NEU3

Summary

Gene Symbol: NEU3
Description: neuraminidase 3
Alias: SIAL3, sialidase-3, N-acetyl-alpha-neuraminidase 3, ganglioside sialidase, membrane sialidase, sialidase 3 (membrane sialidase)
Species: human

Top Publications

  1. doi New findings on nuclear gangliosides: overview on metabolism and function
    Robert Ledeen
    Department of Neurology and Neurosciences, New Jersey Medical School, UMDNJ, Newark, NJ 07103, USA
    J Neurochem 116:714-20. 2011
  2. ncbi Cloning, expression, and chromosomal mapping of a human ganglioside sialidase
    T Wada
    Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi, 981 1293, Japan
    Biochem Biophys Res Commun 261:21-7. 1999
  3. ncbi A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains
    Yan Wang
    Division of Biochemistry, Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi 981 1293, Japan
    J Biol Chem 277:26252-9. 2002
  4. pmc Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression
    Yoichiro Kakugawa
    Division of Biochemistry, Research Institute, Department of Surgery, Miyagi Prefectural Cancer Center, Natori, Miyagi 981 1293, Japan
    Proc Natl Acad Sci U S A 99:10718-23. 2002
  5. pmc Plasma-membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signalling
    Kengo Kato
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori 981 1293, Japan
    Biochem J 394:647-56. 2006
  6. ncbi Plasma membrane-associated sialidase is up-regulated in renal cell carcinoma and promotes interleukin-6-induced apoptosis suppression and cell motility
    Seiji Ueno
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, 981 1293, Japan
    J Biol Chem 281:7756-64. 2006
  7. ncbi A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells
    T Wada
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan
    Oncogene 26:2483-90. 2007
  8. doi Insight into substrate recognition and catalysis by the human neuraminidase 3 (NEU3) through molecular modeling and site-directed mutagenesis
    Amgad Albohy
    Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G2G2, Canada
    Glycobiology 20:1127-38. 2010
  9. doi Regulation of plasma-membrane-associated sialidase NEU3 gene by Sp1/Sp3 transcription factors
    Kazunori Yamaguchi
    Miyagi Cancer Center Research Institute, Natori, Japan
    Biochem J 430:107-17. 2010
  10. pmc Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling
    S Kawamura
    Department of Urology, Miyagi Cancer Center, Natori, Japan
    Cell Death Differ 19:170-9. 2012

Research Grants

  1. STRUCTURE OF AN INFLUENZA B NEURAMINIDASE-FAB COMPLEX
    Ming Luo; Fiscal Year: 1993
  2. Role of Sialidase in Pulmonary Host Defenses and Acute Lung Injury
    Alan Cross; Fiscal Year: 2010
  3. PATHOGENESIS OF PNEUMOCYSTIS CARINII PNEUMONIA
    MIERCIO PEREIRA; Fiscal Year: 1999
  4. PLASMALOPSYCHOSINE AS NEUROTROPHIC FACTOR
    Senitiroh Hakomori; Fiscal Year: 2003
  5. SPHINGOSINE DEPENDENT KINASE AND CARCINOGENESIS
    Senitiroh Hakomori; Fiscal Year: 2003
  6. Role of MUC1 in Repair of Injured Airways
    ERIK LILLEHOJ; Fiscal Year: 2006
  7. Carbohydrate-carbohydrate interaction in basic cell biology
    Sen itiroh Hakomori; Fiscal Year: 2006
  8. Transcytosis of Flagellin Across Airway Epithelia
    ERIK LILLEHOJ; Fiscal Year: 2008
  9. Glycosynapses in cancer cell adhesion and signaling
    Sen itiroh Hakomori; Fiscal Year: 2009
  10. Role of Cellular Sialidase in Pathogenesis of HIV-1
    Nicholas Stamatos; Fiscal Year: 2004

Scientific Experts

  • J Kopitz
  • Farah Alghamdi
  • Erik P Lillehoj
  • Sen itiroh Hakomori
  • Taeko Miyagi
  • Nicholas M Stamatos
  • Yutaro Azuma
  • Trisha M Finlay
  • Zhiyun Wang
  • E Monti
  • Elena Odintsova
  • Sadagopan Magesh
  • May M Luke
  • M Hiraiwa
  • Min Zhang
  • Shigeki Katoh
  • Gusheng Wu
  • Kazunori Yamaguchi
  • Kazuhiro Shiozaki
  • Luigi Anastasia
  • Bruno Venerando
  • Guido Tettamanti
  • Massimo Aureli
  • Nadia Papini
  • Cristina Tringali
  • Keiko Hata
  • Tadashi Wada
  • Setsuko Moriya
  • Amgad Albohy
  • Sandro Sonnino
  • Alessandro Prinetti
  • Christopher W Cairo
  • Roberto Bresciani
  • Vanna Chigorno
  • Ikuro Sato
  • Masahiro Hosono
  • Ming Luo
  • Alan Cross
  • Kohta Takahashi
  • MIERCIO PEREIRA
  • Marco Piccoli
  • Edoardo Giacopuzzi
  • Nicoletta Loberto
  • T Wada
  • Koichi Koseki
  • Federica Cirillo
  • Giuseppe Borsani
  • Yan Wang
  • Augusto Preti
  • Rosaria Bassi
  • Cheorl Ho Kim
  • Rea Valaperta
  • Kiyoto Shiga
  • Francesca D'Avila
  • Momo Shiozaki
  • Loredana Dileo
  • Yao Zou
  • Kengo Kato
  • Jeong Heon Ko
  • Alexey V Pshezhetsky
  • Akira Minami
  • Feng Jia
  • Andrea Ghiroldi
  • Marta Manzoni
  • Hiroshi Kobayashi
  • Koji Yamamoto
  • Christopher Lipina
  • K Yamaguchi
  • Dario Bonardi
  • Petros Kingstone Chigwechokha
  • Masaharu Komatsu
  • Hideaki Yamanami
  • Yi Zhang
  • Ki Tae Ha
  • Yujin Natori
  • S Kawamura
  • Volkan Seyrantepe
  • Erika Conforti
  • Fang Ma
  • Alan S Cross
  • Roland Schauer
  • Mahendra S Sandbhor
  • Maiko Miyata
  • Robert Ledeen
  • Dirk Wipfler
  • Yoichiro Kakugawa
  • Ruixiang Blake Zheng
  • Luigi Caimi
  • Shigeru Saijo
  • Chandan Mandal

Detail Information

Publications103 found, 100 shown here

  1. doi New findings on nuclear gangliosides: overview on metabolism and function
    Robert Ledeen
    Department of Neurology and Neurosciences, New Jersey Medical School, UMDNJ, Newark, NJ 07103, USA
    J Neurochem 116:714-20. 2011
    ..The Neu3 isoform of neuraminidase occurs in the inner membrane of the NE and Neu1 in the outer membrane...
  2. ncbi Cloning, expression, and chromosomal mapping of a human ganglioside sialidase
    T Wada
    Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi, 981 1293, Japan
    Biochem Biophys Res Commun 261:21-7. 1999
    Here we report the cDNA sequence of a human ganglioside sialidase. The cDNA was isolated from a human brain cDNA library by screening with a 240 bp probe generated by polymerase chain reaction using primers based on the sequences of rat ..
  3. ncbi A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains
    Yan Wang
    Division of Biochemistry, Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi 981 1293, Japan
    J Biol Chem 277:26252-9. 2002
    The ganglioside-specific sialidase Neu3 has been suggested to play essential roles in regulation of cell surface functions because of its major localization in the plasma membrane and strict substrate preference for gangliosides involved ..
  4. pmc Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression
    Yoichiro Kakugawa
    Division of Biochemistry, Research Institute, Department of Surgery, Miyagi Prefectural Cancer Center, Natori, Miyagi 981 1293, Japan
    Proc Natl Acad Sci U S A 99:10718-23. 2002
    Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing crucial roles in the regulation of cell ..
  5. pmc Plasma-membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signalling
    Kengo Kato
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori 981 1293, Japan
    Biochem J 394:647-56. 2006
    We have found previously that human plasma-membrane-associated sialidase (NEU3), a key glycosidase for ganglioside degradation, was markedly up-regulated in human colon cancers, with an involvement in suppression of apoptosis...
  6. ncbi Plasma membrane-associated sialidase is up-regulated in renal cell carcinoma and promotes interleukin-6-induced apoptosis suppression and cell motility
    Seiji Ueno
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, 981 1293, Japan
    J Biol Chem 281:7756-64. 2006
    Human plasma membrane-associated sialidase (NEU3), specifically hydrolyzing gangliosides, plays crucial roles in the regulation of cell surface functions...
  7. ncbi A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells
    T Wada
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan
    Oncogene 26:2483-90. 2007
    Human plasma membrane-associated sialidase (NEU3), a key enzyme for ganglioside degradation, is markedly upregulated in human cancers, leading to apoptosis suppression...
  8. doi Insight into substrate recognition and catalysis by the human neuraminidase 3 (NEU3) through molecular modeling and site-directed mutagenesis
    Amgad Albohy
    Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G2G2, Canada
    Glycobiology 20:1127-38. 2010
    ..Members of the family, such as NEU2 and NEU1, are cytosolic or lysosomal, while NEU3 and NEU4 are membrane-associated...
  9. doi Regulation of plasma-membrane-associated sialidase NEU3 gene by Sp1/Sp3 transcription factors
    Kazunori Yamaguchi
    Miyagi Cancer Center Research Institute, Natori, Japan
    Biochem J 430:107-17. 2010
    Gene expression of the human plasma membrane-associated sialidase (NEU3), a key enzyme for ganglioside degradation, is relatively high in brain and is modulated in response to many cellular processes, including neuronal cell ..
  10. pmc Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling
    S Kawamura
    Department of Urology, Miyagi Cancer Center, Natori, Japan
    Cell Death Differ 19:170-9. 2012
    ..treatments for androgen-independent prostate cancer, we have investigated plasma membrane-associated sialidase (NEU3), the key enzyme for ganglioside hydrolysis participating in transmembrane signaling...
  11. ncbi Dependence of neurotrophic factor activation of Trk tyrosine kinase receptors on cellular sialidase
    Alicja Woronowicz
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Glycobiology 17:10-24. 2007
    ..Here, we discover a membrane sialidase-controlling mechanism that depends on ligand binding to its receptor to induce enzyme activity which targets ..
  12. doi Inhibition of human neuraminidase 3 (NEU3) by C9-triazole derivatives of 2,3-didehydro-N-acetyl-neuraminic acid
    Yao Zou
    Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
    Bioorg Med Chem Lett 20:7529-33. 2010
    ..of 2,3-didehydro-N-acetyl-neuraminic acid (DANA) and their inhibitory potency for the human neuraminidase 3 (NEU3) enzyme...
  13. ncbi Molecular cloning of mouse ganglioside sialidase and its increased expression in Neuro2a cell differentiation
    T Hasegawa
    Division of Biochemistry, Research Institute, Miyagi Prefectural Cancer Center, 47 1 Nodayama, Medeshima shiode, Natori, Miyagi 981 1293, Japan
    J Biol Chem 275:8007-15. 2000
    ..further the roles and regulation mechanisms of the sialidase in neuronal systems, we have cloned mouse ganglioside sialidase cDNA and observed its expression in Neuro2a cell differentiation...
  14. ncbi Molecular cloning and characterization of a plasma membrane-associated sialidase specific for gangliosides
    T Miyagi
    Division of Biochemistry, Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi, 981 1293, Japan
    J Biol Chem 274:5004-11. 1999
    ..Here we describe the cloning and expression of a cDNA for the ganglioside sialidase, isolated from a bovine brain cDNA library based on the amino acid sequence of the purified enzyme from ..
  15. ncbi High mannose glycans and sialic acid on gp120 regulate binding of mannose-binding lectin (MBL) to HIV type 1
    Melanie L Hart
    Rush Presbyterian St Luke s Medical Center, Department of Immunology Microbiology, Chicago, Illinois 60612, USA
    AIDS Res Hum Retroviruses 18:1311-7. 2002
    ..These studies provide evidence that MBL binds to HIV via high mannose carbohydrates on gp120 and shows that the interaction of MBL with virus is regulated by sialylation...
  16. pmc Gangliosides play an important role in the organization of CD82-enriched microdomains
    Elena Odintsova
    Cancer Research U K Institute for Cancer Studies, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Biochem J 400:315-25. 2006
    ..Furthermore, ectopic expression of the plasma-membrane-bound sialidase Neu3 in mammary epithelial cells also affected stability of the complexes containing CD82: its association with ..
  17. ncbi Expression of NEU3 (plasma membrane-associated sialidase) in clear cell adenocarcinoma of the ovary: its relationship with T factor of pTNM classification
    Hiroyuki Nomura
    Department of Obstetrics and Gynecology, School of Medicine, Keio University, Shinanomachi, Tokyo, Japan
    Oncol Res 16:289-97. 2006
    ..expression of ovarian cancer cells and to evaluate the relationship between plasma membrane-associated sialidase (NEU3) expression and various clinicopathological factors in ovarian clear cell adenocarcinoma patients...
  18. doi Ionizing radiations increase the activity of the cell surface glycohydrolases and the plasma membrane ceramide content
    Massimo Aureli
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Milan, Italy
    Glycoconj J 29:585-97. 2012
    We detected significant levels of β-glucosidase, β-galactosidase, sialidase Neu3 and sphingomyelinase activities associated with the plasma membrane of fibroblasts from normal and Niemann-Pick subjects and of cells from breast, ovary, ..
  19. doi Identification of selective inhibitors for human neuraminidase isoenzymes using C4,C7-modified 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA) analogues
    Yi Zhang
    Alberta Glycomics Center, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
    J Med Chem 56:2948-58. 2013
    ..Guided by our previous studies of human NEU3 inhibitors, we designed a series of C4,C7-modified analogues of 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA)..
  20. ncbi Acidic sialidase activity is aberrant in obese and diabetic mice
    Yujin Natori
    Department of Life and Health Sciences, School of Pharma Sciences, Teikyo University, Tokyo, Japan
    Biol Pharm Bull 36:1027-31. 2013
    Mammalian sialidases (NEU1, NEU2, NEU3 and NEU4) that remove sialic acids from glycoconjugates have been implicated in diverse cellular functions...
  21. doi Phenolic metabolites of benzene induced caspase-dependent cytotoxicities to K562 cells accompanied with decrease in cell surface sialic acids
    Yan Wang
    School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China
    Environ Toxicol 29:1437-51. 2014
    ..Consistently, an increase in expression level of sialidase Neu3 mRNA and a decrease in mRNA level of sialyltransferase ST3GAL3 gene were detected in hydroquione-, catechol-, or 1,..
  22. doi A novel insulin receptor-signaling platform and its link to insulin resistance and type 2 diabetes
    Farah Alghamdi
    Department of Biomedical and Molecular Sciences, Queen s University, Kingston, Ontario K7L 3N6, Canada Electronic address
    Cell Signal 26:1355-68. 2014
    ..Olanzapine, an antipsychotic agent associated with insulin resistance, induced Neu3 sialidase activity in WG544 or 1140F01 human sialidosis fibroblast cells genetically defective in Neu1...
  23. pmc Sialidase NEU3 dynamically associates to different membrane domains specifically modifying their ganglioside pattern and triggering Akt phosphorylation
    Dario Bonardi
    Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy
    PLoS ONE 9:e99405. 2014
    ..Sialidase NEU3 shows high enzymatic specificity toward gangliosides...
  24. doi Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticus
    Petros Kingstone Chigwechokha
    The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan Department of Fisheries, Mzuzu University, Mzuzu, Malawi
    Gene 552:155-64. 2014
    Mammalian Neu3 is a ganglioside specific sialidase. Gangliosides are involved in various physiological events such as cell growth, differentiation and diseases...
  25. pmc Increased sialidase activity in serum of cancer patients: Identification of sialidase and inhibitor activities in human serum
    Keiko Hata
    Division of Cancer Glycosylation Research, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan
    Cancer Sci 106:383-9. 2015
    ..Among the four types identified to date, we previously demonstrated that plasma membrane-associated ganglioside sialidase (NEU3) is markedly upregulated in various human cancers, including examples in the colon and prostate...
  26. doi Phosphatidic acid-mediated activation and translocation to the cell surface of sialidase NEU3, promoting signaling for cell migration
    Kazuhiro Shiozaki
    Faculty of Fisheries and The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Kagoshima, Japan Division of Cancer Glycosylation Research and Division of Cell Recognition Study, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan and Department of Medical Biotechnology and Translational Medicine, University of Milan, Segrate, Milan, Italy
    FASEB J 29:2099-111. 2015
    The plasma membrane-associated sialidase NEU3 plays crucial roles in regulation of transmembrane signaling, and its aberrant up-regulation in various cancers contributes to malignancy...
  27. pmc Potentiation of epidermal growth factor-mediated oncogenic transformation by sialidase NEU3 leading to Src activation
    Koji Yamamoto
    Departments of Cancar Glycosylation Research, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan Division of Cancer Molecular Biology, Graduate School of Medicine, Tohoku University, Sendai, Japan
    PLoS ONE 10:e0120578. 2015
    We previously demonstrated that sialidase NEU3, a key glycosidase for ganglioside degradation, is up-regulated in various human cancers, leading to increased cell invasion, motility and survival of cancer cells possibly through activation ..
  28. doi NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids
    Christopher Lipina
    Division of Cell Signalling and Immunology, Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Cell Signal 27:1742-50. 2015
    The plasma membrane-associated enzyme NEU3 sialidase functions to cleave sialic acid residues from the ganglioside GM3 thereby promoting its degradation, and has been implicated in the modulation of insulin action...
  29. pmc Upregulation of sialidase NEU3 in head and neck squamous cell carcinoma associated with lymph node metastasis
    Kiyoto Shiga
    Department of Otolaryngology Head and Neck Surgery, Iwate Medical University, Morioka, Japan
    Cancer Sci 106:1544-53. 2015
    ..We found plasma membrane-associated sialidase (NEU3) to be significantly upregulated in tumor compared to non-tumor tissues; particularly, an increase in its mRNA ..
  30. pmc Novel biomarker candidates for the diagnosis of ovarian clear cell carcinoma
    Hiroshi Kobayashi
    Department of Obstetrics and Gynecology, Nara Medical University, Kashihara, Nara 634 8522, Japan
    Oncol Lett 10:612-618. 2015
    ..kinase 2; and fibroblast growth factor receptor 2), anti-apoptosis and survival pathways [sialidase 3 (membrane sialidase)], metabolism (γ-glutamyltransferase 1), chemoresistance (napsin A aspartic peptidase, glutathione ..
  31. pmc NEU3 Sialidase Protein Interactors in the Plasma Membrane and in the Endosomes
    Federica Cirillo
    From the Istituto Di Ricovero e Cura a Carattere Scientifico Policlinico San Donato, 20097 San Donato Milanese, Milan and
    J Biol Chem 291:10615-24. 2016
    b>NEU3 sialidase has been shown to be a key player in many physio- and pathological processes, including cell differentiation, cellular response to hypoxic stress, and carcinogenesis...
  32. doi Integrin-mediated cell migration is blocked by inhibitors of human neuraminidase
    Feng Jia
    Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
    Biochim Biophys Acta 1861:1170-9. 2016
    ..Using a scratch assay, we found that exogenously added Neu3 and Neu4 activity altered rates of cell migration...
  33. pmc Role of Sialidase in Long-Term Potentiation at Mossy Fiber-CA3 Synapses and Hippocampus-Dependent Spatial Memory
    Akira Minami
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52 1 Yada, Suruga ku, Shizuoka 422 8526, Japan
    PLoS ONE 11:e0165257. 2016
    ..Sialidase (EC 3.2.1.18), which has 4 isozymes including Neu1, Neu2, Neu3 and Neu4, regulates the sialylation level of glycans by removing sialic acid from sialylglycoconjugate...
  34. doi NEU3 sialidase role in activating HIF-1α in response to chronic hypoxia in cyanotic congenital heart patients
    Marco Piccoli
    Laboratory of Stem Cells for Tissue Engineering, IRCCS Policlinico San Donato, Piazza Malan 2, 20097 San Donato Milanese, Milan, Italy
    Int J Cardiol . 2016
    ..this line, we recently discovered and reported a novel mechanism of HIF activation, which is mediated by sialidase NEU3. Thus, aim of this study was to test whether NEU3 played any role in the cardiac cell response to chronic hypoxia ..
  35. ncbi Chronic ethanol feeding controls the activities of various sialidases by regulating their relative synthetic rates in the rat liver
    Magnus A Azuine
    The Lipid Research Laboratory 151 T, Veterans Affairs Medical Center, Washington, DC 20422, USA
    Metabolism 54:1056-64. 2005
    ..b>Ganglioside sialidase activities in the hepatic subcellular fractions were also determined...
  36. ncbi Plasma membrane production of ceramide from ganglioside GM3 in human fibroblasts
    Rea Valaperta
    Department of Medical Chemistry, Biochemistry and Biotechnology and Center of Excellence on Neurodegenerative Diseases, University of Milan, Via Fratelli Cervi 93, Segrate 20090, Milan, Italy
    FASEB J 20:1227-9. 2006
    ..conditions able to block endocytosis or lysosomal activity, and the overexpression of the plasma membrane ganglioside sialidase Neu3 corresponded to a higher production of ceramide in the plasma membrane...
  37. doi Identification of lysosomal sialidase NEU1 and plasma membrane sialidase NEU3 in human erythrocytes
    Francesca D'Avila
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, F lli Cervi 93, Segrate, Milan 20090, Italy
    J Cell Biochem 114:204-11. 2013
    ..Our results demonstrate that: (a) Both sialidases NEU1 and NEU3 are present on erythrocyte plasma membrane; (b) NEU1 is kept on the plasma membrane in absence of the protective ..
  38. pmc Sialidases on mammalian sperm mediate deciduous sialylation during capacitation
    Fang Ma
    Glycobiology Research and Training Center and Departments of Cellular, University of California, San Diego, La Jolla, California 92093 0687, USA
    J Biol Chem 287:38073-9. 2012
    ..We report the presence of two sialidases (neuraminidases Neu1 and Neu3) on mammalian sperm. These are themselves shed from sperm during capacitation...
  39. ncbi Colon cancer: genomics and apoptotic events
    Charleen Rupnarain
    School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Johannesburg 2050, South Africa
    Biol Chem 385:449-64. 2004
    ..PUMA, 15-LOX-1, ceramide, butyrate, tributyrin and PPARgamma, whereas the molecules which promote colon cancer cell survival are p53 mutants, Bcl-2, Neu3 and COX-2. Cancer therapies aimed at controlling colon cancer are reviewed briefly.
  40. doi Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes)
    Kazuhiro Shiozaki
    Faculty of Fisheries, Kagoshima University, Kagoshima, Japan
    Biochimie 95:280-9. 2013
    Mammalian Neu3 sialidases are involved in various biological processes, such as cell death and differentiation, through desialylation of gangliosides. The enzymatic profile of Neu3 seems to be highly conserved from birds to mammals...
  41. pmc The plasma membrane sialidase NEU3 regulates the malignancy of renal carcinoma cells by controlling β1 integrin internalization and recycling
    Cristina Tringali
    Department of Medical Biotechnology, University of Milan, Segrate, 20090 Milan, Italy
    J Biol Chem 287:42835-45. 2012
    The human plasma membrane sialidase NEU3 is a key enzyme in the catabolism of membrane gangliosides, is crucial in the regulation of cell surface processes, and has been demonstrated to be significantly up-regulated in renal cell ..
  42. doi Polymorphisms and noncardioembolic stroke in three case-control studies
    May M Luke
    Celera, Alameda, CA 94502, USA
    Cerebrovasc Dis 33:80-5. 2012
    ..Gene variants associated with disease could reveal novel mechanisms. We searched for single nucleotide polymorphisms (SNPs) associated with noncardioembolic stroke (nonCES)...
  43. doi Plasma membrane-associated glycohydrolases along differentiation of murine neural stem cells
    Massimo Aureli
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Via Fratelli Cervi 93, 20090 Segrate, Italy
    Neurochem Res 37:1344-54. 2012
    The activities of plasma membrane associated sialidase Neu3, total β-glucosidase, CBE-sensitive β-glucosidase, non-lysosomal β-glucosyl ceramidase GBA2, β-galactosidase, β-hexosaminidase and sphingomyelinase were determined at three ..
  44. doi Mammalian sialidases: physiological and pathological roles in cellular functions
    Taeko Miyagi
    Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan
    Glycobiology 22:880-96. 2012
    ..Four types of mammalian sialidases have been identified and characterized to date, designated as NEU1, NEU2, NEU3 and NEU4...
  45. pmc NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia: NEU1 restrains endothelial cell migration, whereas NEU3 does not
    Alan S Cross
    Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 287:15966-80. 2012
    ..b>NEU3 was detected both in the cytosol and nucleus...
  46. doi MmNEU3 sialidase over-expression in C2C12 myoblasts delays differentiation and induces hypertrophic myotube formation
    Nadia Papini
    Department of Medical Chemistry, University of Milan, 20090 Milan, Italy
    J Cell Biochem 113:2967-78. 2012
    ..In order to support the role of the plasma membrane-associated sialidase NEU3 in skeletal muscle differentiation and to analyse which events of the process are mainly affected by this sialidase,..
  47. pmc Reduced susceptibility to colitis-associated colon carcinogenesis in mice lacking plasma membrane-associated sialidase
    Kazunori Yamaguchi
    Division of Molecular and Cellular Oncology, Miyagi Cancer Center Research Institute, Natori, Japan
    PLoS ONE 7:e41132. 2012
    ..We previously reported that the membrane-associated mammalian sialidase NEU3, which preferentially acts on gangliosides, is involved in cell differentiation, motility, and tumorigenesis...
  48. pmc New insights on the sialidase protein family revealed by a phylogenetic analysis in metazoa
    Edoardo Giacopuzzi
    Department of Biomedical Sciences and Biotechnology, Unit of Biology and Genetics, University of Brescia, Brescia, Italy
    PLoS ONE 7:e44193. 2012
    ..sialidase forms are known in vertebrates: the lysosomal NEU1, the cytosolic NEU2 and the membrane-associated NEU3 and NEU4...
  49. ncbi Lysosomal and plasma membrane ganglioside GM3 sialidases of cultured human fibroblasts. Differentiation by detergents and inhibitors
    H R Schneider-Jakob
    Institut für Pathochemie und Allgemeine Neurochemie, Universitat Heidelberg
    Biol Chem Hoppe Seyler 372:443-50. 1991
    ..both activities were normal in fibroblasts from patients with mucolipidosis IV, previously thought to be a ganglioside sialidase deficiency disorder...
  50. ncbi Role of plasma membrane ganglioside sialidase of human neuroblastoma cells in growth control and differentiation
    J Kopitz
    Institute of Pathochemistry and General Neurochemistry, University of Heidelberg, Germany
    Biochem Biophys Res Commun 199:1188-93. 1994
    ..The results suggest an important role of the ganglioside sialidase of the plasma membrane in the processes of proliferation control and differentiation in this neuronal cell ..
  51. pmc Infectivities of human and other primate lentiviruses are activated by desialylation of the virion surface
    H Hu
    Department of Viral Infection, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    J Virol 70:7462-70. 1996
    ..Thus, the sialylation catalyzed by host cell pathways appeared to reduce the infectivity of human and nonhuman primate lentiviruses. Our results further suggested that desialylation would help increase the titers of HIV-based vectors...
  52. ncbi Protective protein in the bovine lysosomal beta-galactosidase complex
    M Hiraiwa
    Department of Neurosciences, Center for Molecular Genetics, University of California at San Diego, La Jolla 92093 0634, USA
    Biochim Biophys Acta 1341:189-99. 1997
    ..Both beta-galactosidase and cathepsin A activities were labile under the dissociated condition, indicating that it physiologically stabilizes not only beta-galactosidase but also itself by forming the complex...
  53. pmc Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane
    E Monti
    Telethon Institute of Genetics and Medicine TIGEM, San Raffaele Biomedical Science Park, Via Olgettina 58, 20132 Milan, Italy and Department of Biomedical Science and Biotechnology, University of Brescia, Via Valsabbina 19, 25123 Brescia, Italy
    Biochem J 349:343-51. 2000
    ..Here we describe the identification and expression of a human plasma-membrane-associated sialidase, NEU3, isolated starting from an expressed sequence tag (EST) clone...
  54. ncbi Desialylation of extracellular GD1a-neoganglioprotein suggests cell surface orientation of the plasma membrane-bound ganglioside sialidase activity in human neuroblastoma cells
    J Kopitz
    University of Heidelberg, Department of Pathochemistry and Neurochemistry, Im Neuenheimer Feld 220, D 69120, Heidelberg, Germany
    FEBS Lett 491:233-6. 2001
    ..We conclude that the ganglioside sialidase activity is cell surface-orientated and displays an in situ specificity that mirrors enzyme preparations ..
  55. ncbi Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue
    Christian Oehler
    Institute of Pathochemistry and Neurochemistry, University of Heidelberg, Im Neuenheimer Feld 220 221, D 69120 Heidelberg, Germany
    Biol Chem 383:1735-42. 2002
    ..Here we report on the specificity toward gangliosides and susceptibility to certain inhibitors of a ganglioside sialidase solubilized and purified 5100-fold from human brain...
  56. ncbi Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice
    Akinori Sasaki
    Division of Biochemistry, Miyagi Prefectural Cancer Center, Natori, Miyagi 981 1293, Japan
    J Biol Chem 278:27896-902. 2003
    ..Here we demonstrate that mice overexpressing the human ortholog (NEU3) develop diabetic phenotype by 18-22 weeks associated with hyperinsulinemia, islet hyperplasia, and increased beta-..
  57. ncbi Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells
    Jurgen Kopitz
    Institut für Molekulare Pathologie, Klinikum der Ruprecht Karls Universtiät, Im Neuenheimer Feld 220, 69120 Heidelberg, Germany
    Oncogene 22:6277-88. 2003
    ..On the surface of neuroblastoma cells (SK-N-MC), the increased GM1 density, a result of upregulated ganglioside sialidase activity, is a key factor for the switch from proliferation to differentiation...
  58. ncbi Identification and expression of Neu4, a novel murine sialidase
    Elena M Comelli
    The Scripps Research Institute, Department of Molecular Biology, MEM L71, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Gene 321:155-61. 2003
    ..Of the other known murine sialidases, it is most similar to Neu3 (42%)...
  59. ncbi Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family
    E Monti
    Department of Biomedical Science and Biotechnology, University of Brescia, Viale Europa 11, 25123 Brescia, Italy
    Genomics 83:445-53. 2004
    ..unique among mammalian sialidases, shows a high degree of homology for NEU2 and the plasma membrane-associated (NEU3) sialidases. RNA dot-blot analysis showed a low but wide expression pattern, with the highest level in liver...
  60. ncbi Molecular characterization of membrane type and ganglioside-specific sialidase (Neu3) expressed in E. coli
    Ki Tae Ha
    National Research Laboratory for Glycobiology, Ministry of Science and Technology, Korean Government
    Mol Cells 17:267-73. 2004
    Endogenous expression of human membrane type ganglioside sialidase (Neu3) was examined in various cell lines including NB-1, U87MG, SK-MEL-2, SK-N-MC, HepG2, Hep3B, Jurkat, HL-60, K562, ECV304, Hela and MCF-7...
  61. ncbi Neu4, a novel human lysosomal lumen sialidase, confers normal phenotype to sialidosis and galactosialidosis cells
    Volkan Seyrantepe
    Department of Medical Genetics, Sainte Justine Hospital, University of Montreal, 3175 Cote Ste Catherine, Montreal, Quebec H3T 1C5, Canada
    J Biol Chem 279:37021-9. 2004
    ..have been described as follows: lysosomal (Neu1, gene NEU1), cytoplasmic (Neu2, gene NEU2), and plasma membrane (Neu3, gene NEU3)...
  62. ncbi Asymmetric membrane ganglioside sialidase activity specifies axonal fate
    Jorge Santos Da Silva
    Cavalieri Ottolenghi Scientific Institute, University of Turin, 10043 Orbassano, Turin, Italy
    Nat Neurosci 8:606-15. 2005
    ..Here we show that plasma membrane ganglioside sialidase (PMGS) asymmetrically accumulates at the tip of one neurite of the unpolarized rat neuron, inducing actin ..
  63. ncbi Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages
    Nicholas M Stamatos
    Institute of Human Virology, University of Maryland, Baltimore, MD 21201, USA
    FEBS J 272:2545-56. 2005
    ..this study, we demonstrate that three of these sialidases, lysosomal Neu1 and Neu4 and plasma membrane-associated Neu3, are expressed in human monocytes...
  64. ncbi Homology modeling of human sialidase enzymes NEU1, NEU3 and NEU4 based on the crystal structure of NEU2: hints for the design of selective NEU3 inhibitors
    Sadagopan Magesh
    Department of Applied Bioorganic Chemistry, Gifu University, Gifu 501 1193, Japan
    J Mol Graph Model 25:196-207. 2006
    ..according to their major intracellular location as intralysomal (NEU1), cytosolic (NEU2), plasma membrane (NEU3) and lysosomal or mitochondrial membrane (NEU4) associated sialidases...
  65. ncbi Epidermal growth factor-induced mobilization of a ganglioside-specific sialidase (NEU3) to membrane ruffles
    Kazunori Yamaguchi
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, Japan
    Biochem Biophys Res Commun 346:484-90. 2006
    Human ganglioside-specific sialidase, NEU3, localized at cell membranes is thought to regulate various biological processes at cell surfaces...
  66. ncbi Monocyte differentiation up-regulates the expression of the lysosomal sialidase, Neu1, and triggers its targeting to the plasma membrane via major histocompatibility complex class II-positive compartments
    Feng Liang
    Sainte Justine Hospital and the Department of Pediatrics, University of Montreal, Montreal, Quebec H3T 1C5, Canada
    J Biol Chem 281:27526-38. 2006
    ..In contrast to other cellular sialidases Neu2, Neu3, and Neu4, whose expression either remains unchanged or is down-regulated, Neu1 mRNA, protein and activity are ..
  67. ncbi Sialidase expression in activated human T lymphocytes influences production of IFN-gamma
    Xinli Nan
    Institute of Human Biology, and Department of Medicine, University of Maryland Medical Center, 725 West Lombard Street, Baltimore, MD 21201, USA
    J Leukoc Biol 81:284-96. 2007
    ..In this study, we demonstrate that only lysosomal Neu1 and plasma membrane-associated Neu3 are detected in freshly isolated and activated human T lymphocytes...
  68. ncbi Modification of sialidase levels and sialoglycoconjugate pattern during erythroid and erytroleukemic cell differentiation
    Cristina Tringali
    Department of Medical Chemistry, Biochemistry and Biotechnology, Faculty of Exercise Science, University of Milan, LITA Segrate, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy
    Glycoconj J 24:67-79. 2007
    ..Herein we show that only the lysosomal sialidase Neu1 and the plasma membrane-associated sialidase Neu3 are expressed in CFU-E erythroid precursors and K562 erythroleukemic cells...
  69. ncbi Induction of axonal differentiation by silencing plasma membrane-associated sialidase Neu3 in neuroblastoma cells
    Rea Valaperta
    Department of Medical Chemistry, Biochemistry and Biotechnology, and Center of Excellence on Neurodegenerative Diseases, University of Milan, Segrate, Italy
    J Neurochem 100:708-19. 2007
    A reduction of 70% of the plasma membrane-associated sialidase Neu3 activity, due to a corresponding reduction of the enzyme expression by transducing cells with a short hairpin RNA encoding a sequence target (complementary messenger of ..
  70. ncbi Down-regulation of sialidase NEU4 may contribute to invasive properties of human colon cancers
    Hideaki Yamanami
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Miyagi Cancer Center, 47 1 Nodayama, Medeshima shiode, Natori, Miyagi, 981 1293, Japan
    Cancer Sci 98:299-307. 2007
    ..We previously demonstrated that a human sialidase associated with plasma membranes (NEU3) is up-regulated in human colon cancer and is involved in suppression of apoptosis...
  71. ncbi Overexpression of membrane sialic acid-specific sialidase Neu3 inhibits matrix metalloproteinase-9 expression in vascular smooth muscle cells
    Sung Kwon Moon
    Molecular and Cellular Glycobiology Unit, Department of Biological Science, Sungkyunkwan University, Chunchun Dong 300, Suwon City, Kyunggi Do 440 746, Republic of Korea
    Biochem Biophys Res Commun 356:542-7. 2007
    The ganglioside-specific sialidase Neu3 has been suggested to participate in cell growth, migration, and differentiation...
  72. pmc Sialidase NEU3 is a peripheral membrane protein localized on the cell surface and in endosomal structures
    Gabriele Zanchetti
    Department of Biomedical Sciences and Biotechnology, School of Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy
    Biochem J 408:211-9. 2007
    Sialidase NEU3 is also known as the plasma-membrane-associated form of mammalian sialidases, exhibiting a high substrate specificity towards gangliosides...
  73. pmc Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio)
    Marta Manzoni
    Department of Biomedical Sciences and Biotechnology, School of Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy
    Biochem J 408:395-406. 2007
    ..genes are organized in clusters located on chromosome 21 and are all more closely related to mammalian sialidase NEU3. They were thus named neu3.1, neu3.2, neu3.3, neu3.4 and neu3.5...
  74. ncbi Enhanced expression of membrane-associated sialidase Neu3 decreases GD3 and increases GM3 on the surface of Jurkat cells during etoposide-induced apoptosis
    Yutaro Azuma
    Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274 8510, Japan
    Biol Pharm Bull 30:1680-4. 2007
    ..N-acetylneuraminic acid and measured the mRNA expression of the plasma membrane-associated sialidase Neu3 and the lysozomal Neu1 using real-time PCR...
  75. ncbi Roles of plasma membrane-associated sialidase NEU3 in human cancers
    Taeko Miyagi
    Biochemistry Division, Miyagi Cancer Center Research Institute, 47 1 Nodayama, Medeshima shiode, Natori, Miyagi, 981 1293, Japan
    Biochim Biophys Acta 1780:532-7. 2008
    ..Among human sialidases so far identified, sialidase NEU3 is a key enzyme for ganglioside degradation because of its uniqueness both in its localization in the plasma ..
  76. ncbi Over-expression of mammalian sialidase NEU3 reduces Newcastle disease virus entry and propagation in COS7 cells
    Luigi Anastasia
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Milan, Italy
    Biochim Biophys Acta 1780:504-12. 2008
    ..pattern determined by over-expression of the mammalian plasma-membrane associated, ganglioside specific, sialidase NEU3 would affect the virus-host cell interactions...
  77. doi Membrane type sialidase inhibits the megakaryocytic differentiation of human leukemia K562 cells
    Un Ho Jin
    Molecular and Cellular Glycobiology Unit, Department of Biological Science, Sungkyunkwan University, Chunchun Dong 300, Suwon, Kyunggi Do 440 746, South Korea
    Biochim Biophys Acta 1780:757-63. 2008
    The membrane type sialidase (Neu3) has been suggested to participate in cell growth, migration and differentiation...
  78. doi Plasma membrane-associated sialidase as a crucial regulator of transmembrane signalling
    Taeko Miyagi
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, 981 1293, and CREST, JST, Japan
    J Biochem 144:279-85. 2008
    ..Among those forms identified to date, plasma membrane-associated sialidase, Neu3, is a key enzyme in degradation of gangliosides, for which it exhibits a special substrate preference...
  79. doi Human sialidase as a cancer marker
    Taeko Miyagi
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan
    Proteomics 8:3303-11. 2008
    ..Immunohistochemistry of cancer tissues using the antibody against the plasma membrane sialidase was useful for clinical diagnosis...
  80. doi Disialoganglioside GD3 synthase expression recruits membrane transglutaminase 2 during erythroid differentiation of the human chronic myelogenous leukemia K562 cells
    Sung Koo Kang
    Molecular and Cellular Glycobiology Unit, Department of Biological Science, Sungkyunkwan University, Suwon, Kyunggi Do, Korea
    Proteomics 8:3317-28. 2008
    ..level was increased in GD3 synthase-transfected cells compared with vector- and plasma membrane-associated ganglioside sialidase (Neu3)-transfected cells...
  81. pmc Limited inhibitory effects of oseltamivir and zanamivir on human sialidases
    Keiko Hata
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori 981 1293, Japan
    Antimicrob Agents Chemother 52:3484-91. 2008
    ..the activities of any of the sialidases, even at 1 mM, while zanamivir significantly inhibited the human sialidases NEU3 and NEU2 in the micromolar range (K(i), 3.7 +/- 0.48 and 12.9 +/- 0...
  82. doi Protein sialylation by sialyltransferase involves radiation resistance
    Minyoung Lee
    Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, Seoul, Korea
    Mol Cancer Res 6:1316-25. 2008
    ..In conclusion, radiation exposure was found to increase the sialylation of glycoproteins such as integrin beta1 by inducing the expression of ST6Gal I, and increased protein sialylation contributed to cellular radiation resistance...
  83. doi Silencing of membrane-associated sialidase Neu3 diminishes apoptosis resistance and triggers megakaryocytic differentiation of chronic myeloid leukemic cells K562 through the increase of ganglioside GM3
    C Tringali
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Segrate, Milan, Italy
    Cell Death Differ 16:164-74. 2009
    ..is also blocked because of low levels of ganglioside GM3, derived by the high expression of sialidase Neu3 active on GM3...
  84. pmc NEU3 sialidase strictly modulates GM3 levels in skeletal myoblasts C2C12 thus favoring their differentiation and protecting them from apoptosis
    Luigi Anastasia
    Department of Medical Chemistry, Biochemistry, and Biotechnology, University of Milan, 20090 Milan, Italy
    J Biol Chem 283:36265-71. 2008
    Membrane-bound sialidase NEU3, often referred to as the "ganglioside sialidase," has a critical regulatory function on the sialoglycosphingolipid pattern of the cell membrane, with an anti-apoptotic function, especially in cancer cells...
  85. doi Plasma membrane-associated sialidase (NEU3) promotes formation of colonic aberrant crypt foci in azoxymethane-treated transgenic mice
    Kazuhiro Shiozaki
    Division of Biochemistry, Miyagi Cancer Center Research Institute, 47 1 Nodayama, Medeshima shiode, Natori, Miyagi, 981 1293, Japan
    Cancer Sci 100:588-94. 2009
    Human plasma membrane-associated sialidase (NEU3) specifically hydrolyzes gangliosides, and it is up-regulated in colon cancer and plays an essential role in the expression of malignant phenotypes...
  86. pmc Developmental change of sialidase neu4 expression in murine brain and its involvement in the regulation of neuronal cell differentiation
    Kazuhiro Shiozaki
    Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, Miyagi 981 1293, Japan
    J Biol Chem 284:21157-64. 2009
    ..We previously demonstrated that sialidase Neu3, also rich in mouse brain, is up-regulated during neuronal differentiation with involvement in acceleration of ..
  87. doi Activity of plasma membrane beta-galactosidase and beta-glucosidase
    Massimo Aureli
    Department of Medical Chemistry, Biochemistry and Biotechnology, Center of Excellence on Neurodegenerative Diseases, University of Milano, Segrate, Italy
    FEBS Lett 583:2469-73. 2009
    Human fibroblasts produce ceramide from sialyllactosylceramide on the plasma membranes. Sialidase Neu3 is known to be plasma membrane associated, while only indirect data suggest the plasma membrane association of beta-galactosidase and ..
  88. doi Down regulation of membrane-bound Neu3 constitutes a new potential marker for childhood acute lymphoblastic leukemia and induces apoptosis suppression of neoplastic cells
    Chandan Mandal
    Indian Institute of Chemical Biology, Kolkata, India
    Int J Cancer 126:337-49. 2010
    Membrane-linked sialidase Neu3 is a key enzyme for the extralysosomal catabolism of gangliosides. In this respect, it regulates pivotal cell surface events, including trans-membrane signaling, and plays an essential role in carcinogenesis...
  89. pmc Moderate strength (0.23-0.28 T) static magnetic fields (SMF) modulate signaling and differentiation in human embryonic cells
    Zhiyun Wang
    Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD, USA
    BMC Genomics 10:356. 2009
    ..This study begins to bridge this gulf by investigating static magnetic fields (SMF) through global mRNA profiling in human embryonic cells coupled with software analysis to identify the affected signaling pathways...
  90. doi Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a
    Jianfeng Wang
    Department of Neurology and Neurosciences, New Jersey Medical School, UMDNJ, Newark, New Jersey 07103, USA
    J Neurochem 111:547-54. 2009
    ..Western blot analysis of the separated membranes revealed occurrence of Neu3 in the inner membrane and Neu1 in the outer membrane of the NE...
  91. doi Enhancing glycoprotein sialylation by targeted gene silencing in mammalian cells
    Min Zhang
    Cell Sciences and Development, SAFC Biosciences a member of Sigma Aldrich Group, 2909 Laclede Avenue, Saint Louis, MO 63103, USA
    Biotechnol Bioeng 105:1094-105. 2010
    ..RNA (siRNA) and short-hairpin RNA (shRNA) to reduce expression of two newly identified sialidase genes, Neu1 and Neu3. By knocking down expression of Neu3 we achieved a 98% reduction in sialidase function in CHO cells...
  92. doi Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Galphai proteins and matrix metalloproteinase-9
    Trisha M Finlay
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, K7L3N6, Canada
    Glycoconj J 27:329-48. 2010
    ..RT-PCR and western blot analyses reveal no correlation between mRNA and protein values for Neu3 and Neu4 in human monocytic THP-1 cells, suggesting for the first time a varied post-transcriptional mechanism for ..
  93. pmc A crucial role of sialidase Neu1 in hyaluronan receptor function of CD44 in T helper type 2-mediated airway inflammation of murine acute asthmatic model
    S Katoh
    Department of Cell Regulation, Faculty of Medicine, Kagawa University, Kanagawa, Japan
    Clin Exp Immunol 161:233-41. 2010
    ..Splenic CD4+ T cell binding of HA was examined with flow cytometry. Expression of sialidases (Neu1, Neu2, Neu3 and Neu4) in spleen cells was evaluated by quantitative real-time reverse transcription-polymerase chain reaction...
  94. pmc LPS-induced cytokine production in human dendritic cells is regulated by sialidase activity
    Nicholas M Stamatos
    University of Maryland Medical Center, 725 West Lombard St, Baltimore, MD 21201, USA
    J Leukoc Biol 88:1227-39. 2010
    ..Of the four genetically distinct sialidases (Neu1-4), Neu1, Neu3, and Neu4 are expressed in human monocytes, but only Neu1 and Neu3 are up-regulated as cells differentiate into DCs...
  95. doi Sialidases in vertebrates: a family of enzymes tailored for several cell functions
    Eugenio Monti
    Department of Biomedical Science and Biotechnology, University of Brescia, Italy
    Adv Carbohydr Chem Biochem 64:403-79. 2010
    ..they are the lysosomal (NEU1), the cytosolic (NEU2), and plasma membrane- and intracellular-associated sialidases (NEU3 and NEU4). The molecular cloning of several mammalian sialidases since 1993 has boosted research in this field...
  96. doi Cell surface sphingolipid glycohydrolases in neuronal differentiation and aging in culture
    Massimo Aureli
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Milano, Italy
    J Neurochem 116:891-9. 2011
    ..I, II and IV, sialidase, β-galactosidase and β-glucosidase, and the PM-associated activities of sialidase Neu3, β-galactosidase, Conduritol B Epoxide-sensitive β-glucosidase and β-glucosidase GBA2 in rat cerebellar granule ..
  97. doi Inhibitor selectivity of a new class of oseltamivir analogs against viral neuraminidase over human neuraminidase enzymes
    Amgad Albohy
    Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2
    Bioorg Med Chem 19:2817-22. 2011
    ..influenza group-1 neuraminidase enzyme (N1 subtype) against recombinant forms of the human neuraminidase enzymes NEU3 and NEU4...
  98. doi Differentially regulated expression of 9-O-acetyl GD3 (CD60b) and 7-O-acetyl-GD3 (CD60c) during differentiation and maturation of human T and B lymphocytes
    Dirk Wipfler
    German Cancer Research Center, D015 Translational Immunology, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Glycobiology 21:1161-72. 2011
    ..the key enzyme for GD3 synthesis, was highest in tonsillar T cells, whereas transcriptional levels of sialidase NEU3 and O-acetylesterase H-Lse were lowest in activated T cells...
  99. doi Substrate recognition of the membrane-associated sialidase NEU3 requires a hydrophobic aglycone
    Mahendra S Sandbhor
    Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
    Biochemistry 50:6753-62. 2011
    ..The NEU3 isoform is a membrane-associated enzyme that cleaves glycolipid substrates...
  100. pmc Gallus gallus NEU3 sialidase as model to study protein evolution mechanism based on rapid evolving loops
    Edoardo Giacopuzzi
    Department of Biomedical Sciences and Biotechnology, Unit of Biology and Genetics, University of Brescia, Viale Europa 11, Brescia 25123, Italy
    BMC Biochem 12:45. 2011
    ..These features prompted us to extend our studies on this protein family in birds, to get insights on the evolution of this class of glycohydrolases...
  101. doi Membrane sialidase NEU3 is highly expressed in human melanoma cells promoting cell growth with minimal changes in the composition of gangliosides
    Maiko Miyata
    Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, Aichi, Japan
    Cancer Sci 102:2139-49. 2011
    NEU3 is a membrane sialidase specific for gangliosides. Its increased expression and implication in some cancers have been reported. Here, we analyzed NEU3 expression in malignant melanoma cell lines and its roles in the cancer phenotypes...

Research Grants10

  1. STRUCTURE OF AN INFLUENZA B NEURAMINIDASE-FAB COMPLEX
    Ming Luo; Fiscal Year: 1993
    ..These experiments will define precisely the structure of an epitope on type B influenza virus neuraminidase and the corresponding binding site on the antibody molecule...
  2. Role of Sialidase in Pulmonary Host Defenses and Acute Lung Injury
    Alan Cross; Fiscal Year: 2010
    ..hypothesis, we propose the following: In Specific Aim 1 we will define which form(s) of PMN sialidase(s) (neul and neu3) regulates adherence to and migration across human lung microvascular EC by overexpression and knockdown of ..
  3. PATHOGENESIS OF PNEUMOCYSTIS CARINII PNEUMONIA
    MIERCIO PEREIRA; Fiscal Year: 1999
    ..carinii attachment to lung epithelium; 7) Determine trans- sialidase pathway in P. carinii adherence; 8) Test effectiveness of humoral response to the trans- sialidase against P. carinii infection. ..
  4. PLASMALOPSYCHOSINE AS NEUROTROPHIC FACTOR
    Senitiroh Hakomori; Fiscal Year: 2003
    ..Results of these studies may provide a basis for development of PLPS and its analogues as therapeutic reagents for neurodegenerative diseases and neuronal injury. ..
  5. SPHINGOSINE DEPENDENT KINASE AND CARCINOGENESIS
    Senitiroh Hakomori; Fiscal Year: 2003
    ..In the inhibitory effect is confirmed, its mechanism will be studied in relation to activities of SDKs, MAPK, bcl-2, and other signal transducers. ..
  6. Role of MUC1 in Repair of Injured Airways
    ERIK LILLEHOJ; Fiscal Year: 2006
    ..We expect that future therapies for respiratory diseases, particularly those related to toxicant inhalation, will be developed based upon clinical modulation of MUC1 expression. [unreadable] [unreadable]..
  7. Carbohydrate-carbohydrate interaction in basic cell biology
    Sen itiroh Hakomori; Fiscal Year: 2006
    ..abstract_text> ..
  8. Transcytosis of Flagellin Across Airway Epithelia
    ERIK LILLEHOJ; Fiscal Year: 2008
    ..Based on previous studies of ourselvesand others, we suggest that a major bacterial component (flagellin) is processed differently in CF lungs, leading to enhanced inflammatory responses. ..
  9. Glycosynapses in cancer cell adhesion and signaling
    Sen itiroh Hakomori; Fiscal Year: 2009
    ....
  10. Role of Cellular Sialidase in Pathogenesis of HIV-1
    Nicholas Stamatos; Fiscal Year: 2004
    ..abstract_text> ..