MTAP

Summary

Gene Symbol: MTAP
Description: methylthioadenosine phosphorylase
Alias: BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MeSAdo phosphorylase, epididymis luminal protein 249
Species: human

Top Publications

  1. pmc Genomic cloning of methylthioadenosine phosphorylase: a purine metabolic enzyme deficient in multiple different cancers
    T Nobori
    The Sam and Rose Stein Institute for Research on Aging, and Department of Medicine, University of California at San Diego, La Jolla, 92093 0663, USA
    Proc Natl Acad Sci U S A 93:6203-8. 1996
  2. ncbi Methylthioadenosine phosphorylase, a gene frequently codeleted with p16(cdkN2a/ARF), acts as a tumor suppressor in a breast cancer cell line
    Scott A Christopher
    Fox Chase Cancer Center, Division of Population Science, Philadelphia, Pennsylvania 19111, USA
    Cancer Res 62:6639-44. 2002
  3. pmc Characterization of methylthioadenosin phosphorylase (MTAP) expression in malignant melanoma
    Iris Behrmann
    Institute of Biochemistry, RWTH Aachen, Aachen, Germany
    Am J Pathol 163:683-90. 2003
  4. ncbi Strong expression of methylthioadenosine phosphorylase (MTAP) in human colon carcinoma cells is regulated by TCF1/[beta]-catenin
    Frauke Bataille
    Institute of Pathology, University of Regensburg Medical School, Regensburg, Germany
    Lab Invest 85:124-36. 2005
  5. ncbi Methylthioadenosine phosphorylase gene expression is impaired in human liver cirrhosis and hepatocarcinoma
    Carmen Berasain
    Division of Hepatology and Gene Therapy, CIMA, Facultad de Medicina, Universidad de Navarra 31008 Pamplona, Spain
    Biochim Biophys Acta 1690:276-84. 2004
  6. ncbi Promoter-hypermethylation is causing functional relevant downregulation of methylthioadenosine phosphorylase (MTAP) expression in hepatocellular carcinoma
    Claus Hellerbrand
    Department of Internal Medicine I and Institute of Pathology, University of Regensburg, D 93042 Regensburg, Germany
    Carcinogenesis 27:64-72. 2006
  7. ncbi Lack of methylthioadenosine phosphorylase expression in mantle cell lymphoma is associated with shorter survival: implications for a potential targeted therapy
    Silvia Marcé
    Pathology Department, Hematopathology Unit, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer, University of Barcelona, Barcelona, Spain
    Clin Cancer Res 12:3754-61. 2006
  8. doi Redox regulation of methylthioadenosine phosphorylase in liver cells: molecular mechanism and functional implications
    Joaquín Fernández-Irigoyen
    Division of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, 31008 Pamplona, Spain
    Biochem J 411:457-65. 2008
  9. pmc Genome-wide association study identifies three loci associated with melanoma risk
    D Timothy Bishop
    Section of Epidemiology and Biostatistics, Leeds Institute of Molecular Medicine, Cancer Research UK Clinical Centre at Leeds, St James s University Hospital, Leeds, UK
    Nat Genet 41:920-5. 2009
  10. pmc Genome-wide association study identifies variants at 9p21 and 22q13 associated with development of cutaneous nevi
    Mario Falchi
    Department of Twin Research and Genetic Epidemiology, Kings College London, St Thomas Hospital Campus, London, UK
    Nat Genet 41:915-9. 2009

Research Grants

  1. Vern L Schramm; Fiscal Year: 2016
  2. Purine salvage as a chemotherapeutic target in malaria
    Kami Kim; Fiscal Year: 2002
  3. The Role of Methylthioadenosine Phosphorylase Loss in Tumorigenesis
    Warren D Kruger; Fiscal Year: 2013
  4. Colon Polyp Surveillance with DNA Analysis of Stool
    Philip Schoenfeld; Fiscal Year: 2003
  5. Reagents for the Design of Targeted Multifunctional Nanomaterials
    Lee Josephson; Fiscal Year: 2012
  6. CHROMOSOME 9P ABERRATIONS IN GLIOMA
    Dennis Carson; Fiscal Year: 1999
  7. CHARACTERIZATION OF A TUMOR SUPPRESSOR GENE ON 9P
    Olufunmilayo Olopade; Fiscal Year: 1999
  8. Mechanism of Action of Folate Antagonist
    Joseph Bertino; Fiscal Year: 2008
  9. PERTUSSIS, DIPHTHERIA, CHOLERA TOXINS-INHIBITOR DESIGN
    Vern Schramm; Fiscal Year: 2003
  10. TRANSITION STATE ANALYSIS OF ENZYMATIC REACTIONS
    Vern Schramm; Fiscal Year: 2007

Detail Information

Publications223 found, 100 shown here

  1. pmc Genomic cloning of methylthioadenosine phosphorylase: a purine metabolic enzyme deficient in multiple different cancers
    T Nobori
    The Sam and Rose Stein Institute for Research on Aging, and Department of Medicine, University of California at San Diego, La Jolla, 92093 0663, USA
    Proc Natl Acad Sci U S A 93:6203-8. 1996
    ..2.28; MTAP) plays a role in purine and polyamine metabolism and in the regulation of transmethylation reactions...
  2. ncbi Methylthioadenosine phosphorylase, a gene frequently codeleted with p16(cdkN2a/ARF), acts as a tumor suppressor in a breast cancer cell line
    Scott A Christopher
    Fox Chase Cancer Center, Division of Population Science, Philadelphia, Pennsylvania 19111, USA
    Cancer Res 62:6639-44. 2002
    The human methylthioadenosine phosphorylase (MTAP) gene is located on 9p21 and is frequently homozygously deleted, along with p16(cdkN2a/ARF), in a wide variety of human tumors and human tumor-derived cell lines...
  3. pmc Characterization of methylthioadenosin phosphorylase (MTAP) expression in malignant melanoma
    Iris Behrmann
    Institute of Biochemistry, RWTH Aachen, Aachen, Germany
    Am J Pathol 163:683-90. 2003
    ..In the same chromosomal region the methylthioadenosine phosphorylase (MTAP) gene is localized and therefore may also serve as a tumor suppressor gene...
  4. ncbi Strong expression of methylthioadenosine phosphorylase (MTAP) in human colon carcinoma cells is regulated by TCF1/[beta]-catenin
    Frauke Bataille
    Institute of Pathology, University of Regensburg Medical School, Regensburg, Germany
    Lab Invest 85:124-36. 2005
    Methylthioadenosine phosphorylase (MTAP) is known as a ubiquitously expressed house keeping gene important in biochemical salvage processes. The MTAP gene is localized on the human chromosomal region 9p21, a region often deleted in cancer...
  5. ncbi Methylthioadenosine phosphorylase gene expression is impaired in human liver cirrhosis and hepatocarcinoma
    Carmen Berasain
    Division of Hepatology and Gene Therapy, CIMA, Facultad de Medicina, Universidad de Navarra 31008 Pamplona, Spain
    Biochim Biophys Acta 1690:276-84. 2004
    Methylthioadenosine phosphorylase (MTAP) is a key enzyme in the methionine and adenine salvage pathways...
  6. ncbi Promoter-hypermethylation is causing functional relevant downregulation of methylthioadenosine phosphorylase (MTAP) expression in hepatocellular carcinoma
    Claus Hellerbrand
    Department of Internal Medicine I and Institute of Pathology, University of Regensburg, D 93042 Regensburg, Germany
    Carcinogenesis 27:64-72. 2006
    The methylthioadenosine phosphorylase (MTAP) gene is localized in the chromosomal region 9p21. Here, frequently homozygous deletions occur in several kinds of cancer associated with the loss of tumour suppressor genes as p16 and p15...
  7. ncbi Lack of methylthioadenosine phosphorylase expression in mantle cell lymphoma is associated with shorter survival: implications for a potential targeted therapy
    Silvia Marcé
    Pathology Department, Hematopathology Unit, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer, University of Barcelona, Barcelona, Spain
    Clin Cancer Res 12:3754-61. 2006
    To determine the methylthioadenosine phosphorylase (MTAP) gene alterations in mantle cell lymphoma (MCL) and to investigate whether the targeted inactivation of the alternative de novo AMP synthesis pathway may be a useful therapeutic ..
  8. doi Redox regulation of methylthioadenosine phosphorylase in liver cells: molecular mechanism and functional implications
    Joaquín Fernández-Irigoyen
    Division of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, 31008 Pamplona, Spain
    Biochem J 411:457-65. 2008
    b>MTAP (5'-methylthioadenosine phosphorylase) catalyses the reversible phosphorolytic cleavage of methylthioadenosine leading to the production of methylthioribose-1-phosphate and adenine...
  9. pmc Genome-wide association study identifies three loci associated with melanoma risk
    D Timothy Bishop
    Section of Epidemiology and Biostatistics, Leeds Institute of Molecular Medicine, Cancer Research UK Clinical Centre at Leeds, St James s University Hospital, Leeds, UK
    Nat Genet 41:920-5. 2009
    ..54 x 10(-27) for rs258322), 11q14-q21 encompassing TYR (P = 2.41 x 10(-14) for rs1393350) and 9p21 adjacent to MTAP and flanking CDKN2A (P = 4.03 x 10(-7) for rs7023329)...
  10. pmc Genome-wide association study identifies variants at 9p21 and 22q13 associated with development of cutaneous nevi
    Mario Falchi
    Department of Twin Research and Genetic Epidemiology, Kings College London, St Thomas Hospital Campus, London, UK
    Nat Genet 41:915-9. 2009
    ..We identified strongly associated variants in MTAP, a gene adjacent to the familial melanoma susceptibility locus CDKN2A on 9p21 (rs4636294, combined P = 3...
  11. doi Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors
    Hsuan Ying Huang
    Departments of Pathology, Surgery, and Radiation Oncology, and Divisions of Oncology and Gastroenterology, Department of Internal Medicine, Chang Gung Memorial Hospital Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
    Clin Cancer Res 15:6963-72. 2009
    ..We did an integrative study of MTAP gene at 9p21 to analyze its implication in GISTs.
  12. pmc Melanocytic nevi, nevus genes, and melanoma risk in a large case-control study in the United Kingdom
    Julia A Newton-Bishop
    Section of Epidemiology and Biostatistics, Leeds Institute of Molecular Medicine, St James s Hospital, Beckett Street, Leeds, United Kingdom
    Cancer Epidemiol Biomarkers Prev 19:2043-54. 2010
    ..Increased number of melanocytic nevi is a potent melanoma risk factor. We have carried out a large population-based case-control study to explore the environmental and genetic determinants of nevi and the relationship with melanoma risk...
  13. pmc Growth and metastases of human lung cancer are inhibited in mouse xenografts by a transition state analogue of 5'-methylthioadenosine phosphorylase
    Indranil Basu
    Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA
    J Biol Chem 286:4902-11. 2011
    The S-adenosylmethionine (AdoMet) salvage enzyme 5'-methylthioadenosine phosphorylase (MTAP) has been implicated as both a cancer target and a tumor suppressor. We tested these hypotheses in mouse xenografts of human lung cancers...
  14. pmc Down-regulation of methylthioadenosine phosphorylase (MTAP) induces progression of hepatocellular carcinoma via accumulation of 5'-deoxy-5'-methylthioadenosine (MTA)
    Georgi Kirovski
    Department of Internal Medicine I, University Hospital Regensburg, Regensburg, Germany
    Am J Pathol 178:1145-52. 2011
    Recently, we have shown that down-regulation of methylthioadenosine phosphorylase (MTAP) in hepatocellular carcinoma (HCC) cells enhances the invasive potential and the resistance against cytokines...
  15. pmc Evaluation of nucleoside hydrolase inhibitors for treatment of African trypanosomiasis
    Maya Berg
    Laboratory of Medicinal Chemistry, University of Antwerp, Antwerp, Belgium
    Antimicrob Agents Chemother 54:1900-8. 2010
    ..b. brucei (IAG-NH, IG-NH, and methylthioadenosine phosphorylase [MTAP]) were investigated using RNA interference...
  16. ncbi Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds
    G Cacciapuoti
    Institute of Biochemistry of Macromolecules, Faculty of Medicine, Second University of Naples, Italy
    J Biol Chem 269:24762-9. 1994
    ..solfataricus the same enzyme is responsible for the catabolism of MTA and of these purine nucleosides...
  17. ncbi Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus
    Giovanna Cacciapuoti
    Dipartimento di Biochimica e Biofisica F Cedrangolo, Seconda Universita di Napoli, Napoli, Italy
    FEBS J 274:2482-95. 2007
    ..a homohexamer of 180 kDa which shows a much higher similarity with 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) than with purine nucleoside phosphorylase (PNP) family members...
  18. ncbi Three-dimensional structure of a hyperthermophilic 5'-deoxy-5'-methylthioadenosine phosphorylase from Sulfolobus solfataricus
    T C Appleby
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 276:39232-42. 2001
    ..Examination of the three-dimensional structure of SsMTAP suggests that unlike most thermophilic enzymes, disulfide linkages play a key in role in its thermal stability...
  19. ncbi Endosperm-specific hypomethylation, and meiotic inheritance and variation of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) inter-strain hybrids
    M S Zhang
    Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024, China
    Theor Appl Genet 115:195-207. 2007
    ..parents, the developmental stability and inheritance of cytosine methylation in two tissues, leaf and endosperm, by MSAP analysis were investigated...
  20. doi Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding
    Giovanna Cacciapuoti
    Department of Biochemistry and Biophysics F Cedrangolo, Second University of Naples, Italy
    FEBS J 276:5799-805. 2009
    ..The data obtained in the present study represent the first example of how the CXC motif improves both stability and folding in hyperthermophilic proteins with disulfide bonds...
  21. ncbi Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus: substrate specificity and primary structure analysis
    Giovanna Cacciapuoti
    Dipartimento di Biochimica e Biofisica F Cedrangolo, Seconda Universita di Napoli, Via Costantinopoli 16, 80138, Naples, Italy
    Extremophiles 7:159-68. 2003
    5'-Methylthioadenosine phosphorylase (MTAP) was purified to homogeneity from the hyperthermophilic archaeon Pyrococcus furiosus. The protein is a homoexamer of 180 kDa...
  22. pmc Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability
    Hongyan Wang
    Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics and Cytology, Northeast Normal University, Changchun, PR China
    BMC Plant Biol 9:63. 2009
    ....
  23. ncbi Sympathoadrenal reactions during asphyxia in hypoglycemia and hyperglycemia of cats
    S W Kuo
    Department of Medicine, Tri Service General Hospital, National Defense Medical Center, Taipei, Taiwan
    Chin J Physiol 37:63-71. 1994
    ..5 gm/kg/hr. During hypo- or hyperglycemia, resting mean systemic arterial pressure (MSAP) and heart rate did not change significantly. Brain transection at various levels, i.e...
  24. ncbi Influence of cryopreservation on the cytosine methylation state of potato genomic NDA
    Anja Kaczmarczyk
    Leibniz Institute of Plant Genetics and Crop Plant Research IPK, Gatersleben, Germany
    Cryo Letters 31:380-91. 2010
    ..epigenetic changes, the DNA methylation status was assayed by methylation-sensitive amplified polymorphism (MSAP) analysis using restriction endonucleases MspI and HpaII. An amount of 93...
  25. ncbi The crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase II from Sulfolobus solfataricus, a thermophilic enzyme stabilized by intramolecular disulfide bonds
    Yan Zhang
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853 1301, USA
    J Mol Biol 357:252-62. 2006
    ....
  26. ncbi Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L
    Massimo Labra
    Dipartimento di Scienze dell Ambiente e del Territorio, Universita degli Studi di Milano Bicocca, Piazza della Scienza 1, 20126 Milano, Italy
    Chemosphere 54:1049-58. 2004
    ..using a monoclonal antibody against 5-methylcytosine; and (ii) methylation-sensitive amplified polymorphism (MSAP). Immunolabelling showed cytosine-hypermethylation in the Brassica napus L...
  27. ncbi A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus
    Giovanna Cacciapuoti
    Dipartimento di Biochimica e Biofisica F Cedrangolo, Seconda Universita di Napoli, Italy
    FEBS J 272:1886-99. 2005
    ....
  28. doi Comparison of cerebral glucose metabolism between multiple system atrophy Parkinsonian type and Parkinson's disease
    Tao Feng
    Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
    Neurol Res 30:377-82. 2008
    ..To investigate the difference in the regional cerebral glucose metabolism between multiple system atrophy Parkinsonian type (MSA-P) and Parkinson's disease (PD)...
  29. ncbi Methylthioadenosine phosphorylase as target for chemoselective treatment of T-cell acute lymphoblastic leukemic cells
    Thomas Efferth
    Virtual Campus Rhineland Palatinate, Mainz, Germany
    Blood Cells Mol Dis 28:47-56. 2002
    We analyzed the role of methylthioadenosine phosphorylase (MTAP) for chemoselective treatment of T-cell acute lymphoblastic leukemia (T-ALL)...
  30. doi Alterations in cytosine methylation and species-specific transcription induced by interspecific hybridization between Oryza sativa and O. officinalis
    Huajun Jin
    Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China
    Theor Appl Genet 117:1271-9. 2008
    ..In this study, we used the methylation-sensitive amplified polymorphism (MSAP) method to survey cytosine methylation alterations that occurred in F(1) hybrid and BC(1) progeny following ..
  31. pmc Extensions to the Speech Disorders Classification System (SDCS)
    Lawrence D Shriberg
    Waisman Center, University of Wisconsin Madison, Madison, WI, USA
    Clin Linguist Phon 24:795-824. 2010
    ..Part II describes the Madison Speech Assessment Protocol (MSAP), an ∼ 2-hour battery of 25 measures that includes 15 speech tests and tasks...
  32. doi Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma
    Josh Sommer
    Chordoma Foundation, Greensboro, NC, USA, and Division of Medical Oncology, Department of Medicine, Duke University Medical Center and Durham Veterans Affairs Medical Center, Durham, NC, USA
    J Pathol 220:608-17. 2010
    ..Another gene in this region, methylthioadenosine phosphorylase (MTAP), is an essential enzyme of the purine salvage pathway and has therapeutic relevance because MTAP-deficient cells ..
  33. ncbi Expression of methylthioadenosine phosphorylase cDNA in p16-, MTAP- malignant cells: restoration of methylthioadenosine phosphorylase-dependent salvage pathways and alterations of sensitivity to inhibitors of purine de novo synthesis
    Z H Chen
    Cancer Center, University of Massachusetts Medical Center, Worcester, Massachusetts 01655, USA
    Mol Pharmacol 52:903-11. 1997
    5'-Deoxy-5'-methylthioadenosine phosphorylase (MTAP) is involved in the salvage of adenine and methylthio moieties of 5'-deoxy-5'-methylthioadenosine, a byproduct of polyamine synthesis, to adenine nucleotides and methionine, respectively...
  34. doi The allotetraploid Arabidopsis thaliana-Arabidopsis lyrata subsp. petraea as an alternative model system for the study of polyploidy in plants
    Julien Beaulieu
    Departement de Phytologie, Universite Laval, 1243 Pavillon C E Marchand, Quebec, QC, G1K 7P4, Canada
    Mol Genet Genomics 281:421-35. 2009
    ..Changes in the degree of methylation were observed at almost 25% of the loci examined by MSAP analysis...
  35. doi Genetic and epigenetic instability of amplification-prone sequences of a novel B chromosome induced by tissue culture in Plantago lagopus L
    Gurmeet Kour
    Plant Genomics Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, India
    Plant Cell Rep 28:1857-67. 2009
    ..and epigenetic mechanisms were assessed in regenerants using molecular techniques, namely, PCR-RFLP, SSAP and MSAP. The retrotransposon-based molecular markers were applied to detect the polymorphism within transposable elements ..
  36. ncbi Nitric oxide produces different actions in different areas of the periaqueductal grey in cats
    M R Wang
    Graduate Institute of Life Science, National Defense Medical Center, Taiwan, Republic of, Taipei, China
    Neurosci Lett 309:57-61. 2001
    ..the midbrain elicited similar patterns of cardiovascular responses: increases in mean systemic arterial pressure (MSAP), heart rate (HR) and mean blood flows (F) of the common carotid and femoral arteries, accompanied with a 'hissing-..
  37. ncbi Conformational changes and stabilization induced by phosphate binding to 5'-methylthioadenosine phosphorylase from the thermophilic archaeon Sulfolobus solfataricus
    G Cacciapuoti
    Dipartimento di Biochimica e Biofisica F Cedrangolo, Seconda Universita degli Studi di Napoli, Via Costantinopoli 16, 80138, Naples, Italy
    Extremophiles 5:295-302. 2001
    ....
  38. ncbi [Analysis of genomic DNA methylation level in radish under cadmium stress by methylation-sensitive amplified polymorphism technique]
    Jin Lan Yang
    National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
    Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 33:219-26. 2007
    ..genome was analysed using the technique of methylation-sensitive amplified polymorphism (MSAP)...
  39. doi Epigenetic differentiation and relationship to adaptive genetic divergence in discrete populations of the violet Viola cazorlensis
    Carlos M Herrera
    Estacion Biologica de Donana, Consejo Superior de Investigaciones Cientificas CSIC, Isla de la Cartuja, Sevilla, Spain
    New Phytol 187:867-76. 2010
    ..the southern Spanish violet Viola cazorlensis (Violaceae) using the methylation-sensitive amplified polymorphism (MSAP) technique, a modification of the amplified fragment length polymorphism method (AFLP) based on the differential ..
  40. doi Prenatal exposure to zymosan results in hypertension in adult offspring rats
    Wenqiang Liao
    National Integrative Medicine Centre for Cardiovascular Diseases, China Japan Friendship Hospital, Beijing, China
    Clin Exp Pharmacol Physiol 35:1413-8. 2008
    ..a non-infectious and non-bacterial agent capable of inducing inflammation, on mean systolic arterial pressure (MSAP) in rat offspring at 6-66 weeks of age. 2...
  41. ncbi Tissue culture-induced locus-specific alteration in DNA methylation and its correlation with genetic variation in Codonopsis lanceolata Benth. et Hook. f
    W L Guo
    Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
    Plant Cell Rep 26:1297-307. 2007
    ..of cytosine methylation at the CCGG sites in the same set of regenerants relative to the donor plant, by the MSAP method employing a pair of isoschizomers, HpaII and MspI, which recognize the same restriction site but are ..
  42. ncbi Using specific cytotoxics with a targeted mind
    Angelo Di Leo
    Sandro Pitigliani Medical Oncology Unit, Department of Oncology, Hospital of Prato, Tuscany Cancer Institute, Prato, Italy
    Breast 16:S120-6. 2007
    ..b) Taxanes Microtubule-associated parameters (MTAP) such as the TAU protein, HER-2 gene amplification, and p-53 gene mutations, have been suggested as potential ..
  43. doi Alteration of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) pure-lines and inter-line F1 hybrids following low-dose laser irradiation
    Huan Wang
    Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
    J Photochem Photobiol B 99:150-3. 2010
    ..level and pattern of DNA methylation in the plants were analyzed by methylation-sensitive amplified polymorphism (MSAP)...
  44. pmc Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil
    Mariana Ferreira Leal
    Genetics Division, Department of Morphology, Federal University of Sao Paulo, Sao Paulo, SP, Brazil
    World J Gastroenterol 13:2568-74. 2007
    To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics.
  45. doi Construction of methylation linkage map based on MSAP and SSR markers in Sorghum bicolor (L.)
    Yonghong Duan
    Institute of Biotechnology, Shanxi University, Taiyuan, China
    IUBMB Life 61:663-9. 2009
    ..a F2 population derived from a cross of B(2)V(4) x 1383-2 based on methylation-sensitive amplified polymorphism (MSAP) and simple sequence repeats (SSR) markers...
  46. doi Genome-wide DNA methylation alterations of Alternanthera philoxeroides in natural and manipulated habitats: implications for epigenetic regulation of rapid responses to environmental fluctuation and phenotypic variation
    Lexuan Gao
    Center for Evolutionary Biology and Institute of Biodiversity Science, Fudan University, Shanghai 200433, China
    Plant Cell Environ 33:1820-7. 2010
    ..Genome-wide methylation profiling using methylation-sensitive amplified fragment length polymorphism (MSAP) revealed considerable DNA methylation polymorphisms within and between natural populations...
  47. pmc Analysis of DNA methylation in various swine tissues
    Chun Yang
    College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China
    PLoS ONE 6:e16229. 2011
    ..In this study, we used the fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) method to assess the extent and pattern of cytosine methylation in muscle, heart, liver, spleen, lung, kidney and ..
  48. pmc Cytosine methylation alteration in natural populations of Leymus chinensis induced by multiple abiotic stresses
    Yingjie Yu
    Institute of Grassland Science, Northeast Normal University, Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun, PR China
    PLoS ONE 8:e55772. 2013
    ..at the population level by using the amplified fragment length polymorphism (AFLP), methylation-sensitive amplified polymorphism (MSAP) and retrotransposon based sequence-specific amplification polymorphism (SSAP) techniques.
  49. pmc Quantitation of cellular metabolic fluxes of methionine
    Tomer Shlomi
    Dept of Computer Science, Technion Israel Institute of Technology, Haifa 32000, Israel
    Anal Chem 86:1583-91. 2014
    ..a human fibrosarcoma cell line and study how methionine salvage pathway enzyme methylthioadenosine phosphorylase (MTAP), frequently deleted in cancer, affects methionine metabolism...
  50. pmc Epigenetic variability in the genetically uniform forest tree species Pinus pinea L
    Enrique Sáez-Laguna
    Departamento de Ecología y Genética Centro de Investigación Forestal CIFOR, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA, Madrid, Spain Unidad Mixta de Genómica y Ecofisiología Forestal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Universidad Politécnica de Madrid UPM, Madrid, Spain
    PLoS ONE 9:e103145. 2014
    ..pinea were analyzed with the Methylation Sensitive Amplified Polymorphism (MSAP) technique. A high degree of cytosine methylation was detected (64.36% of all scored DNA fragments)...
  51. doi Homozygous losses detected by array comparative genomic hybridization in multiplex urothelial carcinomas of the bladder
    Tamas Beothe
    Department of Urology, Medical School, University of Pecs, Pecs, Hungary
    Cancer Genet 208:434-40. 2015
    ..Copy number losses and homozygous deletions at the chromosome 9p region affecting the CDKN2A and MTAP genes were the most frequent alterations in both groups of tumors...
  52. pmc Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells
    Frederik C Henrich
    Department of Internal Medicine 5 Hematology and Oncology, University Hospital of Erlangen, Erlangen, Germany
    Oncoimmunology 5:e1184802. 2016
    ..intratumoral MTA levels result from a lack of the MTA-catabolizing enzyme methylthioadenosine phosphorylase (MTAP) in tumor cells and are found in various tumor entities...
  53. ncbi Extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Gene cloning and amino acid sequence determination
    G Cacciapuoti
    Istituto di Biochimica delle Macromolecole, Facolta di Medicina e Chirurgia, Seconda Universita degli Studi di Napoli, Italy
    Eur J Biochem 239:632-7. 1996
    ..Evolutionary and structural implications of this similarity are discussed...
  54. pmc Diaphyseal medullary stenosis with malignant fibrous histiocytoma: a hereditary bone dysplasia/cancer syndrome maps to 9p21-22
    J A Martignetti
    Department of Human Genetics, Mount Sinai School of Medicine, Box 1498, Fifth Avenue at 100th Street, New York, NY 10029, USA
    Am J Hum Genet 64:801-7. 1999
    ....
  55. ncbi Effect of cryopreservation on apple genetic resources at morphological, chromosomal, and molecular levels
    Y J Hao
    National Key Laboratory of Crop Genetic Improvement, Wuhan 430070, People s Republic of China
    Cryobiology 43:46-53. 2001
    ..In addition, methylation-sensitive amplified polymorphism (MSAP) assay was carried out to investigate the DNA methylation status during the period of cryopreservation...
  56. ncbi Genetic and epigenetic evaluations of citrus calluses recovered from slow-growth culture
    Yu Jin Hao
    National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P R China
    J Plant Physiol 161:479-84. 2004
    ..No difference in RAPD pattern was found with the 102 primers used. However, a methylation sensitive amplified polymorphism (MSAP) assay showed DNA methylation changes in the stored samples compared with the controls.
  57. pmc Comprehensive analysis of the 9p21 region in neuroblastoma suggests a role for genes mapping to 9p21-23 in the biology of favourable stage 4 tumours
    J Mora
    Department of Molecular Pathology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Br J Cancer 91:1112-8. 2004
    ..in this region (cyclin-dependent kinase inhibitor 2A - CDKN2A/p16(INK4a), CDKN2A/p14(ARF), CDKN2B/p15(INK4b), MTAP, interferon alpha and beta cluster). LOH was detected in 16.4% of 177 NB...
  58. ncbi MSAP enhances migration of C6 glioma cells through phosphorylation of the myosin regulatory light chain
    B C Bornhauser
    Department of Neuroscience, Neurobiology, Biomedical Centre, Uppsala University, Box 587, 75123 Uppsala, Sweden
    Cell Mol Life Sci 62:1260-6. 2005
    ..Here we show that MRLC-interacting protein (MIR)-interacting saposin-like protein (MSAP) enhances cell spreading in fibroblasts and migration of rat C6 glioma cells through increases in MRLC ..
  59. ncbi Second generation transition state analogue inhibitors of human 5'-methylthioadenosine phosphorylase
    Gary B Evans
    Carbohydrate Chemistry, Industrial Research Limited, Lower Hutt, New Zealand
    J Med Chem 48:4679-89. 2005
    ..In humans MTA is processed by 5'-methylthioadenosine phosphorylase (MTAP) so that significant amounts of MTA do not accumulate...
  60. pmc Rats with steroid-induced polycystic ovaries develop hypertension and increased sympathetic nervous system activity
    Elisabet Stener-Victorin
    Cardiovascular Institute, Wallenberg Laboratory, Sahlgrenska Academy, Goteborg University, SE 413 45 Goteborg, Sweden
    Reprod Biol Endocrinol 3:44. 2005
    ..Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction, abdominal obesity, hyperandrogenism, hypertension, and insulin resistance...
  61. ncbi Array-comparative genomic hybridization to detect genomewide changes in microdissected primary and metastatic oral squamous cell carcinomas
    Chung Ji Liu
    School of Dentistry, National Yang Ming University, Taipei, Taiwan
    Mol Carcinog 45:721-31. 2006
    ..The highest frequencies of losses were detected in p44S10, TIF1, LPL, MTAP, BMI1, EGR2, and MAP2K5...
  62. doi A complete inventory of all enzymes in the eukaryotic methionine salvage pathway
    Ivan Pirkov
    Department of Chemical and Biological Engineering, Molecular Biotechnology, Chalmers University of Technology, Goteborg, Sweden
    FEBS J 275:4111-20. 2008
    ..Thus, for the first time, all enzymes of the methionine salvage pathway were identified in a eukaryote...
  63. pmc Design and synthesis of potent "sulfur-free" transition state analogue inhibitors of 5'-methylthioadenosine nucleosidase and 5'-methylthioadenosine phosphorylase
    Alistair I Longshaw
    Carbohydrate Chemistry Team, Industrial Research Limited, P O Box 31310, Lower Hutt, New Zealand
    J Med Chem 53:6730-46. 2010
    ..In humans, 5'-methylthioadenosine phosphorylase (MTAP) is involved in polyamine biosynthesis as well as in purine and SAM salvage pathways and thus has been identified ..
  64. pmc Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis
    Kajal Buckoreelall
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 1530 Third Avenue South, Birmingham, AL 35294, USA
    Tuberculosis (Edinb) 92:139-47. 2012
    5'-methylthioadenosine (MTA) is a natural purine that is metabolized by methylthioadenosine phosphorylase (MTAP, E.C 2.4.2.28) in Eukarya and Archaea but generally not in bacteria...
  65. doi msap: a tool for the statistical analysis of methylation-sensitive amplified polymorphism data
    A Pérez-Figueroa
    Departamento de Bioquimica, Genética e Inmunología, Facultad de Biologia, Universidad de Vigo, 36310, Vigo, Spain
    Mol Ecol Resour 13:522-7. 2013
    In this study msap, an R package which analyses methylation-sensitive amplified polymorphism (MSAP or MS-AFLP) data is presented...
  66. doi Mycosis fungoides and Sézary syndrome
    Blanca Espinet
    Molecular Cytogenetics Laboratory, Pathology Department, Hospital del Mar, Barcelona, Spain
    Methods Mol Biol 973:175-88. 2013
    ..In MF, three specific chromosomal regions, 9p21.3 (CDKN2A, CDKN2B, and MTAP), 8q24...
  67. doi Medial sural artery perforator free flap: legitimate use as a solution for the ipsilateral distal lower extremity defect
    Geoffrey G Hallock
    Division of Plastic Surgery, Sacred Heart and The Lehigh Valley Hospitals, Allentown, Pennsylvania, St Luke s Hospital, Bethlehem, Pennsylvania
    J Reconstr Microsurg 30:187-92. 2014
    The medial sural artery perforator (MSAP) flap captures the cutaneous territory of the medial calf, and can potentially be advantageous as a thin free flap, even in the most obese individual, for coverage of most foot and/or ankle defects ..
  68. doi Differential DNA methylation between two wing phenotypes adults of Sogatella furcifera
    Xiaosui Zhou
    State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat Sen University, Guangzhou, Guangdong, People s Republic of China
    Genesis 51:819-26. 2013
    ..of methylation changes associated with wing phenotypes by using methylation-sensitive amplification polymorphism (MSAP)...
  69. doi Methylation-sensitive amplified polymorphism (MSAP) marker to investigate drought-stress response in Montepulciano and Sangiovese grape cultivars
    Emidio Albertini
    Department of Applied Biology, University of Perugia, Perugia, Italy
    Methods Mol Biol 1112:151-64. 2014
    Methylation-sensitive amplified polymorphism (MSAP) is a technique developed for assessing the extent and pattern of cytosine methylation and has been applied to genomes of several species (Arabidopsis, grape, maize, tomato, and pepper)...
  70. pmc Hemorrhagic hypotension-induced hypersensitivity of vagal pulmonary C-fibers to chemical stimulation and lung inflation in anesthetized rats
    Ruei Lung Lin
    Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky and
    Am J Physiol Regul Integr Comp Physiol 308:R605-13. 2015
    ..from the femoral arterial catheter into a blood reservoir and lowering the mean systemic arterial pressure (MSAP) to ∼40 mmHg for 20 min. Our results showed the following...
  71. doi Efficient Fludarabine-Activating PNP From Archaea as a Guidance for Redesign the Active Site of E. Coli PNP
    Giovanna Cacciapuoti
    Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via L De Crecchio 7, Naples, 80138, Italy
    J Cell Biochem 117:1126-35. 2016
    ..coli PNP, SsMTAP, and SsMTAPII allowed to identify the crucial residues involved in the binding with this substrate, and provided structural information to improve the catalytic efficiency of E. coli PNP by enzyme redesign...
  72. pmc Long-term Potentiation at Temporoammonic Path-CA1 Synapses in Freely Moving Rats
    Jossina Gonzalez
    Department of Biology, University of Texas at San Antonio San Antonio, TX, USA
    Front Neural Circuits 10:2. 2016
    ..stimulation of the medial aspect of the angular bundle and investigated LTP at medial temporoammonic path (mTAP)-CA1 synapses in freely moving rats...
  73. doi Endoscope-assisted medial sural artery perforator flap for head and neck reconstruction
    Xiang qian Shen
    Department of Hand Surgery and Microsurgery Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
    J Plast Reconstr Aesthet Surg 69:1059-65. 2016
    ..We recommend a technique of using endoscopy to confirm the location of the medial sural artery perforator (MSAP). Further, we use a free MSAP flap for the reconstruction of head and neck defects.
  74. doi Social insurance preliminary agreement (MSAP) to enter PRM ward for patients after orthopedic surgery in Gonesse hospital
    Patricia Ribinik
    Centre Hospitalier de Gonesse, Médecine physique et de réadaptation, Gonesse cedex, France Electronic address
    Ann Phys Rehabil Med 59:e47. 2016
    ..To evaluate the interest of social insurance preliminary agreement to enter PRM ward for patients after orthopedic surgery in Gonesse hospital...
  75. pmc Use of Combined MSAP and NGS Techniques to Identify Differentially Methylated Regions in Somaclones: A Case Study of Two Stable Somatic Wheat Mutants
    Miroslav Baránek
    Mendeleum Department of Genetics, Horticulture Faculty of Mendel University in Brno, Lednice, Czech Republic
    PLoS ONE 11:e0165749. 2016
    ..We used a novel approach consisting of a standard MSAP analysis followed by deep amplicon sequencing to better understand this phenomenon...
  76. pmc Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5'-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway
    Juliana Roberta Torini
    Laboratório de Biologia Estrutural, Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos, SP, Brazil
    PLoS Negl Trop Dis 10:e0005178. 2016
    ..In the present work we show that S. mansoni 5´-deoxy-5´-methylthioadenosine phosphorylase (MTAP, E.C. 2.4.2.28) is capable of use adenosine as a substrate to the production of adenine...
  77. ncbi Molecular cloning of microtubule-associated protein 1 (MAP1A) and microtubule-associated protein 5 (MAP1B): identification of distinct genes and their differential expression in developing brain
    C C Garner
    Friedrich Miescher Institute, Basel, Switzerland
    J Neurochem 55:146-54. 1990
    ..By analysis of hybrid mouse-hamster cell lines, the MAP1 gene was located to mouse chromosome 2, designated Mtap-1, and the MAP5 gene to chromosome 13, designated Mtap-5...
  78. ncbi Prevention of thromboxane A2 receptor-mediated pulmonary hypertension by a nonpeptide angiotensin II type 1 receptor antagonist
    F Bertolino
    Division of Cardiovascular Diseases II, Centre de Recherche Pierre Fabre, Castres, France
    J Pharmacol Exp Ther 268:747-52. 1994
    ..In anesthetized, open chest rats, U-46619 dose dependently increased MPAP whereas MSAP presented a biphasic evolution. The U-446619 (1.25 micrograms/kg)-increased MPAP (52.4 +/- 12.1%; P < ...
  79. ncbi The structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase at 1.7 A resolution provides insights into substrate binding and catalysis
    T C Appleby
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA
    Structure 7:629-41. 1999
    5'-Deoxy-5'-methylthioadenosine phosphorylase (MTAP) catalyzes the reversible phosphorolysis of 5'-deoxy-5'-methylthioadenosine (MTA) to adenine and 5-methylthio-D-ribose-1-phosphate...
  80. ncbi ZD1611, an orally active endothelin-A receptor antagonist, prevents chronic hypoxia-induced pulmonary hypertension in the rat
    R A Bialecki
    Respiratory, Inflammatory and Neurological Diseases Research Section, Zeneca Pharmaceuticals, Wilmington, Delaware, 19850 5437, USA
    Pulm Pharmacol Ther 12:303-12. 1999
    ..Hypoxic exposure did not change mean systemic arterial pressure (MSAP). ZD1611 did not affect MSAP, plasma ET-1 concentrations, or blood gases measured when rats respired room air...
  81. ncbi Methylthioadenosine phosphorylase gene deletions are common in osteosarcoma
    José M García-Castellano
    Orthopaedic Surgery Service, affiliated with Weil Medical College of Cornell University, New York, NY 10021, USA
    Clin Cancer Res 8:782-7. 2002
    Methylthioadenosine phosphorylase (MTAP) is an enzyme essential in the salvage of cellular adenine and methionine synthesis...
  82. ncbi Detection of gene amplification and deletion in high-grade gliomas using a genome DNA microarray (GenoSensor Array 300)
    Teruo Sasaki
    Department of Neurosurgery, Iwate Medical University, 19 1 Uchimaru, Morioka, Iwate 020 8505, Japan
    Brain Tumor Pathol 20:59-63. 2003
    ..A high frequency of deleted genes was observed in 6 of 11 cases (54.5%), including FGFR2, MTAP, and DMBT1...
  83. ncbi MSAP, the meichroacidin homolog of carp (Cyprinus carpio), differs from the rodent counterpart in germline expression and involves flagellar differentiation
    Tsai Kai Ju
    Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
    Biol Reprod 71:1419-29. 2004
    ..In this study, a male-specific homolog of the meichroacidin gene, called MSAP (MORN motif-containing sperm-specific axonemal protein), was isolated and further characterized...
  84. ncbi Genetic analysis of human glioblastomas using a genomic microarray system
    Tsuyoshi Suzuki
    Department of Neurosurgery, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Brain Tumor Pathol 21:27-34. 2004
    ..3%), ABCB1 (MDR1) (36.7%), and PDGFRA (26.7%). Genes that were frequently deleted included (56.7%), FGFR2 (66.7%), MTAP (60.0%), DMBT1 CDKN2A (p16)/MTAP (50.0%), PIK3CA (43.3%), and EGR2 (43...
  85. ncbi Detection of stool DNA mutations before and after treatment of colorectal neoplasia
    Sapna Syngal
    Division of Population Sciences, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Cancer 106:277-83. 2006
    ....
  86. ncbi Acute vasodilator effect of fasudil, a Rho-kinase inhibitor, in monocrotaline-induced pulmonary hypertension in rats
    Bao Hua Jiang
    Department of Cardiovascular Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    J Cardiovasc Pharmacol 49:85-9. 2007
    ..a middle dose (10 mg/kg) caused a significant reduction in mPAP without change in mean systemic arterial pressure (mSAP), and a high dose (30 mg/kg) significantly reduced both mPAP and mSAP...
  87. ncbi A transition state analogue of 5'-methylthioadenosine phosphorylase induces apoptosis in head and neck cancers
    Indranil Basu
    Biochemistry, Pathology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA
    J Biol Chem 282:21477-86. 2007
    Methylthio-DADMe-immucillin-A (MT-DADMe-ImmA) is an 86-pm inhibitor of human 5'-methylthioadenosine phosphorylase (MTAP). The sole function of MTAP is to recycle 5'-methylthioadenosine (MTA) to S-adenosylmethionine...
  88. ncbi Analysis on DNA methylation of various tissues in chicken
    Qing Xu
    Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China
    Anim Biotechnol 18:231-41. 2007
    ..patterns of CCGG sites were detected with fluorescent-labeled methylation sensitive amplified polymorphism (F-MSAP) in three chicken strains (White Leghorn, White Plymouth Rock, and their F(1) hybrids)...
  89. doi Azetidine based transition state analogue inhibitors of N-ribosyl hydrolases and phosphorylases
    Gary B Evans
    Carbohydrate Chemistry Team, Industrial Research Limited, PO Box 31310, Lower Hutt, New Zealand
    J Med Chem 51:948-56. 2008
    ..inhibitors (equilibrium dissociation constants as low as 229 pM) of purine nucleoside phosphorylase (PNP), methylthioadenosine phosphorylase (MTAP), and methylthioadenosine nucleosidase (MTAN), with potential utility as drug candidates.
  90. doi Chromosomal imbalances and partial uniparental disomies in primary central nervous system lymphoma
    H Schwindt
    Department of Neuropathology, University Hospital of Cologne, Cologne D 50924, Germany
    Leukemia 23:1875-84. 2009
    ..3. These genomic alterations affected the HLA locus, the CDKN2A/p16, CDKN2B/p15 and MTAP, as well as the PRDM1, FAS, MALT1, and BCL2 genes...
  91. pmc Mice heterozygous for germ-line mutations in methylthioadenosine phosphorylase (MTAP) die prematurely of T-cell lymphoma
    Yuwaraj Kadariya
    Division of Population Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Cancer Res 69:5961-9. 2009
    Large homozygous deletions of 9p21 that inactivate CDKN2A, ARF, and MTAP are common in a wide variety of human cancers...
  92. doi CDKN2A and MTAP deletions in peritoneal mesotheliomas are correlated with loss of p16 protein expression and poor survival
    Alyssa M Krasinskas
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213 2546, USA
    Mod Pathol 23:531-8. 2010
    ..b>MTAP resides in the same gene cluster of the 9p21 region and is co-deleted in the majority of CDKN2A deleted cases...
  93. ncbi Tumor suppressor gene alterations of spontaneously malignant transformed cells from human embryonic muscle in vitro
    Xianyao Wang
    Central Laboratory, Chaozhou Central Hospital, Chaozhou 521021, PR China
    Oncol Rep 24:555-61. 2010
    ..several tumor suppressor genes of these cell lines, and homozygous deletions within chromosomal band 9p2l including MTAP (methylthioadenosine phosphorylase), p16 and p15 were detected...
  94. pmc Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing
    Rong Guan
    Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, United States
    Biochemistry 50:1247-54. 2011
    The PA3004 gene of Pseudomonas aeruginosa PAO1 was originally annotated as a 5'-methylthioadenosine phosphorylase (MTAP)...
  95. doi Heritable alteration in DNA methylation induced by nitrogen-deficiency stress accompanies enhanced tolerance by progenies to the stress in rice (Oryza sativa L.)
    H P Kou
    Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
    J Plant Physiol 168:1685-93. 2011
    ..b>MSAP analysis revealed that locus-specific methylation alteration occurred in leaf-tissue of the stressed plants (S(0)) ..
  96. doi Assessment of genetic and epigenetic variation during long-term Taxus cell culture
    Chunhua Fu
    Department of Biotechnology, College of Life Science and Technology, Institute of Resource Biology and Biotechnology, Huazhong University of Science and Technology, 430074 Wuhan, China
    Plant Cell Rep 31:1321-31. 2012
    ..during culture using amplified fragment-length polymorphism (AFLP), methylation-sensitive amplified polymorphism (MSAP), and high-performance liquid chromatography (HPLC) analyses...
  97. pmc The MTAP-CDKN2A locus confers susceptibility to a naturally occurring canine cancer
    Abigail L Shearin
    Cancer Genetics Branch, National Human Genome Research Institute, NIH, Bethesda, MD 20892, USA
    Cancer Epidemiol Biomarkers Prev 21:1019-27. 2012
    ..Cancers that have been difficult to study in human families or populations are of particular interest. Histiocytic sarcoma is a rare and poorly understood neoplasm in humans that occurs in 15% to 25% of Bernese Mountain Dogs (BMD)...
  98. pmc Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway
    Camille Mary
    CALIPHO group, SIB Swiss Institute of Bioinformatics, University of Geneva, Geneva, Switzerland
    PLoS ONE 7:e52877. 2012
    ..the methionine salvage pathway takes place in the cytosol and usually involves six enzymatic activities: MTA phosphorylase (MTAP, EC 2.4.2.28), 5'-methylthioribose-1-phosphate isomerase (mtnA, EC 5.3.1...
  99. doi The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family
    Sachin Kumar Gupta
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    FEBS Lett 587:1399-404. 2013
    ..of the C/D small nucleolar RNA (snoRNA) family and associates with the methyltransferase-associated protein (MTAP), whose homologue binds to mammalian TER. Silencing of TbTER resulted in telomere shortening...
  100. doi Scoring and analysis of methylation-sensitive amplification polymorphisms for epigenetic population studies
    Benjamin Schulz
    Institute of Landscape Ecology and Resource Management, Research Centre for BioSystems, Land Use and Nutrition IFZ, Justus Liebig University Giessen, Giessen, Germany
    Mol Ecol Resour 13:642-53. 2013
    ..evolutionary context, epigenetic studies are increasingly using methylation-sensitive amplification polymorphism (MSAP) for surveys at the population or species level...
  101. doi Epigenetic control of heavy metal stress response in mycorrhizal versus non-mycorrhizal poplar plants
    Angela Cicatelli
    Dipartimento di Chimica e Biologia, Universita di Salerno, 84084, Fisciano, SA, Italy
    Environ Sci Pollut Res Int 21:1723-37. 2014
    ..in a greenhouse on multimetal polluted or unpolluted soil, the Methylation Sensitive Amplification Polymorphism (MSAP) approach was adopted to detect cytosine DNA methylation...

Research Grants15

  1. Vern L Schramm; Fiscal Year: 2016
    ..is an orally available transition state analogue inhibitor for human 5'- methylthioadenosine phosphorylase (MTAP)...
  2. Purine salvage as a chemotherapeutic target in malaria
    Kami Kim; Fiscal Year: 2002
    ..The significance of alternative purine salvage enzymes methylthioadenosine phosphorylase (MTAP) and adenine phosphoribosyltransferase (APRT) will be examined using genetic disruption and inhibitor ablation of ..
  3. The Role of Methylthioadenosine Phosphorylase Loss in Tumorigenesis
    Warren D Kruger; Fiscal Year: 2013
    Methylthioadenosine phosphorylase (MTAP) is a key enzyme in the methionine salvage pathway whose function is to convert 5cent-deoxy-5cent-methylthioadenosine (MTA) into methionine...
  4. Colon Polyp Surveillance with DNA Analysis of Stool
    Philip Schoenfeld; Fiscal Year: 2003
    ..Multi-target DNA-based assay panels (MTAP) of stool may accurately diagnose colon adenomas...
  5. Reagents for the Design of Targeted Multifunctional Nanomaterials
    Lee Josephson; Fiscal Year: 2012
    ..multifunctional materials design lies in a new class of reagents termed Multifunctional Single Attachment Point or MSAP's...
  6. CHROMOSOME 9P ABERRATIONS IN GLIOMA
    Dennis Carson; Fiscal Year: 1999
    ..using a newly developed microtiter format gene dosage assay; (3) to use our existent antibodies against MTA phosphorylase to quantitate levels of the enzyme in extracts of primary gliomas; (4) to correlate the molecular genetic ..
  7. CHARACTERIZATION OF A TUMOR SUPPRESSOR GENE ON 9P
    Olufunmilayo Olopade; Fiscal Year: 1999
    ..The applicant has cloned the MTAP gene and other transcription units in the critical region on 9p2l...
  8. Mechanism of Action of Folate Antagonist
    Joseph Bertino; Fiscal Year: 2008
    ..The use of MEF cells null for p53, p19 ARF and both will be utilized to test in a more well defined system the function of these proteins on DHFR levels. ..
  9. PERTUSSIS, DIPHTHERIA, CHOLERA TOXINS-INHIBITOR DESIGN
    Vern Schramm; Fiscal Year: 2003
    ..The goal of this work is to extend transition state inhibitor design to the complex reactions catalyzed by bacterial ADP-ribosylating toxins. ..
  10. TRANSITION STATE ANALYSIS OF ENZYMATIC REACTIONS
    Vern Schramm; Fiscal Year: 2007
    ..unreadable]..
  11. BENIGN BREAST DISEASE--MOLECULAR DIFFERENTIATION OF RISK
    Maria Worsham; Fiscal Year: 2001
    ....
  12. INHERITED MSH6 MUTATIONS IN DIVERSE COLORECTAL CANCERS
    Sapna Syngal; Fiscal Year: 2004
    ..Evidence that MSH6 is a moderately penetrant, later-onset hereditary colorectal cancer gene would imply the existence of similar genes for other common cancers that await discovery. ..
  13. ASPL TFE3 fusion in human cancers
    Marc Ladanyi; Fiscal Year: 2006
    ..3->qter; and finally analyze selected candidate genes in the same region for inactivating mutations. ..
  14. MOLECULAR MODELING OF DIAGNOSIS AND PROGNOSIS IN HNSCC
    Maria Worsham; Fiscal Year: 2009
    ..abstract_text> ..