NEIL2

Summary

Gene Symbol: NEIL2
Description: nei like DNA glycosylase 2
Alias: NEH2, NEI2, endonuclease 8-like 2, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, nei endonuclease VIII-like 2, nei homolog 2, nei-like protein 2
Species: human

Top Publications

  1. pmc Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activity
    Kishor K Bhakat
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    Nucleic Acids Res 32:3033-9. 2004
  2. pmc Functional variants of the NEIL1 and NEIL2 genes and risk and progression of squamous cell carcinoma of the oral cavity and oropharynx
    Xiadong Zhai
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 14:4345-52. 2008
  3. ncbi Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions
    Tapas K Hazra
    Department of Human Biological Chemistry and Genetics, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Rte 1079, Galveston, TX 77555, USA
    J Biol Chem 277:30417-20. 2002
  4. ncbi Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2
    Hong Dou
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 278:49679-84. 2003
  5. doi Single nucleotide polymorphisms 5' upstream the coding region of the NEIL2 gene influence gene transcription levels and alter levels of genetic damage
    Carla J Kinslow
    Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, TX 77555 1110, USA
    Genes Chromosomes Cancer 47:923-32. 2008
  6. pmc Increased risk of lung cancer associated with a functionally impaired polymorphic variant of the human DNA glycosylase NEIL2
    Sanjib Dey
    Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA
    DNA Repair (Amst) 11:570-8. 2012
  7. pmc Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex
    Donna D Zhang
    Department of Biochemistry, University of Missouri Columbia, M121 Medical Sciences Building, Columbia, MO 65212, USA
    Mol Cell Biol 24:10941-53. 2004
  8. pmc Structural analysis of the complex of Keap1 with a prothymosin alpha peptide
    Balasundaram Padmanabhan
    Genomic Sciences Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:233-8. 2008
  9. pmc Nucleotide, cytogenetic and expression impact of the human chromosome 8p23.1 inversion polymorphism
    Nina Bosch
    Genetic Causes of Disease Group, Genes and Disease Programme Center for Genomic Regulation CRG UPF, Barcelona, Catalonia, Spain
    PLoS ONE 4:e8269. 2009
  10. doi Discovery of the negative regulator of Nrf2, Keap1: a historical overview
    Ken Itoh
    Department of Stress Response Science, Hirosaki University Graduate School of Medicine, Japan
    Antioxid Redox Signal 13:1665-78. 2010

Research Grants

  1. Polymorphic variants of human DNA glycosylase NEIL2 and lung cancer susceptibilit
    Tapas K Hazra; Fiscal Year: 2010
  2. Repair of Oxidized Bases in Mammalian Genomes
    Sankar Mitra; Fiscal Year: 2012
  3. Regulation of Nrf2 Signaling
    Michael Freeman; Fiscal Year: 2009
  4. Repair of oxidative damage in mammalian genomes
    Tapas K Hazra; Fiscal Year: 2010
  5. MAMMALIAN OXIDATIVE DNA DAMAGE REPAIR
    Thomas Rosenquist; Fiscal Year: 2004
  6. HYPOXIA-INDUCED DNA DAMAGE AND REPAIR IN THE RAT BRAIN
    Ella W Englander; Fiscal Year: 2010

Scientific Experts

  • TAPAS HAZRA
  • Michael McMahon
  • M Takao
  • Minmin Liu
  • Takashi Kobunai
  • Ken Itoh
  • Yiguo Zhang
  • Peter Broderick
  • Ella Englander
  • Thomas A Rosenquist
  • Aimee L Eggler
  • Sampa Pal
  • Sankar Mitra
  • Istvan Boldogh
  • Masayuki Yamamoto
  • Kit I Tong
  • Susan S Wallace
  • Muralidhar L Hegde
  • Michael Freeman
  • Luisa Luna
  • Magnar Bjørås
  • Leopoldo Aguilera-Aguirre
  • Jia Zhou
  • Balasundaram Padmanabhan
  • Silje Z Krokeide
  • Sherif Z Abdel-Rahman
  • I R Grin
  • Aditi Das
  • Carla J Kinslow
  • Inga R Grin
  • Sanjiv Sur
  • Veslemøy Rolseth
  • Cynthia J Burrows
  • Aaron M Fleming
  • Attila Bacsi
  • Yasutake Katoh
  • Partha S Sarkar
  • Akira Kobayashi
  • Natalya A Smirnova
  • Pavana M Hegde
  • Kishor K Bhakat
  • Hong Dou
  • Christine Gran Neurauter
  • D O Zharkov
  • Amit K Maiti
  • Atsushi Katafuchi
  • Shigeyuki Yokoyama
  • Lucy Petrova
  • Alan E Tomkinson
  • Young Kwang Chae
  • Dmitry O Zharkov
  • Anirban Chakraborty
  • Trang Nhu Do
  • Ana Osorio
  • April M Averill
  • Nabiela Moolla
  • Xueqing Ba
  • Samuel H Wilson
  • Zsolt Radak
  • Victor Cardenas
  • Rajendra Prasad
  • Altaf H Sarker
  • Roman Corral
  • Rui Wang
  • Aishwarya Prakash
  • Elisabeth A Keitges
  • Navneet Ammal Kaidery
  • Bartosz Szczesny
  • Rajiv R Ratan
  • Dmitry M Hushpulian
  • Ana I Rojo
  • Santi M Mandal
  • Lee Wiederhold
  • Won Dong Kim
  • Rowena Hancock
  • Hua Wei
  • Mauro Longoni
  • Irina G Gazaryan
  • Sanjib Dey
  • Thomas G Wood
  • Keiko Taguchi
  • Veron Ramsuran
  • X Y Goh
  • Jon K Laerdahl
  • Huaxian Ma
  • Meryl S Lillenes
  • Shala Ghaderi Berntsson
  • Nina Bosch
  • Randa A El-Zein
  • Jeffrey K Wickliffe

Detail Information

Publications101 found, 100 shown here

  1. pmc Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activity
    Kishor K Bhakat
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    Nucleic Acids Res 32:3033-9. 2004
    ..coactivator p300 stably interacts with, and acetylates, the recently discovered human DNA glycosylase NEIL2, involved in the repair of oxidized bases both in vivo and in vitro...
  2. pmc Functional variants of the NEIL1 and NEIL2 genes and risk and progression of squamous cell carcinoma of the oral cavity and oropharynx
    Xiadong Zhai
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 14:4345-52. 2008
    Human DNA glycosylases NEIL1 and NEIL2 participate in oxidized base excision repair and protect cells from DNA damage...
  3. ncbi Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions
    Tapas K Hazra
    Department of Human Biological Chemistry and Genetics, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Rte 1079, Galveston, TX 77555, USA
    J Biol Chem 277:30417-20. 2002
    ..A. 99, 3523-3528). Here we report characterization of the second protein, originally named NEH2 and now renamed NEIL2 (Nei-like). The 37-kDa wild-type NEIL2 expressed in and purified from E...
  4. ncbi Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2
    Hong Dou
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 278:49679-84. 2003
    ..The newly discovered human DNA glycosylases, NEIL1 and NEIL2, are distinct in structural features and reaction mechanism from the previously characterized NTH1 and OGG1 but ..
  5. doi Single nucleotide polymorphisms 5' upstream the coding region of the NEIL2 gene influence gene transcription levels and alter levels of genetic damage
    Carla J Kinslow
    Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, TX 77555 1110, USA
    Genes Chromosomes Cancer 47:923-32. 2008
    b>NEIL2 (EC 4.2.99.18), a mammalian DNA glycosylase and ortholog of the bacterial Fpg/Nei, excises oxidized DNA lesions from bubble or single-stranded structures, suggesting its involvement in transcription-coupled DNA repair...
  6. pmc Increased risk of lung cancer associated with a functionally impaired polymorphic variant of the human DNA glycosylase NEIL2
    Sanjib Dey
    Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA
    DNA Repair (Amst) 11:570-8. 2012
    Human NEIL2, one of five oxidized base-specific DNA glycosylases, is unique in preferentially repairing oxidative damage in transcribed genes...
  7. pmc Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex
    Donna D Zhang
    Department of Biochemistry, University of Missouri Columbia, M121 Medical Sciences Building, Columbia, MO 65212, USA
    Mol Cell Biol 24:10941-53. 2004
    ..E3 ubiquitin ligase complex with Cul3 and Rbx1 that targets multiple lysine residues located in the N-terminal Neh2 domain of Nrf2 for ubiquitin conjugation both in vivo and in vitro...
  8. pmc Structural analysis of the complex of Keap1 with a prothymosin alpha peptide
    Balasundaram Padmanabhan
    Genomic Sciences Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:233-8. 2008
    ..which is formed by the double-glycine repeat and C-terminal region domains (Keap1-DC), interacts directly with the Neh2 domain of Nrf2...
  9. pmc Nucleotide, cytogenetic and expression impact of the human chromosome 8p23.1 inversion polymorphism
    Nina Bosch
    Genetic Causes of Disease Group, Genes and Disease Programme Center for Genomic Regulation CRG UPF, Barcelona, Catalonia, Spain
    PLoS ONE 4:e8269. 2009
    ..The identification of single nucleotide polymorphisms (SNPs) tightly linked to the genomic orientation of a given region should be useful to indirectly evaluate the genotypes of large genomic orientations in the individuals...
  10. doi Discovery of the negative regulator of Nrf2, Keap1: a historical overview
    Ken Itoh
    Department of Stress Response Science, Hirosaki University Graduate School of Medicine, Japan
    Antioxid Redox Signal 13:1665-78. 2010
    ..Keap1 suppresses Nrf2 activity by specifically binding to its evolutionarily conserved N-terminal Neh2 regulatory domain...
  11. ncbi Identification of the interactive interface and phylogenic conservation of the Nrf2-Keap1 system
    Makoto Kobayashi
    The Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
    Genes Cells 7:807-20. 2002
    ..To gain an insight into our present enigma as to how cells receive oxidative and electrophilic signals and transduce them to Nrf2, we have developed a zebrafish model system for molecular toxicological studies...
  12. ncbi AP endonuclease-independent DNA base excision repair in human cells
    Lee Wiederhold
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA
    Mol Cell 15:209-20. 2004
    ..The recently discovered mammalian DNA glycosylase/AP lyases, NEIL1 and NEIL2, unlike the previously characterized OGG1 and NTH1, generate DNA strand breaks with 3' phosphate termini...
  13. ncbi Rapid recognition of aberrant dHPLC elution profiles using the Transgenomic Navigator software
    James Colley
    Institute of Medical Genetics, Cardiff University, Health Park, Cardiff, CF14 4XN, United Kingdom
    Hum Mutat 26:165. 2005
    ..We used dHPLC to identify germline variants in MSH6, NEIL2, NEIL3, and OGG1 in 172 patients with multiple colorectal adenomas...
  14. pmc Oxidative DNA damage repair in mammalian cells: a new perspective
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of Biochemistry and Molecular Biology, 6 136 Medical Research Building, Route 1079, University of Texas Medical Branch, Galveston, TX 77555, USA
    DNA Repair (Amst) 6:470-80. 2007
    ....
  15. pmc Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress
    Soumita Das
    Sealy Center for Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 282:28474-84. 2007
    The recently characterized enzyme NEIL2 (Nei-like-2), one of the four oxidized base-specific DNA glycosylases (OGG1, NTH1, NEIL1, and NEIL2) in mammalian cells, has poor base excision activity from duplex DNA...
  16. pmc Deficiency of the oxidative damage-specific DNA glycosylase NEIL1 leads to reduced germinal center B cell expansion
    Hiromi Mori
    Laboratory for Immune Diversity, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    DNA Repair (Amst) 8:1328-32. 2009
    Mammalian cells possess multiple DNA glycosylases, including OGG1, NTH1, NEIL1, NEIL2 and NEIL3, for the repair of oxidative DNA damage...
  17. doi Analysis of DNA repair gene polymorphisms and survival in low-grade and anaplastic gliomas
    Shala Ghaderi Berntsson
    Department of Neuroscience, Neurology, University Hospital, 75185 Uppsala, Sweden
    J Neurooncol 105:531-8. 2011
    ..In this dataset, eight gene variants mapping to five different DNA repair genes (ATM, NEIL1, NEIL2, ERCC6 and RPA4) which were associated with survival...
  18. doi E-cadherin inhibits nuclear accumulation of Nrf2: implications for chemoresistance of cancer cells
    Won Dong Kim
    College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea
    J Cell Sci 125:1284-95. 2012
    ..between E-cadherin and Nrf2: E-cadherin bound the C-terminus of Nrf2, but not its N-terminus, which comprises the Neh2 domain responsible for phosphorylation of Ser40...
  19. pmc Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation
    Attila Bacsi
    Department of Microbiology and Immunology, School of Medicine, University of Texas Medical Branch, Galveston, TX 77555, USA
    DNA Repair (Amst) 12:18-26. 2013
    ..Decreased expression of the Nei-like glycosylases Neil1 and Neil2 that preferentially excise ring-opened purines and 5-hydroxyuracil, respectively, did not alter the above ..
  20. pmc Congenital diaphragmatic hernia interval on chromosome 8p23.1 characterized by genetics and protein interaction networks
    Mauro Longoni
    Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Am J Med Genet A 158:3148-58. 2012
    ..1 hotspot and products of previously known CDH causing genes, implicated GATA4, NEIL2, and SOX7 in diaphragmatic defects...
  21. pmc DNA glycosylases search for and remove oxidized DNA bases
    Susan S Wallace
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Burlington, Vermont
    Environ Mol Mutagen 54:691-704. 2013
    ..Also single-molecule approaches show that DNA glycosylases search for their substrates in a sea of undamaged DNA by using a wedge residue that is inserted into the DNA helix to probe for the presence of damage...
  22. doi The contribution of Nth and Nei DNA glycosylases to mutagenesis in Mycobacterium smegmatis
    Nabiela Moolla
    DST NRF Centre of Excellence for Biomedical TB Research, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand and the National Health Laboratory Service, P O Box 1038, Johannesburg 2000, South Africa
    DNA Repair (Amst) 13:32-41. 2014
    ..smegmatis (Msm), which retains a single nth homologue and duplications of the Fpg (fpg1 and fpg2) and Nei (nei1 and nei2) homologues...
  23. pmc DNA glycosylases involved in base excision repair may be associated with cancer risk in BRCA1 and BRCA2 mutation carriers
    Ana Osorio
    Human Genetics Group, Spanish National Cancer Centre CNIO, Madrid, Spain Biomedical Network on Rare Diseases CIBERER, Madrid, Spain
    PLoS Genet 10:e1004256. 2014
    ..The strongest evidence was for rs1466785 in the NEIL2 (endonuclease VIII-like 2) gene (HR: 1.09, 95% CI (1.03-1.16), p = 2...
  24. pmc Innate inflammation induced by the 8-oxoguanine DNA glycosylase-1-KRAS-NF-κB pathway
    Leopoldo Aguilera-Aguirre
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555
    J Immunol 193:4643-53. 2014
    ..e., NEIL1 and NEIL2) had no significant effect...
  25. pmc The NEIL glycosylases remove oxidized guanine lesions from telomeric and promoter quadruplex DNA structures
    Jia Zhou
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Nucleic Acids Res 43:4039-54. 2015
    ..However, NEIL1, NEIL2 and NEIL3 remove hydantoins from telomeric quadruplexes formed by five TTAGGG repeats much more rapidly than the ..
  26. pmc Neil2-null Mice Accumulate Oxidized DNA Bases in the Transcriptionally Active Sequences of the Genome and Are Susceptible to Innate Inflammation
    Anirban Chakraborty
    From the Department of Internal Medicine, Sealy Center for Molecular Medicine
    J Biol Chem 290:24636-48. 2015
    ..Here, we report the generation and characterization of mice lacking Neil2 (Nei-like 2)...
  27. doi Accelerating the Conformational Sampling of Intrinsically Disordered Proteins
    Trang Nhu Do
    Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada
    J Chem Theory Comput 10:5081-94. 2014
    ..The total simulation time was over 10 μs, and a 20-mer peptide derived from the Neh2 domain of the Nuclear factor erythroid 2-related factor 2 (Nrf2) protein was simulated...
  28. pmc Genomic landscape of DNA repair genes in cancer
    Young Kwang Chae
    Northwestern Medicine Developmental Therapeutics Institute, Chicago, IL, USA
    Oncotarget 7:23312-21. 2016
    ..and CNV frequencies offer insights into which genes may play tumor suppressive or oncogenic roles, such as NEIL2 and RRM2B, respectively...
  29. pmc Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells
    Lucy Petrova
    Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia, United States of America
    PLoS ONE 11:e0158581. 2016
    ..5-OHU induced sustained oncogenic signaling in Neil1-/-Neil2-/- mouse cells...
  30. ncbi Mapping of the GATA4, NEIL2, FDFT1 genes and CTSB-associated microsatellites to the centromeric region of BTA8
    A Plis-Finarov
    Institute of Animal Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
    Anim Genet 35:154-5. 2004
  31. ncbi Expression of the oxidative base excision repair enzymes is not induced in TK6 human lymphoblastoid cells after low doses of ionizing radiation
    M Inoue
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068, USA
    Radiat Res 161:409-17. 2004
    ..No induction at the transcriptional level of any of the base excision repair genes, NTH1 (NTHL1), OGG1, NEIL1, NEIL2, NEIL3, APE1, POLB, or accessory protein genes, LIG3, XRCC1 or XPG, was found at gamma-radiation doses ranging ..
  32. ncbi Molecular mechanism activating Nrf2-Keap1 pathway in regulation of adaptive response to electrophiles
    Ken Itoh
    JST ERATO Environmental Response Project, Center for TARA and Institute for Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Free Radic Biol Med 36:1208-13. 2004
    ..activity led to the identification of Keap1, which represses Nrf2 activity by directly binding to the N-terminal Neh2 domain...
  33. ncbi Evolutionary conserved N-terminal domain of Nrf2 is essential for the Keap1-mediated degradation of the protein by proteasome
    Yasutake Katoh
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Arch Biochem Biophys 433:342-50. 2005
    ..This process is dependent on Keap1, to which Nrf2 binds through the Neh2 domain...
  34. ncbi Purification and characterization of NEIL1 and NEIL2, members of a distinct family of mammalian DNA glycosylases for repair of oxidized bases
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of HBC and G, University of Texas Medical Branch, Galveston, USA
    Methods Enzymol 408:33-48. 2006
    NEIL1 and NEIL2 were newly discovered as mammalian orthologs of Escherichia coli Nei and Fpg, oxidized base-specific DNA glycosylases...
  35. pmc Role of human DNA glycosylase Nei-like 2 (NEIL2) and single strand break repair protein polynucleotide kinase 3'-phosphatase in maintenance of mitochondrial genome
    Santi M Mandal
    Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 287:2819-29. 2012
    ..Here we show the presence of the oxidized base-specific DNA glycosylase Nei-like 2 (NEIL2) and the DNA end-processing enzyme polynucleotide kinase 3'-phosphatase (PNKP) in purified human mitochondrial ..
  36. doi Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway
    Christine Gran Neurauter
    Department of Microbiology, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
    DNA Repair (Amst) 11:401-9. 2012
    ..BER is initiated by lesion-specific DNA glycosylases that recognize and remove the damaged base. NEIL1, NEIL2 and NEIL3 are three mammalian members of the Fpg/Nei DNA glycosylase family with similar enzymatic properties...
  37. pmc Association of polymorphisms in oxidative stress genes with clinical outcomes for bladder cancer treated with Bacillus Calmette-Guérin
    Hua Wei
    Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America
    PLoS ONE 7:e38533. 2012
    ..05). Six SNPs, including five in NEIL2 gene from the overall and BCG group remained significantly associated with recurrence after multiple comparison ..
  38. pmc Targeting Nrf2-mediated gene transcription by extremely potent synthetic triterpenoids attenuate dopaminergic neurotoxicity in the MPTP mouse model of Parkinson's disease
    Navneet Ammal Kaidery
    Department of Pharmacology and Toxicology, Georgia Health Sciences University, Augusta, GA 30912, USA
    Antioxid Redox Signal 18:139-57. 2013
    ..Nuclear factor E2-related factor 2 (Nrf2) is a redox-sensitive transcription factor that upregulates a battery of antioxidant response element (ARE)-driven antioxidative and cytoprotective genes that defend against oxidative stress...
  39. pmc Neil3, the final frontier for the DNA glycosylases that recognize oxidative damage
    Minmin Liu
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405 0086, USA
    Mutat Res 743:4-11. 2013
    ..Endonuclease VIII like proteins (Neil1, Neil2 and Neil3) are found in vertebrate genomes and are homologous to the well-characterized bacterial DNA glycosylases,..
  40. pmc Seaweed extracts and unsaturated fatty acid constituents from the green alga Ulva lactuca as activators of the cytoprotective Nrf2-ARE pathway
    Rui Wang
    Department of Medicinal Chemistry, University of Florida, Gainesville, FL 32610, USA
    Free Radic Biol Med 57:141-53. 2013
    ..Collectively, this study provides new insight into why consumption of dietary seaweed may have health benefits, and the identified compounds add to the list of chemopreventive dietary unsaturated fatty acids...
  41. doi Prenatal diagnosis of two fetuses with deletions of 8p23.1, critical region for congenital diaphragmatic hernia and heart defects
    Elisabeth A Keitges
    Department of Cytogenetics, Laboratory Corporation of America Dynacare, Seattle, WA 98122, USA
    Am J Med Genet A 161:1755-8. 2013
    ..Microarray analysis detected a 127-kb deletion which included the GATA4 and NEIL2 genes which was inherited from the mother...
  42. pmc Human NEIL3 is mainly a monofunctional DNA glycosylase removing spiroimindiohydantoin and guanidinohydantoin
    Silje Z Krokeide
    Department of Microbiology, University of Oslo, Oslo University Hospital, Rikshospitalet, PO Box 4950, Nydalen, NO 0424 Oslo, Norway
    DNA Repair (Amst) 12:1159-64. 2013
    ..Unlike NEIL1 and NEIL2, which possess a β,δ-elimination activity, NEIL3 mainly incised damaged DNA by β-elimination...
  43. pmc Neil3 and NEIL1 DNA glycosylases remove oxidative damages from quadruplex DNA and exhibit preferences for lesions in the telomeric sequence context
    Jia Zhou
    From the Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068 and
    J Biol Chem 288:27263-72. 2013
    ..We have tested the base excision activities of five mammalian DNA glycosylases (NEIL1, NEIL2, mNeil3, NTH1, and OGG1) on these lesion-containing quadruplex substrates and found that only mNeil3 had excision ..
  44. pmc Genetic variation in the base excision repair pathway, environmental risk factors, and colorectal adenoma risk
    Roman Corral
    Department of Preventive Medicine, Keck School of Medicine of USC, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 8:e71211. 2013
    ..Significant associations were also observed between SNPs in the NEIL2 gene and rectal adenoma risk. Three SNPS modified the effect of smoking (MUTYH interaction p = 0...
  45. pmc Structural investigation of a viral ortholog of human NEIL2/3 DNA glycosylases
    Aishwarya Prakash
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0068, United States
    DNA Repair (Amst) 12:1062-71. 2013
    ..Here we present the first crystal structure of a viral ortholog of the human NEIL2/NEIL3 proteins, Mimivirus Nei2 (MvNei2), determined at 2.04Å resolution...
  46. pmc Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain
    K Itoh
    Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
    Genes Dev 13:76-86. 1999
    ..The activation of Nrf2 leads in turn to the induction of phase II enzyme and antioxidative stress genes in response to electrophiles and reactive oxygen species...
  47. pmc Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants
    Albena T Dinkova-Kostova
    The Lewis B and Dorothy Cullman Cancer Chemoprotection Center, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:11908-13. 2002
    ..and purified murine Keap1 and demonstrated on native gels the formation of complexes of Keap1 with the Neh2 domain of Nrf2 and their concentration-dependent disruption by inducers such as sulforaphane and bis(2-..
  48. ncbi A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue
    Masashi Takao
    Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980 8575, Japan
    J Biol Chem 277:42205-13. 2002
    ..These results suggest that NEIL1 is a back-up glycosylase for NTH1 with unique substrate specificity and tissue-specific expression...
  49. ncbi A novel human DNA glycosylase that removes oxidative DNA damage and is homologous to Escherichia coli endonuclease VIII
    Viswanath Bandaru
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0068, USA
    DNA Repair (Amst) 1:517-29. 2002
    ..Furthermore, inactivation of active site residues shown to be important in Escherichia coli Nei inactivate the human enzyme. The hNEI1 gene is located on the long arm of chromosome 15 that is frequently deleted in human cancers...
  50. ncbi Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression
    Michael McMahon
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
    J Biol Chem 278:21592-600. 2003
    ..Within Nrf2, the N-terminal Neh2 domain is identified as the redox-sensitive degron...
  51. ncbi The novel DNA glycosylase, NEIL1, protects mammalian cells from radiation-mediated cell death
    Thomas A Rosenquist
    Department of Pharmacological Sciences, SUNY Stony Brook, Stony Brook, NY 11794, USA
    DNA Repair (Amst) 2:581-91. 2003
    ..Three human genes, designated NEIL1, NEIL2 and NEIL3, encode proteins that contain sequence homologies to Nei and Fpg...
  52. ncbi Phosphorylation of Nrf2 at Ser40 by protein kinase C in response to antioxidants leads to the release of Nrf2 from INrf2, but is not required for Nrf2 stabilization/accumulation in the nucleus and transcriptional activation of antioxidant response element
    David A Bloom
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 278:44675-82. 2003
    ..The PKC site at Ser40 is conserved among species and lies in the Neh2 domain, which interacts with INrf2...
  53. ncbi Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesions
    Atsushi Katafuchi
    Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi Hiroshima 739 8526, Japan
    J Biol Chem 279:14464-71. 2004
    ..However, detailed analysis of the cellular activity suggests that hNEIL1 has a significant role in the repair of 5S-Tg in human cells...
  54. ncbi Redox-regulated turnover of Nrf2 is determined by at least two separate protein domains, the redox-sensitive Neh2 degron and the redox-insensitive Neh6 degron
    Michael McMahon
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
    J Biol Chem 279:31556-67. 2004
    ..In homeostatic COS1 cells, the Neh2 degron confers on Nrf2 a half-life of less than 10 min...
  55. ncbi Identification of a zinc finger domain in the human NEIL2 (Nei-like-2) protein
    Aditi Das
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
    J Biol Chem 279:47132-8. 2004
    The recently identified human NEIL2 (Nei-like-2) protein, a DNA glycosylase/AP lyase specific for oxidatively damaged bases, shares structural features and reaction mechanism with the Escherichia coli DNA glycosylases, Nei and Fpg...
  56. ncbi Recognition of the oxidized lesions spiroiminodihydantoin and guanidinohydantoin in DNA by the mammalian base excision repair glycosylases NEIL1 and NEIL2
    M Katie Hailer
    Department of Chemistry, The University of Montana, Missoula, MT 59812, USA
    DNA Repair (Amst) 4:41-50. 2005
    ..Herein we report that two recently identified mammalian glycosylases, NEIL1 and NEIL2, showed a high affinity for recognition and cleavage of DNA containing Gh/Ia and Sp lesions...
  57. ncbi The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase beta bind microtubules in situ and in vitro
    Kimberly A Conlon
    Department of Pharmacological Sciences, School of Medicine University Hospital and Medical Center, State University of New York Stony Brook, NY 11794 8651, USA
    DNA Repair (Amst) 4:419-31. 2005
    ....
  58. pmc Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2
    Aimee L Eggler
    Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607, USA
    Proc Natl Acad Sci U S A 102:10070-5. 2005
    ..Under basal conditions, Keap1 sequesters Nrf2 in the cytoplasm by binding to its Neh2 domain...
  59. ncbi Differential modulation of base excision repair activities during brain ontogeny: implications for repair of transcribed DNA
    Ella W Englander
    Department of Surgery, The University of Texas Medical Branch, Shriners Hospitals for Children, 815 Market Street, Galveston, TX 77550, USA
    Mech Ageing Dev 127:64-9. 2006
    ..While a subset of BER enzymes, exhibited declining expression and excision activities, expression of NEIL1 and NEIL2 glycosylases increased during brain development...
  60. pmc Keap1 recruits Neh2 through binding to ETGE and DLG motifs: characterization of the two-site molecular recognition model
    Kit I Tong
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8572, Japan
    Mol Cell Biol 26:2887-900. 2006
    ..We found that the Neh2 domain of Nrf2 is an intrinsically disordered but biologically active regulatory domain containing a 33-residue ..
  61. ncbi Dimerization of substrate adaptors can facilitate cullin-mediated ubiquitylation of proteins by a "tethering" mechanism: a two-site interaction model for the Nrf2-Keap1 complex
    Michael McMahon
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
    J Biol Chem 281:24756-68. 2006
    ..facilitates ubiquitylation of the Nrf2 transcription factor but only when this substrate possesses within its Neh2 domain a second cryptic Keap1-binding site, the DLG motif, in addition to its previously described ETGE site...
  62. pmc Negative regulation of the Nrf1 transcription factor by its N-terminal domain is independent of Keap1: Nrf1, but not Nrf2, is targeted to the endoplasmic reticulum
    Yiguo Zhang
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK
    Biochem J 399:373-85. 2006
    ..Within acidic domain 1, residues 156-242 share 43% identity with the Neh2 (Nrf2-ECH homology 2) degron of Nrf2 that serves to destabilize this latter transcription factor through an ..
  63. ncbi Deoxyribophosphate lyase activity of mammalian endonuclease VIII-like proteins
    Inga R Grin
    SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia
    FEBS Lett 580:4916-22. 2006
    ..Here, we demonstrate that NEIL1 and NEIL2, mammalian homologs of bacterial endonuclease VIII, excise dRP by beta-elimination with the efficiency similar to ..
  64. pmc NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells
    Aditi Das
    Department of Biochemistry and Molecular Biology, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Route 1079, Galveston, TX 77555, USA
    DNA Repair (Amst) 5:1439-48. 2006
    ..barely active in removing 3' phosphate generated by the recently discovered mammalian DNA glycosylases NEIL1 and NEIL2. We showed earlier that the 3' phosphate generated by NEIL1 is efficiently removed by polynucleotide kinase (PNK) ..
  65. pmc Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predisposition
    Peter Broderick
    Section of Cancer Genetics, Brookes Lawley Building, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
    BMC Cancer 6:243. 2006
    ..It is conceivable that germline sequence variation in other BER pathway genes such as NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 also contribute to CRC susceptibility.
  66. ncbi Different organization of base excision repair of uracil in DNA in nuclei and mitochondria and selective upregulation of mitochondrial uracil-DNA glycosylase after oxidative stress
    M Akbari
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7006 Trondheim, Norway
    Neuroscience 145:1201-12. 2007
    ..The DNA glycosylases Nei-like DNA glycosylase 1 (NEIL1), Nei-like DNA glycosylase 2 (NEIL2), mitochondrial uracil-DNA glycosylase 1 (UNG1), nuclear uracil-DNA glycosylase 2 (UNG2) and endonuclease III-like ..
  67. ncbi Damage specificity of human DNA glycosylases for oxidative pyrimidine lesions
    Atsushi Katafuchi
    Graduate School of Science, Hiroshima University, Higashi Hiroshima 739 8526, Japan
    Nucleic Acids Symp Ser (Oxf) . 2004
    ..hNEIL2 exhibited a strong AP lyase activity but the N-glycosylase activity for the tested oxidative base lesions was marginal...
  68. ncbi Human polymorphic variants of the NEIL1 DNA glycosylase
    Laura M Roy
    Center for Research on Occupational and Environmental Toxicology, Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    J Biol Chem 282:15790-8. 2007
    ..reactive oxygen species is primarily initiated by a series of DNA glycosylases that include OGG1, NTH1, NEIL1, and NEIL2. To explore the functional significance of NEIL1, we recently reported that neil1 knock-out and heterozygotic mice ..
  69. ncbi Human non-synonymous single nucleotide polymorphisms can influence ubiquitin-mediated protein degradation
    Sevtap Savas
    Fred A Litwin Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    OMICS 11:200-8. 2007
    ..in the presence of nsSNPs in CCND3, PMS2, POLE4, SITPEC, and PPARG and putative PEST motifs were created in NEIL2, BIRC4, MLL2, and PPP1R15A...
  70. ncbi Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter
    Flor M Perez-Campo
    Stem Cell Biology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, UK
    Exp Cell Res 313:3604-15. 2007
    ..As proof-of-principle, we have utilized the knock-out targeting vector to modify the NEIL2 locus in ES cells...
  71. pmc Different electrostatic potentials define ETGE and DLG motifs as hinge and latch in oxidative stress response
    Kit I Tong
    Graduate School of Comprehensive Human Sciences, Center for TARA, JST ERATO Environmental Response Project, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 27:7511-21. 2007
    ..The DLG motif works to correctly position the lysines within the Nrf2 Neh2 domain for efficient ubiquitination...
  72. ncbi Subcellular localization and cytoplasmic complex status of endogenous Keap1
    Yoriko Watai
    Graduate School of Comprehensive Human Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Japan
    Genes Cells 12:1163-78. 2007
    ..an adaptor for Cullin-3-based ubiquitin ligase, and a regulator of Nrf2 activity through the interaction with Nrf2 Neh2 domain...
  73. ncbi Interaction of the human DNA glycosylase NEIL1 with proliferating cell nuclear antigen. The potential for replication-associated repair of oxidized bases in mammalian genomes
    Hong Dou
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
    J Biol Chem 283:3130-40. 2008
    NEIL1 and NEIL2 compose a family of DNA glycosylases that is distinct from that of the other two DNA glycosylases, OGG1 and NTH1, all of which are involved in repair of oxidized bases in mammalian genomes...
  74. doi Base excision repair activities in organotypic hippocampal slice cultures exposed to oxygen and glucose deprivation
    Veslemøy Rolseth
    Centre for Molecular Biology and Neuroscience, Institute of Medical Microbiology, University of Oslo, Rikshospitalet, N 0027 Oslo, Norway
    DNA Repair (Amst) 7:869-78. 2008
    ..Real-time RT-PCR showed that transcription of DNA glycosylases, including Ogg1, Nth1, Ung, Aag, Neil1 and Neil2 were not changed in response to OGD treatment (t=0 h)...
  75. pmc Structural insights into the similar modes of Nrf2 transcription factor recognition by the cytoplasmic repressor Keap1
    Balasundaram Padmanabhan
    Genomic Sciences Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    J Synchrotron Radiat 15:273-6. 2008
    ..The Neh2 domain of Nrf2 interacts with Keap1 at the bottom region of the Kelch/beta-propeller domain which is formed by ..
  76. ncbi Novel DNA glycosylases from Mycobacterium tuberculosis
    V S Sidorenko
    Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk 630090, Russia
    Biochemistry (Mosc) 73:442-50. 2008
    ..We have cloned and expressed two paralogs (Mtu-Nei2 and Mtu-Fpg2) from 36KAZ and KHA94 isolates of Mycobacterium tuberculosis and studied their ability to participate ..
  77. pmc Polynucleotide kinase as a potential target for enhancing cytotoxicity by ionizing radiation and topoisomerase I inhibitors
    N K Bernstein
    Department of Biochemistry, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada
    Anticancer Agents Med Chem 8:358-67. 2008
    ..agents including ionizing radiation, topoisomerase I inhibitors, and DNA repair glycosylases such as NEIL1 and NEIL2, often contain 5'-hydroxyl and/or 3'-phosphate termini...
  78. pmc Mutator phenotype of mammalian cells due to deficiency of NEIL1 DNA glycosylase, an oxidized base-specific repair enzyme
    Amit K Maiti
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
    DNA Repair (Amst) 7:1213-20. 2008
    The recently characterized NEIL1 and NEIL2 are distinct from the previously characterized mammalian DNA glycosylases (OGG1 and NTH1) involved in repair of oxidized bases because of the NEILs' preference for excising base lesions from ..
  79. pmc Widespread distribution of DNA glycosylases removing oxidative DNA lesions in human and rodent brains
    Veslemøy Rolseth
    Centre for Molecular Biology and Neuroscience, Institute of Medical Microbiology, University of Oslo, Rikshospitalet HF, Oslo, Norway
    DNA Repair (Amst) 7:1578-88. 2008
    ..In mammalian cells at least five different DNA glycosylases with overlapping substrate specificity, NEIL1, NEIL2, NEIL3, OGG1 and NTH1, remove oxidative DNA base lesions...
  80. pmc Prospective type 1 and type 2 disulfides of Keap1 protein
    Ryan Holland
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA
    Chem Res Toxicol 21:2051-60. 2008
    ..The energy minimizations indicated marked alterations in structure with a substantial constriction of Neh2 binding domain of the Keap1 Kelch repeat domain...
  81. doi A simple method for amino acid selective isotope labeling of recombinant proteins in E. coli
    Kit I Tong
    Center for Tsukuba Advanced Research Alliance, Graduate School of Comprehensive Human Sciences, JST ERATO Environmental Response Project, University of Tsukuba, Tsukuba, 305 8577, Japan
    J Biomol NMR 42:59-67. 2008
    ..The sequential backbone resonance assignment of the Neh2 domain of Nrf2 transcriptional factor, an intrinsically disordered protein with high spectral degeneracy, was ..
  82. doi Expression and purification of NEIL3, a human DNA glycosylase homolog
    Silje Z Krokeide
    Centre for Molecular Biology and Neuroscience, Department of Molecular Biology, Institute of Microbiology, Rikshospitalet Medical Centre, Sognsvannsveien 28, N0027 Oslo, Norway
    Protein Expr Purif 65:160-4. 2009
    ..Thus, a mammalian family of three proteins (NEIL1, NEIL2 and NEIL3) that showed homology to the Escherichia coli Fpg/Nei DNA glycosylases was identified...
  83. doi Human Nei-like protein NEIL3 has AP lyase activity specific for single-stranded DNA and confers oxidative stress resistance in Escherichia coli mutant
    Masashi Takao
    Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Aoba ku, Sendai, Japan
    Genes Cells 14:261-70. 2009
    ..damage to DNA, cells contain several DNA glycosylases including OGG1, NTH1 and the Nei-like proteins, NEIL1 and NEIL2. A third Nei-like protein, NEIL3, is composed of an amino-terminal Nei-like domain and an unknown carboxy-terminal ..
  84. pmc Cul3-mediated Nrf2 ubiquitination and antioxidant response element (ARE) activation are dependent on the partial molar volume at position 151 of Keap1
    Aimee L Eggler
    Center for Pharmaceutical Biotechnology and the Department of Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago, IL 60607, USA
    Biochem J 422:171-80. 2009
    ..This model has significant implications for how electrophiles which modify Cys(151), disrupt the repressive function of Keap1...
  85. ncbi Heavy metal ions affect the activity of DNA glycosylases of the fpg family
    I R Grin
    Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, pr Lavrentieva 8, Novosibirsk, Russia
    Biochemistry (Mosc) 74:1253-9. 2009
    ..enzymes formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease VIII (Nei) and their eukaryotic homologs NEIL1, NEIL2, and NEIL3 define the Fpg family of DNA glycosylases, which initiate the process of repair of oxidized DNA bases...
  86. pmc Regulatory regions responsive to oxidative stress in the promoter of the human DNA glycosylase gene NEIL2
    Carla J Kinslow
    Department of Obstetrics and Gynecology, The University of Texas Medical Branch, Galveston, TX 77555 1062, USA
    Mutagenesis 25:171-7. 2010
    ..If not repaired, these lesions could lead to genomic instability and, potentially, to cancer development. NEIL2 (EC 4.2.99...
  87. pmc Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1
    Sampa Pal
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110 USA
    J Gastroenterol Hepatol 25:627-34. 2010
    ..In the current study we examined the induction of reactive oxygen species (ROS) resulting from HCV infection and evaluated its effect on the host DNA damage and repair machinery...
  88. pmc Inactivation of NEIL2 DNA glycosylase by pyridoxal phosphate reveals a loop important for substrate binding
    Inga R Grin
    SB RAS Institute of Chemical Biology and Fundamental Medicine, 8 Lavrentieva Ave, Novosibirsk 630090, Russia
    Biochem Biophys Res Commun 394:100-5. 2010
    ..Of six enzymes tested, only endonuclease VIII-like protein 2 (NEIL2) was significantly inhibited by PLP. The inhibition was due to Schiff base formation between PLP and the enzyme...
  89. pmc The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo
    Minmin Liu
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0086, USA
    Proc Natl Acad Sci U S A 107:4925-30. 2010
    ..have been identified in mammals that are homologous to the Escherichia coli Fpg and Nei proteins, Neil1, Neil2, and Neil3...
  90. pmc Specific Inhibition of NEIL-initiated repair of oxidized base damage in human genome by copper and iron: potential etiological linkage to neurodegenerative diseases
    Muralidhar L Hegde
    Department of Biochemistry, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 285:28812-25. 2010
    ..repair pathway, initiated with one of four major DNA glycosylases: NTH1 or OGG1 (of the Nth family) or NEIL1 or NEIL2 (of the Nei family)...
  91. pmc Induction of NEIL1 and NEIL2 DNA glycosylases in aniline-induced splenic toxicity
    Huaxian Ma
    Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Toxicol Appl Pharmacol 251:1-7. 2011
    ..of NEIL1/2 was assayed using a bubble structure substrate containing 5-OHU (preferred substrates for NEIL1 and NEIL2) and by quantitating the cleavage products. Aniline treatment led to a 1...
  92. doi Molecular mechanisms of the Keap1–Nrf2 pathway in stress response and cancer evolution
    Keiko Taguchi
    Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, 2 1 Seiryo cho, Aoba ku, Sendai 980 8575, Japan
    Genes Cells 16:123-40. 2011
    ..in which one molecule of Nrf2 associates with two molecules of Keap1 by using two binding sites within the Neh2 domain of Nrf2. This two-site binding appears critical for Nrf2 ubiquitination...
  93. doi Integrative analysis of array-comparative genomic hybridisation and matched gene expression profiling data reveals novel genes with prognostic significance in oesophageal adenocarcinoma
    X Y Goh
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge, UK
    Gut 60:1317-26. 2011
    ..This study aimed to use genome-wide genomic and expression data to enhance the understanding of OAC pathogenesis and identify groups with differential outcomes...
  94. pmc Duffy-null-associated low neutrophil counts influence HIV-1 susceptibility in high-risk South African black women
    Veron Ramsuran
    HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R Mandela School of Medicine, University of KwaZulu Natal, Durban, South Africa
    Clin Infect Dis 52:1248-56. 2011
    ..The influence of pre-seroconversion levels of peripheral blood cell counts (PBCs) on the risk of acquiring human immunodeficiency virus (HIV)-1 infection among Africans is unknown...
  95. ncbi Eukaryotic endonuclease VIII-like proteins: new components of the base excision DNA repair system
    I R Grin
    Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia
    Biochemistry (Mosc) 76:80-93. 2011
    ..In 2002, three new human DNA glycosylases (NEIL1, NEIL2, and NEIL3) were discovered, all homologous to endonuclease VIII, a bacterial protein, which also participates in ..
  96. pmc Development of Neh2-luciferase reporter and its application for high throughput screening and real-time monitoring of Nrf2 activators
    Natalya A Smirnova
    Burke Medical Research Institute, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, White Plains, NY 10605, USA
    Chem Biol 18:752-65. 2011
    ..To directly monitor stabilization of Nrf2, we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-..
  97. doi DNA base excision repair gene polymorphisms modulate human cognitive performance and decline during normal life span
    Meryl S Lillenes
    Centre for Molecular Biology and Neuroscience, Institute of Microbiology, University of Oslo, Norway
    Mech Ageing Dev 132:449-58. 2011
    ..MutYH(His324Gln), APE1(Gln51His), APE1(Glu148Asp), XRCC1(Lys298Asn), XRCC1(Arg7Leu), NEIL1(Asp252Asn), and NEIL2(Arg257Leu). XRCC1(Arg7Leu) and PolB(Pro242Arg) were characterized by single nucleotide variations (≤0...
  98. doi Peptide inhibitors of the Keap1-Nrf2 protein-protein interaction
    Rowena Hancock
    Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, London WC1N 1AX, UK
    Free Radic Biol Med 52:444-51. 2012
    ..We describe the structure-activity relationships for a series of peptides based upon regions of the Nrf2 Neh2 domain, of varying length and sequence, that interact with the Keap1 Kelch domain and disrupt the interaction with ..
  99. doi Signaling pathways activated by the phytochemical nordihydroguaiaretic acid contribute to a Keap1-independent regulation of Nrf2 stability: Role of glycogen synthase kinase-3
    Ana I Rojo
    Departamento de Bioquímica e Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Centro de Investigación en Red sobre Enfermedades Neurodegenerativas, Instituto de Investigación Sanitaria La Paz, Madrid 28029, Spain
    Free Radic Biol Med 52:473-87. 2012
    ..The stability of Nrf2 involves the interaction of two degradation domains, designated Neh2 and Neh6, with the E3 ubiquitin ligase adaptors, Keap1 and β-TrCP, respectively...
  100. ncbi REG4, NEIL2, and BIRC5 gene expression correlates with gamma-radiation sensitivity in patients with rectal cancer receiving radiotherapy
    Takashi Kobunai
    Department of Surgery, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi ku, Tokyo 173 8605, Japan
    Anticancer Res 31:4147-53. 2011
    ..The present study aimed to identify genes that influence the susceptibility of cancer cells to radiation...
  101. doi Inherited predisposition to colorectal adenomas caused by multiple rare alleles of MUTYH but not OGG1, NUDT1, NTH1 or NEIL 1, 2 or 3
    A R Dallosso
    Institute of Medical Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Gut 57:1252-5. 2008
    ..MUTYH functions in the DNA base excision repair pathway and has a key role in the repair of oxidative DNA damage...

Research Grants6

  1. Polymorphic variants of human DNA glycosylase NEIL2 and lung cancer susceptibilit
    Tapas K Hazra; Fiscal Year: 2010
    ..We have found that downregulation of NEIL2, a recently identified oxidized base-specific DNA glycosylase, increases mutation frequency in the hprt gene of ..
  2. Repair of Oxidized Bases in Mammalian Genomes
    Sankar Mitra; Fiscal Year: 2012
    ..In our previous project period we characterized human glycosylases (NEIL1 and NEIL2) with overlapping substrate preferences as OGG1 and NTH1...
  3. Regulation of Nrf2 Signaling
    Michael Freeman; Fiscal Year: 2009
    ..Aim 2 will determine if key Keap1 Cys residues regulate association between the Neh2 domain of Nrf2 and Keap1 following reaction with thiol-reactive electrophiles...
  4. Repair of oxidative damage in mammalian genomes
    Tapas K Hazra; Fiscal Year: 2010
    ..b>NEIL2 has a 4- to 5-fold higher affinity and activity for 5-OHU in bubble vs duplex DNA, and it excises 8-oxoG from ..
  5. MAMMALIAN OXIDATIVE DNA DAMAGE REPAIR
    Thomas Rosenquist; Fiscal Year: 2004
    ..The effects of altering the level of ogg1 gene expression on the rate of mutagenesis and tumorigenesis also will be determined. ..
  6. HYPOXIA-INDUCED DNA DAMAGE AND REPAIR IN THE RAT BRAIN
    Ella W Englander; Fiscal Year: 2010
    ..abstract_text> ..