year: 1993
  4. 500 MHZ NMR INSTRUMENTATION FOR RESEARCH
    HAROLD SCHERAGA; Fiscal Year: 1991
  5. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2010
  6. 5 NUCLEASE ASSAYS FOR RODENT HEALTH MONITORING
    David Besselsen; Fiscal Year: 2002
  7. PATHOGENESIS OF MURINE PARVOVIRUS FIELD STRAINS
    David Besselsen; Fiscal Year: 2007
  8. SPECTROSCOPIC/KINETIC STUDIES OF FOLDING PATHWAYS
    HAROLD SCHERAGA; Fiscal Year: 2008

Detail Information

Publications196 found, 100 shown here

  1. ncbi Degradation of double-stranded RNA by human pancreatic ribonuclease: crucial role of noncatalytic basic amino acid residues
    Salvatore Sorrentino
    Department of Biological Chemistry, University Federico II of Naples, Naples, Italy
    Biochemistry 42:10182-90. 2003
    ..Human pancreatic ribonuclease (HP-RNase) is the most powerful enzyme to degrade dsRNA within the tetrapod RNase superfamily, being 500-..
  2. ncbi Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, Barcelona, 08034, Spain
    J Mol Biol 303:49-60. 2000
    We have determined the crystal structure of a human pancreatic ribonuclease or RNase 1 variant at 1.65 A resolution. Five residues in the N-terminal region were substituted by the corresponding amino acids of the bovine seminal RNase...
  3. ncbi Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants
    D Gaur
    Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India
    J Biol Chem 276:24978-84. 2001
    ..Human pancreatic ribonuclease (HPR) is structurally and functionally very similar to bovine RNase A and interacts with human RI with a ..
  4. ncbi Glycine 38 is crucial for the ribonucleolytic activity of human pancreatic ribonuclease on double-stranded RNA
    Deepak Gaur
    Immunochemistry Laboratory, National Institute of Immunology, New Delhi 110 067, India
    Biochem Biophys Res Commun 297:390-5. 2002
    Human pancreatic ribonuclease (HPR) and bovine seminal ribonuclease (BS-RNase) exhibit significantly higher activity against double stranded RNA (dsRNA), compared to RNase A...
  5. ncbi Glycosylation of human pancreatic ribonuclease: differences between normal and tumor states
    Rosa Peracaula
    Departament de Biologia, Universitat de Girona, Campus de Montilivi s n 17071, Girona, Spain
    Glycobiology 13:227-44. 2003
    Characterization of the N-glycans from human pancreatic ribonuclease (RNase 1) isolated from healthy pancreas and from pancreatic adenocarcinoma tumor cells (Capan-1 and MDAPanc-3) revealed completely different glycosylation patterns...
  6. ncbi The amino acid sequence of human pancreatic ribonuclease
    J J Beintema
    Anal Biochem 136:48-64. 1984
    The primary structure of human (Homo sapiens) pancreatic ribonuclease has been determined by automatic sequencing of the native protein and by analysis of peptides obtained by cleavage with proteolytic enzymes, cyanogen bromide, and ..
  7. ncbi Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, 08034 Barcelona, Spain
    Acta Crystallogr D Biol Crystallogr 57:498-505. 2001
    Human pancreatic ribonuclease 1 (RNase 1) is considered to be the human counterpart of bovine pancreatic RNase A...
  8. ncbi Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1)
    Julien B P Landré
    INSERM EPI 0113, Universite Bordeaux I, Avenue des Facultes, Talence, France
    J Cell Biochem 86:540-52. 2002
    ..The selective strong release of pancreatic-type RNase by endothelial cells suggests that it is endowed with non-digestive functions and involved in vascular homeostasis...
  9. ncbi Killing of endothelial cells and release of arachidonic acid. Synergistic effects among hydrogen peroxide, membrane-damaging agents, cationic substances, and proteinases and their modulation by inhibitors
    I Ginsburg
    Department of Oral Biology, Hebrew University Hadassah School of Medicine, Jerusalem, Israel
    Inflammation 17:295-319. 1993
    ..agents: the cationic substances, nuclear histone, defensins, lysozyme, poly-L-arginine, spermine, pancreatic ribonuclease, polymyxin B, chlorhexidine, cetyltrimethyl ammonium bromide, as well as by the membrane-damaging agents ..
  10. pmc Molecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme
    Rosa A Vacca
    Institute of Biomembranes and Bioenergetics, CNR, Via Amendola 165 A, I 70126 Bari, Italy
    BMC Biochem 9:17. 2008
    ..Here we report on an attempt to re-enact the emergence of a PLP-dependent protoenzyme. The starting protein was pancreatic ribonuclease A (RNase), in which active-site Lys41 or Lys7 readily form a covalent adduct with PLP.
  11. ncbi The phylogenetic position of "Acomyinae" (Rodentia, Mammalia) as sister group of a Murinae + Gerbillinae clade: evidence from the nuclear ribonuclease gene
    J Y Dubois
    Rijks Universiteit Groningen, Nijenborgh 4, Groningen, 9747 AG, The Netherlands
    Mol Phylogenet Evol 13:181-92. 1999
    The phylogenetic relationships of Acomys and Uranomys within Muridae were investigated using nuclear pancreatic ribonuclease A gene sequences...
  12. pmc Recognition of ribonuclease A by 3'-5'-pyrophosphate-linked dinucleotide inhibitors: a molecular dynamics/continuum electrostatics analysis
    Savvas Polydoridis
    Department of Physics, University of Cyprus, Nicosia, Cyprus
    Biophys J 92:1659-72. 2007
    The proteins of the pancreatic ribonuclease A (RNase A) family catalyze the cleavage of the RNA polymer chain...
  13. ncbi Activation pathways of 5-fluorouracil in rat organs and in PC12 cells
    L Mascia
    Department of Physiology and Biochemistry, University of Pisa, Laboratory of Biochemistry, Via S Maria 55, I 56126 Pisa, Italy
    Biochem Pharmacol 62:213-8. 2001
    ..of ribose 5-phosphate to 5-FU as catalysed by orotate phosphoribosyltransferase; 2) the ribose 1-phosphate (Rib1-P)-mediated addition of ribose by uridine phosphorylase, followed by the action of uridine kinase; and 3) the 2'-..
  14. pmc Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes
    W Ardelt
    Alfacell Corporation, 300 Atrium Drive, Somerset, NJ 08873, USA
    Curr Pharm Biotechnol 9:215-25. 2008
    Rana pipiens oocytes contain two homologues of pancreatic ribonuclease A that are cytostatic and cytotoxic to human cancer cells...
  15. doi Multiple solvent crystal structures of ribonuclease A: an assessment of the method
    Michelle Dechene
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Proteins 76:861-81. 2009
    ..The crystal structures of bovine pancreatic ribonuclease A (RNAse A) soaked in the following organic solvents are presented: 50% dioxane, 50% dimethylformamide, ..
  16. ncbi Purification and characterization of a ribonuclease from human liver
    S Sorrentino
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    J Biol Chem 263:16125-31. 1988
    ..composition and the amino-terminal sequence of the enzyme indicate that the protein is related to human pancreatic ribonuclease and to angiogenin, and that it may be identical with an eosinophil-derived neurotoxin and to a ..
  17. ncbi Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease
    C Schuller
    Biochemisch Laboratorium, Rijksuniversiteit Groningen, The Netherlands
    Mol Biol Evol 7:29-44. 1990
    ..Comparison with the rat pancreatic ribonuclease sequence showed a high rate of nucleotide substitution...
  18. pmc Natural merodiploidy of the lux-rib operon of Photobacterium leiognathi from coastal waters of Honshu, Japan
    Jennifer C Ast
    Department of Ecological and Evolutionary Biology, University of Michigan, 830 North University Ave, Ann Arbor, MI 48109 1048, USA
    J Bacteriol 189:6148-58. 2007
    ..13.1 defined two complete, physically separate, and apparently functional operons, designated lux-rib1 and lux-rib2. P. leiognathi strains lelon.2.1 and lnuch.21...
  19. ncbi Tandemization endows bovine pancreatic ribonuclease with cytotoxic activity
    Franziska Leich
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Str 3, 06120 Halle, Germany
    J Mol Biol 358:1305-13. 2006
    Due to their ability to degrade RNA, selected members of the bovine pancreatic ribonuclease A (RNase A) superfamily are potent cytotoxins...
  20. doi Modeling formalin fixation and histological processing with ribonuclease A: effects of ethanol dehydration on reversal of formaldehyde cross-links
    Carol B Fowler
    Department of Biophysics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 88:785-91. 2008
    ..Our initial studies of single proteins, such as bovine pancreatic ribonuclease A (RNase A), in 10% buffered formalin solution revealed that upon removal of excess formaldehyde, ..
  21. doi Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production
    Kostyantyn V Dmytruk
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Metab Eng 13:82-8. 2011
    ..factor SEF1 and IMH3 (coding for IMP dehydrogenase) orthologs from Debaryomyces hansenii, and the homologous genes RIB1 and RIB7, encoding GTP cyclohydrolase II and riboflavin synthetase, the first and the last enzymes of riboflavin ..
  22. doi Bactericidal activity engineered on human pancreatic ribonuclease and onconase
    Gerard Torrent
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi s n 17071 Girona, Spain
    Mol Pharm 6:531-42. 2009
    ..In the present paper, however, we have used two nonbactericidal ribonucleases, onconase and the human pancreatic ribonuclease, as scaffolds onto which to engineer bactericidal activity...
  23. pmc Trifluoroethanol-induced conformational transitions of proteins: insights gained from the differences between alpha-lactalbumin and ribonuclease A
    K Gast
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Protein Sci 8:625-34. 1999
    ..changes of two proteins widely used in folding experiments, bovine alpha-lactalbumin, and bovine pancreatic ribonuclease A, have been investigated...
  24. pmc Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity
    Thomas J Rutkoski
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Curr Pharm Biotechnol 9:185-9. 2008
    Onconase (ONC) is an amphibian member of the bovine pancreatic ribonuclease (RNase A) superfamily that exhibits innate antitumoral activity. ONC has been granted both orphan-drug and fast-track status by the U.S...
  25. doi Adaptive evolution of digestive RNASE1 genes in leaf-eating monkeys revisited: new insights from ten additional colobines
    Li Yu
    Laboratory for Conservation and Utilization of Bio resource and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China
    Mol Biol Evol 27:121-31. 2010
    ....
  26. ncbi Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution
    D D Leonidas
    School of Biology and Biochemistry, University of Bath, Claverton Down, U K
    Biochemistry 36:5578-88. 1997
    High-resolution (1.7 A) crystal structures have been determined for bovine pancreatic ribonuclease A (RNase A) complexed with 5'-diphosphoadenosine 3'-phosphate (ppA-3'-p) and 5'-diphosphoadenosine 2'-phosphate (ppA-2'-p), as well as for ..
  27. ncbi The crystal structure of eosinophil cationic protein in complex with 2',5'-ADP at 2.0 A resolution reveals the details of the ribonucleolytic active site
    C Gopi Mohan
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U K
    Biochemistry 41:12100-6. 2002
    ..ECP belongs to the pancreatic ribonuclease superfamily of proteins, and the crystal structure of ECP in the unliganded form (determined previously) ..
  28. ncbi Production of human pancreatic ribonuclease in Saccharomyces cerevisiae and Escherichia coli
    M Ribo
    Unitat de Bioquímica i Biologia Molecular, Departament de Biologia, Universitat de Girona, Girona, Spain
    Protein Expr Purif 7:253-61. 1996
    Human pancreatic ribonuclease (HP-RNase) has considerable promise as a therapeutic agent. Structure-function analyses of HP-RNase have been impeded by the difficulty of obtaining the enzyme from its host...
  29. doi Toward an antitumor form of bovine pancreatic ribonuclease: the crystal structure of three noncovalent dimeric mutants
    Antonello Merlino
    Department of Chemistry, University of Naples, Complesso Universitario Monte S Angelo, Via Cinthia, I 80126 Naples, Italy
    Biopolymers 91:1029-37. 2009
    ..The introduction of Leu28, Cys31, and Cys32 and, in addition, of Pro19 in the sequence of bovine pancreatic ribonuclease (RNase A) has produced two dimeric variants LCC and PLCC, which do exhibit a cytotoxic activity, though ..
  30. ncbi The structural determinants that lead to the formation of particular oligomeric structures in the pancreatic-type ribonuclease family
    A Benito
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi s n, E 17071, Girona, Spain
    Curr Protein Pept Sci 9:370-93. 2008
    ..The prototype of this family is bovine pancreatic ribonuclease or ribonuclease A...
  31. ncbi Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats
    B Kobe
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9050
    Nature 366:751-6. 1993
    Ribonuclease inhibitor is a cytoplasmic protein that tightly binds and inhibits ribonucleases of the pancreatic ribonuclease superfamily...
  32. ncbi Cytotoxicity of bovine seminal ribonuclease: monomer versus dimer
    J Eugene Lee
    Departments of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Biochemistry 44:15760-7. 2005
    Bovine seminal ribonuclease (BS-RNase) is a homologue of bovine pancreatic ribonuclease (RNase A). Unlike RNase A, BS-RNase has notable toxicity for human tumor cells...
  33. pmc Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy
    Yong Bin Yan
    Department of Biological Sciences and Biotechnology, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Biophys J 90:2525-33. 2006
    ..We studied the specific events of bovine pancreatic ribonuclease A thermal aggregation by a combination of second derivative infrared analysis and two-dimensional ..
  34. ncbi A new mutant of bovine seminal ribonuclease with a reversed swapping propensity
    Carmine Ercole
    Dipartimento di Chimica, Universita Federico II di Napoli, Via Cintia, 80126 Napoli, Italy
    Biochemistry 46:2227-32. 2007
    ..mutants by replacing selected residues with the corresponding ones of its monomeric counterpart, bovine pancreatic ribonuclease (RNase A)...
  35. pmc 'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry: crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines
    Hidenori Yamada
    Department of Medical and Bioengineering Science, Graduate School of Natural Science and Technology, Okayama University, Japan
    Protein Sci 16:1389-97. 2007
    ..interface (comprising four leucine residues) was introduced into a helical region (helix 2) of the human pancreatic ribonuclease 1 (RNase 1) that was predicted to form a suitable crystallization interface...
  36. pmc Conformational changes below the Tm: molecular dynamics studies of the thermal pretransition of ribonuclease A
    Eric D Merkley
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 5061, USA
    Biochemistry 47:880-92. 2008
    ..variation, we have performed all-atom, explicit-solvent molecular dynamics simulations of bovine pancreatic ribonuclease A (RNase A) up to its melting temperature (Tm approximately 337 K)...
  37. pmc Dissimilarity in the oxidative folding of onconase and ribonuclease A, two structural homologues
    Robert F Gahl
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Protein Eng Des Sel 21:223-31. 2008
    ..A family, was examined and shows markedly different behavior compared to its structural homologue bovine pancreatic ribonuclease A (RNase A) under similar conditions...
  38. doi The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS
    Veronique Blanchard
    Bijvoet Center, Department of Bio Organic Chemistry, Utrecht University, Padualaan 8, NL 3584 CH Utrecht, The Netherlands
    Biochemistry 47:3435-46. 2008
    ..A typical example is native bovine pancreatic ribonuclease B (RNase B) with oligomannose-type N-glycans at Asn-34...
  39. pmc Embryo transfer rederivation of C.B-17/Icr-Prkdc(scid) mice experimentally infected with mouse parvovirus 1
    David G Besselsen
    Department of University Animal Care, University of Arizona, Tucson, AZ, USA
    Comp Med 58:353-9. 2008
    ..All of these mice were negative by MFI for parvovirus nonstructural protein antigen (rNS1) and by qPCR, with the exception of 1 recipient female mouse that displayed weak rNS1 seroreactivity and low levels ..
  40. pmc Structural features for the mechanism of antitumor action of a dimeric human pancreatic ribonuclease variant
    Antonello Merlino
    Dipartimento di Chimica, Universita degli Studi di Napoli Federico II, Via Cintia, Napoli 80126, Italy
    Protein Sci 18:50-7. 2009
    ....
  41. pmc Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleolytic activities of angiogenin
    R Shapiro
    Proc Natl Acad Sci U S A 84:2238-41. 1987
    ..Assays measuring competition between angiogenin and bovine pancreatic ribonuclease A for PRI reveal that binding of the inhibitor to angiogenin is extremely tight, with a Ki value well ..
  42. pmc Swapping structural determinants of ribonucleases: an energetic analysis of the hinge peptide 16-22
    L Mazzarella
    Centro di Studio di Biocristallografia, Universita di Napoli Federico II, Naples, Italy
    Proc Natl Acad Sci U S A 92:3799-803. 1995
    Bovine seminal ribonuclease (BS-RNase) is a homodimeric enzyme strictly homologous to the pancreatic ribonuclease (RNase A)...
  43. ncbi The Arabidopsis ribonuclease gene RNS1 is tightly controlled in response to phosphate limitation
    P A Bariola
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824 1312
    Plant J 6:673-85. 1994
    ..Here, the control of two closely related ribonuclease genes of Arabidopsis, RNS1 and RNS3 is investigated...
  44. ncbi Detection of antibodies against porcine parvovirus nonstructural protein NS1 may distinguish between vaccinated and infected pigs
    E S Madsen
    Danish Veterinary Institute for Virus Research, Kalvehave, Denmark
    Vet Microbiol 54:1-16. 1997
    ..Sf9 cells (Spodoptora frugidiperda) inoculated with AcNPV-NS1 producing recombinant nonstructural protein (rNS1) and AcNPV-VP2 producing recombinant virion protein (rVP2) were used in IPT and ELISA to analyse serum antibodies...
  45. ncbi Conformational propensities of protein folding intermediates: distribution of species in the 1S, 2S, and 3S ensembles of the [C40A,C95A] mutant of bovine pancreatic ribonuclease A
    William J Wedemeyer
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 41:1483-91. 2002
    ..We address this problem by studying the conformational propensities of reductively unfolded RNase A under folding conditions, since earlier work has indicated that the equilibrium conformational ensemble of fully ..
  46. ncbi Secondary structure analysis of proteins embedded in spherical polyelectrolyte brushes by FT-IR spectroscopy
    Alexander Wittemann
    Physikalische Chemie I, Universitat Bayreuth, Universitaetsstrasse 30, D 95440 Bayreuth, Germany
    Anal Chem 76:2813-9. 2004
    The adsorption of bovine serum albumin (BSA), bovine beta-lactoglobulin, and bovine pancreatic ribonuclease A onto spherical polyelectrolyte brushes (SPB) is reported...
  47. ncbi Insertional-fusion of basic fibroblast growth factor endowed ribonuclease 1 with enhanced cytotoxicity by steric blockade of inhibitor interaction
    Hiroko Tada
    Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    FEBS Lett 568:39-43. 2004
    Basic fibroblast growth factor (bFGF) was inserted in the middle of human ribonuclease 1 (RNase1) sequence at an RNase inhibitor (RI)-binding site (Gly89) by a new gene fusion technique, insertional-fusion...
  48. ncbi Use of isotopic signatures for mass spectral detection of protein adduction by chemically reactive metabolites of bromobenzene: studies with model proteins
    Weimin Yue
    Department of Medicinal Chemistry, University of Kansas, Malott Hall, Lawrence, Kansas 66045, USA
    Chem Res Toxicol 18:1748-54. 2005
    ..1) and Nalpha-acetyl-Nepsilon-(p-bromophenyl)-L-lysine (2) to bovine pancreatic ribonuclease A...
  49. ncbi A ternary nucleation model for the nucleation pathway of protein folding
    Y S Djikaev
    Department of Chemical and Biological Engineering, SUNY at Buffalo, Buffalo, New York 14260, USA
    J Chem Phys 126:175103. 2007
    ..As an illustration, the model is applied to the folding of bovine pancreatic ribonuclease consisting of 124 amino acids whereof 40 are hydrophobic, 81 hydrophilic, and 3 neutral...
  50. pmc Tuning the pK(a) of fluorescein to optimize binding assays
    Luke D Lavis
    Departments of Chemistry and Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Anal Chem 79:6775-82. 2007
    ..The fluorescence of fluorescein-labeled model protein, bovine pancreatic ribonuclease (RNase A), decreases upon binding to its cognate inhibitor protein (RI)...
  51. ncbi Characterization of human angiogenin variants implicated in amyotrophic lateral sclerosis
    Benedict Crabtree
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom
    Biochemistry 46:11810-8. 2007
    Human angiogenin (ANG), the first member of the angiogenin family (from the pancreatic ribonuclease A superfamily) to be identified, is an angiogenic factor that induces neovascularization...
  52. doi The molecular characterization of a novel GH38 α-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins
    Beatrice Cobucci-Ponzano
    Institute of Protein Biochemistry Consiglio Nazionale delle Ricerche, Via P Castellino 111, 80131 Naples, Italy
    Biochimie 92:1895-907. 2010
    ..More interestingly, Ssα-man removes mannose residues from the glycosidic moiety of the bovine pancreatic ribonuclease B, suggesting that it could process mannosylated proteins also in vivo...
  53. pmc Antitumor activity of ribonuclease multimers created by site-specific covalent tethering
    Thomas J Rutkoski
    Departments of Biochemistry and Chemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Bioconjug Chem 21:1691-702. 2010
    ..A monomeric RI-evasive variant of bovine pancreatic ribonuclease (RNase A) inhibits the growth of human prostate and lung tumors in mice...
  54. pmc A human ribonuclease induces apoptosis associated with p21WAF1/CIP1 induction and JNK inactivation
    Jessica Castro
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi s n E 17071 Girona, Spain
    BMC Cancer 11:9. 2011
    ..The mechanism of cytotoxicity of PE5, a variant of human pancreatic ribonuclease carrying a nuclear localization signal, has been investigated and compared to that of onconase.
  55. pmc Bovine pancreatic ribonuclease: fifty years of the first enzymatic reaction mechanism
    Claudi M Cuchillo
    Departament de Bioquímica i Biologia Molecular, Unitat de Biociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
    Biochemistry 50:7835-41. 2011
    ..The Mathias and Rabin mechanism has appeared in numerous textbooks, monographs, and reviews and continues to have a profound impact on biochemistry...
  56. pmc PHF6 regulates cell cycle progression by suppressing ribosomal RNA synthesis
    Jiadong Wang
    Department of Experimental Radiation Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 288:3174-83. 2013
    ..Together, these results reveal that the key function of PHF6 is involved in regulating rRNA synthesis, which may contribute to its roles in cell cycle control, genomic maintenance, and tumor suppression...
  57. pmc MicroRNA drop in the bloodstream and microRNA boost in the tumour caused by treatment with ribonuclease A leads to an attenuation of tumour malignancy
    Nadezhda Mironova
    Laboratory of Nucleic Acids Biochemistry, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russian Federation
    PLoS ONE 8:e83482. 2013
    ..Recently, antitumour and antimetastatic activities of pancreatic ribonuclease A were demonstrated and here genome-wide profiles of microRNAs in the tumour and blood of mice bearing ..
  58. doi Mussel-inspired new approach for polymerization and cross-linking of peptides and proteins containing tyrosines by Fremy's salt oxidation
    Meir Wilchek
    Department of Biological Chemistry, Wiezmann Institue of Science, Rehovot 76100, Israel
    Bioconjug Chem 26:502-10. 2015
    ..The proteins tested were as follows: bovine pancreatic ribonuclease A (RNase), lysozyme, IgG, avidin, and streptavidin...
  59. pmc Mia40 is a facile oxidant of unfolded reduced proteins but shows minimal isomerase activity
    Devin A Hudson
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States
    Arch Biochem Biophys 579:1-7. 2015
    ..Reduced pancreatic ribonuclease A (rRNase), avian lysozyme, and riboflavin binding protein are all competent substrates of the Mia40/..
  60. doi Interactions of carboplatin and oxaliplatin with proteins: Insights from X-ray structures and mass spectrometry studies of their ribonuclease A adducts
    Luigi Messori
    Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy Electronic address
    J Inorg Biochem 153:136-42. 2015
    ..Here, the X-ray structures of the adducts formed upon reaction of carboplatin and oxaliplatin with bovine pancreatic ribonuclease (RNase A) are reported and compared with results obtained for the structure of the RNase A-cisplatin ..
  61. pmc Changes in brain ribonuclease (BRB) messenger RNA in granulosa cells (GCs) of dominant vs subordinate ovarian follicles of cattle and the regulation of BRB gene expression in bovine GCs
    J L Dentis
    Department of Animal Science, Oklahoma State University, Stillwater, OK, 74078, USA
    Domest Anim Endocrinol 55:32-40. 2016
    ..A superfamily that is constitutively expressed in a range of tissues and is the functional homolog of human ribonuclease 1. This study was designed to characterize BRB gene expression in granulosa cells (GCs) during development of ..
  62. pmc Expression Patterns and Functional Novelty of Ribonuclease 1 in Herbivorous Megalobrama amblycephala
    Han Liu
    Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
    Int J Mol Sci 17:. 2016
    b>Ribonuclease 1 (RNase1) is an important digestive enzyme that has been used to study the molecular evolutionary and plant-feeding adaptation of mammals...
  63. ncbi Assignment of pancreatic ribonuclease gene to mouse chromosome 14
    R W Elliott
    Cytogenet Cell Genet 42:110-2. 1986
    A pancreatic ribonuclease cDNA was used as a probe for Southern blot hybridization of genomic DNA from recombinant inbred strains of mice...
  64. ncbi Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11
    S B delCardayre
    Department of Biochemistry, University of Wisconsin Madison 53706 1569, USA
    Protein Eng 8:261-73. 1995
    Bovine pancreatic ribonuclease A (RNase A) has been the object of much landmark work in biological chemistry...
  65. ncbi Structural features that determine the enzymatic potency and specificity of human angiogenin: threonine-80 and residues 58-70 and 116-123
    R Shapiro
    Center for Biochemical and Biophysical Sciences and Medicine, Boston, Massachusetts 02115, USA
    Biochemistry 37:6847-56. 1998
    Human angiogenin (Ang), a homologue of bovine pancreatic ribonuclease A (RNase A), is a potent inducer of blood vessel formation...
  66. pmc Effects of sucrose on conformational equilibria and fluctuations within the native-state ensemble of proteins
    Yong Sung Kim
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Protein Sci 12:1252-61. 2003
    ..model osmolyte, on conformational equilibria and fluctuations within the native-state ensembles of bovine pancreatic ribonuclease A and S and horse heart cytochrome c...
  67. pmc Minor folding defects trigger local modification of glycoproteins by the ER folding sensor GT
    Christiane Ritter
    Institute of Biochemistry, Swiss Federal Institute of Technology, Zurich, Switzerland
    EMBO J 24:1730-8. 2005
    ..Using an improved inhibitor assay based on soluble conformers of pancreatic ribonuclease in its glycosylated (RNase B) and unglycosylated (RNase A) forms, we found that the protein moiety of a ..
  68. pmc The rib1 mutant of Arabidopsis has alterations in indole-3-butyric acid transport, hypocotyl elongation, and root architecture
    Julie Poupart
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Plant Physiol 139:1460-71. 2005
    ..the first demonstration of altered IBA transport in an Arabidopsis mutant, and show that the resistant to IBA (rib1) mutation results in alterations in growth, development, and response to exogenous auxin consistent with an ..
  69. ncbi Two-metal-ion mechanism for hammerhead-ribozyme catalysis
    Fabrice Leclerc
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, Universite Henri Poincare, Faculte des Sciences, 54506 Vandoeuvre les Nancy, France
    J Phys Chem B 110:3395-409. 2006
    ..Thus, the overall reaction corresponds to that catalyzed by bovine pancreatic ribonuclease. However, an essential distinguishing aspect is that metal ions are not involved in RNase catalysis but ..
  70. doi Electric charge divergence in proteins: insights into the evolution of their three-dimensional properties
    Yukuto Sato
    Division of Molecular Marine Biology, Ocean Research Institute, University of Tokyo, Minamidai 1 15 1, Nakano Ku, Tokyo 164 8639, Japan
    Gene 441:3-11. 2009
    ..To obtain further insights, ALD and PGI were compared to the proteins pancreatic ribonuclease (EC 3.1.27.5) and triose-phosphate isomerase (EC 5.3.1...
  71. pmc FAMBE-pH: a fast and accurate method to compute the total solvation free energies of proteins
    Yury N Vorobjev
    Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Science, Novosibirsk, Russia
    J Phys Chem B 112:11122-36. 2008
    ..proteins, namely, bovine pancreatic trypsin inhibitor (BPTI), hen egg white lysozyme (HEWL), and bovine pancreatic ribonuclease A (RNaseA)...
  72. doi Super induction of dengue virus NS1 protein in E. coli
    Dipankar Das
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304 89 Avenue, Edmonton, Alberta, Canada
    Protein Expr Purif 66:66-72. 2009
    ..The NS1 protein was successfully affinity purified and refolded as a recombinant NS1 (rNS1) protein in E. coli and yield was 230-250 mg/L of bacterial culture...
  73. doi Terrimonas aquatica sp. nov., isolated from a freshwater spring
    Shih Yi Sheu
    Department of Marine Biotechnology, National Kaohsiung Marine University, No 142, Hai Chuan Rd, Nan Tzu, Kaohsiung City 811, Taiwan, ROC
    Int J Syst Evol Microbiol 60:2705-9. 2010
    A yellow-pigmented bacterial strain, designated RIB1-6(T), was isolated from a freshwater spring in Taiwan. Strain RIB1-6(T) was aerobic, Gram-negative, rod-shaped, non-motile and non-spore-forming...
  74. doi Protein side-chain translocation mutagenesis via incorporation of peptoid residues
    Byoung Chul Lee
    Biological Nanostructures Facility, The Molecular Foundry, Lawrence Berkeley National Laboratory, California 94720, USA
    ACS Chem Biol 6:1367-74. 2011
    ..were able to introduce peptoid residues (N-substituted glycine residues) at defined positions into bovine pancreatic ribonuclease A...
  75. doi Chain termini cross-talk in the swapping process of bovine pancreatic ribonuclease
    Antonello Merlino
    Department of Chemistry, University of Naples Federico II, Complesso Universitario Monte S Angelo, Via Cinthia, I 80126 Naples, Italy
    Biochimie 94:1108-18. 2012
    ..Bovine pancreatic ribonuclease (RNase A) is able to swap the N-terminal α-helix (residues 1-13) and/or the C-terminal β-strand (..
  76. pmc Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle
    Ornnuthchar Poungpair
    Division of Molecular Medicine Department of Research and Development Faculty of Medicine Siriraj Hospital Mahidol University Bangkok, Thailand
    MAbs 6:474-82. 2014
    ..Recombinant NS1 (rNS1) of DENV serotype 2 (DENV2) was used as antigen in phage bio-panning to select phage clones that displayed HuScFv ..
  77. doi Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability
    Delia Picone
    Department of Chemical Sciences, University of Naples Federico II, Via Cintia, I 80126 Naples, Italy Electronic address
    J Inorg Biochem 146:37-43. 2015
    ..reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and ..
  78. doi Acclimation of the crucifer Eutrema salsugineum to phosphate limitation is associated with constitutively high expression of phosphate-starvation genes
    Vera Marjorie Elauria Velasco
    Department of Biology, McMaster University, Hamilton, ON, L8S 4K1, Canada
    Plant Cell Environ 39:1818-34. 2016
    ..Absolute quantification of transcripts indicated that PHR1, WRKY75 and RNS1 were expressed at higher levels in Eutrema plants relative to those in Arabidopsis regardless of external Pi...
  79. doi Live cell fluorescence imaging for early expression and localization of RIB1 and RIB3 genes in Ashbya gossypii
    Sudeshna Sengupta
    Department of Biotechnology, Indian Institute of Technology, Chennai, India
    J Basic Microbiol 54:81-7. 2014
    Ashbya gossypii is a riboflavin overproducing filamentous fungus. RIB1 and RIB3 genes encode GTP-cyclohydrolase II (GCH II) and DHBP synthase, respectively, the two rate limiting enzymes of the riboflavin biosynthetic pathway...
  80. ncbi Intrachain disulfide bridges of bovine seminal ribonuclease
    A Di Donato
    Biochim Biophys Acta 579:303-13. 1979
    ..identical with those of the eight half-cystine residues of the strictly homologous chain of bovine pancreatic ribonuclease. The results reported show that these eight 'homologous' half-cystine residues pair in seminal ..
  81. ncbi The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases
    J A Lenstra
    Eur J Biochem 98:399-408. 1979
    The complete amino acid sequence of mouse pancreatic ribonuclease has been determined by analysis of tryptic, chymotryptic, thermolytic and CNBr peptides and by automatic sequence analysis of the intact protein...
  82. ncbi Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme
    R J MacDonald
    J Biol Chem 257:14582-5. 1982
    We have cloned via recombinant DNA technology the mRNA sequence of rat pancreatic ribonuclease, and have determined the entire nucleotide sequence of the mature message...
  83. ncbi Atomic resolution structures of ribonuclease A at six pH values
    R Berisio
    Centro di Studio di Biocristallografia, CNR, Via Mezzocannone 6, I 80134 Napoli, Italy
    Acta Crystallogr D Biol Crystallogr 58:441-50. 2002
    ..Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1...
  84. ncbi Oxidative folding of bovine pancreatic ribonuclease A: insight into the overall catalysis of the refolding pathway by phosphate
    Lawrence K Low
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    J Protein Chem 21:19-27. 2002
    The effects of the strong stabilizing anion, phosphate, on the oxidative folding of bovine pancreatic ribonuclease A were examined. Phosphate was found to catalyze several steps involved in the oxidative folding process at pH 8...
  85. ncbi Temperature dependence of the backbone dynamics of ribonuclease A in the ground state and bound to the inhibitor 5'-phosphothymidine (3'-5')pyrophosphate adenosine 3'-phosphate
    Evgenii L Kovrigin
    Department of Chemistry, Yale University, P O Box 208107, New Haven, Connecticut 06520, USA
    Biochemistry 42:5279-91. 2003
    ..inhibitor 5'-phosphothymidine(3',5')pyrophosphate adenosine 3'-phosphate (pTppAp) with bovine pancreatic ribonuclease A (RNase A) was characterized by calorimetry and solution NMR spectroscopy...
  86. ncbi The structural and functional studies of His119 and His12 in RNase A via chemical modification
    Shahrokh Safarian
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    J Protein Chem 22:643-54. 2003
    The histidyl residues of bovine pancreatic ribonuclease A (RNase A) play a crucial role in enzymatic activity...
  87. pmc Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A: I-structural and functional alterations
    Vladimir K Rait
    Division of Biophysics, Department of Cellular Pathology and Genetics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 84:292-9. 2004
    ..We used biophysical techniques to investigate the effects of formaldehyde treatment on bovine pancreatic ribonuclease A (RNase A)...
  88. ncbi Candida famata (Debaryomyces hansenii) DNA sequences containing genes involved in riboflavin synthesis
    Andriy Y Voronovsky
    Institute of Cell Biology NASU, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Yeast 21:1307-16. 2004
    Previously cloned Candida famata (Debaryomyces hansenii) strain VKM Y-9 genomic DNA fragments containing genes RIB1 (codes for GTP cyclohydrolase II), RIB2 (encodes specific reductase), RIB5 (codes for dimethylribityllumazine synthase), ..
  89. pmc Open interface and large quaternary structure movements in 3D domain swapped proteins: insights from molecular dynamics simulations of the C-terminal swapped dimer of ribonuclease A
    Antonello Merlino
    Centro Interdipartimentale Ricerca e Management, Complesso Ristrutturato S Andrea delle Dame, 80138, Naples, Italy
    Biophys J 88:2003-12. 2005
    Bovine pancreatic ribonuclease (RNase A) forms two three-dimensional (3D) domain swapped dimers...
  90. ncbi [Conjugates of pancreatic ribonuclease and ligand-free human serum albumin]
    E A Zelepuga
    Biomed Khim 49:588-96. 2003
    ..A single dose administration of LFHSA-RNAse conjugates exhibited high antiviral activity in mice, infected with influenza A and influenza B viruses...
  91. ncbi Disruption of shape-complementarity markers to create cytotoxic variants of ribonuclease A
    Thomas J Rutkoski
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 354:41-54. 2005
    Onconase (ONC), an amphibian member of the bovine pancreatic ribonuclease A (RNase A) superfamily, is in phase III clinical trials as a treatment for malignant mesothelioma...
  92. ncbi Role of aspartic acid 121 in human pancreatic ribonuclease catalysis
    Deepak Gaur
    Immunochemistry Laboratory, National Institute of Immunology, New Delhi, India
    Mol Cell Biochem 275:95-101. 2005
    Bovine pancreatic ribonuclease (RNase A) is one of the most well studied enzymes of the ribonuclease family, unlike its human counterpart, the human pancreatic ribonuclease (HPR), whose physiological role in the body is not clearly ..
  93. ncbi Enhanced immune response by amphotericin B following NS1 protein prime-oral recombinant Salmonella vaccine boost vaccination protects mice from dengue virus challenge
    Wen Tssann Liu
    Institute of Preventive Medicine, National Defense Medical Center, P O Box 90048 505, Neihu, Taipei, Taiwan
    Vaccine 24:5852-61. 2006
    ..strain expressing a secreted dengue virus type 2 non-structural NS1 and Yersinia pestis F1 (Caf1) fusion protein, rNS1:Caf1, was generated. Immunological evaluation was performed by prime-boost vaccine regimen...
  94. doi Extracellular RNA liberates tumor necrosis factor-α to promote tumor cell trafficking and progression
    Silvia Fischer
    Department of Biochemistry, Medical School, Justus Liebig University, Giessen, Germany
    Cancer Res 73:5080-9. 2013
    ..Our findings establish a crucial role for eRNA in driving tumor progression, and they suggest applications for extracellular RNase1 as an antiinvasive regimen for cancer treatment...
  95. ncbi Prediction of recurrence in low and intermediate risk non-muscle invasive bladder cancer by real-time quantitative PCR analysis: cDNA microarray results
    J Mares
    Charles University, Prague, Czech Republic
    Neoplasma 60:295-301. 2013
    ..Our results indicate that it may be possible to identify patients with a low and high risk of disease recurrence at an early stage using a molecular profile...
  96. pmc Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues
    H F Rosenberg
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 23:4290-5. 1995
    ..encoding human ribonuclease 4 (RNase 4) of the mammalian ribonuclease gene family, whose members include pancreatic ribonuclease, eosinophil-derived neurotoxin, eosinophil cationic protein and angiogenin...
  97. ncbi The mouse angiogenin gene family: structures of an angiogenin-related protein gene and two pseudogenes
    W E Brown
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genomics 29:200-6. 1995
    Angiogenin, a homologue of pancreatic ribonuclease, is a potent inducer of blood vessel formation...
  98. ncbi Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values
    O Lequin
    Département de Chimie Synthèse Organique, URA 1308 du CNRS, Ecole Polytechnique, Palaiseau, France
    Eur J Biochem 250:712-26. 1997
    Human angiogenin is a member of the pancreatic ribonuclease superfamily that induces blood vessel formation. Its three-dimensional solution structure has been determined to high resolution by heteronuclear NMR spectroscopy...
  99. ncbi Ribonuclease inhibitor protein of human erythrocytes: characterization, loss of activity in response to oxidative stress, and association with Heinz bodies
    M Moenner
    INSERM Unité 427, Développement Humain, Croissance et Différenciation, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, France
    Blood Cells Mol Dis 24:149-64. 1998
    ..Each inhibitor forms an equimolar complex with bovine pancreatic ribonuclease A and is inactivated by treatment with the sulfhydryl reagent p-(hydroxymercuri)benzoate...
  100. ncbi Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease A on bovine oocyte maturation
    T Slavik
    Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Czech Republic
    J Exp Zool 287:394-9. 2000
    ..The MxM form and RNase A at 5 microg/ml inhibited the maturation rate by 71 and 48%, respectively, but a less significant effect was ..

Research Grants8

  1. Ronald T Raines; Fiscal Year: 2016
    Onconase (ONC), which is an amphibian homologue of bovine pancreatic ribonuclease (RNase A), is now undergoing a human clinical trial for the treatment of cancer...
  2. UNFOLDING OF PANCREATIC RIBONUCLEASE AND DERIVATIVES
    FREDERICK BENZ; Fiscal Year: 1980
    This proposal deals with the application of nmr to study the unfolding and refolding of bovine pancreatic ribonuclease (RNase) and derivatives in an attempt to detect intermediate states...
  3. LAUE DIFFRACTION STUDY OF BOVINE PANCREATIC RIBONUCLEASE
    Edwin Westbrook; Fiscal Year: 1993
    ..We will study the reaction catalyzed by bovine pancreatic ribonuclease (Rnase A) in the crystalline state as a test system because it is a standard enzyme that has been studied ..
  4. 500 MHZ NMR INSTRUMENTATION FOR RESEARCH
    HAROLD SCHERAGA; Fiscal Year: 1991
    ..H.A. Scheraga is studying the structure, function, dynamics and folding of bovine pancreatic ribonuclease A, bovine thrombin, hirudin and epidermal growth factor to better understand their 3D structure, ..
  5. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2010
    ..These studies will also help understand human smell and taste variations and disorders. ..
  6. 5 NUCLEASE ASSAYS FOR RODENT HEALTH MONITORING
    David Besselsen; Fiscal Year: 2002
    ..FFPNA will greatly enhance the diagnostic capabilities for routine health monitoring of laboratory rodents and will provide a viable, cost-effective alternative for RAP testing. ..
  7. PATHOGENESIS OF MURINE PARVOVIRUS FIELD STRAINS
    David Besselsen; Fiscal Year: 2007
    ..In addition, these studies will provide information about perturbations in host physiology and gene expression induced by murine parvoviruses, and basic scientific information about parvovirus host interaction. ..
  8. SPECTROSCOPIC/KINETIC STUDIES OF FOLDING PATHWAYS
    HAROLD SCHERAGA; Fiscal Year: 2008
    ..g., the undesirable association of sickle-cell hemogloblin, or the induction of an oncogene product, whose properties involve a conformational change when only one amino acid residue in the sequence is changed. [unreadable] [unreadable]..