MUT

Summary

Gene Symbol: MUT
Description: methylmalonyl-CoA mutase
Alias: MCM, methylmalonyl-CoA mutase, mitochondrial, methylmalonyl Coenzyme A mutase, methylmalonyl-CoA isomerase, mutant methylmalonyl CoA mutase, truncated methylmalonyl CoA mutase
Species: human

Top Publications

  1. doi Minichromosome maintenance protein expression in benign nevi, dysplastic nevi, melanoma, and cutaneous melanoma metastases
    Alan S Boyd
    Department of Medicine Dermatology, Vanderbilt University, Nashville, Tennessee, USA
    J Am Acad Dermatol 58:750-4. 2008
  2. ncbi Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
  3. pmc Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
    D Sean Froese
    Structural Genomics Consortium, University of Oxford OX3 7DU, United Kingdom
    J Biol Chem 285:38204-13. 2010
  4. doi Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
    Tóshiko Takahashi-Íñiguez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
    Biochem Biophys Res Commun 404:443-7. 2011
  5. ncbi Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
  6. pmc Loss of allostery and coenzyme B12 delivery by a pathogenic mutation in adenosyltransferase
    Michael Lofgren
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0600, USA
    Biochemistry 50:5790-8. 2011
  7. pmc Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes
    L Folgueira
    Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 70:223-31. 1996
  8. pmc Liver-directed recombinant adeno-associated viral gene delivery rescues a lethal mouse model of methylmalonic acidemia and provides long-term phenotypic correction
    Nuria Carrillo-Carrasco
    Organic Acid Research Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892, USA
    Hum Gene Ther 21:1147-54. 2010
  9. ncbi Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
    E L LeCluyse
    Department of Pharmacology, Toxicology, and Therapeutics, Center for Environmental and Occupational Health, Kansas University Medical Center, Kansas City, Kansas, USA
    Toxicol In Vitro 14:101-15. 2000
  10. ncbi Molecular analysis and heterologous expression of the gene encoding methylmalonyl-coenzyme A mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
    W Zhang
    Department of Microbiology, Shanghai Institute of Plant Physiology, Academia Sinica, P R China
    Appl Biochem Biotechnol 82:209-25. 1999

Research Grants

  1. Anja Katrin Bielinsky; Fiscal Year: 2016
  2. James A Borowiec; Fiscal Year: 2014
  3. Functional analysis of Chk2 in DNA damage response
    Jun Hyun Kim; Fiscal Year: 2013
  4. Genetic Analysis of MSM Protein Function in S. Pombe
    Susan L Forsburg; Fiscal Year: 2012
  5. Yilun Liu; Fiscal Year: 2015
  6. GENETIC AND MOLECULAR ANALYSIS OF YEAST DNA REPLICATION
    Robert A Sclafani; Fiscal Year: 2012
  7. Kate E Coleman; Fiscal Year: 2015
  8. Regulation of MCM helicase loading and activation in the model system Saccharomyc
    Rebecca Ferguson; Fiscal Year: 2013
  9. The Role of the MCM2-7 Complex in the Replication Fork Processivity
    Anthony Schwacha; Fiscal Year: 2013
  10. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010

Detail Information

Publications516 found, 100 shown here

  1. doi Minichromosome maintenance protein expression in benign nevi, dysplastic nevi, melanoma, and cutaneous melanoma metastases
    Alan S Boyd
    Department of Medicine Dermatology, Vanderbilt University, Nashville, Tennessee, USA
    J Am Acad Dermatol 58:750-4. 2008
    Minichromosome maintenance (MCM) proteins are a recently elucidated group of polypeptides intimately involved in DNA replication and appreciable only in cycling cells...
  2. ncbi Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
    ..These studies provide insights into the energetics of interaction between the radical enzyme methylmalonyl-CoA mutase and MeaB, which are discussed...
  3. pmc Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
    D Sean Froese
    Structural Genomics Consortium, University of Oxford OX3 7DU, United Kingdom
    J Biol Chem 285:38204-13. 2010
    ..Cbl) is essential to the function of two human enzymes, methionine synthase (MS) and methylmalonyl-CoA mutase (MUT)...
  4. doi Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
    Tóshiko Takahashi-Íñiguez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
    Biochem Biophys Res Commun 404:443-7. 2011
    ..examined the influence of human MMAA protein on the kinetics of the reaction catalyzed by methylmalonyl-CoA mutase (MCM) by testing both purified recombinant proteins in vitro...
  5. ncbi Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
    ....
  6. pmc Loss of allostery and coenzyme B12 delivery by a pathogenic mutation in adenosyltransferase
    Michael Lofgren
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0600, USA
    Biochemistry 50:5790-8. 2011
    ..and an escort that delivers the product, adenosylcobalamin (AdoCbl or coenzyme B(12)), to methylmalonyl-CoA mutase (MCM), resulting in holoenzyme formation. Failure to assemble holo-MCM leads to methylmalonic aciduria...
  7. pmc Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes
    L Folgueira
    Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 70:223-31. 1996
    ..In addition, cotransfection of a negative dominant molecule of PKC-zeta (PKC-zeta mut) with NF-kappa B-dependent reporter genes selectively inhibits the HIV- but not phorbol myristate acetate- or ..
  8. pmc Liver-directed recombinant adeno-associated viral gene delivery rescues a lethal mouse model of methylmalonic acidemia and provides long-term phenotypic correction
    Nuria Carrillo-Carrasco
    Organic Acid Research Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892, USA
    Hum Gene Ther 21:1147-54. 2010
    ..disorder caused by a deficiency of the ubiquitously expressed mitochondrial enzyme, methylmalonyl-CoA mutase (MUT)...
  9. ncbi Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
    E L LeCluyse
    Department of Pharmacology, Toxicology, and Therapeutics, Center for Environmental and Occupational Health, Kansas University Medical Center, Kansas City, Kansas, USA
    Toxicol In Vitro 14:101-15. 2000
    ..Hepatocytes cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) or modified Chee's medium (MCM) maintained similar levels of total GST protein regardless of the matrix configuration or corresponding cell ..
  10. ncbi Molecular analysis and heterologous expression of the gene encoding methylmalonyl-coenzyme A mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
    W Zhang
    Department of Microbiology, Shanghai Institute of Plant Physiology, Academia Sinica, P R China
    Appl Biochem Biotechnol 82:209-25. 1999
    ..A (CoA) into methylmalonyl-CoA, catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM), represents an important source of building blocks for rifamycin SV biosynthesis...
  11. doi Cobalamin deficiency results in an abnormal increase in L-methylmalonyl-co-enzyme-A mutase expression in rat liver and COS-7 cells
    Motoyuki Nakao
    Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka 599 8531, Japan
    Br J Nutr 101:492-8. 2009
    ..study was to examine the effects of cobalamin (Cbl) on the activity and expression of L-methylmalonyl-CoA mutase (MCM) in rat liver and cultured COS-7 cells...
  12. pmc Primary structure and activity of mouse methylmalonyl-CoA mutase
    M F Wilkemeyer
    Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030
    Biochem J 271:449-55. 1990
    ..Genetic deficiency of this enzyme in man causes an often fatal disorder of organic acid metabolism termed mut methylmalonicacidaemia...
  13. ncbi Effect of fetuin, a TGFbeta antagonist and pentoxifylline, a cytokine antagonist on hepatic stellate cell function and fibrotic parameters in fibrosis
    Monica Verma-Gandhu
    Department of Pharmacology Faculty of Medicine Dalhousie University, Canada
    Eur J Pharmacol 572:220-7. 2007
    We have previously shown that monocyte conditioned medium (MCM) from patients with liver fibrosis stimulated proliferation of hepatic stellate cells (HSCs), the major cell involved in hepatic fibrosis...
  14. ncbi Initiating DNA synthesis: from recruiting to activating the MCM complex
    M Lei
    Dept of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cell Sci 114:1447-54. 2001
    ..A key component of the pre-RC is the hexameric MCM complex, which is also the presumed helicase of the growing forks...
  15. pmc Genomic structure of murine methylmalonyl-CoA mutase: evidence for genetic and epigenetic mechanisms determining enzyme activity
    M F Wilkemeyer
    Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030
    Biochem J 296:663-70. 1993
    Methylmalonyl-CoA mutase (MCM) is a nuclear-encoded mitochondrial matrix enzyme...
  16. ncbi Mutation and haplotype analyses of the MUT gene in Japanese patients with methylmalonic acidemia
    Osamu Sakamoto
    Department of Pediatrics, Tohoku University School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai, 980 8574, Japan
    J Hum Genet 52:48-55. 2007
    Methylmalonic acidemia (MMA) is caused by a deficiency in the activity of L: -methylmalonyl-CoA mutase (MCM), a vitamin B12 (or cobalamin, Cbl)-dependent enzyme...
  17. pmc Adenoviral-mediated correction of methylmalonyl-CoA mutase deficiency in murine fibroblasts and human hepatocytes
    Randy J Chandler
    National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Med Genet 8:24. 2007
    ..by deficiency of the mitochondrial localized, 5'deoxyadenosylcobalamin dependent enzyme, methylmalonyl-CoA mutase (MUT)...
  18. ncbi Cloning, expression and sequence analysis of the genes encoding the heterodimeric methylmalonyl-CoA mutase of Porphyromonas gingivalis W50
    C A Jackson
    Biochemistry and Molecular Biology Unit, School of Dental Science, University of Melbourne, Australia
    Gene 167:127-32. 1995
    Two genes that encode methylmalonyl-CoA mutase (MCM) have been characterised in Porphyromonas gingivalis W50 (Pg)...
  19. pmc Adenovirus-mediated gene delivery rescues a neonatal lethal murine model of mut(0) methylmalonic acidemia
    Randy J Chandler
    Genetic Diseases Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 19:53-60. 2008
    ..MMA), an autosomal recessive metabolic disorder, is most often caused by mutations in methylmalonyl-CoA mutase (MUT)...
  20. doi Theoretical analysis of the diradical nature of adenosylcobalamin cofactor-tyrosine complex in B12-dependent mutases: inspiring PCET-driven enzymatic catalysis
    Pawel M Kozlowski
    Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, USA
    J Phys Chem B 114:5928-39. 2010
    ..In particular, methylmalonyl-CoA mutase (MCM) and glutamate mutase (GLM) enzymes have been studied; in the case of MCM, the significance of the Y89 residue has ..
  21. ncbi Experimental study on mechanism and rarity of metastases in skeletal muscle
    Chenghua Luo
    Department of General Surgery, 301 Hospital, Beijing 100853, China
    Chin Med J (Engl) 115:1645-9. 2002
    ..To investigate the reasons for the rarity of metastases in skeletal muscle...
  22. pmc Association of germline mutations in the fumarate hydratase gene and uterine fibroids in women with hereditary leiomyomatosis and renal cell cancer
    Laveta Stewart
    Division of Cancer Epidemiology and Genetics, Genetic Epidemiology Branch, National Cancer Institute, National Institutes of Health, Rockville, MD 20892 7231, USA
    Arch Dermatol 144:1584-92. 2008
    ..To investigate the risk of uterine fibroids and other reproductive risk factors in women with hereditary leiomyomatosis and renal cell cancer (HLRCC)...
  23. doi Comparing the General Practice Research Database and the UK Epilepsy and Pregnancy Register as tools for postmarketing teratogen surveillance: anticonvulsants and the risk of major congenital malformations
    Rachel A Charlton
    Department of Pharmacy and Pharmacology, University of Bath, Bath, UK
    Drug Saf 34:157-71. 2011
    ....
  24. ncbi [Couples' knowledge and attitudes regarding the practice of family planning in the jungle of Peru]
    Doris de la Cruz-Peñarán
    Instituto Nacional de Salud Pública INSP, Avenida Universidad 655, colonia Santa María, Ahuacatitlán 62508 Cuernavaca, Morelos, Mexico
    Salud Publica Mex 45:461-71. 2003
    ..To assess the association of knowledge and attitudes about family planning (FP) with its practice in couples of the Peruvian jungle region...
  25. pmc Analysis of genomic instability in Li-Fraumeni fibroblasts with germline p53 mutations
    P K Liu
    Division of Laboratory Medicine, Department of Tumor Biology, The University of Texas MD Anderson Cancer Center, Houston 77030, USA
    Oncogene 12:2267-78. 1996
    ..We used p53(wt/mut) and p53(mut/-) LFS fibroblasts, and p53(-/-) non-LFS cells...
  26. pmc Endothelial cell-derived lipase mediates uptake and binding of high-density lipoprotein (HDL) particles and the selective uptake of HDL-associated cholesterol esters independent of its enzymic activity
    Juliane G Strauss
    Institute of Molecular Biology, Biochemistry and Microbiology, University of Graz, Harrachgasse 21, A 8010 Graz, Austria
    Biochem J 368:69-79. 2002
    ..by the finding that, in contrast with EDL, the amount of cell-surface-bound enzymically inactive mutant EDL (MUT-EDL) was not reduced in the presence of HDL and foetal calf serum...
  27. doi Flavin adenine dinucleotide status and the effects of high-dose riboflavin treatment in short-chain acyl-CoA dehydrogenase deficiency
    Bianca T van Maldegem
    Department of Pediatrics, University of Amsterdam, Amsterdam, The Netherlands
    Pediatr Res 67:304-8. 2010
    ..in a prospective open-label cohort study involving 16 patients with SCADD, subdivided into mutation/mutation (mut/mut), mutation/variant (mut/var), and variant/variant (var/var) genotype groups...
  28. ncbi T-->G or T-->A mutation introduced in the branchpoint consensus sequence of intron 4 of lecithin:cholesterol acyltransferase (LCAT) gene: intron retention causing LCAT deficiency
    M Li
    Atherosclerosis Specialty Laboratory, Department of Pathology and Laboratory Medicine, St Paul s Hospital and University of British Columbia, 1081 Burrard Street, Vancouver, British Columbia, Canada
    Biochim Biophys Acta 1391:256-64. 1998
    ..e., LCAT Int-4 MUT-1 (T-->G) and LCAT Int-4 MUT-2 (T-->A)...
  29. ncbi Long-lasting effects of chronic ozone exposure on rat nasal epithelium
    J R Harkema
    Department of Pathology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA
    Am J Respir Cell Mol Biol 20:517-29. 1999
    ..We have recently reported that long-term inhalation exposure to ozone causes mucous-cell metaplasia (MCM) in the surface epithelium lining the nasal airways of F344 rats...
  30. ncbi Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris
    Ramon Ramon
    Departament d Enginyeria Quimica, ETSE, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    J Biotechnol 130:39-46. 2007
    ..Remarkably, a different substrate consumption behaviour was observed depending on the host's phenotype (Mut(+) or Mut(s)) in batch cultures: when the methanol assimilation capacity is genetically reduced (Mut(s) phenotype), ..
  31. pmc Involvement of toll-like receptor 4 in alveolar bone loss and glucose homeostasis in experimental periodontitis
    K Watanabe
    Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612 7212, USA
    J Periodontal Res 46:21-30. 2011
    ..We therefore hypothesized that mice with periodontitis and a TLR4 loss-of-function (LOF) mutation fed a high-fat (HF) diet would develop improved glucose homeostasis compared with wild-type (WT) animals with periodontitis fed a HF diet...
  32. ncbi The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts
    M A Madine
    Wellcome CRC Institute, Cambridge, UK
    Curr Biol 5:1270-9. 1995
    A complex of MCM proteins is implicated in ensuring that DNA replicates only once in each cell cycle, by 'replication licensing'...
  33. ncbi Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus
    Floriana Carpentieri
    Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via P Castellino 111, Napoli 80131, Italy
    J Biol Chem 277:12118-27. 2002
    Mini-chromosome Maintenance (MCM) proteins play an essential role in both initiation and elongation phases of DNA replication in Eukarya...
  34. pmc Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection
    Saira Sarfraz
    1Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
    BMC Microbiol 8:133. 2008
    ..and normal histology (n = 5) were analyzed by immunohistochemistry using antibodies against proliferation marker Mcm-2, G1 phase marker Cyclin D1, S phase marker Cyclin A, cell cycle regulators p21 (CDK inhibitor) and p53 (tumor ..
  35. ncbi [Histologic study of local infiltration of spinal bone giant cell tumor and chordoma]
    Jie Lan
    Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China
    Zhonghua Wai Ke Za Zhi 46:1808-11. 2008
    ..To study the local infiltration length of spinal bone giant cell tumor and chordoma in various para-tumorous tissues...
  36. pmc Virulence variation among isolates of western equine encephalitis virus in an outbred mouse model
    Christopher H Logue
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA
    J Gen Virol 90:1848-58. 2009
    ..f.u. of each virus. Two WEEV strains, McMillan (McM) and Imperial 181 (IMP), were the most divergent of the six in genome sequence; McM caused 100 % mortality by 5 ..
  37. pmc Cloning and characterization of mouse intestinal MUC3 mucin: 3' sequence contains epidermal-growth-factor-like domains
    L L Shekels
    Department of Medicine, University of Minnesota and VA Medical Center, Minneapolis, MN 55417, USA
    Biochem J 330:1301-8. 1998
    ..We report here the cloning of a mouse caecal mucin (MCM). Amino acid analysis of purified MCM revealed a high content of serine (10.8%) and threonine (25.1%)...
  38. ncbi Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II
    Reiko Matsui
    Vascular Biology Unit, Whitaker Cardiovascular Institute and Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118 2393, USA
    Circulation 112:257-63. 2005
    ..We determined the hypertensive and vascular hypertrophic response to Ang II in G6PD-deficient mice...
  39. ncbi Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein
    Biagio Pucci
    Istituto di Biochimica delle Proteine, Consiglio Nazionale Ricerche, Via P Castellino, 111, 80131 Napoli, Italy
    J Biol Chem 282:12574-82. 2007
    Mini-chromosome maintenance (MCM) proteins form ring-like hexameric complexes that are commonly believed to act as the replicative DNA helicase at the eukaryotic/archaeal DNA replication fork...
  40. ncbi Improvement of dissolution rate of piroxicam by inclusion into MCM-41 mesoporous silicate
    V Ambrogi
    Dipartimento di Chimica e Tecnologia del Farmaco, Via del Liceo 1, Perugia, Italy
    Eur J Pharm Sci 32:216-22. 2007
    The aim of the present paper was the use of mesoporous silicate MCM-41 to increase the dissolution rate of piroxicam, a non-steroidal anti-inflammatory drug-class II of the Biopharmaceutic Classification System...
  41. doi Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
    Alison D Walters
    Department of Biology Area 5, PO Box 373, University of York, York YO10 5YW, UK
    Biochem Soc Trans 37:1-6. 2009
    ..Key proteins involved in the initiation and elongation phases of DNA replication include the MCM (minchromosome maintenance) proteins, MCM2-MCM7, a family of six related proteins believed to act as the ..
  42. doi Accelerated oxidation of epinephrine by silica nanoparticles
    Zhimin Tao
    Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA
    Langmuir 25:10183-8. 2009
    We have measured the influence of mesoporous silica (MCM-41 and SBA-15) nanoparticles and dense silica nanoparticles on epinephrine oxidation, a pH-dependent reaction, whose rate is small in acidic or neutral solutions but much greater ..
  43. ncbi Study on the indoor volatile organic compound treatment and performance assessment with TiO2/MCM-41 and TiO2/quartz photoreactor under ultraviolet irradiation
    Chia Wei Tsai
    Department of Environmental Engineering, National I Lan University, Taiwan, Republic of China
    J Air Waste Manag Assoc 58:1266-73. 2008
    ..activated by visible irradiation and packed with TiO2/quartz or TiO2/mobile catalytic material number 41 (MCM-41)...
  44. ncbi Immunohistochemical evaluation of metallothionein, Mcm-2 and Ki-67 antigen expression in tumors of the adrenal cortex
    Artur Szajerka
    Second Department of General and Oncological Surgery, Medical University, M Curie Sklodowska Street 66, Poland
    Anticancer Res 28:2959-65. 2008
    The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these ..
  45. ncbi The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep
    G Cringoli
    Dipartimento di Patologia e Sanita Animale, Facolta di Medicina Veterinaria, Universita degli Studi di Napoli Federico II, CREMOPAR Regione Campania, Via Della Veterinaria 1, 80137 Napoli, Italy
    Vet Parasitol 123:121-31. 2004
    ..For each McMaster slide, the GI strongyle and D. dendriticum egg counts were performed under one grid (McM 0.15 ml), two grids (McM 0.3 ml), one chamber (McM 0.5 ml), and both chambers (McM 1.0 ml)...
  46. doi Induction of glioma apoptosis by microglia-secreted molecules: The role of nitric oxide and cathepsin B
    So Young Hwang
    Department of Physiology and Biophysics, and Center for Advanced Medical Education by BK21 Project, Inha University, College of Medicine, Nam Ku, Incheon 402 751, Korea
    Biochim Biophys Acta 1793:1656-68. 2009
    ..Microglia conditioned culture medium (MCM) actively stimulated apoptotic death of glioma cells, and the effects of MCM prepared from LPS- or IFN-gamma-..
  47. doi Determination of very low levels of gold and palladium in wastewater and soil samples by atomic absorption after preconcentration on modified MCM-48 and MCM-41 silica
    H Ebrahimzadeh
    Department of Chemistry, Shahid Beheshti University, G C, Evin, Tehran, Iran
    Talanta 81:1183-8. 2010
    ..Extraction was investigated on adsorbents prepared by grafting aminopropyl on the surface of MCM-41 and MCM-48 mesoporous silica...
  48. ncbi Omeprazole treatment of Korean patients: effects on gastric pH and gastrin release in relation to CYP2C19 geno- and phenotypes
    Hyung Keun Roh
    Department of Laboratory Medicine, Division of Clinical Pharmacology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Basic Clin Pharmacol Toxicol 95:112-9. 2004
    ..patients with acid related disease were genotyped for CYP2C19 by allele specific PCR (wt/wt, CYP2C19*1/*1; wt/mut, CYP2C19*1/*2 or *1/*3; mut/mut, CYP2C19*2/*2, *2/*3 or *3/*3)...
  49. ncbi Photophysical Properties of Lanthanide Hybrids Covalently Bonded To Functionalized MCM-41 by Modified Aromatic Carboxylic Acids
    Ying Li
    Department of Chemistry, Tongji University, Shanghai, 200092, People s Republic of China
    J Fluoresc 19:191-201. 2009
    b>MCM-41 mesoporous silica has been functionalized with aromatic carboxylic acids salicylic acid (Sal) and 2-hydroxyl-3-methylbenzoic acid (HMBA) through co-condensation approach of tetraethoxysilane (TEOS) in the presence of the ..
  50. pmc A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae
    Bo Huang
    Department of Molecular Biology, Umea University, 901 87 Umea, Sweden
    RNA 14:2183-94. 2008
    ..tRNA(mcm5s2UUU Lys), and tRNA(mcm5s2UUG Gln) 3' of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U)...
  51. doi Novel and existing mutations in the tyrosine kinase domain of the epidermal growth factor receptor are predictors of optimal resectability in malignant peritoneal mesothelioma
    Jason M Foster
    Division of Surgical Oncology, Creighton University Medical Center, Creighton University, Omaha, NE 68131, USA
    Ann Surg Oncol 16:152-8. 2009
    ..and their tumors were probed for point mutations in the catalytic TK domain of epidermal growth factor receptor (mut+)...
  52. pmc Specific targeting to B cells by lipid-based nanoparticles conjugated with a novel CD22-ScFv
    Kristin Loomis
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Exp Mol Pathol 88:238-49. 2010
    ..Therefore, we generated a mutant of the targeting segment of HA22 (a CD22 scFv) to increase its soluble expression (mut-HA22), and conjugated it to the surface of sonicated liposomes to generate immunoliposomes (mut-HA22-liposomes)...
  53. pmc The Nrf2-inducible antioxidant defense in astrocytes can be both up- and down-regulated by activated microglia:Involvement of p38 MAPK
    Fernando Correa
    Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, Sweden
    Glia 59:785-99. 2011
    The effects of microglia-conditioned medium (MCM) on the inducible Nrf2 system in astrocyte-rich cultures were investigated by determination of glutathione (GSH) levels, γglutamylcysteine ligase (γGCL) activity, the protein levels of ..
  54. pmc Immunohistochemical estimation of cell cycle phase in laryngeal neoplasia
    P Chatrath
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 2XZ, UK
    Br J Cancer 95:314-21. 2006
    ..Antibodies against the markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67, and putative markers of cell cycle phase, cyclin D1 (G1-phase), cyclin A (S-phase), cyclin B1 (G2-..
  55. doi Effects of lipophilic components on the compatibility of lipid-based formulations with hard gelatin capsules
    Feng Jing Chen
    Boehringer Ingelheim Pharmaceuticals Inc, 900 Ridgebury Road, Ridgefield, Connecticut 06877, USA
    J Pharm Sci 99:128-41. 2010
    ..1) and an additional lipophilic excipient (Capmul MCM) significantly affected the activity of PG in the fills and the equilibrium of PG between capsule shells and fills...
  56. ncbi Nuclear envelope alterations in fibroblasts from LGMD1B patients carrying nonsense Y259X heterozygous or homozygous mutation in lamin A/C gene
    Antoine Muchir
    INSERM U582, Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, 75013, Paris, France
    Exp Cell Res 291:352-62. 2003
    ..limb-girdle muscular dystrophy and from her deceased newborn grandchild carrying, respectively, a heterozygous (+/mut) and a homozygous (mut/mut) nonsense Y259X mutation...
  57. ncbi Iliac cancellous bone in drug addicts: a histomorphometric study
    K H Schiwy-Bochat
    Institute of Forensic Medicine, Aachen University of Technology, Germany
    J Forensic Sci 44:249-52. 1999
    ..37 +/- 5.77% (mean, SD), controls 22.23 +/- 5.08%, p = 0.434; Tb.Th: 172.67 +/- 36.83 mcm, controls 169.73 +/- 36.13 mcm, p = 0.764)...
  58. ncbi A mutant streptokinase lacking the C-terminal 42 amino acids is less reactive with preexisting antibodies in patient sera
    I Torrens
    Division of Pharmaceutical, Centro de Ingenieria Genetica y Biotecnologia, Havana, Cuba
    Biochem Biophys Res Commun 266:230-6. 1999
    ..An SKC-2 deletion mutant (mut-C42), lacking the same 42 C-terminal residues, was constructed and expressed in Escherichia coli...
  59. ncbi Promoter methylation status of the DNA repair genes hMLH1 and MGMT in gastric carcinoma and metaplastic mucosa
    N Oue
    First Department of Pathology, Hiroshima University School of Medicine, Hiroshima, Japan
    Pathobiology 69:143-9. 2001
    ..DNA repair genes human Mut L homologue 1 (hMLH1) and O(6)-methylguanine-DNA methyltransferase (MGMT) have been shown to be hypermethylated in ..
  60. ncbi DNA synthesis and Bcl-2 expression during development of mucous cell metaplasia in airway epithelium of rats exposed to LPS
    Yohannes Tesfaigzi
    Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr, SE, Albuquerque, NM 87108, USA
    Am J Physiol Lung Cell Mol Physiol 286:L268-74. 2004
    ..environmental toxins and allergens may cause proliferation of airway epithelial cells and mucous cell metaplasia (MCM); however, it is unclear to what extent proliferating cells differentiate into mucus-storing cells and contribute ..
  61. ncbi Characterization of micro-mesoporous materials from nitrogen and toluene adsorption: experiment and modeling
    Peter I Ravikovitch
    Center for Modeling and Characterization of Nanoporous Materials, TRI Princeton, 601 Prospect Ave, Princeton, New Jersey 08540, USA
    Langmuir 22:513-6. 2006
    Universal mechanisms of adsorption and capillary condensation of toluene and nitrogen on ordered MCM-41 and PHTS materials are studied by means of high-resolution experiments and Monte Carlo molecular simulations...
  62. ncbi Catalytic activity of MCM-48-, SBA-15-, MCF-, and MSU-type mesoporous silicas modified with Fe3+ species in the oxidative dehydrogenation of ethylbenzene in the presence of N2O
    Piotr Kuśtrowski
    Jagiellonian University, Faculty of Chemistry, Ingardena 3, 30 060 Krakow, Poland
    J Phys Chem A 109:9808-15. 2005
    b>MCM-48, SBA-15, MCF, and MSU mesoporous silicas were used as supports for a deposition of Fe oxide species. Iron was introduced using two different methods: the wetness impregnation and the molecular designed dispersion (MDD)...
  63. ncbi Vibrational study on the local structure of post-synthesis and hybrid mesoporous materials: are there fundamental distinctions?
    Pedro D Vaz
    Department of Chemistry and Biochemistry, CQB, Faculty of Science, University of Lisbon, C8 Campo Grande, 1749 016 Lisboa, Portugal
    Chemistry 13:7874-82. 2007
    Organic-inorganic mesoporous materials of the MCM-41 type are important materials that can be prepared by either post-synthesis or one-pot synthesis procedures...
  64. ncbi Exceptional performance of sulfonic acid-incorporated-MCM-41 mesoporous materials prepared using a silane containing polysulfide linkages in the acetylation of anisole
    O Zoon Kwon
    School of Applied Chemical Engineering, Chonnam National University, YongBong 300, Gwangju 500 757, Republic of Korea
    Chem Commun (Camb) . 2007
    Sulfonic acid-incorporated-MCM-41 mesoporous materials prepared using a silane containing tetrasulfide linkages showed exceptional yields of the acetylated product in the acetylation of anisole due to their high content of strong acid ..
  65. pmc Isolation of a Drosophila amplification origin developmentally activated by transcription
    Fang Xie
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:9651-6. 2008
    ..By sequential chromatin immunoprecipitation, we show that the MCM complex and RNAPII are bound to the same 100-500 bp pieces of chromatin during late origin firing...
  66. ncbi Sensitizers on inorganic carriers for decomposition of the chemical warfare agent yperite
    Bogdan Cojocaru
    University of Bucharest, Department of Chemical Technology and Catalysis, B dul Regina Elisabeta 4 12, Bucharest 030016, Romania
    Environ Sci Technol 42:4908-13. 2008
    ..as photoactive components encapsulated inside the cavities of zeolite Y or the mesoporous channels of MCM-41 or supported on silica or titania-silica was tested for the photocatalytic decomposition of yperite...
  67. pmc Mcm4 C-terminal domain of MCM helicase prevents excessive formation of single-stranded DNA at stalled replication forks
    Naoki Nitani
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka 560 0043, Japan
    Proc Natl Acad Sci U S A 105:12973-8. 2008
    The minichromosome maintenance (MCM) helicase, composed of subunits Mcm2-7, is essential for the initiation and elongation phases of DNA replication...
  68. ncbi Catalytic ozonation of toluene using zeolite and MCM-41 materials
    C W Kwong
    Environmental Engineering Program, School of Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
    Environ Sci Technol 42:8504-9. 2008
    The effects of passing ozone over different zeolite and MCM-41 materials to remove toluene were investigated. Different ozone-to-toluene ratios were used to evaluate the catalytic performance during ozonation...
  69. pmc Dystrophia myotonia: why focus on foci?
    R P Junghans
    Departments of Surgery and Medicine, Boston University School of Medicine, Roger Williams Medical Center, Providence, RI 02908, USA
    Eur J Hum Genet 17:543-53. 2009
    ..Transcription of this gene yields a long CUGn-containing mutant (mut) RNA, in which clinical disease is associated with repeats of n=100-5000...
  70. doi Functional screen of human MCM2-7 variant alleles for disease-causing potential
    Nathan A Steere
    Department of Genetics, Cell Biology and Development, College of Biological Sciences, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, United States
    Mutat Res 666:74-8. 2009
    ..Previously, we reported the recovery of the first viable Mcm mutant allele (named Mcm4(Chaos3)) in mice...
  71. pmc MDM2 inhibitor MI-319 in combination with cisplatin is an effective treatment for pancreatic cancer independent of p53 function
    Asfar S Azmi
    Department of Pathology, Karmanos Cancer Institute, Wayne State University, School of Medicine, Detroit, MI, United States
    Eur J Cancer 46:1122-31. 2010
    ..In principle, these SMIs are not effective against tumours with mutation in the tumour suppressor p53 (mut-p53), which is known to be present in approximately 50% of all cancers...
  72. doi A single subunit MCM6 from pea promotes salinity stress tolerance without affecting yield
    Hung Quang Dang
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
    Plant Mol Biol 76:19-34. 2011
    ..The MCMs provide DNA unwinding function during the DNA replication. Since MCM proteins play essential role in cell division and most likely are affected during stress conditions therefore their ..
  73. doi Gas-phase photooxidation of alkenes by V-doped TiO2-MCM-41: mechanistic insights of ethylene photooxidation and understanding the structure-activity correlation
    Kaustava Bhattacharyya
    Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India
    Chemistry 17:12310-25. 2011
    Nanoparticles of Ti(0.95)V(0.05)O(2) were found to be impregnated in the hexagonal channels of the MCM-41 host, with a distribution of some particles on the surface, thus leading to an effective variation in the particle size as a ..
  74. doi Plant MCM proteins: role in DNA replication and beyond
    Narendra Tuteja
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
    Plant Mol Biol 77:537-45. 2011
    Mini-chromosome maintenance (MCM) proteins form heterohexameric complex (MCM2-7) to serve as licensing factor for DNA replication to make sure that genomic DNA is replicated completely and accurately once during S phase in a single cell ..
  75. doi Mitsuaria chitosanase with unrevealed important amino acid residues: characterization and enhanced production in Pichia pastoris
    Nan Peng
    State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People s Republic of China
    Appl Microbiol Biotechnol 97:171-9. 2013
    ..One of the P. pastoris transformants with Mut(S) phenotype was found to produce 1,480.2 ± 340...
  76. doi Causes of death of mutation carriers in Finnish Lynch syndrome families
    Kirsi Pylvänäinen
    Department of Surgery, Jyvaskyla Central Hospital, Jyvaskyla, Finland
    Fam Cancer 11:467-71. 2012
    ..The cumulative overall and cancer specific death rates were significantly increased in Mut+ compared to Mut- family members...
  77. ncbi Catalytic activity of (Fe2O3)-MCM-41-nPrNH2 magnetically recoverable nanocatalyst for the synthesis of phenylpyrido[4,3-d]pyrimidins
    Shahnaz Rostamizadeh
    Department of Chemistry, K N Toosi University of Technology, PO Box 15875 4416, Tehran, Iran
    J Nanosci Nanotechnol 13:4925-33. 2013
    ..from the reaction of (E)-3,5-bis(benzylidene)-4-piperidones and guanidine carbonate in the presence of (Fe2O3)-MCM-41-nPrNH2 as a magnetically recoverable nanocatalyst under solvent free conditions...
  78. doi Regulation of chromosome dynamics by Hsk1/Cdc7 kinase
    Seiji Matsumoto
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8613, Japan
    Biochem Soc Trans 41:1712-9. 2013
    ..Cdc7 (cell division cycle 7) kinase family, and promotes initiation of chromosome replication by phosphorylating Mcm (minichromosome maintenance) subunits, essential components for the replicative helicase...
  79. doi Nose to brain microemulsion-based drug delivery system of rivastigmine: formulation and ex-vivo characterization
    Brijesh M Shah
    a Department of Pharmaceutics, B V Patel PERD Centre, Ahmedabad, Gujarat, India
    Drug Deliv 22:918-30. 2015
    ..Rivastigmine-loaded ME system containing 8% w/w Capmul MCM EP, 44% w/w Labrasol:Transcutol-P (1:1) and 48% w/w distilled water was formulated, whereas 0...
  80. doi Wet-STEM tomography: principles, potentialities and limitations
    Karine Masenelli-Varlot
    1 Université de Lyon, INSA Lyon, MATEIS UMR5510, 7 avenue J Capelle, 69621 Villeurbanne Cedex, France
    Microsc Microanal 20:366-75. 2014
    ..The example studied here is hydrophilic mesoporous silica (MCM-41)...
  81. pmc XIAP-targeting drugs re-sensitize PIK3CA-mutated colorectal cancer cells for death receptor-induced apoptosis
    M Ehrenschwender
    Institute of Clinical Microbiology and Hygiene, University Hospital of Regensburg, Regensburg, Germany
    Cell Death Dis 5:e1570. 2014
    ..we investigated the molecular mechanisms underlying TRAIL resistance in CRC cells with a mutant PIK3CA (PIK3CA-mut) gene...
  82. pmc An essential role for chaperone-mediated autophagy in cell cycle progression
    Maimon E Hubbi
    a Vascular Program Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore, MD, USA
    Autophagy 11:850-1. 2015
    ..subunit [basic helix-loop-helix transcription factor]), which has a nontranscriptional role as an inhibitor of MCM (minichromosome maintenance complex component) helicase activity...
  83. pmc Cross-linking immunoprecipitation-MS (xIP-MS): Topological Analysis of Chromatin-associated Protein Complexes Using Single Affinity Purification
    Matthew M Makowski
    From the Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Geert Grooteplein 28, 6525 GA Nijmegen, The Netherlands
    Mol Cell Proteomics 15:854-65. 2016
    ..We then applied xIP-MS to the chromatin-associated cohesin (SMC1A/3), XRCC5/6 (Ku70/86), and MCM complexes, and we provide novel structural and biological insights into their architectures and molecular function...
  84. doi Role of Temperature and Pressure on the Multisensitive Multiferroic Dicyanamide Framework [TPrA][Mn(dca)3] with Perovskite-like Structure
    Juan M Bermúdez-García
    QuiMolMat Group, Department of Fundamental Chemistry, Faculty of Science and CICA, University of A Coruna, Campus A Coruña, 15071 A Coruna, Spain
    Inorg Chem 54:11680-7. 2015
    ..space group P4̅21c, to the high temperature polymorph II, with the centrosymmetric space group I4/mcm, and it implies ionic displacements, order-disorder phenomena, and a large and anisotropic thermal expansion (..
  85. pmc Myotubes derived from human-induced pluripotent stem cells mirror in vivo insulin resistance
    Salvatore Iovino
    Section on Integrative Physiology and Metabolism, Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215 Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02215
    Proc Natl Acad Sci U S A 113:1889-94. 2016
    ..muscle insulin resistance by differentiating iPS cells from individuals with mutations in the insulin receptor (IR-Mut) into functional myotubes and characterizing their response to insulin in comparison with controls...
  86. pmc Male Clients of Male Sex Workers in China: An Ignored High-Risk Population
    Lei Chen
    Department of STI Control, Guangdong Provincial Center for Skin Disease and STI Control, Guangzhou, China Department of Epidemiology, School of Public Health, University of California, Los Angeles, CA Department of HIV and STI Prevention and Control, Jiangsu Provincial Central for Disease Prevention and Control, Nanjing, China University of North Carolina, Project China, Guangzhou, China and Monitoring and Evaluation Unit, The Global Fund to Fight AIDS, Tuberculosis and Malaria, Geneva, Switzerland
    J Acquir Immune Defic Syndr 71:316-22. 2016
    ..among male sex workers, but no formal study has ever been conducted focusing on male clients of male sex workers (MCM)...
  87. doi Recent advances in mesoporous silica nanoparticles for antitumor therapy: our contribution
    Alejandro Baeza
    Departamento de Química Inorgánica y Bioinorgánica Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i 12, Plaza Ramon y Cajal s n, E 28040 Madrid, Spain
    Biomater Sci 4:803-13. 2016
    Since 2001, when our research group proposed for the first time MCM-41 as a drug release system, the scientific community has demonstrated a growing interest in mesoporous silica nanoparticles (MSNs) for their revolutionary potential in ..
  88. pmc Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation
    Tom D Deegan
    The Francis Crick Institute, Clare Hall Laboratory, South Mimms Herts, UK
    EMBO J 35:961-73. 2016
    The initiation of eukaryotic DNA replication requires the assembly of active CMG (Cdc45-MCM-GINS) helicases at replication origins by a set of conserved and essential firing factors...
  89. pmc HDAC10 promotes lung cancer proliferation via AKT phosphorylation
    Yiwei Yang
    Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Science and Technology, Tongji University, Shanghai 200092, P R China
    Oncotarget 7:59388-59401. 2016
    ..motif, R505L506L507C508V509A510L511, was identified as its nuclear localization signal (NLS), and a mutant (Mut-505-511) featuring mutations to A at each of its original R and L positions was found to be nuclear-localization ..
  90. ncbi Mine water supply assessment and evaluation of the system response to the designed demand in a desert region, central Saudi Arabia
    Yohannes Yihdego
    Snowy Mountains Engineering Corporation SMEC, Sydney, NSW, 2060, Australia
    Environ Monit Assess 188:619. 2016
    ..5 million cubic metre (MCM)/year) for 10 years...
  91. ncbi Role of KRAS mutation in predicting response, progression-free survival, and overall survival in irinotecan-refractory patients treated with cetuximab plus irinotecan for a metastatic colorectal cancer: Analysis of 281 individual data from published serie
    F Di Fiore
    University Hospital Gasthuisberg, Leuven, Belgium Inserm U775, Paris, France Ospedale Niguarda Ca Granda, Milan, Italy Institute of Pathology, Locarno, Switzerland Inserm U775, Paris, France University of Torino Medical School, Torino, Italy
    J Clin Oncol 26:4035. 2008
    ..Recent studies have shown that KRAS mutation (mut) confers resistance to anti-EGFR mAbs...
  92. ncbi Expression of human soluble gp190 in yeast Pichia pastoris
    Gang Li
    Department of Genetics and Eugenics, Chongqing Medical University, Chongqing 400016, China
    Yi Chuan Xue Bao 31:1182-9. 2004
    ..MD medium and PCR method were used for screening positive transformants. The transformants (His+ Mut+) containing multi-copy gene insertion were selected with increasing concentration of antibiotic G418 and induced ..
  93. pmc Mutations in subdomain B of the minichromosome maintenance (MCM) helicase affect DNA binding and modulate conformational transitions
    Elizabeth R Jenkinson
    Department of Biology and Technology Facility, University of York, York YO10 5YW, United Kingdom
    J Biol Chem 284:5654-61. 2009
    Minichromosome maintenance (MCM) proteins are believed to provide the replicative helicase activity in eukaryotes and archaea...
  94. pmc Extended release felodipine self-nanoemulsifying system
    Pradeep R Patil
    Department of Pharmaceutics, Poona College of Pharmacy, Erandwane, Bharati Vidyapeeth University, Pune, 411 038, Maharashtra State, India
    AAPS PharmSciTech 10:515-23. 2009
    ..SNES was formulated using varying amounts of Miglyol 840 (as an oil), Cremophor EL (as a surfactant), and Capmul MCM (as a co-surfactant). The SNES were characterized for turbidity, droplet size and in vitro FLD release...
  95. doi Gelsolin, but not its cleavage, is required for TNF-induced ROS generation and apoptosis in MCF-7 cells
    Qinxi Li
    The Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen, Fujian, China
    Biochem Biophys Res Commun 385:284-9. 2009
    ..Exogenous expression of gelsolin in this mutant cell line (Gel(mut)) restored the sensitivity to TNF-induced cell death and knock-down of gelsolin by siRNA conferred MCF-7 cells with ..
  96. doi Highly efficient luminescent hybrid materials covalently linking with europium(III) complexes via a novel fluorinated beta-diketonate ligand: synthesis, characterization and photophysical properties
    Biju Francis
    Chemical Sciences and Technology Division, National Institute for Interdisciplinary Science and Technology NIIST, CSIR, Thiruvananthapuram, 695 019, India
    Dalton Trans 39:8084-92. 2010
    ..was also constructed by linking the ternary europium(III) complex to the functionalized hexagonal mesoporous MCM-41 through the modified beta-diketonate ligand (SiBBPPF-Na)...
  97. pmc Impaired autophagy and delayed autophagic clearance of transforming growth factor β-induced protein (TGFBI) in granular corneal dystrophy type 2
    Seung Il Choi
    Corneal Dystrophy Research Institute and Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea
    Autophagy 8:1782-97. 2012
    ..Most notably, mutant TGFBI (mut-TGFBI) extensively colocalized with microtubule-associated protein 1 light chain 3β (MAP1LC3B, hereafter referred ..
  98. doi Photochemical trajectory modeling of ozone concentrations in Hong Kong
    H R Cheng
    Department of Environmental Engineering, School of Resources and Environmental Sciences, Wuhan University, China
    Environ Pollut 180:101-10. 2013
    ..A photochemical trajectory model coupled with Master Chemical Mechanism (MCM) was adapted to simulate the O3 concentrations during two O3 pollution episodes...
  99. pmc TP53 and let-7a micro-RNA regulate K-Ras activity in HCT116 colorectal cancer cells
    Carrie Luu
    Division of Surgical Oncology, Department of Surgery, City of Hope Comprehensive Cancer Center, Duarte, California, USA
    PLoS ONE 8:e70604. 2013
    ..To investigate the relationship between KRAS, TP53, and let-7a, we used HCT116 KRAS(mut) human colorectal cancer cells with four different genotypic modifications in TP53 (TP53(-/-), TP53(+/-), TP53(mut/+..
  100. doi Pathways enrichment analysis for differentially expressed genes in squamous lung cancer
    Liqiang Qian
    Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiaotong University, NO 241 Huaihai Road, Shanghai, 200030, China
    Pathol Oncol Res 20:197-202. 2014
    ..Cell cycle and metabolism were the mainly changed functions of SQLC cells. Meanwhile genes such as MCM, RFC, FEN1, and POLD may induce SQLC through DNA replication pathway, and genes such as PTTG1, CCNB1, CDC6, and ..
  101. doi Epidermal RelA specifically restricts contact allergen-induced inflammation and apoptosis in skin
    Snehlata Kumari
    Department of Dermatology, University of Cologne, Cologne, Germany
    J Invest Dermatol 134:2541-50. 2014
    ..We therefore mutated rela specifically in the epidermis of mice (RelA(E-MUT) mice). These mice show no inflammatory phenotype...

Research Grants66

  1. Anja Katrin Bielinsky; Fiscal Year: 2016
    ..Lessons learnt from simple organisms will be applied to the human system to understand the molecular pathways of cancer. ..
  2. James A Borowiec; Fiscal Year: 2014
    ..RPA selectively associates with the replication fork, by characterization of its interaction with the MCM complex...
  3. Functional analysis of Chk2 in DNA damage response
    Jun Hyun Kim; Fiscal Year: 2013
    ..large-scale phosphoproteomic screening has resulted in the finding of new Chk2 targets, minichromosome maintenance (MCM) that might be associated with Chk2 functions in S-phase...
  4. Genetic Analysis of MSM Protein Function in S. Pombe
    Susan L Forsburg; Fiscal Year: 2012
    ..Dysregulation of the genes encoding the conserved MCM helicase occurs in cancer cells, and mcm mutations are also linked to cancer development in mice...
  5. Yilun Liu; Fiscal Year: 2015
    ..Our studies allow us to conclude that RECQ4 is an integral component of the MCM replicative helicase complex participating in DNA replication in human cells...
  6. GENETIC AND MOLECULAR ANALYSIS OF YEAST DNA REPLICATION
    Robert A Sclafani; Fiscal Year: 2012
    ..Three specific aims are proposed. In aim #1, we will elucidate the function and regulation of the MCM DNA helicase by investigating both the N- terminal and C- terminal [unreadable] (Beta)-fingers and the N-terminal ..
  7. Kate E Coleman; Fiscal Year: 2015
    ..replication in S phase by the chromatin-loading of a DNA helicase known as the Mini-Chromosome Maintenance Complex (MCM) during G1 phase of the cell cycle...
  8. Regulation of MCM helicase loading and activation in the model system Saccharomyc
    Rebecca Ferguson; Fiscal Year: 2013
    ..The long-term objective of this proposal is to understand the regulation of MCM helicase function for eukaryotic chromosomal DNA replication using the model system Saccharomyces cerevisiae...
  9. The Role of the MCM2-7 Complex in the Replication Fork Processivity
    Anthony Schwacha; Fiscal Year: 2013
    ..As one of the few factors essential in both the initiation and elongation phases of DNA replication, defects in MCM activity can compromise the precision of DNA replication in multiple ways...
  10. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..structural feature in the ORC-Cdc6p complex that is similar in size to the presumptive replicative hexameric MCM helicase...
  11. Replication Initiation in Bacteria and Eukaryotes
    David Jeruzalmi; Fiscal Year: 2012
    ..Aim #3 exploits our recent identification of a unique bacterial MCM complex, which is more tractable, but should still provide a faithful model for its eukaryotic counterparts...
  12. Jack D Griffith; Fiscal Year: 2016
    ..attached to magnetic particles will be incubated with yeast cell extracts to load the pre-RC complex including Mcm and Cdc6 factors. Their structure will be probed by Western analysis and EM using nanoscale 'biopointers'...
  13. NICHOLAS R RHIND; Fiscal Year: 2015
    ..We propose that the probability of origin firing is regulated by the number of MCM complexes - the replicative helicase which establishes an origin as a site of replication initiation - loaded ..
  14. Role of Histone H2B Ubiquitylation in DNA Replication
    KELLY MIGUEL TRUJILLO; Fiscal Year: 2013
    ..I have discovered that the MCM helicase falls off the template in htb-K123R cells that cannot be ubiquitylated at the G1-S phase transition...
  15. Mark G Alexandrow; Fiscal Year: 2014
    ..Cdt1 stimulates the loading of Mini- Chromosome Maintenance (MCM) proteins at preRCs, and Geminin blocks this effect, but the molecular mechanisms behind this are unknown...
  16. Structural Studies of MCM Complex
    Xiaojiang S Chen; Fiscal Year: 2012
    Project Summary: Structural studies of MCM complex Minichromosome maintenance protein (MCM) complex functions as a helicase that unwinds DNA to provide template for DNA replication and plays a critical role in regulating cell growth...
  17. ERIC ENEMARK; Fiscal Year: 2016
    ..In human and other eukaryotic cells, the helicase engine is the hexameric MCM complex. The mechanisms that MCMs use to operate upon DNA are poorly understood at the molecular level...
  18. Structural Basis of Large T Helicase Function in SV40 DNA Replication
    Xiaojiang S Chen; Fiscal Year: 2013
    ..the helicase and cellular initiator proteins that consist of multiple initiator factors (such as Orc, Ctd1, Cdc6, MCM in eukaryotes, or DnaA/DnaC/DnaB in prokaryotes) to initiate DNA replication, i.e...
  19. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 2002
    ..The MCM proteins are a family of six related proteins that play a central role in the replication process in the yeast S...
  20. Role of Ddk Kinase in Regulation of DNA Replication
    Wei Jiang; Fiscal Year: 2006
    ..We will examine whether HsCdc7/HsDbf4 phosphorylation of MCM2 affects the formation or conformation of MCM heteromeric complexes and regulates the helicase activity of MCM complex(es)...
  21. Jeffrey S Souris; Fiscal Year: 2016
    ..invasive M-T staging of polyp/neoplastic disease in situ, we will synthesize and tri-functionalize biocompatible MCM-41 mesoporous silica nanoparticles (MSNs) for the targeted, endoscopically-traceable, delivery of conventional ..
  22. INBORN ERRORS--MOLECULAR ANALYSIS IN CULTURED CELLS
    Leon Rosenberg; Fiscal Year: 1990
    ..to cystathionine beta-synthase (CS) deficiency; methylmalonic acidemia, due to methylmalonyl CoA mutase apoenzyme (MUT) deficiency; hyperammonemia, due to ornithine transcarbamylase (OTC) deficiency; and propionic acidemia, due to ..
  23. Massimo Attanasio; Fiscal Year: 2016
    ..the effect of the inhibition of TLR-2/NF-?B signaling on the inflammatory infiltration and fibrosis in Glis2mut/mut mice kidneys by generating Tlr2-/-;Glis2mut/mut and Myd88flox/flox;Glis2mut/mut;KspCre double mutants and ..
  24. Gary M Kupfer; Fiscal Year: 2014
    ..Our data indicate that binding to a subset of the MCM subunits results in inhibition of replication complex assembly...
  25. VEGF and Skeletal Muscle Adaptation during Chronic Overload
    KIMBERLY ANN HUEY; Fiscal Year: 2011
    ..or mice overexpressing wild type Hsp27 (human form of Hsp25) (hspTG) or a non-phosphorylatable Hsp27 mutant (mut-hspTG), we will test the hypothesis that (p)Hsp25 is a critical mediator of the angiogenic, myogenic and/or anti-..
  26. Charles A Thornton; Fiscal Year: 2014
    ..end, we are proposing to develop RNase H-active antisense oligonucleotides (ASOs) targeting the mutant human DMPK {mut-hDMPK) mRNA...
  27. Resolution of Epithelial Cell Hyperplasia
    Yohannes Tesfaigzi; Fiscal Year: 2013
    ..Epithelial cell hyperplasia in response to an inflammatory response is accompanied by mucous cell metaplasia (MCM), the appearance of mucous cells in airways that are normally devoid of these cells...
  28. Mechanisms of CDX2 regulation of the IGF axis
    Duyen Dang; Fiscal Year: 2013
    ..Conversely, in colon cancers with MUT-p53, CDX2 has the opposite effect...
  29. KL4 Surfactant to Mitigate Radiation-Induced Lung Injury
    Robert Segal; Fiscal Year: 2013
    ..is evaluating its proprietary peptide-based synthetic KL4 surfactant (lucinactant) as a broad-spectrum, multi-use MCM against chemical, biological, radiological and nuclear threat agents targeting the lung...
  30. MYRON GOODMAN; Fiscal Year: 2014
    ..to transfer a molecule of RecA7ATP from its 3'- end to convert inactive pol V into mutagenically active pol V Mut. The properties of pol V Mut (UmuD'2C- RecA7ATP) are regulated through a biochemical cycle of polymerase ..
  31. Yohannes Tesfaigzi; Fiscal Year: 2016
    ..This phenotype is called mucous cell metaplasia (MCM)...
  32. MOLECULAR GENETIC STUDIES ON METHYLMALONYL COA MUTASE
    Fred Ledley; Fiscal Year: 1992
    ..clone for methylmalonyl CoA mutase from human liver and demonstrated that this clone can be used to transduce MCM activity into cultured cells by genetic transfer...
  33. Defining the importance of CD8+ T Cell Breadth in SIV/HIV protective immunity
    DAVID H O'CONNOR; Fiscal Year: 2013
    ..We have discovered that Mauritian cynomolgus macaques (MCM) infected with simian immunodeficiency virus (SIV), a virus that causes the same disease as HIV causes in humans, ..
  34. ROBERT LOUDEN MACDONALD; Fiscal Year: 2016
    ..to a dominant negative effect of the mutant subunit, 2) the mutation alters cortical and thalamic inhibition, 3) mut [unreadable]3 (P11S) subunits are reduced in mouse brain, and 4) the mutation causes altered transcriptional ..
  35. The Role of Osteoactivin in Osteoblast Development and Function
    Fayez F Safadi; Fiscal Year: 2013
    ..During the previous funding period and since the last submission, we established colonies of OA KO and OA mut. mice, and also generated transgenic (Tg) mice that over-express OA in bone. Preliminary data from OA KO, OA mut...
  36. Optimization of SWCNT/siRNA complex formulation for tumor accumulation
    Lynn Kirkpatrick; Fiscal Year: 2012
    ..Mutant KRAS (mut-KRAS) is the prototypical undruggable cancer target...
  37. MYRON GOODMAN; Fiscal Year: 2016
    ..is to transfer a molecule of RecA-ATP from its 32-end to convert inactive Pol V into mutagenically active Pol V Mut (UmuD22C-RecA-ATP)...
  38. Abstinence Reinforcement Therapy (ART) for Rural Veteran Smokers
    Patrick S Calhoun; Fiscal Year: 2013
    ..TC), a tele-medicine clinic for access to nicotine replacement (NRT), and mobile contingency management (mCM) or a control condition (i.e...
  39. Richard J H Smith; Fiscal Year: 2015
    ..e. low frequency hearing loss as seen with WFS1) (Hilgert et al Mut Res 2009)...
  40. MDEpiNet: MCM (Medical Countermeasures)
    Art Sedrakyan; Fiscal Year: 2012
    Summary This application proposes creation of a major innovative initiative/program on medical countermeasures (MCM) within the Medical Device Epidemiology Network (MDEpiNet)...
  41. JEFF JOSEPH SEKELSKY; Fiscal Year: 2015
    ..To better understand crossover control, we will test hypotheses for functions of a set of genes that encode MCM-like proteins essential for crossover formation, using a combination of genetic, biochemical, and cytological ..
  42. James W Lillard; Fiscal Year: 2014
    ..b CXCR3 interactions, JYANT Technologies has developed a novel mutant CXCR3 ligand-immunoglobulin fusion protein (mut-CXCL11-Ig) that lacks immunogenic, glycosaminoglycan (connective tissues)-binding sites but retains the ability to ..
  43. Patricia Cortes; Fiscal Year: 2014
    ..will address these issues while extending our findings that showed interaction of Rag2 with core histones and the MCM complex...
  44. 2D Silicon Transducer Compression Plates for Breast Ultrasound
    KAI ERIK THOMENIUS; Fiscal Year: 2010
    ..the development of a novel breast imaging device based on the use of silicon micro-machined ultrasound transducer (MUT) arrays placed on both upper and lower compression plates in a manner similar to mammography...
  45. THE MDEPINET MEDICAL COUNTER MEASURES STUDY
    Frederic S Resnic; Fiscal Year: 2012
    ..Because medical countermeasures (MCM) devices require rapid assessment and approval to ensure an effective public health response in the event of a ..
  46. Scott Kopetz; Fiscal Year: 2016
    ..and resistance to targeted therapy for CRC;and 3) conduct clinical trials of inhibitors of AKT, PI3K, and mut-KRAS signaling in patients with advanced CRC using patient tumor biopsy results to identify potential biomarkers ..
  47. Garth Powis; Fiscal Year: 2016
    ..Mutant KRAS (mut-KRAS) is the predominant oncogenic form of RAS, and is present in 17-25% of all human cancers where it plays a ..
  48. MCM Helicase as a Novel Target for Pancreatic Cancer Treatment
    Mark G Alexandrow; Fiscal Year: 2012
    ..The goal of this proposal is to explore the novel possibility that co-suppression of the replicative MCM helicase complex can enhance the anti-proliferative effects of chemotherapeutic agents used in pancreatic cancer ..
  49. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..1) Molecular characterization of mutants defective in dsRNA-mediated silencing (Mut-68, Mut-70, and Mut-91)...
  50. Roger Abounader; Fiscal Year: 2014
    ..tumor promoting properties by enhancing the half-life and oncogenic effects of gain-of-function p53 mutants (mut-p53)...
  51. FACTORS REGULATING TRANSPOSITION IN C. ELEGANS
    John Collins; Fiscal Year: 1992
    ..activity of Tc3, Tc4, and Tc5 has been detected only in genetic backgrounds containing the mutator mutation mut-2(r459). Genetic methods will be used to determine. if the activity of these elements is indeed mut-2-specific...
  52. Targeting mutant KRAS for cancer therapy
    Lynn Kirkpatrick; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Mutant KRAS (mut-KRAS) is the prototypical undruggable cancer target...
  53. Role of ATM in Radiosensitivity of Gliomas
    Chandan Guha; Fiscal Year: 2004
    ..t.) and U-138 (p53 mut) GBM cells resulting in enhanced radiosensitivity in vitro...
  54. ENDOTOXIN INDUCED MUCOUS CELL METAPLASIA
    Yohannes Tesfaigzi; Fiscal Year: 2003
    ..that Bcl-2 expression (a gene that inhibits apoptosis or programmed cell death) plays in mucous cell metaplasia (MCM)...
  55. Jonah B Sacha; Fiscal Year: 2014
    ..In contrast, Mauritian cynomolgus macaques (MCM), descendants from a population bottleneck event, exhibit simplified MHC genetics with only seven completely ..
  56. Canine model for assessing CNS access P-gp substrates
    KATRINA MEALEY; Fiscal Year: 2005
    The overall goal of the proposed research is to validate a P-glycoprotein (P-gp) deficient canine model [MDR1(mut/mut)] for delineating the role of P-gp in restricting access of therapeutic agents to the central nervous system (CNS)...
  57. PROTEIN PHOSPHATASE 2A AND TUMOR ANTIGENS IN CELL GROWTH
    Gernot Walter; Fiscal Year: 2004
    ..The main focus will be on identifying the substrate(s) of PP2A. Potential candidates are ORC, cdc6, MCM, cdc45, RPA, and DNA polymerase alpha, known to be involved in initiation...
  58. REGULATION OF MISMATCH REPAIR IN A MODEL E COLI SYSTEM
    ALICIA DOMBROSKI; Fiscal Year: 2001
    DESCRIPTION: The long-term goal of this project is to understand the regulatio of the mut S, mutL, and mut H DNA repair genes and the possible implications o this regulation to DNA repair, spontaneous and induced mutagenesis, and ..
  59. FISSION YEAST DNA REPLICATION ORGINS
    Joel Huberman; Fiscal Year: 2001
    ..ORC binding appears necessary for the subsequent binding of Cdc6p, the Mcm family of proteins, and perhaps other proteins at the beginning of G1 phase...
  60. EFFECTS OF 03/ENDOTOXIN COEXPOSURE ON NASAL EPITHELIUM
    James Wagner; Fiscal Year: 2000
    ..Ozone exposure causes inflammation that is followed by mucous cell metaplasia (MCM) which is the appearance of mucous secreting cells in regions of nasal epithelium normally devoid of secretory ..
  61. MOOD AND MEMORY
    JOHN KIHLSTROM; Fiscal Year: 1990
    ..autobiographical memory designs to explore the phenomena of mood- dependent memory (MDM) and mood-congruent memory (MCM) and resource allOcation effects...
  62. Development of Medical Countermeasures to Mitigate or Treat GI-ARS
    THOMAS RIVENBURG; Fiscal Year: 2013
    The contracts awarded under this initiative will advance the development of candidate medical countermeasures (MCM) that can mitigate or treat gastrointestinal (GI) syndrome of the acute radiation syndrome (GI-ARS) and increase survival...