p65

Summary

Gene Symbol: p65
Description: RELA proto-oncogene, NF-kB subunit
Alias: NFKB3, p65, transcription factor p65, NF-kappa-B p65delta3, NF-kappa-B transcription factor p65, nuclear factor NF-kappa-B p65 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, v-rel avian reticuloendotheliosis viral oncogene homolog A, v-rel reticuloendotheliosis viral oncogene homolog A
Species: human

Top Publications

  1. ncbi IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain
    H Sakurai
    Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, 16 89 Kashima 3 chome, Yodogawa ku, Osaka 532 8505, Japan
    J Biol Chem 274:30353-6. 1999
  2. ncbi The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes
    Lakshmanan Ganesh
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nature 426:853-7. 2003
  3. ncbi Regulation of the syncytin-1 promoter in human astrocytes by multiple sclerosis-related cytokines
    Giuseppe Mameli
    Section of Microbiology, Department of Biomedical Sciences, Center of Excellence for Biotechnology Development and Biodiversity Research, Sassari, Italy
    Virology 362:120-30. 2007
  4. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
  5. doi Curcumin inhibits hepatitis C virus replication via suppressing the Akt-SREBP-1 pathway
    Kyeongjin Kim
    Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan, Republic of Korea
    FEBS Lett 584:707-12. 2010
  6. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
  7. pmc Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression
    Satish L Deshmane
    Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania 19122, USA
    J Biol Chem 284:11364-73. 2009
  8. pmc Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes
    Xueqing Ba
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 285:11596-606. 2010
  9. pmc The cytoplasmic domain of the HIV-1 glycoprotein gp41 induces NF-κB activation through TGF-β-activated kinase 1
    Thomas S Postler
    New England Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical School, Southborough, MA 01772 9102, USA
    Cell Host Microbe 11:181-93. 2012
  10. ncbi HIV-1 Vpr: a closer look at the multifunctional protein from the structural perspective
    RAMESH C PANDEY
    Department of Biotechnology, Indian Institute of Technology, Chennai, India
    Curr HIV Res 7:114-28. 2009

Research Grants

  1. NF-kappaB Regulation by Androgen Receptor
    ALBERT BALDWIN JR; Fiscal Year: 2004
  2. Use p105(sr) to study p50 homodimer in skin tumor/cancer
    Li Lin; Fiscal Year: 2009
  3. Hypothermia enhances inflammatory cytokine expression via NF-kappa B
    Karen D Fairchild; Fiscal Year: 2010
  4. Control of NF-kappaB Activity
    ALBERT SIDNEY BALDWIN; Fiscal Year: 2013
  5. Ezra Burstein; Fiscal Year: 2015
  6. Gourisankar Ghosh; Fiscal Year: 2016
  7. Inflammatory Cytokines Link NF-kappaB with Bim Degradation and Metastasis
    Marty W Mayo; Fiscal Year: 2012
  8. Lin Feng Chen; Fiscal Year: 2014
  9. CHARLES EMORY MCCALL; Fiscal Year: 2014
  10. Lung-Liver Axis During Pneumonia
    Lee Quinton; Fiscal Year: 2012

Detail Information

Publications344 found, 100 shown here

  1. ncbi IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain
    H Sakurai
    Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, 16 89 Kashima 3 chome, Yodogawa ku, Osaka 532 8505, Japan
    J Biol Chem 274:30353-6. 1999
    ..We have examined the possibility that IKK can phosphorylate the p65 NF-kappaB subunit as well as IkappaB in the cytokine-induced NF-kappaB activation...
  2. ncbi The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes
    Lakshmanan Ganesh
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nature 426:853-7. 2003
    ..Through its effects on the proteasome, Murr1 acts as a genetic restriction factor that inhibits HIV-1 replication in lymphocytes, which could contribute to the regulation of asymptomatic HIV infection and the progression of AIDS...
  3. ncbi Regulation of the syncytin-1 promoter in human astrocytes by multiple sclerosis-related cytokines
    Giuseppe Mameli
    Section of Microbiology, Department of Biomedical Sciences, Center of Excellence for Biotechnology Development and Biodiversity Research, Sassari, Italy
    Virology 362:120-30. 2007
    ..Electrophoretic mobility shift and ChIP assays showed that TNFalpha enhances the binding of the p65 subunit of NF-kappaB, to its cognate site within the promoter...
  4. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
    ....
  5. doi Curcumin inhibits hepatitis C virus replication via suppressing the Akt-SREBP-1 pathway
    Kyeongjin Kim
    Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan, Republic of Korea
    FEBS Lett 584:707-12. 2010
    ..Collectively, our results indicate that curcumin can suppress HCV replication in vitro and may be potentially useful as novel anti-HCV reagents...
  6. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
    ..The functional consequences of NF-kappaB signaling may thus depend on number, period, and amplitude of oscillations...
  7. pmc Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression
    Satish L Deshmane
    Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania 19122, USA
    J Biol Chem 284:11364-73. 2009
    ....
  8. pmc Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes
    Xueqing Ba
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 285:11596-606. 2010
    ..ROS directly signaled the nuclear translocation of RelA (p65), NF-kappaB activation, and cytokine gene expression...
  9. pmc The cytoplasmic domain of the HIV-1 glycoprotein gp41 induces NF-κB activation through TGF-β-activated kinase 1
    Thomas S Postler
    New England Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical School, Southborough, MA 01772 9102, USA
    Cell Host Microbe 11:181-93. 2012
    ..These findings demonstrate an evolutionarily conserved role for gp41CD in activating NF-κB to promote infection...
  10. ncbi HIV-1 Vpr: a closer look at the multifunctional protein from the structural perspective
    RAMESH C PANDEY
    Department of Biotechnology, Indian Institute of Technology, Chennai, India
    Curr HIV Res 7:114-28. 2009
    ..The inter-relationship between different functions of Vpr is discussed in the context of structure. Based on bioinformatics analysis, we propose new targets for modulating Vpr functions, which need to be validated experimentally...
  11. ncbi Activation of the nuclear factor kappaB pathway by astrocyte elevated gene-1: implications for tumor progression and metastasis
    Luni Emdad
    Department of Pathology, Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA
    Cancer Res 66:1509-16. 2006
    ..AEG-1) in HeLa cells markedly increased binding of the transcriptional activator p50/p65 complex of NF-kappaB...
  12. ncbi Interleukin 1 activates STAT3/nuclear factor-kappaB cross-talk via a unique TRAF6- and p65-dependent mechanism
    Yasuhiro Yoshida
    New England Baptist Bone and Joint Institute, Beth Israel Deaconess Medical Center and the Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 279:1768-76. 2004
    ..We have observed that a specific type of kappa B DNA sequence motif supports both NF-kappaB p65 homodimer binding and cooperativity with non-tyrosine-phosphorylated STAT3...
  13. pmc Respiratory syncytial virus infection induces a reactive oxygen species-MSK1-phospho-Ser-276 RelA pathway required for cytokine expression
    Mohammad Jamaluddin
    Institute for Translational Sciences, University of Texas Medical Branch UTMB, Galveston, TX 77555 1060, USA
    J Virol 83:10605-15. 2009
    ....
  14. pmc Lysine methylation of the NF-κB subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κB signaling
    Dan Levy
    Department of Biology, Stanford University, Stanford, California, USA
    Nat Immunol 12:29-36. 2011
    ..Our findings establish a previously uncharacterized mechanism by which chromatin signaling regulates inflammation programs...
  15. pmc Human immunodeficiency virus type 1 viral protein R (Vpr) induces CCL5 expression in astrocytes via PI3K and MAPK signaling pathways
    Mohitkumar R Gangwani
    Division of Pharmacology and Toxicology, School of Pharmacy, University of Missouri, Kansas City, MO 64108, USA
    J Neuroinflammation 10:136. 2013
    ..The major objective of this study was to determine the effect of Vpr in induction of proinflammatory chemokine CCL5 in astrocytes and to define the underlying mechanism(s)...
  16. pmc IL-1beta-induced increase in intestinal epithelial tight junction permeability is mediated by MEKK-1 activation of canonical NF-kappaB pathway
    Rana Al-Sadi
    Department of Internal Medicine, MSC10 5550, University of New Mexico School of Medicine, Albuquerque Veterans Affairs Medical Center, Albuquerque, NM 87131 0001, USA
    Am J Pathol 177:2310-22. 2010
    ..In conclusion, our data show for the first time that MEKK-1 plays an integral role in IL-1β modulation of Caco-2 TJ barrier function by regulating the activation of the canonical NF-κB pathway and the MLCK gene...
  17. pmc The human immunodeficiency virus type 1 Tat protein up-regulates the promoter activity of the beta-chemokine monocyte chemoattractant protein 1 in the human astrocytoma cell line U-87 MG: role of SP-1, AP-1, and NF-kappaB consensus sites
    S P Lim
    Institute of Molecular and Cell Biology, Singapore 117609, Singapore
    J Virol 74:1632-40. 2000
    ..We postulate that a Tat-mediated increase in SP1 binding activities augments the binding of AP1 and NF-kappaB, leading to synergistic activation of the MCP-1 promoter...
  18. ncbi Tumor necrosis factor-alpha-induced IKK phosphorylation of NF-kappaB p65 on serine 536 is mediated through the TRAF2, TRAF5, and TAK1 signaling pathway
    Hiroaki Sakurai
    Department of Pathogenic Biochemistry, Institute of Natural Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama 930 0194, Japan
    J Biol Chem 278:36916-23. 2003
    ..The activation of NF-kappaB has been shown to be regulated by multiple phosphorylations of IkappaBs and the NF-kappaB p65 subunit...
  19. doi Cross talk between stimulated NF-kappaB and the tumor suppressor p53
    G Schneider
    Klinikum rechts der Isar, II, Medizinische Klinik, Technische Universitat Munchen, Munchen, Germany
    Oncogene 29:2795-806. 2010
    ..Here, we show that induction of replicational stress activates NF-kappaB p65 and triggers its interaction with p53 in the nucleus...
  20. pmc Human immunodeficiency virus-1 Tat activates NF-κB via physical interaction with IκB-α and p65
    Giuseppe Fiume
    Department of Experimental and Clinical Medicine, University of Catanzaro Magna Graecia, Viale Europa Germaneto, 88100 Catanzaro, Italy
    Nucleic Acids Res 40:3548-62. 2012
    ..Moreover, Tat associated with the p65 subunit of NF-κB and increased the p65 DNA-binding affinity and transcriptional activity...
  21. pmc IFNgamma restores breast cancer sensitivity to fulvestrant by regulating STAT1, IFN regulatory factor 1, NF-kappaB, BCL2 family members, and signaling to caspase-dependent apoptosis
    Yanxia Ning
    Lombardi Comprehensive Cancer Center, Georgetown University School of Medicine, Washington, DC 20057, USA
    Mol Cancer Ther 9:1274-85. 2010
    ..Increased IRF1 activation suppresses NF-kappaB p65 (RELA) activity, inhibits the expression of prosurvival (BCL2, BCL-W), and induces the expression of proapoptotic ..
  22. doi Suppressor of cytokine signaling 1 (SOCS1) limits NFkappaB signaling by decreasing p65 stability within the cell nucleus
    Julia Strebovsky
    Department of Infectious Diseases Medical Microbiology and Hygiene, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    FASEB J 25:863-74. 2011
    ..The NFκB component p65 is identified as an interaction partner for SOCS1 but not for other members of the SOCS family...
  23. ncbi Up-regulation of p300 binding and p50 acetylation in tumor necrosis factor-alpha-induced cyclooxygenase-2 promoter activation
    Wu Guo Deng
    Vascular Biology Research Center, Institute of Molecular Medicine and Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    J Biol Chem 278:4770-7. 2003
    ..We found basal binding of p300, p50/p65 NF-kappaB, cyclic AMP regulatory element-binding protein-2, CCAAT/enhancer-binding protein beta, and c-Jun...
  24. ncbi Bim and Noxa are candidates to mediate the deleterious effect of the NF-kappa B subunit RelA in cerebral ischemia
    Ioana Inta
    Department of Neurology, University of Heidelberg, 69120 Heidelberg, Germany
    J Neurosci 26:12896-903. 2006
    ..Thus, the deleterious function in cerebral ischemia is specific for the NF-kappaB subunit RelA and may be mediated through Bim and Noxa...
  25. pmc Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1
    B Majello
    Dipartimento di Genetica, Biologia Generale e Molecolare, Universita Federico II, Naples, Italy
    Nucleic Acids Res 22:4914-21. 1994
    ..We found that the cooperative interaction between NF-kappa B and Sp1 is highly specific, since neither Sp3 nor Sp4 is capable of cooperating with NF-kappa B...
  26. pmc Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing histone deacetylase 1-mediated deacetylation of RelA/p65 and promoting apoptosis
    Yuan Liu
    Department of Surgery, Box 800679, University of Virginia, Charlottesville, VA 22908 0679, USA
    Mol Cell Biol 26:8683-96. 2006
    ..In this report, we demonstrate that BRMS1 decreases the transactivation potential of RelA/p65 and ameliorates the expression of NF-kappaB-regulated antiapoptotic gene products...
  27. pmc Upregulation of Twist-1 by NF-kappaB blocks cytotoxicity induced by chemotherapeutic drugs
    Can G Pham
    The Ben May Institute for Cancer Research, The University of Chicago, 924 East 57th Street, Chicago, IL 60637, USA
    Mol Cell Biol 27:3920-35. 2007
    ..Indeed, our data indicate that this activity involves a control of inhibitory Bcl-2 phosphorylation. The data also suggest that Twist-1 and -2 play an important role in NF-kappaB-dependent chemoresistance...
  28. ncbi Hiv-1 Tat can substantially enhance the capacity of NIK to induce IkappaB degradation
    X Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Biochem Biophys Res Commun 286:587-94. 2001
    ..We propose a model to explain these collective experimental findings...
  29. pmc Novel transcription coactivator complex containing activating signal cointegrator 1
    Dong Ju Jung
    Department of Life Science, Pohang University of Science and Technology, Pohang 790 784, Korea
    Mol Cell Biol 22:5203-11. 2002
    ..activating protein 1 (AP-1), and nuclear factor kappaB (NF-kappaB) through direct binding to SRF, c-Jun, p50, and p65; and relieves the previously described transrepression between nuclear receptors and either AP-1 or NF-kappaB...
  30. ncbi Association of p65 and C/EBPbeta with HIV-1 LTR modulates transcription of the viral promoter
    Ruma Mukerjee
    Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania 19122, USA
    J Cell Biochem 100:1210-6. 2007
    ..site adjacent to the NF-kappaB site, the present study examined cooperative functional in vivo interaction of the p65 subunit of NF-kappaB and C/EBPbeta, and the impact of Tat in this event...
  31. pmc Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
    M R López-Huertas
    AIDS Immunopathology Unit, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain
    Nucleic Acids Res 38:3287-307. 2010
    ..Consequently, these modifications were greatly dependent on Tat second exon and they could be related to the anergy observed in HIV-1-infected T cells...
  32. doi BRAFV600E mutation, TIMP-1 upregulation, and NF-κB activation: closing the loop on the papillary thyroid cancer trilogy
    Alessandra Bommarito
    Sezione di Endocrinologia, Laboratorio di Endocrinologia Molecolare, Dipartimento di Biomedico di Medicina Interna e Specialistica DIBIMIS, University of Palermo, Italy
    Endocr Relat Cancer 18:669-85. 2011
    ..The recognition of this functional trilogy provides insight on how BRAF(V600E) determines cancer initiation, progression, and invasiveness in PTC, also identifying new therapeutic targets for the treatment of highly aggressive forms...
  33. ncbi Low dose ionising radiation leads to a NF-κB dependent decreased secretion of active IL-1β by activated macrophages with a discontinuous dose-dependency
    Barbara Lödermann
    Department of Radiation Oncology, University Hospital Erlangen, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Germany
    Int J Radiat Biol 88:727-34. 2012
    ..Little is known about whether and how low doses of X-ray treatment modulate the inflammatory phenotype of macrophages, especially the secretion of Interleukin-1beta (IL-1β)...
  34. doi HIV-1 Tat protein induces TNF-alpha and IL-10 production by human macrophages: differential implication of PKC-betaII and -delta isozymes and MAP kinases ERK1/2 and p38
    Kaoutar Leghmari
    Laboratoire d Immuno Virologie des Lentivirus des Primates, Université Paul Sabatier 118, Toulouse, France
    Cell Immunol 254:46-55. 2008
    ..However, p38 MAP kinase seems to be involved only in IL-10 and not TNF-alpha production...
  35. ncbi Analysis of the potential for HIV-1 Vpr as an anti-cancer agent
    Karrupiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Curr HIV Res 7:144-52. 2009
    ..In this report, we summarize the evidence supporting this hypothesis...
  36. doi HIV-1 Tat dysregulation of lipopolysaccharide-induced cytokine responses: microbial interactions in HIV infection
    Howard C H Yim
    Cytokine Biology Group, Department of Paediatrics and Adolescent Medicine, China bBio Screening Unit, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
    AIDS 23:1473-84. 2009
    ..To examine whether the HIV-1 Tat protein impairs the lipopolysaccharide (LPS)-induced cytokine responses...
  37. pmc Inhibiting TRAF2-mediated activation of NF-kappaB facilitates induction of AP-1
    Sunil K Manna
    Laboratory of Immunology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500 001, India
    J Biol Chem 285:11617-27. 2010
    ..These results suggest novel approaches to design of P(3)-25 as an anti-cancer/inflammatory drug for therapy through regulation of the TRAF2 pathway...
  38. ncbi Thrombin stimulation of vascular adhesion molecule-1 in endothelial cells is mediated by protein kinase C (PKC)-delta-NF-kappa B and PKC-zeta-GATA signaling pathways
    Takashi Minami
    Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 278:6976-84. 2003
    ..In electrophoretic mobility shift assays, thrombin-mediated induction of NF-kappaB p65 binding to two NF-kappaB motifs in the upstream promoter region of VCAM-1 was blocked by LY294002 and rottlerin, ..
  39. ncbi Homocysteine induces VCAM-1 gene expression through NF-kappaB and NAD(P)H oxidase activation: protective role of Mediterranean diet polyphenolic antioxidants
    Maria Annunziata Carluccio
    Consiglio Nazionale delle Ricerca Institute of Clinical Physiology, University of Lecce, Lecce, Italy
    Am J Physiol Heart Circ Physiol 293:H2344-54. 2007
    ..Hcy-induced NF-kappaB activation was confirmed by EMSA, as shown by the nuclear translocation of its p65 (RelA) subunit and the degradation of the inhibitors IkappaB-alpha and IkappaB-beta by Western analysis...
  40. pmc NF-κB hyper-activation by HTLV-1 tax induces cellular senescence, but can be alleviated by the viral anti-sense protein HBZ
    Huijun Zhi
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    PLoS Pathog 7:e1002025. 2011
    ..Finally, inactivation of the senescence checkpoint can facilitate persistent NF-κB activation and leukemogenesis...
  41. pmc Activation of Syk by protein kinase C-delta regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via tyrosine phosphorylation of RelA/p65
    Kaiser M Bijli
    Department of Pediatrics Neonatology, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Biol Chem 283:14674-84. 2008
    ..its transactivation potential and that this response is associated with tyrosine phosphorylation of RelA/p65. Thus, these data unveil a novel pathway in which Syk signals downstream of PKC-delta to mediate thrombin induced ..
  42. ncbi I kappa B: a specific inhibitor of the NF-kappa B transcription factor
    P A Baeuerle
    Whitehead Institute for Biomedical Research, MA 02142
    Science 242:540-6. 1988
    ..The data show the existence of a phorbol ester-responsive regulatory protein that acts by controlling the DNA binding activity and subcellular localization of a transcription factor...
  43. ncbi Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300
    H Zhong
    Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Cell 1:661-71. 1998
    The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA)...
  44. ncbi MDM2 induces NF-kappaB/p65 expression transcriptionally through Sp1-binding sites: a novel, p53-independent role of MDM2 in doxorubicin resistance in acute lymphoblastic leukemia
    Lubing Gu
    Division of Pediatric Hematology Oncology BMT, Emory University School of Medicine, Atlanta, GA 30322, USA
    Blood 99:3367-75. 2002
    ..Here we report that MDM2 can induce expression of the p65 subunit of NF-kappaB, which is an anti-apoptotic factor expressed in certain neoplastic cells in response to ..
  45. pmc SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span
    Tiara L A Kawahara
    Program in Epithelial Biology, Division of Endocrinology, Gerontology, and Metabolism, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 136:62-74. 2009
    ..We propose that SIRT6 attenuates NF-kappaB signaling via H3K9 deacetylation at chromatin, and hyperactive NF-kappaB signaling may contribute to premature and normal aging...
  46. ncbi Association of interferon regulatory factor-1, nucleophosmin, nuclear factor-kappaB, and cyclic AMP response element binding with acquired resistance to Faslodex (ICI 182,780)
    Zhiping Gu
    Vincent T Lombardi Cancer Center and Department of Oncology, Georgetown University School of Medicine, Washington, DC 20007, USA
    Cancer Res 62:3428-37. 2002
    ..Thus, some breast cancer cells may survive antiestrogen treatment by bypassing specific growth inhibitory signals induced by antagonist-occupied estrogen receptors...
  47. ncbi Recruitment of NF-kappaB into mitochondria is involved in adenine nucleotide translocase 1 (ANT1)-induced apoptosis
    Monica Zamora
    Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Diagonal 645, 08028 Barcelona, Spain
    J Biol Chem 279:38415-23. 2004
    ..Accordingly, co-expression of p65 partially interferes with the proapoptotic effect of ANT1 overexpression...
  48. ncbi Mitogenic and antiapoptotic role of constitutive NF-kappaB/Rel activity in pancreatic cancer
    Susanne Liptay
    Department of Pediatrics, University of Ulm, Ulm, Germany
    Int J Cancer 105:735-46. 2003
    ..NF-kappaB/Rel-binding activity consists of NF-kappaB1(p50) and RelA(p65)...
  49. doi Nucleolar targeting of RelA(p65) is regulated by COMMD1-dependent ubiquitination
    Hazel C Thoms
    Colon Cancer Genetics Group, University of Edinburgh Cancer Research Centre and MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom
    Cancer Res 70:139-49. 2010
    ..These findings have relevance to the design of chemopreventative/anticancer agents that act by targeting RelA to the nucleolar compartment...
  50. pmc Nucleolar NF-κB/RelA mediates apoptosis by causing cytoplasmic relocalization of nucleophosmin
    N Khandelwal
    University of Edinburgh Cancer Research Centre and MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, Scotland, UK
    Cell Death Differ 18:1889-903. 2011
    ..These findings are relevant to the design of novel anticancer agents that target RelA to this compartment...
  51. pmc Negative regulation of NF-kappaB signaling by PIAS1
    Bin Liu
    Division of Hematology Oncology, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095 1678, USA
    Mol Cell Biol 25:1113-23. 2005
    ..Upon cytokine stimulation, the p65 subunit of NF-kappaB translocates into the nucleus, where it interacts with PIAS1...
  52. pmc Vascular cell adhesion molecule-1 expression in human intestinal microvascular endothelial cells is regulated by PI 3-kinase/Akt/MAPK/NF-kappaB: inhibitory role of curcumin
    David G Binion
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Am J Physiol Gastrointest Liver Physiol 297:G259-68. 2009
    ..Nuclear factor-kappaB (NF-kappaB) activation and nuclear translocation of its p65 subunit were determined...
  53. pmc Structural basis of HIV-1 activation by NF-kappaB--a higher-order complex of p50:RelA bound to the HIV-1 LTR
    James C Stroud
    Molecular and Computational Biology, Department of Biological Sciences, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089 2910, USA
    J Mol Biol 393:98-112. 2009
    ..This phenomenon is unlikely restricted to the HIV-1 LTR but probably represents a general mechanism for the function of composite DNA elements in transcription...
  54. pmc A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling
    Nan Gao
    Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya 467 8601, Japan
    J Biol Chem 285:28097-104. 2010
    ..agents inhibited the NF-kappaB-dependent reporter gene expression, blocked the interaction of PKAc and p65 subunit of NF-kappaB, and attenuated PKA-dependent phosphorylation of p65 on Ser-276...
  55. pmc Ionizing radiation-induced NF-kappaB activation requires PARP-1 function to confer radioresistance
    S J Veuger
    Northern Institute for Cancer Research, Medical School, University of Newcastle, Newcastle upon Tyne, Tyne and Wear, UK
    Oncogene 28:832-42. 2009
    ..activated NF-kappaB, as well as mouse embryonic fibroblasts (MEFs) proficient or deficient for PARP-1 or NF-kappaB p65. In the breast cancer cell lines, AG14361 had no effect on IR-induced degradation of IkappaBalpha or nuclear ..
  56. pmc In vitro and in vivo binding of human immunodeficiency virus type 1 Tat protein and Sp1 transcription factor
    K T Jeang
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
    J Virol 67:6224-33. 1993
    ..These experiments extend previous genetic experiments and suggest a direct interaction between Tat and Sp1 during transactivation...
  57. ncbi Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II
    D Wang
    Department of Biology, Curriculum in Genetics and Molecular Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 275:32592-7. 2000
    ..Previously, we have shown that TNFalpha induces RelA/p65 phosphorylation at serine 529 and that this inducible phosphorylation increases NF-kappaB transcriptional activity ..
  58. pmc The p65 (RelA) subunit of NF-kappaB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression
    B P Ashburner
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 21:7065-77. 2001
    ..Here we show that the transactivation function of NF-kappaB is also regulated through interaction of the p65 (RelA) subunit with histone deacetylase (HDAC) corepressor proteins...
  59. ncbi BRCA1 augments transcription by the NF-kappaB transcription factor by binding to the Rel domain of the p65/RelA subunit
    Miriam Benezra
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 278:26333-41. 2003
    ..BRCA1 protein is involved in apoptosis and growth/tumor suppression. In this study, we present evidence that p65/RelA, one of the two subunits of the transcription factor NF-kappaB, binds to the BRCA1 protein...
  60. ncbi Proteolytic cleavage of the p65-RelA subunit of NF-kappaB during poliovirus infection
    Nickolay Neznanov
    Department of Molecular Genetics, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 280:24153-8. 2005
    ..However, at later stages of poliovirus replication the p65-RelA component of the NF-kappaB complex undergoes a specific cleavage that coincides with the onset of intensive ..
  61. ncbi Overexpression of p65/RelA potentiates curcumin-induced apoptosis in HCT116 human colon cancer cells
    Gavin P Collett
    Department of Surgery, Centre for Cancer Research and Cell Biology, Queen s University Belfast, Belfast BT12 6BJ, UK
    Carcinogenesis 27:1285-91. 2006
    ..The p65/RelA subunit of NFkappaB may influence cell death, in part by activation of NFkappaB anti-apoptotic target genes ..
  62. pmc Phosphorylation of p65(RelA) on Ser(547) by ATM represses NF-κB-dependent transcription of specific genes after genotoxic stress
    Hélène Sabatel
    Laboratory of Virology and Immunology, GIGA R, Signal Transduction Unit, University of Liege, Liege, Belgium
    PLoS ONE 7:e38246. 2012
    ..In this manuscript, a direct interaction between p65(RelA) and the N-terminal extremity of ATM is reported...
  63. ncbi The nuclear receptor chicken ovalbumin upstream promoter transcription factor interacts with HIV-1 Tat and stimulates viral replication in human microglial cells
    O Rohr
    Unité 338 INSERM, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France
    J Biol Chem 275:2654-60. 2000
    ..These results suggest that COUP-TF is an essential transcription factor involved in HIV-1 expression in microglia and reveal a novel interplay of Tat and COUP-TF during regulation of viral expression...
  64. ncbi T cell activation-induced and HIV tat-enhanced CD95(APO-1/Fas) ligand transcription involves NF-kappaB
    M Li-Weber
    Tumor Immunology Program, German Cancer Research Center DKFZ, Heidelberg, Germany
    Eur J Immunol 30:661-70. 2000
    ..Our studies provide evidence that Tat-enhanced CD95L expression is regulated at least in part by the NF-kappaB sites of the promoter...
  65. ncbi Silencing mediator of retinoic acid and thyroid hormone receptors, as a novel transcriptional corepressor molecule of activating protein-1, nuclear factor-kappaB, and serum response factor
    S K Lee
    Center for Ligand and Transcription, Chonnam National University, Kwangju 500 757, Korea
    J Biol Chem 275:12470-4. 2000
    ..of SMRT directly interacted with SRF, the AP-1 components c-Jun and c-Fos, and the NFkappaB components p50 and p65, as demonstrated by the yeast and mammalian two hybrid tests as well as the glutathione S-transferase pull down ..
  66. ncbi c-Src interacts with and phosphorylates RelA/p65 to promote thrombin-induced ICAM-1 expression in endothelial cells
    Kaiser M Bijli
    Department of Pediatrics, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Am J Physiol Lung Cell Mol Physiol 292:L396-404. 2007
    ..activation of c-Src is crucial in signaling thrombin-induced ICAM-1 expression via tyrosine phosphorylation of RelA/p65. Stimulation of human umbilical vein endothelial cells with thrombin resulted in a time-dependent activation of c-..
  67. ncbi Human immunodeficiency virus (HIV) type 1 Vpr induces differential regulation of T cell costimulatory molecules: direct effect of Vpr on T cell activation and immune function
    Narasimhan J Venkatachari
    Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, 130 DeSoto Street, Pittsburgh, PA 15261, USA
    Virology 358:347-56. 2007
    ..Together these results support that HIV-1 Vpr selectively dysregulates the immune functions at multiple levels and exerts its inhibitory effects in the presence of other viral proteins...
  68. pmc LMP1-augmented kappa intron enhancer activity contributes to upregulation expression of Ig kappa light chain via NF-kappaB and AP-1 pathways in nasopharyngeal carcinoma cells
    Haidan Liu
    Cancer Research Institute, Xiangya School of Medicine, Central South University, Xiangya Road 110, Changsha, Hunan 410078, PR China
    Mol Cancer 8:92. 2009
    ....
  69. doi Effect of RNAi-induced down regulation of nuclear factor kappa-B p65 on acute monocytic leukemia THP-1 cells in vitro and vivo
    Chunmei Wang
    Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, and Department of Epidemiology and Biostatistics, School of Public Health, Zhengzhou University, No 1 Jianshe East Road, Zhengzhou, 450052, Henan, China
    Mol Cell Biochem 359:125-33. 2012
    NF-κB p65 is found constitutively active in acute monocytic leukemia, and has been considered an important factor for poor prognosis. Therefore, develop specifically target p65 inhibitors will be substantial interest...
  70. ncbi Inhibition of the RelA(p65) NF-kappaB subunit by Egr-1
    N R Chapman
    Department of Biochemistry, Division of Gene Expression and Regulation, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH Scotland, United Kingdom
    J Biol Chem 275:4719-25. 2000
    Induction of transcription from the human immunodeficiency virus 1 long terminal repeat by the RelA (p65) NF-kappaB subunit has been shown to be dependent upon an interaction with the zinc finger DNA-binding domain of Sp1...
  71. ncbi The human immunodeficiency virus-1 Tat protein activates human umbilical vein endothelial cell E-selectin expression via an NF-kappa B-dependent mechanism
    Adela Cota-Gomez
    Webb Waring Institute for Cancer, Aging and Antioxidant Research, The Department of Microbiology, School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 277:14390-9. 2002
    ..Either Tat or TNF activated p65 translocation and binding to an oligonucleotide containing the E-selectin kappaB site 3 sequence...
  72. pmc Molecular mechanism of hTid-1, the human homolog of Drosophila tumor suppressor l(2)Tid, in the regulation of NF-kappaB activity and suppression of tumor growth
    Hua Cheng
    Department of Cancer Biology, University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Mol Cell Biol 25:44-59. 2005
    ..Collectively, the data suggest that hTid-1 represses the activity of NF-kappaB through physical and functional interactions with the IKK complex and IkappaB and, in doing so, it modulates cell growth and death...
  73. doi Akt/Nox2/NF-κB signaling pathway is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation
    Hong Sheng Zhang
    Beijing University of Technology, District of Chaoyang, China
    Arch Biochem Biophys 505:266-72. 2011
    ..inhibition of NADPH oxidase by both pharmacologic NADPH oxidase inhibitors and by siRNA Nox2 attenuated Tat-induced p65 phosphorylation and IKK phosphorylation...
  74. doi The tumor marker Fascin is strongly induced by the Tax oncoprotein of HTLV-1 through NF-kappaB signals
    Andrea K Kress
    Institute of Clinical and Molecular Virology, Friedrich Alexander Universitat Erlangen Nurnberg, Schlossgarten 4, Erlangen, Germany
    Blood 117:3609-12. 2011
    ..Nuclear factor-κB signals were important for Tax-mediated transcriptional regulation of Fascin in T cells. This suggests that Fascin up-regulation by Tax contributes to the development of HTLV-1-associated pathogenesis...
  75. pmc HIV-1 Tat potentiates TNF-induced NF-kappa B activation and cytotoxicity by altering the cellular redox state
    M O Westendorp
    Division of Immunogenetics, German Cancer Research Center, Heidelberg
    EMBO J 14:546-54. 1995
    ..abstract truncated at 250 words)..
  76. ncbi Phosphorylation of serine 468 by GSK-3beta negatively regulates basal p65 NF-kappaB activity
    Holger Buss
    Institute of Pharmacology, Medical School Hannover, Carl Neuberg Strasse 1, D 30625 Hannover, Germany
    J Biol Chem 279:49571-4. 2004
    ..activity of NF-kappaB is controlled at several levels including the phosphorylation of the strongly transactivating p65 (RelA) subunit...
  77. ncbi Phosphorylation of RelA/p65 on serine 536 defines an I{kappa}B{alpha}-independent NF-{kappa}B pathway
    Carl Y Sasaki
    Laboratory of Immunology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 280:34538-47. 2005
    The association of the NF-kappaB p65/p50 dimer with IkappaBalpha plays a pivotal role in regulating its nuclear translocation and gene transcription...
  78. ncbi Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65/RelA
    Akihide Ryo
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, HIM 1047, Boston, MA 02215, USA
    Mol Cell 12:1413-26. 2003
    ..NF-kappaB function is regulated by Pin1-mediated prolyl isomerization and ubiquitin-mediated proteolysis of its p65/RelA subunit...
  79. ncbi Insufficient p65 phosphorylation at S536 specifically contributes to the lack of NF-kappaB activation and transformation in resistant JB6 cells
    Jing Hu
    Gene Regulation Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute at Frederick, MD 21702, USA
    Carcinogenesis 25:1991-2003. 2004
    ..Deficient activation of p65 contributes to the lack of NF-kappaB activation in transformation-resistant (P-) cells...
  80. ncbi Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation
    Ching Aeng Lim
    Laboratory of Immunology and Virology, Genome Institute of Singapore, 138672 Singapore
    Mol Cell 27:622-35. 2007
    ..Global gene expression profiling subsequently revealed a spectrum of NF-kappaB target genes that are positively regulated by E2F1, further demonstrating the critical role of E2F1 in the Toll-like receptor 4 pathway...
  81. pmc Control of stochastic gene expression by host factors at the HIV promoter
    John C Burnett
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California, United States of America
    PLoS Pathog 5:e1000260. 2009
    ..These results may have biomedical implications for the treatment of HIV latency...
  82. pmc Essential role for epidermal growth factor receptor in glutamate receptor signaling to NF-kappaB
    Raquel Sitcheran
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 28:5061-70. 2008
    ..induces phosphorylation, nuclear accumulation, DNA binding, and transcriptional activation function of glial p65. The glutamate-induced activation of NF-kappaB requires calcium-dependent IkappaB kinase alpha (IKKalpha) and ..
  83. pmc IkappaB kinase alpha-mediated derepression of SMRT potentiates acetylation of RelA/p65 by p300
    Jamie E Hoberg
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 26:457-71. 2006
    ..However, in the latter phase, chromatin-bound IKKalpha coordinates the simultaneous phosphorylation of RelA/p65(S536) and SMRT(S2410) as detected by chromatin immunoprecipitation (ChIP) assays...
  84. pmc High expression of RelA/p65 is associated with activation of nuclear factor-kappaB-dependent signaling in pancreatic cancer and marks a patient population with poor prognosis
    W Weichert
    Institute of Pathology, Charite University Hospital, Berlin, Germany
    Br J Cancer 97:523-30. 2007
    ..However, information on the expression of RelA/p65, the major transcription activating NF-kappaB subunit, in these carcinomas and possible correlations thereof with ..
  85. pmc The expression of the interleukin 6 gene is induced by the human immunodeficiency virus 1 TAT protein
    G Scala
    Department of Biochemistry and Biomedical Technology, Medical School, University Federico II, Naples, Italy
    J Exp Med 179:961-71. 1994
    ..These results suggest that TAT protein may play a role in the pathogenesis of some HIV1-associated diseases by modulating the expression of host cellular genes...
  86. pmc Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling
    Fumitaka Fujita
    Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, Mizuho ku, Nagoya 467 8601, Japan
    Mol Cell Biol 23:7780-93. 2003
    ..Interestingly, the NAK-NAP1 complex itself effectively phosphorylated serine 536 of the p65/RelA subunit of NF-kappaB, and this activity was stimulated by tumor necrosis factor alpha (TNF-alpha)...
  87. pmc COMMD1 promotes the ubiquitination of NF-kappaB subunits through a cullin-containing ubiquitin ligase
    Gabriel N Maine
    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    EMBO J 26:436-47. 2007
    ..Our data uncover that ubiquitination and degradation of NF-kappaB subunits by this COMMD1-containing ubiquitin ligase is a novel and critical mechanism of regulation of NF-kappaB-mediated transcription...
  88. pmc The ING4 tumor suppressor attenuates NF-kappaB activity at the promoters of target genes
    Susan Nozell
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
    Mol Cell Biol 28:6632-45. 2008
    ..and NF-kappaB bind simultaneously at NF-kappaB-regulated promoters, and this binding correlates with reductions in p65 phosphorylation, p300, and the levels of acetylated histones and H3-Me3K4, while enhancing the levels of HDAC-1 at ..
  89. ncbi Promoter-dependent effect of IKKalpha on NF-kappaB/p65 DNA binding
    Geoffrey Gloire
    GIGA Research, Virology Immunology, and Medical Chemistry Units, University of Liege, B 4000 Liege, Belgium
    J Biol Chem 282:21308-18. 2007
    ..and reexpressing IKKalpha after retroviral transduction, we demonstrate that IKKalpha contributes to NF-kappaB/p65 DNA binding activity on an exogenous kappaB element and on some, but not all, endogenous NF-kappaB-target promoters...
  90. pmc Nucleolar sequestration of RelA (p65) regulates NF-kappaB-driven transcription and apoptosis
    Lesley A Stark
    Colon Cancer Genetics Group, University of Edinburgh Division of Oncology, MRC Human Genetics Unit, Western General Hospital, Crewe Rd, Edinburgh, Scotland
    Mol Cell Biol 25:5985-6004. 2005
    ..These data contribute to the understanding of the complexities of NF-kappaB function and have considerable relevance to cancer prevention and therapy...
  91. doi RelA/NF-kappaB transcription factor associates with alpha-actinin-4
    Vladimir N Babakov
    Department of Cell Cultures, Institute of Cytology, St Petersburg, Russian Federation
    Exp Cell Res 314:1030-8. 2008
    ..After a 30-min stimulation with EGF or TNF-alpha, alpha-actinin-4 and p65 were found in the nucleus...
  92. ncbi The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function
    P O Hassa
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Biol Chem 276:45588-97. 2001
    ..PARP-1 was also required for p65-mediated transcriptional activation...
  93. pmc Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB
    Lin Feng Chen
    Gladstone Institute of Virology and Immunology, University of California at San Francisco, San Francisco, CA 94141, USA
    EMBO J 21:6539-48. 2002
    ..Together, these findings highlight how site-specific acetylation of RelA differentially regulates distinct biological activities of the NF-kappaB transcription factor complex...
  94. pmc Phosphorylation of NF-kappaB p65 at Ser468 controls its COMMD1-dependent ubiquitination and target gene-specific proteasomal elimination
    Hui Geng
    Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Justus Liebig University, D 35392 Giessen, Germany
    EMBO Rep 10:381-6. 2009
    ..phosphorylation/ubiquitination switch in the NF-kappaB network that controls the stability of the transactivating p65 subunit...
  95. pmc Protein phosphatase 2A interacts with and directly dephosphorylates RelA
    J Yang
    Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175, USA
    J Biol Chem 276:47828-33. 2001
    ..and Richmond, A. (2001) Cancer Res. 61, 4901-4909) could be due, at least in part, to the deficiency of PP2A, which exhibits decreased dephosphorylation of NF-kappa B/RelA...
  96. doi CD28 costimulation regulates FOXP3 in a RelA/NF-κB-dependent mechanism
    Marzia Soligo
    Department of Biology and Biotechnology C Darwin, University Sapienza of Rome, Italy
    Eur J Immunol 41:503-13. 2011
    ..In conclusion, these data suggest a new mechanism by which FOXP3 is activated and supports the critical role of CD28 in the regulation of peripheral tolerance...
  97. pmc Human immunodeficiency virus type 1 tat protein activates transcription factor NF-kappaB through the cellular interferon-inducible, double-stranded RNA-dependent protein kinase, PKR
    F Demarchi
    Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy
    J Virol 73:7080-6. 1999
    ..Activation of NF-kappaB by Tat required integrity of the basic domain of Tat; previous studies have indicated that this domain is necessary for specific Tat-PKR interaction...
  98. pmc A novel form of the RelA nuclear factor kappaB subunit is induced by and forms a complex with the proto-oncogene c-Myc
    Neil R Chapman
    Division of Gene Expression and Regulation, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 366:459-69. 2002
    ..We have discovered that both c-Myc and v-Myc can induce a previously undescribed, truncated form of the RelA(p65) NF-kappaB subunit, RelA(p37)...
  99. ncbi HIV-Tat protein induces oxidative and inflammatory pathways in brain endothelium
    Michal Toborek
    Department of Surgery, Animal Sciences and Neurology, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
    J Neurochem 84:169-79. 2003
    ..These changes can directly lead to disruption of the BBB. Thus, Tat can play an important role in the development of detrimental vascular changes in the brains of HIV-infected patients...
  100. pmc Essential role of RelA Ser311 phosphorylation by zetaPKC in NF-kappaB transcriptional activation
    Angeles Duran
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Canto Blanco, 28049 Madrid, Spain
    EMBO J 22:3910-8. 2003
    ..These results demonstrate the mechanism whereby zetaPKC regulates NF-kappaB activation in vivo...
  101. pmc Activation of p300 histone acetyltransferase activity is an early endothelial response to laminar shear stress and is essential for stimulation of endothelial nitric-oxide synthase mRNA transcription
    Wei Chen
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30022, USA
    J Biol Chem 283:16293-8. 2008
    ..mRNA transcription by laminar shear stress is dependent on nuclear factor kappa B (NFkappaB) subunits p50 and p65 binding to a shear stress response element (SSRE) in the human eNOS promoter and that mutation of the SSRE ..

Research Grants75

  1. NF-kappaB Regulation by Androgen Receptor
    ALBERT BALDWIN JR; Fiscal Year: 2004
    ..with an inhibitory protein known as IkappaB and another mechanism involves direct phosphorylation of the p65 subunit of NF-kappaB which controls transactivation potential...
  2. Use p105(sr) to study p50 homodimer in skin tumor/cancer
    Li Lin; Fiscal Year: 2009
    ..We will also test whether introducing p105(sr) into skin tumor cells facilitates apoptosis, and whether this approach significantly affects physiologic functions of normal keratinocytes. ..
  3. Hypothermia enhances inflammatory cytokine expression via NF-kappa B
    Karen D Fairchild; Fiscal Year: 2010
    ..Our preliminary data show that HT alters key phosphorylation and acetylation events which favor association of the p65 transactivation component of NF-/kB with cytokine gene promoters on chromatin...
  4. Control of NF-kappaB Activity
    ALBERT SIDNEY BALDWIN; Fiscal Year: 2013
    ..growth factor (EGF)-induced histone H3 serl0 phosphorylation and that GSK-313 controls the phosphorylation of the p65 subunit on ser536...
  5. Ezra Burstein; Fiscal Year: 2015
    ..Altogether, this proposal will tackle a critically significant problem that will propel the NF-?B field forward by providing important insights into homeostatic mechanisms that prevent persistent inflammation. ..
  6. Gourisankar Ghosh; Fiscal Year: 2016
    ..The combinatorial NF-?B dimers are formed from five family members;p50, p52, RelA (p65), cRel and RelB...
  7. Inflammatory Cytokines Link NF-kappaB with Bim Degradation and Metastasis
    Marty W Mayo; Fiscal Year: 2012
    ..Preliminary data presented in this proposal indicate that the p65 component of NF-?B provides a novel avenue of cell survival by blocking detachment from the extracellular matrix...
  8. Lin Feng Chen; Fiscal Year: 2014
    ..pylori-initiated inflammatory diseases and cancer. ..
  9. CHARLES EMORY MCCALL; Fiscal Year: 2014
    ..silencing signature requires Toll-like receptor 4 (TLR4) induction of NF-kappa B factor RelB that disrupts p65 promoter binding, directs histone H3K9 di-methylation by G9a to provide a binding site of heterochromatin protein 1 ..
  10. Lung-Liver Axis During Pneumonia
    Lee Quinton; Fiscal Year: 2012
    ..Completion of these studies will demonstrate whether mechanisms regulating APP expression in the liver may be novel therapeutic targets for patients with pneumonia or other severe infections. ..
  11. NF kappa B Activity in Skin Inflammation and Carcinogenesis
    Brian Poligone; Fiscal Year: 2013
    ..We have constructed a novel mouse model to examine the NF-KB pathway in skin. The homozygous p65 S276D (PD) knock-in mouse contains a mutant form of NF-KB that increases the basal transcriptional activation of NF-..
  12. NF-kappaB Inhibitors and Differentiation-Inducers in Leukemia
    Steven Grant; Fiscal Year: 2011
    ..Insights generated during the preceding funding period have led us to the hypothesis that NF-kappaB/p65 acetylation/nuclear translocation/activation plays a pivotal role in protecting leukemic cells from HDACI-mediated ..
  13. Role of IKK in NSCLC: Modulation of SMRT and NF-kappaB
    Marty W Mayo; Fiscal Year: 2012
    ..Aim 2 will determine the role of p65 as an activator or repressor of polycomb-mediated gene expression...
  14. ALBERT SIDNEY BALDWIN; Fiscal Year: 2014
    ..test for a role of NF-?B in oncogene-induced tumorigenesis, we show here that loss of the gene encoding the RelA/p65 NF-?B subunit suppresses Ras-induced tumorigenesis in a lung tumor model...
  15. Wiring the UV Signaling Circuitry
    Anning Lin; Fiscal Year: 2012
    ..Specifically, RelA/p65, which is a major transactivating subunit of the NF-kappaB family, in its pre-existing nuclear form controls ..
  16. Regulation of NF-kappaB transactivation function
    BRIAN ASHBURNER; Fiscal Year: 2009
    ..These signals are thought to target the C-terminal transcriptional activation domain (TAD) of the p65 subunit of NF-kB and likely function to regulate the association of transcriptional coactivator and corepressor ..
  17. Anna K Mapp; Fiscal Year: 2016
    ..small (drug-like) molecules that inhibit the function of two transcriptional activators, ESX and NF- kappaB (p50/p65 heterodimer) implicated in approximately 30% of breast cancers...
  18. Chamomile as Medicine for Prostate cancer
    Sanjay Gupta; Fiscal Year: 2009
    ..Our recent studies (Neoplasia Vol.6 No.4, 2004) have shown that NF-kappaB/p65 is constitutively activated in human prostate adenocarcinoma and correlates with disease progression...
  19. Regulation of NF-kB transcriptional activity
    Sankar Ghosh; Fiscal Year: 2009
    ..However, studies carried out in our laboratory have demonstrated that the p65 subunit of NF-kappaB has to be phosphorylated to become competent for transcription...
  20. Interleukin-8 Regulation by Proteasome and Nuclear IkB in Cancer and Inflammation
    Ivana Vancurova; Fiscal Year: 2013
    ..The central hypothesis is that S536 p65 phosphorylation, specificity of the DNA sequence of the NF?B binding site, and/or the transcription factor EGR-1 ..
  21. Suzanne Gaudet; Fiscal Year: 2016
    ..Finally, we will use protein-binding microarrays to ask how competition between p65-p50 heterodimers and p50-p50 homodimers contributes to decoding NF-kB activation and to cell line-to-cell line ..
  22. Regulation of Innate Immunity by Pyrin Proteins
    Jonathan A Harton; Fiscal Year: 2012
    ..Specifically, we have identified a second human POP (POP2) which inhibits NF-kB p65 activity, interacts with ASC, and prevents the activation of a number of NLR activated inflammasomes...
  23. Richard J Lamont; Fiscal Year: 2016
    ..JAK2, JAK3, and TYK2 recruitment to TLR2;(5) inhibition of JAK2 resulted in the abrogated activation of the NF-:B p65 signaling pathway;(6) JAK2 was activated in mice challenged with P...
  24. FADIE THOMAS COLEMAN; Fiscal Year: 2014
    ....
  25. Hepatic Responses to Pulmonary Infections
    REBECCA SHARON CHINTHRAJAH; Fiscal Year: 2011
    ..Understanding the activation pathways and the biological significance of this acute phase response can give us insights into potential mechanisms for prevention and treatment of this devastating illness. ..
  26. Brendan F Boyce; Fiscal Year: 2016
    ..Expression of these is essential for osteoclast formation. RANKL/RANK signals through a canonical NF-?B1/p65 and an alternative NF-?B2/RelB pathway...
  27. Cytoprotective cytokine signaling and reperfusion injury
    DOUGLAS LOWELL MANN; Fiscal Year: 2010
    ..First, are the cytoprotective effects of TRAF2 mediated by activation of the type 1 NF-?B pathway, and if so are p65 and p50 homo or heterodimers involved (Specific Aim 1)? Second, is dysferlin necessary for the cytoprotective ..
  28. Mechanisms of Immune Modulation by Abscisic Acid
    Josep Bassaganya Riera; Fiscal Year: 2010
    ..The mechanistic understanding of the immune modulatory properties of botanicals such as ABA will aid in the development of more efficacious CAM approaches for modulating immune function and ameliorating infectious diseases. ..
  29. ISTVAN STEVEN BOLDOGH; Fiscal Year: 2014
    ....
  30. Li Gan; Fiscal Year: 2016
    ..These studies will also lay the foundation for our long-term goal of developing SIRT1-enhancing strategies as a new therapeutic approach for AD. ..
  31. NAOMI MIRIAM HAMBURG; Fiscal Year: 2014
    ..eNOS expression, insulin-mediated eNOS phosphorylation) and evidence of endothelial inflammatory activation (I:2, p65, ICAM expression) in endothelial cells freshly isolated from human subjects to the presence of diabetes and to the ..
  32. Wendell G Yarbrough; Fiscal Year: 2016
    ..We propose to test these hypotheses in this grant. ..
  33. SIMPL-a TNFalpha-specific transcriptional co-activator
    Maureen A Harrington; Fiscal Year: 2010
    ..This proposal focuses on a factor we identified termed SIMPL. SIMPL is a TNF1 regulated p65 specific co- activator...
  34. Joseph P Mizgerd; Fiscal Year: 2015
    ..This new knowledge will guide further studies aiming to identify people who are especially susceptible and bacteria that are especially virulent, in order to better prevent and treat pneumococcal infections. ..
  35. Hypercapnia and pulmonary host defense
    Khalilah L Gates; Fiscal Year: 2010
    ..preliminary studies show that hypercapnia suppresses macrophage synthesis of multiple cytokines regulated by NF-:B p65/Rel A that are critical for host defense, and also inhibits phagocytosis...
  36. Fabeha Fazal; Fiscal Year: 2014
    ..The basis of ICAM-1 expression involves activation of RelA/p65 subunit of the transcription factor NF-(B...
  37. ROLE OF CSF-1 IN OSTEOCLAST FUNCTION
    KARL LEONARD INSOGNA; Fiscal Year: 2013
    ..We have discovered that BCA3 regulates CSF1-induced cytoskeletal remodeling and also augments NF:?B p65 signaling. In the second Specific Aim, the role of BCA3 in mediating both CSF1 and RANKL signaling will be explored...
  38. Ruxana T Sadikot; Fiscal Year: 2016
    ..three interrelated specific aims:1) Define the molecular mechanisms by which TREM-1 expression is regulated by p65 and PU.1 in vitro in macrophages in response to P.aeruginosa...
  39. J Marc Simard; Fiscal Year: 2016
    ..activation of the mechano-sensitive transcription factors, specificity protein 1 (Sp1) and nuclear factor kappaB (p65), and the time-course and cellular localization for the cell death molecules, SUR1, transient receptor potential ..
  40. Intestinal Microbiota, Diet and Risk of Colorectal Adenomas
    Robert S Sandler; Fiscal Year: 2013
    ..IL-4, IL-17, INF(, IL-8, IL-10 and TGF-(;macrophages, NK cells, T cells- CD4+ and CD8+;protein expression of NF-(B (p65) and STAT3) among subjects with and without adenomas, 3) assess the association between adherent bacteria profiles ..
  41. The role of NF-kB signaling in Aging
    JEREMY SCOTT TILSTRA; Fiscal Year: 2010
    ..Similarly, the role of NF-KB in aging will be analyzed using ERCC1 mutant mice heterozygous for the p65 subunit of NF-KB...
  42. SIMON PATRICK HOGAN; Fiscal Year: 2015
    ..we show that colonic M[unreadable]s express the calprotectin receptor (RAGE);and calprotectin stimulates p65 activation and CCL11 expression in M[unreadable]s in vitro...
  43. Lee Quinton; Fiscal Year: 2016
    ....
  44. Suppression of HIV-1 in CNS by a novel protein from ST John's Wort
    Shohreh Amini; Fiscal Year: 2010
    ..studies have ascribed important roles for several transcription factors such as the C/EBPa family, NFkB (p50/p65), and the viral transactivator, Tat, and the late auxiliary protein, Vpr, in these cells...
  45. NF-kappaBeta Therapy for Duchenne Muscular Dystrophy
    Denis C Guttridge; Fiscal Year: 2012
    ..Genetic ablation of either the transcriptionally competent p65 subunit of NFkB or the IKKbeta subunit, that is sensitive to NBD treatment, modulated the immune response and ..
  46. Christopher K Glass; Fiscal Year: 2016
    ..nuclear receptor Nurr1 is induced in response to TLR signaling and recruits CoREST/LSD1 co-repressor complexes to p65 at NFkB-responsive promoters...
  47. Anti-inflammatory Assay for Cystic Fibrosis
    Rangan Maitra; Fiscal Year: 2010
    ..a Pseudomonas aeruginosa bacterial filtrate (PAF)-induced image-based translocation assay for the NF?B subunit p65 in CF airway epithelial cells...
  48. Adipose Tissue Hypoxia and Inflammation in Obesity
    Jianping Ye; Fiscal Year: 2013
    ..A role of macrophage in angiogenesis will be investigated in adipocyte-specific NF-kB p65 knockout mice...
  49. Khalid Matrougui; Fiscal Year: 2014
    ..Our preliminary data indicate an increase in oxidative stress (superoxide;O2.-), p65- NFkB activity and PARP-1 activity in coronary arterioles from db-/db- mice...
  50. Mycoplasma pneumoniae Gliding Motility
    Duncan C Krause; Fiscal Year: 2012
    ..Aim 1 addresses the gliding mechanism, focusing on gliding-associated proteins P65, P41 and P24. The terminal organelle detaches from the cell body with the loss of P41 but retains gliding function...
  51. Elliott D Kieff; Fiscal Year: 2016
    ..Our discoveries will elucidate new key targets in NFkB activation and more broadly advance priority target-based tool compound discovery. ..
  52. Radiocontrast Nephropathy: Redox Degradation Catalyst and Nitric Oxide Donor
    ZSUZSANNA KINGA ZSENGELLER; Fiscal Year: 2013
    ..pro-inflammatory transcription factor expression (cytoplasmic I[unreadable]B[unreadable] degradation, nuclear p65 translocation), histologic damage, and nuclear damage and DNA repair (PARP activation and nitrosative stress as ..
  53. Nam V Vo; Fiscal Year: 2016
    ..role of NF-?B in mediating the establishment of cellular senescence in discs of mice genetically depleted of the p65 subunit of NF-?B, Aim #2: the metabolic phenotype of senescent disc cells as it relates to PG homeostasis and SASP, ..
  54. Stephanie A Shore; Fiscal Year: 2016
    ..We will use EMSA and Western blotting for I?B, p50, and p65 to determine O3-induced NF-?B activation is increased in obese mice...
  55. Farah D Lubin; Fiscal Year: 2016
    ..AIM 3. NF-[unreadable]B (p65) is a non-histone target of protein lysine methyltransferases, and once methylated NF[unreadable]B can associate ..
  56. Xiaojiang Cui; Fiscal Year: 2015
    ..cells and breast cancer cells increased cell proliferation, migration, invasion, and protein levels of NF-kB p65 and ?-catenin...
  57. The role of CCR5 in HIV-1 Tat and morphine-mediated neurodegeneration.
    Elizabeth M Podhaizer; Fiscal Year: 2013
    ..Maraviroc is also sufficient to reduce glial NF-?B p65 activation and chemokine release, confirming that CCR5 is a critical mediator of glial inflammatory signaling...
  58. MELANIE MARIA OTT; Fiscal Year: 2014
    ..Tat directly interacts with the deacetylase domain of SIRT1 and blocks the ability of SIRT1 to deacetylate the RelA/p65 subunit of NF-B...
  59. Genome-wide protein-DNA interactions responding to chronic opioid treatment
    Yulong Chen; Fiscal Year: 2012
    ..We will establish this method to analyze genome-wide CBP/p300-NF-:B/p65-DNA interactions in mu and delta opioid receptor-expressing cells after acute and chronic morphine (Specific Aim 1) ..
  60. Deborah V Novack; Fiscal Year: 2015
    ..In the first funding period, we determined that the classical NF-?B pathway, mediated by the p65/RelA subunit, is important for OC precursor survival, but not differentiation...
  61. Gutian Xiao; Fiscal Year: 2016
    ..PDLIM2 functions as a ubiquitin E3 ligase to selectively degrade nuclear (activated) NF- ?B RelA (also known as p65), a transcription factor that has been suggested to play a causative role in tumorigenesis and therapeutic ..
  62. Molecular Mechanisms of Campylobacter Jejuni-induced Pathogenesis
    Christian Jobin; Fiscal Year: 2011
    ..jejuni-induced pathogenesis through genetic removal of the NF?B transcriptional subunit RelA (p65) in the susceptible murine strain IL-10-/-...
  63. Finding Small Molecules that Modulate Gene Expression
    Philip H Bolton; Fiscal Year: 2010
    ....
  64. Alexander Hoffmann; Fiscal Year: 2014
    ....
  65. Transformation of Cells by the REL Oncogene
    Thomas Gilmore; Fiscal Year: 2009
    ....
  66. Norbert E Kaminski; Fiscal Year: 2016
    ..cells, TCDD- treatment caused elevated and sustained levels of BCL6, and decreased levels of CD80, CD69, pERK, p- p65/NF?B, and p-cJun, which are hallmarks of impaired B cell activation...
  67. Lianping Xing; Fiscal Year: 2016
    ....
  68. Andrei Korostelev; Fiscal Year: 2016
    ..Our Specific Aim 2 is designed to address mechanistic questions concerning YaeJ-mediated response to ribosome stalling. The proposed aims will be accomplished by structural and biochemical methods. ..
  69. Simultaneous latent HIV reactivation and cytokine gene suppression
    Monty A Montano; Fiscal Year: 2013
    ..At cytokine gene promoters, we hypothesize that JQ1 interferes with acetylated p65/RelA interaction with Brd4 resulting in declines in NF- B driven cytokine gene expression, but not NFAT driven gene ..
  70. Phase I Trial of Bortezomib and Romidepsin in CLL and Small Cell Lymphoma
    Steven Grant; Fiscal Year: 2010
    ..This will position us to perform future trials that will determine the effectiveness of this novel drug combination in patients with CLL or SLL and address the validity of our preclinical pharmacodynamic observations. ..
  71. Preclinical Trials of NFkappaB Inhibition in the Treatment of Muscular Dystrophy
    C Carlson; Fiscal Year: 2009
    ..Preliminary studies showing an increased total cellular expression of p65 in dystrophic (mdx) muscle also indicate the potential clinical utility of agents that decrease the expression of ..
  72. BIOCHEMISTRY OF SYNAPTIC VESICLES FROM BRAIN
    Erik Floor; Fiscal Year: 1991
    ..The question of whether one major synaptic vesicle protein, p65, is a component of the putative pump will be investigated by cross-linking...
  73. Proteasome/HDAC Inhibition in Leukemia/MDS; Phase I Trial and Correlative Studies
    Steven Grant; Fiscal Year: 2010
    ..synergistic manner in cultured and primary AML blasts to induce apoptosis in association with diminished nuclear p65/RelA localization, down-regulation of NF-:B-dependent proteins (Bcl-xL and XIAP), and up- regulation of Bim...
  74. Regulation of lymphocyte survival by NF-kB proteins
    Amer Beg; Fiscal Year: 2004
    ..These studies may also provide insights into development of strategies for enhancing lymphopoiesis under therapeutic settings. ..