MK2

Summary

Gene Symbol: MK2
Description: mitogen-activated protein kinase-activated protein kinase 2
Alias: MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2
Species: human

Top Publications

  1. pmc Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB
    M Deak
    MRC Protein Phosphorylation Unit, Departments of Biochemistry, Anatomy and Physiology, University of Dundee, Scotland
    EMBO J 17:4426-41. 1998
  2. ncbi Phosphorylation and activation of human tyrosine hydroxylase in vitro by mitogen-activated protein (MAP) kinase and MAP-kinase-activated kinases 1 and 2
    C Sutherland
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 217:715-22. 1993
  3. pmc FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2
    Y Tan
    Cell Signaling Laboratory, New England Biolabs, Beverly, MA 01915, USA
    EMBO J 15:4629-42. 1996
  4. ncbi Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2)
    Oliver Werz
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 277:14793-800. 2002
  5. doi MK2 posttranscriptionally regulates TNF-α-induced expression of ICAM-1 and IL-8 via tristetraprolin in human pulmonary microvascular endothelial cells
    Jia Xin Shi
    Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing University School of Medicine, China
    Am J Physiol Lung Cell Mol Physiol 302:L793-9. 2012
  6. pmc 5-lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases
    O Werz
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II Karolinska Institutet, S 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 97:5261-6. 2000
  7. pmc Human immunodeficiency virus type 1 Vpr induces cell cycle G2 arrest through Srk1/MK2-mediated phosphorylation of Cdc25
    Sylvain Huard
    Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, MSTF700A, Baltimore, MD 21201, USA
    J Virol 82:2904-17. 2008
  8. ncbi Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins
    D Stokoe
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 313:307-13. 1992
  9. pmc Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation
    K Engel
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    EMBO J 17:3363-71. 1998
  10. ncbi MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis
    A Kotlyarov
    Martin Luther University Halle Wittenberg, Germany
    Nat Cell Biol 1:94-7. 1999

Research Grants

  1. Small heat shock proteins in smooth muscle plasticity
    William T Gerthoffer; Fiscal Year: 2013
  2. Mechanism of Anthrax Lethal Factor Toxicity
    Usamah S Kayyali; Fiscal Year: 2012
  3. MAP Kinase Signaling in Apoptosis-Induced Ventilator-Associated Lung Injury
    Mahendra Damarla; Fiscal Year: 2013
  4. SANDRA ANN REMPEL; Fiscal Year: 2015
  5. MOLECULAR MODELS OF IMMUNE-MEDIATED VASCULAR INJURY
    Jeffrey R Bender; Fiscal Year: 2012
  6. Michael B Yaffe; Fiscal Year: 2016
  7. Regulation of Neuronal Survival by the Rit GTPase
    DOUGLAS ALLEN ANDRES; Fiscal Year: 2012
  8. Hypoxia-induced Endothelial Barrier Dysfunction
    Usamah Kayyali; Fiscal Year: 2009
  9. Post-transcriptional control of inflammatory gene expression
    Thomas A Hamilton; Fiscal Year: 2013
  10. Mechanism of Myocardial Angiogenesis in Transgenic/Knockout Animals
    Nilanjana Maulik; Fiscal Year: 2010

Detail Information

Publications259 found, 100 shown here

  1. pmc Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB
    M Deak
    MRC Protein Phosphorylation Unit, Departments of Biochemistry, Anatomy and Physiology, University of Dundee, Scotland
    EMBO J 17:4426-41. 1998
    ..These findings, together with other observations, suggest that MSK1 may mediate the growth-factor and stress-induced activation of CREB...
  2. ncbi Phosphorylation and activation of human tyrosine hydroxylase in vitro by mitogen-activated protein (MAP) kinase and MAP-kinase-activated kinases 1 and 2
    C Sutherland
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 217:715-22. 1993
    ..The effect on activity of phosphorylating both Ser31 and Ser40 was not additive. The possible roles of MAPKAP kinase-1, MAPKAP kinase-2 and MAP kinase in the regulation of tyrosine hydroxylase in vivo are discussed...
  3. pmc FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2
    Y Tan
    Cell Signaling Laboratory, New England Biolabs, Beverly, MA 01915, USA
    EMBO J 15:4629-42. 1996
    ..These findings identify a new growth factor and stress-activated signaling pathway that regulates gene expression at the CRE...
  4. ncbi Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2)
    Oliver Werz
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 277:14793-800. 2002
    ..Here we show that mutation of Ser-271 to Ala in 5-LO abolished MK2 catalyzed phosphorylation and clearly reduced phosphorylation by kinases prepared from stimulated polymorphonuclear ..
  5. doi MK2 posttranscriptionally regulates TNF-α-induced expression of ICAM-1 and IL-8 via tristetraprolin in human pulmonary microvascular endothelial cells
    Jia Xin Shi
    Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing University School of Medicine, China
    Am J Physiol Lung Cell Mol Physiol 302:L793-9. 2012
    Tristetraprolin (TTP), a substrate of p38 mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2), is an RNA-binding protein that binds to AU-rich elements (AREs) in the 3'-untranslated region (3'-UTR) of its target ..
  6. pmc 5-lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases
    O Werz
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II Karolinska Institutet, S 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 97:5261-6. 2000
    ..immunoreactivity, one of these was identified as mitogen-activated protein kinase-activated protein kinase 2 (MAPKAP kinase 2)...
  7. pmc Human immunodeficiency virus type 1 Vpr induces cell cycle G2 arrest through Srk1/MK2-mediated phosphorylation of Cdc25
    Sylvain Huard
    Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, MSTF700A, Baltimore, MD 21201, USA
    J Virol 82:2904-17. 2008
    ..Consistent with our findings in fission yeast, depletion of the MK2 gene, a human homologue of Srk1, either by small interfering RNA or an MK2 inhibitor suppresses Vpr-induced cell ..
  8. ncbi Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins
    D Stokoe
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 313:307-13. 1992
    ..These results suggest that MAPKAP kinase-2 is the enzyme responsible for the phosphorylation of these small heat shock proteins in mammalian cells...
  9. pmc Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation
    K Engel
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    EMBO J 17:3363-71. 1998
    To study the intracellular localization of MAPKAP kinase 2 (MK2), which carries a putative bipartite nuclear localization signal (NLS), we constructed a green fluorescent protein-MAPKAP kinase 2 fusion protein (GFP-MK2)...
  10. ncbi MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis
    A Kotlyarov
    Martin Luther University Halle Wittenberg, Germany
    Nat Cell Biol 1:94-7. 1999
    b>MAPKAP kinase 2 (MK2) is one of several kinases that are regulated through direct phosphorylation by p38 MAP kinase. By introducing a targeted mutation into the mouse MK2 gene, we have determined the physiological function of MK2 in vivo...
  11. ncbi The p38 pathway provides negative feedback for Ras proliferative signaling
    G Chen
    Cleveland Clinic Foundation, Department of Molecular Biology, Cleveland, Ohio 44195, USA
    J Biol Chem 275:38973-80. 2000
    ..Oncogenic Ras activated p38 and two p38-activated protein kinases, MAPK-activated protein kinase 2 (MK2) and p38-related/activated protein kinase (PRAK)...
  12. ncbi MAPKAPK2 and HSP27 are downstream effectors of p38 MAP kinase-mediated matrix metalloproteinase type 2 activation and cell invasion in human prostate cancer
    L Xu
    Department of Medicine, Division of Hematology Oncology, Northwestern University Medical School, Chicago, IL, USA
    Oncogene 25:2987-98. 2006
    ..This study demonstrates that both MAPKAPK2 and HSP27 are necessary for TGFbeta-mediated increases in MMP-2 and cell invasion in human prostate cancer...
  13. pmc Molecular basis of MAPK-activated protein kinase 2:p38 assembly
    Andre White
    Department of Medicinal Chemistry, Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, CT 06877, USA
    Proc Natl Acad Sci U S A 104:6353-8. 2007
    p38 MAPK and MAPK-activated protein kinase 2 (MK2) are key components of signaling pathways leading to many cellular responses, notably the proinflammatory cytokine production...
  14. ncbi Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of Hsp27
    N W Freshney
    Department of Development and Signalling, Babraham Institute, Cambridge, England
    Cell 78:1039-49. 1994
    ..The substrate specificity of p40 differed from that of p42 and p54 MAP kinases. The upstream activator was not a MAP kinase kinase. p50 resembled MAPKAPK-2 and may be identical...
  15. ncbi Affinity purification of ARE-binding proteins identifies polyA-binding protein 1 as a potential substrate in MK2-induced mRNA stabilization
    Frank Bollig
    Institute of Pharmacology, Medical School Hannover, Germany
    Biochem Biophys Res Commun 301:665-70. 2003
    ..response to external signals which activate the p38 MAP kinase cascade including the p38 MAP kinase substrate MAPKAP kinase 2 (MK2)...
  16. doi MAPKAP kinase 2 overexpression influences prognosis in gastrointestinal stromal tumors and associates with copy number variations on chromosome 1 and expression of p38 MAP kinase and ETV1
    Peter Birner
    Departments of Surgery and Obstetrics and Gynecology, Clinical Institute of Pathology, Institute of Neurology, Medical University of Vienna, Vienna, Austria
    Clin Cancer Res 18:1879-87. 2012
    ..ETV1 has been proposed to be activated by KIT mutations in gastrointestinal stromal tumors (GIST). The aim of the study was to evaluate the clinical role of ETV1 and associated proteins in GIST...
  17. ncbi The abnormal occurrence and the differentiation-dependent distribution of N-acetyl and N-glycolyl species of the ganglioside GM2 in human germ cell tumors. A study with specific monoclonal antibodies
    M Miyake
    Department of Laboratory Medicine and Clinical Science, School of Medicine, Kyoto University, Japan
    Cancer 65:499-505. 1990
    ..can distinguish the molecular species of the sialic acid moiety: the antibody MK1-16 is specific for N-acetyl GM2, MK2-34 is specific for N-glycolyl GM2, and MK1-17 detects both N-acetyl and N-glycolyl GM2...
  18. pmc MAPKAPK-2 modulates p38-MAPK localization and small heat shock protein phosphorylation but does not mediate the injury associated with p38-MAPK activation during myocardial ischemia
    Diana A Gorog
    Cardiovascular Division, Department of Cardiology, King s College London School of Medicine, The Rayne Institute, St Thomas s Hospital, London SE1 7EH, UK
    Cell Stress Chaperones 14:477-89. 2009
    MAPKAPK-2 (MK2) is a protein kinase activated downstream of p38-MAPK which phosphorylates the small heat shock proteins HSP27 and alphaB crystallin and modulates p38-MAPK cellular distribution...
  19. pmc Dynasore, a dynamin inhibitor, inhibits Trypanosoma cruzi entry into peritoneal macrophages
    Emile S Barrias
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS Bloco G, Ilha do Fundao, Rio de Janeiro, Brasil
    PLoS ONE 5:e7764. 2010
    ..Among these, the GTPase dynamin plays an important role in clathrin-mediated endocytosis, and it was recently found that dynamin can participate in a phagocytic process...
  20. pmc Bovine parainfluenza virus type 3 (BPIV3) fusion and hemagglutinin-neuraminidase glycoproteins make an important contribution to the restricted replication of BPIV3 in primates
    A C Schmidt
    Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 74:8922-9. 2000
    ..The chimeric viruses were readily recovered and replicated in simian LLC-MK2 cells to a level comparable to that of their parental viruses, suggesting that the heterologous glycoproteins were ..
  21. doi Determinants that control the distinct subcellular localization of p38alpha-PRAK and p38beta-PRAK complexes
    Qinxi Li
    The Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China
    J Biol Chem 283:11014-23. 2008
    ..The subcellular localization of MK2 (MAPK-activated protein kinase 2), another downstream kinase of p38, was regulated in the same manner as that of ..
  22. pmc Distinct cellular functions of MK2
    Alexey Kotlyarov
    Institute of Biochemistry, Medical School Hannover, Hannover 30625, Germany
    Mol Cell Biol 22:4827-35. 2002
    Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2) is activated upon stress by p38 MAPK alpha and -beta, which bind to a basic docking motif in the C terminus of MK2 and which subsequently phosphorylate its ..
  23. pmc Cloning of regions required for contact hemolysis and entry into LLC-MK2 cells from Shigella sonnei form I plasmid: virF is a positive regulator gene for these phenotypes
    J Kato
    Department of Bacteriology, National Institute of Health, Tokyo, Japan
    Infect Immun 57:1391-8. 1989
    Two distinct regions required for both contact hemolysis and entry into LLC-MK2 cells were cloned into Escherichia coli from the Shigella sonnei form I plasmid, pSS120. The first region was cloned into an E...
  24. ncbi Differential regulation of TNFalpha and GM-CSF induced activation of P38 MAPK in neutrophils and eosinophils
    Willem ten Hove
    Department of Pulmonary Diseases, University Medical Center Utrecht UMCU, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Mol Immunol 44:2492-6. 2007
    ..In this study P38 MAPK phosphorylation and activation (measured via phosphorylation of P38 MAPK downstream target MK2) were investigated upon engagement of the GM-CSF- and TNFalpha-receptors expressed on both eosinophils and ..
  25. pmc Recovery of a fully viable chimeric human parainfluenza virus (PIV) type 3 in which the hemagglutinin-neuraminidase and fusion glycoproteins have been replaced by those of PIV type 1
    T Tao
    Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0720, USA
    J Virol 72:2955-61. 1998
    ..replacement chimeras of vesicular somatitis virus or measles virus, the PIV3-1 chimera replicates in LLC-MK2 cells and in the respiratory tract of hamsters as efficiently as its PIV1 and PIV3 parents...
  26. pmc Stimulation of the p38 mitogen-activated protein kinase pathway in neonatal rat ventricular myocytes by the G protein-coupled receptor agonists, endothelin-1 and phenylephrine: a role in cardiac myocyte hypertrophy?
    A Clerk
    Division of Biomedical Sciences, Imperial College School of Medicine, Charing Cross Campus, London W6 8RF, United Kingdom
    J Cell Biol 142:523-35. 1998
    ..These data are not consistent with a role for the p38-MAPK pathway in the immediate induction of the morphological changes of hypertrophy but suggest that it may be necessary over a longer period to maintain the response...
  27. ncbi Effects of sodium ferulate on amyloid-beta-induced MKK3/MKK6-p38 MAPK-Hsp27 signal pathway and apoptosis in rat hippocampus
    Ying Jin
    Department of Pharmacology, Jinzhou Medical College, Jinzhou 121001, China
    Acta Pharmacol Sin 27:1309-16. 2006
    ..To observe the effects of sodium ferulate (SF) on amyloid beta (Abeta)1-40-induced p38 mitogen-activated protein kinase (MAPK) signal transduction pathway and the neuroprotective effects of SF...
  28. pmc Evaluation of different continuous cell lines in the isolation of mumps virus by the shell vial method from clinical samples
    J Reina
    Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta UIB, Andrea Doria 55, 07014 Palma de Mallorca, Spain
    J Clin Pathol 54:924-6. 2001
    To compare prospectively the efficacy of the Vero, LLC-MK2, MDCK, Hep-2, and MRC-5 cell lines in the isolation of the mumps virus from clinical samples by means of the shell vial method.
  29. doi GluR6-containing KA receptor mediates the activation of p38 MAP kinase in rat hippocampal CA1 region during brain ischemia injury
    Juan Chen
    Research Center for Biochemistry and Molecular Biology, Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, People s Republic of China
    Hippocampus 19:79-89. 2009
    ....
  30. ncbi The role of cellular senescence in Werner syndrome: toward therapeutic intervention in human premature aging
    Terence Davis
    Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Ann N Y Acad Sci 1100:455-69. 2007
    ..binding properties, specificities, and oral bioavailability, and of new potent and selective inhibitors of JNK and MK2, will make it possible to dissect the roles of various kinase pathways in the accelerated senescence of WS cells...
  31. pmc Mitogen-activated protein kinase-activated protein kinase 2 in angiotensin II-induced inflammation and hypertension: regulation of oxidative stress
    Talin Ebrahimian
    Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec, Canada
    Hypertension 57:245-54. 2011
    ..We questioned whether mitogen-activated protein kinase-activated protein kinase 2 (MK2), a downstream target of p38 mitogen-activated protein kinase, is involved in angiotensin II-induced vascular ..
  32. doi Vascular endothelial growth factor induces heat shock protein (HSP) 27 serine 82 phosphorylation and endothelial tubulogenesis via protein kinase D and independent of p38 kinase
    Ian M Evans
    Centre for Cardiovascular Biology and Medicine, Department of Medicine, The Rayne Institute, University College London, 5 University Street, London WC1E 6JJ, United Kingdom
    Cell Signal 20:1375-84. 2008
    ....
  33. doi Do camels (Camelus dromedarius) play an epidemiological role in the spread of Shiga Toxin producing Escherichia coli (STEC) infection?
    A El-Sayed
    Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
    Trop Anim Health Prod 40:469-73. 2008
    ..same time, a Stx-specific PCR screening was performed for the isolates using the shiga toxin specific primers Mk1-Mk2. Vero cells were also used for shiga toxin neutralization assay...
  34. ncbi Antiviral and cytotoxicity evaluation of 3-nitro-3-deazauridine
    L B Allen
    Texas College of Osteopathic Medicine, Fort Worth 76107
    Antiviral Res 12:259-67. 1989
    ..comparable toxicity to ribavirin in KB cells, was 4-fold less toxic in WISH cells and 4-fold more toxic in LLC-MK2 cells. Overall, 3N-3DU is markedly less toxic than its parent nucleoside, 3DU...
  35. ncbi Repression of enhancer activity by the adenovirus E1A tumor protein(s) is mediated through a novel HeLa cell nuclear factor
    A E Reifel-Miller
    Department of Molecular Genetics, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285
    J Biol Chem 266:13873-82. 1991
    ..from the adenovirus major late promoter in the human kidney cell line, 293, and in a monkey kidney cell line, MK2. In addition, enhancer activity can be stimulated by the E1A gene products in these cells...
  36. ncbi Ischemic preconditioning involves dual cardio-protective axes with p38MAPK as upstream target
    Norbert Nagy
    FAHA Cardiovascular Research Center, University of Connecticut School of Medicine Farmington, Cardiovascular Research Institute, CT 06030 1110, USA
    J Mol Cell Cardiol 42:981-90. 2007
    ..indicates a crucial role of p38 MAP (mitogen-activated protein) kinase (p38MAPK) and its downstream target MAPKAP kinase 2 (MK2) in ischemic preconditioning (IPC)...
  37. ncbi Does the hemoglobin concentration in fetal blood interfere with the accuracy of fetal reflection pulse oximetry?
    G M Arikan
    Department of Obstetrics and Gynecology, University of Graz, Austria
    Fetal Diagn Ther 13:236-40. 1998
    ..Our goal was to investigate the influence of the fetal hemoglobin (Hb) concentration on the accuracy of reflection pulse oximetry...
  38. pmc Antibodies to the vitronectin receptor (integrin alpha V beta 3) inhibit binding and infection of foot-and-mouth disease virus to cultured cells
    A Berinstein
    Plum Island Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Greenport, New York 11944
    J Virol 69:2664-6. 1995
    ..Competition binding experiments between type A12 FMDV and coxsackievirus A9 using BHK-21 and LLC-MK2 cells revealed shared receptor specificity between these two viruses...
  39. ncbi Identification of the human melanoma-associated chondroitin sulfate proteoglycan antigen epitope recognized by the antitumor monoclonal antibody 763.74 from a peptide phage library
    M Geiser
    Novartis Pharma AG, Core Technology Area, Basel, Switzerland
    Cancer Res 59:905-10. 1999
    ..74 by an anti-idiotypic MK2-23 mouse:human chimeric mAb and by A375 melanoma cells expressing the antigen MCSP...
  40. ncbi Heat shock protein 1 and the mitogen-activated protein kinase 14 pathway are important for mouse trophoblast stem cell differentiation
    Quinton A Winger
    Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah 84322 5600, USA
    Biol Reprod 76:884-91. 2007
    ..These results suggest that HSPB1 and the MAPK14 pathway are important during TS cell differentiation...
  41. doi Protein phosphatase 2A carboxymethylation and regulatory B subunits differentially regulate mast cell degranulation
    Gregory Kranias
    School of Biomedical Sciences, Faculty of Health, University of Newcastle, Callaghan, NSW 2308, Australia
    Cell Signal 22:1882-90. 2010
    ..Moreover downregulation of the PP2A-Bα subunit resulted in decreased MK2 phosphorylation and degranulation, whilst downregulation of the PP2A-B'δ subunit enhanced p38 MAPK phosphorylation ..
  42. pmc Characterization of a rhesus monkey calicivirus representing a new genus of Caliciviridae
    Tibor Farkas
    Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    J Virol 82:5408-16. 2008
    ..The TV can be cultured in a monkey kidney cell line (LLC-MK2) with the appearance of typical cytopathic effect...
  43. doi [A comparative study of direct immunofluorescence, enzyme immunoassay, and culture for diagnosing metapneumovirus infection]
    Ignacio Calicó
    Unidad de Virologia, Servicio de Microbiologia, Hospital Universitario Vall d Hebron, Barcelona, Espana
    Enferm Infecc Microbiol Clin 27:322-5. 2009
    ..This study compares a direct fluorescent antibody (DFA) test, enzyme immunoassay (EIA), and shell-vial culture for diagnosing acute bronchiolitis in infants...
  44. ncbi Lithium stabilizes the CCAAT/enhancer-binding protein alpha (C/EBPalpha) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct inhibition of proteasomal activity
    Minsub Shim
    Cell Signaling and Cancer Group, Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh 27695 7633, USA
    J Biol Chem 278:19674-81. 2003
    ..In cycloheximide-treated BALB/MK2 keratinocytes we found that C/EBPalpha is a short-lived protein with a half-life of approximately 1 h...
  45. ncbi In vitro mechanisms of monoclonal antibody neutralization of alphaviruses
    J T Roehrig
    Division of Vector Borne Viral Diseases, Centers for Disease Control, Fort Collins, Colorado 80522
    Virology 165:66-73. 1988
    ..novel MAb that defined the E2f epitope appeared to enhance virus attachment to Vero cells, but not BHK-21 or LLC-MK2 cells, by stabilizing virus-cell interaction. This antibody did, however, efficiently neutralize virus infectivity...
  46. ncbi Effect of Ca2+ on morphogenesis of HVJ (Sendai virus) virion in LLC-MK2 cells: suppression of viral production
    M Kamata
    Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan
    Cell Struct Funct 19:315-23. 1994
    Production of HVJ (Sendai virus) virion is suppressed in the infected LLC-MK2 cells in the low Ca2+ condition. To clarify this phenomenon, the effect of Ca2+ on HVJ morphogenesis was examined under normal and low Ca2+ conditions...
  47. ncbi Airway recruitment of leukocytes in mice is dependent on alpha4-integrins and vascular cell adhesion molecule-1
    J E Chin
    Cell Biology and Inflammation Research and Drug Development Toxicology, Pharmacia and Upjohn, Kalamazoo, Michigan 49001, USA
    Am J Physiol 272:L219-29. 1997
    ..7 (MK2), specific for VCAM-1. VCAM-1 was upregulated on endothelial cells in lung tissue after OA inhalation...
  48. ncbi Sensitized mast cells migrate toward the antigen: a response regulated by p38 mitogen-activated protein kinase and Rho-associated coiled-coil-forming protein kinase
    T Ishizuka
    First Department of Internal Medicine, Gunma University School of Medicine, Faculty of Health Sciences, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    J Immunol 167:2298-304. 2001
    ..Both p38 MAPK activation and Rho-dependent activation of Rho-associated coiled-coil-forming protein kinase may be required for Fc(epsilon)RI-mediated cell migration...
  49. pmc Proteolytic processing and deubiquitinating activity of papain-like proteases of human coronavirus NL63
    Zhongbin Chen
    Department of Microbiology and Immunology, Loyola University Medical Center, 2160 South First Avenue, Bldg 105, Rm 3929, Maywood, IL 60153, USA
    J Virol 81:6007-18. 2007
    ..replicase nonstructural proteins (nsp3 and nsp4) and detected replicase proteins from HCoV-NL63-infected LLC-MK2 cells by immunofluorescence, immunoprecipitation, and Western blot assays...
  50. pmc The G2 p38-mediated stress-activated checkpoint pathway becomes attenuated in transformed cells
    Alexei Mikhailov
    Laboratory of Cell Regulation, Division of Molecular Medicine, New York State Department of Health, Wadsworth Center, P O Box 509, Albany, New York 12201 0509, USA
    Curr Biol 17:2162-8. 2007
    ..Because the primary substrate of p38, the MK2 kinase, is also transiently (1-2 hr) activated by anisomycin in both normal and cancer cells, checkpoint disruption ..
  51. doi PI3K accelerates, but is not required for, neutrophil chemotaxis to fMLP
    Bryan Heit
    Immunology Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, T2N 4N1, Canada
    J Cell Sci 121:205-14. 2008
    ..By contrast, p38-MAPK-inhibited cells, or cells lacking MK2, were unable to polarize in response to fMLP...
  52. doi The tuberous sclerosis gene products hamartin and tuberin are multifunctional proteins with a wide spectrum of interacting partners
    Margit Rosner
    Medical Genetics, Obstetrics and Gynecology, Medical University of Vienna, Wahringer Gurtel 18 20, Vienna, Austria
    Mutat Res 658:234-46. 2008
    ..AMPK, CaM, CRB3/PATJ, cyclin A, cyclins D1, D2, D3, Dsh, ERalpha, Erk, FoxO1, HERC1, HPV16 E6, HSCP-70, HSP70-1, MK2, NEK1, p27KIP1, Pam, PC1, PP2Ac, Rabaptin-5, Rheb, RxRalpha/VDR and SMAD2/3...
  53. doi Effect of Crotalus viridis viridis snake venom on the ultrastructure and intracellular survival of Trypanosoma cruzi
    Camila M Adade
    Laboratório de Biologia Celular e Ultraestrutura, Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Goes, Centro de Ciencias da Saude, Bloco I, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Rio de Janeiro, Brazil
    Parasitology 138:46-58. 2011
    ..The effects of the venom on the parasite intracellular cycle were also analysed. Pre-infected LLC-MK2 cells incubated with Cvv venom showed a 76-93% reduction in the number of parasites per infected cell and a 94-97...
  54. doi A single amino acid mutation at position 170 of human parainfluenza virus type 1 fusion glycoprotein induces obvious syncytium formation and caspase-3-dependent cell death
    Masahiro Takaguchi
    Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka, Japan
    J Biochem 149:191-202. 2011
    ..acid (TCM-Neu5Ac2en), exhibited remarkable syncytium formation and virus-induced cell death in LLC-MK2 cells but no difference in susceptibility for the sialidase inhibitor TCM-Neu5Ac2en from that of wild-type hPIV1 ..
  55. ncbi A retinoic acid-responsive gene, MK, found in the teratocarcinoma system. Heterogeneity of the transcript and the nature of the translation product
    M Tomomura
    Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan
    J Biol Chem 265:10765-70. 1990
    ..So far three classes of cDNA clones (MK1, MK2, and MK3) have been identified; they were different in the 5'-untranslated region but shared the rest of the ..
  56. ncbi Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin
    Masako Kawachi
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Hum Gene Ther 18:881-94. 2007
    ..of F protein, was transported to the cell surface and incorporated into new viruses released from HVJ-infected LLC-MK2 cells...
  57. doi In vitro and experimental therapeutic studies of the calcium channel blocker bepridil: detection of viable Leishmania (L.) chagasi by real-time PCR
    Juliana Q Reimão
    Laboratory of Applied Toxinology on Anti Parasitic Drugs, Department of Parasitology, Instituto Adolfo Lutz Avenida Dr Arnaldo, 351, 8 andar Cerqueira César, CEP 01246 902 São Paulo, SP, Brazil
    Exp Parasitol 128:111-5. 2011
    ..The mammalian cytotoxicity using LLC-MK2 cells resulted in an IC(50) value of 62.67 μM...
  58. pmc p38 MAP kinase and MAPKAP kinases MK2/3 cooperatively phosphorylate epithelial keratins
    Manoj B Menon
    Institute of Biochemistry, Medical School Hannover, Hannover 30625, Germany
    J Biol Chem 285:33242-51. 2010
    The MAPK-activated protein kinases (MAPKAP kinases) MK2 and MK3 are directly activated via p38 MAPK phosphorylation, stabilize p38 by complex formation, and contribute to the stress response...
  59. ncbi Expression of fluorescent genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): its application to parasite-vector biology
    Palmira Guevara
    Instituto de Biologia Experimental, Universidad Central de Venezuela, Caracas 1041A, Venezuela
    J Med Entomol 42:48-56. 2005
    ..In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T...
  60. pmc RIG-I-mediated activation of p38 MAPK is essential for viral induction of interferon and activation of dendritic cells: dependence on TRAF2 and TAK1
    Susie S Mikkelsen
    Institute of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK 8000 Aarhus C, Denmark
    J Biol Chem 284:10774-82. 2009
    ..p38 MAPK exerted its function independent of the MAPK-activated protein kinases MK2, MNK, and MSK1/2. We also observed that TRAF2 and TAK1 were essential for RIG-I-mediated activation of p38 MAPK...
  61. pmc Fuzzy logic analysis of kinase pathway crosstalk in TNF/EGF/insulin-induced signaling
    Bree B Aldridge
    Center for Cell Decision Processes, Cambridge, Massachusetts, United States of America
    PLoS Comput Biol 5:e1000340. 2009
    ..We uncover a relationship between MK2 and ERK pathways that might account for the previously identified pro-survival influence of MK2...
  62. doi The role of mitogen-activated protein kinase-activated protein kinase 2 in the p38/TNF-alpha pathway of systemic and cutaneous inflammation
    Arndt J Schottelius
    Bayer Schering Pharma AG, Berlin, Germany
    J Invest Dermatol 130:481-91. 2010
    Mitogen-activated protein kinase-activated protein kinase 2 (MK2) is a downstream molecule of p38, involved in the production of TNF-alpha, a key cytokine, and an established drug target for many inflammatory diseases...
  63. ncbi Differences in biofilm formation and aggregative adherence between beta-lactam susceptible and beta-lactamases producing P. mirabilis clinical isolates
    Elisabetta Nucleo
    Department of Morphological, Eidological and Clinical Sciences, Section of Microbiology, University of Pavia, Pavia, Italy
    New Microbiol 33:37-45. 2010
    ..The same isolates were screened for aggregative adherence onto monkey kidney cells (LLC-MK2). All strains studied were capable of biofilm formation, though at different levels...
  64. ncbi Identification of a new human coronavirus
    Lia van der Hoek
    Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Nat Med 10:368-73. 2004
    ..cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line...
  65. pmc Phosphorylation of lysophosphatidylcholine acyltransferase 2 at Ser34 enhances platelet-activating factor production in endotoxin-stimulated macrophages
    Ryo Morimoto
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, Hongo 7 3 1, Tokyo 113 0033, Japan
    J Biol Chem 285:29857-62. 2010
    ..LPCAT2 phosphorylation through LPS-TLR4 signaling may directly depend on MAPK-activated protein kinase 2 (MAPKAP kinase 2 or MK2)...
  66. pmc A novel role for MAPKAPK2 in morphogenesis during zebrafish development
    Beth A Holloway
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Genet 5:e1000413. 2009
    ..We postulate that a p38 MAPKAPK2 kinase cascade modulates the activity of F-actin at the yolk cell margin circumference allowing the gradual closure of the blastopore as epiboly progresses...
  67. ncbi Regulation of HSP60 and the role of MK2 in a new model of severe experimental pancreatitis
    Yong Yu Li
    Department of Pathophysiology, School of Medicine, Tongji University, Shanghai, China
    Am J Physiol Gastrointest Liver Physiol 297:G981-9. 2009
    The objective of this study was to investigate the role of MAPKAP kinase 2 (MK2) and heat shock protein (HSP) HSP60 in the pathogenesis of a new model of severe acute pancreatitis (AP)...
  68. ncbi Purification of cytochrome P-450 from polychlorinated biphenyl-treated crab-eating monkeys: high homology to a form of human cytochrome P-450
    K Ohta
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biochim Biophys Acta 996:142-5. 1989
    Cytochrome P-450, designated as P-450-MK2, was purified to an electrophoretic homogeneity from polychlorinated biphenyl (PCB)-treated female crab-eating monkeys...
  69. ncbi Stimulation of "stress-regulated" mitogen-activated protein kinases (stress-activated protein kinases/c-Jun N-terminal kinases and p38-mitogen-activated protein kinases) in perfused rat hearts by oxidative and other stresses
    A Clerk
    National Heart and Lung Institute Division, Imperial College School of Medicine, Royal Brompton Campus, London SW3 6LY, United Kingdom
    J Biol Chem 273:7228-34. 1998
    ..These data are consistent with a role for reactive oxygen species in the activation of SR-MAPKs during ischemia/reperfusion...
  70. ncbi Creation of a stress-activated p90 ribosomal S6 kinase. The carboxyl-terminal tail of the MAPK-activated protein kinases dictates the signal transduction pathway in which they function
    J A Smith
    Howard Hughes Medical Institute and the Markey Center for Cell Signaling, Departments of Medicine and Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 275:31588-93. 2000
    ..It is apparent that mechanisms controlling events downstream of the proline-directed MAPKs involve specific MAPK docking sites within the carboxyl termini of the MAPKAPKs that determine the cascade in which the MAPKAPK functions...
  71. pmc Human metapneumovirus detection in patients with severe acute respiratory syndrome
    Paul K S Chan
    Faculty of Medicine of the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Emerg Infect Dis 9:1058-63. 2003
    ..approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV...
  72. ncbi Identification of 4-anilino-3-quinolinecarbonitrile inhibitors of mitogen-activated protein/extracellular signal-regulated kinase 1 kinase
    Robert Mallon
    Oncology Research, Wyeth Research, 401 N Middletown Road, Pearl River, NY 10965, USA
    Mol Cancer Ther 3:755-62. 2004
    ..had minimal or no effect on Raf, epidermal growth factor receptor (EGFR), Akt, cyclin-dependent kinase 4 (CDK4), or MK2 kinases at concentrations >100-fold higher than those that inhibited MEK1 kinase...
  73. ncbi Involvement of p38 signaling pathway in interferon-alpha-mediated antiviral activity toward hepatitis C virus
    Hisashi Ishida
    Department of Molecular Therapeutics, Osaka University Graduate School of Medicine, 2 2, Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 321:722-7. 2004
    ..Treatment with the inhibitor of the mitogen-activated protein kinase-activated protein kinase 2 (MK2) showed the same level of reduction in the IFN-alpha-mediated anti-HCV activity as that with the p38 inhibitor...
  74. ncbi MAPKAP kinase-2 is a primary response gene induced by depolarization in PC12 cells and in brain
    Linda J Vician
    Department of Biological Chemistry, UCLA Center for the Health Sciences, Los Angeles, CA 90095 1570, USA
    J Neurosci Res 78:315-28. 2004
    ..MAPKAPK2 message is also induced in the dentate gyrus, CA1, and CA3 of the rat hippocampus between 2-4 hr after the onset of kainic acid-induced seizures...
  75. ncbi Detection of antibodies against human metapneumovirus by Western blot using recombinant nucleocapsid and matrix proteins
    Nobuhisa Ishiguro
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    J Med Virol 78:1091-5. 2006
    ..The results were compared with those of immunofluorescence assays (IFAs) based on hMPV-infected LLC-MK2 cells, which expressed the whole hMPV proteins. Thirty (61.2%) and 31 (63...
  76. doi Desferrioxamine (DFX) induces apoptosis through the p38-caspase8-Bid-Bax pathway in PHA-stimulated human lymphocytes
    Byeong Mo Kim
    School of Public Health, Seoul National University, 28 Yunkeun dong, Seoul 110 460, Korea
    Toxicol Appl Pharmacol 228:24-31. 2008
    ..Our results suggest that DFX-induced apoptosis is mediated by the p38 pathway and a caspase-8-dependent Bid-Bax pathway in human lymphocytes...
  77. doi HIV/gp120 decreases adult neural progenitor cell proliferation via checkpoint kinase-mediated cell-cycle withdrawal and G1 arrest
    Shu ichi Okamoto
    Center for Neuroscience, Stem Cells, and Aging, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Cell Stem Cell 1:230-6. 2007
    ..Our findings define a molecular mechanism that compromises adult neurogenesis in this neurodegenerative disorder...
  78. pmc Post-transcriptional regulation of TNF-induced expression of ICAM-1 and IL-8 in human lung microvascular endothelial cells: an obligatory role for the p38 MAPK-MK2 pathway dissociated with HSP27
    Xin Su
    Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China
    Biochim Biophys Acta 1783:1623-31. 2008
    ..Studies have shown that p38 mitogen-activated protein kinase (MAPK), MAPK-activated protein kinase 2 (MK2) and heat shock protein 27 (HSP27) are involved in the expression of pro-inflammatory mediators in other cell types...
  79. doi Host-cell-dependent role of actin cytoskeleton during the replication of a human strain of influenza A virus
    M C Arcangeletti
    Microbiology Section, Department of Pathology and Laboratory Medicine, University of Parma, Viale Antonio Gramsci 14, 43100 Parma, Italy
    Arch Virol 153:1209-21. 2008
    ..in the modulation of host permissiveness to A/NWS/33 human influenza virus infection in two mammalian (MDCK and LLC-MK2) cell lines in vitro...
  80. ncbi [Apoptosis of neuroglial cell and expressions of neurite outgrowth inhibitor B and MAP kinase-activated protein kinase 2 in white matter damage model of newborn rat brain]
    Qian Yang
    Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China
    Sichuan Da Xue Xue Bao Yi Xue Ban 39:202-6. 2008
    To observe and explore the apoptosis of neuroglial cell and the expression changes of neurite outgrowth inhibitor B (Nogo-B) and MAP kinase-activated protein kinase 2 (MK2) in SD rats with white matter damage (WMD).
  81. pmc Cytokine-induced signaling networks prioritize dynamic range over signal strength
    Kevin A Janes
    Koch Institute for Integrative Cancer Research, Center for Cell Decision Processes, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 135:343-54. 2008
    ..to analyze the mechanism of these failures, identifying new time- and stimulus-specific roles for Akt, ERK, and MK2 kinase activity in apoptosis, which were experimentally verified...
  82. pmc Role of MAPK kinase 6 in arthritis: distinct mechanism of action in inflammation and cytokine expression
    Toshio Yoshizawa
    Division of Rheumatology, Allergy and Immunology, University of California San Diego, School of Medicine, La Jolla, CA 92093, USA
    J Immunol 183:1360-7. 2009
    ..confocal microscopy experiments were performed to determine the subcellular location of MKK6, P-p38, and MAPKAPK2 (MK2). Arthritis scores were significantly lower in MKK6(-/-) mice compared with WT mice...
  83. pmc Human coronavirus NL63 open reading frame 3 encodes a virion-incorporated N-glycosylated membrane protein
    Marcel A Muller
    University of Bonn Medical Centre, Bonn, Germany
    Virol J 7:6. 2010
    ..Our study focuses on hCoV-NL63 open reading frame 3 (ORF 3) which is a highly conserved accessory protein among coronaviruses...
  84. doi Mechanisms regulating the nuclear translocation of p38 MAP kinase
    Xiaowei Gong
    Department of Pathophysiology, Key Laboratory of Proteomics of Gongdong Province, Southern Medical University, Guangzhou 510515, China
    J Cell Biochem 110:1420-9. 2010
    ..On the other hand, the nuclear export of p38 requires its dephosphorylation, and it is exported both in a MK2-dependent and a nuclear export signal (NES)-independent manner...
  85. pmc p38α MAPK is a MTOC-associated protein regulating spindle assembly, spindle length and accurate chromosome segregation during mouse oocyte meiotic maturation
    Xiang Hong Ou
    Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China
    Cell Cycle 9:4130-43. 2010
    ..by specific morpholino injection resulted in severely defective spindles and misaligned chromosomes probably via MK2 dephosphorylation...
  86. pmc Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells
    E Karaoz
    Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, Kocaeli, Turkey
    Cell Death Dis 1:e36. 2010
    ....
  87. ncbi A comprehensive siRNA screen for kinases that suppress macroautophagy in optimal growth conditions
    Piotr Szyniarowski
    Apoptosis Department and Centre for Genotoxic Stress Research, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
    Autophagy 7:892-903. 2011
    ..The depletion of MK2, PACSIN1, DAPK2, CDKL3 and SCYL1 functioned upstream of Akt-mTORC1 pathway, whereas CSNK1A1, BUB1, PKLR and NEK4 ..
  88. doi Successful propagation of Alkhumra (misnamed as Alkhurma) virus in C6/36 mosquito cells
    Tariq A Madani
    Department of Medicine, Faculty of Medicine, King Abdulaziz University, PO Box 80215, Jeddah 21589, Saudi Arabia
    Trans R Soc Trop Med Hyg 106:180-5. 2012
    ..The isolated virus was titrated in the mammalian rhesus monkey kidney cells (LLC-MK2)...
  89. doi A new type of pterocarpanquinone that affects Toxoplasma gondii tachyzoites in vitro
    Juliana de Araújo Portes
    Laboratório de Tecnologia em Cultura de Células, Centro Universitário Estadual da Zona Oeste UEZO Av Manuel Caldeira de Alvarenga, 1203, Campo Grande, Rio de Janeiro, RJ, CEP 23070 200, Brazil
    Vet Parasitol 186:261-9. 2012
    ..The aim of this work was to determine if this derivative is able to change T. gondii growth within LLC-MK2 cells. The drug did not arrest host cell growth, but was able to decrease the infection index of T...
  90. pmc Regulation of CCL5 expression in smooth muscle cells following arterial injury
    Huan Liu
    Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
    PLoS ONE 7:e30873. 2012
    ..The downstream molecule MK2 was required for p38-mediated CCL5 but not IP-10 inhibition...
  91. pmc MAPK usage in periodontal disease progression
    Qiyan Li
    Department of Endodontics, Periodontics and Oral Medicine, the First People s Hospital of Yunnan Province, Kunming, Yunnan 650032, China
    J Signal Transduct 2012:308943. 2012
    ..In this paper, we discuss evidence that the p38/MAPK-activated protein kinase-2 (MK2) signaling axis is needed for periodontal disease progression: an orally administered p38α inhibitor reduced the ..
  92. doi Comparison of frequency difference reconstruction algorithms for the detection of acute stroke using EIT in a realistic head-shaped tank
    B Packham
    Department of Medical Physics and Bioengineering, UCL, London, UK
    Physiol Meas 33:767-86. 2012
    ..background and a carrot-saline frequency-dependant background conductivity, in a head-shaped tank with the UCLH Mk2.5 MFEIT system...
  93. doi Understanding the molecular basis of MK2-p38α signaling complex assembly: insights into protein-protein interaction by molecular dynamics and free energy studies
    Ying Yang
    State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou 730000, China
    Mol Biosyst 8:2106-18. 2012
    The formation of a p38 MAPK and MAPK-activated protein kinase 2 (MK2) signaling complex is physiologically relevant to cellular responses such as the proinflammatory cytokine production...
  94. doi Fucosterol isolated from Undaria pinnatifida inhibits lipopolysaccharide-induced production of nitric oxide and pro-inflammatory cytokines via the inactivation of nuclear factor-κB and p38 mitogen-activated protein kinase in RAW264.7 macrophages
    Min Sang Yoo
    Department of Pharmaceutical Biochemistry, Kyung Hee University, Seoul, Republic of Korea
    Food Chem 135:967-75. 2012
    ..protein kinase kinases 3/6 (MKK3/6) and mitogen-activated protein kinase-activated protein kinase 2 (MK2), which are both involved in the p38 MAPK pathway...
  95. pmc Crucial roles of the protein kinases MK2 and MK3 in a mouse model of glomerulonephritis
    Adam J Guess
    Center for Clinical and Translational Research, The Research Institute at Nationwide Children s Hospital, Columbus, Ohio, United States of America
    PLoS ONE 8:e54239. 2013
    ..p38 MAPK signaling is partially mediated through MK2 and MK3, two phylogenetically related protein kinases that are its direct substrates...
  96. ncbi Mitogen-activated protein kinase-activated protein kinase 2 regulates tumor necrosis factor-induced interleukin-6 expression via human antigen R
    Jin Xu
    Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China
    Chin Med J (Engl) 126:4322-6. 2013
    ..In this study, we investigated whether mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2) and HuR participate in the tumor necrosis factor (TNF)-induced expression of interleukin-6 (IL-6).
  97. pmc Adaptive super-twisting observer for estimation of random road excitation profile in automotive suspension systems
    J J Rath
    School of Electronics Engineering, Kyungpook National University, Daegu 702 701, Republic of Korea
    ScientificWorldJournal 2014:203416. 2014
    ..Simulation results with Ford Fiesta MK2 demonstrate the effectiveness of the proposed observer for state and unknown input estimation for nonlinear active ..
  98. doi Ultrastructural aspects of Cystoisospora belli (syn. Isospora belli) in continuous cell lines
    Deisy V Resende
    Technics School of Health, Federal University of the Uberlândia, Umuarama, CEP 38400 902, Uberlandia, Minas Gerais, Brazil
    Microsc Res Tech 77:472-8. 2014
    ..This study aimed to describe the ultrastructure of endogenous stages of C. belli in Monkey Rhesus Kidney Cells (MK2) and Human Ileocecal Adenocarcinoma cells (HCT-8). Zoites of C...
  99. doi Arachidonic acid induces brain endothelial cell apoptosis via p38-MAPK and intracellular calcium signaling
    Justin Evans
    Signal Transduction Laboratory, Methodist Research Institute, Indiana University Health, Indiana University School of Medicine, Indianapolis, IN, USA
    Microvasc Res 98:145-58. 2015
    ....
  100. doi The MAPK-Activated Kinase MK2 Attenuates Dendritic Cell-Mediated Th1 Differentiation and Autoimmune Encephalomyelitis
    Klara Soukup
    Department of Immunology, St Anna Children s Cancer Research Institute, 1090 Vienna, Austria
    J Immunol 195:541-52. 2015
    ..In this study we show an opposing role of MK2, by which it consolidates immune regulatory functions in DCs through modulation of p38, ERK1/2-MAPK, and STAT3 ..
  101. doi Further evidence that naphthoquinone inhibits Toxoplasma gondii growth in vitro
    Luciana Lemos Rangel da Silva
    Laboratorio de Biologia Celular e Tecidual, Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, 28013 602, Brazil
    Parasitol Int 64:622-31. 2015
    ..gondii (RH strain) infected LLC-MK2 cell line...

Research Grants34

  1. Small heat shock proteins in smooth muscle plasticity
    William T Gerthoffer; Fiscal Year: 2013
    ..New developments in studies of microRNAs (miRNA) in phenotype determination suggest a convergence of p38MAPK/MK2 signaling and miRNA-induced silencing at the level of HSP27 and tristetraprolin...
  2. Mechanism of Anthrax Lethal Factor Toxicity
    Usamah S Kayyali; Fiscal Year: 2012
    ..We have shown that p38 MAP kinase activation leading to HSP27 phosphorylation by the kinase MK2 augments the endothelial permeability barrier through strengthening adhesive forces mediated by actin stress fiber ..
  3. MAP Kinase Signaling in Apoptosis-Induced Ventilator-Associated Lung Injury
    Mahendra Damarla; Fiscal Year: 2013
    ..Activation of p38 MAP kinase and its downstream effector MAPK-activated protein kinase MK2 (MK2) leads to phosphorylation of the small heat shock protein 27 (HSP27), a known regulator of apoptosis...
  4. SANDRA ANN REMPEL; Fiscal Year: 2015
    ....
  5. MOLECULAR MODELS OF IMMUNE-MEDIATED VASCULAR INJURY
    Jeffrey R Bender; Fiscal Year: 2012
    ..and mRNA stabilization, focusing on the Rac downstream targets p38 MAPK, MAPK-activated protein kinase 2 (MK2) and novel, T cell-specific MK2 targets, using pharmacologic inhibitors, RNA interference (RNAi)-based approaches, ..
  6. Michael B Yaffe; Fiscal Year: 2016
    ..We recently identified a third DNA damage response pathway mediated by p38MAPK and MAPKAP Kinase-2 (MK2) that is absolutely essential for p53-defective tumor cells to survive after DNA damage...
  7. Regulation of Neuronal Survival by the Rit GTPase
    DOUGLAS ALLEN ANDRES; Fiscal Year: 2012
    ..Importantly, data developed since the previous review suggests that a second novel Rit-p38-HSP27-MK2 pathway may stimulate AKT signaling to afford neuronal protection...
  8. Hypoxia-induced Endothelial Barrier Dysfunction
    Usamah Kayyali; Fiscal Year: 2009
    ..to the hypothesis that exposure to hypoxia initiates cell signaling through the p38 MAP kinase pathway involving MK2 and HSP27 activation that leads to actin cytoskeleton and hic-5 redistribution...
  9. Post-transcriptional control of inflammatory gene expression
    Thomas A Hamilton; Fiscal Year: 2013
    ..and (3) identification and definition of the signaling events coupling IL-17R and IL-1R stimulation with p38/MK2- independent mRNA stabilization...
  10. Mechanism of Myocardial Angiogenesis in Transgenic/Knockout Animals
    Nilanjana Maulik; Fiscal Year: 2010
    ..Aim 2. Genetically-engineered mice will be used such as MK2-/- to identify down stream target candidates of VEGF signaling through its receptors in IP as well as ischemic ..
  11. Atherogenic Induction of Neuroinflammation
    Narayan R Bhat; Fiscal Year: 2010
    ..e., MAPK-activated protein kinase 2 (i.e., MAPKAP K2 or MK2) to test the hypothesis that this pathway signals atherogenic induction of neurovascular and inflammatory ..
  12. Map Kinase Pathways and Anemia in the Elderly
    Leonidas C Platanias; Fiscal Year: 2010
    ....
  13. Smart Polymer Based MK2 Inhibitor to Suppress Vascular Graft Intimal Hyperplasia
    CRAIG LEWIS DUVALL; Fiscal Year: 2012
    ..We have identified MAPKAP kinase II (MK2) as a potential target for pharmacological intervention for preventing vascular graft IH...
  14. Keith L Kirkwood; Fiscal Year: 2015
    ..TTP is phosphorylated by the p38-MK2 pathway and may serve as a general mechanism of cytokine mRNA regulation...
  15. Yibin Wang; Fiscal Year: 2014
    ..Finally, we have demonstrated that a well established p38 downstream kinase MK2 has a selective contribution to p38 induced pathological changes in heart...
  16. Ventricular Remodeling in the Adapted Heart
    Nilanjana Maulik; Fiscal Year: 2012
    ..through either of its two tyrosine kinase receptors, VEGFR1/Flt-1 or VEGFR2/Flk1/KDR via its target protein MK2. In the present proposal we will use Flk-1 , Flt-1 and MK2-/- (MAPKAPKinase2) knockout mice in an attempt to ..
  17. Peptide therapeutics for inhibiting abdominal adhesions
    CYNTHIA ELLEN LANDER; Fiscal Year: 2012
    ..are known to initiate adhesion formation through activation of mitogen activated protein kinase activated kinase (MK2)...
  18. Role of cGMP in Ventilator-Induced Lung Endothelial Barrier Dysfunction
    David B Pearse; Fiscal Year: 2013
    ..from NO-stimulated sGC and 2) PDE2A expression through activation of p38 and MAP kinase-activated protein kinase 2 (MK2) resulting in accelerated hydrolysis of endothelial barrier protective cAMP...
  19. Correlation of Sendai Virus Mutations with Pathogenicity
    NANCY LUGENE MCQUEEN; Fiscal Year: 2012
    ..The only virus that was able to form plaques and undergo multiple rounds of replication in LLC-MK2 cells in the absence of trypsin, or cause a systemic infection in mice was RRGV0, a virus containing all F1-R M and ..
  20. MECHANISMS OF AIRWAY HYPERREACTIVITY
    William Gerthoffer; Fiscal Year: 2004
    ..3 and MKK6b, which are upstream activators of p38 MAPKs; MAP kinase activated protein kinase 2 (MK2), a substrate of p38 MAPK; and HSP27, a substrate of MK2 important in regulating the actin ..
  21. Herpresviral Gene Expression in Kaposis Sarcoma
    Don Ganem; Fiscal Year: 2010
    ..These studies will focus on (i) the interactions of kaposins B and C with components of the p38-MK2 pathway, and (ii) the identification and characterization of additional host signaling molecules and other factors ..
  22. Epidemiology of the Human Metapneumovirus, New-Haven CT
    Frank Esper; Fiscal Year: 2004
    ..Growth, propagation and cytopathic effects on LLC-MK2 cells will also be utilized...
  23. Anti-tick-borne Encephalitis Virus Miniprotein Agents
    Robert Fox; Fiscal Year: 2009
    ..The miniprotein MP-100 was shown to block infection of Vero and LLC-MK2 monkey kidney cell cultures by tick-borne LGT and Powassan viruses...
  24. Anti-tick-borne Encephalitis Virus Miniprotein Agents
    Robert Fox; Fiscal Year: 2003
    ..The miniprotein MP-100 was shown to block infection of Vero and LLC-MK2 monkey kidney cell cultures by tick-borne LGT and Powassan viruses...
  25. Antiviral Agents directed at West Nile Virus
    STEVEN WHITTEN; Fiscal Year: 2004
    ..The miniprotein MP-100 was shown to block infection of Vero and LLC-MK2 monkey kidney cell cultures by tick-borne Lgt and Powassan (POW) viruses...
  26. IMMUNOTHERAPY OF MELANOMA WITH ANTIIDIOTYPIC ANTIBODIES
    Soldano Ferrone; Fiscal Year: 1999
    ..i) anti-human high molecular weight-melanoma associated antigen (HMW-MAA) immunity elicited by mouse anti-id mAb MK2-23 in about 60% of patients with advanced melanoma is associated with regression of lesions in a few patients and ..
  27. Silencing Post Transcriptional Signaling Mechanisms in Periodontal Inflammation
    Keith Kirkwood; Fiscal Year: 2009
    ..TTP is phosphorylated by the p38-MK2 pathway and may serve as a general mechanism of cytokine mRNA regulation...
  28. MURINE ANTI-ID ANTIBODIES FOR IMMUNOTHERAPY OF MELANOMA
    Carl Hamby; Fiscal Year: 1992
    ..that conjugation to KLH and administration with an adjuvant markedly enhances the ability of the mouse anti-id MoAb MK2-23 to induce humoral and cellular immunity towards the HMW-MAA...
  29. Regulation and Function of MAPKAP Kinases
    Thomas Sturgill; Fiscal Year: 2005
    ..Aim four is to characterize the activation, enzymatic properties, and functions of MAPKAP kinase-like enzymes (Rcklp, Rck2p) in yeast, a model eukaryote, using combined biochemical and genetic approaches. ..
  30. Modulation of Neutrophil Apoptosis by Akt-Hsp27 Signalosome
    Madhavi Rane; Fiscal Year: 2008
    ..These studies will lead us to new targets for disrupting neutrophil-mediated tissue damage in inflammatory diseases. ..
  31. Altering Cytokine mRNA Stability in Periodontal Bone Loss
    Keith Kirkwood; Fiscal Year: 2008
    ..TTP is phosphorylated by the p38-MK2 pathway and may serve as a general mechanism of cytokine mRNA regulation...
  32. Role of Hsp27 in regulation of Neutrophil Apoptosis
    Madhavi Rane; Fiscal Year: 2006
    ..These studies will lead us to new targets for disrupting neutrophil-mediated tissue damage in inflammatory diseases. [unreadable] [unreadable] [unreadable]..
  33. Role of AP-2gamma in Ovarian Germline Stem Cells
    Quinton Winger; Fiscal Year: 2006
    ..The potential for developing successful treatments for human infertility provided by these experiments emphasizes the immediate importance for this research. ..
  34. Regulated Insulin Delivery From Tissue Engineering Skin*
    Keith Kirkwood; Fiscal Year: 2005
    ....