MUT

Summary

Gene Symbol: MUT
Description: methylmalonyl-CoA mutase
Alias: MCM, methylmalonyl-CoA mutase, mitochondrial, methylmalonyl Coenzyme A mutase, methylmalonyl-CoA isomerase, mutant methylmalonyl CoA mutase, truncated methylmalonyl CoA mutase
Species: human

Top Publications

  1. doi Minichromosome maintenance protein expression in benign nevi, dysplastic nevi, melanoma, and cutaneous melanoma metastases
    Alan S Boyd
    Department of Medicine Dermatology, Vanderbilt University, Nashville, Tennessee, USA
    J Am Acad Dermatol 58:750-4. 2008
  2. doi Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
    Tóshiko Takahashi-Íñiguez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
    Biochem Biophys Res Commun 404:443-7. 2011
  3. pmc Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
    D Sean Froese
    Structural Genomics Consortium, University of Oxford OX3 7DU, United Kingdom
    J Biol Chem 285:38204-13. 2010
  4. ncbi Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
  5. pmc Loss of allostery and coenzyme B12 delivery by a pathogenic mutation in adenosyltransferase
    Michael Lofgren
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0600, USA
    Biochemistry 50:5790-8. 2011
  6. ncbi Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
  7. pmc Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes
    L Folgueira
    Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 70:223-31. 1996
  8. pmc Liver-directed recombinant adeno-associated viral gene delivery rescues a lethal mouse model of methylmalonic acidemia and provides long-term phenotypic correction
    Nuria Carrillo-Carrasco
    Organic Acid Research Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892, USA
    Hum Gene Ther 21:1147-54. 2010
  9. ncbi Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
    E L LeCluyse
    Department of Pharmacology, Toxicology, and Therapeutics, Center for Environmental and Occupational Health, Kansas University Medical Center, Kansas City, Kansas, USA
    Toxicol In Vitro 14:101-15. 2000
  10. doi Cobalamin deficiency results in an abnormal increase in L-methylmalonyl-co-enzyme-A mutase expression in rat liver and COS-7 cells
    Motoyuki Nakao
    Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka 599 8531, Japan
    Br J Nutr 101:492-8. 2009

Detail Information

Publications319 found, 100 shown here

  1. doi Minichromosome maintenance protein expression in benign nevi, dysplastic nevi, melanoma, and cutaneous melanoma metastases
    Alan S Boyd
    Department of Medicine Dermatology, Vanderbilt University, Nashville, Tennessee, USA
    J Am Acad Dermatol 58:750-4. 2008
    Minichromosome maintenance (MCM) proteins are a recently elucidated group of polypeptides intimately involved in DNA replication and appreciable only in cycling cells...
  2. doi Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
    Tóshiko Takahashi-Íñiguez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
    Biochem Biophys Res Commun 404:443-7. 2011
    ..examined the influence of human MMAA protein on the kinetics of the reaction catalyzed by methylmalonyl-CoA mutase (MCM) by testing both purified recombinant proteins in vitro...
  3. pmc Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
    D Sean Froese
    Structural Genomics Consortium, University of Oxford OX3 7DU, United Kingdom
    J Biol Chem 285:38204-13. 2010
    ..Cbl) is essential to the function of two human enzymes, methionine synthase (MS) and methylmalonyl-CoA mutase (MUT)...
  4. ncbi Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
    ..These studies provide insights into the energetics of interaction between the radical enzyme methylmalonyl-CoA mutase and MeaB, which are discussed...
  5. pmc Loss of allostery and coenzyme B12 delivery by a pathogenic mutation in adenosyltransferase
    Michael Lofgren
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0600, USA
    Biochemistry 50:5790-8. 2011
    ..and an escort that delivers the product, adenosylcobalamin (AdoCbl or coenzyme B(12)), to methylmalonyl-CoA mutase (MCM), resulting in holoenzyme formation. Failure to assemble holo-MCM leads to methylmalonic aciduria...
  6. ncbi Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
    ....
  7. pmc Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes
    L Folgueira
    Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 70:223-31. 1996
    ..In addition, cotransfection of a negative dominant molecule of PKC-zeta (PKC-zeta mut) with NF-kappa B-dependent reporter genes selectively inhibits the HIV- but not phorbol myristate acetate- or ..
  8. pmc Liver-directed recombinant adeno-associated viral gene delivery rescues a lethal mouse model of methylmalonic acidemia and provides long-term phenotypic correction
    Nuria Carrillo-Carrasco
    Organic Acid Research Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892, USA
    Hum Gene Ther 21:1147-54. 2010
    ..disorder caused by a deficiency of the ubiquitously expressed mitochondrial enzyme, methylmalonyl-CoA mutase (MUT)...
  9. ncbi Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
    E L LeCluyse
    Department of Pharmacology, Toxicology, and Therapeutics, Center for Environmental and Occupational Health, Kansas University Medical Center, Kansas City, Kansas, USA
    Toxicol In Vitro 14:101-15. 2000
    ..Hepatocytes cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) or modified Chee's medium (MCM) maintained similar levels of total GST protein regardless of the matrix configuration or corresponding cell ..
  10. doi Cobalamin deficiency results in an abnormal increase in L-methylmalonyl-co-enzyme-A mutase expression in rat liver and COS-7 cells
    Motoyuki Nakao
    Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka 599 8531, Japan
    Br J Nutr 101:492-8. 2009
    ..study was to examine the effects of cobalamin (Cbl) on the activity and expression of L-methylmalonyl-CoA mutase (MCM) in rat liver and cultured COS-7 cells...
  11. ncbi Molecular analysis and heterologous expression of the gene encoding methylmalonyl-coenzyme A mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
    W Zhang
    Department of Microbiology, Shanghai Institute of Plant Physiology, Academia Sinica, P R China
    Appl Biochem Biotechnol 82:209-25. 1999
    ..A (CoA) into methylmalonyl-CoA, catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM), represents an important source of building blocks for rifamycin SV biosynthesis...
  12. pmc Primary structure and activity of mouse methylmalonyl-CoA mutase
    M F Wilkemeyer
    Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030
    Biochem J 271:449-55. 1990
    Methylmalonyl-CoA mutase (MCM) is an adenosylcobalamin-dependent enzyme that catalyses isomerization between methylmalonyl-CoA and succinyl-CoA (3-carboxypropionyl-CoA)...
  13. ncbi Effect of fetuin, a TGFbeta antagonist and pentoxifylline, a cytokine antagonist on hepatic stellate cell function and fibrotic parameters in fibrosis
    Monica Verma-Gandhu
    Department of Pharmacology Faculty of Medicine Dalhousie University, Canada
    Eur J Pharmacol 572:220-7. 2007
    We have previously shown that monocyte conditioned medium (MCM) from patients with liver fibrosis stimulated proliferation of hepatic stellate cells (HSCs), the major cell involved in hepatic fibrosis...
  14. ncbi Initiating DNA synthesis: from recruiting to activating the MCM complex
    M Lei
    Dept of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cell Sci 114:1447-54. 2001
    ..A key component of the pre-RC is the hexameric MCM complex, which is also the presumed helicase of the growing forks...
  15. pmc Genomic structure of murine methylmalonyl-CoA mutase: evidence for genetic and epigenetic mechanisms determining enzyme activity
    M F Wilkemeyer
    Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030
    Biochem J 296:663-70. 1993
    Methylmalonyl-CoA mutase (MCM) is a nuclear-encoded mitochondrial matrix enzyme...
  16. pmc Adenoviral-mediated correction of methylmalonyl-CoA mutase deficiency in murine fibroblasts and human hepatocytes
    Randy J Chandler
    National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Med Genet 8:24. 2007
    ..by deficiency of the mitochondrial localized, 5'deoxyadenosylcobalamin dependent enzyme, methylmalonyl-CoA mutase (MUT)...
  17. ncbi Mutation and haplotype analyses of the MUT gene in Japanese patients with methylmalonic acidemia
    Osamu Sakamoto
    Department of Pediatrics, Tohoku University School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai, 980 8574, Japan
    J Hum Genet 52:48-55. 2007
    Methylmalonic acidemia (MMA) is caused by a deficiency in the activity of L: -methylmalonyl-CoA mutase (MCM), a vitamin B12 (or cobalamin, Cbl)-dependent enzyme...
  18. ncbi Cloning, expression and sequence analysis of the genes encoding the heterodimeric methylmalonyl-CoA mutase of Porphyromonas gingivalis W50
    C A Jackson
    Biochemistry and Molecular Biology Unit, School of Dental Science, University of Melbourne, Australia
    Gene 167:127-32. 1995
    Two genes that encode methylmalonyl-CoA mutase (MCM) have been characterised in Porphyromonas gingivalis W50 (Pg)...
  19. pmc Adenovirus-mediated gene delivery rescues a neonatal lethal murine model of mut(0) methylmalonic acidemia
    Randy J Chandler
    Genetic Diseases Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 19:53-60. 2008
    ..MMA), an autosomal recessive metabolic disorder, is most often caused by mutations in methylmalonyl-CoA mutase (MUT)...
  20. ncbi Identification of the human and bovine ATP:Cob(I)alamin adenosyltransferase cDNAs based on complementation of a bacterial mutant
    Nicole A Leal
    Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 278:9227-34. 2003
    In humans, deficiencies in coenzyme B12-dependent methylmalonyl-CoA mutase (MCM) lead to methylmalonyl aciduria, a rare disease that is often fatal in newborns...
  21. doi Theoretical analysis of the diradical nature of adenosylcobalamin cofactor-tyrosine complex in B12-dependent mutases: inspiring PCET-driven enzymatic catalysis
    Pawel M Kozlowski
    Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, USA
    J Phys Chem B 114:5928-39. 2010
    ..In particular, methylmalonyl-CoA mutase (MCM) and glutamate mutase (GLM) enzymes have been studied; in the case of MCM, the significance of the Y89 residue has ..
  22. ncbi Experimental study on mechanism and rarity of metastases in skeletal muscle
    Chenghua Luo
    Department of General Surgery, 301 Hospital, Beijing 100853, China
    Chin Med J (Engl) 115:1645-9. 2002
    ..To investigate the reasons for the rarity of metastases in skeletal muscle...
  23. pmc Association of germline mutations in the fumarate hydratase gene and uterine fibroids in women with hereditary leiomyomatosis and renal cell cancer
    Laveta Stewart
    Division of Cancer Epidemiology and Genetics, Genetic Epidemiology Branch, National Cancer Institute, National Institutes of Health, Rockville, MD 20892 7231, USA
    Arch Dermatol 144:1584-92. 2008
    ..To investigate the risk of uterine fibroids and other reproductive risk factors in women with hereditary leiomyomatosis and renal cell cancer (HLRCC)...
  24. doi Comparing the General Practice Research Database and the UK Epilepsy and Pregnancy Register as tools for postmarketing teratogen surveillance: anticonvulsants and the risk of major congenital malformations
    Rachel A Charlton
    Department of Pharmacy and Pharmacology, University of Bath, Bath, UK
    Drug Saf 34:157-71. 2011
    ....
  25. ncbi [Couples' knowledge and attitudes regarding the practice of family planning in the jungle of Peru]
    Doris de la Cruz-Peñarán
    Instituto Nacional de Salud Pública INSP, Avenida Universidad 655, colonia Santa María, Ahuacatitlán 62508 Cuernavaca, Morelos, Mexico
    Salud Publica Mex 45:461-71. 2003
    ..To assess the association of knowledge and attitudes about family planning (FP) with its practice in couples of the Peruvian jungle region...
  26. pmc Analysis of genomic instability in Li-Fraumeni fibroblasts with germline p53 mutations
    P K Liu
    Division of Laboratory Medicine, Department of Tumor Biology, The University of Texas MD Anderson Cancer Center, Houston 77030, USA
    Oncogene 12:2267-78. 1996
    ..We used p53(wt/mut) and p53(mut/-) LFS fibroblasts, and p53(-/-) non-LFS cells...
  27. pmc Endothelial cell-derived lipase mediates uptake and binding of high-density lipoprotein (HDL) particles and the selective uptake of HDL-associated cholesterol esters independent of its enzymic activity
    Juliane G Strauss
    Institute of Molecular Biology, Biochemistry and Microbiology, University of Graz, Harrachgasse 21, A 8010 Graz, Austria
    Biochem J 368:69-79. 2002
    ..by the finding that, in contrast with EDL, the amount of cell-surface-bound enzymically inactive mutant EDL (MUT-EDL) was not reduced in the presence of HDL and foetal calf serum...
  28. doi Flavin adenine dinucleotide status and the effects of high-dose riboflavin treatment in short-chain acyl-CoA dehydrogenase deficiency
    Bianca T van Maldegem
    Department of Pediatrics, University of Amsterdam, Amsterdam, The Netherlands
    Pediatr Res 67:304-8. 2010
    ..in a prospective open-label cohort study involving 16 patients with SCADD, subdivided into mutation/mutation (mut/mut), mutation/variant (mut/var), and variant/variant (var/var) genotype groups...
  29. ncbi Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris
    Ramon Ramon
    Departament d Enginyeria Quimica, ETSE, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    J Biotechnol 130:39-46. 2007
    ..Remarkably, a different substrate consumption behaviour was observed depending on the host's phenotype (Mut(+) or Mut(s)) in batch cultures: when the methanol assimilation capacity is genetically reduced (Mut(s) phenotype), ..
  30. pmc Involvement of toll-like receptor 4 in alveolar bone loss and glucose homeostasis in experimental periodontitis
    K Watanabe
    Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612 7212, USA
    J Periodontal Res 46:21-30. 2011
    ..We therefore hypothesized that mice with periodontitis and a TLR4 loss-of-function (LOF) mutation fed a high-fat (HF) diet would develop improved glucose homeostasis compared with wild-type (WT) animals with periodontitis fed a HF diet...
  31. ncbi Long-lasting effects of chronic ozone exposure on rat nasal epithelium
    J R Harkema
    Department of Pathology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA
    Am J Respir Cell Mol Biol 20:517-29. 1999
    ..We have recently reported that long-term inhalation exposure to ozone causes mucous-cell metaplasia (MCM) in the surface epithelium lining the nasal airways of F344 rats...
  32. ncbi T-->G or T-->A mutation introduced in the branchpoint consensus sequence of intron 4 of lecithin:cholesterol acyltransferase (LCAT) gene: intron retention causing LCAT deficiency
    M Li
    Atherosclerosis Specialty Laboratory, Department of Pathology and Laboratory Medicine, St Paul s Hospital and University of British Columbia, 1081 Burrard Street, Vancouver, British Columbia, Canada
    Biochim Biophys Acta 1391:256-64. 1998
    ..e., LCAT Int-4 MUT-1 (T-->G) and LCAT Int-4 MUT-2 (T-->A)...
  33. ncbi The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts
    M A Madine
    Wellcome CRC Institute, Cambridge, UK
    Curr Biol 5:1270-9. 1995
    A complex of MCM proteins is implicated in ensuring that DNA replicates only once in each cell cycle, by 'replication licensing'...
  34. ncbi Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus
    Floriana Carpentieri
    Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via P Castellino 111, Napoli 80131, Italy
    J Biol Chem 277:12118-27. 2002
    Mini-chromosome Maintenance (MCM) proteins play an essential role in both initiation and elongation phases of DNA replication in Eukarya...
  35. pmc Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection
    Saira Sarfraz
    1Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
    BMC Microbiol 8:133. 2008
    ..and normal histology (n = 5) were analyzed by immunohistochemistry using antibodies against proliferation marker Mcm-2, G1 phase marker Cyclin D1, S phase marker Cyclin A, cell cycle regulators p21 (CDK inhibitor) and p53 (tumor ..
  36. ncbi [Histologic study of local infiltration of spinal bone giant cell tumor and chordoma]
    Jie Lan
    Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China
    Zhonghua Wai Ke Za Zhi 46:1808-11. 2008
    ..To study the local infiltration length of spinal bone giant cell tumor and chordoma in various para-tumorous tissues...
  37. ncbi Functional localization and competition between the androgen receptor and T-cell factor for nuclear beta-catenin: a means for inhibition of the Tcf signaling axis
    David J Mulholland
    The Prostate Centre, Jack Bell Research, Vancouver, BC, Canada V6H 3Z6
    Oncogene 22:5602-13. 2003
    ..Transient transfections in SW480 cells (APC(mut/mut)) showed that this mode of repression is functionally independent of APC-mediated beta-catenin ubiquitination...
  38. pmc Virulence variation among isolates of western equine encephalitis virus in an outbred mouse model
    Christopher H Logue
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA
    J Gen Virol 90:1848-58. 2009
    ..f.u. of each virus. Two WEEV strains, McMillan (McM) and Imperial 181 (IMP), were the most divergent of the six in genome sequence; McM caused 100 % mortality by 5 ..
  39. ncbi Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II
    Reiko Matsui
    Vascular Biology Unit, Whitaker Cardiovascular Institute and Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118 2393, USA
    Circulation 112:257-63. 2005
    ..We determined the hypertensive and vascular hypertrophic response to Ang II in G6PD-deficient mice...
  40. ncbi Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein
    Biagio Pucci
    Istituto di Biochimica delle Proteine, Consiglio Nazionale Ricerche, Via P Castellino, 111, 80131 Napoli, Italy
    J Biol Chem 282:12574-82. 2007
    Mini-chromosome maintenance (MCM) proteins form ring-like hexameric complexes that are commonly believed to act as the replicative DNA helicase at the eukaryotic/archaeal DNA replication fork...
  41. pmc Cloning and characterization of mouse intestinal MUC3 mucin: 3' sequence contains epidermal-growth-factor-like domains
    L L Shekels
    Department of Medicine, University of Minnesota and VA Medical Center, Minneapolis, MN 55417, USA
    Biochem J 330:1301-8. 1998
    ..We report here the cloning of a mouse caecal mucin (MCM). Amino acid analysis of purified MCM revealed a high content of serine (10.8%) and threonine (25.1%)...
  42. doi Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
    Alison D Walters
    Department of Biology Area 5, PO Box 373, University of York, York YO10 5YW, UK
    Biochem Soc Trans 37:1-6. 2009
    ..Key proteins involved in the initiation and elongation phases of DNA replication include the MCM (minchromosome maintenance) proteins, MCM2-MCM7, a family of six related proteins believed to act as the ..
  43. doi Accelerated oxidation of epinephrine by silica nanoparticles
    Zhimin Tao
    Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA
    Langmuir 25:10183-8. 2009
    We have measured the influence of mesoporous silica (MCM-41 and SBA-15) nanoparticles and dense silica nanoparticles on epinephrine oxidation, a pH-dependent reaction, whose rate is small in acidic or neutral solutions but much greater ..
  44. ncbi Improvement of dissolution rate of piroxicam by inclusion into MCM-41 mesoporous silicate
    V Ambrogi
    Dipartimento di Chimica e Tecnologia del Farmaco, Via del Liceo 1, Perugia, Italy
    Eur J Pharm Sci 32:216-22. 2007
    The aim of the present paper was the use of mesoporous silicate MCM-41 to increase the dissolution rate of piroxicam, a non-steroidal anti-inflammatory drug-class II of the Biopharmaceutic Classification System...
  45. pmc Calcicludine binding to the outer pore of L-type calcium channels is allosterically coupled to dihydropyridine binding
    Xianming Wang
    Department of Cellular and Molecular Physiology, The Penn State Milton S Hershey College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 46:7590-8. 2007
    ..segment of repeat III were sequentially changed to alanine in groups of three, resulting in the mutant channels, Mut-A, Mut-B, and Mut-C. Mut-A, whose substitutions are proximal to IIIS5, exhibits a 4...
  46. ncbi Study on the indoor volatile organic compound treatment and performance assessment with TiO2/MCM-41 and TiO2/quartz photoreactor under ultraviolet irradiation
    Chia Wei Tsai
    Department of Environmental Engineering, National I Lan University, Taiwan, Republic of China
    J Air Waste Manag Assoc 58:1266-73. 2008
    ..activated by visible irradiation and packed with TiO2/quartz or TiO2/mobile catalytic material number 41 (MCM-41)...
  47. ncbi Immunohistochemical evaluation of metallothionein, Mcm-2 and Ki-67 antigen expression in tumors of the adrenal cortex
    Artur Szajerka
    Second Department of General and Oncological Surgery, Medical University, M Curie Sklodowska Street 66, Poland
    Anticancer Res 28:2959-65. 2008
    The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these ..
  48. ncbi The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin
    P Romanowski
    Wellcome CRC Institute, Cambridge
    Curr Biol 6:1416-25. 1996
    The origin recognition complex (ORC) and the minichromosome maintenance (MCM) protein complex were initially discovered in yeast and shown to be essential for DNA replication...
  49. doi Induction of glioma apoptosis by microglia-secreted molecules: The role of nitric oxide and cathepsin B
    So Young Hwang
    Department of Physiology and Biophysics, and Center for Advanced Medical Education by BK21 Project, Inha University, College of Medicine, Nam Ku, Incheon 402 751, Korea
    Biochim Biophys Acta 1793:1656-68. 2009
    ..Microglia conditioned culture medium (MCM) actively stimulated apoptotic death of glioma cells, and the effects of MCM prepared from LPS- or IFN-gamma-..
  50. doi Determination of very low levels of gold and palladium in wastewater and soil samples by atomic absorption after preconcentration on modified MCM-48 and MCM-41 silica
    H Ebrahimzadeh
    Department of Chemistry, Shahid Beheshti University, G C, Evin, Tehran, Iran
    Talanta 81:1183-8. 2010
    ..Extraction was investigated on adsorbents prepared by grafting aminopropyl on the surface of MCM-41 and MCM-48 mesoporous silica...
  51. ncbi The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep
    G Cringoli
    Dipartimento di Patologia e Sanita Animale, Facolta di Medicina Veterinaria, Universita degli Studi di Napoli Federico II, CREMOPAR Regione Campania, Via Della Veterinaria 1, 80137 Napoli, Italy
    Vet Parasitol 123:121-31. 2004
    ..For each McMaster slide, the GI strongyle and D. dendriticum egg counts were performed under one grid (McM 0.15 ml), two grids (McM 0.3 ml), one chamber (McM 0.5 ml), and both chambers (McM 1.0 ml)...
  52. doi Novel and existing mutations in the tyrosine kinase domain of the epidermal growth factor receptor are predictors of optimal resectability in malignant peritoneal mesothelioma
    Jason M Foster
    Division of Surgical Oncology, Creighton University Medical Center, Creighton University, Omaha, NE 68131, USA
    Ann Surg Oncol 16:152-8. 2009
    ..and their tumors were probed for point mutations in the catalytic TK domain of epidermal growth factor receptor (mut+)...
  53. ncbi Photophysical Properties of Lanthanide Hybrids Covalently Bonded To Functionalized MCM-41 by Modified Aromatic Carboxylic Acids
    Ying Li
    Department of Chemistry, Tongji University, Shanghai, 200092, People s Republic of China
    J Fluoresc 19:191-201. 2009
    b>MCM-41 mesoporous silica has been functionalized with aromatic carboxylic acids salicylic acid (Sal) and 2-hydroxyl-3-methylbenzoic acid (HMBA) through co-condensation approach of tetraethoxysilane (TEOS) in the presence of the ..
  54. ncbi Omeprazole treatment of Korean patients: effects on gastric pH and gastrin release in relation to CYP2C19 geno- and phenotypes
    Hyung Keun Roh
    Department of Laboratory Medicine, Division of Clinical Pharmacology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Basic Clin Pharmacol Toxicol 95:112-9. 2004
    ..patients with acid related disease were genotyped for CYP2C19 by allele specific PCR (wt/wt, CYP2C19*1/*1; wt/mut, CYP2C19*1/*2 or *1/*3; mut/mut, CYP2C19*2/*2, *2/*3 or *3/*3)...
  55. pmc Specific targeting to B cells by lipid-based nanoparticles conjugated with a novel CD22-ScFv
    Kristin Loomis
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Exp Mol Pathol 88:238-49. 2010
    ..Therefore, we generated a mutant of the targeting segment of HA22 (a CD22 scFv) to increase its soluble expression (mut-HA22), and conjugated it to the surface of sonicated liposomes to generate immunoliposomes (mut-HA22-liposomes)...
  56. pmc A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae
    Bo Huang
    Department of Molecular Biology, Umea University, 901 87 Umea, Sweden
    RNA 14:2183-94. 2008
    ..tRNA(mcm5s2UUU Lys), and tRNA(mcm5s2UUG Gln) 3' of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U)...
  57. pmc The Nrf2-inducible antioxidant defense in astrocytes can be both up- and down-regulated by activated microglia:Involvement of p38 MAPK
    Fernando Correa
    Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, Sweden
    Glia 59:785-99. 2011
    The effects of microglia-conditioned medium (MCM) on the inducible Nrf2 system in astrocyte-rich cultures were investigated by determination of glutathione (GSH) levels, γglutamylcysteine ligase (γGCL) activity, the protein levels of ..
  58. pmc Immunohistochemical estimation of cell cycle phase in laryngeal neoplasia
    P Chatrath
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 2XZ, UK
    Br J Cancer 95:314-21. 2006
    ..Antibodies against the markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67, and putative markers of cell cycle phase, cyclin D1 (G1-phase), cyclin A (S-phase), cyclin B1 (G2-..
  59. doi Effects of lipophilic components on the compatibility of lipid-based formulations with hard gelatin capsules
    Feng Jing Chen
    Boehringer Ingelheim Pharmaceuticals Inc, 900 Ridgebury Road, Ridgefield, Connecticut 06877, USA
    J Pharm Sci 99:128-41. 2010
    ..1) and an additional lipophilic excipient (Capmul MCM) significantly affected the activity of PG in the fills and the equilibrium of PG between capsule shells and fills...
  60. ncbi Nuclear envelope alterations in fibroblasts from LGMD1B patients carrying nonsense Y259X heterozygous or homozygous mutation in lamin A/C gene
    Antoine Muchir
    INSERM U582, Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, 75013, Paris, France
    Exp Cell Res 291:352-62. 2003
    ..limb-girdle muscular dystrophy and from her deceased newborn grandchild carrying, respectively, a heterozygous (+/mut) and a homozygous (mut/mut) nonsense Y259X mutation...
  61. ncbi Cdc6 is a rate-limiting factor for proliferative capacity during HL60 cell differentiation
    Laura R Barkley
    Wolfson Institute for Biomedical Research, University College London, The Cruciform Building, Gower Street, London, WC1E 6BT, UK
    Exp Cell Res 313:3789-99. 2007
    ..Repression of origin licensing through down-regulation of the MCM licensing factors (Mcm2-7) is emerging as a ubiquitous route for lowering proliferative capacity as metazoan cells ..
  62. doi Dynamics of aqueous binary glass-formers confined in MCM-41
    Khalid Elamin
    Department of Applied Physics, Chalmers University of Technology, SE 412 96 Goteborg, Sweden
    Phys Chem Chem Phys 17:12978-87. 2015
    ..of propylene glycol (PG) and propylene glycol monomethyl ether (PGME) confined in 21 Å pores of the silica matrix MCM-41 C10 in wide frequency (10(-2)-10(6) Hz) and temperature (130-250 K) ranges...
  63. ncbi Functional analyses of mouse ASK, an activation subunit for Cdc7 kinase, using conditional ASK knockout ES cells
    Nobuyuki Yamashita
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    Genes Cells 10:551-63. 2005
    ..Further characterization reveals that motif-N is required for the maximum phosphorylation of MCM in cells, whereas the autophosphorylation activity of Cdc7 is not significantly affected by its loss...
  64. pmc Amphiregulin-dependent mucous cell metaplasia in a model of nonallergic lung injury
    Nicholas D Manzo
    Division of Pulmonary, Allergy and Critical Care, Duke University Medical Center, Durham, North Carolina, USA
    Am J Respir Cell Mol Biol 47:349-57. 2012
    ..Defects in this response can result in airway remodeling, such as mucus cell metaplasia (MCM), commonly seen in patients with chronic lung disease...
  65. doi Adsorption, kinetic and thermodynamic studies for manganese extraction from aqueous medium using mesoporous silica
    Salah Ali Mahgoub Idris
    Chemistry Department, Faculty of Science, University of Omar Al Mukhtar, Tobruk, Libya Electronic address
    J Colloid Interface Sci 440:84-90. 2015
    ..The surface area of MCM-41 and diethylenetriamine functionalized-MCM-41 used in this study were 760 and 318 m(2) g(-1) (N2 adsorption)...
  66. ncbi n- and isoalkane adsorption mechanisms on zeolite MCM-22
    Joeri F M Denayer
    Dienst Chemische Ingenieurstechniek, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
    J Phys Chem B 110:8551-8. 2006
    ..properties (Henry constants, adsorption enthalpy, and entropy) of linear and branched alkanes (C3-C8) on zeolite MCM-22 were determined using the chromatographic technique at temperatures between 420 and 540 K...
  67. pmc MCM proteins are associated with RNA polymerase II holoenzyme
    K Yankulov
    Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Mol Cell Biol 19:6154-63. 1999
    ..These results suggest a new function for MCM proteins as components of the Pol II transcriptional apparatus.
  68. pmc Germline mitochondrial DNA mutations aggravate ageing and can impair brain development
    Jaime M Ross
    Department of Neuroscience, Karolinska Institutet, Retzius vag 8, 171 77 Stockholm, Sweden
    Nature 501:412-5. 2013
    ..lead to anticipation of reduced fertility in mice that are heterozygous for the mtDNA mutator allele (PolgA(wt/mut)) and aggravate premature ageing phenotypes in mtDNA mutator mice (PolgA(mut/mut))...
  69. ncbi Immunohistochemistry of DNA mismatch repair enzyme MSH2 is not correlated with prognostic data from endometrial carcinomas
    Andreas Schröer
    Department of Gynecology and Obstetrics, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Anticancer Res 29:4833-7. 2009
    The human Mut-S-homolog-2 (MSH2) is part of the DNA mismatch repair system (MMR). Mutations in genes of the MMR are a predisposition to hereditary non-polyposis colorectal cancer (HNPCC)...
  70. doi Photoreduction of iron(III) to iron(0) nanoparticles for simultaneous hydrogen evolution in aqueous solution
    Chuan Jun Wang
    Key Laboratory of Photochemical Conversion and Optoelectronic Materials and HKU CAS Joint Laboratory on New Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190 P R China, Fax 86 10 6255 4670
    ChemSusChem 7:1924-33. 2014
    ..If inorganic support materials such as NH2 -MCM-41 or reduced graphene oxide were introduced, dispersed nanoparticles with different sizes and shapes were ..
  71. pmc Generation of mature monocyte-derived dendritic cells in the presence of heparin and monocyte conditioned medium: phenotypic and functional comparison
    Nowruz Delirezh
    Dept of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran
    Iran Biomed J 15:79-84. 2011
    ..We comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (MCM), with heparin for their ability to promote uniformly mature DC that elicit T cell responses.
  72. pmc Multiple functions for Mcm2-7 ATPase motifs during replication initiation
    Sukhyun Kang
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 55:655-65. 2014
    The Mcm2-7 replicative helicase is central to all steps of eukaryotic DNA replication. The hexameric ring of Mcm subunits forms six essential ATPases whose contributions to replication initiation remain unclear...
  73. pmc MCM2-7 proteins are essential components of prereplicative complexes that accumulate cooperatively in the nucleus during G1-phase and are required to establish, but not maintain, the S-phase checkpoint
    K Labib
    ICRF Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Mol Biol Cell 12:3658-67. 2001
    ..We show that, although mitosis does indeed occur in the absence of replication if MCM proteins are degraded during G1-phase, anaphase is prevented if MCMs are degraded during S-phase...
  74. pmc Multicomponent smoking cessation treatment including mobile contingency management in homeless veterans
    Vickie L Carpenter
    Durham Veterans Affairs Medical Center, 508 Fulton St 116 B, Durham, NC 27705
    J Clin Psychiatry 76:959-64. 2015
    ..This open pilot study builds on a web-based CM approach by evaluating a smartphone-based application for CM named mobile CM (mCM). The study was conducted from January 1, 2013-April 15, 2014.
  75. ncbi Peroxidase catalytic cycle of MCM-41-entrapped microperoxidase-11 as a mechanism for phenol oxidation
    Juliana C Araujo
    Centro Interdisciplinar de Investigação Bioquímica CIIB Universidade de Mogi das Cruzes UMC, Mogi das Cruzes SPF Brazil
    J Nanosci Nanotechnol 7:3643-52. 2007
    ..of microperoxidases (MPs) into molecular sieves with controlled pore size, such as the mesoporous silica MCM-41, represents a nanotechnology strategy to control the catalytic properties of MPs and mimic the enzymatic ..
  76. ncbi [Soil quality assessment of forest stand in different plantation esosystems]
    Yu Huang
    Huitong Experimental Station of Forest Ecology, Chinese Academy of Sciences, China
    Ying Yong Sheng Tai Xue Bao 15:2199-205. 2004
    ..Both PMS and MCM had a favorable effect on soil fertility maintenance. Soil quality assessment showed that some soil functions, e.g...
  77. doi Novel antibody screening cells, MUT+Mur kodecytes, created by attaching peptides onto red blood cells
    Damien Heathcote
    CSL Limited, Melbourne, Australia
    Transfusion 50:635-41. 2010
    ....
  78. doi Morphology, proliferation, alignment,  and new collagen synthesis of mesenchymal stem cells on a microgrooved collagen membrane
    Lili Li
    a National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, P R China
    J Biomater Sci Polym Ed 27:581-98. 2016
    ..In this study, we examined the effects of microgrooved collagen membrane (MCM) on mesenchymal stem cell (MSC) behavior...
  79. pmc A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex
    Rachael Di Santo
    Centre for Gene Regulation and Expression, College of Life Sciences, MSI WTB complex, University of Dundee, Dundee, DD1 5EH, Scotland, UK
    Mol Microbiol 92:1227-42. 2014
    ..Elongator promotes addition of mcm(5) and ncm(5) modifications to uridine in the tRNA anticodon 'wobble' position in both yeast and higher eukaryotes...
  80. ncbi Promoter methylation of tumor-related genes in gastric carcinogenesis
    Chenghai Zhao
    Department of Pathophysiology, China Medical University, Shenyang, China
    Histol Histopathol 27:1271-82. 2012
    ..number of genes with promoter methylation have been observed in gastric cancer (GC), among which p16INK4A (p16), Mut L homologue 1 (MLH1), Epithelial-cadherin (E-cadherin), Runt-related transcription factor 3 (RUNX3), adenomatous ..
  81. doi Sustained release of heparin on enlarged-pore and functionalized MCM-41
    Mi Mi Wan
    Key Laboratory of Mesoscopic Chemistry of MOE, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
    ACS Appl Mater Interfaces 4:4113-22. 2012
    Mesoporous silica MCM-41 and SBA-15 were chosen to study the adsorption and release of bulky biomolecule heparin, in order to develop new heparin controlled delivery system and expand the application of mesoporous materials in life ..
  82. pmc In vivo targeting of HER2-positive tumor using 2-helix affibody molecules
    Gang Ren
    Molecular Imaging Program at Stanford MIPS, Department of Radiology and Bio X Program, Stanford University, California, Stanford, CA 94305 5344, USA
    Amino Acids 43:405-13. 2012
    ..In our previous research, a novel cyclic 2-helix small protein, MUT-DS, was discovered as an anti-HER2 Affibody analog with high affinity through rational protein design and ..
  83. doi GINS complex protein Sld5 recruits SIK1 to activate MCM helicase during DNA replication
    Kiranmai Joshi
    Laboratory of Cell Death and Cell Survival, Centre for DNA Fingerprinting and Diagnostics CDFD, Nampally, Hyderabad 500001, India
    Cell Signal 28:1852-1862. 2016
    In eukaryotes, proper loading and activation of MCM helicase at chromosomal origins plays a central role in DNA replication...
  84. pmc Hypoxia. Cross talk between oxygen sensing and the cell cycle machinery
    Gregg L Semenza
    Vascular Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Physiol Cell Physiol 301:C550-2. 2011
    ..Components of the hexameric MCM helicase, which binds to replication origins before the onset of DNA synthesis, are present in large excess in ..
  85. pmc Genome-wide characterization of miR-34a induced changes in protein and mRNA expression by a combined pulsed SILAC and microarray analysis
    Markus Kaller
    Experimental and Molecular Pathology, Institute of Pathology, Ludwig Maximilians University Munich, Thalkirchner Strasse 36, D 80337 Munich, Germany
    Mol Cell Proteomics 10:M111.010462. 2011
    ..Proteins involved in DNA replication, e.g. the MCM proteins, and cell proliferation, were over-represented among indirectly down-regulated proteins lacking a miR-34a ..
  86. doi MISS-Prot: web server for self/non-self discrimination of protein residue networks in parasites; theory and experiments in Fasciola peptides and Anisakis allergens
    Humberto Gonzalez-Diaz
    Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain
    Mol Biosyst 7:1938-55. 2011
    ..The model combines protein residue networks, Markov Chain Models (MCM) and Artificial Neural Networks (ANN)...
  87. pmc Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding
    Aaron S Brewster
    Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089, USA
    BMC Mol Biol 11:62. 2010
    The mini-chromosome maintenance protein (MCM) complex is an essential replicative helicase for DNA replication in Archaea and Eukaryotes...
  88. pmc Anterior versus posterior surgery for multilevel cervical myelopathy, which one is better? A systematic review
    Tao Liu
    Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, People s Republic of China
    Eur Spine J 20:224-35. 2011
    ..clinical outcomes and complications of anterior surgery with posterior surgery for multilevel cervical myelopathy (MCM)...
  89. doi Baff serum level predicts time to first treatment in early chronic lymphocytic leukemia
    Stefano Molica
    Medical Oncology Unit, Hematology Oncology Department, Azienda Ospedaliera Pugliese Ciaccio, Catanzaro, Italy
    Eur J Haematol 85:314-20. 2010
    ..When three groups were considered: (1) low-risk (n=93), patients with concordant IgVH(mut) and higher soluble BAFF; (2) intermediate-risk (n=50), patients with IgVH(mut) and low BAFF levels or IgVH(unmut) ..
  90. pmc Peroxisome proliferator-activated receptor γ coactivator 1β (PGC-1β) protein attenuates vascular lesion formation by inhibition of chromatin loading of minichromosome maintenance complex in smooth muscle cells
    Yanhong Guo
    Cardiovascular Center, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 288:4625-36. 2013
    ..PGC-1β decreased the expression of minichromosome maintenance 4 (MCM4), which leads to a decreased loading of the MCM complex onto chromatin at the replication origins and decreased cyclin D1 levels, whereas PGC-1β loss of function ..
  91. doi Thermodynamic and FTIR studies of supercooled water confined to exterior and interior of mesoporous MCM-41
    Shigeharu Kittaka
    Department of Chemistry, Faculty of Science, Okayama University of Science, 1 1 Ridaicho, Okayama 700 0005, Japan
    Phys Chem Chem Phys 11:8538-43. 2009
    The thermal properties of water confined to both exterior and interior of cylindrical mesoporous MCM-41 (pore diameter d = 1.8-3.6 nm) were analysed by differential scanning calorimetry and FTIR spectroscopy...
  92. pmc Dual and specific inhibition of NAMPT and PAK4 by KPT-9274 decreases kidney cancer growth
    Omran Abu Aboud
    Division of Nephrology, Dept of Internal Medicine, University of California, Davis
    Mol Cancer Ther . 2016
    ..When KPT-9274 was administered in vivo to a 786-O (VHL-mut) human RCC xenograft model, there was dose-dependent inhibition of tumor growth with no apparent toxicity; KPT-9274 ..
  93. pmc Mutation Profile of the MUT Gene in Chinese Methylmalonic Aciduria Patients
    Mei Ying Liu
    Institute of Genetics, National Yang Ming University, Taipei, Taiwan, Republic of China
    JIMD Rep 6:55-64. 2012
    ..methylmalonic aciduria (MMA, MIM 251000) is caused by a deficiency of mitochondrial methylmalonyl-CoA mutase (MCM, E.C. 5.4.99.2) activity, which results from defects in the MUT gene...
  94. pmc P53 abnormalities and outcomes in colorectal cancer: a systematic review
    A J Munro
    Department of Surgery and Molecular Oncology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Br J Cancer 92:434-44. 2005
    ..IHC), indicating abnormal accumulation of p53, and sequence analysis, indicating presence of p53 mutations (mut). We identified 168 reports, with 241 comparisons of relevant end points and survival data on 18 766 patients...
  95. ncbi Site-specific loading of an MCM protein complex in a DNA replication initiation zone upstream of the c-MYC gene in the HeLa cell cycle
    Yayoi Kinoshita
    Department of Pathology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 279:35879-89. 2004
    The MCM proteins participate in an orderly association, beginning with the origin recognition complex, that culminates in the initiation of chromosomal DNA replication...
  96. ncbi The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3
    Yoshinori Takei
    Medical Research Council MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
    J Biol Chem 277:43121-5. 2002
    Minichromosome maintenance (MCM) proteins are essential components of pre-replication complexes, which limit DNA replication to once per cell cycle...
  97. ncbi Cell cycle control of replication initiation in eukaryotes
    S Chevalier
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK
    Curr Opin Cell Biol 8:815-21. 1996
    ..The regulation of the binding of MCM/P1 proteins to chromatin plays a key role in the replication licensing system which prevents re-replication in a ..
  98. ncbi Cholinergic and glutamatergic spontaneous and evoked excitatory postsynaptic currents in optic lobe neurons of cuttlefish, Sepia officinalis
    Abdesslam Chrachri
    Department of Biological Sciences, University of Plymouth, Portland Square, Drake Circus, Plymouth PL4 8AA, UK
    Brain Res 1020:178-87. 2004
    ..of the glutamate antagonist kynurenic acid (KYNA) and the nicotinic antagonist mecamylamine hydrochloride (MCM) resulted in complete blockade of these excitatory inputs...
  99. ncbi The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins
    S L Forsburg
    Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92186
    J Cell Sci 107:2779-88. 1994
    ..In addition, we show by classical and molecular genetics that cdc19+ is allelic to the nda1+ locus. We conclude that cdc19p plays a potentially conserved role in S phase...
  100. pmc Minichromosome maintenance (Mcm) proteins, cyclin B1 and D1, phosphohistone H3 and in situ DNA replication for functional analysis of vulval intraepithelial neoplasia
    E J Davidson
    Immunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
    Br J Cancer 88:257-62. 2003
    ..B1 (expressed in G2/early M), phosphorylated histone H3 (expressed during mitosis) and minichromosome maintenance (Mcm) proteins 2 and 5 (expressed during the cell cycle, but not in differentiated or quiescent cells)...
  101. pmc Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex
    Max E Douglas
    From The Francis Crick Institute, Clare Hall Laboratory, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 291:5879-88. 2016
    ..for the initiation of eukaryotic DNA replication and contributes in some unknown way to the activation of the Cdc45-MCM-GINS (CMG) helicase...

Research Grants63

  1. Understanding the Biological Function of MCM 10
    Anja Katrin Bielinsky; Fiscal Year: 2013
    ..Lessons learnt from simple organisms will be applied to the human system to understand the molecular pathways of cancer. ..
  2. Genetic Analysis of MSM Protein Function in S. Pombe
    Susan L Forsburg; Fiscal Year: 2012
    ..Dysregulation of the genes encoding the conserved MCM helicase occurs in cancer cells, and mcm mutations are also linked to cancer development in mice...
  3. Regulation of RPA Activity in DNA Repair
    James A Borowiec; Fiscal Year: 2012
    ..RPA selectively associates with the replication fork, by characterization of its interaction with the MCM complex...
  4. The molecular basis of RECQ4-associated genetic disorders and cancer predispositi
    Yilun Liu; Fiscal Year: 2013
    ..Our studies allow us to conclude that RECQ4 is an integral component of the MCM replicative helicase complex participating in DNA replication in human cells...
  5. GENETIC AND MOLECULAR ANALYSIS OF YEAST DNA REPLICATION
    Robert A Sclafani; Fiscal Year: 2012
    ..Three specific aims are proposed. In aim #1, we will elucidate the function and regulation of the MCM DNA helicase by investigating both the N- terminal and C- terminal [unreadable] (Beta)-fingers and the N-terminal ..
  6. Linking DNA Replication Origin Licensing with Cell Cycle Progression
    Kate E Coleman; Fiscal Year: 2013
    ..replication in S phase by the chromatin-loading of a DNA helicase known as the Mini-Chromosome Maintenance Complex (MCM) during G1 phase of the cell cycle...
  7. Regulation of MCM helicase loading and activation in the model system Saccharomyc
    Rebecca Ferguson; Fiscal Year: 2013
    ..The long-term objective of this proposal is to understand the regulation of MCM helicase function for eukaryotic chromosomal DNA replication using the model system Saccharomyces cerevisiae...
  8. The Role of the MCM2-7 Complex in the Replication Fork Processivity
    Anthony Schwacha; Fiscal Year: 2013
    ..As one of the few factors essential in both the initiation and elongation phases of DNA replication, defects in MCM activity can compromise the precision of DNA replication in multiple ways...
  9. Functional analysis of Chk2 in DNA damage response
    Jun Hyun Kim; Fiscal Year: 2013
    ..large-scale phosphoproteomic screening has resulted in the finding of new Chk2 targets, minichromosome maintenance (MCM) that might be associated with Chk2 functions in S-phase...
  10. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..structural feature in the ORC-Cdc6p complex that is similar in size to the presumptive replicative hexameric MCM helicase...
  11. Replication Initiation in Bacteria and Eukaryotes
    David Jeruzalmi; Fiscal Year: 2012
    ..Aim #3 exploits our recent identification of a unique bacterial MCM complex, which is more tractable, but should still provide a faithful model for its eukaryotic counterparts...
  12. The Regulation of DNA Replication Kinetics
    NICHOLAS R RHIND; Fiscal Year: 2013
    ..We propose that the probability of origin firing is regulated by the number of MCM complexes - the replicative helicase which establishes an origin as a site of replication initiation - loaded ..
  13. Chromatin Remodeling by Cdt1: Role in DNA Replication and Tumorigenesis
    Mark G Alexandrow; Fiscal Year: 2013
    ..Cdt1 stimulates the loading of Mini- Chromosome Maintenance (MCM) proteins at preRCs, and Geminin blocks this effect, but the molecular mechanisms behind this are unknown...
  14. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack D Griffith; Fiscal Year: 2012
    ..attached to magnetic particles will be incubated with yeast cell extracts to load the pre-RC complex including Mcm and Cdc6 factors. Their structure will be probed by Western analysis and EM using nanoscale 'biopointers'...
  15. Role of Histone H2B Ubiquitylation in DNA Replication
    KELLY MIGUEL TRUJILLO; Fiscal Year: 2013
    ..I have discovered that the MCM helicase falls off the template in htb-K123R cells that cannot be ubiquitylated at the G1-S phase transition...
  16. Structural Studies of MCM Complex
    Xiaojiang S Chen; Fiscal Year: 2012
    Project Summary: Structural studies of MCM complex Minichromosome maintenance protein (MCM) complex functions as a helicase that unwinds DNA to provide template for DNA replication and plays a critical role in regulating cell growth...
  17. Molecular mechanisms of DNA replication
    ERIC ENEMARK; Fiscal Year: 2013
    ..In human and other eukaryotic cells, the helicase engine is the hexameric MCM complex. The mechanisms that MCMs use to operate upon DNA are poorly understood at the molecular level...
  18. Structural Basis of Large T Helicase Function in SV40 DNA Replication
    Xiaojiang S Chen; Fiscal Year: 2013
    ..the helicase and cellular initiator proteins that consist of multiple initiator factors (such as Orc, Ctd1, Cdc6, MCM in eukaryotes, or DnaA/DnaC/DnaB in prokaryotes) to initiate DNA replication, i.e...
  19. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 2002
    ..The MCM proteins are a family of six related proteins that play a central role in the replication process in the yeast S...
  20. Role of Ddk Kinase in Regulation of DNA Replication
    Wei Jiang; Fiscal Year: 2006
    ..We will examine whether HsCdc7/HsDbf4 phosphorylation of MCM2 affects the formation or conformation of MCM heteromeric complexes and regulates the helicase activity of MCM complex(es)...
  21. Role of GLIS2 in the Development of Cystic Kidney Disease
    Massimo Attanasio; Fiscal Year: 2013
    ..the effect of the inhibition of TLR-2/NF-?B signaling on the inflammatory infiltration and fibrosis in Glis2mut/mut mice kidneys by generating Tlr2-/-;Glis2mut/mut and Myd88flox/flox;Glis2mut/mut;KspCre double mutants and ..
  22. FANCD2 interaction with mismatch repair proteins and MCM2-7
    Gary M Kupfer; Fiscal Year: 2013
    ..Our data indicate that binding to a subset of the MCM subunits results in inhibition of replication complex assembly...
  23. Nanotheranostics for Early Colorectal Cancer Detection and Treatment
    Jeffrey S Souris; Fiscal Year: 2013
    ..invasive M-T staging of polyp/neoplastic disease in situ, we will synthesize and tri-functionalize biocompatible MCM-41 mesoporous silica nanoparticles (MSNs) for the targeted, endoscopically-traceable, delivery of conventional ..
  24. INBORN ERRORS--MOLECULAR ANALYSIS IN CULTURED CELLS
    Leon Rosenberg; Fiscal Year: 1990
    ..to cystathionine beta-synthase (CS) deficiency; methylmalonic acidemia, due to methylmalonyl CoA mutase apoenzyme (MUT) deficiency; hyperammonemia, due to ornithine transcarbamylase (OTC) deficiency; and propionic acidemia, due to ..
  25. Regulation of V(D)J recombination by Rag2 C terminus
    Patricia Cortes; Fiscal Year: 2013
    ..will address these issues while extending our findings that showed interaction of Rag2 with core histones and the MCM complex...
  26. VEGF and Skeletal Muscle Adaptation during Chronic Overload
    KIMBERLY ANN HUEY; Fiscal Year: 2011
    ..or mice overexpressing wild type Hsp27 (human form of Hsp25) (hspTG) or a non-phosphorylatable Hsp27 mutant (mut-hspTG), we will test the hypothesis that (p)Hsp25 is a critical mediator of the angiogenic, myogenic and/or anti-..
  27. Antisense oligonucleotide treatment for myotonic dystrophy
    Charles A Thornton; Fiscal Year: 2013
    ..end, we are proposing to develop RNase H-active antisense oligonucleotides (ASOs) targeting the mutant human DMPK {mut-hDMPK) mRNA...
  28. Resolution of Epithelial Cell Hyperplasia
    Yohannes Tesfaigzi; Fiscal Year: 2013
    ..Epithelial cell hyperplasia in response to an inflammatory response is accompanied by mucous cell metaplasia (MCM), the appearance of mucous cells in airways that are normally devoid of these cells...
  29. Mechanisms of CDX2 regulation of the IGF axis
    Duyen Dang; Fiscal Year: 2013
    ..Conversely, in colon cancers with MUT-p53, CDX2 has the opposite effect...
  30. KL4 Surfactant to Mitigate Radiation-Induced Lung Injury
    Robert Segal; Fiscal Year: 2013
    ..is evaluating its proprietary peptide-based synthetic KL4 surfactant (lucinactant) as a broad-spectrum, multi-use MCM against chemical, biological, radiological and nuclear threat agents targeting the lung...
  31. Error Correction in DNA Synthesis: A Biochemical Study
    MYRON GOODMAN; Fiscal Year: 2013
    ..to transfer a molecule of RecA7ATP from its 3'- end to convert inactive pol V into mutagenically active pol V Mut. The properties of pol V Mut (UmuD'2C- RecA7ATP) are regulated through a biochemical cycle of polymerase ..
  32. Regulation of mucous cell metaplasia in asthma
    Yohannes Tesfaigzi; Fiscal Year: 2012
    ..This phenotype is called mucous cell metaplasia (MCM)...
  33. MOLECULAR GENETIC STUDIES ON METHYLMALONYL COA MUTASE
    Fred Ledley; Fiscal Year: 1992
    ..clone for methylmalonyl CoA mutase from human liver and demonstrated that this clone can be used to transduce MCM activity into cultured cells by genetic transfer...
  34. Defining the importance of CD8+ T Cell Breadth in SIV/HIV protective immunity
    DAVID H O'CONNOR; Fiscal Year: 2013
    ..We have discovered that Mauritian cynomolgus macaques (MCM) infected with simian immunodeficiency virus (SIV), a virus that causes the same disease as HIV causes in humans, ..
  35. The Role of Osteoactivin in Osteoblast Development and Function
    Fayez F Safadi; Fiscal Year: 2013
    ..During the previous funding period and since the last submission, we established colonies of OA KO and OA mut. mice, and also generated transgenic (Tg) mice that over-express OA in bone. Preliminary data from OA KO, OA mut...
  36. GABA(A) Receptor Assembly/Trafficking/Function and Epilepsy Missense Mutations
    ROBERT LOUDEN MACDONALD; Fiscal Year: 2013
    ..to a dominant negative effect of the mutant subunit, 2) the mutation alters cortical and thalamic inhibition, 3) mut [unreadable]3 (P11S) subunits are reduced in mouse brain, and 4) the mutation causes altered transcriptional ..
  37. Optimization of SWCNT/siRNA complex formulation for tumor accumulation
    Lynn Kirkpatrick; Fiscal Year: 2012
    ..Mutant KRAS (mut-KRAS) is the prototypical undruggable cancer target...
  38. BIOCHEMICAL BASIS OF SOS-INDUCED MUTAGENESIS
    MYRON GOODMAN; Fiscal Year: 2013
    ..is to transfer a molecule of RecA-ATP from its 32-end to convert inactive Pol V into mutagenically active Pol V Mut (UmuD22C-RecA-ATP)...
  39. Abstinence Reinforcement Therapy (ART) for Rural Veteran Smokers
    Patrick S Calhoun; Fiscal Year: 2013
    ..TC), a tele-medicine clinic for access to nicotine replacement (NRT), and mobile contingency management (mCM) or a control condition (i.e...
  40. Optimizing Genetic Testing for Deafness for Clinical Diagnostics
    Richard J H Smith; Fiscal Year: 2013
    ..e. low frequency hearing loss as seen with WFS1) (Hilgert et al Mut Res 2009)...
  41. MDEpiNet: MCM (Medical Countermeasures)
    Art Sedrakyan; Fiscal Year: 2012
    Summary This application proposes creation of a major innovative initiative/program on medical countermeasures (MCM) within the Medical Device Epidemiology Network (MDEpiNet)...
  42. Meiotic recombination in Drosophila
    JEFF JOSEPH SEKELSKY; Fiscal Year: 2013
    ..To better understand crossover control, we will test hypotheses for functions of a set of genes that encode MCM-like proteins essential for crossover formation, using a combination of genetic, biochemical, and cytological ..
  43. Treatment of Colitis using a Novel CXCR3 Biologic Antagonist
    James W Lillard; Fiscal Year: 2013
    ..b CXCR3 interactions, JYANT Technologies has developed a novel mutant CXCR3 ligand-immunoglobulin fusion protein (mut-CXCL11-Ig) that lacks immunogenic, glycosaminoglycan (connective tissues)-binding sites but retains the ability to ..
  44. 2D Silicon Transducer Compression Plates for Breast Ultrasound
    KAI ERIK THOMENIUS; Fiscal Year: 2010
    ..the development of a novel breast imaging device based on the use of silicon micro-machined ultrasound transducer (MUT) arrays placed on both upper and lower compression plates in a manner similar to mammography...
  45. THE MDEPINET MEDICAL COUNTER MEASURES STUDY
    Frederic S Resnic; Fiscal Year: 2012
    ..Because medical countermeasures (MCM) devices require rapid assessment and approval to ensure an effective public health response in the event of a ..
  46. Stress Signaling Pathways and Resistance in Colorectal Cancer
    Scott Kopetz; Fiscal Year: 2013
    ..and resistance to targeted therapy for CRC;and 3) conduct clinical trials of inhibitors of AKT, PI3K, and mut-KRAS signaling in patients with advanced CRC using patient tumor biopsy results to identify potential biomarkers ..
  47. Inhibiting oncogenic KRAS for cancer therapy
    Garth Powis; Fiscal Year: 2013
    ..Mutant KRAS (mut-KRAS) is the predominant oncogenic form of RAS, and is present in 17-25% of all human cancers where it plays a ..
  48. MCM Helicase as a Novel Target for Pancreatic Cancer Treatment
    Mark G Alexandrow; Fiscal Year: 2012
    ..The goal of this proposal is to explore the novel possibility that co-suppression of the replicative MCM helicase complex can enhance the anti-proliferative effects of chemotherapeutic agents used in pancreatic cancer ..
  49. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..1) Molecular characterization of mutants defective in dsRNA-mediated silencing (Mut-68, Mut-70, and Mut-91)...
  50. Molecular interactions and restoration strategies of PTEN and p53 in gliomas
    Roger Abounader; Fiscal Year: 2013
    ..tumor promoting properties by enhancing the half-life and oncogenic effects of gain-of-function p53 mutants (mut-p53)...
  51. FACTORS REGULATING TRANSPOSITION IN C. ELEGANS
    John Collins; Fiscal Year: 1992
    ..activity of Tc3, Tc4, and Tc5 has been detected only in genetic backgrounds containing the mutator mutation mut-2(r459). Genetic methods will be used to determine. if the activity of these elements is indeed mut-2-specific...
  52. Targeting mutant KRAS for cancer therapy
    Lynn Kirkpatrick; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Mutant KRAS (mut-KRAS) is the prototypical undruggable cancer target...
  53. Role of ATM in Radiosensitivity of Gliomas
    Chandan Guha; Fiscal Year: 2004
    ..t.) and U-138 (p53 mut) GBM cells resulting in enhanced radiosensitivity in vitro...
  54. ENDOTOXIN INDUCED MUCOUS CELL METAPLASIA
    Yohannes Tesfaigzi; Fiscal Year: 2003
    ..that Bcl-2 expression (a gene that inhibits apoptosis or programmed cell death) plays in mucous cell metaplasia (MCM)...
  55. Defining non-classical, CMV-induced CD8 T cell responses
    Jonah B Sacha; Fiscal Year: 2013
    ..In contrast, Mauritian cynomolgus macaques (MCM), descendants from a population bottleneck event, exhibit simplified MHC genetics with only seven completely ..
  56. Canine model for assessing CNS access P-gp substrates
    KATRINA MEALEY; Fiscal Year: 2005
    The overall goal of the proposed research is to validate a P-glycoprotein (P-gp) deficient canine model [MDR1(mut/mut)] for delineating the role of P-gp in restricting access of therapeutic agents to the central nervous system (CNS)...
  57. PROTEIN PHOSPHATASE 2A AND TUMOR ANTIGENS IN CELL GROWTH
    Gernot Walter; Fiscal Year: 2004
    ..The main focus will be on identifying the substrate(s) of PP2A. Potential candidates are ORC, cdc6, MCM, cdc45, RPA, and DNA polymerase alpha, known to be involved in initiation...
  58. REGULATION OF MISMATCH REPAIR IN A MODEL E COLI SYSTEM
    ALICIA DOMBROSKI; Fiscal Year: 2001
    DESCRIPTION: The long-term goal of this project is to understand the regulatio of the mut S, mutL, and mut H DNA repair genes and the possible implications o this regulation to DNA repair, spontaneous and induced mutagenesis, and ..
  59. FISSION YEAST DNA REPLICATION ORGINS
    Joel Huberman; Fiscal Year: 2001
    ..ORC binding appears necessary for the subsequent binding of Cdc6p, the Mcm family of proteins, and perhaps other proteins at the beginning of G1 phase...
  60. EFFECTS OF 03/ENDOTOXIN COEXPOSURE ON NASAL EPITHELIUM
    James Wagner; Fiscal Year: 2000
    ..Ozone exposure causes inflammation that is followed by mucous cell metaplasia (MCM) which is the appearance of mucous secreting cells in regions of nasal epithelium normally devoid of secretory ..
  61. MOOD AND MEMORY
    JOHN KIHLSTROM; Fiscal Year: 1990
    ..autobiographical memory designs to explore the phenomena of mood- dependent memory (MDM) and mood-congruent memory (MCM) and resource allOcation effects...
  62. Development of Medical Countermeasures to Mitigate or Treat GI-ARS
    THOMAS RIVENBURG; Fiscal Year: 2013
    The contracts awarded under this initiative will advance the development of candidate medical countermeasures (MCM) that can mitigate or treat gastrointestinal (GI) syndrome of the acute radiation syndrome (GI-ARS) and increase survival...