NMNAT1

Summary

Gene Symbol: NMNAT1
Description: nicotinamide nucleotide adenylyltransferase 1
Alias: LCA9, NMNAT, PNAT1, nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, NaMN adenylyltransferase 1, nicotinamide mononucleotide adenylyltransferase 1, nicotinate-nucleotide adenylyltransferase 1, pyridine nucleotide adenylyltransferase 1
Species: human

Top Publications

  1. pmc Characterization of nicotinamide mononucleotide adenylyltransferase from thermophilic archaea
    N Raffaelli
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, Universita di Ancona, Italy
    J Bacteriol 179:7718-23. 1997
  2. ncbi Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes
    V Saridakis
    Division of Molecular and Structural Biology, Ontario Cancer Institute, 610 University Avenue Toronto M5G 2M9, Ontario, Canada
    J Biol Chem 276:7225-32. 2001
  3. ncbi Identification of a locus (LCA9) for Leber's congenital amaurosis on chromosome 1p36
    T Jeffrey Keen
    Molecular Medicine Unit, University of Leeds, Leeds, UK
    Eur J Hum Genet 11:420-3. 2003
  4. ncbi Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis
    Leonardo Sorci
    Istituto di Biotecnologie Biochimiche, Universita Politecnica delle Marche, Via Ranieri 67, 60131 Ancona, Italy
    Biochemistry 46:4912-22. 2007
  5. pmc NMNAT1 mutations cause Leber congenital amaurosis
    Marni J Falk
    Department of Pediatrics, Division of Human Genetics, The Children s Hospital of Philadelphia, Pennsylvania, USA
    Nat Genet 44:1040-5. 2012
  6. doi Mutations in NMNAT1 cause Leber congenital amaurosis with early-onset severe macular and optic atrophy
    Isabelle Perrault
    Department of Genetics, Institut National de la Santé et de la Recherche Médicale U, Université Paris Descartes Sorbonne Paris Cité, France
    Nat Genet 44:975-7. 2012
  7. doi Exome sequencing identifies NMNAT1 mutations as a cause of Leber congenital amaurosis
    Pei Wen Chiang
    Casey Eye Institute Molecular Diagnostic Laboratory, Portland, Oregon, USA
    Nat Genet 44:972-4. 2012
  8. ncbi Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms
    Felicitas Berger
    Freie Universitat Berlin, Institut fur Biochemie, Thielallee 63, 14195 Berlin, Germany
    J Biol Chem 280:36334-41. 2005
  9. ncbi The NMN/NaMN adenylyltransferase (NMNAT) protein family
    Corinna Lau
    Department of Molecular Biology, University of Bergen, Thormohlensgate 55, N 5008 Bergen, Norway
    Front Biosci (Landmark Ed) 14:410-31. 2009
  10. pmc Mutations in NMNAT1 cause Leber congenital amaurosis and identify a new disease pathway for retinal degeneration
    Robert K Koenekoop
    McGill Ocular Genetics Laboratory, McGill University Health Centre, Montreal, Quebec, Canada
    Nat Genet 44:1035-9. 2012

Research Grants

  1. WILLIAM L KRAUS; Fiscal Year: 2016
  2. Rafael Galindo; Fiscal Year: 2016
  3. Mechanisms of Neuronal Maintenance and Protection.
    Rong Grace Zhai; Fiscal Year: 2013
  4. Robert Harris; Fiscal Year: 2015
  5. Jeffrey D Milbrandt; Fiscal Year: 2016
  6. Eric A Pierce; Fiscal Year: 2016
  7. C Michael Crowder; Fiscal Year: 2014
  8. RACHEL FYNVOLA TYNDALE; Fiscal Year: 2014
  9. Targeting cofactor biosynthesis in biodefense pathogens
    Andrei Osterman; Fiscal Year: 2009
  10. Mechanisms of Neurodegeneration & Neuroprotection in EAE
    Tanuja Chitnis; Fiscal Year: 2009

Scientific Experts

Detail Information

Publications141 found, 100 shown here

  1. pmc Characterization of nicotinamide mononucleotide adenylyltransferase from thermophilic archaea
    N Raffaelli
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, Universita di Ancona, Italy
    J Bacteriol 179:7718-23. 1997
    ..Molecular and kinetic properties of the enzymes from both archaea are reported and compared with those already known for the mesophilic eukaryotic NMN adenylyltransferase...
  2. ncbi Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes
    V Saridakis
    Division of Molecular and Structural Biology, Ontario Cancer Institute, 610 University Avenue Toronto M5G 2M9, Ontario, Canada
    J Biol Chem 276:7225-32. 2001
    ..In addition, the role of the conserved (16)HXGH(19) active site motif in catalysis was probed by mutagenic, enzymatic and crystallographic techniques, including the characterization of an NMN(+)/SO4(2-) complex of mutant H19A NMNATase...
  3. ncbi Identification of a locus (LCA9) for Leber's congenital amaurosis on chromosome 1p36
    T Jeffrey Keen
    Molecular Medicine Unit, University of Leeds, Leeds, UK
    Eur J Hum Genet 11:420-3. 2003
    ..We have identified a new locus, LCA9, on chromosome 1p36, at which the disease segregates in a single consanguineous Pakistani family...
  4. ncbi Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis
    Leonardo Sorci
    Istituto di Biotecnologie Biochimiche, Universita Politecnica delle Marche, Via Ranieri 67, 60131 Ancona, Italy
    Biochemistry 46:4912-22. 2007
    ..7.7.1). ATP binds before NMN with nuclear isozyme NMNAT1 and Golgi apparatus NMNAT2, but the opposite order is observed with the mitochondrial isozyme NMNAT3...
  5. pmc NMNAT1 mutations cause Leber congenital amaurosis
    Marni J Falk
    Department of Pediatrics, Division of Human Genetics, The Children s Hospital of Philadelphia, Pennsylvania, USA
    Nat Genet 44:1040-5. 2012
    ..Using exome sequencing we identified a homozygous missense mutation (c.25G>A, p.Val9Met) in NMNAT1 that is likely to be disease causing in two siblings of a consanguineous Pakistani kindred affected by LCA...
  6. doi Mutations in NMNAT1 cause Leber congenital amaurosis with early-onset severe macular and optic atrophy
    Isabelle Perrault
    Department of Genetics, Institut National de la Santé et de la Recherche Médicale U, Université Paris Descartes Sorbonne Paris Cité, France
    Nat Genet 44:975-7. 2012
    ..in nicotinamide adenine dinucleotide (NAD(+)) synthesis, the nuclear nicotinamide mononucleotide adenyltransferase NMNAT1 acts as a chaperone that protects against neuronal activity-induced degeneration...
  7. doi Exome sequencing identifies NMNAT1 mutations as a cause of Leber congenital amaurosis
    Pei Wen Chiang
    Casey Eye Institute Molecular Diagnostic Laboratory, Portland, Oregon, USA
    Nat Genet 44:972-4. 2012
    ..507G>A, p.Trp169*) and missense (c.769G>A, p.Glu257Lys) mutations in NMNAT1, which encodes an enzyme in the nicotinamide adenine dinucleotide (NAD) biosynthesis pathway implicated in ..
  8. ncbi Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms
    Felicitas Berger
    Freie Universitat Berlin, Institut fur Biochemie, Thielallee 63, 14195 Berlin, Germany
    J Biol Chem 280:36334-41. 2005
    ..Analyses of the subcellular localization confirmed NMNAT1 to be a nuclear protein, whereas NMNAT2 and -3 were localized to the Golgi complex and the mitochondria, ..
  9. ncbi The NMN/NaMN adenylyltransferase (NMNAT) protein family
    Corinna Lau
    Department of Molecular Biology, University of Bergen, Thormohlensgate 55, N 5008 Bergen, Norway
    Front Biosci (Landmark Ed) 14:410-31. 2009
    ..This article intends to provide a comprehensive overview of the family of NMNATs and highlights some of the recently identified functional roles of these enzymes...
  10. pmc Mutations in NMNAT1 cause Leber congenital amaurosis and identify a new disease pathway for retinal degeneration
    Robert K Koenekoop
    McGill Ocular Genetics Laboratory, McGill University Health Centre, Montreal, Quebec, Canada
    Nat Genet 44:1035-9. 2012
    ..mutations in the nicotinamide adenine dinucleotide (NAD) synthase gene NMNAT1 encoding nicotinamide mononucleotide adenylyltransferase 1 in eight families with LCA, including the family in which LCA was originally linked to the ..
  11. pmc Regulation of poly(ADP-ribose) polymerase 1 activity by the phosphorylation state of the nuclear NAD biosynthetic enzyme NMN adenylyl transferase 1
    Felicitas Berger
    Institute of Biochemistry, Free University Berlin, 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 104:3765-70. 2007
    ..We report here that the nuclear isoform NMNAT-1 stimulates PARP-1 activity and binds to PAR...
  12. ncbi NAD(+) and axon degeneration revisited: Nmnat1 cannot substitute for Wld(S) to delay Wallerian degeneration
    L Conforti
    The Babraham Institute, Cambridge CB2 4AT, UK
    Cell Death Differ 14:116-27. 2007
    The slow Wallerian degeneration protein (Wld(S)), a fusion protein incorporating full-length nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1), delays axon degeneration caused by injury, toxins and genetic mutation...
  13. pmc Wld S requires Nmnat1 enzymatic activity and N16-VCP interactions to suppress Wallerian degeneration
    Michelle A Avery
    Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Biol 184:501-13. 2009
    Slow Wallerian degeneration (Wld(S)) encodes a chimeric Ube4b/nicotinamide mononucleotide adenylyl transferase 1 (Nmnat1) fusion protein that potently suppresses Wallerian degeneration, but the mechanistic action of Wld(S) remains ..
  14. ncbi The Wlds mutation delays robust loss of motor and sensory axons in a genetic model for myelin-related axonopathy
    Mohtashem Samsam
    Department of Neurology, University of Wurzburg, D 97080 Wurzburg, Germany
    J Neurosci 23:2833-9. 2003
    ..because of fusion of the ubiquitination factor E4B (Ube4b) and nicotinamide mononucleotide adenylyltransferase (Nmnat) genes...
  15. ncbi Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growth
    Shin Nosuke Hashida
    Institute of Molecular and Cellular Biosciences IMCB, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Plant J 49:694-703. 2007
    While mammals and fungi possess nicotinate/nicotinamide mononucleotide adenyltransferase (NMNAT) isoforms, Arabidopsis thaliana only contains a single NMNAT gene, AtNMNAT (At5g55810)...
  16. doi Targeting NMNAT1 to axons and synapses transforms its neuroprotective potency in vivo
    Elisabetta Babetto
    The Babraham Institute, Laboratory of Molecular Signalling, Cambridge CB22 3AT, UK
    J Neurosci 30:13291-304. 2010
    ..and other recent reports support a model in which Wld(S) protects by extranuclear redistribution of its nuclear NMNAT1 portion...
  17. ncbi The antitumor drug, 1,3-bis(2-chloroethyl)-1-nitroso-urea, inactivates human nicotinamide mononucleotide adenylyltransferase
    M Emanuelli
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, Universita di Ancona, Italy
    Biochem Pharmacol 49:575-9. 1995
    ..Incubation of BCNU-inactivated and dialysed NMN adenylyltransferase with dithiothreitol results in a partial recovery of the enzymatic activity...
  18. ncbi Analysis of the nourseothricin-resistance gene (nat) of Streptomyces noursei
    H Krügel
    Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of GDR, Jena
    Gene 62:209-17. 1988
    ..Nc) was cloned from the producer Streptomyces noursei into Streptomyces lividans on the vector pIJ702 to form pNAT1. The nat gene was localized on a 1-kb SalI-MboI fragment, which also carries the nat promoter...
  19. pmc Homology modeling and deletion mutants of human nicotinamide mononucleotide adenylyltransferase isozyme 2: new insights on structure and function relationship
    Lucia Brunetti
    Dipartimento di Patologia Molecolare e Terapie Innovative, Sezione di Biochimica, Universita Politecnica delle Marche, 60131 Ancona, Italy
    Protein Sci 19:2440-50. 2010
    ..Humans possess three NMNAT isozymes (NMNAT1, NMNAT2, and NMNAT3) that differ in size and sequence, gene expression pattern, subcellular localization, ..
  20. ncbi Structure and function of nicotinamide mononucleotide adenylyltransferase
    G Magni
    Istituto di Biotecnologie Biochimiche, Universita Politecnica delle Marche, Ancona, Italy
    Curr Med Chem 11:873-85. 2004
    The enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT), a member of the nucleotidyltransferase alpha/beta phosphodiesterase superfamily, catalyzes the reaction NMN + ATP = NAD + PPi, representing the final step in the ..
  21. pmc Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis
    Oleg V Kurnasov
    Integrated Genomics, Inc, Chicago, Illinois 60612, USA
    J Bacteriol 184:6906-17. 2002
    ..transcriptional regulation of NAD biosynthesis, (ii) a central nicotinamide mononucleotide adenylyltransferase (NMNAT) domain, and (iii) a C-terminal RNK domain...
  22. doi Axon degeneration: Mechanisms and implications of a distinct program from cell death
    Tingting Yan
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Chinese Academy of Sciences, Shanghai 200031, China Graduate School of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Neurochem Int 56:529-34. 2010
    ..The mitochondrial Nmnat3 and cytoplasm-localized mutants of Wld(S) and Nmnat1 have similar or even stronger effect than Wld(S) to delay axon degeneration, which suggest that increased ..
  23. ncbi Crystal structure of Haemophilus influenzae NadR protein. A bifunctional enzyme endowed with NMN adenyltransferase and ribosylnicotinimide kinase activities
    S Kumar Singh
    Department of Biochemistry and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 277:33291-9. 2002
    ..NadR protein (hiNadR) has been shown to be a bifunctional enzyme possessing both NMN adenylytransferase (NMNAT; EC ) and ribosylnicotinamide kinase (RNK; EC ) activities...
  24. ncbi Rat liver mitochondria can synthesize nicotinamide adenine dinucleotide from nicotinamide mononucleotide and ATP via a putative matrix nicotinamide mononucleotide adenylyltransferase
    M Barile
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Bari, Italy
    Biochem Mol Biol Int 38:297-306. 1996
    ..C. 2.7.7.1.), inhibited by PPi, AMP and ADP-ribose. Some features of this enzyme, including the substrate, pH and temperature dependence were also investigated...
  25. pmc Low nicotinamide mononucleotide adenylyltransferase activity in a tiazofurin-resistant cell line: effects on NAD metabolism and DNA repair
    S Boulton
    Cancer Research Unit, Medical School, University of Newcastle upon Tyne, UK
    Br J Cancer 76:845-51. 1997
    ..of low cellular NAD levels in a cell line deficient in nicotinamide mononucleotide adenylyltransferase (NMNAT), an enzyme essential for NAD biosynthesis, were investigated by assessing NAD metabolism and DNA repair after ..
  26. ncbi A rat model of slow Wallerian degeneration (WldS) with improved preservation of neuromuscular synapses
    Robert Adalbert
    The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    Eur J Neurosci 21:271-7. 2005
    ..We generated transgenic Wld(S) rats expressing the Ube4b/Nmnat1 chimeric gene in the central and peripheral nervous system...
  27. ncbi NAD+ metabolism in lymphocytes of patients affected by B-cell chronic lymphocytic leukaemia
    V Polzonetti
    Dipartimento di Scienze Morfologiche e Biochimiche Comparate, University di Camerino, Italy
    Int J Immunopathol Pharmacol 17:1-6. 2004
    ..by B-cell chronic lymphocytic leukemia (B-CLL): NADase activity for NAD+ degradation and NMN adenylyltransferase (NMNAT) activity for NAD+ biosynthesis...
  28. pmc Bifunctional NMN adenylyltransferase/ADP-ribose pyrophosphatase: structure and function in bacterial NAD metabolism
    Nian Huang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 16:196-209. 2008
    ..The latter observation provided new insights into the ligand binding mode of ADPR-gated Ca2+ channel TRPM2...
  29. pmc SIRT1-dependent regulation of chromatin and transcription: linking NAD(+) metabolism and signaling to the control of cellular functions
    Tong Zhang
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Biochim Biophys Acta 1804:1666-75. 2010
    ..enzymes in the salvage pathway, nicotinamide phosphoribosyltransferase (NAMPT) and nicotinamide mononucleotide adenylyltransferase 1 (NMNAT-1), have revealed important functions for these enzymes in SIRT1-dependent ..
  30. doi Genomic phenotype of non-cultured pulmonary fibroblasts in idiopathic pulmonary fibrosis
    Margaret C Emblom-Callahan
    George Mason University, Department of Molecular and Microbiology, Manassas, VA 20110, USA
    Genomics 96:134-45. 2010
    ..Specifically, FBXO32, CXCL14, BDKRB1 and NMNAT1 were up-regulated, while RARA and CDKN2D were down-regulated...
  31. doi Transcriptional profiling of the injured sciatic nerve of mice carrying the Wld(S) mutant gene: identification of genes involved in neuroprotection, neuroinflammation, and nerve regeneration
    Benoit Barrette
    Laboratory of Endocrinology and Genomics, Department of Molecular Medicine, Universite Laval, CHUL Research Center, Quebec, Canada
    Brain Behav Immun 24:1254-67. 2010
    ..Notably, the Nmnat1, a transcript contained within the sequence of the Wld(S) gene, was upregulated by five to eightfold in the ..
  32. doi Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration
    Laura Conforti
    The Babraham Institute, Cambridge, UK
    FEBS J 278:2666-79. 2011
    NAD(+) synthesizing enzyme NMNAT1 constitutes most of the sequence of neuroprotective protein Wld(S), which delays axon degeneration by 10-fold...
  33. pmc Exome sequencing of index patients with retinal dystrophies as a tool for molecular diagnosis
    Marta Corton
    Department of Genetics, IIS Fundación Jiménez Díaz, CIBERER, Madrid, Spain
    PLoS ONE 8:e65574. 2013
    ..In this work we explored the use of whole exome sequencing (WES) as a tool for identification of RD mutations, with the aim of assessing its applicability in a diagnostic context...
  34. pmc Axon degeneration is key component of neuronal death in amyloid-β toxicity
    Wilson M Alobuia
    Biology Department, University of Central Arkansas, Conway, AR 72035, United States
    Neurochem Int 63:782-9. 2013
    ..b>Nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1) blocks axon degeneration in a variety of paradigms, but it cannot block neuronal ..
  35. pmc Axonal protection by Nmnat3 overexpression with involvement of autophagy in optic nerve degeneration
    Y Kitaoka
    Department of Ophthalmology, St Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
    Cell Death Dis 4:e860. 2013
    ..Previous studies demonstrated the crucial role of nicotinamide mononucleotide adenylyltransferase (Nmnat) 1, 2, and 3 in axonal protection...
  36. pmc SkpA restrains synaptic terminal growth during development and promotes axonal degeneration following injury
    E J Brace
    Department of Developmental Biology, Washington University School of Medicine, St Louis, Missouri 63110, and
    J Neurosci 34:8398-410. 2014
    ..As in highwire mutants, this axonal protection requires Nmnat. Hence, SkpA is a novel negative regulator of the Wnd-Jnk pathway that functions with Hiw to regulate both ..
  37. pmc Metabolic profiling of alternative NAD biosynthetic routes in mouse tissues
    Valerio Mori
    Department of Clinical Sciences, Section of Biochemistry, Polytechnic University of Marche, Ancona, Italy
    PLoS ONE 9:e113939. 2014
    ..The NMNAT activity, mainly represented by nuclear NMNAT1, appears to be high and nonrate-limiting in all examined tissues, except in blood...
  38. doi A novel missense NMNAT1 mutation identified in a consanguineous family with Leber congenital amaurosis by targeted next generation sequencing
    Ying Deng
    National Center for Birth Defect Monitoring, West China Second University Hospital, Sichuan University, Chengdu, China Laboratory of Molecular Epidemiology for Birth Defect, West China Institute of Women and Children s Health, Sichuan University, Chengdu, China
    Gene 569:104-8. 2015
    ..NMNAT1, which encodes the nicotinamide mononucleotide adenylyltransferase 1, has been recently identified to be one of the LCA-causing genes...
  39. pmc Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants
    Yo Sasaki
    From the Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 290:17228-38. 2015
    ..is an autosomal recessive retinal degeneration condition caused by mutations in the NAD(+) biosynthetic enzyme NMNAT1. This condition leads to early blindness but no other consistent deficits have been reported in patients with ..
  40. pmc Discovery and characterization of novel small-molecule inhibitors targeting nicotinamide phosphoribosyltransferase
    Tian Ying Xu
    Department of Pharmacology
    Sci Rep 5:10043. 2015
    ..These inhibitors specifically bound NAMPT, rather than downstream NMNAT. We provided the first chemical case using cellular thermal shift assay to explain the difference between in vitro ..
  41. pmc Nicotinamide mononucleotide adenylyltransferase displays alternate binding modes for nicotinamide nucleotides
    Roland Pfoh
    Department of Biology, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada
    Acta Crystallogr D Biol Crystallogr 71:2032-9. 2015
    Nicotinamide mononucleotide adenylyltransferase (NMNAT) catalyzes the biosynthesis of NAD(+) and NaAD(+)...
  42. pmc Alternative splicing of Drosophila Nmnat functions as a switch to enhance neuroprotection under stress
    Kai Ruan
    Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    Nat Commun 6:10057. 2015
    Nicotinamide mononucleotide adenylyltransferase (NMNAT) is a conserved enzyme in the NAD synthetic pathway. It has also been identified as an effective and versatile neuroprotective factor...
  43. pmc Impact of Genetic Reduction of NMNAT2 on Chemotherapy-Induced Losses in Cell Viability In Vitro and Peripheral Neuropathy In Vivo
    Richard A Slivicki
    Program in Neuroscience, Indiana University, Bloomington, IN, United States of America
    PLoS ONE 11:e0147620. 2016
    ..levels in dorsal root ganglia relative to wildtype (WT) mice with no change in levels of other NMNAT isoforms (NMNAT1 or NMNAT3), NMNAT enzyme activity (i.e...
  44. pmc Nicotinamide mononucleotide adenylyltransferase promotes hypoxic survival by activating the mitochondrial unfolded protein response
    X R Mao
    Department of Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Cell Death Dis 7:e2113. 2016
    Gain-of-function mutations in the mouse nicotinamide mononucleotide adenylyltransferase type 1 (Nmnat1) produce two remarkable phenotypes: protection against traumatic axonal degeneration and reduced hypoxic brain injury...
  45. doi Probing the Druggability Limits for Enzymes of the NAD Biosynthetic Network in Glioma
    Jyothi Padiadpu
    Department of Biochemistry, IISc, Bangalore 560012, India
    J Chem Inf Model 56:843-53. 2016
    ..titrations, we identify NMPRTase as a potential drug target, while eliminating other possible candidates NMNAT, NAPRTase, and NRK...
  46. doi ADP-ribose-derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling
    Roni H G Wright
    Centre de Regulacio Genomica CRG, Barcelona Institute for Science and Technology, Barcelona E 09003, Spain Universitat Pompeu Fabra, Barcelona E 08003, Spain
    Science 352:1221-5. 2016
    ..The nuclear source of ATP is essential for hormone-induced chromatin remodeling, transcriptional regulation, and cell proliferation. ..
  47. ncbi Identification and characterization of YLR328W, the Saccharomyces cerevisiae structural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme
    M Emanuelli
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, University of Ancona, Italy
    FEBS Lett 455:13-7. 1999
    ..Molecular and kinetic properties of recombinant NMN adenylyltransferase are reported and compared with those already known for the enzyme obtained from different sources...
  48. pmc A local mechanism mediates NAD-dependent protection of axon degeneration
    Jing Wang
    Division of Neuroscience, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 170:349-55. 2005
    ..the nicotinamide adenine dinucleotide (NAD) synthetic enzyme, nicotinamide mononucleotide adenylyltransferase (Nmnat1)...
  49. ncbi Chronic hypoxia in Andeans; are there lessons for neurology at sea level?
    Otto Appenzeller
    New Mexico Health Enhancement and Marathon Clinics Research Foundation, Albuquerque, NM 87122 1424, USA
    J Neurol Sci 247:93-9. 2006
    ..HSP-90 and Nmnat 1, however, were higher in Andean controls in all locations...
  50. ncbi Tracking in the Wlds--the hunting of the SIRT and the luring of the Draper
    Mike Fainzilber
    Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel
    Neuron 50:819-21. 2006
    ..The Wlds protein is a fusion of nicotinamide mononucleotide adenyltransferase-1 (Nmnat1), an essential enzyme in the biosynthesis pathway of nicotinamide adenine dinucleotide (NAD), with the N-terminal ..
  51. pmc Nmnat delays axonal degeneration caused by mitochondrial and oxidative stress
    Craig Press
    Department of Pathology, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 28:4861-71. 2008
    ..in the production of a chimeric protein that contains the full-length coding sequence of nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1), which alone is sufficient for axonal protection in vitro...
  52. pmc The purine nucleosides adenosine and guanosine delay axonal degeneration in vitro
    Craig Press
    Department of Pathology, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri, USA
    J Neurochem 109:595-602. 2009
    ..uncharacterized, but experiments with wld(s) mutant mice led to the discovery that over-expression of NMN adenylyltransferase 1 or treatment with NAD(+) can inhibit axonal degeneration...
  53. pmc Expression of the neuroprotective slow Wallerian degeneration (WldS) gene in non-neuronal tissues
    Thomas M Wishart
    Centre for Integrative Physiology and Euan MacDonald Centre for Motor Neuron Disease Research, University of Edinburgh Medical School, Edinburgh EH89XD, UK
    BMC Neurosci 10:148. 2009
    ..The Wld(S) gene encodes a novel Ube4b-Nmnat1 chimeric protein (Wld(S) protein) that is responsible for conferring the neuroprotective phenotype...
  54. pmc Synthesizing and salvaging NAD: lessons learned from Chlamydomonas reinhardtii
    Huawen Lin
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Genet 6:e1001105. 2010
    ..QS), quinolate phosphoribosyltransferase (QPT), nicotinate/nicotinamide mononucleotide adenylyltransferase (NMNAT), and NAD+ synthetase (NS)...
  55. ncbi NMN/NaMN adenylyltransferase (NMNAT) and NAD kinase (NADK) inhibitors: chemistry and potential therapeutic applications
    R Petrelli
    School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Italy
    Curr Med Chem 18:1973-92. 2011
    ..Herein we focus on two of them: NMN/NaMN adenylyltransferase (NMNAT) and NAD kinase (NADK)...
  56. pmc NMNAT suppresses tau-induced neurodegeneration by promoting clearance of hyperphosphorylated tau oligomers in a Drosophila model of tauopathy
    Yousuf O Ali
    Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Hum Mol Genet 21:237-50. 2012
    ..of a recently identified neuronal maintenance factor, nicotinamide mononucleotide (NAD) adenylyl transferase (NMNAT), a protein that has both NAD synthase and chaperone function...
  57. pmc A novel Drosophila model of nerve injury reveals an essential role of Nmnat in maintaining axonal integrity
    Yanshan Fang
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Biol 22:590-5. 2012
    ..model, we confirmed an axonal protective function of Wld(S) and nicotinamide mononucleotide adenylyltransferase (Nmnat)...
  58. pmc Nicotinamide mononucleotide adenylyltransferase 2 (Nmnat2) regulates axon integrity in the mouse embryo
    Amy N Hicks
    Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston Salem, North Carolina, USA
    PLoS ONE 7:e47869. 2012
    ..g., muscle) are not functionally innervated resulting in perinatal lethality. In addition, neither Nmnat1 nor 3 can compensate for the loss of Nmnat2...
  59. doi Novel NMNAT1 mutations causing Leber congenital amaurosis identified
    A Kaur
    Department of Medical Genetics, Center of Molecular Medicine and Therapeutics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
    Clin Genet 83:33-4. 2013
    b>NMNAT1 mutations cause Leber congenital amaurosis Falk et al. (2012) Nature Genetics 44: 1040-1045 Mutations in NMNAT1 cause Leber congenital amaurosis and identify a new disease pathway for retinal degeneration Koenekoop et al...
  60. pmc Nicotinamide mononucleotide adenylyltransferase maintains active zone structure by stabilizing Bruchpilot
    Shaoyun Zang
    Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    EMBO Rep 14:87-94. 2013
    ..We show that a neuronal maintenance factor, nicotinamide mononucleotide adenylyltransferase (NMNAT), is required for maintaining active zone structural integrity in Drosophila by interacting with the active zone ..
  61. pmc The role of autophagy in Nmnat-mediated protection against hypoxia-induced dendrite degeneration
    Yuhui Wen
    Department of Molecular and Cellular Pharmacology, University of Miami, Miller School of Medicine, Miami, FL 33136, USA
    Mol Cell Neurosci 52:140-51. 2013
    ..Here, we show that the NAD synthase nicotinamide mononucleotide adenylyltransferase (Nmnat) functions endogenously to protect Drosophila class IV dendritic arborization (da) sensory neurons against hypoxia-..
  62. pmc Protection of mouse retinal ganglion cell axons and soma from glaucomatous and ischemic injury by cytoplasmic overexpression of Nmnat1
    Yanli Zhu
    Department of Neurosurgery, Washington University School of Medicine, St Louis, MO 63110, USA
    Invest Ophthalmol Vis Sci 54:25-36. 2013
    ..We undertook the present study to determine whether the Nmnat1 portion of the chimeric protein provides axonal and somatic protection of RGCs in models of ischemia and glaucoma, ..
  63. pmc The Highwire ubiquitin ligase promotes axonal degeneration by tuning levels of Nmnat protein
    Xin Xiong
    Department of Molecular Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Biol 10:e1001440. 2012
    ..by a new downstream target of Hiw: the NAD+ biosynthetic enzyme nicotinamide mononucleotide adenyltransferase (Nmnat), which acts in parallel to a previously known target of Hiw, the Wallenda dileucine zipper kinase (Wnd/DLK) ..
  64. pmc Simultaneous single-sample determination of NMNAT isozyme activities in mouse tissues
    Giuseppe Orsomando
    Department of Clinical Sciences, DISCo, Section of Biochemistry, Polytechnic University of Marche, Ancona, Italy
    PLoS ONE 7:e53271. 2012
    ..Wld(S) is a spontaneous mutation causing overexpression of NMNAT1 as a fusion protein, which protects injured axons through a gain-of-function...
  65. doi Protective effect of nicotinamide on high glucose/palmitate-induced glucolipotoxicity to INS-1 beta cells is attributed to its inhibitory activity to sirtuins
    Soo Jin Lee
    Institute for Medical Sciences, Ajou University School of Medicine, Suwon, Republic of Korea
    Arch Biochem Biophys 535:187-96. 2013
    ..enzymes such as nicotinamide phosphoribosyl transferase (NAMPT) or nicotinamide mononucleotide adenyltransferase (NMNAT) did not augment death. On the other hand, pharmacological inhibition or knockdown of PARP did not affect death...
  66. pmc Long-lasting alterations to DNA methylation and ncRNAs could underlie the effects of fetal alcohol exposure in mice
    Benjamin I Laufer
    Molecular Genetics Unit, Department of Biology, Western University, London, Ontario, N6A 5B7, Canada
    Dis Model Mech 6:977-92. 2013
    ..The results identified 34 genes that are targeted by the deregulated miRNAs. Of these, four (Pten, Nmnat1, Slitrk2 and Otx2) were viewed as being crucial in the context of FASDs given their roles in the brain...
  67. doi Mitigation of gamma-radiation induced abasic sites in genomic DNA by dietary nicotinamide supplementation: metabolic up-regulation of NAD(+) biosynthesis
    Vipen Batra
    Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India Electronic address
    Mutat Res 749:28-38. 2013
    ..activities of nicotinamide phosphoribosyltransferase (NAMPT) and nicotinamide mononucleotide adenylyltransferase (NMNAT) were observed up to 48h post-irradiation in NSD fed irradiated mice...
  68. pmc Maintaining energy homeostasis is an essential component of Wld(S)-mediated axon protection
    Hua Shen
    Hope Center for Neurological Disorders and Department of Neurology, Washington University School of Medicine, 660 S Euclid Avenue, Saint Louis, MO 63110, USA Electronic address
    Neurobiol Dis 59:69-79. 2013
    ..Wld(S) neurons possessed higher than normal nicotinamide mononucleotide adenylyltransferase (NMNAT) activity...
  69. pmc MEK inhibitor U0126 reverses protection of axons from Wallerian degeneration independently of MEK-ERK signaling
    Catherine Evans
    Signalling Programme, The Babraham Institute, Cambridge, Cambridgeshire, United Kingdom
    PLoS ONE 8:e76505. 2013
    Wallerian degeneration is delayed when sufficient levels of proteins with NMNAT activity are maintained within axons after injury...
  70. pmc Insight into molecular and functional properties of NMNAT3 reveals new hints of NAD homeostasis within human mitochondria
    Roberta Felici
    Department of Health Sciences, University of Florence, Florence, Italy
    PLoS ONE 8:e76938. 2013
    Among the enzymes involved in NAD homeostasis, nicotinamide mononucleotide adenylyltransferases (NMNAT1-3) are central to intracellular NAD formation...
  71. ncbi Modulation of cytotoxicity of benzamide riboside by expression of NMN adenylyltransferase
    Joel A Yalowitz
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, MS4053, Indianapolis 46202 5122, USA
    Curr Med Chem 9:749-58. 2002
    ..metabolite, BAD (benzamide adenine dinucleotide), an analogue of NAD by the action of NMN adenylyltransferase (NMNAT)...
  72. ncbi Cloning, expression and characterization of mouse (Mus musculus) nicotinamide 5'-mononucleotide adenylyltransferase-2
    David W Raches
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, USA
    Med Chem 7:718-26. 2011
    ..adenine dinucleotide (NAD+) is synthesized by the action of nicotinamide mononucleotide adenylyltransferase (NMNAT) from NMN and ATP...
  73. pmc WldS but not Nmnat1 protects dopaminergic neurites from MPP+ neurotoxicity
    Jo Ann V Antenor-Dorsey
    Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Mol Neurodegener 7:5. 2012
    ..axonal protection are unclear, although many studies have attributed WldS neuroprotection to the NAD+-synthesizing Nmnat1 portion of the fusion protein...
  74. doi Nmnat2 protects cardiomyocytes from hypertrophy via activation of SIRT6
    Yi Cai
    Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat Sen University, Guangzhou 510006, China
    FEBS Lett 586:866-74. 2012
    ..Nicotinamide mononucleotide adenylyltransferase (Nmnat) is a central enzyme in NAD biosynthesis...
  75. pmc A model of toxic neuropathy in Drosophila reveals a role for MORN4 in promoting axonal degeneration
    Martha R C Bhattacharya
    Department of Developmental Biology, Washington University in St Louis School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 32:5054-61. 2012
    ..degeneration in Drosophila shares molecular execution mechanisms with vertebrates, including inhibition by both NMNAT (nicotinamide mononucleotide adenylyltransferase) expression and loss of wallenda/DLK (dual leucine zipper kinase)...
  76. ncbi Pyridine dinucleotide biosynthesis in archaebacteria: presence of NMN adenylyltransferase in Sulfolobus solfataricus
    N Raffaelli
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, Universita di Ancona, Italy
    FEBS Lett 355:233-6. 1994
    ..4. The Km values for ATP, NMN and nicotinic acid mononucleotide are 0.08 microM, 1.4 microM and 17 microM, respectively. The enzyme shows a remarkable degree of thermophilicity, with an activation energy of 95 kJ/mol...
  77. ncbi Synechocystis sp. slr0787 protein is a novel bifunctional enzyme endowed with both nicotinamide mononucleotide adenylyltransferase and 'Nudix' hydrolase activities
    N Raffaelli
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, University of Ancona, Italy
    FEBS Lett 444:222-6. 1999
    ..Both activities have been characterized and compared to their archaeal counterparts...
  78. ncbi Identification of the archaeal NMN adenylytransferase gene
    N Raffaelli
    Istituto di Biochimica, Facolta di Medicina, Universita di Ancona, Italy
    Mol Cell Biochem 193:99-102. 1999
    ..jannaschii NMN adenylyltransferase gene. As far as we know from literature, this is the first report on the NMN adenylyltransferase gene...
  79. ncbi Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase
    M Emanuelli
    Istituti di Biochimica, Facolta di Medicina e Chirurgia, University of Ancona, Via Ranieri, 60100 Ancona, Italy
    J Biol Chem 276:406-12. 2001
    ..5 mg of recombinant enzyme/liter of culture medium. The molecular and kinetic properties of recombinant human NMN adenylyltransferase provide new directions for investigating metabolic pathways involving this enzyme...
  80. ncbi Structure of human NMN adenylyltransferase. A key nuclear enzyme for NAD homeostasis
    Silvia Garavaglia
    Department of Genetics and Microbiology A Buzzati Traverso, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
    J Biol Chem 277:8524-30. 2002
    Nicotinamide mononucleotide adenylyltransferase (NMNAT), a member of the nucleotidyltransferase alpha/beta-phosphodiesterases superfamily, catalyzes a universal step (NMN + ATP = NAD + PP(i)) in NAD biosynthesis...
  81. ncbi Wallerian degeneration of injured axons and synapses is delayed by a Ube4b/Nmnat chimeric gene
    T G Mack
    Center for Molecular Medicine ZMMK and Institute for Genetics, University of Cologne, Zuelpicher Strasse 47, D 50674 Cologne, Germany
    Nat Neurosci 4:1199-206. 2001
    ..N-terminal fragment of ubiquitination factor E4B (Ube4b) fused to nicotinamide mononucleotide adenylyltransferase (Nmnat), and showed that it confers a dose-dependent block of Wallerian degeneration...
  82. ncbi Crystal structures of E. coli nicotinate mononucleotide adenylyltransferase and its complex with deamido-NAD
    Hong Zhang
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 10:69-79. 2002
    ..The crystal structures of E. coli NaMN adenylyltransferase (NMNAT) and its complex with deamido-NAD (NaAD) revealed that ligand binding causes large conformational changes in ..
  83. ncbi Crystal structure of human nicotinamide mononucleotide adenylyltransferase in complex with NMN
    Erik Werner
    Crystallography Group, Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13092, Berlin, Germany
    FEBS Lett 516:239-44. 2002
    ..involved in intracellular signaling, is catalyzed by the enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT)...
  84. ncbi Metabolism of the novel IMP dehydrogenase inhibitor benzamide riboside
    Walter Jäger
    Institute of Pharmaceutical Chemistry, University of Vienna, Austria
    Curr Med Chem 9:781-6. 2002
    ....
  85. pmc Age-dependent synapse withdrawal at axotomised neuromuscular junctions in Wld(s) mutant and Ube4b/Nmnat transgenic mice
    Thomas H Gillingwater
    Department of Neuroscience, University of Edinburgh, Edinburgh, EH8 9JZ, UK
    J Physiol 543:739-55. 2002
    Axons in Wld(S) mutant mice are protected from Wallerian degeneration by overexpression of a chimeric Ube4b/Nmnat (Wld) gene. Expression of Wld protein was independent of age in these mice...
  86. ncbi Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis
    Xuejun Zhang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:13503-11. 2003
    ..The characterization of the cytosolic human PNAT-3 provided compelling evidence that the final steps of NAD biosynthesis pathways may exist in mammalian cytoplasm and mitochondria, potentially contributing to their NAD/NADP pool...
  87. ncbi Identification and characterization of a second NMN adenylyltransferase gene in Saccharomyces cerevisiae
    Monica Emanuelli
    Facolta di Medicina, Istituto di Biochimica, Universita di Ancona, Via Ranieri, 60100 Ancona, Italy
    Protein Expr Purif 27:357-64. 2003
    The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (NMNAT) (EC 2.7.7.1) catalyzes the transfer of the adenylyl moiety of ATP to NMN to form NAD(+)...
  88. ncbi Stable inheritance of an 85-kb triplication in C57BL/WldS mice
    Weiqian Mi
    Center for Molecular Medicine ZMMK and Institute for Genetics, University of Cologne, Zuelpicher Strasse 47, Germany
    Mutat Res 526:33-7. 2003
    ..The triplication has now been shown to be the causative mutation, acting through an Ube4b/Nmnat chimeric gene, indicating the possibility of Wld(S) preventing axon degeneration in diverse pathologies and ..
  89. pmc Ultrastructural correlates of synapse withdrawal at axotomized neuromuscular junctions in mutant and transgenic mice expressing the Wld gene
    Thomas H Gillingwater
    Department of Neuroscience, University of Edinburgh, UK
    J Anat 203:265-76. 2003
    We carried out an ultrastructural analysis of axotomized synaptic terminals in Wld(s) and Ube4b/Nmnat (Wld) transgenic mice, in which severed distal axons are protected from Wallerian degeneration...
  90. ncbi Dinucleoside polyphosphate NAD analogs as potential NMN adenylyltransferase inhibitors. Synthesis and biological evaluation
    P Franchetti
    Department of Chemical Sciences, University of Camerino, Camerino, Italy
    Nucleosides Nucleotides Nucleic Acids 22:865-8. 2003
    ..Compounds 1 and 2 proved to be selective inhibitors of microbial enzymes...
  91. ncbi Increased nuclear NAD biosynthesis and SIRT1 activation prevent axonal degeneration
    Toshiyuki Araki
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Science 305:1010-3. 2004
    ..of the ubiquitin assembly protein Ufd2a and the nicotinamide adenine dinucleotide (NAD) biosynthetic enzyme Nmnat1. We demonstrate that increased Nmnat activity is responsible for the axon-sparing activity of the Wlds protein...
  92. ncbi The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells
    Javier R Revollo
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:50754-63. 2004
    ..nicotinamide phosphoribosyltransferase (Nampt) and nicotinamide/nicotinic acid mononucleotide adenylyltransferase (Nmnat), and we examined its effects on the transcriptional regulatory function of the mouse Sir2 ortholog, Sir2alpha, in ..
  93. ncbi The cellular distribution of the Wld s chimeric protein and its constituent proteins in the CNS
    C Fang
    CNS Inflammation Group, Southampton Neuroscience Group, School of Biological Sciences, Biomedical Sciences Building, University of Southampton, Southampton SO16 7PX, UK
    Neuroscience 135:1107-18. 2005
    ..and chimeric forms of ubiquitination factor E4 (Ube 4b) and nicotinamide mononucleotide adenylyltransferase-1 (Nmnat-1)...
  94. ncbi Synthesis and biological evaluation of NAD analogs as human pyridine nucleotide adenylyltransferase inhibitors
    Palmarisa Franchetti
    Dipartimento di Scienze Chimiche, Universita di Camerino, Camerino, Italy
    Nucleosides Nucleotides Nucleic Acids 24:477-9. 2005
    ..at the ribose adenylyl moiety, named N-2'-MeAD and Na-2'-MeAD, were synthesized as ligands of pyridine nucleotide (NMN/NaMN) adenylyltransferase (NMNAT). Both dinucleotides resulted selective inhibitors against human NMNAT-3 isoenzyme.
  95. pmc The slow Wallerian degeneration protein, WldS, binds directly to VCP/p97 and partially redistributes it within the nucleus
    Heike Laser
    Institute for Genetics and Center for Molecular Medicine, University of Cologne, D 50674 Cologne, Germany
    Mol Biol Cell 17:1075-84. 2006
    ..The C-terminal region, derived from NAD(+) synthesizing enzyme Nmnat1, is reported to confer neuroprotection in vitro...
  96. ncbi The neuroprotective WldS gene regulates expression of PTTG1 and erythroid differentiation regulator 1-like gene in mice and human cells
    Thomas H Gillingwater
    Centre for Neuroscience Research, University of Edinburgh, 1 George Square, Edinburgh EH8 9JZ, UK
    Hum Mol Genet 15:625-35. 2006
    ..synapses is blocked in Wld(S) mutant mice by expression of an nicotinamide mononucleotide adenylyl transferase 1 (Nmnat-1)/truncated-Ube4b chimeric gene. The protein product of the Wld(S) gene localizes to neuronal nuclei...
  97. ncbi Stimulation of nicotinamide adenine dinucleotide biosynthetic pathways delays axonal degeneration after axotomy
    Yo Sasaki
    Department of Pathology, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 26:8484-91. 2006
    ..Previous studies demonstrated that overexpression of nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1) or exogenous application of nicotinamide adenine dinucleotide (NAD) can protect ..
  98. ncbi Protection of vincristine-induced neuropathy by WldS expression and the independence of the activity of Nmnat1
    Masashi Watanabe
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, N15, W7 Kita ku, Sapporo 060 8638, Japan
    Neurosci Lett 411:228-32. 2007
    ..degeneration protein (WldS), a fusion protein containing amino-terminal E4B and full-length nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1), delays axon degeneration caused by physical damages, toxins and genetic mutations ..
  99. pmc Drosophila NMNAT maintains neural integrity independent of its NAD synthesis activity
    R Grace Zhai
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Biol 4:e416. 2006
    ..slow (Wld(s)) mice overexpress a chimeric protein containing the NAD synthase NMNAT (nicotinamide mononucleotide adenylyltransferase 1) and exhibit a delay in axonal degeneration...

Research Grants17

  1. WILLIAM L KRAUS; Fiscal Year: 2016
    ..by which NAD+ production by the nuclear NAD+ synthase nicotinamide mononucleotide adenylyltransferase-1 (NMNAT-1) regulates PARP-1 enzymatic activity at target gene promoters...
  2. Rafael Galindo; Fiscal Year: 2016
    ..The goal of this project is to test the hypothesis that endogenous and exogenous NMNAT proteins protect developing neurons from the effects of H-I by increasing the calcium buffering capacity of ..
  3. Mechanisms of Neuronal Maintenance and Protection.
    Rong Grace Zhai; Fiscal Year: 2013
    ..Recently, we have identified a neuronal maintenance factor NMNAT in a forward genetic screen in Drosophila...
  4. Robert Harris; Fiscal Year: 2015
    ..Adenine Dinucleotide (TAD) by the enzyme Nicotinamide 5'-Mononucleotide Adenylyltransferase (NMNAT)...
  5. Jeffrey D Milbrandt; Fiscal Year: 2016
    ..Studies of the wlds mutant mouse led to the discovery that the NAD biosynthetic enzyme Nmnat1 can protect axons from a wide variety of insults, including mechanical trauma, loss of trophic support, and ..
  6. Eric A Pierce; Fiscal Year: 2016
    ..In Aim 2, the function of the NMNAT1 and TTC21B genes in the retina, and how mutations in these genes cause retinal degeneration will be investigated...
  7. C Michael Crowder; Fiscal Year: 2014
    ..The NAD biosynthetic enzyme nicotinamide mononucleotide adenylyl transferase 1 (Nmnat1) has been shown to block both axonal degeneration and hypoxic injury in mouse...
  8. RACHEL FYNVOLA TYNDALE; Fiscal Year: 2014
    ..During the past 4 years of PNAT1, we have characterized genetic variants altering nicotine pharmacokinetics as well as pharmacodynamic genetic ..
  9. Targeting cofactor biosynthesis in biodefense pathogens
    Andrei Osterman; Fiscal Year: 2009
    ..Nicotinate Mononucleotide Adenylyltransferase (NMNAT), a key enzyme in NAD biosynthesis, highly conserved in a broad range of bacterial pathogens, was chosen as the ..
  10. Mechanisms of Neurodegeneration & Neuroprotection in EAE
    Tanuja Chitnis; Fiscal Year: 2009
    ..of neuroprotection in this model is currently unknown, however likely relates to the function of the Ube4b/Nmnat chimeric gene (28)...
  11. Crystallographic Analysis of NMN Adenylytransferases
    Hong Zhang; Fiscal Year: 2005
    DESCRIPTION: (provided by applicant) Nicotinamide mononucleotide adenylyltransferase (NMNAT) is an indispensable enzyme in the biosynthesis and salvage of NAD and NADP in all living organisms...
  12. Crystallographic Analysis of GHMP kinases
    Hong Zhang; Fiscal Year: 2005
    ....
  13. Promoting axon regneration and functional recovery after spinal cord injury
    Zhigang He; Fiscal Year: 2010
    ..We expect that these experiments will provide important insights into the therapeutic potential of manipulating mTOR pathway in promoting axon regrowth and functional recovery after spinal cord injury. ..
  14. Signaling mechanisms of axon regeneration by CNS myelin
    Zhigang He; Fiscal Year: 2009
    ..The second aim will investigate the downstream signaling mechanisms mediated by p75, TROY and perhaps other functionally similar molecules. The involvement of Lingo-1 in this receptor complex will be addressed in the third aim. ..
  15. Nogo receptor and its ligands in axon regeneration
    Zhigang He; Fiscal Year: 2008
    ..These animal models will be used in this study to define the role of myelin inhibitors and other intrinsic and extrinsic factors in limiting the regeneration of different axonal tracts in vivo ..
  16. Signaling Mechanisms mediating effects of Semaphorin3A
    Zhigang He; Fiscal Year: 2005
    ....
  17. Semaphorins and Axon Regeneration in Spinal Cord Injury
    Zhigang He; Fiscal Year: 2003
    ....