NEK6

Summary

Gene Symbol: NEK6
Description: NIMA related kinase 6
Alias: SID6-1512, serine/threonine-protein kinase Nek6, NIMA (never in mitosis gene a)-related kinase 6, never in mitosis A-related kinase 6, nimA-related protein kinase 6, protein kinase SID6-1512
Species: human

Top Publications

  1. ncbi A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases
    Christopher Belham
    Department of Molecular Biology and Medical Services, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 278:34897-909. 2003
  2. pmc Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2
    T Kobayashi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 339:319-28. 1999
  3. ncbi Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients
    Jian Chen
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China
    Biochem Biophys Res Commun 341:1059-65. 2006
  4. doi Clinical and biological significance of never in mitosis gene A-related kinase 6 (NEK6) expression in hepatic cell cancer
    Xiaolei Cao
    Department of Pathology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People s Republic of China
    Pathol Oncol Res 18:201-7. 2012
  5. ncbi Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo
    Jose M Lizcano
    Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:27839-49. 2002
  6. ncbi Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase
    Yoshihiro Hashimoto
    Department of Biochemistry, Nagoya City University Medical School, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya 467 8601, Japan
    Biochem Biophys Res Commun 293:753-8. 2002
  7. pmc Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression
    Joan Roig
    Department of Molecular Biology and the Diabetes Unit and Medical Services, Massachusetts General Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 16:1640-58. 2002
  8. ncbi Nek6 overexpression antagonizes p53-induced senescence in human cancer cells
    Hye Jin Jee
    Department of Biochemistry, College of Medicine, Dong A University, Busan, South Korea
    Cell Cycle 9:4703-10. 2010
  9. doi Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome
    Gabriela Vaz Meirelles
    Laboratório Nacional de Biociências, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil
    J Proteome Res 9:6298-316. 2010
  10. ncbi Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins
    Eun Jeoung Lee
    School of Science Education, Chungbuk National University, Gaeshin dong, Heungdok gu, Chongju, Chungbuk 361 763, Republic of Korea
    Biochem Biophys Res Commun 358:783-8. 2007

Scientific Experts

  • Andrew M Fry
  • Sixin Liu
  • Lynne Quarmby
  • Hiroyasu Motose
  • Michelle L McCully
  • Satoru Wada
  • Gustavo Delhon
  • Fahd Al-Mulla
  • T Kobayashi
  • D J Templeton
  • Min Jean Yin
  • Jose M Lizcano
  • Jürgen F Kun
  • JANETTA M GARRETT
  • Li Duo-Chuan
  • Hye Jin Jee
  • Laura O'regan
  • Joan Roig
  • Laura Regué
  • Jeanho Yun
  • Hyun Ju Kim
  • Ae Jeong Kim
  • Elmas Kasap
  • Emre Gerçeker
  • Sara Sdelci
  • Minjee Kim
  • Naree Song
  • Mark W Richards
  • M Teresa Bertran
  • Carme Caelles
  • Yugo Hirai
  • Hakan Yuceyar
  • Seda Orenay Boyacıoglu
  • Mehmet Korkmaz
  • Semin Ayhan
  • Shogo Takatani
  • John Yochem
  • Zhouyan Bian
  • Pablo Gallego
  • Mikhail Skoblov
  • David Reverter
  • Richard Bayliss
  • Xiaolei Cao
  • Joseph Avruch
  • Yuese Ning
  • Bo Zhang
  • Gabriela V Meirelles
  • Sunghwan Kim
  • Yan Zhang
  • Jörg Kobarg
  • H Salem
  • Daniel G Hayward
  • Rounak Nassirpour
  • Young Jin Jeon
  • Gabriela Vaz Meirelles
  • Ying Yan
  • Christopher Belham
  • Tatsuya Sakai
  • A Takeno
  • Mukesh Jain
  • Jingyu Xu
  • Min Young Lee
  • Joseph Rapley
  • Duo Chuan Li
  • Eun Jeoung Lee
  • Jian Chen
  • B Motro
  • Lily Q Dong
  • Rashmi Sharma
  • Masuko Katoh
  • Miki Tamura
  • Shigeru Minoguchi
  • Hatice Yıldırm
  • Junji Otani
  • Fuyuki Ishikawa
  • Taku Takahashi
  • Josephina Sampson
  • Daniel Roth
  • Ahmed Baykan
  • Hatice Yildirim
  • Leslie Bell
  • Kento Otani
  • Ender Ellidokuz
  • Axel Knebel
  • Vladimir Lazetic
  • Lihsia Chen
  • DAVID FAY
  • Anne Straube
  • Mai Kanazawa
  • Fiona E Hood

Detail Information

Publications85

  1. ncbi A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases
    Christopher Belham
    Department of Molecular Biology and Medical Services, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 278:34897-909. 2003
    ..b>Nek6 (314AA) and Nek7 (303AA), 76% identical, have little noncatalytic sequence but bind to the carboxyl-terminal ..
  2. pmc Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2
    T Kobayashi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 339:319-28. 1999
    ..These findings raise the possibility that some physiological roles ascribed to PKB on the basis of the overexpression of constitutively active PKB mutants might be mediated by SGK...
  3. ncbi Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients
    Jian Chen
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China
    Biochem Biophys Res Commun 341:1059-65. 2006
    ..In this report, we found that Pin1 could interact with Nek6, one of the human NIMA-related kinases (Neks)...
  4. doi Clinical and biological significance of never in mitosis gene A-related kinase 6 (NEK6) expression in hepatic cell cancer
    Xiaolei Cao
    Department of Pathology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People s Republic of China
    Pathol Oncol Res 18:201-7. 2012
    b>Nek6 is a cell cycle regulatory gene, which can control cell proliferation and survival. Recent studies suggested that desregulation of Nek6 expression plays a key role in oncogenesis...
  5. ncbi Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo
    Jose M Lizcano
    Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:27839-49. 2002
    ..Recent work has implicated the NIMA (never in mitosis, gene A)-related kinase-6 (NEK6) as the enzyme that phosphorylates the hydrophobic motif of S6K1 in vivo...
  6. ncbi Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase
    Yoshihiro Hashimoto
    Department of Biochemistry, Nagoya City University Medical School, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya 467 8601, Japan
    Biochem Biophys Res Commun 293:753-8. 2002
    ..In the present study, we identified human Nek6 (hNek6), a member of the mammalian NIMA-related kinases...
  7. pmc Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression
    Joan Roig
    Department of Molecular Biology and the Diabetes Unit and Medical Services, Massachusetts General Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 16:1640-58. 2002
    ..Nercc1 binds to another NIMA-like kinase, Nek6, and also binds specifically to the Ran GTPase through both its catalytic and its RCC1-like domains, preferring ..
  8. ncbi Nek6 overexpression antagonizes p53-induced senescence in human cancer cells
    Hye Jin Jee
    Department of Biochemistry, College of Medicine, Dong A University, Busan, South Korea
    Cell Cycle 9:4703-10. 2010
    b>Nek6 is an NIMA-related kinase that plays a critical role in mitotic cell cycle progression. Recent studies have shown that Nek6 is upregulated in various human cancers, but the function of Nek6 in tumorigenesis is largely unknown...
  9. doi Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome
    Gabriela Vaz Meirelles
    Laboratório Nacional de Biociências, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil
    J Proteome Res 9:6298-316. 2010
    ..Human Nek6 was associated to carcinogenesis, but its interacting partners and signaling pathways remain elusive...
  10. ncbi Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins
    Eun Jeoung Lee
    School of Science Education, Chungbuk National University, Gaeshin dong, Heungdok gu, Chongju, Chungbuk 361 763, Republic of Korea
    Biochem Biophys Res Commun 358:783-8. 2007
    ..Here, we present evidence that human Nek6 is associated with Fe65...
  11. pmc Role of NEK6 in tumor promoter-induced transformation in JB6 C141 mouse skin epidermal cells
    Young Jin Jeon
    The Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA
    J Biol Chem 285:28126-33. 2010
    b>NEK6 (NIMA-related kinase 6) is a homologue of the Aspergillus nidulans protein NIMA (never in mitosis, gene A). We demonstrate that overexpression of NEK6 induces anchorage-independent transformation of JB6 Cl41 mouse epidermal cells...
  12. doi Nek6 mediates human cancer cell transformation and is a potential cancer therapeutic target
    Rounak Nassirpour
    Pfizer Global Research and Development, La Jolla Laboratories, 10724 Science Center Drive, San Diego, CA 92121, USA
    Mol Cancer Res 8:717-28. 2010
    We investigated the role of Nek6, a member of the NIMA-related serine/threonine kinase family, in tumorigenesis...
  13. pmc The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis
    Laura O'regan
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom
    Mol Cell Biol 29:3975-90. 2009
    b>Nek6 and Nek7 are members of the NIMA-related serine/threonine kinase family...
  14. ncbi Caught Nek-ing: cilia and centrioles
    Lynne M Quarmby
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
    J Cell Sci 118:5161-9. 2005
    ..b>Nek6, Nek7 and Nek9 also seem to regulate mitosis...
  15. ncbi Differential control of the NIMA-related kinases, Nek6 and Nek7, by serum stimulation
    Shigeru Minoguchi
    Division of Molecular and Cellular Immunology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, 812 8582, Fukuoka, Japan
    Biochem Biophys Res Commun 301:899-906. 2003
    ..Among these kinases, Nek6 and Nek7 are structurally related and constitute a subfamily in the NIMA/Nek family, although their functions still ..
  16. doi NEK6-mediated phosphorylation of human TPP1 regulates telomere length through telomerase recruitment
    Yugo Hirai
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto, 606 8501, Japan
    Genes Cells 21:874-89. 2016
    ..We show that TPP1 interacts with the cell cycle regulator kinase NEK6 in human cells...
  17. ncbi Proteomic analysis of brown planthopper: application to the study of carbamate toxicity
    Rashmi Sharma
    Institute of Insect and Animal Science, National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba, Ibaraki 305 8634, Japan
    Insect Biochem Mol Biol 34:425-32. 2004
    ..The differential expression of these proteins reflects the overall change in cellular structure and metabolism after insecticide treatment...
  18. pmc An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9
    Mark W Richards
    Institute of Cancer Research, London, UK
    Mol Cell 36:560-70. 2009
    Mitosis is controlled by multiple protein kinases, many of which are abnormally expressed in human cancers. Nek2, Nek6, Nek7, and Nek9 are NIMA-related kinases essential for proper mitotic progression...
  19. pmc hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells
    Ying Yan
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochem J 417:747-55. 2009
    ..The results of the present study show that, in mammalian cells, hVps34 activity is regulated through its interactions with hVps15, but is independent of Ca2+/CaM...
  20. pmc Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy
    Zhouyan Bian
    Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, P R China Cardiovascular Research Institute of Wuhan University, Wuhan, Hubei Province, P R China
    PLoS ONE 9:e96095. 2014
    ..NIMA (never in mitosis, gene A)-related kinase-6 (Nek6) is a cell cycle regulatory gene that could induce centriole duplication, and control cell proliferation and ..
  21. doi The potential role of the NEK6, AURKA, AURKB, and PAK1 genes in adenomatous colorectal polyps and colorectal adenocarcinoma
    Elmas Kasap
    Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey
    Tumour Biol 37:3071-80. 2016
    ..PAK1, NEK6, AURKA, AURKB, HDAC1, and HDAC7 were significantly more overexpressed in CRC subjects compared to the controls (p ..
  22. doi Structure, function, and evolution of plant NIMA-related kinases: implication for phosphorylation-dependent microtubule regulation
    Shogo Takatani
    Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka 3 1 1, Okayama, 700 8530, Japan
    J Plant Res 128:875-91. 2015
    ..Recent studies revealed that NEK6 of Arabidopsis thaliana regulates cell expansion and morphogenesis through β-tubulin phosphorylation and ..
  23. doi Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer
    Emre Gerçeker
    Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey
    Oncol Rep 34:1905-14. 2015
    ..In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified...
  24. pmc Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression
    Laura O'regan
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, England, UK
    J Cell Biol 209:349-58. 2015
    ..Hsp72 to the mitotic spindle is dependent on phosphorylation at Thr-66 within its nucleotide-binding domain by the Nek6 kinase. Phosphorylated Hsp72 concentrates on spindle poles and sites of MT-kinetochore attachment...
  25. pmc The cdr2(+) gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe
    C S Breeding
    Department of Cell Biology, Vanderbilt University, Nashville, Tennessee, 37212, USA
    Mol Biol Cell 9:3399-415. 1998
    ..Defects in cytokinesis are also generated by the overproduction of Cdr2p, but these defects are independent of wee1(+), suggesting that cdr2(+) encodes a second activity involved in cytokinesis...
  26. ncbi A second gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae
    T Nozaki
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Biochem Biophys Res Commun 228:452-8. 1996
    ..The results indicate that polyamine transport of yeast is regulated by multiple phosphorylation/dephosphorylation pathways...
  27. doi Nek9 phosphorylation of NEDD1/GCP-WD contributes to Plk1 control of γ-tubulin recruitment to the mitotic centrosome
    Sara Sdelci
    Cell Signaling Research Group, Molecular Medicine Program, Institute for Research in Biomedicine IRB Barcelona, Baldiri i Reixac, 10 12, 08028 Barcelona, Spain
    Curr Biol 22:1516-23. 2012
    ..requires its activation by Plk1-dependent phosphorylation but is independent from the downstream related kinases Nek6 and Nek7...
  28. pmc NIMA-related kinases regulate directional cell growth and organ development through microtubule function in Arabidopsis thaliana
    Hiroyasu Motose
    Division of Biological Sciences, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
    Plant Signal Behav 7:1552-5. 2012
    NIMA-related kinase 6 (NEK6) regulates cellular expansion and morphogenesis through microtubule organizaiton in Arabidopsis thaliana...
  29. doi The inhibition of Nek6 function sensitizes human cancer cells to premature senescence upon serum reduction or anticancer drug treatment
    Hye Jin Jee
    Department of Biochemistry, College of Medicine, Dong A University, Busan 602 714, South Korea
    Cancer Lett 335:175-82. 2013
    ..In this paper, we show that the inhibition of Nek6 expression by Nek6 siRNA-mediated knockdown or the overexpression of a dominant negative form of Nek6 (Nek6KM) ..
  30. pmc C. elegans NIMA-related kinases NEKL-2 and NEKL-3 are required for the completion of molting
    John Yochem
    Department of Molecular Biology, College of Agriculture and Natural Resources, University of Wyoming, Laramie, WY 82071, United States Department of Genetics, Cell Biology, and Development and the Developmental Biology Center, University of Minnesota, Minneapolis, MN 55455, United States
    Dev Biol 398:255-66. 2015
    ..NEKL-3 is highly similar to the mammalian NEK kinase family members NEK6 and NEK7...
  31. ncbi The ukb1 gene encodes a putative protein kinase required for bud site selection and pathogenicity in Ustilago maydis
    R B Abramovitch
    Biotechnology Laboratory, Department of Microbiology and Immunology, Faculty of Agricultural Sciences, University of British Columbia, 237 6174 University Blvd, Vancouver, BC, Canada
    Fungal Genet Biol 37:98-108. 2002
    ..In addition, the ukb1 gene influenced the ability of colonies to form aerial mycelia in response to environmental stimuli. Overall, the discovery of ukb1 reinforces the connection between morphogenesis and pathogenesis in U. maydis...
  32. doi Protein partners of KCTD proteins provide insights about their functional roles in cell differentiation and vertebrate development
    Mikhail Skoblov
    Research Center for Medical Genetics RAMS, Moscow, Russian Federation, Russia
    Bioessays 35:586-96. 2013
    ..predict that KCTD20 participates in the AKT-mTOR-p70 S6k signaling cascade, KCTD5 plays a role in cytokinesis in a NEK6 and ch-TOG-dependent manner, KCTD10 regulates the RhoA/RhoB pathway...
  33. pmc Structural analysis of the regulation of the DYNLL/LC8 binding to Nek9 by phosphorylation
    Pablo Gallego
    Structural Biology Unit, Institut de Biotecnologia i Biomedicina, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
    J Biol Chem 288:12283-94. 2013
    The NIMA family protein kinases Nek9/Nercc1, Nek6, and Nek7 constitute a signaling module activated in early mitosis involved in the control of spindle organization...
  34. ncbi PDK2: the missing piece in the receptor tyrosine kinase signaling pathway puzzle
    Lily Q Dong
    Dept of Cellular and Structural Biology, Univ of Texas Health Science Center, San Antonio, TX 78229, USA
    Am J Physiol Endocrinol Metab 289:E187-96. 2005
    ..integrin-linked kinase (ILK), p38 MAP kinase, protein kinase Calpha (PKCalpha), PKCbeta, the NIMA-related kinase-6 (NEK6), the mammalian target of rapamycin (mTOR), the double-stranded DNA-dependent protein kinase (DNK-PK), and the ..
  35. pmc Cell cycle regulation by the NEK family of protein kinases
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 125:4423-33. 2012
    ..In particular, NEK2, NEK6, NEK7 and NEK9 contribute to the establishment of the microtubule-based mitotic spindle, whereas NEK1, NEK10 and ..
  36. ncbi HcSTK, a Caenorhabditis elegans PAR-1 homologue from the parasitic nematode, Haemonchus contortus
    Sia Nikolaou
    Victorian Institute of Animal Science, Agriculture Victoria, 475 Mickleham Road, Attwood, Victoria 3049, Australia
    Int J Parasitol 32:749-58. 2002
    ..g. humans and rodents), invertebrates (e.g. insects and C. elegans) and yeast, suggests that HcSTK may be involved in a conserved signal transduction pathway...
  37. pmc Identification by high-throughput screening of viridin analogs as biochemical and cell-based inhibitors of the cell cycle-regulated nek2 kinase
    Daniel G Hayward
    Department of Biochemistry, University of Leicester, Leicester, UK
    J Biomol Screen 15:918-27. 2010
    ..The viridin-like compounds were >70-fold selective for Nek2 over Nek6 and Nek7 and inhibited the growth of human tumor cell lines at concentrations consistent with their biochemical ..
  38. doi RAF kinase inhibitory protein (RKIP) modulates cell cycle kinetics and motility
    Fahd Al-Mulla
    Department of Pathology, University of Kuwait, Faculty of Medicine, Health Sciences Center, 13110, Kuwait
    Mol Biosyst 7:928-41. 2011
    ..We show that RKIP depletion induces the expression of NEK6, a molecule known to enhance G2/M transition, and down-regulates G2/M checkpoint molecules like Aurora B, cyclin G1 ..
  39. doi Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy
    H Salem
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel
    Oncogene 29:4046-57. 2010
    ..Reduction in the activity of Nek7 or its close paralog, Nek6, has previously been shown to arrest cells in mitosis, mainly at metaphase...
  40. pmc The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation
    Joseph Rapley
    Department of Molecular Biology and Medical Services, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02114, USA
    J Cell Sci 121:3912-21. 2008
    b>Nek6 and Nercc1 (also known as Nek9) belong to the NIMA family of protein kinases. Nercc1 is activated in mitosis, whereupon it binds, phosphorylates and activates Nek6...
  41. ncbi Molecular cloning and characterization of a putative protein kinase gene from the thermophilic fungus Thermomyces lanuginosus
    Duo Chuan Li
    Department of Environmental Biology, Shandong Agricultural University, Taian, Shandong, People s Republic of China
    DNA Seq 18:423-33. 2007
    ..The putative protein kinase contained three arginine/serine-rich (SR) regions and two transmembrane domains. These showed that it might be a novel putative serine-threonine protein kinase...
  42. doi NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
    Bo Zhang
    State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant J 68:830-43. 2011
    ..In Arabidopsis, NEK6 is involved in the regulation of epidermal cell morphogenesis...
  43. ncbi Linking Nek6 to p53-induced senescence
    Min Jean Yin
    Pfizer Worldwide Research and Development San Diego, CA USA
    Cell Cycle 10:23-2. 2011
    ..Comment on: Jee HJ, et al. Cell Cycle 2010; 9:4703-10...
  44. doi NEK7 is essential for centriole duplication and centrosomal accumulation of pericentriolar material proteins in interphase cells
    Sunghwan Kim
    Department of Biological Sciences, Seoul National University, Seoul 151 747, Korea
    J Cell Sci 124:3760-70. 2011
    ..We also observed extra centriole formation in centrosome-directed NEK6-expressing cells, suggesting that NEK6 and NEK7 might share biological activities that induce centriole duplication...
  45. ncbi A role for Nek6 kinase activity in preventing senescence?
    Andrew M Fry
    Cell Cycle 10:19-20. 2011
  46. pmc Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain
    Gabriela V Meirelles
    Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil
    BMC Struct Biol 11:12. 2011
    ..Human Nek6, -7 and -9 are involved in the control of mitotic spindle formation, acting together in a mitotic kinase cascade, ..
  47. pmc DYNLL/LC8 protein controls signal transduction through the Nek9/Nek6 signaling module by regulating Nek6 binding to Nek9
    Laura Regué
    Cell Signaling Group, Molecular Medicine Program, Institute for Research in Biomedicine IRB Barcelona, 08028 Barcelona, Spain
    J Biol Chem 286:18118-29. 2011
    The NIMA family protein kinases Nek9/Nercc1 and the highly similar Nek6 and Nek7 form a signaling module activated in mitosis, when they are involved in the control of spindle organization and function...
  48. doi Nek6 suppresses the premature senescence of human cancer cells induced by camptothecin and doxorubicin treatment
    Hye Jin Jee
    Department of Biochemistry, College of Medicine, Dong A University, Busan, South Korea
    Biochem Biophys Res Commun 408:669-73. 2011
    Cellular senescence plays an important role in tumor suppression. The mitotic kinase Nek6 has recently been shown to be overexpressed in various cancers and has been implicated in tumorigenesis...
  49. doi NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana
    Hiroyasu Motose
    Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Plant J 67:993-1005. 2011
    NimA-related kinase 6 (NEK6) has been implicated in microtubule regulation to suppress the ectopic outgrowth of epidermal cells; however, its molecular functions remain to be elucidated...
  50. pmc The SINA E3 ligase OsDIS1 negatively regulates drought response in rice
    Yuese Ning
    Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha Hunan 410128, China
    Plant Physiol 157:242-55. 2011
    ..Together, these results demonstrate that OsDIS1 plays a negative role in drought stress tolerance through transcriptional regulation of diverse stress-related genes and possibly through posttranslational regulation of OsNek6 in rice...
  51. ncbi Structure and genomic sequence of the myotonic dystrophy (DM kinase) gene
    M S Mahadevan
    Department of Microbiology and Immunology, University of Ottawa, Ontario, Canada
    Hum Mol Genet 2:299-304. 1993
    ....
  52. pmc The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae
    S Bidlingmaier
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Cell Biol 21:2449-62. 2001
    ..Many Cbk1-related kinases have been shown to regulate cellular morphology...
  53. ncbi Isolation and characterization of two evolutionarily conserved murine kinases (Nek6 and nek7) related to the fungal mitotic regulator, NIMA
    M Kandli
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
    Genomics 68:187-96. 2000
    ..We describe here the isolation of two novel murine protein kinase genes, designated nek6 and nek7, which are highly similar to each other (87% amino acid identity in the predicted kinase domain)...
  54. ncbi Assignment of NEK6, a NIMA-related gene, to human chromosome 9q33. 3-->q34.11 by radiation hybrid mapping
    M Z Li
    Institute of Genetics, Fudan University, Shanghai, P R China
    Cytogenet Cell Genet 87:271-2. 1999
  55. ncbi Red1p, a MEK1-dependent phosphoprotein that physically interacts with Hop1p during meiosis in yeast
    T de los Santos
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, SUNY Stony Brook, Stony Brook, New York 11794 5215, USA
    J Biol Chem 274:1783-90. 1999
    ..Thus, MEK1-dependent phosphorylation of Red1p facilitates the formation of Hop1p/Red1p hetero-oligomers, thereby enabling the formation of functional AEs...
  56. ncbi The ltk gene family encodes novel receptor-like kinases with temporal expression in developing maize endosperm
    Z Li
    Department of Biological Sciences, Lehman College and The Graduate School and University Center, City University of New York, Bronx 10468, USA
    Plant Mol Biol 37:749-61. 1998
    ..These results suggest that members of the ltk gene family mediate signals associated with seed development and maturation...
  57. ncbi The control of morphogenesis in Saccharomyces cerevisiae by Elm1 kinase is responsive to RAS/cAMP pathway activity and tryptophan availability
    J M Garrett
    Department of Biology, Hamilton College, Clinton, NY 13323, USA
    Mol Microbiol 26:809-20. 1997
    ..A 'signal integration' model for the complex relationship of Elm1 and the RAS/cAMP pathway in controlling morphogenesis in response to nutrients is proposed...
  58. pmc Cloning and characterization of a putative human serine/threonine protein kinase transcriptionally modified during anisotonic and isotonic alterations of cell volume
    S Waldegger
    Physiologisches Institut I der Eberhard Karls Universität, D 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 94:4440-5. 1997
    ..The putative serine/threonine protein kinase h-sgk may provide a functional link between the cellular hydration state and metabolic control...
  59. ncbi A putative Plasmodium falciparum exported serine/threonine protein kinase
    J F Kun
    Australian Centre for International and Tropical Health and Nutrition, Royal Brisbane Hospital, Australia Qld
    Mol Biochem Parasitol 85:41-51. 1997
    ..This is the first putative protein kinase of P. falciparum to be exported from the parasite into its host cell...
  60. ncbi Cloning and characterization of a novel protein kinase that impairs osteoblast differentiation in vitro
    A E Kearns
    Division of Endocrinology, Diabetes, Metabolism and Nutrition, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 276:42213-8. 2001
    ..This novel kinase, therefore, is likely to play an important regulatory role in attenuating the program of osteoblast differentiation...
  61. ncbi CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases
    J J Kieber
    Department of Biology, University of Pennsylvania, Philadelphia 19104 6018
    Cell 72:427-41. 1993
    ..The CTR1 gene has been cloned, and the DNA sequences of four mutant alleles were determined. The gene encodes a putative serine/threonine protein kinase that is most closely related to the Raf protein kinase family...
  62. ncbi The YAL017 gene on the left arm of chromosome I of Saccharomyces cerevisiae encodes a putative serine/threonine protein kinase
    M W Clark
    Biology Department, McGill University, Montreal, Canada
    Yeast 9:543-9. 1993
    ..Gene disruption of YAL017 indicates that it is non-essential for growth on glucose...
  63. ncbi Cloning of a novel putative protein kinase having a leucine zipper domain from human brain
    U R Reddy
    Children s Hospital of Philadelphia, PA 19104
    Biochem Biophys Res Commun 202:613-20. 1994
    ..Steady-state mRNA levels for zpk are high in human brain and kidney. Further studies are required to evaluate the role of zpk in neuronal differentiation...
  64. ncbi Identification and characterization of DBK, a novel putative serine/threonine protein kinase from human endothelial cells
    W Chu
    Department of Inflammation Autoimmune Diseases, Hoffmann La Roche Inc, Roche Research Center, Nutley NJ 07110 1199
    Eur J Biochem 225:695-702. 1994
    ..Using somatic cell hybrids, the DBK gene has been localized to human chromosome 14. The ubiquitous expression and high degree of conservation of DBK across species suggest that DBK may play an important role in cell functions...
  65. ncbi The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster
    B Yun
    Waksman Institute, Rutgers University, Piscataway, New Jersey 08855 0759
    Genes Dev 8:1160-73. 1994
    ..These results demonstrate that the kinase encoded by Doa is required at multiple stages of development, for both differentiation and maintenance of specific cell types...
  66. ncbi Cloning of the gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae
    Y Kakinuma
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Biochem Biophys Res Commun 216:985-92. 1995
    ..The results suggest that spermine uptake by this organism is probably regulated by phosphorylation and dephosphorylation...
  67. ncbi A putative serine/threonine protein kinase encoded by the segment-polarity fused gene of Drosophila
    T Preat
    Centre de Génétique Moléculaire du CNRS, Universite P et M Curie, Gif sur Yvette, France
    Nature 347:87-9. 1990
    ..This result provides further insight into segment-polarity interactions and their role in pattern formation...
  68. ncbi Five novel elements involved in the regulation of mitosis in fission yeast
    E Warbrick
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Mol Gen Genet 232:440-6. 1992
    ....
  69. ncbi Isolation and characterization of a Paramecium cDNA clone encoding a putative serine/threonine protein kinase
    Satoru Wada
    Graduate School of Life Sciences, Tohoku University, Aoba Aramaki, Sendai 980 8578, Japan
    Genetica 131:307-14. 2007
    ..Comparisons of expression level have shown that pcstk1 is expressed equally in cells at different stages (sexual and asexual). We discussed the possibility, as in other organisms, that a family of MAP kinase genes exists in P. caudatum...
  70. pmc Nek6 is involved in G2/M phase cell cycle arrest through DNA damage-induced phosphorylation
    Min Young Lee
    Department of Biochemistry, College of Medicine, Dong A University, South Korea
    Cell Cycle 7:2705-9. 2008
    b>Nek6 is a recently identified NIMA-related kinase that is required for mitotic cell cycle progression. In the present study, we examined the role of Nek6 in the DNA damage response...
  71. doi Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content
    Jingyu Xu
    National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Canada
    Plant Biotechnol J 6:799-818. 2008
    ..Thus, alteration of this putative serine/threonine protein kinase site can be exploited to enhance DGAT1 activity, and expression of mutated DGAT1 can be used to enhance oil content...
  72. doi The future of RIP2/RICK/CARDIAK as a biomarker of the inflammatory response to infection
    Michelle L McCully
    Departments of Microbiology and Immunology and Medicine, The FOCIS Centre for Clinical Immunology and Immunotherapeutics, Robarts Research Institute, The University of Western Ontario, Ontario, Canada N6A 5K8
    Expert Rev Mol Diagn 8:257-61. 2008
    ....
  73. doi Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition
    Mukesh Jain
    Department of Plant Pathology, University of Florida, Gainesville, FL 32611 0680, USA
    Physiol Plant 134:161-73. 2008
    ..seed development. However, discordant in situ hybridization and immunolocalization data indicated that the expression of SbSnRK1b homologue in maize is under posttranscriptional control during endosperm development...
  74. doi A NIMA-related protein kinase suppresses ectopic outgrowth of epidermal cells through its kinase activity and the association with microtubules
    Hiroyasu Motose
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3 8 1 Komaba, Meguro ku, Tokyo 153 8902, Japan
    Plant J 54:829-44. 2008
    ..IBO1 is identical to NEK6, which encodes a Never In Mitosis A (NIMA)-related protein kinase (Nek) with sequence similarity to Neks involved ..
  75. ncbi Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal-cell morphogenesis in Arabidopsis
    Tatsuya Sakai
    RIKEN Plant Science Center, 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Plant J 53:157-71. 2008
    ..We further reveal that a NIMA-related protein kinase, NEK6, binds to the ARK family proteins and has pleiotropic effects on epidermal-cell morphogenesis, suggesting that NEK6 ..
  76. pmc Mitotic regulation by NIMA-related kinases
    Laura O'regan
    Department of Biochemistry, University of Leicester, Leicester, UK
    Cell Div 2:25. 2007
    ..Of these, there is now substantial evidence that Nek2, Nek6, Nek7 and Nek9 also regulate mitotic events...
  77. ncbi A putative serine/threonine protein kinase gene on chromosome III of Saccharomyces cerevisiae
    C Wilson
    Department of Cellular and Developmental Biology, University of Rome La Sapienza, Italy
    Yeast 8:71-7. 1992
    ..Reduced stringency hybridizations revealed the presence of another genomic sequence with high homology to the carboxy-terminal catalytic domain of KIN82...
  78. pmc Integrative approach for differentially overexpressed genes in gastric cancer by combining large-scale gene expression profiling and network analysis
    A Takeno
    Department of Surgery, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka Suita, Osaka 565 0871, Japan
    Br J Cancer 99:1307-15. 2008
    ..as the hub, and extracted seven candidates for gastric carcinogenesis (MMP7, SPARC, SOD2, INHBA, IGFBP7, NEK6, LUM). They were classified into two groups based on the correlation between expression level and stage...
  79. pmc Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)
    Gustavo Delhon
    Plum Island Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Greenport, New York 11944, USA
    J Virol 80:8439-49. 2006
    ....
  80. ncbi Identification and characterization of Crumbs homolog 2 gene at human chromosome 9q33.3
    Masuko Katoh
    M and M Medical BioInformatics, Narashino 275 0022, Japan
    Int J Oncol 24:743-9. 2004
    ..elegans crb. Comparative genomics revealed that CRB2-KIAA1608-LHX2-NEK6 locus at human chromosome 9q33.3 and CRB1-MGC27044-LHX9-NEK7 locus at human chromosome 1q31...
  81. ncbi The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis
    Min Jean Yin
    SUGEN, Inc, South San Francisco, California 94080 4811, USA
    J Biol Chem 278:52454-60. 2003
    ..Here, we demonstrate that human NIMA-related kinase 6 (Nek6) is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase...
  82. ncbi The related murine kinases, Nek6 and Nek7, display distinct patterns of expression
    Erez Feige
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Mech Dev 110:219-23. 2002
    b>Nek6 and Nek7 are evolutionarily conserved murine kinases structurally related to the Aspergillus mitotic-regulator NIMA (Genomics 68 (2000) 187)...
  83. ncbi Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3
    M Kimura
    Department of Molecular Pathobiochemistry, Gifu University School of Medicine, Gifu, Japan
    Cytogenet Cell Genet 94:33-8. 2001
    ..Its open reading frame encodes a 302-amino acid protein and is 77% identical to human NEK6 protein. Phylogenetic analysis suggests that NEK6, NEK7, and Caenorhabditis elegans F19H6...
  84. ncbi Protein kinases: getting NEKed for S6K activation
    D J Templeton
    Department of Pathology, University of Virginia Medical School, PO Box 800214, Charlottesville, VA 22908 0214, USA
    Curr Biol 11:R596-9. 2001
    The 70/85 kDa ribosomal protein S6 kinase is regulated through the concerted actions of multiple phosphatases and kinases. A newly identified S6 kinase kinase, NEK6, appears to provide the penultimate activation step.
  85. ncbi Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase
    C Belham
    Diabetes Unit, Medical Services, Massachusetts General Hospital, Boston, MA 02114, USA
    Curr Biol 11:1155-67. 2001
    ..g.,Thr412 in p70 S6 kinase (alpha 1). Phosphorylation of the former site is catalyzed by PDK1, whereas the kinase responsible for the phosphorylation of the latter site is not known...